调控葡萄酒石酸生物合成VvbHLH79基因克隆及表达分析

【目的】葡萄是典型的酒石酸积累型果实,酒石酸不仅影响葡萄果实的味道,还决定葡萄酒的色泽、口感、微生物稳定性和陈酿潜力,但调控酒石酸生物合成的转录因子及机制尚不明确。【方法】bHLH转录因子参与调控植物的生长发育、次生代谢和抗逆性等多种生物学过程,课题组前期筛选到1条与酒石酸生物合成相关的bHLH转录因子。研究以酿酒葡萄‘琼瑶浆’、‘马瑟兰’为试验材料,采用PCR方法从中克隆到与酒石酸含量相关的VvbHLH79转录因子,对其进行生物信息学和亚细胞定位分析;利用HPLC和qRT-PCR技术测定葡萄果实在不同发育时期的酒石酸含量和VvbHLH79的表达量;并构建植物表达载体,使用农杆菌介导的果梗侵染法瞬时转化葡萄幼果果实之后检测VvbHLH79的表达量和酒石酸含量,以明确VvbHLH79基因在葡萄果实中对酒石酸含量的作用,为进一步探究VvbHLH79在调控葡萄酒石酸生物合成的分子机制奠定基础。【结果】结果表明：（1）通过RNA-seq测序筛选获得1个葡萄VvbHLH79基因,该基因CDS序列全长831 bp,编码277个氨基酸,相对分子量为66.22kD,理论等电点为5.15,属于无信号肽和跨膜结构的不稳定亲水性蛋白;氨基酸序列第147-232位含有bHLH-SF保守结构域,属于bHLH转录因子家族;亚细胞定位在细胞核。（2）同源序列比对和系统进化树结果显示,葡萄VvbHLH79编码蛋白与河岸葡萄中的bHLH89编码的蛋白（登录号：XP_034699340.1）序列一致性最高,达到99.64%。（3）qRT-PCR分析表明,VvbHLH79随着‘琼瑶浆’果实的发育,相对表达量在降低,从座果期到成熟期降低了67.25%左右;HPLC测定结果显示,酒石酸的积累主要在葡萄果实转色之前,转色之后酒石酸含量就快速下降。（4）VvbHLH79在低酒石酸葡萄果实中瞬时过表达后使酒石酸含量显著升高,而将VvbHLH79在高酒石酸葡萄果实中沉默后酒石酸含量显著下降,证实了VvbHLH79基因正向调控酒石酸含量。【结论】研究推测,VvbHLH79转录因子可能在葡萄酒石酸生物合成通路中发挥作用。     

辣椒果实中CaRIN转录因子的克隆及其表达分析

根据BLAST获得的表达序列标签设计引物,从辣椒中分离并鉴定了一个新型转录因子,命名为CaRIN。与已知MADS—box蛋白对比表明,CaRIN分别与番茄LeMADS－RIN和矮牵牛FBP4有85％和77％的同源性,并且具有典型的MADS—box类转录因子的特征区域。采用半定量RT－PCR进行特异性表达分析,结果表明CaRIN在破色期和红熟期辣椒果实中大量表达,而在绿熟期辣椒果实和其他辣椒器官中几乎不表达;同时进行了DNA结合位点和系统发生等生物信息学分析,这些结果都表明CaRIN可能参与辣椒果实成熟的调控。     

辣椒CaWRKY14转录因子的生物学特性研究

WRKY转录因子是植物响应病原菌胁迫最重要的转录因子之一,且参与抗病反应及信号传导通路的调控。为研究辣椒WRKY基因的生物学特征,以辣椒高抗疫病材料CM334为试材,克隆获得响应疫霉菌诱导的转录因子CaWRKY14。生物信息学分析表明,该基因DNA全长2 530 bp,cDNA全长1 662 bp,含有5个内含子,编码553个氨基酸,含有1个WRKY保守结构域,属于Group Ⅱ(b)。实时荧光定量表达分析表明,CaWRKY14不仅受ABA和疫霉菌胁迫诱导表达,且表达量分别在12 h和24 h时达到峰值,分别是对照的8.54和8.04倍,同时也受高盐、热激和干旱胁迫诱导。利用VIGS技术对CaWRKY14转录因子进行沉默后发现,抗病材料CM334接种疫霉菌后趋于发病。研究表明,CaWRKY14基因在辣椒响应疫霉菌胁迫进程中可能发挥着重要作用。     

