桑-蚕系统中镉的吸收、累积与迁移

对桑－蚕系统中镉的吸收,累积与迁移行为研究结果表明：（１）桑树对土壤镉污染有一定的耐性,桑树镉累积量和相对累积率与土壤镉浓度的关系可分别用回归方程Ｔａ＝ａ＋ｂｌｏｇ（Ｓｃ）和ｌｏｇ（Ｒａ）＝ａ＋ｂｌｏｇ（Ｓｃ）描述。（２）随着土壤镉浓度的增加,镉在桑树根部的分布率明显增加,地上部分的分布率有所降低,运转到叶片的比率明显降低。（３）蚕体,蚕砂和蚕茧的镉含量,镉累积量,随着桑叶镉含量的增加而增加。     

紫色土外源锌、镉形态的生物有效性

通过对7种形态Zn,Cd与有效态Zn,Cd(DTPA提取）的相关分析及各形态Zn,Cd与植株Zn,Cd含量的通径分析,评价各形态Zn,Cd的生物有效性及对植株的相对贡献和途径。结果表明,交换态Zn,Cd与有效态Zn,Cd呈极显著正相关（r=0.954,0.953),交换态Zn,Cd与植株Zn,Cd含量的通径系数为1．267、1．168,交换态Zn,Cd有效性高,具有最大的生物活性,对植株Zn,Cd含量的贡献最大。其它形态如碳酸盐结合态锌、氧化锰结合态Zn、Cd通过交换态作用于植株的通径链系数（间接通径系数）分别为0．856、0．592、0．723,上述形态对植株Zn,Cd含量也有影响,具有一定的生物相对有效性,但通过交换态间接作用。交换态不仅自身具有最大的生物有效性,而且还作为其它形态Zn,Cd生物有效性的中介,为紫色土其它Zn,Cd库流向植株的主要通道。     

湘北丘陵林-稻系统镉的迁移

为了研究中南丘陵区林-稻系统镉的迁移规律,2012年5月下旬至9月上旬(中稻稻季)在湘北汩罗市桃林林场选择两种类型的林-稻复合生态系统针对降水、地表径流、山塘水、稻田水及其中镉含量用收支平衡法进行了原位动态研究。结果表明,(1)以降水形式从区域外输入的镉是湘北地域地表系统主要的外来镉源,镉的输入主要集中在稻季的早期;(2)镉以雨水形式输入丘陵林-稻系统后,53%被林地截留,7.5%累积于山塘,11.6%存留于稻田,其余28.9%以稻田水和稻草、谷粒的形式输出林-稻系统;(3)不同林-稻系统比较,混-稻系统对外源镉的固持功能最强,松-稻系统其次,对照系统最弱,其原因在于各系统间岗地森林郁闭度存在差异,郁闭度大能增强岗地对镉的固持能力;(4)在岗地截留镉能力较弱的情况下,山塘能够减缓系统镉的迁移强度;(5)岗地输入山塘水中的大分子有机物能加强镉在稻田中累积。综上所述,林-稻系统镉的累积与雨水常年输入镉有关,系统内岗地森林郁闭度、与有机物输出相关的森林类型影响镉在系统中的迁移和分配。     

Cd accumulation in roots and shoots of durum wheat: the roles of transpiration rate and apoplastic bypass

