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1.
The exodermis of Iris germanica roots is multiseriate. Its outermost layer matures first with typical Casparian bands and suberin lamellae. But as subsequent layers mature, the Casparian band extends into the tangential and anticlinal walls of their cells. Compared with roots in which the endodermis represents the major transport barrier, the multiseriate exodermis (MEX) was expected to reduce markedly radial water and solute transport. To test this idea, precocious maturation of the exodermis was induced with a humid air gap inside a hydroponic chamber. Hydraulic conductivity (Lp(pc)) was measured on completely submerged roots (with an immature exodermis) and on air-gap-exposed root regions (with two mature exodermal layers) using a pressure chamber. Compared with regions of roots with no mature exodermal layers, the mature MEX reduced Lp(pc) from 8.5×10(-8) to 3.9×10(-8) m s(-1) MPa(-1). Puncturing the MEX increased Lp(pc) to 19×10(-8) m s(-1) MPa(-1), indicating that this layer constituted a substantial hydraulic resistance within the root (75% of the total). Alternatively, a root pressure probe was used to produce pressure transients from which hydraulic conductivity was determined, but this device measured mainly flow through the endodermis in these wide-diameter roots. The permeability of roots to NaCl and ethanol was also reduced in the presence of two mature MEX layers. The data are discussed in terms of the validity of current root models and in terms of a potential role for I. germanica MEX during conditions of drought and salt stress. 相似文献
2.
Anders Sonesson Hermann Moll Erik Jantzen Ulrich Zähringer 《FEMS microbiology letters》1993,106(3):315-320
Abstract Four long-chain fatty acids, 2-hydroxy-27-oxo-octacosanoic acid ( n 28:0(2-OH,27-oxo)), 2-hydroxy-29-oxo-triacontanoic acid ( n 30:0(2-OH,29-oxo)), 2-hydroxy-heptacosane-1,27-dioic acid (27:0(2-OH)-dioic) and 2-hydroxy-nonacosane-1,29-dioic acid (29:0(2-OH)-dioic) were identified by GLC-MS analysis in the phenol-chloroform-petroleum ether (PCP) extracts of Legionella jordanis, L. maceachernii and L. micdadei indicating that they are constituents of lipopolysaccharide. Moreover, five long-chain fatty acids (28:0(27-OH), 28:0(27-oxo), 30:0(29-oxo), 27:0-dioic and 29:0-dioic) previously identified in L. pneumophila (Moll, H. et al., FEMS Microbiol. Lett., 97 (1992), 1–6) were also found in these species. This is to our knowledge the first report on the existence of long chain 2-hydroxylated (ω-1)-oxo fatty acids and 2-hydroxylated 1,ω-dioic fatty acids. 相似文献
3.
Background and Aims
Most studies of exodermal structure and function have involved species with a uniseriate exodermis. To extend this work, the development and apoplastic permeability of Iris germanica roots with a multiseriate exodermis (MEX) were investigated. The effects of different growth conditions on MEX maturation were also tested. In addition, the exodermises of eight Iris species were observed to determine if their mature anatomy correlated with habitat.Methods
Plants were grown in soil, hydroponics (with and without a humid air gap) or aeroponics. Roots were sectioned and stained with various dyes to detect MEX development from the root apical meristem, Casparian bands, suberin lamellae and tertiary wall thickenings. Apoplastic permeability was tested using dye (berberine) and ionic (ferric) tracers.Key Results
The root apical meristem was open and MEX development non-uniform. In soil-grown roots, the exodermis started maturing (i.e. Casparian bands and suberin lamellae were deposited) 10 mm from the tip, and two layers had matured by 70 mm. In both hydro- and aeroponically grown roots, exodermal maturation was delayed. However, in areas of roots exposed to an air gap in the hydroponic system, MEX maturation was accelerated. In contrast, maturation of the endodermis was not influenced by the growth conditions. The mature MEX had an atypical Casparian band that was continuous around the root circumference. The MEX prevented the influx and efflux of berberine, but had variable resistance to ferric ions due to their toxic effects. Iris species living in well-drained soils developed a MEX, but species in water-saturated substrates had a uniseriate exodermis and aerenchyma.Conclusions
MEX maturation was influenced by the roots'' growth medium. The MEX matures very close to the root tip in soil, but much further from the tip in hydro- and aeroponic culture. The air gap accelerated maturation of the second exodermal layer. In Iris, the type of exodermis was correlated with natural habitat suggesting that a MEX may be advantageous for drought tolerance.Key words: Iris germanica, roots, culture conditions, development, anatomy, apoplastic tracers, multiseriate exodermis, endodermis, root apical meristem 相似文献4.
