Reconstruction and forecast of doses due to ingestion of <Superscript>137</Superscript>Cs and <Superscript>90</Superscript>Sr after the Chernobyl accident

The assessment doses due to ingestion of ¹³⁷Cs and ⁹⁰Sr for the population suffering from the Chernobyl accident was performed on the basis of the new mechanistic ecological model for assessment of radiological consequences of agricultural lands contamination (EMARC). The EMARC model allows estimation of internal doses based on ecological factors influencing the contamination of foodstuff, for the post-accidental years in the countries of the former Soviet Union. The EMARC model allows estimation of all quantities required in radiation hygiene practice. For example, the proposed analytical method may be used for both retrospective dose reconstruction and prospective estimates of annual dose and integrated “life-time” dose, for different age intervals. According to the EMARC model, estimated reference “life-time” doses for adults are between 7 and 269 μSv kBq⁻¹ m² for ¹³⁷Cs, and between 25 and 235 μSv kBq⁻¹ m² for ⁹⁰Sr. Maximal doses were estimated for persons who were 3, 9 and 11 years old, at the time of the accident and these doses exceed those for adults by a factors of 1, 5 for ⁹⁰Sr, and 1.4 for ¹³⁷Cs.     

<Superscript>137</Superscript>Cs, <Superscript>40</Superscript>K, <Superscript>238</Superscript>Pu, <Superscript>239+240</Superscript>Pu and <Superscript>90</Superscript>Sr in biological samples from King George Island (Southern Shetlands) in Antarctica

There are few data reported on radionuclide contamination in Antarctica. The aim of this paper is to report ¹³⁷Cs, ⁹⁰Sr and ^238,239+240Pu and ⁴⁰K activity concentrations measured in biological samples collected from King George Island (Southern Shetlands, Antarctica), mostly during 2001–2002. The samples included: bones, eggshells and feathers of penguin Pygoscelis papua, bones and feathers of petrel Daption capense, bones and fur of seal Mirounga leonina, algae Himantothallus grandifolius, Desmarestia anceps and Cystosphaera jacquinotii, fish Notothenia corriceps, sea invertebrates Amphipoda, shells of limpet Nacella concina, lichen Usnea aurantiaco-atra, vascular plants Deschampsia antarctica and Colobanthus quitensis, fungi Omphalina pyxidata, moss Sanionia uncinata and soil. The results show a large variation in some activity concentrations. Samples from the marine environment had lower contamination levels than those from terrestrial ecosystems. The highest activity concentrations for all radionuclides were found in lichen and, to a lesser extent, in mosses, probably because lichens take up atmospheric pollutants and retain them. The only significant correlation (except for that expected between ²³⁸Pu and ^239+240Pu) was noted for moss and lichen samples between plutonium and ⁹⁰Sr. A tendency to a slow decrease with time seems to be occurring. Analyses of the activity ratios show varying fractionation between various radionuclides in different organisms. Algae were relatively more highly contaminated with plutonium and radiostrontium, and depleted with radiocesium. Feathers had the lowest plutonium concentrations. Radiostrontium and, to a lesser extent, Pu accumulated in bones. The present low intensity of fallout in Antarctic has a lower ²³⁸Pu/^239+240Pu activity ratio than that expected for global fallout.     

Doses from beta radiation in sensitive layers of human lung and dose conversion factors due to <Superscript>222</Superscript>Rn/<Superscript>220</Superscript>Rn progeny

Great deal of work has been devoted to determine doses from alpha particles emitted by ²²²Rn and ²²⁰Rn progeny. In contrast, contribution of beta particles to total dose has been neglected by most of the authors. The present work describes a study of the detriment of ²²²Rn and ²²⁰Rn progeny to the human lung due to beta particles. The dose conversion factor (DCF) was introduced to relate effective dose and exposure to radon progeny; it is defined as effective dose per unit exposure to inhaled radon or thoron progeny. Doses and DCFs were determined for beta radiation in sensitive layers of bronchi (BB) and bronchioles (bb), taking into account inhaled ²²²Rn and ²²⁰Rn progeny deposited in mucus and cilia layer. The nuclei columnar secretory and short basal cells were considered to be sensitive target layers. For dose calculation, electron-absorbed fractions (AFs) in the sensitive layers of the BB and bb regions were used. Activities in the fast and slow mucus of the BB and bb regions were obtained using the LUNGDOSE software developed earlier. Calculated DCFs due to beta radiation were 0.21 mSv/WLM for ²²²Rn and 0.06 mSv/WLM for ²²⁰Rn progeny. In addition, the influence of Jacobi room parameters on DCFs was investigated, and it was shown that DCFs vary with these parameters by up to 50%.     