Cadmium is readily taken up from soils by plants, depending on soil chemistry, and variably among species and cultivars; altered transpiration and xylem transport and/or translocation in the phloem could cause this variation in Cd accumulation, some degree of which is heritable. Using Triticum turgidum var. durum cvs Kyle and Arcola (high and low grain Cd accumulating, respectively), the objectives of this study were to determine if low-concentration Cd exposure alters transpiration, to relate transpiration to accumulation of Cd in roots and shoots at several life stages, and to evaluate the role of apoplastic bypass in the accumulation of Cd in shoots. The low abundance isotope (106)Cd was used to probe Cd translocation in plants which had been exposed to elemental Cd or were Cd-na?ve; apoplastic bypass was monitored using the fluorescent dye PTS (8-hydroxy-1,3,6-pyrenetrisulphonate). Differential accumulation of Cd by 'Kyle' and 'Arcola' could be partially attributed to the effect of Cd on transpiration, as exposure to low concentrations of Cd increased mass flow and concomitant Cd accumulation in 'Kyle'. Distinct from this, exposure of 'Arcola' to low concentrations of Cd reduced translocation of Cd from roots to shoots relative to root accumulation of Cd. It is possible, but not tested here, that sequestration mechanisms (such as phytochelatin production, as demonstrated by others) are the genetically controlled difference between these two cultivars that results in differential Cd accumulation. These results also suggest that apoplastic bypass was not a major pathway of Cd transport from the root to the shoot in these plants, and that most of the shoot Cd resulted from uptake into the stele of the root via the symplastic pathway.     

Absorption,distribution and binding of cadmium and zinc in irrigated rice plants

Summary Rice plants are grown in nutrient solutions (Hoagland-Arnon 1) containing different concentrations of cadmium (0.005; 0.010 and 0.100 ppm) and one concentration of zinc (0.070 ppm) traced respectively with¹⁰⁹Cd and⁶⁵Zn. Distribution and localization of both elements are studied at plant and cellular levels. Contrary to zinc, cadmium remains fixed by radicular sites and its translocation index is very low. Centrifugation of roots homogenates showed that % Cd recovered in the residue increases with Cd concentration and emphasize the high capacity of Cd fixation by walls when Cd external concentration is high. The relative distributions of Cd and Zn present at the same concentration in the growing medium are quite identical. In the 100,000×g supernatant fraction of roots, both elements are associated with compounds of low molecular weight, Cd being bound to higher MW ligands than zinc (1000 to 3000 for zinc). The fact that Cd was recovered in fractions in which most of³⁵S-cysteine was present suggests that in plants, like in animals, peptides rich in — SH residues may play a role in the fixation of heavy metals. Contribution no. B 101488 of the Biology-Radiation Protection and Medical Research Programme DG XII CEC.     

Cadmium in Chinese coals: Abundance,distribution, occurrence,and environmental effects

Cadmium (Cd) in coals has attracted much attention due to its potential toxicity. In the present study, we reviewed up-to-date information on the abundance, distribution, and modes of occurrence of Cd in Chinese coals, as well as its environmental effects. With the 3110 sets of data rearranged, the arithmetic and weighted mean of Cd concentration in Chinese coals were calculated. Using coal reserves in individual province and coal-forming ages as weighting factors, the estimated average Cd abundances in Chinese coals are 0.25 mg/kg and 0.42 mg/kg, respectively. Variations in Cd coal abundances in different geographical locations, coal-forming ages, and coal ranks are taken into account. The Cd abundances in northern Chinese coals are significantly lower than those in the southern Chinese coals. Sulfides, especially sphalerite, are the most common Cd carriers in coals. As a semi-volatile element, Cd can be partially transformed into a volatilized form during highly thermal process, which could enrich surrounding fly ash. The combination of electrostatic precipitator and flue gas desulphurization is proved to be a better effective method for reducing emissions of Cd. Heavy loadings of Cd in environmental compartments due to anthropogenic activities have posed a great challenge to atmosphere–land–ocean systems.     

