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1.
Assessment of gene flow from glyphosate-resistant transgenic soybean to conventional soybean in China 总被引:1,自引:0,他引:1
Wen-Kun Huang Huan Peng Gao-Feng Wang Jiang-Kuan Cui Lin-Feng Zhu Hai-Bo Long De-Liang Peng 《Acta Physiologiae Plantarum》2014,36(7):1637-1647
Glyphosate-resistant (GR) transgenic soybean has never been cultivated commercially in China. It is essential to develop the separation measures required to prevent out-crossing between GR and conventional soybean (Glycine max (L.) Merr.) by characterizing the transgene flow before GR soybean is released. In this study, the transgene flow from a GR transgenic soybean AG5601 to conventional soybeans was characterized. First, natural out-crossing rate was evaluated using 36 conventional soybean varieties interplanted with GR soybean AG5601 transformed with a cp4 EPSPS gene conferring the resistance to herbicide glyphosate in the field in 2007 and 2008 in China. Second, drift distance of cp4 EPSPS gene from GR soybean AG5601 to soybean cv. Zhonghuang13 was evaluated using the progenies harvested from eight directions at different distance. Third, the relationship of gene flow of GR soybean AG5601 with flowering synchronization days or insect pollinators of each variety was analyzed using regression analysis. Thirty-two of 36 tested conventional soybean varieties had surviving progenies after two times of sprays of glyphosate, and 49 of 41,679 progenies were verified to be glyphosate-tolerant heterozygous offspring. The out-crossing rates in positive varieties (having surviving offspring after two times of sprays of glyphosate) ranged from 0.039 to 0.934 %. The farthest distance (drift distance) between soybean AG5601 and cv. Zhonghuang13 at which out-pollinating was still able to be observed was 15 m, with an out-crossing rate of 0.012 %. Regression analysis showed that there was a positive relationship between cross-pollination frequency and flowering synchronization days or pollinator insects. Therefore, when GR soybean is released to the field, it should be critically separated with the conventional soybean in space and cultivation time with efficient insect control during flowering. 相似文献
2.
Evaluation of transgenic cotton varieties and a glyphosate application on seedling disease incidence
A study was conducted to determine whether stand densities of transgenic cotton (Gossypium hirsutum) varieties, with or without glyphosate, were similar to conventional varieties of the same lineage group in Georgia and Mississippi.
Transgenic and conventional cotton varieties were placed into five lineage groups of related varieties and seedling disease
was evaluated in three greenhouse tests and a field trial using Rhizoctonia solani AG-4. Seed vigor was determined by standard germination studies were conducted evaluating conventional and transgenic varieties
of similar lineage. Results showed that no interactions occurred for the heights and dry weight data across treatments within
the lineage groups in any of the experiments. No interactions were shown between stand densities at different inoculum rates
and inoculated versus uninoculated pots (plots). Across all greenhouse studies, stand counts of PM 1220 were similar to the
transgenic varieties PM 1220 RR and PM 1220 B/RR with or without a glyphosate application. In the field trial, PM 1220 B/RR
+ glyphosate had significantly lower stands than all other treatments expect PM 1220 RR (no glyphosate treatment) prior to
and after glyphosate application. Stand densities for varieties within the lineage group DPL 5415 were also inconsistent when
compared between the greenhouse and field trials with no apparent trends occurring. However, the Coker 312 varieties containing
glyphosate tolerance had consistently lower stand counts compared to the conventional variety of Coker 312 but only during
the greenhouse studies. Seed germination of Coker 312 could not be correlated with either the greenhouse or field trial data.
In general, the commercially available varieties such as PM 1220, DPL 5690, DPL 5415, and DPL 50 with glyphosate tolerance
had similar stand count, height, and dry weight data when compared to the conventional varieties from the same lineage group
regardless of a glyphosate application. When differences did occur, no consistent trends could be determined within these
four lineage groups.
An erratum to this article is available at . 相似文献
3.