蒺藜苜蓿MtbHLH25基因克隆、亚细胞定位及表达分析

【目的】bHLH转录因子数量众多,能够广泛参与植物的生长发育和逆境胁迫等过程。本试验以蒺藜苜蓿R108为材料,初步探讨MtbHLH25基因的功能。【方法】通过PCR扩增技术从蒺藜苜蓿中克隆MtbHLH25基因和启动子,构建酵母表达载体并用LiAc转化法转移到Y2H Gold酵母菌株中进行酵母自激活检测,构建亚细胞定位载体并通过冻融法转入农杆菌EHA105,菌液注射到烟草下表皮细胞后利用SP8激光共聚焦显微镜观察,通过实时荧光定量PCR技术研究MtbHLH25基因的时空表达水平。【结果】（1）从蒺藜苜蓿中成功克隆出MtbHLH25基因和启动子,该基因总长882 bp,共编码293个氨基酸。启动子序列分析发现其包含了ABA、MeJA、GA和SA等响应元件。（2）进化树结果表明MtbHLH25蛋白与蚕豆和长柔毛野豌豆中bHLH蛋白高度同源。（3）亚细胞定位结果显示MtbHLH25蛋白定位于细胞核。（4）酵母自激活检测结果显示MtbHLH25蛋白具有自激活活性。（5）表达分析结果显示,MtbHLH25在蒺藜苜蓿根、茎、叶、花和果实中均有表达,其中在根中表达水平最高;外源SA、MeJA、ABA、GA以及盐胁迫使MtbHLH25基因表达量都呈下降趋势,推测SA、MeJA、ABA、GA以及盐胁迫对MtbHLH25基因的表达起到负调控作用。干旱胁迫能够显著诱导MtbHLH25基因表达量的上升,说明该转录因子可能在干旱胁迫中起到正调控作用。【结论】MtbHLH25基因可能对盐胁迫敏感,在干旱胁迫中可能发挥正调控作用。此外,MtbHLH25蛋白具有自激活活性,对下游启动子调控的报告基因可能具有激活作用。     

鼠疫菌H-NS蛋白的表达与纯化及其DNA结合活性

摘要：【目的】利用大肠杆菌BL21λDE3的表达系统,表达出有活性的鼠疫耶尔森氏菌（以下简称鼠疫菌）调控子蛋白H-NS,为进一步研究H-NS的转录调控奠定基础。【方法】 PCR扩增鼠疫菌201株hns基因的编码区,将其直接克隆入pET28a质粒中,再将pET28a-hns重组质粒转入大肠杆菌BL21λDE3菌株中,所得菌株经IPTG诱导后能表达出鼠疫菌His-H-NS蛋白;通过体外的凝胶迁移实验（EMSA）和DNaseⅠ足迹实验对His-H-NS蛋白与DNA的结合活性进行分析。【结果】成功表达出有活性的鼠疫菌His-H-NS蛋白,该蛋白对鼠疫菌pH6抗原基因（psaA、psaE）及rovA基因均有结合活性。【结论】鼠疫菌His-H-NS具有DNA结合活性,说明H-NS能调控鼠疫菌基因的转录。     

MADS-box转录因子编码基因Vvrin1在草菇不同发育时期的表达分析

草菇是我国土特产出口的主要种类之一,而采后易开伞问题限制了草菇的贮藏及运输。MADS-box转录因子对植物的成熟衰老和真菌的子实体发育起到重要的调控作用。目前尚未见草菇MADS-box转录因子的相关报道。本研究通过生物信息方法及分子生物学的手段对草菇的基因组、转录组数据进行分析,获得了草菇MADS-box转录因子基因Vvrin1。该基因全长1 392bp,含2个内含子,编码419个氨基酸残基。该转录因子含有一个MADS-box结构域,序列与双孢蘑菇Agaricus bisporus、斑玉蕈Hypsizygus marmoreus和灰盖鬼伞Coprinopsis cinerea的MADS-box转录因子相似性分别为71%、67%和61%。通过表达谱数据及荧光定量PCR分析表明Vvrin1基因在草菇子实体伸长期菌柄的表达量出现高峰,具有极显著性差异（P<0.01）,推测该转录因子参与调控草菇菌柄的伸长,菌盖的开伞。这些结果为草菇成熟衰老（特别是开伞）的调控研究提供数据支持。     