Cadmium Accumulation by Jack-Bean and Sorghum in Hydroponic Culture

Among the technologies used to recuperate cadmium (Cd) contaminated soils, phytoextraction are particularly important, where the selection of suitable plants is critical to the success of the soil remediation. Thus, the objectives of this study were to evaluate the responses of jack-bean and sorghum to Cd supply and to quantify Cd accumulation by these species grown in hydroponic culture. The plants were subjected to 0, 15, 30, or 60 μmol Cd L^?1 in the nutrient solution, and gas exchange, plant growth and Cd accumulation were measured at 25 days after starting Cd treatments. The Cd supply severely reduced growth of shoots and roots in both species. In jack-bean, Cd decreased photosynthesis by 56–86%, stomatal conductance by 59–85% and transpiration by 48–80%. The concentrations and amounts of Cd accumulated in the plant tissues were proportional to the metal supply in the nutrient solution. Sorghum was more tolerant than jack-bean to Cd toxicity, but the latter showed a greater metal concentration and accumulation in the shoot. Therefore, jack-bean would be more suitable than sorghum for use in Cd phytoremediation programs based on phytoextraction.     

Cadmium transport, resistance, and toxicity in bacteria, algae, and fungi

Cadmium is an important environmental pollutant and a potent toxicant to bacteria, algae, and fungi. Mechanisms of Cd toxicity and resistance are variable, depending on the organism. It is very clear that the form of the metal and the environment it is studied in, play an important role in how Cd exerts its effect and how the organism(s) responds. A wide range of Cd concentrations have been used to designate resistance in organisms. To date, no concentration has been specified that is applicable to all species studied under standardized conditions. Cadmium exerts its toxic effect(s) over a wide range of concentrations. In most cases, algae and cyanobacteria are the most sensitive organisms, whereas bacteria and fungi appear to be more resistant. In some bacteria, plasmid-encoded resistance can lead to reduced Cd2+ uptake. However, some Gram-negative bacteria without plasmids are just as resistant to Cd as are bacteria containing plasmids encoding for Cd resistance. According to Silver and Misra (1984), there is no evidence for enzymatic or chemical transformations associated with Cd resistance. Insufficient information is available on the genetics of Cd uptake and resistance in cyanobacteria and algae. Mechanisms remain largely unknown at this point in time. Cadmium is toxic to these organisms, causing severe inhibition of such physiological processes as growth, photosynthesis, and nitrogen fixation at concentrations less than 2 ppm, and often in the ppb range (Tables 2 and 3). Cadmium also causes pronounced morphological aberrations in these organisms, which are probably related to deleterious effects on cell division. This may be direct or indirect, as a result of Cd effects on protein synthesis and cellular organelles such as mitochondria and chloroplasts. Cadmium is accumulated internally in algae (Table 4) as a result of a two-phase uptake process. The first phase involves a rapid physicochemical adsorption of Cd onto cell wall binding sites, which are probably proteins and (or) polysaccharides. This is followed by a lag period and then a slow, steady intracellular uptake. This latter phase is energy dependent and may involve transport systems used to accumulate other divalent cations, such as Mn2+ and Ca2+. Some data indicate that Cd resistance, and possibly uptake, in algae and cyanobacteria is controlled by a plasmid-encoded gene(s). Although considerable information is available on Cd toxicity to, and uptake in fungi, further work is clearly needed in several areas. There is little information about Cd uptake by filamentous fungi, and even in yeasts, information on the specificity, kinetics, and mechanisms of Cd uptake is limited.(ABSTRACT TRUNCATED AT 400 WORDS)     

Cadmium uptake in rat hepatocytes in relation to speciation and to complexation with metallothionein and albumin