Evaluation of transgenic cotton varieties and a glyphosate application on seedling disease incidence
A study was conducted to determine whether stand densities of transgenic cotton (Gossypium hirsutum) varieties, with or without glyphosate, were similar to conventional varieties of the same lineage group in Georgia and Mississippi. Transgenic and conventional cotton varieties were placed into five lineage groups of related varieties and seedling disease was evaluated in three greenhouse tests and a field trial using Rhizoctonia solani AG-4. Seed vigor was determined by standard germination studies were conducted evaluating conventional and transgenic varieties of similar lineage. Results showed that no interactions occurred for the heights and dry weight data across treatments within the lineage groups in any of the experiments. No interactions were shown between stand densities at different inoculum rates and inoculated versus uninoculated pots (plots). Across all greenhouse studies, stand counts of PM 1220 were similar to the transgenic varieties PM 1220 RR and PM 1220 B/RR with or without a glyphosate application. In the field trial, PM 1220 B/RR + glyphosate had significantly lower stands than all other treatments expect PM 1220 RR (no glyphosate treatment) prior to and after glyphosate application. Stand densities for varieties within the lineage group DPL 5415 were also inconsistent when compared between the greenhouse and field trials with no apparent trends occurring. However, the Coker 312 varieties containing glyphosate tolerance had consistently lower stand counts compared to the conventional variety of Coker 312 but only during the greenhouse studies. Seed germination of Coker 312 could not be correlated with either the greenhouse or field trial data. In general, the commercially available varieties such as PM 1220, DPL 5690, DPL 5415, and DPL 50 with glyphosate tolerance had similar stand count, height, and dry weight data when compared to the conventional varieties from the same lineage group regardless of a glyphosate application. When differences did occur, no consistent trends could be determined within these four lineage groups. 相似文献
4.
We used sets of meta-analyses to review the evidence of sex-biased herbivory in dioecious plants as well as intersexual differences in plant characteristics that might affect herbivores. Thirtythree studies were reviewed to assess the effects of plant sex on herbivore abundance, survivorship and damage imposed to host plants, whereas 54 studies were reviewed for the effects of plant sex on plant morphological, physiological, and nutritional characteristics. The standardized mean difference between males and females was chosen as the measure to calculate effect sizes. Male plants exhibited significantly higher numbers of herbivores (d++=1.074) and significantly higher herbivory measured in terms of plant damage (d++=0.577) compared to female plants. Effects of plant sex on herbivore abundance were stronger for folivores and gallformers compared to the other guilds, whereas effects of plant sex on herbivore damage were stronger for flower predators compared to browsers and folivores. No difference in herbivore survivorship was observed between sexes. Male plants exhibited significantly more leaves (d++=0.202), larger leaves (d++=0.91), fewer flowers (d++=−0.89) and longer stems (d++=0.614) than female plants. Although male plants exhibited significantly lower concentrations of secondary compounds (d++=−0.209) and other defenses (d++=−0.53) than female plants, no difference in nutrient concentration, such as foliar nitrogen, was observed between sexes. 相似文献
5.
干旱胁迫对大豆生长的影响及抗旱性评价方法与指标筛选 总被引:1,自引:0,他引:1
2014-2015年在年降雨量不足40 mm的敦煌市,设干旱和正常灌水两个处理,通过测定12份大豆品种的8个形态指标(株高、主茎节数、有效分枝数、单株荚数、单株粒数、单株粒重、单株生物量和百粒重)及小区产量,采用改进抗旱指数法及权重隶属函数值D对其进行抗旱性评价,并筛选出3份不同抗旱类型的大豆品种进行生理指标测定,验证该方法和指标。结果显示,与灌水处理(CK)相比较,干旱胁迫下的8个形态指标及产量,2014年除有效分枝数及百粒重差异不显著外,其余考察性状均达到了极显著差异,2015年所有考察表型性状均达到极显著差异;两年权重隶属函数法评价结果显著相关,小区产量与两年两种评价方法极显著相关;两种处理下,中度抗旱(中黄24)、弱抗旱品种(WDD00172)的SOD活性、POD活性、CAT活性、丙二醛含量、可溶性糖含量差异极显著,且与权重隶属函数值D显著相关,而强抗旱品种(晋豆21号)中SOD活性、POD活性、脯氨酸含量差异不显著,且6个生理指标与权重隶属函数值D均不相关。因此,权重隶属函数法和小区产量可作为大豆抗旱性评价单一可靠的方法与指标。 相似文献
6.