Hydroxycinnamates in suberin formation 总被引:1,自引:0,他引:1
José Graça 《Phytochemistry Reviews》2010,9(1):85-91
Hydroxycinnamates are found associated with suberin in several forms: covalently linked to the aliphatic suberin; in the residue
after suberin-removal; and in the non-polar extractives of suberized tissues in the form of alkyl ferulates. Suberin-associated
hydroxycinnamates have been found mainly as ferulic acid-derivatives, sometimes as feruloylamides and in a lesser extent as
caffeates. Ferulic acid esters of long-chain ω-hydroxyacids are prevalent in the partial depolymerisation products of suberin.
Also, enzymes able to catalyze the feruloylation of ω-hydroxyacids were found timely-associated with the suberization process.
It is proposed that ferulic acid, and its dimers, through esterification to ω-hydroxyacids, covalently link the suberin aliphatic
polyester to suberin-associated polyaromatics. In this case, the known role of ferulates, and related hydroxycinnamates, as
cross-linkers of structurally different polymers would be enlarged to suberized cell-walls. 相似文献
5.
Schreiber L Franke R Hartmann KD Ranathunge K Steudle E 《Journal of experimental botany》2005,56(415):1427-1436
Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degradation (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were analysed quantitatively by gas chromatography and mass spectrometry. In corn, suberin was determined for isolated endodermal (ECW) and rhizo-hypodermal (RHCW) cell walls. In rice, the strong lignification of the central cylinder (CC), did not allow the isolation of endodermal cell walls. Similarly, exodermal walls could not be separated from the rhizodermal and sclerenchyma cell layers. Suberin analyses of ECW and RHCW of rice, thus, refer to either the entire CC or to the entire outer part of the root (OPR), the latter lacking the inner cortical cell layer. In both species, aromatic suberin was mainly composed of coumaric and ferulic acids. Aliphatic suberin monomers released from rice and corn belonged to five substance classes: primary fatty acids, primary alcohols, diacids, omega-hydroxy fatty acids, and 2-hydroxy fatty acids, with omega-hydroxy fatty acids being the most prominent substance class. Qualitative composition of aliphatic suberin of rice was different from that of corn; (i) it was much less diverse, and (ii) besides monomers with chain lengths of C(16), a second maximum of C(28) was evident. In corn, C(24) monomers represented the most prominent class of chain lengths. When suberin quantities were related to surface areas of the respective tissues of interest (hypodermis and/or exodermis and endodermis), exodermal cell walls of rice contained, on average, six-times more aliphatic suberin than those of corn. In endodermal cell walls, amounts were 34 times greater in rice than in corn. Significantly higher amounts of suberin detected in the apoplastic barriers of rice corresponded with a substantially lower root hydraulic conductivity (Lp(r)) compared with corn, when water flow was driven by hydrostatic pressure gradients across the apoplast. As the OPR of rice is highly porous and permeable to water, it is argued that this holds true only for the endodermis. The results imply that some caution is required when discussing the role of suberin in terms of an efficient transport barrier for water. The simple view that only the quantity of suberin present is important, may not hold. A more detailed consideration of both the chemical nature of suberins and of the microstructure of deposits is required, i.e. how suberins impregnate wall pores. 相似文献
6.
Castor bean (Ricinus communis L.) plants were hydroponically cultivated to achieve NO3 deficiency (N starvation), salt stress (addition of 100 mM NaCl), or normal conditions. Endodermal (ECW) and rhizodermal
and hypodermal cell walls (RHCW) were isolated enzymatically from roots, and suberin monomers were released by transesterification
after solvent extraction. Aromatic and aliphatic suberin monomers were identified and quantified by gas chromatography and
mass spectrometry. Between 90 and 95% of the released suberin monomers were linear, long-chain, aliphatic compounds (alcohols,
acids, diacids, ω-hydroxy acids and 2-hydroxy acids) with an average chain length of 19 C-atoms. The remainder was an aromatic
suberin fraction mainly composed of coumaric and ferulic acid. Suberin amounts were significantly increased in ECW and RHCW
in the presence of NaCl. In contrast, N starvation led to significantly reduced levels of suberization in ECW and RHCW. It
is concluded that R. communis plants reinforce their apoplastic transport barriers in roots in adaptation to NaCl stress in order to minimize NaCl uptake.
Under conditions of N starvation the opposite occurs and plants reduce the suberization of their apoplastic transport barriers
to facilitate nutrient uptake form the soil. 相似文献
7.