苦荞麦FtWRKY28基因克隆及其在低磷与激素诱导下的表达分析

【目的】WRKY转录因子参与植物对低磷胁迫反应的调控。文章基于前期苦荞在低磷胁迫下的转录组数据,克隆FtWRKY28 基因,预测基因及其编码蛋白的序列结构特征,分析基因在各器官中以及在低磷和激素处理下的表达模式,分析蛋白的亚细胞定位与转录激活活性,为阐明基因的生物学功能奠定基础。【方法】基于苦荞基因组数据库注释设计特异引物,用逆转录PCR从低磷处理的苦荞根RNA样中克隆FtWRKY28的完整编码序列,采用生物信息学方法分析基因与蛋白的结构以及同源蛋白的进化关系,采用实时荧光定量PCR(qRT-PCR)分析基因的表达模式,采用拟南芥原生质体瞬时表达体系分析蛋白的亚细胞定位,采用酵母单杂交分析蛋白的转录激活活性。【结果】FtWRKY28的完整编码序列长876 bp,编码1个含291个氨基酸、有1个WRKY结构域、锌指结构域为C2H2型的蛋白,归属WRKY家族Ⅱ组。FtWRKY28定位于细胞核,具有转录激活活性。FtWRKY28在根中表达水平最高,受低磷、吲哚乙酸、赤霉素3和6-苄氨基嘌呤显著诱导。【结论】FtWRKY28具有转录因子的基本结构与生物化学特征,可能通过生长素、赤霉素、细胞分裂素信号网络的交互作用,调控苦荞在低磷胁迫下的响应过程。     

MADS-box转录因子的相互作用及对果实发育和成熟的调控

MADS-box基因编码的蛋白是一类数目庞大的转录因子家族, 通过与其他转录因子相互作用形成同源或异源二聚体, 调控着整个植株的生长发育。文章对近年来MADS-box转录因子的相互作用及对果实发育和成熟的调控作用的研究进展进行了综述, 为了解MADS-box转录因子的作用方式和作用机理及深入研究MADS-box基因对调控果实发育和成熟的作用提供参考。     

棉花MADS-box蛋白基因(GhMADS-13)的克隆和表达分析

一组具有MADS-box结构域的转录因子在控制花器官的诱导与发育中起着重要作,以中棉所36(CCRI)均一化全长cDNA文库为基础,结合EST测序分析,分离获得一个棉花的MADS-box基因全长cDNA.该基因cDNA全长1 079 bp,包含一个732 bp的完整的开放阅读框,编码234个氨基酸,具有典型的MADS-box结构域和完整的K区,命名为GhMADS-13(GenBank:FJ409870).与拟南芥、葡萄等植物的AGL6类基因具有高度的同源性.实时定量RT-PCR分析表明,GhMADS-13在CCR136的花器官发育中早期表达量逐步增加,后期有下降趋势.推测GhMADS-13可能在开花诱导和花器官发育过程中起着重要作用.     

菠萝花发育相关基因AcMADS1的克隆与组织表达特性分析

MADS-box转录因子在植物的发育过程特别是控制花器官的诱导与发育中起关键作用。利用同源克隆结合RACE技术, 从菠萝(Ananas comosus)花中分离出1个新的菠萝MADS-box基因, 命名为AcMADS1(GenBank登录号为KC257408)。AcMADS1基因的编码区为726 bp, 编码241个氨基酸, 蛋白质分子量为27.50 kDa, 等电点为9.26。序列比对和系统进化树分析表明, AcMADS1具有保守的MADS-box及半保守的K区, 属于AGL6亚家族MADS-box蛋白。生物信息学分析表明, AcMADS1是亲水碱性蛋白, 二级结构主要以α-螺旋、无规则卷曲和折叠延伸链为蛋白质骨架, 三级结构中蛋白核心结构符合转录因子与DNA结合的常见功能域MADS-box, 而且作为转录因子定位于细胞核中。组织特异性表达分析表明, AcMADS1基因在菠萝果肉以及花器官的雌蕊、花瓣和萼片中均有表达, 但在雄蕊以及营养器官的根、茎和叶中几乎不表达; 且在花器官早期发育过程中大量表达, 后期呈下降趋势。因此推测这个基因可能在菠萝花器官发育和开花诱导过程中起重要作用。     