Luiz Henrique Saes Zobiole Robert John Kremer Rubem Silvério de Oliveira Jr Jamil Constantin 《Plant and Soil》2010,336(1-2):251-265
The crop area planted to conventional soybeans has decreased annually while that planted to glyphosate-resistant (RR) soybean has drastically increased mainly due to the wide adoption of glyphosate in current weed management systems. With the extensive use of glyphosate, many farmers have noted visual plant injury in RR soybean varieties after glyphosate application. A new generation designated as “second generation—RR2” has been recently developed and these RR2 cultivars already are commercially available for farmers and promoted as higher yielding relative to the previous RR cultivars. However, little information is currently available about the performance of RR2 soybean beyond commercial and farmer testimonial data. Thus, an evaluation of different glyphosate rates applied in different growth stages of the first and second generation of RR soybeans, revealed a significant decrease in photosynthesis. In general, increased glyphosate rate and late applications (V6) pronounced decrease photosynthetic parameters and consequently decreased in leaf area and shoot biomass production. In contrast, low rate and early applications were less damage for the RR soybean plants, suggesting that with early applications (V2), plants probably have more time to recover from glyphosate or its metabolites effects regarding late applications. 相似文献
7.
Producers in the southeastern USA face significant crop losses from the stink bugs Nezara viridula (L.), Euschistus servus (Say), and Chinavia hilaris (Say) (all Hemiptera: Pentatomidae). Cotton, peanut, and soybean are major agronomic crops and host plants of stink bugs in the region. We conducted a field plot study to measure the relative longevity of adult, unmated N. viridula males and females caged on peanut, cotton, and soybean to test three hypotheses: (1) differences in mortality are associated with differences in host plant food suitability, (2) mortality rates increase with age, and (3) males have higher mortality than females. Using survival analysis, we found that the sex of the individual did not affect survival rates on any of the three host plants. Survival was significantly higher in cotton and soybean than in peanut. Mortality rates increased with age in peanut, but not in soybean or cotton. The frequency of canopy temperatures above 35 °C was higher in peanut than in soybean. Peanut appears to be a less than ideal habitat in terms of canopy temperature and/or food quality for N. viridula adults. Both, cotton and soybean were equally suitable food resources for N. viridula adults prior to maturation of the plants. 相似文献
8.
The survivorship characteristics of two populations of Aedes caspius (Pallas) (Diptera: Culicidae) were compared in the laboratory. One population was sourced from Mourgues, where larvicides have been used continuously for approximately 40 years, and the other from Pont de Gau, where there has been no consistent mosquito control. The aims of the study were to ascertain the basic life history profiles of adults and to determine whether continuous larviciding affects inherent adult survivorship. Life tables were constructed to calculate the following life expectancy parameters: mean lifetime (tau(ad)); maximum lifetime (tau(max)), and daily survival rate (p(ad)). All three parameters were higher for females than for males (paired t-test, P < or = 0.001); male mean lifetime, maximum lifetime and daily survival rate were 4.95 +/- 0.94 days, 20.50 +/- 6.66 days and 0.79 +/- 0.05, respectively; female values were 14.74 +/- 3.68 days, 49.69 +/- 16.55 days and 0.93 +/- 0.02, respectively. No differences were found between the two populations, and no correlations were found between initial adult densities and their respective survival rates. The survivorship curves for Ae. caspius were type IV for males (mortality rates higher for young adults) and type III for females (mortality rates constant). 相似文献
9.
Influence of herbicide tolerant soybean production systems on insect pest populations and pest-induced crop damage 总被引:1,自引:0,他引:1
McPherson RM Johnson WC Mullinix BG Mills WA Peebles FS 《Journal of economic entomology》2003,96(3):690-698
Conventional soybean weed management and transgenic herbicide-tolerant management were examined to assess their effects on soybean insect pest populations in south Georgia in 1997 and 1998. Soybean variety had very little impact on the insect species observed, except that maturity group effects were observed for stink bug, primarily Nezara viridula (L.), population densities on some sampling dates. Stink bugs were more abundant on the early maturing varieties in mid-season. Velvetbean caterpillar, Anticarsia gemmatalis Hübner, potato leafhopper, Empoasca fabae (Harris), and grasshoppers Melanoplus spp. were more numerous on either conventional or herbicide-tolerant varieties on certain dates, although these differences were not consistent throughout the season. Soybean looper, Pseudoplusia includens (Walker), threecornered alfalfa hopper, Spissistilus festinus (Say), and whitefringed beetles, Graphognathus spp , demonstrated no varietal preference in this study. Few weed treatment differences were observed, but if present on certain sampling dates, then pest numbers were higher in plots where weeds were reduced (either postemergence herbicides or preplant herbicide plus postemergence herbicide). The exception to this weed treatment effect was grasshoppers, which were more numerous in weedy plots when differences were present. In post emergence herbicide plots, there were no differences in insect pest densities between the conventional herbicides (e.g., Classic, Select, Cobra, and Storm) compared with specific gene-inserted herbicide-tolerant materials (i.e., Roundup and Liberty). Defoliation, primarily by velvetbean caterpillar, was different between soybean varieties at some test sites but not different between herbicide treatments. We did not observe differences in seasonal abundance of arthropod pests between conventional and transgenic herbicide-tolerant soybean. 相似文献
10.