Determination of structure and composition of suberin from the roots of carrot, parsnip, rutabaga, turnip, red beet, and sweet potato by combined gas-liquid chromatography and mass spectrometry 总被引:7,自引:7,他引:0
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Suberin from the roots of carrots (Daucus carota), parsnip (Pastinaca sativa), rutabaga (Brassica napobrassica), turnip (Brassica rapa), red beet (Beta vulgaris), and sweet potato (Ipomoea batatas) was isolated by a combination of chemical and enzymatic techniques. Finely powdered suberin was depolymerized with 14% BF3 in methanol, and soluble monomers (20-50% of suberin) were fractionated into phenolic (<10%) and aliphatic (13-35%) fractions. The aliphatic fractions consisted mainly of ω-hydroxyacids (29-43%), dicarboxylic acids (16-27%), fatty acids (4-18%), and fatty alcohols (3-6%). Each fraction was subjected to combined gas-liquid chromatography and mass spectrometry. Among the fatty acids very long chain acids (>C20) were the dominant components in all six plants. In the alcohol fraction C18, C20, C22, and C24 saturated primary alcohols were the major components. C16 and C18 dicarboxylic acids were the major dicarboxylic acids of the suberin of all six plants and in all cases octadec-9-ene-1, 18-dioic acid was the major component except in rutabaga where hexadecane-1, 16-dioic acid was the major dicarboxylic acid. The composition of the ω-hydroxyacid fraction was quite similar to that of the dicarboxylic acids; 18-hydroxy-octadec-9-enoic acid was the major component in all plants except rutabaga, where equal quantities of 16-hydroxyhexadecanoic acid and 18-hydroxyoctadec-9-enoic acid (42% each) were found. Compounds which would be derived from 18-hydroxyoctadec-9-enoic acid and octadec-9-ene-1, 18-dioic acid by epoxidation, and epoxidation followed by hydration of the epoxide, were also detected in most of the suberin samples. The monomer composition of the six plants showed general similarities but quite clear taxonomic differences. 相似文献
8.
The fibers of the green lint mutant of cotton (Gossypium hirsutum L.) contain large amounts of wax and are suberized. More than 96% of the bifunctional aliphatic suberin monomers ([alpha],[omega]-alkanedioic acids and [omega]-hydroxyalkanoic acids) have chain lengths of C22 and C24 in green cotton fiber suberin. In fibers grown in the presence of S-ethyl-N,N-dipropylthiocarbamate (EPTC), a specific inhibitor of the endoplasmic reticulum-associated fatty acid elongases, the aliphatic suberin monomers were shortened to chain lengths of C16 and C18. Whereas the amounts of most suberin monomers were not negatively affected by the inhibitor treatment, the amounts of [alpha],[omega]-alkanedioic acids and of glycerol were reduced by more than 80%. Analysis in the transmission electron microscope showed a reduction in suberin content after EPTC treatment. The suberin layers were discontinuous and consisted of fewer lamellae than in the controls. A small proportion (up to 22%) of the electron-translucent suberin lamellae were thinner after EPTC treatment, probably because of the shortening of the aliphatic suberin monomers. A larger proportion of the electron-translucent lamellae were thicker than the lamellae in the controls. Possible explanations for this observation are discussed. 相似文献
9.
Effects of exposure to humid air on epidermal viability and suberin deposition in maize (Zea mays L.) roots 总被引:4,自引:1,他引:3
When the basal zones of 4-d-old hydroponically grown maize ( Zea mays L. cv. Seneca Horizon) roots were exposed to moist air for 2 d, the development of both endodermis and exodermis was affected. In the endodermis, Casparian bands enlarged and more cells developed suberin lamellae. The most striking effect was seen in the exodermis. In submerged controls, only 4% of the cells had Casparian bands, whereas in root regions exposed to air, 93% developed these structures. Similarly, in submerged roots 11% of the exodermal cells had either developing or mature suberin lamellae compared with 92% in the air-treated region. The majority of epidermal cells remained alive in the zone exposed to air. Some cell death had occurred earlier in the experiment when the seedlings were transferred from vermiculite to hydroponic culture. The precise stimulus(i) associated with the air treatment which led to accelerated development in both endodermis and exodermis is as yet unknown. 相似文献
10.
