共查询到20条相似文献，搜索用时 15 毫秒
1.
2.
3.
4.
Two new species of black flies, Simulium (Gomphostilbia) roslihashimisp. n. and Simulium (Gomphostilbia) lurauensesp. n., are described on the basis of reared adult, pupal and larval specimens collected from Peninsular Malaysia. These two new species are placed in the ceylonicum species-group within the subgenus Gomphostilbia. Simulium (Gomphostilbia) roslihashimisp. n. is most distinctive with the male having almost entirely yellow antennae, and Simulium (Gomphostilbia) lurauensesp. n. is characterized in the female by having the elongate sensory vesicle and the yellowish-white hairs on the base of the costal vein and on the stem vein, in the male by the greater number of large upper-eye facets and the spindle-shaped hind basitarsi which are much narrower than the hind tibiae and femora and in the pupa by the small terminal hooks. Keys to species of the ceylonicum species-group reported from Peninsular Malaysia are provided for females, males, pupae and mature larvae. 相似文献
5.
昆虫共生菌及其代谢产物复杂多样,具有潜在的医药价值。一些可体外培养的昆虫共生菌活体菌株可用于临床加快伤口愈合和治疗肠易激综合征等。一些昆虫共生菌产生的有机酸、抗菌肽和生物碱能抑制人体病原菌,产生的脂肪酶、酵母毒素KT和活性氧能杀死疟原虫,产生的肽类、聚酮和酯类能抑制人体肿瘤。但是,受到功能菌株分离培养困难、临床试验不足和次级代谢产物引起的临床不良反应等方面的限制,共生菌在医药领域的应用仍处于初级阶段。本文对昆虫共生菌及其代谢产物的医药功能进行了综述,并分析了它们在医药领域的应用现状、存在的问题和解决途径,以期推进功能性共生菌的研究和应用。 相似文献
6.
DRION G. BOUCIAS JAMES J. BECNEL SUSAN E. WHITE MICHEAL BOTT 《The Journal of eukaryotic microbiology》2001,48(4):460-470
We describe the discovery and developmental features of a Helicosporidium sp. isolated from the black fly Simulium jonesi. Morphologically, the helicosporidia are characterized by a distinct cyst stage that encloses three ovoid cells and a single elongate filamentous cell. Bioassays have demonstrated that the cysts of this isolate infect various insect species, including the lepidopterans, Helicoverpa zea, Galleria mellonella, and Manduca sexta, and the dipterans, Musca domestica, Aedes taeniorhynchus, Anopheles albimanus, and An. quadrimaculatus. The cysts attach to the insect peritrophic matrix prior to dehiscence, which releases the filamentous cell and the three ovoid cells. The ovoid cells are short-lived in the insect gut with infection mediated by the penetration of the filamentous cell into the host. Furthermore, these filamentous cells are covered with projections that anchor them to the midgut lining. Unlike most entomopathogenic protozoa, this Helicosporidium sp. can be propagated in simple nutritional media under defined in vitro conditions, providing a system to conduct detailed analysis of the developmental biology of this poorly known taxon. The morphology and development of the in vitro produced cells are similar to that reported for the achorophyllic algae belonging to the genus Prototheca. 相似文献
7.
Neusa Hamada Vanderly Andrade-Souza Marcela De Paul Jeane Marcelle Cavalcante Nascimento 《Medical and veterinary entomology》2023,37(1):47-62
Problems related to the identity of Simuliidae species are impediments to effective disease control in Amazonia. Some of these species, such as Simulium oyapockense Floch & Abonnenc, 1946 (Diptera: Simuliidae), are vectors of the organisms that cause onchocerciasis and mansonellosis diseases. This blackfly species has a wide distribution in South America, and it is suspected of being a complex of cryptic species. The aim of this study is to characterize the nominal species S. oyapockense using partial COI gene sequences. Seven populations of S. oyapockense (morphologically identified) were analysed, including one from its type-locality. The other six populations were collected in Brazil and in Argentina. A taxon collected in Amazonas state, Brazil, with adults similar to S. oyapockense but with distinct pupae, was also included in the analysis (Simulium ‘S’). The nominal species S. oyapockense is circumscribed, and its geographical distribution is restricted to areas north of the Amazon River. Populations of S. oyapockense s.l. collected south of the Amazon River comprise a species complex that needs to be evaluated using integrative taxonomy. Simulium ‘S’ represents a species with unique morphological and molecular characteristics. Distinguishing cryptic species is a prerequisite for reducing the taxonomic impediment, especially in medically important taxa. 相似文献
8.