Nutritional assessment and fate of DNA of soybean meal from roundup ready or conventional soybeans using rats 总被引:2,自引:0,他引:2
This study was conducted to compare the safety of soybean meal prepared from genetically modified (GM) glyphosate-tolerant (Roundup Ready; RR) soybeans and conventional soybeans. Eighty Sprague-Dawley rats (40 males and 40 females) were randomly allotted to one of four groups according to sex and body weight for a 13-week feeding experiment. The rats were fed corn-based diets containing 60% conventional soybean meal, a mixture of 30% conventional and 30% RR soybean meal, 60% or 90% RR soybean meal. All diets were adjusted to an identical nutrient level except the 90% RR diet. The two soybean meals were similar in chemical analysis and amino acid composition. During the 13-week growth trial, body weight (P < 0.05) and feed intake (P < 0.05) decreased only in rats fed with 90% RR soybean meal at the first week. No treatment-related deaths occurred during the experiment. Gross necropsy findings, haematological or urinalysis values and clinical serum parameters showed no meaningful differences between rats fed the control and RR soybean meals. A 145 bp of cp4 epsps gene specific for the GM constructs from RR soybean meal or a 407 bp of lec gene from endogenous soybean DNA could not be detected in investigated masseter muscle samples. No adverse effects of glyphosate-tolerant soybean meal on rats were seen even at levels as high as 90% of the diet. 相似文献
11.
Jonathan G. Lundgren Louis S. Hesler Kelley Tilmon Kenton Dashiell Roy Scott 《Arthropod-Plant Interactions》2009,3(1):9-16
The direct effects of three soybean parentages, each represented by an Aphis glycines-resistant and susceptible isoline, on the fitness and performance of two key predators (Orius insidiosus and Harmonia axyridis) were evaluated in the laboratory. Predators were reared from hatch through adulthood in Petri dishes with cut trifoliolate
leaves of the designated soybean variety, using eggs of Ephestia kuehniella as surrogate prey to eliminate prey-mediated effects of the host plant. Preimaginal survival and development, sex ratio,
adult longevity, fecundity, and size were compared among treatments and a no-plant control. An additional experiment compared
life-history parameters of predators caged with soybean versus Ipomoea hederacea (ivyleaf morning glory). Aphid resistance reduced the adult longevity of H. axyridis, but O. insidiosus was unaffected by resistance traits. However, adult O. insidiosus lived longer on soybeans with Group C base genetics than the other soybean varieties. Other parameters were not affected
by soybean base genetics or resistance, but both predators generally performed worse on soybean than on I. hederacea or no-plant controls. The results suggest that soybean varietal selection, particularly with respect to A. glycines-resistance, may directly affect biological control agents. Also, implications of the generally poor suitability of soybean
for natural enemies are discussed within the context of current crop production practices.
Handling editor: Michael Smith 相似文献
12.