Distribution of N,P, K,Ca, Mg,S, Zn,Fe, Mn and Cu in groundnut

Summary Concentration of N, P, K, Ca, Mg and S in summer groundnut crop was higher than in kharif while Zn, Fe, Mn and Cu contents were higher in summer crop. Kernel's N, P and Zn; Leaflet's Ca and Mn; Stem's K and Fe; Root's S and Cu and Petiole's Mg contents were highest. Shell's N, P, K, Mg, S, Zn and Cu; Kernel's Ca, Fe and Mn contents were the least. N, P, K, S, Zn and Cu concentrations decreased linearly as the crop grew. Ca, Mg, Fe and Mn concentrations did not display any distinct pattern. Ca concentration was positively correlated with pod yield in both the seasons.     

Simultaneous measurement of the trace elements Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum and their reference ranges by ICP-MS

The goal of this article was to establish reference ranges of the concentration of trace elements in human serum and to compare these results with those reported by other authors. We describe the sample preparation and measurement conditions that allow the rapid, precise, and accurate determination of Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum samples (n=110) by inductively coupled plasma-mass spectrometry (ICP-MS). Accuracy and precision were determined by analyzing three reconstituted reference serum samples by comparison with other methods and by the standard addition procedure. The advantages of the ICP-MS method include short time of analysis of the elements mentioned, low detection limit, high precision, and high accuracy. Disadventages include a high risk of contamination due to the presence of some of the elements of interest in the environment, the relatively delicate sample handling, and the high cost of the equipment.     

Reference values for the concentrations of As,Cd, Co,Cr, Cu,Fe, I,Hg, Mn,Mo, Ni,Pb, Se,and Zn in selected human tissues and body fluids

This report attempts to formulate reference ranges of elemental concentrations for 15 trace elements in selected human tissues and body fluids. A set of samples consisting of whole blood, blood serum, urine, milk, liver, and hair were chosen and considered for 15 elements of biological significance: As, Cd, Co, Cr, Cu, F, Fe, I, Hg, Mn, Mo, Ni, Pb, Se, and Zn. The results represent wholly or partially data received from 40 countries of the global regions of Africa, Asia, Europe, North, South, and Central America, Australia, and New Zealand. This survey, even if qualitative, has been useful in demonstrating certain trends of trace-element scenarios around the world. It is of course recognized that both diet and environment exert a strong influence on the distribution pattern of several elements, such as As, Cd, Mn, Pb, Se, and Zn. A limited comparison of the available information on soil status of different countries reflected some interesting associations for elements, such as Mn and Zn. Importantly, this study revealed that only a few countries were in a position to identify a reasonable amount of data on samples requested for this project. Regretably, for a number of countries, any dependable data for even such essential elements as Cu, Fe, and Zn were not available. In view of the nutritional importance of many elements, the time is ripe for concerted efforts by intergovernmental agencies to initiate investigations or commission task forces/projects to generate reliable reference data for selected global regions, which sadly lack data of any kind at present.     

Monovalent Cation Permeation through the Connexin40 Gap Junction Channel : Cs,Rb, K,Na, Li,TEA, TMA,TBA, and Effects of Anions Br,Cl, F,Acetate, Aspartate,Glutamate, and NO3

The unitary conductances and permeability sequences of the rat connexin40 (rCx40) gap junction channels to seven monovalent cations and anions were studied in rCx40-transfected neuroblastoma 2A (N2A) cell pairs using the dual whole cell recording technique. Chloride salt cation substitutions (115 mM principal salt) resulted in the following junctional maximal single channel current-voltage relationship slope conductances (γ_j in pS): CsCl (153), RbCl (148), KCl (142), NaCl (115), LiCl (86), TMACl (71), TEACl (63). Reversible block of the rCx40 channel was observed with TBA. Potassium anion salt γ_j are: Kglutamate (160), Kacetate (160), Kaspartate (158), KNO₃ (157), KF (148), KCl (142), and KBr (132). Ion selectivity was verified by measuring reversal potentials for current in rCx40 gap junction channels with asymmetric salt solutions in the two electrodes and using the Goldman-Hodgkin-Katz equation to calculate relative permeabilities. The permeabilities relative to Li⁺ are: Cs⁺ (1.38), Rb⁺ (1.32), K⁺ (1.31), Na⁺ (1.16), TMA⁺ (0.53), TEA⁺ (0.45), TBA⁺ (0.03), Cl⁻ (0.19), glutamate⁻ (0.04), and NO₃− (0.14), assuming that the monovalent anions permeate the channel by forming ion pairs with permeant monovalent cations within the pore thereby causing proportionate decreases in the channel conductance. This hypothesis can account for why the predicted increasing conductances with increasing ion mobilities in an essentially aqueous channel were not observed for anions in the rCx40 channel. The rCx40 effective channel radius is estimated to be 6.6 Å from a theoretical fit of the relationship of relative permeability and cation radius.     