Cadmium (Cd) uptake has been studied in primary cultures of rat hepatocytes focusing on the impact of inorganic and organic speciation. Uptake time-course studies over a 60-min exposure to 0.3 microM (109)Cd revealed a zero-time uptake and a slower process of accumulation which proceeds within minutes. (109)Cd uptake showed saturation kinetics (K(m) = 3.5 +/- 0.8 microM), and was highly sensitive to inhibition by Zn and Hg. There was no evidence for sensitivity to the external pH nor for any preferential transport of the free cation Cd(2+) over CdCl(n) (2-n) chloro-complexes. According to the assumption that only inorganic metal species are available, metal uptake decreased upon albumin (BSA) addition to the exposure media. In contrast, higher levels of (109)Cd accumulation were obtained under optimal conditions for Cd complexation by MT. Comparison among uptake data obtained under inorganic and organic conditions revealed that Cd-MT would be taken up 0.4 times as rapidly as Cd(inorg). We conclude that uptake of Cd in rat hepatocytes involves specific transport mechanism(s) subjected to Zn or Hg interactions. Uptake of inorganic Cd is not proportional to the levels of free Cd(2+) and does not involve the divalent cation transporter DCT1 nor the co-transporter Fe(2+)-H(+) NRAMP2. We found Cd-MT but not Cd-BSA to be available for the liver cells, and have estimated a binding affinity four orders of magnitude higher for Cd complexation with MT compared to BSA; MT may have a significant role in Cd delivery to the liver.     

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of four Hylotelephium spectabile populations exhibit differences in phytoextraction potential

Hylotelephium spectabile with high tolerance to cadmium (Cd) might be a potential candidate for phytoremediation. However, the mechanisms for Cd accumulation and tolerance in H. spectabile are poorly understood. Four H. spectabile populations, namely HB1, HB2, JS, and LN, were selected to investigate their Cd extraction potential and the underlying mechanism of Cd accumulation, focusing on subcellular distribution and antioxidant enzymes. The Cd concentration, bioconcentration factor and transfer factor of the LN was significantly higher than other populations, particularly with increasing Cd exposure, and no obvious growth inhibition observed. Segregation of excessive Cd to Cd-rich granule in LN was much higher than other populations which reveal one possible mechanism of Cd accumulation. A significant increase in superoxide dismutase (SOD) and catalase (CAT) activities with increasing Cd stress suggested SOD and CAT contribute to the Cd tolerance of H. spectabile. LN displayed significantly higher and constant peroxidase (POD) activities than other populations, which indicated that an effective mechanism existed in the LN to cope with Cd stress. Therefore, the subcellular distribution and antioxidant enzymes might play important roles in Cd accumulation and tolerance of H. spectabile. LN possessed high Cd extraction potential, and further studies under field conditions are warranted.     

Selenium and Cadmium Levels and Ratios in Prostates,Livers, and Kidneys of Nonsmokers and Smokers

Cadmium (Cd) from cigarette smoke, environmental, and nutritional sources accumulates in the human prostate where it interacts with selenium (Se) in a manner suggesting the formation of a 1:1 Cd–Se–protein complex. At low Cd exposures and adequate Se status, this interaction may be beneficial as it results in the detoxification of Cd. At higher exposures, Cd may weaken or abolish the anticarcinogenic effects of Se and increase prostate cancer risk. In some older men and especially in smokers, Cd levels in prostates reach levels in stoichiometric excess over Se, which may be the reason why heavy smokers are at higher risk of developing lethal forms of prostate cancer. In the liver and the kidneys, Cd accumulates as well, but in these organs, Cd is efficiently sequestered through metallothionein formation. In the prostate, this mode of detoxification is not available or less efficient, causing Cd to interact with Se and to increase prostate cancer risk.     

Physiological and biochemical analysis of mechanisms underlying cadmium tolerance and accumulation in turnip

The capacity of plants to accumulate cadmium (Cd) is significant for phytoremediation of Cd-polluted soils. Turnips cultivated in China include species featuring high Cd accumulation and some of these plants act as Cd hyperaccumulator landraces. These plants can accumulate over 100 mg Cd kg^-1 dry weight in leaves without injury. Hence, studies that explore mechanisms underlying Cd detoxification and transport in turnip plants are essential. In the present study, we compared physiological and biochemical changes in turnip leaves treated with two Cd concentrations to controls. We discovered that Cd stress significantly increased the enzymatic activities or compound contents in the antioxidant system, including members of the glutathione-ascorbic acid cycle, whereas oxidation of reactive oxygen species (ROS) remained stable. Cd treatments also increased the contents of phytochelatins as well as a number of amino acids. Based on these results, we conclude that turnips initiate a series of response processes to manage Cd treatment. First, the antioxidant system maintaining ROS homeostasis and osmotic adjustment is excited to maintain stability of cell osmotic potential. Cd is chelated into its stable form to reduce its toxicity. Cd is possibly transported to vacuoles or non-protoplasts for isolation. Amino acid synthesis may directly and indirectly play an important role in these processes. This study partly revealed physiological and biochemical mechanisms underlying turnip response to Cd stress and provides information on artificially increasing or decreasing Cd accumulation in turnips and other plants.     

