Highlights► The application of Pichia pastoris for biopharmaceutical production is described. ► Synthetic biology approaches and perspectives to improve production are reviewed. ► Glycoengineering efforts to produce humanized, uniform glycoproteins are covered. ► The design and application of synthetic promoter variants are highlighted. ► The molecular toolbox available for synthetic biology in P. pastoris is discussed. 相似文献
Highlights► In vivo flux distributions can be determined with increasing accuracy and breadth. ► Enzyme activity can partly be identified from codon usage and other DNA features. ► Stoichiometric constraints of metabolism set a frame that can be exploited to design efficient metabolic networks. ► Insights into DNA-sequence–function relationships lead to first examples of rational DNA writing. ► Increasing DNA synthesis speed moves metabolic engineering from tinkering to green field design. 相似文献
Highlights► Microorganisms play a crucial role in detoxifying environmental pollutants. ► Proving that biodegradation processes occur in the real world (in situ) is important. ► Genetics, biochemistry, and physiology advance the science of microbial metabolism. ► Such advances provide new molecular-biomarker tools for documenting biodegradation. ► Applying new tools to contaminated sites reveals new microbial processes and diversity. 相似文献
Highlights► Microbial production of pharmaceutical and fuel molecules. ► Systems biology tools advanced our understanding about synthetic pathway design. ► Synthetic biology led us to cell factories tailor-made for chemical production. ► Combinatorial gene assembly for synthetic pathway diversification. ► Engineering static, dynamic and spatial control for better production phenotype. 相似文献
Highlights► Microbial catalysts with different PCB dechlorinating activities are cultured. ► Biostimulation and bioaugmentation has been successful in the laboratory. ► Molecular tools for monitoring dechlorinating bacteria in situ are available. ► Methods to deploy these catalysts in the field are currently under development. 相似文献
Highlights► Stable isotopes provide insights about in situ biodegradation mechanisms and rates. ► Isotopic approaches can be confounded by abiotic processes and heterogeneity effects. ► Isotopic applications are improving with new methods of analysis and modeling. ► Isotopic data are most useful when combined with other approaches for fate assessment. 相似文献
Highlights► Magnetic nanoparticles (MNPs) can be in engineered and functionalized. ► MNPs can be used as magnetic resonance imaging contrast agents and targeted MNPs can be used in theranostic applications. ► MNPs are useful for in vivo drug and gene delivery. ► MNPs are useful for photodynamic, phototherapy and hyperthermia. ► MNPs can be used for controlled released and manipulation of cell in vivo. 相似文献
Highlights► Engineered feedback loops implement elaborate functionality in synthetic circuits. ► The homeostatic potential of feedback loops is under-utilized in synthetic biology. ► Feedback control of engineered or natural cellular functions can be achieved using biological components or in silico regulation. ► Optogenetics and microfluidic technologies are important for implementing in silico control. 相似文献
Highlights► Phylogeny of Saccharomyces cerevisiae reveals industry-relevant groupings. ► Comparative genomics reveals genomic variation across industrial yeast strains. ► Next-gen sequencing in next-gen wine yeast strain development. ► Precise mapping of QTLs for marker-assisted breeding of industrial yeast. ► Discovery of putative mobile gene cluster that uses novel type of transposition. 相似文献
Highlights► Microbial reduction of U(VI) can remove uranium from contaminated groundwater. ► Concerns about the stability of precipitated uranium need further study. ► Environmental omics is useful for diagnosing in situ physiological state of microbes. ► Genome-scale modeling of in situ activity of bioremediation organisms is possible. 相似文献
Highlights► Responsive MRI contrast agents enable the study of biochemical events. ► Recent Gd-based contrast agents are reviewed here. ► Responsive agents act by modulating hydration state, molecular tumbling or number of metal centres. ► Promising strategies for future probe design are identified. 相似文献
Highlights► This review describes metabolomic studies of anaerobic hydrocarbon-impacted sites. ► The discovery of new pathways has provided new metabolic and molecular biomarkers. ► Assessment of in situ biodegradation is enhanced via integrative approaches. 相似文献
Highlights► The expansion of shape space and growth of interest in structural DNA nanotechnology. ► Recent advances that support the design and practical fabrication of in particular DNA origami objects. ► DNA-based devices that support scientific discovery in different fields of science. 相似文献
Highlights► Design of isotopic labeling experiments determines the precision of flux estimates. ► New approaches were developed to select optimal isotopic tracers and measurements. ► Tandem mass spectrometry provides more informative data for 13C flux analysis. ► Parallel labeling experiments can improve the quality of flux estimates. ► New methods are needed for optimal design of parallel labeling experiments. 相似文献
Highlights► Metabolic and regulatory networks are finely tuned for biodegradation of aromatics. ► New pathways and widespread bacterial biodegradation capabilities revealed by omics. ► Full characterization of hybrid pathways expands the scope of aromatic biodegradation. ► The metabolism of aromatics plays a pivotal role in cell to cell communication. ► Computational and synthetic biology approaches design novel biodegradation pathways. 相似文献
Highlights► We focus on the latest developments in nanotechnology-based electrophysiology. ► Methods for extracellular recording via nanoFET-based devices are described. ► Metal nanoelectrodes for the recording of electrogenic cells are reviewed. ► The latest developments of intracellular FET-based probes are shortly covered. ► Pioneering electrical scaffold devices for the interfacing with tissues are described. 相似文献
Highlights► Poly(ω-hydroxy fatty acids) are polyethylene-like biobased polyester. ► Medium-chain-length polyhydroxyalkanoate homopolymers are processed to stretchy film. ► Microbial lactate-based polyesters exhibit transparent and flexible properties. ► Enzyme and metabolic engineering facilitates the expanding of bio-based polymers. 相似文献
Highlights► Not all chelators are the same: more than metal sequestration and elimination. ► Metal chelates can redistribute metals across biological membranes. ► Metal-chelating fragments provide leads for metalloenzyme inhibition. ► Metal chelation can enhance metal reactivity and thus cytotoxicity. ► Prochelators provide site-activated metal chelation and passivation. 相似文献
