Highlights► Photocrosslinking provides insight into context-dependent interactions. ► Interactome dynamics can be determined by photocrosslinking. ► Use of photocrosslinkers defines specific interaction interfaces within large complexes. ► Crosslinking captures transient interaction complexes containing IDPs and glycoproteins. 相似文献
Highlights► The application of Pichia pastoris for biopharmaceutical production is described. ► Synthetic biology approaches and perspectives to improve production are reviewed. ► Glycoengineering efforts to produce humanized, uniform glycoproteins are covered. ► The design and application of synthetic promoter variants are highlighted. ► The molecular toolbox available for synthetic biology in P. pastoris is discussed. 相似文献
Highlights► We focus on the latest developments in nanotechnology-based electrophysiology. ► Methods for extracellular recording via nanoFET-based devices are described. ► Metal nanoelectrodes for the recording of electrogenic cells are reviewed. ► The latest developments of intracellular FET-based probes are shortly covered. ► Pioneering electrical scaffold devices for the interfacing with tissues are described. 相似文献
Highlights► Poly(ω-hydroxy fatty acids) are polyethylene-like biobased polyester. ► Medium-chain-length polyhydroxyalkanoate homopolymers are processed to stretchy film. ► Microbial lactate-based polyesters exhibit transparent and flexible properties. ► Enzyme and metabolic engineering facilitates the expanding of bio-based polymers. 相似文献
Highlights► Not all chelators are the same: more than metal sequestration and elimination. ► Metal chelates can redistribute metals across biological membranes. ► Metal-chelating fragments provide leads for metalloenzyme inhibition. ► Metal chelation can enhance metal reactivity and thus cytotoxicity. ► Prochelators provide site-activated metal chelation and passivation. 相似文献
Highlights► The intestinal microbiome is recognized as an important determinant of health. ► Galacto-oligosaccharides are non-digestible carbohydrates. ► Galacto-oligosaccharides stimulate growth and activity of gut bacteria. ► Subtle differences in structure can result in significant differences in activity. ► Less abundant oligosaccharides from bovine milk are similar to those from human milk. 相似文献
Highlights► The different types of MTBE and TBA-degrading microorganisms are summarized. ► The pathways involved in aerobic and anaerobic MTBE biodegradation are described. ► The roles of key and novel enzymes in these processes are highlighted. ► The impacts of biochemical studies on stable isotope-based analyses are discussed. 相似文献
Highlights► Genome-wide association studies with metabolomics constitute mGWAS. ► mGWAS provide insights into genetic and environmental impact on metabolic processes. ► We review essential strategies for mGWAS. ► Examples of mGWAS in large cohort studies are discussed. 相似文献
Highlights► This review covers current know-how on cheese flavor development. ► Proteolysis, autolysis and amino acid catabolism by lactic acid bacteria are discussed. ► Systems biology approaches will drive future technology improvements. ► Next-generation sequencing holds promise for fast increase in exploitable knowledge. ► Culture heterogeneity may be significant for industrial flavor production by LAB. 相似文献
Highlights► The plastidial pyruvate transporter in plants is driven by sodium symport. ► The mitochondrial pyruvate transporter MPC has been identified. ► All transporters needed for a synthetic PEP-CK/C4 photosynthetic cycle are now known. ► The SWEET proteins define a new class of sugar efflux carriers in plants. 相似文献
Highlights► The irreversible inactivation of protein targets by metal complexes is described. ► The article summaries catalysts that hydrolyze or oxidize proteins. ► Cyclen-based catalysts that selectively hydrolyze peptides and proteins are described. ► Catalysts that generate reactive oxygen species are important for protein inactivation. ► Metal-based oxidants are potent and selective agents for protein inactivation. 相似文献
Highlights► Combining networks and proteomics can overcome consistency/coverage issues. ► Differential subnets are more robust biomarkers than single genes. ► Utilizing networks allows unprecedented class discovery/hypothesis testing/analysis. ► Novel proteomics-specific network methods that address limitations are needed. 相似文献
Highlights► Responsive MRI contrast agents enable the study of biochemical events. ► Recent Gd-based contrast agents are reviewed here. ► Responsive agents act by modulating hydration state, molecular tumbling or number of metal centres. ► Promising strategies for future probe design are identified. 相似文献
Highlights► The exposure of bacteria to pollutants induces chemoattraction or chemorepellence. ► Pollutant chemoreceptors so far identified are present on plasmids. ► Chemotaxis increases pollutant bioavailability and enhances biodegradation rates. ► Chemotaxis may improve microbial inoculants designed just on catabolic potential. 相似文献
Highlights► Microbial interactions in food fermentations are crucial for product functionality. ► Complex microbial consortia tolerate more variation in the environment. ► Knowledge of communities of fermenting microbes drives diversification of starter cultures. ► Use of complex starter cultures requires new propagation methodologies. ► Immobilised cell technology is a high potential solution for propagating complex cultures. 相似文献
Highlights► Specialized metabolism offers challenging engineering opportunities. ► Unlike central metabolism, specialized metabolism is slow, sluggish and messy. ► Spontaneous reactions are relatively common in specialized metabolism. ► Enzymes can be engineered for higher rate and substrate specificity. ► Enzymes can be engineered for naturally spontaneous reactions. 相似文献
Highlights► Nanotechnologies are used to mimic natural components of the cell microenvironment. ► Inorganic nanomaterials interact with cells to promote physiological processes. ► Nanoelectronic devices are integrated with engineered tissues to monitor function. 相似文献
