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1.
Summary The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using -glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and Kao (1978). Weak and infrequent reactions are obtained in multivesicular bodies (mvbs) and multilamellar bodies (mlbs) derived from them. Residual bodies (rbs) begin to react strongly as they lyse. Source of AcPhs is endoplasmic reticulum which has barely differentiated towards the GERL configuration; it becomes reactive as it is incorporated into secondary lysosomes. GERL tubules, Y-bodies and vesicles respond erratically and weakly, and are also incorporated into rbs. No evidence was found for a significant participation of Golgi bodies in these processes, and acid phosphatase cytochemistry fails to reveal a topographical relationship between GERL in these cells and Golgi saccules. Coated vesicle clusters found in the predawn receptive segments are AcPh-negative; this implies that their previous identification as GERL-derived Nebenkerne carrying hydrolytic enzymes to newly-formed mvbs (Blest, Kao and Powell, 1978) is dubious. Isolation bodies and autophagic vacuoles enclosing other organelles in pathological receptors give strong reactions while adjacent secondary lysosomes derived from rhabdomere membrane and associated GERL give weak ones. It is concluded that rhabdomere-derived rb lysis is more tightly regulated than other autophagic processes, and it is suggested that a high degree of control is necessary in a receptor which may repeat the autophagy of a large mass of transductive membrane at least 60–100 times in the course of its working life.The authors thank Professor D.T. Anderson F.R.S. for the use of field facilities at the Crommelin Biological Field Station of Sydney University at Warrah, Pearl Beach, New South Wales throughout all these studies; Dr. Gary Griffiths (EMBO, Heidelberg) and Dr. Alex Pyliotis (Biochemistry, SGS, Australian National University) for some helpful comments on acid phosphatase histochemistry; Sally J. Stowe for help in the field; and Rod Whitty and the staff on the Electron Microscopy Unit for advice and support. Figure 28 was prepared by Chris Snoek 相似文献
2.
Summary In the spider Dinopis, retinae of the posterior median eyes synthesise new photoreceptor membrane in bulk at dusk and destroy it at dawn (Blest, 1978). During the dawn period, there is a rapid, anticipatory differentiation of unusual organelles from the rough endoplasmic reticulum (RER) in the intermediate segments of the receptors. This system is classified as GERL. Its products appear to play a role in the autolysis of photoreceptor membrane. Consistent topographical relationship to Golgi bodies has not been determined. Circumscribed regions of RER whorls first reorganise to yield fenestrated profiles; these differentiate further to a number of structures by condensation and loss of ribosomes. Smooth tubular profiles are termed rigid tubules to indicate their probable homology with the rigid lamellae of vertebrate secretory cells. More complex smooth multilocular bodies are also produced. Evidence is discussed which implies that both rigid tubules and multilocular bodies give rise to condensing vacuoles. These, in turn, pinch off coated vesicles assembled as Nebenkerne. Some rigid tubules are transported to the interrhabdomeral cytoplasm of the receptive segments. At late stages of differentiation, rigid tubules, multilocular bodies and Nebenkerne give strong, positive responses to zinc iodide-osmium tetroxide (ZIO) treatment; early stages and both cis and trans Golgi components do not. GERL differentiation is independent of immediate illumination of the retina at dawn. It is suggested to mediate the lysis of membrane degradation products by the production of hydrolases.We thank Professor D.T. Anderson F.R.S. for our use of field facilities at the Crommelin Biological Field Station of Sydney University at Warrah, Pearl Beach, N.S.W., and Andrew and Sally Austin and Sally Stowe for help in the field. We are indebted to Rod Whitty and the Electron Microscopy Unit for advice and support throughout these studies 相似文献
3.