Activity concentrations of <Superscript>226</Superscript>Ra, <Superscript>228</Superscript>Th,and <Superscript>40</Superscript>K in different food crops from a high background radiation area in Bitsichi,Jos Plateau,Nigeria

One of the three goals of the United Nations for sustainable food security is to ensure that all people have access to sufficient, nutritionally adequate, and safe food. Decades of tin mining in the Bitsichi area of the Jos Plateau, Nigeria, have left a legacy of polluted water supplies, impoverished agricultural land, and soil containing abnormally high levels of naturally occurring radioactive elements. In order to ascertain the radiological food safety of the population, different crops that constitute the major food nutritive requirements were collected directly across farmlands in the area. The activity concentrations of ²²⁶Ra, ²²⁸Th, and ⁴⁰K were determined in the food and soil samples using γ-ray spectrometry. Additionally, in situ gamma dose rate measurements were performed on the farms using a pre-calibrated survey meter. The corresponding activity concentrations in the food crops ranged from below detection limit (BDL) to 684.5 Bq kg⁻¹ for ⁴⁰K, from BDL to 83.5 Bq kg⁻¹ for ²²⁶Ra, and from BDL to 89.8 Bq kg⁻¹ for ²²⁸Th. Activity concentrations of these radionuclides were found to be lower in cereals than in tubers and vegetables. As for the soil samples, activity concentrations of these radionuclides varied from BDL to 166.4 Bq kg⁻¹, from 10.9 to 470.6 Bq kg⁻¹, and from 122.7 to 2,189.5 Bq kg⁻¹ for ⁴⁰K, ²²⁶Ra, and ²²⁸Th, respectively. Average external gamma dose rates were found to vary across the farms from 0.50 ± 0.01 to 1.47 ± 0.04 μSv h⁻¹. Due to past mining activities, the soil radioactivity in the area has been modified and the concentration level of the investigated natural radionuclides in the food crops has also been enhanced. However, the values obtained suggest that the dose from intake of these radionuclides by the food crops is low and that harmful health effects are not expected.     

Soil-to-grain transfer of fallout <Superscript>137</Superscript>Cs for 28 winter wheat cultivars as observed in field experiments

In order to find wheat cultivars with a minimum soil-to-grain transfer of fallout (137)Cs, 28 winter wheat cultivars were investigated at 3 different sites with different soil types in Bavaria, Germany. Each cultivar was grown on an area of 10 m(2) and harvested in August 1999. The soil-to-grain concentration ratios (C(r)) of (137)Cs varied by a factor of up to 3 from cultivar to cultivar at a given site and from site-to-site for a given cultivar. The mean C(r) values at the three sites, 4.2 x 10(-4), 4.9 x 10(-4) and 7.5 x 10(-4), differed significantly. The fact that no cultivar showed similar C(r) values at the three sites indicates a strong influence of the soil on C(r). The cultivars Flair, Kornett and Previa showed a minor uptake of (137)Cs compared with the mean of all cultivars at each site. Unlike (137)Cs, the (40)K concentrations in the wheat grains varied only within a small range (122-190 Bq kg(-1)) at each site, which is due to the potassium regulation by the plants. For both radionuclides, the differences between the root uptake characteristics of the cultivars may not only be explained by an inter-cultivar variability due to genetic differences between the cultivars, but also by an intra-cultivar variability due to different soil conditions.     

