Highlights► Comparing biofuel crops is exceedingly difficult using conventional measurement methods. ► The PETRO approach quantitatively compares biological matter in terms of energy and carbon. ► Tracking carbon flux from air to liquid fuel compares all biofuel crop yields on a common basis. ► Reporting energy yields (GJ ha−1 y−1) and carbon flux (MgC ha−1 y−1) enables regional comparison. 相似文献
Highlights► Hybridization chain reactions for amplified DNA detection and DNA assembly. ► Rolling circle amplification-synthesized DNAzyme chains for amplified DNA detection. ► Bi-enzyme cascades and biocatalytic synthesis of metallic nanowires on DNA templates. ► Self-assembled one-dimensional DNA nanostructures for biocatalytic cascades. ► Programmed assembly of metal nanoparticles on one-dimensional DNA nanostructures. 相似文献
Highlights► Access to proteomics is now possible after flow cytometry-based cell sorting. ► New visualization tools like Voronoi treemaps or streamgraphs now allows interpretation of complex omics data. ► Useful instrumentation to study microbial subpopulation from, for example, host–pathogen interaction assays. 相似文献
Highlights► A powerful range of tools has been developed for metabolic network flux analysis. ► These tools yield insights that are used to aid microbial metabolic engineering. ► Plants present great opportunities and special challenges to applying these tools. ► Tool selection and knowledge of plant systems is key to practical success. 相似文献
Highlights► Enzymes can be directed into compartments to create nanobioreactors in bacteria. ► Crystal structures of shell proteins suggest that pores enable selective diffusion. ► Specific peptide sequences target heterologous proteins for encapsulation. 相似文献
Highlights► Poly(ω-hydroxy fatty acids) are polyethylene-like biobased polyester. ► Medium-chain-length polyhydroxyalkanoate homopolymers are processed to stretchy film. ► Microbial lactate-based polyesters exhibit transparent and flexible properties. ► Enzyme and metabolic engineering facilitates the expanding of bio-based polymers. 相似文献
Highlights► The irreversible inactivation of protein targets by metal complexes is described. ► The article summaries catalysts that hydrolyze or oxidize proteins. ► Cyclen-based catalysts that selectively hydrolyze peptides and proteins are described. ► Catalysts that generate reactive oxygen species are important for protein inactivation. ► Metal-based oxidants are potent and selective agents for protein inactivation. 相似文献
Highlights► Peptide-based proteomics algorithms for ETD, HCD, and high-resolutions MS/MS spectra. ► Algorithms for identifying proteins in complex mixtures using shotgun proteomics. ► Spectral libraries and software pipelines for improved analytical throughput. ► Cloud computing to obtain results for large data sets. 相似文献
Highlights► This review focuses on the application of protein based nanomaterials for the energy application. ► Assembled peptides and engineered proteins. ► Hybrid structures of protein and inorganic nanomaterials. ► Supramolecular structures of proteins for energy application. 相似文献
Highlights► Thiol compounds (glutathione) protect LAB from multiple environmental stresses. ► Engineering antioxidative properties of LAB can improve the overall robustness. ► Genomics approaches helped to reveal the molecular mechanism of stress response. 相似文献
Highlights► Responsive MRI contrast agents enable the study of biochemical events. ► Recent Gd-based contrast agents are reviewed here. ► Responsive agents act by modulating hydration state, molecular tumbling or number of metal centres. ► Promising strategies for future probe design are identified. 相似文献
Highlights► Development of prodrug-based self-assembled hydrogels as a new class of biomaterials. ► Design of prodrugs that can self-assemble to form hydrogels. ► These hydrogels overcome limitations such as low-drug loading and burst release of drugs. ► Locally injectable self-assembled hydrogels exhibit potential as effective therapeutics. 相似文献
Highlights► Nanopores are single-molecule detectors with exceptional sensitivity and versatility. ► Over the past few years, progress has been made in making and using nanopores in biotechnology. ► Nanopore-based DNA sequencing can revolutionize healthcare by reducing both sequencing costs and time. 相似文献
Highlights► Fermentation is being adopted by many companies for new and natural products. ► Fermentation is increasingly used as sustainable alternative for existing food processing. ► Innovation is achieved by applying traditional fermentation processes for novel substrates. 相似文献
Highlights► Network analysis is essential for data mining of omics-based large-scale data sets. ► Gene coexpression analysis is useful for prediction of gene function. ► Comparative network analysis can reveal common and unique plant metabolic pathways. ► Novel genome editing tools facilitate rational metabolic engineering. 相似文献
Highlights► Phylogeny of Saccharomyces cerevisiae reveals industry-relevant groupings. ► Comparative genomics reveals genomic variation across industrial yeast strains. ► Next-gen sequencing in next-gen wine yeast strain development. ► Precise mapping of QTLs for marker-assisted breeding of industrial yeast. ► Discovery of putative mobile gene cluster that uses novel type of transposition. 相似文献
Highlights► Not all chelators are the same: more than metal sequestration and elimination. ► Metal chelates can redistribute metals across biological membranes. ► Metal-chelating fragments provide leads for metalloenzyme inhibition. ► Metal chelation can enhance metal reactivity and thus cytotoxicity. ► Prochelators provide site-activated metal chelation and passivation. 相似文献
