Relative water content and water potential of tissue 1

Relative water content (RWC) and water potential as measuredwith the pressure chamber were evaluated as indicators of waterstatus of tissue-cultured apple shoots and plantlets (shootswith roots). During the hydration required for RWC measurement,both water content and water potential exhibited the same hydrationkinetics, indicating that 10 h were required for full hydration.Once full hydration was reached, shoot mass remained relativelyconstant. Moisture release characteristics were also constructedand the associated shoot and plantlet water relations parameterswere estimated. Underin vitroconditions, both shoot and plantletwater potential were similar to the water potential of the culturemedium in which they were grown. The moisture release characteristicof shoots and plantlets was consistent with that expected fortypical plant tissues, and gave estimates of maximum modulusof elasticity (6.201.14 MPa), osmotic potential at saturation(–0.85 0.10 MPa), osmotic potential at zero turgor (–1.16 0.14 MPa) and RWC at zero turgor (78 2%) which were similarto values in the literature. Higher values of leaf conductanceand RWC were found in shoots and plantlets placed at 95% RH(21 C) compared to those at 90% RH. Plantlets had higher valuesof both conductance and RWC compared to shoots, suggesting thatinvitroroots are functional in water uptake. Relative water contentwas related to measures of physiological activity such as leafconductance, and it was also easier to measure than water potential.Relative water content is suggested as a sound index of waterstatus in tissue culture plants. Key words: Conductance, microculture, water status, water stress.     

Combined effect of virus infection and water stress on water flow and water economy in grapevines

Water limitation is one of the major threats affecting grapevine production. Thus, improving water‐use efficiency (WUE) is crucial for a sustainable viticulture industry in Mediterranean regions. Under field conditions, water stress (WS) is often combined with viral infections as those are present in major grape‐growing areas worldwide. Grapevine leafroll‐associated virus 3 (GLRaV‐3) is one of the most important viruses affecting grapevines. Indeed, the optimization of water use in a real context of virus infection is an important topic that needs to be understood. In this work, we have focused our attention on determining the interaction of biotic and abiotic stresses on WUE and hydraulic conductance (K_h) parameters in two white grapevine cultivars (Malvasia de Banyalbufar and Giró Ros). Under well‐watered (WW) conditions, virus infection provokes a strong reduction (P < 0.001) in K_petiole in both cultivars; however, K_leaf was only reduced in Malvasia de Banyalbufar. Moreover, the presence of virus also reduced whole‐plant hydraulic conductance (K_hplant) in 2013 and 2014 for Malvasia de Banyalbufar and in 2014 for Giró Ros. Thus, the effect of virus infection on water flow might explain the imposed stomatal limitation. Under WS conditions, the virus effect on K_plant was negligible, because of the bigger effect of WS than virus infection. Whole‐plant WUE (WUE_WP) was not affected by the presence of virus neither under WW nor under WS conditions, indicating that plants may adjust their physiology to counteract the virus infection by maintaining a tight stomatal control and by sustaining a balanced carbon change.     

Sedimentation of Bacillus sphaericus in tap water and sewage water

Formulations of Bacillus sphaericus products were applied in 1-m columns of clean water and sewage water in tubes. Samples taken through valves attached to the walls of the tubes showed that spores settled as a function of application method, droplet size, particle size, and water characteristics. In a mixture of deionized and tap water, the slowest sedimentation rate was obtained with a primary powder sonicated in water with 0.1% detergent. This product and a commercial fluid product settled faster in sewage water than in clean water and after 4 weeks, most spores were found in the sediment. In clean water, most spores remained suspended during the 4 weeks but the density was highest at the bottom. Calculation of total spore numbers in the columns after 4 weeks indicated that about 90% of spores disappeared in clean water, and 95-97% disappeared in sewage water. Bioassays showed that toxicity declined in parallel with the sedimentation, but at a higher rate, indicating a loss of toxicity from the residual spores.     

北京市水足迹及农业用水结构变化特征

运用水足迹的理论和方法计算评价了1990—2005年北京市水足迹及水资源利用的可持续性,在此基础上进一步分析了北京市农业用水结构的变化特征。结果表明:(1)北京市水足迹从1990年的81.5亿m3上升至2005年的168.6亿m3,人均水足迹由750.1m3上升为1096.0m3;(2)北京市水资源匮乏度不断升高,1995年以来水资源自给率呈下降趋势,与之相对应的水资源依赖度越来越高;(3)农业部门用水量在本地用水量中的比例平均每年为55.1%,虚拟水净输入量在虚拟水净输入总量中的比例平均每年达到89.1%;(4)高耗水型作物产品生产用水比例升高加大了农业用水压力,动物产品生产用水量呈增加趋势,2001—2005年动物产品生产引入的虚拟水占到其虚拟水总量的81.3%。北京市水资源利用呈不可持续状态,通过农业系统内部结构的优化调整,实现农业部门水资源的高效利用是缓解北京市水资源紧缺问题的关键。     