Summary Dawn photoreceptor breakdown in the crab Leptograpsus variegatus is analysed at the ultrastructural level. Coated vesicles derived from microvilli are assembled as multivesicular bodies (mvbs), which degrade to multilamellar bodies (mls) and are lysed. Cytochemical markers for hydrolases were a fluorideinhibited -glycerophosphatase and a fluoride-insensitive p-nitrophenyl phosphatase, with indistinguishable distributions when localised at pH 5.0. These enzymes are injected into the secondary lysosomes from two sources: (i) Immediately after dawn Golgi bodies are highly active, and differentiate a transtubular network, from which tubules and vesicles detach, and can be seen fusing with mvbs and mlbs. (ii) Saccules derived from the rough endoplasmic reticulum (RER) provide a second source and are most often seen in association with late mlbs. Both kinds of primary lysosome rarely give AcPh-positive responses when free in the cytosol, but are seen to do so as they make contact with their secondary lysosomal targets. Lipid droplets and lipofuscin bodies are interpreted as the residual products of breakdown. These results are discussed in relation to previous findings on photoreceptor membrane breakdown in a dinopid spider. Attention is drawn to the implied diversity of organisation of lysosomal compartments in receptors which internalise membranes of similar compositions.The authors thank Professor T.H. Waterman for hospitality extended to A.D. Blest at Yale University, for allowing them to see results prior to publication, and for enthusiastic encouragement of at present project during a visit to this University in 1979. We also thank Dr. Gary Hafner and Dr. D.R. Nassel for sending us results prior to publication, and others, especially Dr. Dean Bok, for discussions in correspondence. Bruce Ham helped to collect crabs. Rod Whitty and the staff of the Electron Microscope Unit provided advice and support throughout these studies. Chris Snoek prepared Fig. 1 a, b 相似文献
4.
Summary Intermediate segment outgrowths (ISOs) are transitory specialisations of the plasma membrane of intermediate segments of the posterior median photoreceptors of Dinopis. Local regions form outgrowths into the glial partitions separating the receptors and remain connected to their parent intermediate segments by narrow necks. ISOs, only a few m in diameter, are sites of intense endocytosis. Coated pits in their plasma membranes give rise to saccular internalisations. Unusual, slender, endocytotic tubules either pinch off coated vesicles, or become detached to yield a tubular detritus. Products of endocytosis are assembled to yield multivesicular and dense bodies, which are usually surrounded by smooth saccules derived from the endoplasmic reticulum of the intermediate segment. ISOs also contain arrays of tubules, thought to be stacks of haemocyanin molecules. There are usually at least 10 times the number of empty ISOs as full and active outgrowths. The number of active ISOs increases rapidly at dawn to peak at about 3 h after sunrise and then rapidly declines. The present sample suggests that the number of empty ISOs increases steadily throughout the day. Thus, ISOs turn over and are probably rapidly formed and short-lived. The contents of ISOs are observed to be evicted into the intermediate segments, where they presumably join the population of secondary lysosomes and are digested. The cyclical activity of ISOs is greatest after shedding of rhabdomeral membrane at dawn (Blest 1978). ISOs are thought to be concerned with the return to the receptors of a minor fraction of rhabdomeral material lost to extracellular space during shedding of the microvillar membrane.The authors thank Professor D.T. Anderson, F.R.S., for use of field facilities of the Crommelin Biological Field Station of Sydney University at Warrah, Pearl Beach, N.S.W., and Andrew and Sally Austin and Carolyn Lawson-Kerr for help in the field. We are indebted to Rod Whitty and the Electron Microscopy Unit for support during these studies, and to Gary Brown for preparing Figs. 1 and 16 相似文献
5.
Summary In order to study the synthesis and degradation processes of the photoreceptor membranes in the abalone, Nordotis discus, the localization of acyltransferase and acid hydrolase activities, respectively, were determined at the electron-microscopic level. Acyltransferase activity was localized on the cytoplasmic sides of thick (>10 nm) membranes of the following organelles: a few cisternae at the trans (or concave) side of Golgi apparatus, Golgi and probably related vesicles, short tubules, curved pentalaminar disks and limiting membranes of the phagosomal multivesicular bodies; all organelles were scattered in the peri- to supranuclear cytoplasm. The phospholipids, which are major components of the photoreceptor membrane, are considered to be synthesized by these membranes. Acid phosphatase activity was localized in the lumina of Golgi cisternae and vesicles, lysosomes, and smaller multivesicular and related bodies, but not in multilamellar bodies. The matrices of the larger multivesicular bodies and of the pigment granule complexes showed arylsulfatase activity. Vesiculated and autophagocytosed photoreceptor microvilli seemed to be degraded by acid hydrolases, forming multivesicular and related bodies. Supporting cells also showed acyltransferase and acid hydrolase activities.Abbreviations used in this Paper AcP
acid phosphatase
- ArS
arylsulfatase
- AT
acyltransferase
- ER
endoplasmic reticulum
- GERL
Golgi-endoplasmic reticulum-lysosomal complex
- MEB
meshwork body
- MLB
multilamellar body
- MVB
multivesicular body
- VLB
vesiculolamellar body 相似文献
6.
