共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
O S Gabrielsen N Marzouki A Ruet A Sentenac P Fromageot 《The Journal of biological chemistry》1989,264(13):7505-7511
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
AsanadaptermoleculebetweenmRNAandaminoacid,tRNAplaysacrucialintermediateroleinproteinbiosynthesis.TheinteractionbetweentRNAandaminoacyltRNAsynthetase(aaRS)istheprerequisiteforaccuratetranslation.Resultsofinvivoandinvitroexperimentsindicatethatthespecif… 相似文献
16.
Binding of different dimeric forms of PDGF to human fibroblasts: evidence for two separate receptor types 总被引:67,自引:8,他引:59
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
C H Heldin G B?ckstr?m A Ostman A Hammacher L R?nnstrand K Rubin M Nistér B Westermark 《The EMBO journal》1988,7(5):1387-1393
The binding of the three dimeric forms of platelet-derived growth factor (PDGF), PDGF-AA, PDGF-AB and PDGF-BB, to human fibroblasts was studied. Cross-competition experiments revealed the existence of two different PDGF receptor classes: the type A PDGF receptor bound all three dimeric forms of PDGF, whereas the type B PDGF receptor bound PDGF-BB with high affinity and PDGF-AB with lower affinity, but not PDGF-AA. The sizes of the two receptors were estimated with affinity labeling techniques; the A type receptor appeared as a major component of 125 kd and a minor of 160 kd, and the B type receptor as two components of 160 and 175 kd. A previously established PDGF receptor monoclonal antibody, PDGFR-B2, was shown to react with the B type receptor only. The different abilities of the three dimeric forms of PDGF to stimulate incorporation of [3H]TdR into human fibroblasts indicated that the major mitogenic effect of PDGF is mediated via the B type receptor. 相似文献
17.
The DnaX complex of the DNA polymerase holoenzyme assembles the beta(2) processivity factor onto the primed template enabling highly processive replication. The key ATPases within this complex are tau and gamma, alternative frameshift products of the dnaX gene. Of the five domains of tau, I-III are shared with gamma In vivo, gamma binds the auxiliary subunits deltadelta' and chipsi (Glover, B. P., and McHenry, C. S. (2000) J. Biol. Chem. 275, 3017-3020). To localize deltadelta' and chipsi binding domains within gamma domains I-III, we measured the binding of purified biotin-tagged DnaX proteins lacking specific domains to deltadelta' and chipsi by surface plasmon resonance. Fusion proteins containing either DnaX domains I-III or domains III-V bound deltadelta' and chipsi subunits. A DnaX protein only containing domains I and II did not bind deltadelta' or chipsi. The binding affinity of chipsi for DnaX domains I-III and domains III-V was the same as that of chipsi for full-length tau, indicating that domain III contained all structural elements required for chipsi binding. Domain III of tau also contained deltadelta' binding sites, although the interaction between deltadelta' and domains III-V of tau was 10-fold weaker than the interaction between deltadelta' and full length tau. The presence of both delta and chipsi strengthened the delta'-C(0)tau interaction by at least 15-fold. Domain III was the only domain common to all of tau fusion proteins whose interaction with delta' was enhanced in the presence of delta and chipsi. Thus, domain III of the DnaX proteins not only contains the deltadelta' and chipsi binding sites but also contains the elements required for the positive cooperative assembly of the DnaX complex. 相似文献
18.
Analysis of RNA polymerase III transcription complexes by gel filtration 总被引:35,自引:0,他引:35
19.