P. Olesen 《Protoplasma》1978,94(3-4):325-340
Summary InHoya roots most exodermal cells are elongated and a band of suberin lamellae is formed in all their walls early in development; later on carbohydrate tertiary wall layers are deposited inside the suberin lamellae. Some exodermal cells which are restricted to root hair-bearing areas are short and unsuberized but their outer tangential wall is conspicuously thickened. Combined evidence from light microscopy and transmission and scanning electron microscopy reveals the bulk of this cap-formed thickening as a mosaic structure with two different components forming an extensive labyrinth. Irregular masses of a lignified, amorphous substance are separated by radially oriented, tortuous channels containing a very dense, granular-fibrillar material. The innermost wall layer is fibrillar and shows a texture and density similar to the material in the separating channels. The cap contains prominent pits with plasmodesmatal connections between short cells and the epidermis. In mature and non-functional short cells a band of suberin lamellae and eventually tertiary wall layers are deposited.A hypothesis as to the function of the short cells is based on the assumption that the cap functions through differential shrinkage of two components forming the labyrinthine structure. This would ensure a very effective closing of the translocating pathway upon desiccation and shrinkage and a consequent swelling and re-opening upon rehydration. The regulatory function of such mechanism is discussed. 相似文献
11.
Suberin, a cell specific, wall-associated biopolymer, is formed during normal plant growth and development as well as in response to stress conditions such as wounding. It is characterized by the deposition of both a poly(phenolic) domain (SPPD) in the cell wall and a poly(aliphatic) domain (SPAD) thought to be deposited between the cell wall and plasma membrane. Although the monomeric components that comprise the SPPD and SPAD are well known, the biosynthesis and deposition of suberin is poorly understood. Using wound healing potato tubers as a model system, we have tracked the flux of carbon into the aliphatic monomers of the SPAD in a time course fashion. From these analyses, we demonstrate that newly formed fatty acids undergo one of two main metabolic fates during wound-induced suberization: (1) desaturation followed by oxidation to form the 18:1 ω-hydroxy and dioic acids characteristic of potato suberin, and (2) elongation to very long chain fatty acids (C20 to C28), associated with reduction to 1-alkanols, decarboxylation to n-alkanes and minor amounts of hydroxylation. The partitioning of carbon between these two metabolic fates illustrates metabolic regulation during wound healing, and provides insight into the organization of fatty acid metabolism.Key Words: suberin, potato, Solanum tuberosum, carbon flux analysis, abiotic stress 相似文献
12.
Katsuhiro Shiono Miho Ando Shunsaku Nishiuchi Hirokazu Takahashi Kohtaro Watanabe Motoaki Nakamura Yuichi Matsuo Naoko Yasuno Utako Yamanouchi Masaru Fujimoto Hideki Takanashi Kosala Ranathunge Rochus B. Franke Nobukazu Shitan Naoko K. Nishizawa Itsuro Takamure Masahiro Yano Nobuhiro Tsutsumi Lukas Schreiber Kazufumi Yazaki Mikio Nakazono Kiyoaki Kato 《The Plant journal : for cell and molecular biology》2014,80(1):40-51
Suberin is a complex polymer composed of aliphatic and phenolic compounds. It is a constituent of apoplastic plant interfaces. In many plant species, including rice (Oryza sativa), the hypodermis in the outer part of roots forms a suberized cell wall (the Casparian strip and/or suberin lamellae), which inhibits the flow of water and ions and protects against pathogens. To date, there is no genetic evidence that suberin forms an apoplastic transport barrier in the hypodermis. We discovered that a rice reduced culm number1 (rcn1) mutant could not develop roots longer than 100 mm in waterlogged soil. The mutated gene encoded an ATP‐binding cassette (ABC) transporter named RCN1/OsABCG5. RCN1/OsABCG5 gene expression in the wild type was increased in most hypodermal and some endodermal roots cells under stagnant deoxygenated conditions. A GFP‐RCN1/OsABCG5 fusion protein localized at the plasma membrane of the wild type. Under stagnant deoxygenated conditions, well suberized hypodermis developed in wild types but not in rcn1 mutants. Under stagnant deoxygenated conditions, apoplastic tracers (periodic acid and berberine) were blocked at the hypodermis in the wild type but not in rcn1, indicating that the apoplastic barrier in the mutant was impaired. The amount of the major aliphatic suberin monomers originating from C28 and C30 fatty acids or ω‐OH fatty acids was much lower in rcn1 than in the wild type. These findings suggest that RCN1/OsABCG5 has a role in the suberization of the hypodermis of rice roots, which contributes to formation of the apoplastic barrier. 相似文献
13.
