Highlights► Engineered feedback loops implement elaborate functionality in synthetic circuits. ► The homeostatic potential of feedback loops is under-utilized in synthetic biology. ► Feedback control of engineered or natural cellular functions can be achieved using biological components or in silico regulation. ► Optogenetics and microfluidic technologies are important for implementing in silico control. 相似文献
Highlights► Microorganisms play a crucial role in detoxifying environmental pollutants. ► Proving that biodegradation processes occur in the real world (in situ) is important. ► Genetics, biochemistry, and physiology advance the science of microbial metabolism. ► Such advances provide new molecular-biomarker tools for documenting biodegradation. ► Applying new tools to contaminated sites reveals new microbial processes and diversity. 相似文献
Highlights► Microbial catalysts with different PCB dechlorinating activities are cultured. ► Biostimulation and bioaugmentation has been successful in the laboratory. ► Molecular tools for monitoring dechlorinating bacteria in situ are available. ► Methods to deploy these catalysts in the field are currently under development. 相似文献
Highlights► Phylogeny of Saccharomyces cerevisiae reveals industry-relevant groupings. ► Comparative genomics reveals genomic variation across industrial yeast strains. ► Next-gen sequencing in next-gen wine yeast strain development. ► Precise mapping of QTLs for marker-assisted breeding of industrial yeast. ► Discovery of putative mobile gene cluster that uses novel type of transposition. 相似文献
Highlights► Magnetic nanoparticles (MNPs) can be in engineered and functionalized. ► MNPs can be used as magnetic resonance imaging contrast agents and targeted MNPs can be used in theranostic applications. ► MNPs are useful for in vivo drug and gene delivery. ► MNPs are useful for photodynamic, phototherapy and hyperthermia. ► MNPs can be used for controlled released and manipulation of cell in vivo. 相似文献
Highlights► This review describes metabolomic studies of anaerobic hydrocarbon-impacted sites. ► The discovery of new pathways has provided new metabolic and molecular biomarkers. ► Assessment of in situ biodegradation is enhanced via integrative approaches. 相似文献
Highlights► The application of Pichia pastoris for biopharmaceutical production is described. ► Synthetic biology approaches and perspectives to improve production are reviewed. ► Glycoengineering efforts to produce humanized, uniform glycoproteins are covered. ► The design and application of synthetic promoter variants are highlighted. ► The molecular toolbox available for synthetic biology in P. pastoris is discussed. 相似文献
Highlights► Stable isotopes provide insights about in situ biodegradation mechanisms and rates. ► Isotopic approaches can be confounded by abiotic processes and heterogeneity effects. ► Isotopic applications are improving with new methods of analysis and modeling. ► Isotopic data are most useful when combined with other approaches for fate assessment. 相似文献
Highlights► Microbial reduction of U(VI) can remove uranium from contaminated groundwater. ► Concerns about the stability of precipitated uranium need further study. ► Environmental omics is useful for diagnosing in situ physiological state of microbes. ► Genome-scale modeling of in situ activity of bioremediation organisms is possible. 相似文献
Highlights► Genome sequencing is an efficient, cost-effective way of characterizing microbes. ► Pangenome analysis gives new insight into texture formation in yoghurt. ► Pangenome analysis of probiotic strains has identified new effect or molecules. ► Pangenome analysis gives insight into probiotic–host specificity. ► Future perspectives include improved strains for food fermentations and probiotics. 相似文献
Highlights► Crop yield depends on efficiency of conversion of light energy into biomass. ► Models have estimated energy conversion efficiency in photosynthesis and metabolism. ► Research is exploring strategies to modify or suppress photorespiration. ► Energy loss during conversion of photosynthate to biomass is also large ► Research is addressing uncertainties over where these losses occur. 相似文献
Highlights► RiPPs have high capabilities of target interaction far beyond the field of anti-infectives. ► RiPPs are especially suitable for design and engineering with the genetic code expansion. ► First published studies yielded RiPPs with unnatural/synthetic amino acids. ► Achievement: expanded scope of the in vivo (mainly bacterial) RiPPs synthesis. ► Perspective: design of RiPPS with novel chemistries and unique sequence combinations. 相似文献
Highlights► Individual DNA molecules hundreds of kbp long may be stretched and visualized by optical microscopy. ► An optical barcode is generated by fluorescent labeling of short sequence motifs along the stretched DNA. ► Optical maps complement DNA sequencing for gap closing, finishing, validation and de novo assembly of genomes. ► Genome structural variations not accessible to sequencing or DNA arrays may be directly visualized. ► Epigenetic marks such as DNA methylation and DNA binding proteins may also be mapped on single genomic fragments. 相似文献
Highlights► The exposure of bacteria to pollutants induces chemoattraction or chemorepellence. ► Pollutant chemoreceptors so far identified are present on plasmids. ► Chemotaxis increases pollutant bioavailability and enhances biodegradation rates. ► Chemotaxis may improve microbial inoculants designed just on catabolic potential. 相似文献
Highlights► The irreversible inactivation of protein targets by metal complexes is described. ► The article summaries catalysts that hydrolyze or oxidize proteins. ► Cyclen-based catalysts that selectively hydrolyze peptides and proteins are described. ► Catalysts that generate reactive oxygen species are important for protein inactivation. ► Metal-based oxidants are potent and selective agents for protein inactivation. 相似文献
Highlights► Responsive MRI contrast agents enable the study of biochemical events. ► Recent Gd-based contrast agents are reviewed here. ► Responsive agents act by modulating hydration state, molecular tumbling or number of metal centres. ► Promising strategies for future probe design are identified. 相似文献
Highlights► Flux control does not reside in individual glycolytic enzymes in L. lactis. ► ATP demand does not control the glycolytic flux in L. lactis. ► Lactic acid bacteria are excellent candidates for the cell factories of the future. 相似文献