Thomas J. Mozdzer Curtis J. Hutto Paul A. Clarke Dorothy P. Field 《Restoration Ecology》2008,16(2):221-224
The cosmopolitan common reed ( Phragmites australis ) has been expanding into previously unoccupied wetland habitats throughout North America. This invasion by a non-native haplotype of Phragmites has become a major concern due to a reduction in plant diversity, reduction of faunal biodiversity, and changes in ecosystem structure. A randomized complete block design was used to compare the efficacy of two herbicides, glyphosate (Rodeo, Dow AgroSciences, IN, U.S.A.) and imazapyr (Habitat, BASF Corporation, NC, U.S.A.), on 1-ha Phragmites monoculture in a shallow borrow pit. Six foliar experimental treatments were applied consisting of (1) 2% glyphosate formulation, June application; (2) 2% glyphosate formulation, September application; (3) 2% imazapyr formulation, June application; (4) 2% imazapyr formulation, September application; (5) 5% imazapyr formulation, June application; and (6) 5% imazapyr, September application. Experimental plots were monitored yearly for two years after treatment. Relative importance values (RIV) were determined to assess the efficacy of herbicide treatments. We report that imazapyr foliar application is statistically superior to glyphosate in reducing Phragmites RIV, with no significant differences between the 2 and 5% formulations. Both herbicides are more effective in reducing Phragmites RIV if applied early in the growing season (June). No significant differences in non- Phragmites plant recolonization were observed between herbicide treatments over the two-year time course. These results suggest that imazapyr is superior in reducing Phragmites RIV, and that earlier applications of herbicides may be more effective on Phragmites . However, managers must note that adjacent nontarget plant species may be negatively affected by earlier treatments. 相似文献
13.
Carlos Alberto Moldes Leonardo Oliveira Medici Othon Silva Abrahão Siu Miu Tsai Ricardo Antunes Azevedo 《Acta Physiologiae Plantarum》2008,30(4):469-479
Glyphosate is a wide spectrum, non-selective, post-emergence herbicide. It acts on the shikimic acid pathway inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), thus obstructing the synthesis of tryptophan, phenylalanine, tyrosine and other
secondary products, leading to plant death. Transgenic glyphosate-resistant (GR) soybean [Glycine max (L.)] expressing an glyphosate-insensitive EPSPS enzyme has provided new opportunities for weed control in soybean production.
The effect of glyphosate application on chlorophyll level, lipid peroxidation, catalase (CAT), ascorbate peroxidase (APX),
guaiacol peroxidase (GOPX) and superoxide dismutase (SOD) activities, soluble amino acid levels and protein profile, in leaves
and roots, was examined in two conventional (non-GR) and two transgenic (GR) soybean. Glyphosate treatment had no significant
impact on lipid peroxidation, whilst the chlorophyll content decreased in only one non-GR cultivar. However, there was a significant
increase in the levels of soluble amino acid in roots and leaves, more so in non-GR than in GR soybean cultivars. Root CAT
activity increased in non-GR cultivars and was not altered in GR cultivars. In leaves, CAT activity was inhibited in one non-GR
and one GR cultivar. GOPX activity increased in one GR cultivar and in both non-GR cultivars. Root APX activity increased
in one GR cultivar. The soluble protein profiles as assessed by 1-D gel electrophoresis of selected non-GR and GR soybean
lines were unaffected by glyphosate treatment. Neither was formation of new isoenzymes of SOD and CAT observed when these
lines were treated by glyphosate. The slight oxidative stress generated by glyphosate has no relevance to plant mortality.
The potential antioxidant action of soluble amino acids may be responsible for the lack of lipid peroxidation observed. CAT
activity in the roots and soluble amino acids in the leaves can be used as indicators of glyphosate resistance. 相似文献
14.
Uptake and translocation of the elemental markers rubidium (Rb) and cesium (Cs) within adult soybean looper, Pseudoplusia includens (Walker), were determined using atomic absorption spectrophotometry in the laboratory in various feeding and mating treatments. Neonates were tested to determine marker transfer from male and female adults fed rubidium chloride (RbCl)-treated artificial nectar, cesium chloride (CsCI)-treated artificial nectar, or both. All females contained detectable levels of Rb, Cs, or both, which were obtained either through direct feeding or via spermatophores. Rubidium was present in females at significantly greater levels than Cs. No significant differences in Rb levels were observed between feeding or spermatophore acquisitions. Most neonates had significantly higher levels of Rb than Cs. In a field cage study to evaluate adult feeding and oviposition behavior on blooming cotton and blooming soybean treated with RbCl and CsCl, respectively, more eggs contained Rb than Cs, indicating greater feeding on cotton nectar than soybean nectar, regardless of the host plant upon which eggs were laid. Females laid more eggs on blooming soybean than on blooming cotton. Higher levels of Rb in cotton than Cs in soybean were recorded and may be attributed to initial elemental marker quantities available to the insects. This study provides the support for the generalized observations that soybean looper infestations in soybean can be related to feeding activities by adults in cotton. 相似文献
15.