A long-term study of catchment inputs of <Superscript>137</Superscript>Cs to a subalpine lake in the form of allochthonous terrestrial plant material

The input of allochthonous plant material, largely terrestrial in origin, during the spring spate is a major source of primary production for montane lake ecosystems such as Øvre Heimdalsvatn and is readily incorporated into the food chain leading to fish. The transport from the lake catchment of allochthonous plant material contaminated with Chernobyl ¹³⁷Cs in 1986 was investigated from 1989 to 2008. ¹³⁷Cs activity concentrations were determined for both total samples before sorting and for the separate components in the two main tributary streams, Brurskardbekken and Lektorbekken, as well as the outflow river, Hinøgla. The total samples showed a similar long-term decline at all sites, although ¹³⁷Cs activity concentrations were generally somewhat higher in Hinøgla compared to the tributary streams. For the total samples, ecological half-lives were in the region of 4–5 years. However, there were major differences between components. The ¹³⁷Cs activity content of most components, including mosses, leaves of willow and woody material, decreased significantly over time. Lichens and juniper declined, but the relationship was not significant, while leaves of mountain birch and dwarf birch showed no obvious trend over the 20-year period.     

Dosimetric comparison between realistic ocular model and other models for COMS plaque brachytherapy with <Superscript>103</Superscript>Pd, <Superscript>131</Superscript>Cs,and <Superscript>125</Superscript>I radioisotopes

Nowadays, Monte Carlo calculations are commonly used for the evaluation of dose distributions and dose volume histograms in eye brachytherapy. However, currently available eye models have simple geometries, and main substructures of the eye are either not defined in details or not distinguished at all. In this work absorbed doses of eye substructures have been estimated for eye plaque brachytherapy using the most realistic eye model available, and compared with absorbed doses obtained with other available eye models. For this, a medium-sized tumour on the left sides of the right eye was considered. Dosimetry calculations were performed for four different eye models developed based on a literature review, and using a 12 mm Collaborative Ocular Melanoma Study plaque containing ¹³¹Cs, ¹⁰³Pd, and ¹²⁵I sources. Obtained results illustrate that the estimated doses received by different eye substructures strongly depend on the model used to represent the eye. It is shown here that using a non-realistic eye model leads to a wrong estimation of doses for some eye substructures. For example, dose differences of up to 35% were observed between the models proposed by Nogueira and co-workers and Yoriyaz and co-workers, while doses obtained by use of the models proposed by Lesperance and co-workers, and Behrens and co-workers differed up to 100 and 63% as compared to the situation when a realistic model was used, respectively. Moreover, comparing different radionuclides showed that the most uniform dose distribution in the considered tumour region was that from ¹³¹Cs, with a coefficient of variation of 33%. In addition, considering the realistic eye model, it was found that the radiosensitive region of the lens received more than the threshold dose of cataract induction (0.5 Gy), for all investigated radionuclides.     

Age-dependent accumulation of <Superscript>137</Superscript>Cs by pike <Emphasis Type="Italic">Esox lucius</Emphasis> in the Yenisei River

Age-dependent accumulation of ¹³⁷Cs in the muscles and bodies of the pike Esox lucius (aged two to seven years) inhabiting a section of the Yenisei River polluted with artificial radionuclides has been studied. The content of ¹³⁷Cs in muscles varied from 0.5 to 7.0 Bq/kg of fresh weight. The maximum content of the radionuclide has been found in juveniles. The content of ¹³⁷Cs in pike muscles and body decreased considerably with age. The high content of ¹³⁷Cs in the muscles of juveniles is probably a consequence of their higher intensity of feeding as compared to older individuals, which is due to the intense growth of juveniles.     

Behavior of <Superscript>137</Superscript>Cs in the soil-rhizosphere-plant system (by the example of the Yenisei River floodplain)

¹³⁷Cs speciation and distribution in the components of the biogeocenosis of the Yenisei River floodplain such as soils, rhizosphere, and plants were studied. The study was carried out near zones of influence by the Krasnoyarsk Mining and Chemical Combine (MCC). Variation in the specific activity of radionuclides with increasing distance from the pollution source and its ratio in the soil and rhizosphere and distribution in the plant body were studied. A variety of occurrence forms of ¹³⁷Cs, including mobile ones, was revealed. The amount of ¹³⁷Cs leached from plants during flooding was calculated from the results of laboratory experiments.     

