Highlights► Microbial production of pharmaceutical and fuel molecules. ► Systems biology tools advanced our understanding about synthetic pathway design. ► Synthetic biology led us to cell factories tailor-made for chemical production. ► Combinatorial gene assembly for synthetic pathway diversification. ► Engineering static, dynamic and spatial control for better production phenotype. 相似文献
Highlights► Engineered feedback loops implement elaborate functionality in synthetic circuits. ► The homeostatic potential of feedback loops is under-utilized in synthetic biology. ► Feedback control of engineered or natural cellular functions can be achieved using biological components or in silico regulation. ► Optogenetics and microfluidic technologies are important for implementing in silico control. 相似文献
Highlights► Transport engineering has the potential to increase nutritional value of crops. ► Transport engineering can increase yield of synthetic biology approaches. ► Neo-functionalization of primary metabolite transporters to specialized metabolism. ► Elimination of glucosinolate seed accumulation is achieved via transport engineering. ► Glucosinolates in Arabidopsis: a model for transport of specialized metabolites. 相似文献
Highlights► This review covers current know-how on cheese flavor development. ► Proteolysis, autolysis and amino acid catabolism by lactic acid bacteria are discussed. ► Systems biology approaches will drive future technology improvements. ► Next-generation sequencing holds promise for fast increase in exploitable knowledge. ► Culture heterogeneity may be significant for industrial flavor production by LAB. 相似文献
Highlights► Metabolic and regulatory networks are finely tuned for biodegradation of aromatics. ► New pathways and widespread bacterial biodegradation capabilities revealed by omics. ► Full characterization of hybrid pathways expands the scope of aromatic biodegradation. ► The metabolism of aromatics plays a pivotal role in cell to cell communication. ► Computational and synthetic biology approaches design novel biodegradation pathways. 相似文献
Semi-synthetic minimal cells are constructed by encapsulating the minimal number of nucleic acids, enzymes and low molecular-weight compounds inside lipid vesicles (liposomes) in order to create a cell-like system.
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Highlights► Minimal synthetic cells are used in origin of life studies and synthetic biology. ► The semi-synthetic approach is based on cell-free and liposome technology. ► Solutes can be super-encapsulated inside vesicles against the expectations. ► There have been new attempts to construct self-reproducing synthetic cells. ► Vesicle fusion and vesicle colonies emphasize the importance of cooperation. 相似文献
Highlights► Alkylating enzymes allow regiospecific and chemospecific reactions where classical chemical syntheses fail or require an extensive protection strategy. ► Prenyltransferase and methyltransferase are increasingly applicable in biotechnology. ► Cofactor or cosubstrate availability can still be a limiting factor. ► Enzyme cascade reactions and/or whole-cell systems are applied to perform multiple step transformations. ► Alkyltransferases are crucial elements in pathway engineering and synthetic biology, especially toward plant derived (natural) products. 相似文献
Highlights► RiPPs have high capabilities of target interaction far beyond the field of anti-infectives. ► RiPPs are especially suitable for design and engineering with the genetic code expansion. ► First published studies yielded RiPPs with unnatural/synthetic amino acids. ► Achievement: expanded scope of the in vivo (mainly bacterial) RiPPs synthesis. ► Perspective: design of RiPPS with novel chemistries and unique sequence combinations. 相似文献
Highlights► The plastidial pyruvate transporter in plants is driven by sodium symport. ► The mitochondrial pyruvate transporter MPC has been identified. ► All transporters needed for a synthetic PEP-CK/C4 photosynthetic cycle are now known. ► The SWEET proteins define a new class of sugar efflux carriers in plants. 相似文献
Highlights► Ammonia lyases and aminomutases are emerging in green synthetic routes to chiral amines. ► An ammonia lyase is being explored as an enzyme therapeutic for treating phenylketonuria and cancer. ► The restricted substrate range of the wild-type enzymes limits their widespread application. ► The non-reliance on external cofactors and direct functionalization of an olefinic bond make ammonia lyases attractive biocatalysts for use in the synthesis of natural and non-natural amino acids. ► The approach of combining structure-guided enzyme engineering with efficient mutant library screening has broadened the synthetic scope of these biocatalysts. 相似文献
Highlights► Nucleic acids can be used to build reaction modules modeled after in vivo or in silico computers. ► In vitro circuits built from these modules perform one-shot calculations or show complex dynamics. ► Applications at the interface with biology, or for molecular-scale robotics, are burgeoning. 相似文献
Highlights► Highly variable and complex pathogens remain challenging vaccine targets. ► Systems biology and reverse vaccinology continue to foster success. ► New ways of presenting conserved, functionally critical epitopes hold promise. ► Adjuvant systems allow for enduring, native antigen presentation. ► An ideal vaccine provides synergy between innate, cellular, and humoral immunity. 相似文献
Highlights► Factors that limit bacteriocin application in the food industry may be addressed in a number of ways. ► Conjugation is widely exploited to facilitate bacteriocin production by specific LAB. ► Genetic manipulation can generate LAB producing greater concentrations and bacteriocin numbers. ► Bioengineering can greatly increase LAB bacteriocin activity and inhibitory spectra. 相似文献
Highlights► Metabolic dependencies that arise in tumors may present new opportunities for therapy. ► Advanced methods in metabolic systems biology can help identify these dependencies. ► Application of isotope tracers to cancer models can facilitates quantitation of fluxes. ► Tumors with compromised TCA metabolism exhibit unique metabolic profiles. 相似文献
Highlights► Single cell metabolomics provides information on the heterogeneity of populations of cells. ► Current technologies allow hundreds of metabolites to be measured from a single cell. ► Quantitative metabolome information at the single cell level aids systems biology modeling. ► Sample preparation remains a significant challenge in single cell metabolomics. 相似文献
Highlights► Microalgal oils have clear potential to replace functions of major vegetable oils. ► A limited production surface is required to produce food commodities by microalgae. ► Major breakthroughs are required to enable cost effective production of microalgae. ► Life cycle analysis can provide direction for sustainable production of microalgae. ► Safety of food ingredients from microalgae should be confirmed. 相似文献
Highlights► Microbial catalysts with different PCB dechlorinating activities are cultured. ► Biostimulation and bioaugmentation has been successful in the laboratory. ► Molecular tools for monitoring dechlorinating bacteria in situ are available. ► Methods to deploy these catalysts in the field are currently under development. 相似文献
Highlights► This review focuses on the application of protein based nanomaterials for the energy application. ► Assembled peptides and engineered proteins. ► Hybrid structures of protein and inorganic nanomaterials. ► Supramolecular structures of proteins for energy application. 相似文献
Highlights► Bacterial glycoproteins are potential drug targets and are worthy of study. ► Metabolic glycan labeling enables discovery and characterization of glycoproteins. ► Lectin arrays permit monitoring of bacterial glycoprotein dynamics. ► Crosslinking sugars reveal binding partners of glycoproteins to elucidate function. ► Bacterial glycosylation systems facilitate efficient glycoprotein production. 相似文献
