Highlights► Microbial production of pharmaceutical and fuel molecules. ► Systems biology tools advanced our understanding about synthetic pathway design. ► Synthetic biology led us to cell factories tailor-made for chemical production. ► Combinatorial gene assembly for synthetic pathway diversification. ► Engineering static, dynamic and spatial control for better production phenotype. 相似文献
Highlights► The plastidial pyruvate transporter in plants is driven by sodium symport. ► The mitochondrial pyruvate transporter MPC has been identified. ► All transporters needed for a synthetic PEP-CK/C4 photosynthetic cycle are now known. ► The SWEET proteins define a new class of sugar efflux carriers in plants. 相似文献
Highlights► Transport engineering has the potential to increase nutritional value of crops. ► Transport engineering can increase yield of synthetic biology approaches. ► Neo-functionalization of primary metabolite transporters to specialized metabolism. ► Elimination of glucosinolate seed accumulation is achieved via transport engineering. ► Glucosinolates in Arabidopsis: a model for transport of specialized metabolites. 相似文献
Highlights► Engineered feedback loops implement elaborate functionality in synthetic circuits. ► The homeostatic potential of feedback loops is under-utilized in synthetic biology. ► Feedback control of engineered or natural cellular functions can be achieved using biological components or in silico regulation. ► Optogenetics and microfluidic technologies are important for implementing in silico control. 相似文献
Highlights► The application of Pichia pastoris for biopharmaceutical production is described. ► Synthetic biology approaches and perspectives to improve production are reviewed. ► Glycoengineering efforts to produce humanized, uniform glycoproteins are covered. ► The design and application of synthetic promoter variants are highlighted. ► The molecular toolbox available for synthetic biology in P. pastoris is discussed. 相似文献
Semi-synthetic minimal cells are constructed by encapsulating the minimal number of nucleic acids, enzymes and low molecular-weight compounds inside lipid vesicles (liposomes) in order to create a cell-like system.
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Highlights► Minimal synthetic cells are used in origin of life studies and synthetic biology. ► The semi-synthetic approach is based on cell-free and liposome technology. ► Solutes can be super-encapsulated inside vesicles against the expectations. ► There have been new attempts to construct self-reproducing synthetic cells. ► Vesicle fusion and vesicle colonies emphasize the importance of cooperation. 相似文献
Highlights► Metabolic and regulatory networks are finely tuned for biodegradation of aromatics. ► New pathways and widespread bacterial biodegradation capabilities revealed by omics. ► Full characterization of hybrid pathways expands the scope of aromatic biodegradation. ► The metabolism of aromatics plays a pivotal role in cell to cell communication. ► Computational and synthetic biology approaches design novel biodegradation pathways. 相似文献
Highlights► RiPPs have high capabilities of target interaction far beyond the field of anti-infectives. ► RiPPs are especially suitable for design and engineering with the genetic code expansion. ► First published studies yielded RiPPs with unnatural/synthetic amino acids. ► Achievement: expanded scope of the in vivo (mainly bacterial) RiPPs synthesis. ► Perspective: design of RiPPS with novel chemistries and unique sequence combinations. 相似文献
Highlights► Functional polymer nanostructures as efficient supports for confined catalysis. ► Polymer scaffold can provide protection of active catalyst for prolonged activity. ► Hydrophobic cavities allows for efficient organic reactions to take place in water. ► Stimuli-responsive polymers can provide control over nanoreactor catalytic activity. ► Folding of single polymer chains show potential for future enzyme mimic systems. 相似文献
Highlights► Recent advances have been made on plant genome-scale metabolic reconstruction. ► Cellular compartmentation makes plant genome-scale reconstruction challenging. ► Current reconstructions capture important features of plant metabolism. ► The models have been used to study isolated tissues and tissue interaction. ► We review the challenges and potential of plant reconstruction and modelling. 相似文献
Highlights► Poly(ω-hydroxy fatty acids) are polyethylene-like biobased polyester. ► Medium-chain-length polyhydroxyalkanoate homopolymers are processed to stretchy film. ► Microbial lactate-based polyesters exhibit transparent and flexible properties. ► Enzyme and metabolic engineering facilitates the expanding of bio-based polymers. 相似文献
Highlights► Crop yield depends on efficiency of conversion of light energy into biomass. ► Models have estimated energy conversion efficiency in photosynthesis and metabolism. ► Research is exploring strategies to modify or suppress photorespiration. ► Energy loss during conversion of photosynthate to biomass is also large ► Research is addressing uncertainties over where these losses occur. 相似文献
Highlights► The intestinal microbiome is recognized as an important determinant of health. ► Galacto-oligosaccharides are non-digestible carbohydrates. ► Galacto-oligosaccharides stimulate growth and activity of gut bacteria. ► Subtle differences in structure can result in significant differences in activity. ► Less abundant oligosaccharides from bovine milk are similar to those from human milk. 相似文献
Highlights► Alkylating enzymes allow regiospecific and chemospecific reactions where classical chemical syntheses fail or require an extensive protection strategy. ► Prenyltransferase and methyltransferase are increasingly applicable in biotechnology. ► Cofactor or cosubstrate availability can still be a limiting factor. ► Enzyme cascade reactions and/or whole-cell systems are applied to perform multiple step transformations. ► Alkyltransferases are crucial elements in pathway engineering and synthetic biology, especially toward plant derived (natural) products. 相似文献
Highlights► The action of industrial processes depends on microbial community structure. ► Microbes evolve very rapidly. ► Microbial evolution is affected by community structure. ► Community structure is affected by microbial evolution. ► This feedback may be crucial when considering microbial communities in industry. 相似文献
Highlights► Ethanol could hinder BTEX attenuation by various biogeochemical mechanisms. ► O2 depletion, enzyme repression and metabolic flux dilution elongate BTEX plumes. ► Elongation is site-specific and is offset by fortuitous growth of BTEX degraders. ► Ethanol migrates through the capillary fringe, a zone of high microbial activity. ► Ethanol-derived CH4 could pose an explosion risk and enhance BTEX vapor intrusion. 相似文献
Highlights► The exposure of bacteria to pollutants induces chemoattraction or chemorepellence. ► Pollutant chemoreceptors so far identified are present on plasmids. ► Chemotaxis increases pollutant bioavailability and enhances biodegradation rates. ► Chemotaxis may improve microbial inoculants designed just on catabolic potential. 相似文献
Highlights► Production of novel pharmaceutical and commodity chemicals. ► Protein and pathway engineering pave the way for product diversifications. ► ‘Omics’ data have helped assembly of pathways from different species. ► Recombination of enzymatic domains and site-directed mutagenesis. ► Tunable control of pathways for better production. 相似文献
Highlights► We focus on the latest developments in nanotechnology-based electrophysiology. ► Methods for extracellular recording via nanoFET-based devices are described. ► Metal nanoelectrodes for the recording of electrogenic cells are reviewed. ► The latest developments of intracellular FET-based probes are shortly covered. ► Pioneering electrical scaffold devices for the interfacing with tissues are described. 相似文献