Joseph P. Iburg Elmer W. Gray Raymond Noblet 《Entomologia Experimentalis et Applicata》2016,159(1):70-76
Biologically based larvicides used to suppress nuisance populations of black flies (Diptera: Simuliidae) require that the larvae ingest the larvicide for mortality to occur. Materials present in the habitat that alter larval feeding behavior may reduce the effectiveness of the larvicide. This study was conducted to determine the effects that commonly occurring materials in larval habitats have on Simulium vittatum Zetterstedt feeding behavior. Larvae were exposed to 50 p.p.m. of kaolinite clay and 4 p.p.m. of purified diatom frustules. Larvae were also exposed to 10 000 cells or colonies per ml of viable green algae, Chlorella vulgaris Pratt and Scenedesmus quadricauda (Turp) Bréb, and the diatom Cyclotella meneghiniana Kützing to determine the effects that these materials have on larval feeding behavior. Individual larvae were recorded using a digital camcorder to determine the average number of times the larvae extended and retracted their cephalic fans per min (flick rate) in each medium. Following exposure to the selected material, neon red particles (NRP) (DayGlo) were added to the larval medium for 20 min. A spectrophotometric analysis was conducted to determine the average quantity of NRP consumed by larvae per min in each medium. The flick rate and quantity of NRP consumed by larvae was not significantly affected by clay material or viable Chlorella cells. When Scenedesmus colonies or Cyclotella cells were present in the larval medium, larval flick rate and NRP ingestion was significantly reduced. The presence of diatom frustules in the medium resulted in the most severe reduction in larval flick rate and NRP ingestion. The results suggest that larval flick rate can change depending on environmental conditions, and that suppression programs using biologically based larvicides may benefit from investigating the potential correlation between algal counts and treatment efficacy. 相似文献
9.
《Ethology, Ecology and Evolution》2012,24(4):363-377
The cocoon-spinning behaviour of black flies (Diptera Simuliidae) is a complicated innate behaviour. Despite this complexity, intraspecific variation in cocoon-spinning behaviour is low. To analyze the cocoon-spinning behaviour of black flies in a phylogenetic context the behaviour must be described, broken down into its constituent behavioural units and each of these units then used as a character for the phylogenetic analysis. This paper describes the cocoon-spinning behaviour of Simulium vittatum. Pre-spinning, spinning and post-spinning behaviour are analyzed. The pre-spinning behaviour involves the selection and cleaning of the pupation site. When spinning the cocoon, the pharate pupa (which outwardly resembles a larva) goes through six distinct stages (Front, S, Dorsal, Pull Front, Pull and Flip), all of which are described herein. The post-cocoon-spinning behaviour involves the pharate pupa breaking out of its larval skin and settling into the cocoon. The goals of this paper are to describe the cocoon-spinning behaviour for one species and establish terminology so that analyses of other black fly species in the future can focus on the character states to be used for phylogeny reconstruction. 相似文献
10.
Four species of adult herbivorous surgeonfishes (Family Acanthuridae) were collected from the remote South-Pacific island system of Tuvalu. Their intestinal contents were examined, and of four populations of ciliated protists, two new species were discovered and are described. Ciliates were examined after protargol staining and, in some cases, scanning electron microscopy. Members of each population were examined and 10 characters measured for the balantidia, and 13 for the paracichlidotherids. A new Balantidium is described which has an unusually large dextr-oral field of cilia. A new species of Paracichlidotherus was discovered which has a macronucleus significantly smaller and well anteriad the cytoplasmic portion of the oral polykinetids relative to the type species. 相似文献
11.
12.
Species richness and spatial distribution of blackflies (Diptera: Simuliidae) in streams of Central Amazonia, Brazil 总被引：2，自引：0，他引：2
1. The spatial distribution and species richness of blackflies were evaluated at 58 stream sites in Central Amazonia, Brazil. Samples were taken along a north–south axis of approximately 130 km and a east–west axis of approximately 220 km.
2. Based on stream-site characteristics, the occurrence of larvae of the six most frequently collected species was highly predictable (79.3–91.5% accuracy in prediction of occurrence). The predictive value of stream size and the presence of impoundments agrees with results of similar work in the Holarctic Region, suggesting a general responses of blackflies to environmental parameters.