Crystal structure, detonation performance, and thermal stability of a new polynitro cage compound: 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptacyclo [5.5.1.13, 11. 15, 9] pentadecane

A new polynitro cage compound 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptcyclo [5.5.1.1(3,11).1(5,9)] pentadecane (NNNAHP) was designed in the present work. Its molecular structure was optimized at the B3LYP/6-31 G(d,p) level of density functional theory (DFT) and crystal structure was predicted using the Compass and Dreiding force fields and refined by DFT GGA-RPBE method. The obtained crystal structure of NNNAHP belongs to the P-1 space group and the lattice parameters are a = 9.99 ?, b = 10.78 ?, c = 9.99 ?, α = 90.01°, β = 120.01°, γ = 90.00°, and Z = 2, respectively. Based on the optimized crystal structure, the band gap, density of state, thermodynamic properties, infrared spectrum, strain energy, detonation characteristics, and thermal stability were predicted. Calculation results show that NNNAHP has detonation properties close to those of CL-20 and is a high energy density compound with moderate stability.     

Reliability of Analyses of Hg,Fe, Ca,K, P,pH, Alkalinity,Conductivity, Hardness and Colour from Lakes

The aim of this report has been to present results concerning analytical quality controls of Hg analysis of fish and sediment, analyses of Fe, Ca, total-P, K, pH, alkalinity, conductivity, colour and hardness (Ca + Mg) of lake water samples. Despite the fact that these are standard parameters in many regular water control programs, there are major differences in the reliability with which these parameters can be determined. The focus here is on an overall inter-laboratory comparison between the parameters. Six laboratories have been involved in the analysis. Selected results: pH gives the lowest (average) relative standard deviation (error), about 2 %; conductivity gives an error of about 5–7 %; alkalinity yields an average error of as much as 13–25 %, which is the largest among the parameters studied here; colour also gives a high error, 9–15 %; hardness gives a relative standard deviation of about 6–7 %. Of the other parameters (i. e., Hg, Fe, Ca and P), Hg gives the best reliability and Fe and P the lowest. To have knowledge of the reliability of the analytical data is of paramount importance in most control programs and research projects.     

Competitive inhibition and selectivity enhancement by Ca in the uptake of inorganic elements (Be, Na, Mg, K, Ca, Sc, Mn, Co, Zn, Se, Rb, Sr, Y, Zr, Ce, Pm, Gd, Hf) by carrot (Daucus carota cv. U.S. harumakigosun)

We investigated the uptake of inorganic elements (Be, Na, Mg, K, Ca, Sc, Mn, Co, Zn, Se, Rb, Sr, Y, Zr, Ce, Pm, Gd, and Hf) and the effect of Ca on their uptake in carrots (Daucus carota cv. U.S. harumakigosun) by the radioactive multitracer technique. The experimental results suggested that Na, Mg, K, and Rb competed for the functional groups outside the cells in roots with Ca but not for the transporter-binding sites on the plasma membrrane of the root cortex cells. In contrast, Y, Ce, Pm, and Gd competed with Ca for the transporters on the plasma membrane. The selectivity, which was defined as the value obtained by dividing the concentration ratio of an elemental pair, K/Na, Rb/Na, Be/Sr, and Mg/Sr, in the presence of 0.2 and 2 ppm Ca by that of the corresponding elemental pair in the absence of Ca in the solution was estimated. The selectivity of K and Rb in roots was increased in the presence of Ca. The selectivity of Be in roots was not affected, whereas the selectivity of Mg was increased by Ca. These observations suggest that the presence of Ca in the uptake solution enhances the selectivity in the uptake of metabolically important elements against unwanted elements.     