Summary The fine structure of the secretory tubules in the kidney of the aglomerular goose-fish (Lophius piscatorius) is described. The cells have a pyramidal shape, are joined together by multiple desmosomes, and share as main characteristics: abundant and deep inflections of the basal and lateral cell membranes; coated luminal plasma membranes forming multiple microvilli or a genuine brush border; moderate numbers of comparatively small mitochondria, usually unassociated with the basal and lateral plasma membrane specializations; numerous multivesicular bodies occuring in the apical cytoplasm; abundant large lysosome-like bodies in the intermediate regions of the cytoplasm; and comparatively poor development of endoplasmic reticulum and Golgi apparatus.The observations suggest that the cells perform both absorptive and secretory functions and are metabolically unusually active in autolytic and heterolytic work. Comparisons with other aglomerular species indicate that the ability for active secretory function is not necessarily dependent on a close association between plasma membrane and mitochondria; however, this ability does appear to require a markedly increased basal and/or lateral cell surface created by multiple invaginations of the plasma membrane. The abundance of desmosomes and associated structures appears to represent a unique structural specialization of the goosefish tubule, and indicates that the cells must be firmly anchored to one another to supply a rigid and mechanically continuous lining of the tubule. The multivesicular bodies probably represent endocytic vacuoles which fuse with apical vesicles and invaginate their outer membrane to form the internal vesicles; they appear to transform to ambilysosomes via a function as heterophagosomes and — later — combined hetero- and autophagosomes.Supported by grants from Karolinska Institutet, Fonden til Videnskabens Fremme and Konsul Johannes Fogh-Nielsen og fru Ella Fogh-Nielsens Légat. Part of the study was performed at the Zoological Station at Naples, Italy. The assistance of Mrs. Britt-Marie Karlsson is gratefully acknowledged. 相似文献
7.
The formation of protein-carbohydrate yolk in the statoblast of a fresh-water bryozoan, Pectinatella gelatinosa, was studied by electron microscopy. Two types (I and II) of yolk cells were distinguished. The type I yolk cells are mononucleate and comprise a large majority of the yolk cells. The type II yolk cells are small in number; they become multinucleate by fusion of cells at an early stage of vitellogenesis. In both types of yolk cells, electron-dense granules (dense bodies) are formed in Golgi or condensing vacuoles, which are then called yolk granules. For the formation of yolk granules, the following processes are considered: 1. Yolk protein is synthesized in the rough-surfaced endoplasmic reticulum (RER) of the yolk cells. 2. The synthesized protein condenses in the cisternal space of the RER and is packaged into small oval swellings, which are then released from the RER as small vesicles (Golgi vesicles, 300-600 A in diameter). 3. The small vesicles fuse with one another to form condensing vacuoles, or with pre-existing growing yolk granules. 4. In the matrix of the condensing vacuoles or growing yolk granules, electron-dense fibers are fabricated and then arranged in a paracrystalline pattern to form the dense body. 5. After the dense body reaches its full size, excess membrane is removed and eventually the yolk granules come to mature. Toward the end of vitellogenesis of the yolk cells, the cytoplasmic organelles are ingested by autophagosomes derived from multivesicular bodies and disappear. 相似文献
8.
In addition to demonstrating synaptic vesicles, staining with the zinc-iodide-osmium tetroxide (ZIO) method reveals the presence of positively reacting GERL membranes in association with the Golgi complex and lysosomes in the nerve cell bodies within ganglia from the locust Schistocerca gregaria and the gastropod molluscs, Limnaea stagnalis and Helix aspersa. A positive response to ZIO occurs in certain Golgi vesicles and saccules, in GERL (Golgi-endoplasmic-reticulum-lysosomes), in multivesicular bodies as well as residual bodies and in small vesicles and cisternae of axonal smooth endoplasmic reticllum (ER). The interrelationships between these organelles are considered in view of the similarity of the ZIO localization to phosphatase-rich sites in the neuronal perikarya and with respect to the possibility that components of the synaptic vesicles are formed in the Golgi region of the cell and migrate via the axonal smooth ER to the synaptic regions. 相似文献
9.