Suberin lamella development in maize seedling roots grown in aerated and stagnant conditions 总被引:4,自引:0,他引:4
Hypoxia can stimulate the development of a suberized exodermis in aquatic plants; however, its influence on this aspect of terrestrial root development is sparsely documented. To determine the effects of hypoxia on maize (Zea mays cv. Seneca Horizon) roots, seedlings were grown in vermiculite (VERM), aerated hydroponics (AER), stagnant hydroponics with agar (STAG), or aerated hydroponics with agar (AERAG). The endo- and exodermis were examined for wall modifications. Lateral root emergence and aerenchyma formation were documented qualitatively. The endodermal Casparian band formation was unaffected by treatment. Endodermal and exodermal suberin lamella formation was earliest and most extensive in VERM. Suberization, especially in the exodermis of aerated treatments, was depressed in all hydroponic media. In comparison with AER, STAG exodermal lamellae were increased, but endodermal lamellae were decreased. Since the suberized exodermis forms a barrier to radial oxygen loss from roots to the medium, its stimulation in STAG roots (which also developed extensive aerenchyma) would help retain oxygen in the root. The reduction of endodermal lamellae should facilitate oxygen diffusion into the stele. Clearly, the response to environmental conditions is variable within individual cortical cell layers. Additionally, the observed patterns of lamellae, aerenchyma and lateral root development indicate a tight radial co-ordination of root development. 相似文献
14.
Mature seeds of Arabidopsis thaliana and Brassica napus contain a complex mixture of aliphatic monomers derived from the non-extractable lipid polyesters deposited by various seed tissues. Methods of polyester depolymerization of solvent-extracted seeds and analysis of aliphatic monomers were compared. Sodium methoxide-catalyzed depolymerization, followed by GC analysis of the acetylated monomers, was developed for routine quantitative analysis suitable for 0.5g seed samples. In Arabidopsis seeds, the major C16 and C18 monomers identified included omega-hydroxy fatty acids and alpha,omega-dicarboxylic acids derived from palmitate, oleate and linoleate, and 9,10,18-trihydroxyoctadecenoic acid. Among monomers which can collectively be considered likely to be derived from suberin, docosan-1-ol, docosane-1,22-diol, 22-hydroxydocosanoic acid, 24-hydroxytetracosanoic acid, tetracosane-1,24-dioic acid and ferulic acid were the major species. Compared to Arabidopsis, Brassica seeds showed a roughly similar proportion of monomer classes, with the exception that alkan-1ols were 3-fold higher. Also, there were much less C24 aliphatic species and significant amounts of C14-C16 alkan-1ols, including iso- and anteiso-methyl branched compounds. Dissection and analysis of mature Brassica seeds showed that the trihydroxy C18:1 fatty acid was found mainly in the embryo, while ferulate, fatty alcohols and C22 and C24 species were specific to the seed coat plus endosperm. 相似文献
15.
Composition of suberin-associated waxes from the subterranean storage organs of seven plants 总被引:4,自引:0,他引:4
The waxes associated with the suberin in the periderm of the underground storage organs of parsnip (Pastinaca sativa L.), carrot (Daucus carota L.), rutabaga (Brassica napobrassica Mill.), turnip (Brassica rapa L.), red beet (Beta vulgaris L.), sweet potato (Ipomoea batatas L.) and potato (Solanum tuberosum L.) were isolated, fractionated into hydrocarbon, wax ester, free fatty alcohol and free fatty acid fractions, and analyzed
by combined gas chromatography and mass spectrometry. The amount of wax extracted from the periderm of the storage organs
ranged from 2 to 32 μg/cm2. The hydrocarbons from the suberin layer have a broader chain-length distribution, a predominance of shorter carbon chains,
and a higher proportion of even-numbered carbon chains than the leaf alkanes from the same plants. The major components of
the free and esterified fatty alcohols and fatty acids have an even number of carbon atoms, and are similar in chain-length
distribution to their counterparts found covalently attached to the suberin polymers; however, these suberin components are
shorter in chain length than their cuticular analogues from the leaves. Also extracted from the storage organs were polar
components which included fatty alcohols and fatty acids in a conjugated form, and ω-hydroxy acids and dicarboxylic acids.
Evidence is presented that removal of the wax from the periderm of whole storage organs results in a decrease in diffusion
resistance to moisture.
Scientific Paper No. 5516, Project 2001, College of Agriculture Research Center, Washington State University, Pullman, WA
99164, USA 相似文献
16.