Relations of the Al,B, Ba,Br, Ca,Cl, Cu,Fe, K,Li, Mg,Mn, Na,P, S,Si, Sr,and Zn mass fractions to morphometric parameters in pediatric and nonhyperplastic young adult prostate glands

The variation with age of the 18 trace element mass fractions and some histological characteristics of intact prostate glands of 50 subjects aged 0–30 years was investigated by instrumental neutron activation analysis, inductively coupled plasma atomic emission spectrometry, and a quantitative morphometric analysis. Mean values ± standard error of the mean (M ± SΕΜ) for the mass fractions (in milligrams per kilogram wet tissue) of these trace elements in pre-puberty were: Al 28.5 ± 9.0, B 0.40 ± 0.11, Ba 1.48 ± 0.44, Br 10.5 ± 1.5, Ca 241 ± 30, Cl 3,203 ± 278, Cu 3.51 ± 0.89, Fe 33.7 ± 4.1, K 2,364 ± 145, Li 0.020 ± 0.004, Mg 153 ± 23, Mn 0.46 ± 0.06, Na 2,286 ± 130, P 1,391 ± 100, S 1,698 ± 132, Si 62 ± 11, Sr 0.38 ± 0.08, and Zn 27.6 ± 2.3. During puberty and postpuberty, when there is a significant increase in circulating androgens, the mean values were: Al 7.2 ± 1.4, B 0.21 ± 0.05, Ba 0.25 ± 0.06, Br 5.8 ± 1.0, Ca 433 ± 81, Cl 2,314 ± 201, Cu 1.77 ± 0.13, Fe 20.9 ± 1.6, K 2,585 ± 118, Li 0.0088 ± 0.0014, Mg 232 ± 27, Mn 0.34 ± 0.04, Na 1,875 ± 107, P 1,403 ± 98, S 1,673 ± 73, Si 22.2 ± 3.1, Sr 0.22 ± 0.03, and Zn 93.3 ± 8.9. Mean values (M ± SΕΜ) of percent volumes (%) of the stroma, epithelium and lumen in the prostate before puberty were 73.4 ± 2.6, 20.4 ± 1.7, and 4.45 ± 0.94, respectively, versus 46.5 ± 2.5, 38.5 ± 1.9, and 14.9 ± 1.2 during puberty and postpuberty. This work’s results confirm that the Zn mass fraction in prostate tissue is an androgen-dependent parameter. For the first time it has been demonstrated that the glandular lumen is a main pool of Ca, Mg, and Zn accumulation and that the stroma is a main pool of Al, B, Ba, Br, Cl, Cu, Fe, Mn, Na, and Si accumulation in the normal human prostate, for the age range 0–30 years. It was concluded that the Ca, Mg, and Zn binds tightly within the prostatic fluid, because the volume of glandular lumen reflects the volume of prostatic fluid.     

The Effect of Age and Gender on Al, B, Ba, Ca, Cu, Fe, K, Li, Mg, Mn, Na, P, S, Sr, V, and Zn Contents in Rib Bone of Healthy Humans

The effect of age and gender on major, minor, and trace element contents in the intact rib bone of 80 relatively healthy 15–55-year-old women and men was investigated. Contents or upper limit of contents of 16 chemical elements in the rib bone were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). Mean values (M?±?SΕΜ) for the mass fraction of Ba, Ca, Cu, Fe, K, Li, Mg, Na, P, S, Sr, and Zn (milligram per kilogram of dry bone) were as follows: 2.54?±?0.16, 171,400?±?4,050, 1.35?±?0.22, 140?±?11, 1,874?±?71, 0.049?±?0.011, 2,139?±?38, 5,378?±?88, 75,140?±?1,660, 1,881?±?51, 291?±?20, and 92.8?±?1.5, respectively. The upper limits of contents of Al, B, Mn, and V were <7.20, <0.65, <0.36, and <0.03, respectively. Statistically significant tendency for the Ca, Mg, and P content to decrease with age was found in the human rib bone, regardless of gender. The mass fraction of Fe in the male rib bone increases with age. It was shown that higher Ca, Mg, Na, P, and Sr mass fractions as well as lower Fe content were typical of female ribs as compared to those in male ribs.