Genetic basis of Cd tolerance and hyperaccumulation in Arabidopsis halleri

The genetic basis of Cd tolerance and hyperaccumulation was investigated in Arabidopsis halleri. The study was conducted in hydroponic culture with a backcross progeny, derived from a cross between A. halleri and a non-tolerant and non-accumulating related species Arabidopsis lyrata ssp. petraea, as well as with the parents of the backcross. The backcross progeny segregates for both cadmium (Cd) tolerance and accumulation. The results support that (i) Cd tolerance may be governed by more than one major gene, (ii) Cd tolerance and Cd accumulation are independent characters, (iii) Cd and Zn tolerances co-segregate suggesting that they are under pleiotropic genetic control, at least to a certain degree, (iv) the same result was obtained for Cd and Zn accumulation.     

Nanomolar concentrations of Cd2+ inhibit Ca2+ transport systems in plasma membranes and intracellular Ca2+ stores in intestinal epithelium

The interactions of Cd2+ with active Ca2+ transport systems in rat intestinal epithelial cells have been investigated. ATP-driven Ca2+ transport in basolateral plasma membrane vesicles was inhibited by Cd2+ with an I50 value of 1.6 nM free Cd2+ at 1 microM free Ca2+, using EGTA and HEEDTA to buffer Ca2+ and Cd2+ concentrations, respectively. The inhibition was competitive in nature since the Km value of Ca2+ increased with increasing Cd2+ concentrations while the Vmax remained constant. Cd2+ had similar effects on ATP-dependent Ca2+ uptake by permeabilized enterocytes, indicating that non-mitochondrial and mitochondrial Ca2+ stores are also inhibited by nanomolar concentrations of Cd2+. We conclude that ATP-driven Ca2+ transport systems are the most sensitive elements so far reported in Cd2+ intoxication.     

Cadmium distribution in maize inbred lines: Effects of pH and level of Cd supply

In order to investigate the physiological basis of the differential Cd distribution and the degree of variation of this Cd distribution among maize inbred lines, six inbreds designated earlier as ‘shoot Cd excluders’ (B73, H99, and H96) and ‘non-shoot Cd excluders’ (B37, H98, and N28) were grown in nutrient solution culture at different external Cd levels or at different pH. The characterization of the inbreds according to their shoot/root partitioning of Cd was consistent, independent of pH or level of Cd supply. The Cd concentrations in the plants were highest at the highest pH of the solution cultures. Generally, there was a positive correlation between the Cd concentrations in shoots and xylem exudates. It was shown that the Cd concentration in the roots is particularly important in the Cd distribution process. Above a ‘critical’ internal Cd concentration in the roots, specific for each inbred, the ability to retain Cd is strongly diminished. It is concluded that structural and/or physiological characteristics of the roots are involved in Cd partitioning.     

Root-selective expression of AtCAX4 and AtCAX2 results in reduced lamina cadmium in field-grown Nicotiana tabacum L.