Summary The ultrastructure of freeze-substituted (FS) hyphae ofLaetisaria arvalis is described and compared to that of similar hyphae preserved by conventional chemical fixation (CF). The outline of membrane-bound organelles as well as the plasma membrane was smooth in FS cells. In contrast, hyphae preserved by CF exhibited membrane profiles that were extremely irregular. Centers of presumed Golgi activity were best preserved by FS. Microvesicles, 27–45 nm diameter and hexagonal in transverse section, were observed most readily in FS cells. Filasomes (= microvesicles within a filamentous matrix) were only observed in FS cells. Apical vesicles, 70–120 nm diameter, associated with the centers of Golgi activity and within the Spitzenkörper region exhibited finely granular matrices in FS hyphae, whereas in CF hyphae the contents were coarsely fibrous and less electron-dense. Microvesicles were present at hyphal apices and regions of septa formation. Filasomes were also found at regions of septa formation as well as along lateral hyphal tip cell walls. Microvesicles, but not filasomes, were observed in membrane-bound vesicles (= multivesicular bodies) and in larger vacuoles. Filaments, 5.2–5.4 nm wide, were juxtaposed with centripetally developing septa. Cytoplasmic inclusions, 20–40 m in length, composed of bundles of 6.7–8.0 nm wide filaments were observed in both FS and CF hyphae. 相似文献
10.
多泡体形成过程的细胞化学研究 总被引:1,自引:0,他引:1
Multivesicular bodies were observed frequently in electron microscope photographs of Leydig cells from normal adult rat testes. Their formation, evolution and fate were analyzed morphologically in preparations treated to show cytidine monophosphatase (CMPase) activity and in animals sacrificed at various time intervals ranging from 5 min to 2 hrs after a single intratesticular injection of cationic ferritin (CF). Analysis of morphological and cytochemical data led to the following interpretation for the origin and fate of the multivesicular bodies in Leydig cells. The formation of multivesicular bodies in Leydig cells can be divided into three steps. Step 1, some endocytic vacuoles in Golgi region fuse with small vesicles to form pre-multivesicular bodies. Step 2, the pre-multivesicular bodies fuse together to form pale multivesicular bodies which are characterized by their large size, pale matrix and paucity of internal vesicles. Step 3, the pale multivesicular bodies remove their surplus enveloping membrane to become dense multivesicular bodies which are characterized by their smaller size, dense matrix and filling with internal vesicles. The pre-multivesicular bodies and pale multivesicular bodies do not contain hydrolytic enzymes, the dense multivesicular bodies acquire their hydrolytic enzymes by fusion with lysosomes and show CMPase activity. The dense multivesicular bodies often show a very close association with autophagosomes, and they might be involved in the autophagic activity of Leydig cells. 相似文献
11.
本实验用酶细胞化学和示踪细胞化学方法观察了睾丸间质细胞中多泡体的形成过程及其与溶酶体的关系。实验结果表明,睾丸间质细胞中多泡体的形成可分三个阶段:首先,一些含内吞物质的泡状结构进入高尔基体区域,与那里的小泡融合,形成内含少量小泡的前多泡体;然后,前多泡体互相融合,形成体积较大、基质电子密度低、内含小泡排列稀疏的低电子密度多泡体;最后,低电子密度多泡体通过表面长出微绒毛样结构并不断断裂的方式去除多余的界膜,形成体积较小、基质电子密度高、内含小泡排列紧密的高电子密度多泡体。因此,多泡体的形成既与内吞活动有关,又与高尔基体区域小泡有关。前多泡体和低电子密度多泡体不含溶酶体酶。在多泡体形成过程中,只有到高电子密度多泡体阶段,才与溶酶体发生关系,从溶酶体中获取溶酶体酶。多泡体形成后,常与自体吞噬泡靠近,可能参与睾丸间质细胞的自体吞噬活动。 相似文献
12.