Self quenching of chlorosome chlorophylls in water and hexanol-saturated water

The optical properties of a methyl ester homolog of bacteriochlorophylld (BChld _M ) and bacteriochlorophyllc (BChlc) in H₂O, hexanol-saturated H₂O and methanol were studied by absorption, fluorescence emission, and circular dichroism (CD). In H₂O, BChld _M spontaneously forms an aggregate similar to that formed in hexane, with absorption maximum at 730 nm and fluorescence emission at 748 nm. For the pigment sample in hexanol-saturated H₂O, while the absorption peaks at 661 nm, only slightly red-shifted compared to the monomer, the fluorescence emission is highly quenched. When diluted 2–3 fold with H₂O, the absorption returns to around 720 nm, characteristic of an aggregate. The CD spectrum of the H₂O aggregate exhibits a derivative-shaped feature with positive and negative peaks, while the amplitude is lower than that of chlorosomes. The Fourier transform infrared spectra of BChld _M aggregates in H₂O and hexane were measured. A 1644 cm^–1 band, indicative of a bonded 13¹-keto group, is detected for both samples. A marker band for 5-coordinated Mg was observed at 1611 cm^–1 for the two samples as well. To study the kinetic behavior of the samples, both single-photon counting (SPC) fluorescence and transient absorption difference spectroscopic measurements were performed. For BChld _M in hexanol-saturated H₂O, a fast decay component with a lifetime of 10 to 14 ps was detected using the two different techniques. The fast decay could be explained by the concentration quenching phenomenon due to a high local pigment concentration. For the pigment sample in H₂O, SPC gave a 16 ps component, whereas global analysis of transient absorption data generated two fast components: 3.5 and 26 ps. The difference may arise from the different excitation intensities. With a much higher excitation in the latter measurements, other quenching processes, e.g. annihilation, might be introduced, giving the 3.5 ps component. Finally, atomic force microscopy was used to examine the ultrastructure of BChld _M in H₂O and hexanol-saturated H₂O. Pigment clusters with diameters ranging from 15 to 45 nm were observed in both samples.     

Analysis of pro/antioxidant properties of water and water solutions

The ability of water preparations of different composition to affect the generation of superoxide radicals has been compared. The superoxide-generating reaction of adrenaline autooxidation with some modifications was used as a model system, which makes it possible to reveal the pro/antioxidant properties of materials being tested. It was shown that samples of water from sources having different specific electroconductivity and, accordingly, ionic composition differ in the ability to affect reactions proceeding with the participation of ROS. The parameter measured, the pro/antioxidant activity of water, is a new informative indicator, and the approach proposed enables one to perform a comparative estimation of the quality of water and aqueous solutions.     

人工林地浑水入渗性能与通用入渗模型

采用双环法 ,通过 130场田间的浑水与清水入渗对比试验 ,对两种土壤质地的刺槐、侧柏人工林地的浑水入渗性能进行了研究。结果表明 ,含沙径流——浑水可显著削弱人工林地土壤的入渗性能 ,降低天然降水与土壤水的转换能力及人工林涵养水源的作用 ,其削减能力随着入渗水流含沙量、泥沙中小于 0 .0 1mm物理性粘粒含量的增加或入渗历时的延长而增大 ,并受到土壤质地的强烈影响。在土壤质地相同条件下 ,人工林地浑水的入渗能力随人工林树种的不同而异。刺槐林地土壤入渗能力大于侧柏林地 ;与相同立地退耕还林后仅 1a的新造林地相比较 ,退耕还林后 13a生的侧柏林地 ,土壤浑水入渗和清水入渗能力均减小 ;但退耕还林后 13a生的刺槐林地 ,清水入渗能力明显增强 ,浑水入渗能力因入渗水流特性不同而异。通过 L evenberg-Marquardt非线性参数拟合 ,求得了两种土壤质地条件下 ,3种林分积水型浑水与清水入渗的通用模型。该模型既可用于清水入渗预报 ,又可用于不同含沙量和泥沙粒度组成浑水入渗能力的预测     

The water content/water activity relationship of cured tobacco and water relations of associated spoilage fungi

The relationship between moisture content and water activity (a_w) in cured tobacco was significantly influenced by sugar content. Overall, high sugar tobaccos such as Oriental and Virginia had a higher moisture content at any given water activity compared to low sugar tobaccos such as Burley. Virginia and Burley were both predominantly colonised by Aspergillus and Penicillium spp. Of these, about 80% of isolates could germinate at between 0·75 and 0·85 a_w, equivalent to moisture contents of between 18% and 24% in Burley and between 22% and 31% in Virginia. Growth of the dominant Aspergillus and Penicillium spp. was much slower on Virginia and Burley tobacco extract than on malt extract agars over the range 0.85 to 0.98 a_w. For some species the optimum a_w for growth on tobacco extract medium was altered from that on the richer malt extract agar and for some there was also a significant difference in growth between Virginia and Burley extract agars. The mould-free storage periods for five different tobacco types was influenced by a_w. Visible moulding occurred within 7–14 days at 0.85–0.90 a_w but only after about six months at 0.70–0.75 a_w. There were some differences in rate of moulding between tobacco types as well as in the range of fungi isolated at different a_w storage levels.     