Across a large area of the midwestern United States Corn Belt, the western corn rootworm beetle (Diabrotica virgifera virgifera LeConte, Coleoptera: Chrysomelidae) exhibits behavioral resistance to annual crop rotation. Resistant females exhibit increased locomotor activity and frequently lay eggs in soybean (Glycine max L.) fields, although they also lay eggs in fields of corn (Zea mays L.) and other locations. The goals of this study were (1) to determine whether there were any differences in ovipositional behavior and response to plant cues between individual rotation-resistant and wild-type females in the laboratory and (2) to examine the roles of, and interaction between, host volatiles, diet, and locomotor behavior as they related to oviposition. Because rootworm females lay eggs in the soil, we also examined the influence of host plant roots on behavior. In the first year of the study, rotation-resistant beetles were significantly more likely to lay eggs in the presence of soybean foliage and to feed on soybean leaf discs than wild-type females, but this difference was not observed in the second year. Oviposition by rotation-resistant females was increased in the presence of soybean roots, but soybean herbivory did not affect ovipositional choice. Conversely, ovipositional choice of wild-type females was not affected by the presence or identity of host plant roots encountered, and wild-type females consuming soybean foliage were more likely to lay eggs. 相似文献
16.
This study was conducted to compare the safety of soybean meal prepared from genetically modified (GM) glyphosate-tolerant (Roundup Ready; RR) soybeans and conventional soybeans. Eighty Sprague-Dawley rats (40 males and 40 females) were randomly allotted to one of four groups according to sex and body weight for a 13-week feeding experiment. The rats were fed corn-based diets containing 60% conventional soybean meal, a mixture of 30% conventional and 30% RR soybean meal, 60% or 90% RR soybean meal. All diets were adjusted to an identical nutrient level except the 90% RR diet. The two soybean meals were similar in chemical analysis and amino acid composition. During the 13-week growth trial, body weight (P?0.05) and feed intake (P?0.05) decreased only in rats fed with 90% RR soybean meal at the first week. No treatment-related deaths occurred during the experiment. Gross necropsy findings, haematological or urinalysis values and clinical serum parameters showed no meaningful differences between rats fed the control and RR soybean meals. A 145?bp of cp4 epsps gene specific for the GM constructs from RR soybean meal or a 407?bp of lec gene from endogenous soybean DNA could not be detected in investigated masseter muscle samples. No adverse effects of glyphosate-tolerant soybean meal on rats were seen even at levels as high as 90% of the diet. 相似文献
17.
Traction forces of male and female southern green stink bugs Nezara viridula (L.) were measured on adaxial leaves of green beans (Phaseolus vulgaris L. cv. ‘Nagauzura’), peas (Pisum sativum L. cv. ‘Hakuryu’), rice (Oryza sativa L. cv. ‘Mac hin sung’), and soybean (Glycine max [L.] Merr. cv. ‘Enrei’), as well as on glass as a control surface. Bugs attached well on three-dimensionally structured surfaces covered with anti-adhesive epicuticular wax crystals. Strongest pulls were generated on adaxial green bean leaves, corresponding to safety factors (traction force/body weight) of 11.1 and 11.6 in males and females, respectively. These values were slightly lower on soybean (males: 7.4, females: 8.0) and rice leaves (males: 8.9, females: 10.6). Trichomes and papillae are assumed to promote pentatomid bug’s attachment. On tabular, wax-covered pea leaves, safety factors decreased significantly to 1.7 and 1.6, in males and females, respectively. Differently, on non-structured glass, safety factors resembled those on rice and soybean leaves (males: 9.6, females: 8.0). No statistical differences in traction force and safety factor between sexes were detected on any substrate. Surface wettability did not significantly affect the results. Both robust claws and tough adhesive pads enable the N. viridula bugs to grasp and adhere to a wide range of various plant substrates, including such ones covered with anti-adhesive wax crystals. However, tabular, pruinose pea leaves were detected to prevent the foothold of the southern green stink bugs, although pea is known as one of their common host plants. 相似文献
18.
