共查询到20条相似文献,搜索用时 0 毫秒
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A report on the XXXV International Congress of Physiological Sciences, held together with Experimental Biology 2005, San Diego, USA, 31 March - 6 April 2005. 相似文献
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Hope IA 《Trends in genetics : TIG》2001,17(6):297-299
Four recent papers mark a major shift in functional genomic analysis for multicellular organisms. RNA-mediated interference was applied to inactivate individual genes systematically on a genomic scale. These studies subjected a third of the genes in the genome of Caenorhabditis elegans to reverse genetic analysis. 相似文献
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MaizeDB - a functional genomics perspective 总被引:1,自引:0,他引:1
Polacco M Coe E Fang Z Hancock D Sanchez-Villeda H Schroeder S 《Comparative and Functional Genomics》2002,3(2):128-131
MaizeDB (http://www.agron.missouri.edu/) has existed since the early 90's as a genomespecific database that is grounded in genetic maps, their documentation and annotation. The database management system is robust and has continuously been Sybase. In this brief review we provide an introduction to the database as a functional genomics tool and new accesses to the data: 1) probe tables by bin location 2) BLAST access to map data 3) cMap, a comparative map graphical tool. 相似文献
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Kurowska M Daszkowska-Golec A Gruszka D Marzec M Szurman M Szarejko I Maluszynski M 《Journal of applied genetics》2011,52(4):371-390
Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional
mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN
Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis,
sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage
of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy
level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic
potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations
reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200–500 kb
screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the
rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING
public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic
tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives
for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The
TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput
method in basic and applied research have already been implemented through modifications of the original TILLING strategy,
such as Ecotilling or Deletion TILLING. 相似文献
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Bulyk ML 《Genome biology》2004,5(7):331
A report on the Keystone Symposium 'Biological Discovery Using Diverse High-Throughput Data', Steamboat Springs, USA, 30 March-4 April 2004. 相似文献
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近十年来,生理学与基因组学达到了空前的融合。尽管生理基因组学还是一个非常年轻的研究领域,系统生物学概念的引入必将推进生理基因组学达到全新的水平。本文概要地叙述了这个令人振奋的生理科学的新时代给生理学家带来的机遇和挑战,并以我们自己近十年来的经验为例,讨论了怎样通过扩展和延伸生理学与基因组学的结合,从而对生物学得到系统的理解。 相似文献
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Aptamers come of age - at last 总被引:5,自引:0,他引:5
Nucleic-acid aptamers have the molecular recognition properties of antibodies, and can be isolated robotically for high-throughput applications in diagnostics, research and therapeutics. Unlike antibodies, however, they can be chemically derivatized easily to extend their lifetimes in biological fluids and their bioavailability in animals. The first aptamer-based clinical drugs have recently entered service. Meanwhile, active research programmes have identified a wide range of anti-viral aptamers that could form the basis for future therapeutics. 相似文献
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Kyuyoung Song 《Biotechnology and Bioprocess Engineering》2000,5(5):307-312
As the first assembly of the human genome was announced on June 26, 2000, we have entered post genome era. The genome sequence
represents a new starting point for science and medicine with possible impact on research across the life sciences. In this
review I tried to offer brief summaries of history and progress of the Human Genome Project and two major challenges ahead,
functional genomics and DNA sequence variation research. 相似文献
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With the complete sequencing of the human genome, research priorities have shifted from the identification of genes to the elucidation of their function. Methods currently used by scientists to characterize gene function, such as knock-out mice, are based upon loss of protein function and analysis of the resulting phenotypes to infer a potential role for the protein under scrutiny. Until now, these methods have been successful but time consuming and only a few genes at a time could be analyzed. Cell microarrays allow to simultaneously transfect thousands of different nucleic acid molecules, RNA or DNA, into adherent cells. It is then possible to analyze a large pallet of resulting phenotypes in clusters of transfected cells. We are currently manufacturing cell microarrays with collections of full-length cDNA cloned in expression vectors (gain of function analyses) or siRNA (loss of function studies) to unravel function of genes involved in differentiation and proliferation of human cells. Although there are still some technological difficulties to overcome, the potential for cell microarrays to speed up functional exploration of genomes is very promising. 相似文献
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A unique combination of disciplines is emerging--evolutionary and ecological functional genomics--which focuses on the genes that affect ecological success and evolutionary fitness in natural environments and populations. Already this approach has provided new insights that were not available from its disciplinary components in isolation. However, future advances will necessitate the re-engineering of scientific attitudes, training and institutions, to achieve extensive multidisciplinarity. 相似文献
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功能基因组学的研究方法 总被引:9,自引:1,他引:9
基因组学的研究已从结构基因组学转向功能基因组学,功能基因组学时代对于基因功能的研究也由单一基因转向大规模,批量分析,本综述了功能基因组学的研究内容与方法,主要包括:差异显示反转录PCR,基因表达序列分析(SAGE),微点阵,遗传足迹法,反求遗传学,蛋白质组学和生物信息学等新方法。 相似文献
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RNAi for plant functional genomics 总被引:9,自引:0,他引:9
Matthew L 《Comparative and Functional Genomics》2004,5(3):240-244
A major challenge in the post-genome era of plant biology is to determine the functions of all the genes in the plant genome. A straightforward approach to this problem is to reduce or knock out expression of a gene with the hope of seeing a phenotype that is suggestive of its function. Insertional mutagenesis is a useful tool for this type of study, but it is limited by gene redundancy, lethal knock-outs, nontagged mutants and the inability to target the inserted element to a specific gene. RNA interference (RNAi) of plant genes, using constructs encoding self-complementary 'hairpin' RNA, largely overcomes these problems. RNAi has been used very effectively in Caenorhabditis elegans functional genomics, and resources are currently being developed for the application of RNAi to high-throughput plant functional genomics. 相似文献