Yanni Zhang Alexandra Kachura Anica Bjelica M. Alejandra Rey Rincon Karina M. Kaberi Mark A. Bernards 《The Plant journal : for cell and molecular biology》2018,93(5):931-942
Wound‐induced suberin deposition involves the temporal and spatial coordination of phenolic and fatty acid metabolism. Phenolic metabolism leads to both soluble metabolites that accumulate as defense compounds as well as hydroxycinnamoyl derivatives that form the basis of the poly(phenolic) domain found in suberized tissue. Fatty acid metabolism involves the biosynthesis of very‐long‐chain fatty acids, 1‐alkanols, ω‐hydroxy fatty acids and α,ω‐dioic acids that form a poly(aliphatic) domain, commonly referred to as suberin. Using the abscisic acid (ABA) biosynthesis inhibitor fluridone (FD), we reduced wound‐induced de novo biosynthesis of ABA in potato tubers, and measured the impact on the expression of genes involved in phenolic metabolism (StPAL1, StC4H, StCCR, StTHT), aliphatic metabolism (StCYP86A33, StCYP86B12, StFAR3, StKCS6), metabolism linking phenolics and aliphatics (StFHT) or acyl chains and glycerol (StGPAT5, StGPAT6), and in the delivery of aliphatic monomers to the site of suberization (StABCG1). In FD‐treated tissue, both aliphatic gene expression and accumulation of aliphatic suberin monomers were delayed. Exogenous ABA restored normal aliphatic suberin deposition in FD‐treated tissue, and enhanced aliphatic gene expression and poly(aliphatic) domain deposition when applied alone. By contrast, phenolic metabolism genes were not affected by FD treatment, while FD + ABA and ABA treatments slightly enhanced the accumulation of polar metabolites. These data support a role for ABA in the differential induction of phenolic and aliphatic metabolism during wound‐induced suberization in potato. 相似文献
17.
Olga Serra Carolin Hohn Rochus Franke Salomé Prat Marisa Molinas Mercè Figueras 《The Plant journal : for cell and molecular biology》2010,62(2):277-290
Suberin and waxes embedded in the suberin polymer are key compounds in the control of transpiration in the tuber periderm of potato (Solanum tuberosum). Suberin is a cell‐wall biopolymer with aliphatic and aromatic domains. The aliphatic suberin consists of a fatty acid polyester with esterified ferulic acid, which is thought to play an important role in cross‐linking to the aromatic domain. In potato, ferulic acid esters are also the main components of periderm wax. How these ferulate esters contribute to the periderm water barrier remains unknown. Here we report on a potato gene encoding a fatty ω‐hydroxyacid/fatty alcohol hydroxycinnamoyl transferase (FHT), and study its molecular and physiological relevance in the tuber periderm by means of a reverse genetic approach. In FHT RNAi periderm, the suberin and its associated wax contained much smaller amounts of ferulate esters, in agreement with the in vitro ability of the FHT enzyme to conjugate ferulic acid with ω‐hydroxyacid and fatty alcohols. FHT down‐regulation did not affect the typical suberin lamellar ultrastructure but had significant effects on the anatomy, sealing properties and maturation of the periderm. The tuber skin became thicker and russeted, water loss was greatly increased, and maturation was prevented. FHT deficiency also induced accumulation of the hydroxycinnamic acid amides feruloyl and caffeoyl putrescine in the periderm. We discuss these results in relation to the role attributed to ferulates in suberin molecular architecture and periderm impermeability. 相似文献
18.
Comparative study about the salt-induced oxidative stress and lipid composition has been realised in primary root tissues
for two varieties of maize (Zea mays L.) in order to evaluate their responses to salt stress. The root growth, root water content (WC), hydrogen peroxide (H2O2) generation, lipid peroxidation, membrane stability index and the changes in the profile of fatty acids composition were
investigated. Salinity impacts in term of root growth, water content, H2O2 generation, lipid peroxidation and membrane destabilisation were more pronounced in primary roots of Aristo than in those of Arper indicating more sensitivity of the first variety. It was confirmed by gas chromatography that the composition of fatty acids
in roots of both varieties was constituted mainly by 16:0 and 18:0 as major saturated fatty acids and 18:1ω9, 18:2ω6 and 18:3ω3
as major unsaturated fatty acids. Total lipid extracts from the roots of both varieties showed that the lipid saturation level
increased under salt stress, notwithstanding the increased proportion of polyunsaturated fatty acids. The changes in lipid
saturation being predominantly due to decreases in oleic acid (18:1ω9) and increases in palmitic acid (16:0). However, Arper root extracts contained a lower proportion of saturated lipids than Aristo. The enhanced proportion of highly polyunsaturated fatty acids especially linolenic and eicosapentaenoic acids was considered
to be the characteristic of the relatively salt tolerance in Arper roots. 相似文献
19.
