共查询到20条相似文献,搜索用时 31 毫秒
1.
《Current opinion in biotechnology》2013,24(6):1116-1121
Highlights► Design of isotopic labeling experiments determines the precision of flux estimates. ► New approaches were developed to select optimal isotopic tracers and measurements. ► Tandem mass spectrometry provides more informative data for 13C flux analysis. ► Parallel labeling experiments can improve the quality of flux estimates. ► New methods are needed for optimal design of parallel labeling experiments. 相似文献
2.
《Current opinion in biotechnology》2013,24(3):516-525
Highlights► Metagenomic microbial community characterization. ► Extreme environment microbiology. ► High and low temperature adaptation. 相似文献
3.
《Current opinion in chemical biology》2013,17(2):143-149
Highlights► Not all chelators are the same: more than metal sequestration and elimination. ► Metal chelates can redistribute metals across biological membranes. ► Metal-chelating fragments provide leads for metalloenzyme inhibition. ► Metal chelation can enhance metal reactivity and thus cytotoxicity. ► Prochelators provide site-activated metal chelation and passivation. 相似文献
4.
《Current opinion in biotechnology》2013,24(4):654-663
Highlights► We focus on the latest developments in nanotechnology-based electrophysiology. ► Methods for extracellular recording via nanoFET-based devices are described. ► Metal nanoelectrodes for the recording of electrogenic cells are reviewed. ► The latest developments of intracellular FET-based probes are shortly covered. ► Pioneering electrical scaffold devices for the interfacing with tissues are described. 相似文献
5.
《Current opinion in biotechnology》2013,24(2):229-238
Highlights► Crop yield depends on efficiency of conversion of light energy into biomass. ► Models have estimated energy conversion efficiency in photosynthesis and metabolism. ► Research is exploring strategies to modify or suppress photorespiration. ► Energy loss during conversion of photosynthate to biomass is also large ► Research is addressing uncertainties over where these losses occur. 相似文献
6.
《Current opinion in biotechnology》2013,24(6):1054-1060
Highlights► Poly(ω-hydroxy fatty acids) are polyethylene-like biobased polyester. ► Medium-chain-length polyhydroxyalkanoate homopolymers are processed to stretchy film. ► Microbial lactate-based polyesters exhibit transparent and flexible properties. ► Enzyme and metabolic engineering facilitates the expanding of bio-based polymers. 相似文献
7.
《Current opinion in biotechnology》2013,24(2):187-191
Highlights► Genome sequencing is an efficient, cost-effective way of characterizing microbes. ► Pangenome analysis gives new insight into texture formation in yoghurt. ► Pangenome analysis of probiotic strains has identified new effect or molecules. ► Pangenome analysis gives insight into probiotic–host specificity. ► Future perspectives include improved strains for food fermentations and probiotics. 相似文献
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9.
《Current opinion in biotechnology》2013,24(2):263-270
Highlights► Transport engineering has the potential to increase nutritional value of crops. ► Transport engineering can increase yield of synthetic biology approaches. ► Neo-functionalization of primary metabolite transporters to specialized metabolism. ► Elimination of glucosinolate seed accumulation is achieved via transport engineering. ► Glucosinolates in Arabidopsis: a model for transport of specialized metabolites. 相似文献
10.
《Current opinion in biotechnology》2013,24(2):291-299
Highlights► Microbial production of pharmaceutical and fuel molecules. ► Systems biology tools advanced our understanding about synthetic pathway design. ► Synthetic biology led us to cell factories tailor-made for chemical production. ► Combinatorial gene assembly for synthetic pathway diversification. ► Engineering static, dynamic and spatial control for better production phenotype. 相似文献
11.
《Current opinion in biotechnology》2013,24(2):214-219
Highlights► The intestinal microbiome is recognized as an important determinant of health. ► Galacto-oligosaccharides are non-digestible carbohydrates. ► Galacto-oligosaccharides stimulate growth and activity of gut bacteria. ► Subtle differences in structure can result in significant differences in activity. ► Less abundant oligosaccharides from bovine milk are similar to those from human milk. 相似文献
12.
《Current opinion in biotechnology》2013,24(1):39-47
Highlights► Genome-wide association studies with metabolomics constitute mGWAS. ► mGWAS provide insights into genetic and environmental impact on metabolic processes. ► We review essential strategies for mGWAS. ► Examples of mGWAS in large cohort studies are discussed. 相似文献
13.
《Current opinion in biotechnology》2013,24(2):135-141
Highlights► This review covers current know-how on cheese flavor development. ► Proteolysis, autolysis and amino acid catabolism by lactic acid bacteria are discussed. ► Systems biology approaches will drive future technology improvements. ► Next-generation sequencing holds promise for fast increase in exploitable knowledge. ► Culture heterogeneity may be significant for industrial flavor production by LAB. 相似文献
14.
《Current opinion in biotechnology》2013,24(2):271-277
Highlights► Recent advances have been made on plant genome-scale metabolic reconstruction. ► Cellular compartmentation makes plant genome-scale reconstruction challenging. ► Current reconstructions capture important features of plant metabolism. ► The models have been used to study isolated tissues and tissue interaction. ► We review the challenges and potential of plant reconstruction and modelling. 相似文献
15.
《Current opinion in biotechnology》2013,24(2):256-262
Highlights► The plastidial pyruvate transporter in plants is driven by sodium symport. ► The mitochondrial pyruvate transporter MPC has been identified. ► All transporters needed for a synthetic PEP-CK/C4 photosynthetic cycle are now known. ► The SWEET proteins define a new class of sugar efflux carriers in plants. 相似文献
16.
《Current opinion in biotechnology》2013,24(4):821-825
Highlights► The action of industrial processes depends on microbial community structure. ► Microbes evolve very rapidly. ► Microbial evolution is affected by community structure. ► Community structure is affected by microbial evolution. ► This feedback may be crucial when considering microbial communities in industry. 相似文献
17.
《Current opinion in biotechnology》2013,24(4):562-574
Highlights► Hybridization chain reactions for amplified DNA detection and DNA assembly. ► Rolling circle amplification-synthesized DNAzyme chains for amplified DNA detection. ► Bi-enzyme cascades and biocatalytic synthesis of metallic nanowires on DNA templates. ► Self-assembled one-dimensional DNA nanostructures for biocatalytic cascades. ► Programmed assembly of metal nanoparticles on one-dimensional DNA nanostructures. 相似文献
18.
《Current opinion in biotechnology》2013,24(2):300-309
Highlights► Triacylglycerol assembly in microalgae might occur at plastid envelopes. ► Starch-less mutants provide clues for the engineering of carbon partitioning in algae. ► New algal species emerge as models for lipid and biofuel research. 相似文献
19.
《Current opinion in biotechnology》2013,24(2):142-147
Highlights► Thiol compounds (glutathione) protect LAB from multiple environmental stresses. ► Engineering antioxidative properties of LAB can improve the overall robustness. ► Genomics approaches helped to reveal the molecular mechanism of stress response. 相似文献
20.
《Current opinion in biotechnology》2013,24(2):354-365
Highlights► Recent metabolic engineering efforts for plant alkaloids. ► Characterizing, reconfiguring and fine-tuning metabolic ‘parts’ improves titers. ► Additional strategies are necessary to produce ‘unnatural’ natural products. 相似文献