A field survey of plant and flower sex ratio and secondary sex characteristics was made in Silene alba. Female-biased plant sex ratios were found, as seems typical for the species. Sex ratio distribution correlated with a gradient of soil moisture (with the more moist area having a more female-biased ratio) and with changes in the density of Silene (intermediate and higher density areas having greater female bias). The floral sex ratio was significantly female-biased only at the site that was most female-biased in terms of plant sex ratio. Otherwise the population of flowers was significantly male-biased. Male and female plants harvested from the field differed in secondary sexual characteristics. Males had more flowers and invested proportionately more biomass in leaf, but less in root, stem and reproductive tissue than did females. Although both males and females were larger in terms of total dry weight at the moist site, males produced more flowers at the driest (high density) site. Here the female bias in plant sex ratio was intermediate, but the floral sex ratio was significantly male-biased. A glasshouse experiment was performed in which plants were grown at four densities. Density significantly influenced plant survivorship and the probability of flowering, and increased female bias in the pots, but it did not affect patterns of biomass allocation in flowering plants. Patterns of male and female biomass allocation did not differ in the experiment, except in terms of reproductive allocation (greater in females) and allocation to leaf, greater in males, but only at the lowest density. This work urges caution in interpreting differences between males and females in the field as secondary sex characteristics, since we find such properties to be overlapping under experimental conditions. It supports the idea that males and females of a species may sustain different reproductive output under differing conditions. 相似文献
19.
Both male and female solitary bees visit flowers for rewards. Sex related differences in foraging efficiency may also affect their probability to act as pollinators. In some major genera of solitary bees, males can be identified from a distance enabling a comparative foraging-behavior study. We have simultaneously examined nectar foraging of males and females of three bee species on five plant species in northern Israel. Males and females harvested equal nectar amounts but males spent less time in each flower increasing their foraging efficiency at this scale. The overall average visit frequencies of females and males was 27.2 and 21.6 visits per flower per minute respectively. Females flew shorter distances increasing their visit frequency, relative foraging efficiency and their probability to pollinate. The proportion of conspecific pollen was higher on females, indicating higher floral constancy and pollination probability. The longer flights of males increase their probability to cross-pollinate. Our results indicate that female solitary bees are more efficient foragers; females seem also to be more efficient pollinators but males contribute more to long-distance pollen flow. 相似文献
20.
Júlia Kuklinsky-Sobral Welington Luiz Araújo Rodrigo Mendes Aline Aparecida Pizzirani-Kleiner João Lúcio Azevedo 《Plant and Soil》2005,273(1-2):91-99
Endophytic bacteria are ubiquitous in most plant species influencing the host fitness by disease suppression, contaminant degradation, and plant growth promotion. This endophytic bacterial community may be affected by crop management such as the use of chemical compounds. For instance, application of glyphosate herbicide is common mainly due to the use of glyphosate-resistant transgenic plants. In this case, the bacterial equilibrium in plant–endophyte interaction could be shifted because some microbial groups are able to use glyphosate as a source of energy and nutrients, whereas this herbicide may be toxic to other groups. Therefore, the aim of this work was to study cultivable and noncultivable endophytic bacterial populations from soybean (Glycine max) plants cultivated in soil with and without glyphosate application (pre-planting). The cultivable endophytic bacterial community recovered from soybean leaves, stems, and roots included Acinetobacter calcoaceticus, A. junii, Burkholderiasp., B. gladioli, Enterobacter sakazaki, Klebsiella pneumoniae, Pseudomonas oryzihabitans, P. straminea, Ralstonia pickettii,and Sphingomonassp. The DGGE (Denaturing Gradient Gel Electrophoresis) analysis from soybean roots revealed some groups not observed by isolation that were exclusive for plants cultivated in soil with pre-planting glyphosate application, such as Herbaspirillum sp., and other groups in plants that were cultivated in soil without glyphosate, such as Xanthomonas sp. and Stenotrophomonas maltophilia. Furthermore, only two bacterial species were recovered from soybean plants by glyphosate enrichment isolation. They were Pseudomonas oryzihabitans and Burkholderia gladioliwhich showed different sensibility profiles to the glyphosate. These results suggest that the application at pre-planting of the glyphosate herbicide may interfere with the endophytic bacterial communitys equilibrium. This community is composed of different species with the capacity for plant growth promotion and biological control that may be affected. However, the evaluation of this treatment in plant production should be carried out by long-term experiments in field conditions. 相似文献