The dynamic transfer of <Superscript>3</Superscript>H and <Superscript>14</Superscript>C in mammals: a proposed generic model

Associated with the present debate regarding the potential revival of nuclear energy there is an increased interest in assessing the radiological risk to the public and also the environment. Tritium and ¹⁴C are key radionuclides of interest in many circumstances (e.g. heavy water reactors, waste storage and fusion reactors). Because the stable analogues of these two radionuclides are integral to most biological compounds, their modelling should follow general principles from life sciences. In this paper, a model of the dynamics of ¹⁴C and ³H in mammals is proposed on the basis of metabolic understanding and of, as far as possible, readily available data (e.g. for organ composition and metabolism). The model is described together with validation tests (without calibration) for a range of farm animals. Despite simplifications, the model tests are encouraging for a range of animal types and products (tissues and milk), and further improvements are suggested.     

First principle calculations of <Superscript>113</Superscript>Cd chemical shifts for proteins and model systems

¹¹³Cd isotropic NMR shieldings are calculated for a number of metal ion binding sites in proteins, using the GIAO-B3LYP and GIAO-HF methods with the uncontracted (19s15p9d4f) polarized basis set of Kellö and Sadlej on cadmium and 6-31G(d) on the ligands. The results compare favorably with experimental data, indicating that first principle calculations are a useful tool for structural interpretation of ¹¹³Cd chemical shift data from metal ion containing proteins. The effect of different ligand types (thiolate, imidazole, water, and monodentate carboxylate), coordination number, and deviations of the coordination geometry from ideal structures is evaluated. In particular, the ligand type and coordination number are important factors, but also changes in cadmium–ligand bond lengths may cause significant changes of the chemical shift.     

Influence of human biokinetics of strontium on internal ingestion dose of <Superscript>90</Superscript>Sr and absorbed dose of <Superscript>89</Superscript>Sr to organs and metastases

The objective of the present work is to apply the plasma clearance parameters to strontium, previously determined in our laboratory, to improve the biokinetic and dosimetric models of strontium-90 (⁹⁰Sr) used in radiological protection; and also to apply this data for the estimation of the radiation doses from strontium-89 (⁸⁹Sr) after administration to patients for the treatment of the painful bone metastases. Plasma clearance and urinary excretion of stable strontium tracers of strontium-84 (⁸⁴Sr) and strontium-86 (⁸⁶Sr) were measured in GSF-National Research Center for Environment and Health (GSF) in 13 healthy German adult subjects after intravenous injection and oral administration. The biological half-life of strontium in plasma was evaluated from 49 plasma concentration data sets following intravenous injections. This value was used to determine the transfer rates from plasma to other organs and tissues. At the same time, the long-term retention of strontium in soft tissue and whole body was constrained to be consistent with measured values available. A physiological urinary path was integrated into the biokinetic model of strontium. Parameters were estimated using our own measured urinary excretion values. Retention and excretion of strontium were modeled using compartmental transfer rates published by the International Commission on Radiological Protection (ICRP), the SENES Oak Ridge Inc. (SENES), and the Urals Research Center for Radiation Medicine (TBM). The results were compared with values calculated by applying our GSF parameters (GSF). For the dose estimation of ⁸⁹Sr, a bone metastases model (GSF-M) was developed by adding a compartment, representing the metastases, into the strontium biokinetic model. The related parameters were evaluated based on measured data available in the literature. A set of biokinetic parameters was optimized to represent not only the early plasma kinetics of strontium but also the long-term retention measured in soft tissue and whole body. The ingestion dose coefficients of ⁹⁰Sr were computed and compared with different biokinetic model parameters. The ingestion dose coefficients were calculated as 2.8 × 10⁻⁸, 2.1 × 10⁻⁸, 2.5 × 10⁻⁸ and 3.8 × 10⁻⁸ Sv Bq⁻¹ for ICRP, SENES, TBM and GSF model parameters, respectively. Moreover, organ absorbed dose for the radiopharmaceutical of ⁸⁹Sr in bone metastases therapy was estimated based on the GSF and ICRP biokinetic model parameters. The effective doses were 3.3, 1.8 and 1.2 mSv MBq⁻¹ by GSF, GSF-M, and ICRP Publication 67 model parameters, respectively, compared to the value of 3.1 mSv MBq⁻¹ reported by ICRP Publication 80. The absorbed doses of red bone marrow and bone surface, 17 and 21 mGy MBq⁻¹ calculated by GSF parameters, and 7.1 and 8.8 mGy MBq⁻¹ by GSF-M parameters, are comparable to the clinical results of 3–19 mGy MBq⁻¹ for bone marrow and 16 mGy MBq⁻¹ for bone surface. Based on the GSF-M model, the absorbed dose of ⁸⁹Sr to metastases was estimated to be 434 mGy MBq⁻¹. The strontium clearance half-life of 0.25 h from the plasma obtained in the present study is obviously faster than the value of 1.1 h recommended by ICRP. There are no significant changes for ingestion dose coefficients of ⁹⁰Sr using different model parameters. A model including the metastases was particularly developed for dose estimation of ⁸⁹Sr treatment for the pain of bone metastases.     