Sollapura J. Vishwanath Dylan K. Kosma Ian P. Pulsifer Sabine Scandola Stéphanie Pascal Jér?me Joubès Franziska Dittrich-Domergue René Lessire Owen Rowland Frédéric Domergue 《Plant physiology》2013,163(3):1118-1132
Suberin is found in a variety of tissues, such as root endoderms and periderms, storage tuber periderms, tree cork layer, and seed coats. It acts as a hydrophobic barrier to control the movement of water, gases, and solutes as well as an antimicrobial barrier. Suberin consists of polymerized phenolics, glycerol, and a variety of fatty acid derivatives, including primary fatty alcohols. We have conducted an in-depth analysis of the distribution of the C18:0 to C22:0 fatty alcohols in Arabidopsis (Arabidopsis thaliana) roots and found that only 20% are part of the root suberin polymer, together representing about 5% of its aliphatic monomer composition, while the remaining 80% are found in the nonpolymeric (soluble) fraction. Down-regulation of Arabidopsis FATTY ACYL REDUCTASE1 (FAR1), FAR4, and FAR5, which collectively produce the fatty alcohols found in suberin, reduced their levels by 70% to 80% in (1) the polymeric and nonpolymeric fractions from roots of tissue culture-grown plants, (2) the suberin-associated root waxes from 7-week-old soil-grown plants, and (3) the seed coat suberin polymer. By contrast, the other main monomers of suberin were not altered, indicating that reduced levels of fatty alcohols did not influence the suberin polymerization process. Nevertheless, the 75% reduction in total fatty alcohol and diol loads in the seed coat resulted in increased permeability to tetrazolium salts and a higher sensitivity to abscisic acid. These results suggest that fatty alcohols and diols play an important role in determining the functional properties of the seed coat suberin barrier.Suberin is a cell wall-linked polymeric barrier that plays a critical role in the survival of plants by protecting them against various biotic and abiotic stresses. It primarily acts as a hydrophobic barrier to control the movement of water, gases, and solutes, but also contributes to the strength of the cell wall (Ranathunge et al., 2011). Suberin is deposited at the inner face of primary cell walls next to the plasma membrane (Kolattukudy, 1980; Franke and Schreiber, 2007). It is typically found as lamellae (alternating dark and light bands when viewed by transmission electron microscopy) in the endodermis, exodermis, and peridermis of roots, as well as in the peridermis of underground storage tubers (Bernards, 2002). Suberin is also found in shoot periderms of trees (i.e. cork layer) and in seed coats (Molina et al., 2006, 2008) and is deposited in response to wounding (Kolattukudy, 2001).Suberin is a polymer consisting of aliphatics (fatty acid derivatives), phenolics, and glycerol. The predominant aliphatic components of suberin are ω-hydroxy fatty acids, α,ω-dicarboxylic acids, very-long-chain fatty acids, and primary fatty alcohols, while the major phenolic components are p-hydroxycinnamic acids, especially ferulic acid (Kolatukudy, 1980; Bernards et al., 1995; Pollard et al., 2008; Ranathunge et al., 2011). In the periderm of underground storage organs, suberin is found in association with waxes, which are isolated either by extensive extraction in solvent (Soliday et al., 1979; Serra et al., 2009) or by brief immersion of tubers in chloroform (Espelie et al., 1980). These suberin-associated waxes consist of linear aliphatics (e.g. alkanes, fatty acids, and fatty alcohols), which are similar to cuticular wax components of aerial tissues but generally of shorter chain lengths (Espelie et al., 1980). In waxes extracted from 3-week-old wounded potato (Solanum tuberosum) periderms, alkyl ferulates (i.e. ferulic acid linked by an ester bond to a C16:0–C32:0 fatty alcohol) represent up to 60% of the total wax load (Schreiber et al., 2005). Root waxes are also found in 6- to 7-week-old mature taproots of Arabidopsis (Arabidopsis thaliana) with a fully developed periderm (Li et al., 2007; Kosma et al., 2012). They are enriched in alkyl hydroxycinnamates (AHCs) made of C18:0 to C22:0 fatty alcohols esterified with coumaric, caffeic, or ferulic acids (Kosma et al., 2012). The monomer composition (in terms of major chemical species and chain length) of both suberin and suberin-associated waxes varies considerably between plant species, tissues, and developmental stages. Aliphatic suberin and suberin-associated waxes are considered the major contributors to the diffusion resistance of suberized cell walls to radial transport of water and solutes (Soliday et al., 1979; Espelie et al., 1980; Zimmermann et al., 2000; Ranathunge and Schreiber, 2011). The organization of suberin components in the lamellated structure as well as how waxes may be associated with the polymer is a matter of debate (Graça and Santos, 2007).Primary fatty alcohols are long-chain hydrocarbons containing a single hydroxyl group at the terminal position. They are ubiquitously detected as components of the suberin polymer, representing 1% to 10% of the total monomer mass recovered after transesterification (Holloway, 