Summary The jejunal absorptive cells of the salamander Amphiuma, when examined using transmission electron microscopy, were found to possess a unique type of intracellular vacuole containing membranous tubules. These vanoles, tentatively named multitubular bodies, were located in the cytoplasm between the nucleus and the brush-border membrane, and were seen with greatest frequency in the summer and fall. The vacuoles containing multitubular bodies had an average diameter of 0.6 m, and the membranous tubules within had an average diameter of 30 nm. The tubules differed morphologically from the vesicles in the multivesicular bodies, and from the primary lysosomes in the polylysosomal vacuoles. The tubules did not exhibit acid phosphatase activity, and were of similar diameter and membrane thickness as the Golgi saccules. In contrast to the multivesicular bodies, the multitubular bodies did not take up exogenous horseradish peroxidase. Early forms of autophagosomes resembling these vacuoles were often seen in the para-Golgi region of the cell. The multitubular bodies may represent a distinct type of autophagosome. Although the exact origin of the tubules as well as their role in cellular activity is unclear, their seasonal appearance within the multitubular bodies of the absorptive cells suggests a unique means of selective down-regulation of Golgi-like organelles. 相似文献
13.
Christiane Stang-Voss 《Cell and tissue research》1972,127(4):580-590
Zusammenfassung Im Laufe der Cytoplasmareduktion während der Spermiogenese von Eisenia foetida sind zwei Vorgänge zu unterscheiden: 1. Die Bildung autophagischer Vakuolen. Sie entstehen, indem Teile des Grundcytoplasmas in das Kompartiment des ER verlagert werden. Da sie keine Reaktion auf saure Phosphatase geben, sind sie als nicht lysosomale Anfangstadien der zellulären Autophagie zu betrachten. 2. Die Bildung primärer Lysosomen. Sie entstehen in Form von lytische Enzyme enthaltenden Golgivesikeln, die von einer neu im Cytoplasma entstehenden Membran zu größeren Einheiten zusammengefaßt werden: den multivesicular bodies. Autophagische Vakuolen und multivesicular bodies gelangen ins Cytophor das am Ende der Spermiogenese den Charakter eines ausgedehnten Autophagosoms annimmt. Als Struktureigentümlichkeit entstehen in ihm undulierende Tubulikörper. Der coat an den Hüllmembranen junger multivesicular bodies und am Plasmalemm der Spermatidenverbindung zum Cytophor wird in Zusammenhang mit der Membrandifferenzierung diskutiert.
Ultrastructural equivalents of cellular autophagyElectronmicroscopical observations on spermatids of Eisenia foetida during the cytoplasmic reduction
Summary During the cytoplasmic reduction phase in the spermiogenesis of Eisenia foetida two different processes may be defined: 1. The formation of autophagic vacuoles, which arise by the displacement of cytoplasmic portions into the cisternae of the endoplasmic reticulum. Since they exhibit no acid phosphatase activity they are considered to be early stages in cellular autophagy. 2. The formation of primary lysosomes. They originate in Golgi vesicles and are then enveloped by a membrane, formed in the cytoplasm de novo, which transforms them into multivesicular bodies. Autophagic vacuoles and multivesicular bodies subsequently transfer to the cytophor, which contains at the end of the spermiogenesis the characteristics of a large autophagosom, showing aggregates of undulating tubules. The outer coat of the limiting membranes in the early multivesicular bodies and of the cell membrane of the connecting piece between spermatid and cytophor appear to be associated with the membrane development.相似文献
14.
Summary Chromophilic cells in the proximal pars distalis of the adenohypophysis of Rhamdia hilarii were studied in thin section and freeze fracture preparations. The gonadotropic cells (GTH-cells) exhibit a diversity of form, the frequency of which can be related to stages (maturation, mature and spent) in the sexual cycle. GTH-cells showing a cytoplasm filled with electron dense polymorphic secretory granules and small rough endoplasmic reticulum (RER) vesicles, have been termed non-vacuolated. During the mature gonadal stage, such cells become increasingly vacuolated. The small RER vesicles become dilated and/or fuse, forming a single enormous cisternum (4–11 m diameter), the contents of which show direct contact with the inner nuclear membrane. These morphological aspects support the idea that Rhamdia hilarii possesses only one GTH-cell type. Evidence from freeze fracture replicas suggests that membrane-associated events precursory to exocytosis take place in regions where the cell and secretory granule membranes are in close apposition. Thin section analysis of secretory granule formation revealed their derivation from the dilated extremities of the inner Golgi saccule which appears to resemble the rigid lamella described in other cells. After detachment of the inner saccule, the immature secretory granules appear to enlarge by microvesicular transport. Freeze fracture and ultrastructural data on the morphology of the cells that presumably synthetise growth hormone are also presented.This work was aided by the Fundação de Amparo à Pesquisa do Estado de São Paulo (75/1282) 相似文献
15.
