Highlights► Design of isotopic labeling experiments determines the precision of flux estimates. ► New approaches were developed to select optimal isotopic tracers and measurements. ► Tandem mass spectrometry provides more informative data for 13C flux analysis. ► Parallel labeling experiments can improve the quality of flux estimates. ► New methods are needed for optimal design of parallel labeling experiments. 相似文献
Highlights► New methods allow direct enrichment of palmitoylated proteins for LC–MS annotation. ► SILAC proteomics methods enhance the annotation of low abundance palmitoyl proteins. ► Alkynyl fatty acids facilitate pulse-chase labeling for measuring palmitoylation dynamics. ► Thioesterase inhibitors are promising new tools to dissect palmitoylation dynamics. 相似文献
Highlights► Peptide-based proteomics algorithms for ETD, HCD, and high-resolutions MS/MS spectra. ► Algorithms for identifying proteins in complex mixtures using shotgun proteomics. ► Spectral libraries and software pipelines for improved analytical throughput. ► Cloud computing to obtain results for large data sets. 相似文献
Highlights► Genome sequencing is an efficient, cost-effective way of characterizing microbes. ► Pangenome analysis gives new insight into texture formation in yoghurt. ► Pangenome analysis of probiotic strains has identified new effect or molecules. ► Pangenome analysis gives insight into probiotic–host specificity. ► Future perspectives include improved strains for food fermentations and probiotics. 相似文献
Highlights► Recent progress in biomolecular magic angle spinning solid state NMR is reviewed. ► MAS NMR is applied to study non-crystalline, non-soluble and high molecular-weight systems. ► New methods rely on sparse isotope enrichment, proton detection, fast spinning and signal enhancements. ► New structure calculations are based on 1H–1H restraints, paramagnetic effects and tensor analysis. ► Applications to protein aggregates, assemblies, fibrils, membrane proteins and enzymes are described. 相似文献
Highlights► Constraint-based modeling has been applied to the metabolism of single organisms. ► We summarize studies using this approach for microbe–microbe models. ► Host–microbe interactions can also be modeled with this approach. ► We also highlight current challenges in multi-organism modeling. ► This approach is very promising for studying the role of microbes in human health. 相似文献
Highlights► Health effects of dietary polyphenols often depend on multiple metabolic processes. ► Gut metabotypes with specific polyphenol metabolism patterns can be differentiated. ► Physiologically achievable concentrations of relevant metabolites should be tested. ► Novel products and applications can exploit polyphenol–microbiota interactions. 相似文献
Highlights► The hydraulic pathway of plants is vulnerable to develop air bubbles. ► Air bubble formation in plants is caused by drought or freeze–thaw events. ► Various mechanical xylem properties are correlated with drought-induced embolisms. ► Angiosperms have a greater ability to repair stem embolisms than gymnosperms. ► Secondarily woody shrubs are more embolism resistant than herbaceous relatives. 相似文献
Highlights► Access to proteomics is now possible after flow cytometry-based cell sorting. ► New visualization tools like Voronoi treemaps or streamgraphs now allows interpretation of complex omics data. ► Useful instrumentation to study microbial subpopulation from, for example, host–pathogen interaction assays. 相似文献
Highlights► This review covers current know-how on cheese flavor development. ► Proteolysis, autolysis and amino acid catabolism by lactic acid bacteria are discussed. ► Systems biology approaches will drive future technology improvements. ► Next-generation sequencing holds promise for fast increase in exploitable knowledge. ► Culture heterogeneity may be significant for industrial flavor production by LAB. 相似文献
Highlights► The plastidial pyruvate transporter in plants is driven by sodium symport. ► The mitochondrial pyruvate transporter MPC has been identified. ► All transporters needed for a synthetic PEP-CK/C4 photosynthetic cycle are now known. ► The SWEET proteins define a new class of sugar efflux carriers in plants. 相似文献
Highlights► We focus on the latest developments in nanotechnology-based electrophysiology. ► Methods for extracellular recording via nanoFET-based devices are described. ► Metal nanoelectrodes for the recording of electrogenic cells are reviewed. ► The latest developments of intracellular FET-based probes are shortly covered. ► Pioneering electrical scaffold devices for the interfacing with tissues are described. 相似文献
Highlights► FTIR spectroscopy for the analysis of the biochemical composition of phytoplankton. ► Cell separation by sorting for subsequent FTIR analysis. ► Measurement from subcommunities of natural phytoplankton samples. ► Physiological traits investigated by interpretation of FTIR spectra. ► Under the phytoplankton cell response to abiotic and biotic conditions. 相似文献
Highlights► Not all chelators are the same: more than metal sequestration and elimination. ► Metal chelates can redistribute metals across biological membranes. ► Metal-chelating fragments provide leads for metalloenzyme inhibition. ► Metal chelation can enhance metal reactivity and thus cytotoxicity. ► Prochelators provide site-activated metal chelation and passivation. 相似文献
Highlights► The action of industrial processes depends on microbial community structure. ► Microbes evolve very rapidly. ► Microbial evolution is affected by community structure. ► Community structure is affected by microbial evolution. ► This feedback may be crucial when considering microbial communities in industry. 相似文献
Highlights► Stable isotope-assisted metabolomics is powerful to determine metabolic fluxes. ► Stable isotope-assisted metabolomics is more complex for mammalian cell cultures. ► Biotechnological processes can be improved by assessing metabolic flux changes. ► Metabolic flux analysis is an attractive tool for toxicity studies. ► There is a huge potential for future applications on mammalian systems. 相似文献
Highlights► Microbial production of pharmaceutical and fuel molecules. ► Systems biology tools advanced our understanding about synthetic pathway design. ► Synthetic biology led us to cell factories tailor-made for chemical production. ► Combinatorial gene assembly for synthetic pathway diversification. ► Engineering static, dynamic and spatial control for better production phenotype. 相似文献
Highlights► Hybridization chain reactions for amplified DNA detection and DNA assembly. ► Rolling circle amplification-synthesized DNAzyme chains for amplified DNA detection. ► Bi-enzyme cascades and biocatalytic synthesis of metallic nanowires on DNA templates. ► Self-assembled one-dimensional DNA nanostructures for biocatalytic cascades. ► Programmed assembly of metal nanoparticles on one-dimensional DNA nanostructures. 相似文献
Highlights► The irreversible inactivation of protein targets by metal complexes is described. ► The article summaries catalysts that hydrolyze or oxidize proteins. ► Cyclen-based catalysts that selectively hydrolyze peptides and proteins are described. ► Catalysts that generate reactive oxygen species are important for protein inactivation. ► Metal-based oxidants are potent and selective agents for protein inactivation. 相似文献