不同土壤水分条件下丹参耗水特征与水分利用率的研究

~~不同土壤水分条件下丹参耗水特征与水分利用率的研究@高扬$中国科学院、水利部水土保持研究所!陕西杨陵712100 @梁宗锁$中国科学院、水利部水土保持研究所!陕西杨陵712100;西北农林科技大学生命科学学院,陕西杨陵712100 @刘燕$陕西省药品监督管理局!西安710061~~~~[1] LIN J (林佳),XU L ZH(徐丽珍),LI Y(李琰), YANG SH L(杨世林). Comparison of tanshinone Ⅱ A content in the radix of Salvia miltiorrhiza Bge. from different producing area[J]. China Journal of Chinese Materia Medica(中国中药杂志), 2002.…     

Oxygen consumption rates of tilapia in fresh water, sea water, and hypersaline sea water

Whole animal oxygen consumption rates and plasma constituents were determined in the tilapia O. mossambicus , acclimated for 1 month in fresh water, sea water, and 1·6 × sea water. Oxygen consumption rates for the three water salinities were: 177·2 ± 16·86, 78·6 ± 2·32, and 195·4 ± 15·39 mg O₂ kg⁻¹ h⁻¹ (means ± 1 s.e.), respectively. Plasma prolactin (tPRL₁₈₈) concentration was significantly lower in 1·6 × sea water compared to fresh and sea water. There were no significant differences among mean plasma cortisol concentration and lysozyme activity. Ventilation was significantly higher in fish in sea water compared to the fish in fresh and 1·6 × sea water. The lowest oxygen consumption rates were found in fish acclimated to sea water. That salinity is probably closest to the brackish waters from which they were captured in the wild, and this agreement likely reflects the selection for optimal morphological and physiological characteristics to live in that environment.     

Diet of Rana cancrivora in fresh water and brackish water environments

Rana cancrivora Gravenhorst inhabits both fresh water and brackish water swamps and ditches in Singapore. Food items in the gut of frogs from both habitats have been examined. The diet of frogs collected near brackish water was predominantly crustacean and included crabs ( Sesarma spp.), while the diet of those collected near fresh water comprised mainly insects. Gut contents of frogs included all the small animal species found in the respective environments, the choice of prey appearing to be limited only by size.     

Basics of the molecular-ecological mechanism of water quality formation and water self-purification

The paper formulates some basics of the modern ecological theory of the polyfunctional role of biota in the molecular-ecological mechanism of water quality formation and self-purification of aquatic ecosystems. The theory covers the following items: (1) sources of energy for self-purification mechanisms, (2) the main structural and functional units of the self-purification system, (3) the main processes involved in the system, (4) contributions of major taxa to self-purification, (5) system reliability and supporting mechanisms, (6) the response of the system as a whole to external factors, (7) particulars of the operation of water purification mechanisms, and (8) conclusions and recommendations for biodiversity preservation practice.     

The turbulent boundary between water science and water management

SUMMARY. 1. It is common to observe friction between limnologists and the managers of water resources. This is often a result of misunderstandings about the cultures within which each works.
2. There are a number of ways that science can contribute to effective management of water resources, but limnologists must appreciate that there are value questions which are not the sole prerogative of science to answer.
3. Managers often misunderstand science and expect it to deliver a truth that is non-arguable. They fail to understand the very process of science demands no such truths, so that assumptions, methods and conclusions can always be challenged.
4. One way to bridge this boundary is to develop the scientific broking role. Another is to do better and more relevant science. Ways of doing both are discussed.     

Desalination and water reuse to address global water scarcity

The act of ensuring freshwater is considered the most essential and basic need for humanity. Although the planet is water-rich in some terms, the freshwater sources available for human consumption and beneficial uses are very limited. Excess population growth, industrial development coupled with improving living standards have caused an unprecedented need for freshwater all over the world. Regions once rich in water resources are struggling to meet the ever increasing demands in recent years. In addition, climate change and unsustainable management practices have led to a situation called “drought” in many regions. Water supplies in drought conditions can be addressed by taking two major approaches related to management and technology development. The management approaches include demand mitigation and supply enhancement. Demand mitigation can be done by implementing water conservation practices, and by enforcing a mechanism to influence user-responsible behavior through higher water fares and other billing routes. Supply enhancement can be achieved by utilizing the methods available for water reclamation, reuse and recycle including rain harvesting. This paper provides a critical insight of the causes for drought and the issues caused by persistent drought conditions followed by discussion of management and technological approaches required to maintain adequate water resources around the world. Challenges and opportunities involved in implementation of desalination and water reuse technologies in addressing global water scarcity are discussed in detail with case studies