Highlights► Genome sequencing is an efficient, cost-effective way of characterizing microbes. ► Pangenome analysis gives new insight into texture formation in yoghurt. ► Pangenome analysis of probiotic strains has identified new effect or molecules. ► Pangenome analysis gives insight into probiotic–host specificity. ► Future perspectives include improved strains for food fermentations and probiotics. 相似文献
Highlights► We focus on the latest developments in nanotechnology-based electrophysiology. ► Methods for extracellular recording via nanoFET-based devices are described. ► Metal nanoelectrodes for the recording of electrogenic cells are reviewed. ► The latest developments of intracellular FET-based probes are shortly covered. ► Pioneering electrical scaffold devices for the interfacing with tissues are described. 相似文献
Highlights► Virus-based nanoparticles provide unique traits for nanoparticle applications. ► We discuss prevailing covalent functionalization strategies for VNPs. ► We discuss recent progress in noncanonical amino acid applications in VNPs. 相似文献
Highlights► Genome-wide association studies with metabolomics constitute mGWAS. ► mGWAS provide insights into genetic and environmental impact on metabolic processes. ► We review essential strategies for mGWAS. ► Examples of mGWAS in large cohort studies are discussed. 相似文献
Highlights► FTIR spectroscopy for the analysis of the biochemical composition of phytoplankton. ► Cell separation by sorting for subsequent FTIR analysis. ► Measurement from subcommunities of natural phytoplankton samples. ► Physiological traits investigated by interpretation of FTIR spectra. ► Under the phytoplankton cell response to abiotic and biotic conditions. 相似文献
Highlights► Recent advances have been made on plant genome-scale metabolic reconstruction. ► Cellular compartmentation makes plant genome-scale reconstruction challenging. ► Current reconstructions capture important features of plant metabolism. ► The models have been used to study isolated tissues and tissue interaction. ► We review the challenges and potential of plant reconstruction and modelling. 相似文献
Highlights► Network analysis is essential for data mining of omics-based large-scale data sets. ► Gene coexpression analysis is useful for prediction of gene function. ► Comparative network analysis can reveal common and unique plant metabolic pathways. ► Novel genome editing tools facilitate rational metabolic engineering. 相似文献
Highlights► Combining networks and proteomics can overcome consistency/coverage issues. ► Differential subnets are more robust biomarkers than single genes. ► Utilizing networks allows unprecedented class discovery/hypothesis testing/analysis. ► Novel proteomics-specific network methods that address limitations are needed. 相似文献
Highlights► Stable isotope-assisted metabolomics is powerful to determine metabolic fluxes. ► Stable isotope-assisted metabolomics is more complex for mammalian cell cultures. ► Biotechnological processes can be improved by assessing metabolic flux changes. ► Metabolic flux analysis is an attractive tool for toxicity studies. ► There is a huge potential for future applications on mammalian systems. 相似文献
Highlights► Fluorescence nanoscopy can be used to decipher subcellular structures in biological systems. ► We review the underlying principles and biological application of STED, PALM, and STORM. ► For super-resolution imaging methods, there is a tradeoff between temporal and spatial resolution. ► Improving probe brightness and probe photostability are key factors for achieving higher resolution. 相似文献
Highlights► Constraint-based modeling has been applied to the metabolism of single organisms. ► We summarize studies using this approach for microbe–microbe models. ► Host–microbe interactions can also be modeled with this approach. ► We also highlight current challenges in multi-organism modeling. ► This approach is very promising for studying the role of microbes in human health. 相似文献
Highlights► We review modern advances to characterize biological nano-complexes using X-ray scattering. ► X-ray scattering is used to study both the structures and macro-molecular interactions. ► Contemporary techniques include integration of complementary techniques together with X-ray scattering. ► Novel computational and technological advances, including coherent scattering technique, will be discussed. 相似文献
Highlights► A powerful range of tools has been developed for metabolic network flux analysis. ► These tools yield insights that are used to aid microbial metabolic engineering. ► Plants present great opportunities and special challenges to applying these tools. ► Tool selection and knowledge of plant systems is key to practical success. 相似文献
Highlights► Bioconductor proposes more than 20 R packages for FCM analysis. ► Data infrastructure proposed by flowCore has become the standard for many packages. ► Automated gating is improving but remains an intensive field of research. 相似文献
Highlights► Microalgal oils have clear potential to replace functions of major vegetable oils. ► A limited production surface is required to produce food commodities by microalgae. ► Major breakthroughs are required to enable cost effective production of microalgae. ► Life cycle analysis can provide direction for sustainable production of microalgae. ► Safety of food ingredients from microalgae should be confirmed. 相似文献
Highlights► The emerging field of single-cell protein analytics is reviewed. ► Antibody-based, fluorescence-based and mass spectrometry-based approaches are discussed. ► We present a workflow for the combination of flow cytometry and mass spectrometry. ► This allows monitoring of population heterogeneity on the proteomic level. 相似文献
Highlights► Electrofuels are non-photosynthetic, energy-dense fuels made from carbon dioxide. ► Herein, we review electrofuel production in hydrogen-utilizing organisms. ► We review both current and emerging electrofuel platforms and organisms. ► New advancements in genetic tools for electrofuel host organisms are examined. 相似文献
