共查询到20条相似文献,搜索用时 31 毫秒
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Cian J. Lynch Raquel Bernad Isabel Calvo Sandrina Nóbrega-Pereira Sergio Ruiz Nuria Ibarz Ana Martinez-Val Osvaldo Graña-Castro Gonzalo Gómez-López Eduardo Andrés-León Vladimir Espinosa Angarica Antonio del Sol Sagrario Ortega Oscar Fernandez-Capetillo Enrique Rojo Javier Munoz Manuel Serrano 《Cell reports》2018,22(2):396-410
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Changkeun Lee Anne S. Tibbetts Gisela Kramer Dean R. Appling 《The Journal of biological chemistry》2009,284(49):34116-34125
Initiation of protein synthesis in mitochondria and chloroplasts normally uses a formylated initiator methionyl-tRNA (fMet-tRNAfMet). However, mitochondrial protein synthesis in Saccharomyces cerevisiae can initiate with nonformylated Met-tRNAfMet, as demonstrated in yeast mutants in which the nuclear gene encoding mitochondrial methionyl-tRNA formyltransferase (FMT1) has been deleted. The role of formylation of the initiator tRNA is not known, but in vitro formylation increases binding of Met-tRNAfMet to translation initiation factor 2 (IF2). We hypothesize the existence of an accessory factor that assists mitochondrial IF2 (mIF2) in utilizing unformylated Met-tRNAfMet. This accessory factor might be unnecessary when formylated Met-tRNAfMet is present but becomes essential when only the unformylated species are available. Using a synthetic petite genetic screen in yeast, we identified a mutation in the AEP3 gene that caused a synthetic respiratory-defective phenotype together with Δfmt1. The same aep3 mutation also caused a synthetic respiratory defect in cells lacking formylated Met-tRNAfMet due to loss of the MIS1 gene that encodes the mitochondrial C1-tetrahydrofolate synthase. The AEP3 gene encodes a peripheral mitochondrial inner membrane protein that stabilizes mitochondrially encoded ATP6/8 mRNA. Here we show that the AEP3 protein (Aep3p) physically interacts with yeast mIF2 both in vitro and in vivo and promotes the binding of unformylated initiator tRNA to yeast mIF2. We propose that Aep3p functions as an accessory initiation factor in mitochondrial protein synthesis. 相似文献
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