3. Although only 19.0% of interstream variation in species richness was explained by a regression model, results suggested that species richness was greater in larger, cooler, faster, covered streams with rocky beds than in smaller, warmer, slower, open streams with sandy bottoms. Overall, the species richness of blackflies (11 species in total) was lower than in the temperate zone suggesting, for some taxa at least, that aquatic communities do not follow the terrestrial pattern of greater species richness in the tropics. 相似文献
2. Based on stream-site characteristics, the occurrence of larvae of the six most frequently collected species was highly predictable (79.3–91.5% accuracy in prediction of occurrence). The predictive value of stream size and the presence of impoundments agrees with results of similar work in the Holarctic Region, suggesting a general responses of blackflies to environmental parameters.
3. Although only 19.0% of interstream variation in species richness was explained by a regression model, results suggested that species richness was greater in larger, cooler, faster, covered streams with rocky beds than in smaller, warmer, slower, open streams with sandy bottoms. Overall, the species richness of blackflies (11 species in total) was lower than in the temperate zone suggesting, for some taxa at least, that aquatic communities do not follow the terrestrial pattern of greater species richness in the tropics. 相似文献
13.
The aquatic stages of the cattle biting pest, Simulium chutteri Lewis utilize river flow in their dispersal and colonization behaviour. Peaks of drifting activity in S. chutteri larvae occurred in the early morning and late afternoon. It is deduced that female flies scatter eggs onto slow flowing waters upstream of rapids. Larvae hatch from drifting eggs and colonize substrates in slower flowing regions upstream of rapids, while later stage instars move into faster flowing regions within rapids where they complete their development. This microhabitat selection by S. chutteri leads to rapid attainment of large population sizes in suitable sections of river and reduces competition between different stage larvae. In their utilization of a variety of microhabitats the larvae of this species differ from co-existing simuliid species which restrict developmental stages to single habitats.Catastrophic drift was recorded for S. chutteri larvae and could be a mechanism to regulate population size.Drift of simuliid larvae off rapids was not related to benthic population densities in the rapids and was therefore not due to excessive production. It is concluded that larval drift off rapids is related to habitat disturbances associated with water flow fluctuations and the activity of aquatic predators and other animals.The distribution of S. chutteri in the Vaal River is restricted by oviposition requirements of the adult female. Knowledge of drift behaviour and water flow requirements of Simuliidae have been applied to manage the population size of S. chutteri in the Vaal River. 相似文献
14.
PETER J. WANIEK LEILA MENDONÇA‐LIMA GUSTAVO B. MENEZES ANA M. JANSEN CATARINA A. C. ARAÚJO 《Physiological Entomology》2009,34(4):309-317
Insect c‐type lysozymes are antibacterial proteins that are synthesized in different organs with high activity against Gram‐positive bacteria. Because lysozymes possess muramidase activity, they also play an important role in the digestion of bacteria in Diptera. Triatomines express lysozyme‐encoding genes constitutively in the anterior region (cardia and stomach) of the midgut and the fat body after injection of bacteria into the haemocoel. The present study describes the overexpression of the Triatoma brasiliensis lysozyme 1 (lys1) in Escherichia coli. Recombinant T. brasiliensis Lys1 (TbLys1) is purified after solubilization of the inclusion bodies. The protein refolds successfully, showing muramidase activity against Micrococcus lysodeikticus lyophilized cells, after enterokinase cleavage of its thioredoxin fusion protein. In in‐gel zymograms and turbidimetric liquid assays TbLys1 is broadly active under alkaline and acid conditions, indicating a possible digestive function in the two physiologically different midgut regions of the bug: the stomach and small intestine. Muramidase activity is shown in the stomach and small intestine content of unfed bugs and bugs at different days after feeding, respectively. Western blot analysis identifies TbLys1 as lysozyme. 相似文献
15.
16.
W.A. Ramoska 《Journal of invertebrate pathology》1984,43(3):389-394
Beauveria bassiana conidia were bioassayed for pathogenicity against adult chinch bugs, Blissus leucopterus leucopterus, at varying percent relative humidities (RH). The conidia were found to be invasive and pathogenic at all humidities tested. Normal fungal replication and conidiogenesis, however, occurred only on the hosts incubated at the 75% or higher RH levels. 相似文献
17.
18.
19.