1983; Bernards, 2002; Pollard et al., 2008). Primary fatty alcohols are also typical components of suberin-associated waxes, where they can be found either in free form or linked by an ester bond with a hydroxycinnamic acid (i.e. as AHCs; Soliday et al., 1979; Espelie et al., 1980; Bernards and Lewis 1992; Li et al., 2007; Kosma et al., 2012). In mechanically isolated endodermis of soybean (Glycine max) roots, fatty alcohols represent about 1.5% and 0.2% of the total aliphatics found in suberin-associated waxes and suberin polymer, respectively (Thomas et al., 2007). In onion (Allium cepa) root exodermis, fatty alcohols (C14:0–C28:0) account for 7% to 12% of the soluble fraction, while the suberin fraction contains only C22:0 fatty alcohol, which makes up 3% of the suberin fraction across all exodermal maturation zones (Meyer et al., 2011). In suberizing potato periderms 7 d post wounding, C16:0 to C28:0 fatty alcohols represent about 10% and 18% of the total aliphatics in the insoluble poly(aliphatic) domain (suberin polymer) and in the soluble (nonpolymeric) fraction, respectively (Yang and Bernards, 2006). A similar study on native periderms from 21-d-stored potato (Serra et al., 2009) reported that fatty alcohols represent about 20% of the total aliphatic components found in the suberin polyester, while unlinked fatty alcohols and alkyl ferulates accounted for about 23% and 44% of the total aliphatics in the soluble waxes.In Arabidopsis, C18:0, C20:0, and C22:0 fatty alcohols account for slightly less than 3% of the polymerized aliphatics in roots of soil-grown plants (Domergue et al., 2010), but as much as 36% [w/w] of the soluble wax load (Li et al., 2007). Arabidopsis fatty acyl reductases FAR1 (At5g22500), FAR4 (At3g44540), and FAR5 (At3g44550) generate, respectively, the C22:0, C20:0, and C18:0 fatty alcohol present in the suberin of root, seed coat, and wounded leaf tissues (Domergue et al., 2010). These three enzymes also generate the C18:0 to C22:0 fatty alcohol components that make up AHCs of root waxes (Kosma et al., 2012). Although one particular chain length of primary alcohol was reduced in each far single mutant line (C18:0-OH, C20:0-OH, and C22:0-OH in far5, far4, and far1, respectively), the total fatty alcohol load of the suberin polymer and its composition were only slightly affected and mutant plants had no obvious developmental or physiological defects (Domergue et al., 2010). In this study, we report on the distribution of primary fatty alcohols in the soluble (nonpolymeric) and insoluble (suberin polymer) fractions from mature roots of Arabidopsis. We report that far double and triple mutants have highly reduced fatty alcohol levels, in a chain length-specific manner, in both fractions as well as in the seed coat suberin polymer. The significant reductions in total fatty alcohol and diol levels in the seed coat of these mutants lead to increased permeability and higher sensitivity to abscisic acid (ABA), bringing to light insights on the roles of fatty alcohols and diols in determining functional properties of suberin. 相似文献
20.
The composition of suberin and lignin in endodermal cell walls (ECWs) and in rhizodermal/hypodermal cell walls (RHCWs) of
developing primary maize (Zea mays L.) roots was analysed after depolymerisation of enzymatically isolated cell wall material. Absolute suberin amounts related
to root length significantly increased from primary ECWs (Casparian strips) to secondary ECWs (suberin lamella). During further
maturation of the endodermis, reaching the final tertiary developmental state characterised by the deposition of lignified
secondary cell walls (u-shaped cell wall deposits), suberin amounts remained constant. Absolute amounts of lignin related
to root length constantly increased throughout the change from primary to tertiary ECWs. The suberin of Casparian strips contained
high amounts of carboxylic and 2-hydroxy acids, and differed substantially from the suberin of secondary and tertiary ECWs,
which was dominated by high contents of ω-hydroxycarboxylic and 1,ω-dicarboxylic acids. Furthermore, the chain-length distribution
of suberin monomers in primary ECWs ranged from C16 to C24, whereas in secondary and tertiary ECWs a shift towards higher chain lengths (C16 to C28) was observed. The lignin composition of Casparian strips (primary ECWs) showed a high syringyl content and was similar to
lignin in secondary cell walls of the tertiary ECWs, whereas lignin in secondary ECWs contained higher amounts of p-hydroxyphenyl units. The suberin and lignin compositions of RHCWs rarely changed with increasing root age. However, compared
to the suberin in ECWs, where C16 and C18 were the most prominent chain lengths, the suberin of RHCWs was dominated by the higher chain lengths (C24 and C26). The composition of RHCW lignin was similar to that of secondary-ECW lignin. Using lignin-specific antibodies, lignin epitopes
were indeed found to be located in the Casparian strip. Surprisingly, the mature suberin layers of tertiary ECWs contained
comparable amounts of lignin-like epitopes.
Received: 19 August 1998 / Accepted: 3 February 1999 相似文献