External Ni<Superscript>2+</Superscript> and ENaC in A6 Cells: Na<Superscript>+</Superscript> Current Stimulation by Competition at a Binding Site for Amiloride and Na<Superscript>+</Superscript>

In cultured A6 monolayers from distal Xenopus kidney, external Ni²⁺ stimulated active Na⁺ uptake via the epithelial Na⁺ channel, ENaC. Transepithelial capacitance measurements ruled out exocytosis of ENaC-containing vesicles underlying the Ni²⁺ effect. Na⁺ current noise analysis was performed using the neutral Na⁺-channel blocker 6-chloro-3,5-diamino-pyrazine-2-carboxamide (CDPC) and amiloride. The analysis of CDPC-induced noise in terms of a three-state channel model revealed that Ni²⁺ elicits an increase in the number of open channels as well as in the spontaneous open probability. While Ni²⁺ had no influence on CDPC-blocker kinetics, the macroscopic and microscopic blocking kinetics of amiloride were affected. Ni²⁺ turned out to compete with amiloride for a putative binding site but not with CDPC. Moreover, external Na⁺—known to compete with amiloride and so producing the self-inhibition phenomenon—and Ni²⁺ exerted mutually exclusive analogous effects on amiloride kinetics. Na⁺ current kinetics revealed that Ni²⁺ prevents ENaC to be downregulated by self-inhibition. Co²⁺ behaved similarly to Ni²⁺, whereas Zn²⁺ did not. Attempts to disclose the chemical nature of the site reacting with Ni²⁺ suggested cysteine but not histidine as reaction partner.     

<Superscript>131</Superscript>I age-dependent inhalation dose in Southern Poland from Fukushima accident

A general method for calculating doses absorbed from isotopes released in nuclear accidents is presented. As an example, this method was used to calculate doses for inhabitants of Southern Poland due to inhalation of ¹³¹I released due to the Fukushima nuclear plant accident. ¹³¹I activity measurements in the air of that region provided the basis for the study. The proposed model is based on a complex biokinetic model for iodine merging the Leggett model developed in 2010 with the human respiratory tract and gastrointestinal tract models recommended by the International Commission on Radiological Protection (ICRP). This model is described here, and it is demonstrated that resulting dose estimates are consistent with those obtained using the ICRP methodology. Using the developed model, total doses were calculated for six age groups of both genders, for gaseous and aerosol fractions alike. The committed effective dose, H ₅₀, for an adult man reached 16 nSv, which is lower than 0.001% of the background dose. The dose for the thyroid of an adult reached 0.33 μSv, which corresponds to circa 0.0007% of the dose to the population of Southern Poland after the Chernobyl nuclear plant accident.     