LYSOSOMES AND GERL IN NORMAL AND CHROMATOLYTIC NEURONS OF THE RAT GANGLION NODOSUM 总被引:28,自引:21,他引:7
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The rat ganglion nodosum was used to study chromatolysis following axon section. After fixation by aldehyde perfusion, frozen sections were incubated for enzyme activities used as markers for cytoplasmic organelles as follows: acid phosphatase for lysosomes and GERL (a Golgi-related region of smooth endoplasmic reticulum from which lysosomes appear to develop) (31–33); inosine diphosphatase for endoplasmic reticulum and Golgi apparatus; thiamine pyrophosphatase for Golgi apparatus; acetycholinesterase for Nissl substance (endoplasmic reticulum); NADH-tetra-Nitro BT reductase for mitochondria. All but the mitochondrial enzyme were studied by electron microscopy as well as light microscopy. In chromatolytic perikarya there occur disruption of the rough endoplasmic reticulum in the center of the cell and segregation of the remainder to the cell periphery. Golgi apparatus, GERL, mitochondria and lysosomes accumulate in the central region of the cell. GERL is prominent in both normal and operated perikarya. Electron microscopic images suggest that its smooth endoplasmic reticulum produces a variety of lysosomes in several ways: (a) coated vesicles that separate from the reticulum; (b) dense bodies that arise from focal areas dilated with granular or membranous material; (c) "multivesicular bodies" in which vesicles and other material are sequestered; (d) autophagic vacuoles containing endoplasmic reticulum and ribosomes, presumably derived from the Nissl material, and mitochondria. The number of autophagic vacuoles increases following operation. 相似文献
16.
W. Barry Piekos 《Cell and tissue research》1987,249(3):541-546
Summary Quantitative TEM refutes the hypothesis of multivesicular body formation at dawn via the degradation of photoreceptor. No significant relationship exists between multivesicular bodies and rhabdom diameter or between multivesicular bodies and coated vesicles. The density of multivesicular bodies is related to the vesicle-producing Golgi bodies. A new theory concerning the formation and possible function of multivesicular bodies is discussed. 相似文献
17.
The development of glyoxysomal marker enzyme activities and concomitant ultrastructural evidence for the ontogeny of glyoxysomes has been studied in cotyledons of dark-grown watermelon seedlings (Citrullus vulgaris Schrad., var. Florida Giant). Catalase (CAT, EC 1.11.1.6) was stained in glyoxysomal structures with the 3,3-diaminobenzidine procedure. Serial sections and high-voltage electron microscopy were used to analyze the three-dimensional structure of the glyoxysomal population. With early germination CAT was localized in three distinct cell structures: spherical microbodies already present in freshly imbibed cotyledons; in appendices on lipid bodies; and in small membrane vesicles between the lipid bodies. Due to their ribosome-binding capacity, both appendices and small vesicles were identified as derivatives of the endoplasmic reticulum (ER). In the following period, glyoxysome formation and lipid body degradation were found to be inseparable processes. The small CAT-containing vesicles attach to a lipid body on a restricted area. Both lipid body appendices and attached cisternae enlarge around and between tightly packed lipid bodies and eventually become pleomorphic glyoxysomes with lipid bodies entrapped into cavities. The close contact between lipid body and glyoxysomes is maintained until the lipid body is digested and the glyoxysomal cavity becomes filled with cytoplasm. During the entire period of increase in glyoxysomal enzyme activities, no evidence was obtained for destruction of glyoxysomes, but small CAT-containing vesicles were observed from day 2 through day 6 after imbibition, indicating a continuous de novo formation of glyoxysomes. This study does not substantiate the hypothesis that glyoxysomes bud directly from the ER. Rather, ER-derivatives, e.g., lipid body appendices or cisternae attached to lipid bodies are interpreted as being glyoxysomal precursors that grow in close contact with lipid bodies both in volume and surface membrane area.Abbreviations CAT
catalase
- DAB
3,3 diaminobenzidine tetrahydrochloride
- ER
endoplasmic reticulum
- GOX
glycolate oxidase
- HPR
hydroxypyruvate reductase
- HVEM
high-voltage electron microscopy
- ICL
isocitrate lyase
- MS
malate synthase
- RER
rough endoplasmic reticulum
In the figures bars represent 0.1 m (if not stated otherwise) 相似文献
18.
Summary Formation of melanosomes in melanophores of a teleost, Oryzias latipes, was studied by means of electron microscopy. Two distinct types of premelanosomes are observed in the same cell: (i) multivesicular premelanosomes, which later develop into melanosomes with electron-lucent hollows in the center, appear at early embryonic stages; (ii) premelanosomes with highly organized, fibrous internal structure are formed at later stages of development and give rise to melanosomes with a filamentous center. Melanosomes are generally ellipsoid in shape, and the difference in the dimensions of fibrillar premelanosomes, melanosomes in the cells at younger developmental stages and those developed fully in melanophores of adults indicates that these organelles grow during development. The growth is achieved by fusion of small unmelanized vesicles or fibrillar premelanosomes to preformed melanosome and by fusion of two or more premelanosomes to form a larger organelle. The addition of the matrix of fibrillar premelanosomes around preformed melanosomes, which are derived from either multivesicular or fibrillar premelanosomes, forms a concentric outer deposit, and the fusion of small vesicles produces electron-lucent pits which are scattered irregularly in mature melanosomes. 相似文献
19.
A cytochemical study of the Golgi apparatus of the spermatid during spermiogenesis in the rat 总被引:5,自引:0,他引:5
The reactivity of the various components of the Golgi apparatus of rat spermatids for three phosphatase activities (nicotinamide adenine dinucleotide phosphatase, NADPase; thiamine pyrophosphatase, TPPase; cytidine monophosphatase, CMPase) and the incorporation of 3H-fucose by the spermatids was analyzed at the 19 steps of spermiogenesis, i.e., during and after this organelle elaborated the glycoprotein-rich acrosomic system. During steps 1-3, the Golgi apparatus produced, in addition to the proacrosomic granules, multivesicular bodies that became associated with the chromatoid body. NADPase was located within the four of five intermediate saccules of Golgi stacks, and TPPase was found in the last one or two saccules on the trans aspect of the stacks from steps 1 to 17 of spermiogenesis. CMPase was located within the thick saccular GERL elements found in the trans region of the Golgi apparatus from steps 1 to 7 of spermiogenesis, but the CMPase-positive GERL disappeared from the Golgi apparatus after its detachment from the acrosomic system at step 8. Th acrosomic system itself was reactive from CMPase and TPPase but was negative for NADPase, while the multivesicular bodies were CMPase and NADPase positive but unreactive for TPPase. Tritiated-fucose was readily incorporated within the Golgi apparatus of steps 1-17 spermatids; in steps 1-7 it was subsequently incorporated within the acrosomic system and multivesicular bodies. These various data indicated (1) that the Golgi apparatus of spermatids, although it loses its CMPase-positive GERL element in step 8, retains evidence of functional capacity until it degenerates in step 17; (2) that in early spermatids the various saccular components of the Golgi are specialized with respect to enzymatic activities; and (3) that each Golgi region may contribute in a coordinated fashion to the formation of the acrosomic system and multivesicular bodies. 相似文献
20.
C. R. Lending 《Protoplasma》1996,195(1-4):68-77
Summary The seed storage proteins of maize (Zea mays L.) are synthesized during endosperm development on membrane-bound polyribosomes. Protein body formation in normal genotypes occurs via a sequential deposition of the various types of zeins, and leads to the formation of spherical structures with a diameter of about l m. In the endosperm mutantopaque-2 the level of one zein class is reduced; these kernels exhibit an opaque phenotype instead of the vitreous phenotype displayed in normal genotypes, presumably due to the decrease in total zein protein at the time of desiccation. Previous microscopic examination ofopaque-2 protein bodies at 22 DAP (days after pollination) showed that the protein bodies were morphologically similar to those of normal genotypes. However, the endosperm ofopaque-2 maize at 14 DAP contains tubular arrays within the rough endoplasmic reticulum. These tubular arrays are tightly associated with the developing protein bodies. Long strands of tubules, sometimes 10 m in length, are observed in the endosperm, and partially formed protein bodies often seem to be forming directly from these tubular arrays. No immunostaining is associated with this tubular material when any of the anti-zein antibodies are used.Abbreviations BSA
bovine serum albumin
- DAP
days after pollination
- IgG
immunoglobulin G
Dedicated to Professor Eldon H. Newcomb in recognition of his contributions to cell biology 相似文献