Vacuolar Na<Superscript>+</Superscript>/H<Superscript>+</Superscript> antiporter from barley: Identification and response to salt stress

One of the protective mechanisms used by plants to survive under conditions of salt stress caused by high NaCl concentration is the removal of Na+ from the cytoplasm. This mechanism involves a number of Na+/H+-antiporter proteins that are localized in plant plasma and vacuolar membranes. Due to the driving force of the electrochemical H+ gradient created by membrane H+-pumps (H+-ATPases and vacuolar H+-pyrophosphatases), Na+/H+-antiporters extrude sodium ions from the cytoplasm in exchange for protons. In this study, we have identified the gene for the barley vacuolar Na+/H+-antiporter HvNHX2 using the RACE (rapid amplification of cDNA ends)-PCR (polymerase chain reaction) technique. It is shown that the identified gene is expressed in roots, stems, and leaves of barley seedlings and that it presumably encodes a 59.6 kD protein composed of 546 amino acid residues. Antibodies against the C-terminal fragment of HvNHX2 were generated. It is shown that the quantity of HvNHX2 in tonoplast vesicles isolated from roots of barley seedlings remains the same, whereas the rate of Na+/H+ exchange across these membranes increases in response to salt stress. The 14-3-3-binding motif Lys-Lys-Glu-Ser-His-Pro (371-376) was detected in the HvNHX2 amino acid sequence, which is suggestive of possible involvement of the 14-3-3 proteins in the regulation of HvNHX2 function.     

Measurement of activity concentrations of <Superscript>40</Superscript>K, <Superscript>226</Superscript>Ra and <Superscript>232</Superscript>Th for assessment of radiation hazards from soils of the southwestern region of Nigeria

Activity concentrations of the selected radionuclides ⁴⁰K, ²²⁶Ra and ²³²Th were measured in surface soil samples collected from 38 cities in the southwest region of Nigeria by means of gamma spectroscopy with a high-purity germanium detector. Measured activity concentration values of ⁴⁰K varied from 34.9 ± 4.4 to 1,358.6 ± 28.5 Bq kg⁻¹ (given on a dry mass (DM) basis) with a mean value of 286.5 ± 308.5 Bq kg⁻¹; that of ²²⁶Ra varied from 9.3 ± 3.7 to 198.1 ± 13.8 Bq kg⁻¹ with a mean value of 54.5 Bq kg⁻¹ and a standard deviation of 38.7 Bq kg⁻¹, while that of ²³²Th varied from 5.4 ± 1.1 to 502.0 ± 16.5 Bq kg⁻¹ with a mean value of 91.1 Bq kg⁻¹ and standard deviation of 100.9 Bq kg⁻¹. The mean activity concentration values obtained for ²²⁶Ra and ²³²Th are greater than the world average values reported by the United Nations Scientific Committee on Effects of Atomic Radiation for areas of normal background radiation. Radiological indices were estimated for the radiation/health hazards of the natural radioactivity of all soil samples. Estimated absorbed dose rates in air varied from 12.42 ± 2.25 to 451.33 ± 19.06 nGy h⁻¹, annual outdoor effective dose rates from 0.015 ± 0.003 to 0.554 ± 0.023 mSv year⁻¹, internal hazard index from 0.10 ± 0.03 to 3.02 ± 0.16, external hazard index from 0.07 ± 0.01 to 2.60 ± 0.11, representative level index from 0.19 ± 0.03 to 6.84 ± 0.29, activity index from 0.09 ± 0.02 to 3.42 ± 0.15, and radium equivalent activity from 26.95 ± 5.04 to 963.15 ± 41.87 Bq kg⁻¹. Only the mean value of the representative level index exceeds the limit for areas of normal background radiation. All other indices show mean values that are lower than the recommended limits.     

<Superscript>1</Superscript>H, <Superscript>13</Superscript>C and <Superscript>15</Superscript>N NMR assignments of a calcium-binding protein from <Emphasis Type="Italic">Entamoeba histolytica</Emphasis>

We report almost complete sequence specific ¹H, ¹³C and ¹⁵N NMR assignments of a 150-residue long calmodulin-like calcium-binding protein from Entamoeba histolytica (EhCaBP6), as a prelude to its structural and functional characterization.