To assess the impact of enhanced root vacuole cadmium (Cd) sequestration on leaf Cd accumulation under a low Cd dose, as generally occurs in agriculture, leaf Cd accumulation was examined in field-grown tobacco plants expressing genes encoding the high-capacity-Cd, tonoplast-localized, divalent cation/H antiporters AtCAX4 and AtCAX2 (AtCAX, Arabidopsis cation exchanger). It has been shown previously that root tonoplast vesicles isolated from plants expressing these genes, directed by root-selective promoters, show enhanced Cd transport activity, and young plants show enhanced root Cd accumulation when grown in solution culture containing 0.02 µ m Cd, a moderate Cd dose. In this article, we present results which show that the lower leaves of mature plants expressing AtCAX2 or AtCAX4 , under the control of two different root-selective promoters, accumulate 15%–25% less lamina Cd than control plants when grown in the field (3 years, three different collection methods). Reciprocal grafting experiments of AtCAX2 shoots onto control roots (and vice versa), grown in solution culture with 0.005 µ m Cd, indicated that the root controls Cd translocation and accumulation in the shoot in control and AtCAX2 and AtCAX4 tobacco plants exposed to low Cd concentration. The results are consistent with a model in which supplementation of Cd/H antiporter activity in root cell tonoplasts enhances root Cd sequestration, resulting in decreased translocation of Cd to the shoot of field-grown plants. These results suggest that human Cd intake from food and tobacco use could be reduced via the enhancement of root vacuolar sequestration of this pollutant.     

Role of the node in controlling traffic of cadmium, zinc, and manganese in rice

Heavy metals are transported to rice grains via the phloem. In rice nodes, the diffuse vascular bundles (DVBs), which enclose the enlarged elliptical vascular bundles (EVBs), are connected to the panicle and have a morphological feature that facilitates xylem-to-phloem transfer. To find a mechanism for restricting cadmium (Cd) transport into grains, the distribution of Cd, zinc (Zn), manganese (Mn), and sulphur (S) around the vascular bundles in node I (the node beneath the panicle) of Oryza sativa 'Koshihikari' were compared 1 week after heading. Elemental maps of Cd, Zn, Mn, and S in the vascular bundles of node I were obtained by synchrotron micro-X-ray fluorescence spectrometry and electron probe microanalysis. In addition, Cd K-edge microfocused X-ray absorption near-edge structure analyses were used to identify the elements co-ordinated with Cd. Both Cd and S were mainly distributed in the xylem of the EVB and in the parenchyma cell bridge (PCB) surrounding the EVB. Zn accumulated in the PCB, and Mn accumulated around the protoxylem of the EVB. Cd was co-ordinated mainly with S in the xylem of the EVB, but with both S and O in the phloem of the EVB and in the PCB. The EVB in the node retarded horizontal transport of Cd toward the DVB. By contrast, Zn was first stored in the PCB and then efficiently transferred toward the DVB. Our results provide evidence that transport of Cd, Zn, and Mn is differentially controlled in rice nodes, where vascular bundles are functionally interconnected.     

Physiological Responses and Tolerance Mechanisms to Cadmium in Conyza canadensis

Experiments were conducted to examine the effects of different concentrations of Cd on the performance of the Cd accumulator Conyza canadensis. Cd accumulation in roots and leaves (roots > leaves) increased with increasing Cd concentration in soil. High Cd concentration inhibited plant growth, increased the membrane permeability of leaves, and caused a significant decline in plant height and chlorophyll [chlorophyll (Chl) a, Chl b, and total Chl] content. Leaf ultrastructural analysis of spongy mesophyllic cells revealed that excessive Cd concentrations cause adverse effects on the chloroplast and mitochondrion ultrastructures of C. canadensis. However, the activities of antioxidant enzymes, such as superoxide dismutase, catalase, peroxidase, total non-protein SH compounds, glutathione, and phytochelatin (PC) concentrations, showed an overall increase. Specifically, the increase in enzyme activities demonstrated that the antioxidant system may play an important role in eliminating or alleviating the toxicity of Cd in C. canadensis. Furthermore, results demonstrate that PC synthesis in plant cells is related to Cd concentration and that PC production levels in plants are related to the toxic effects caused by soil Cd level. These findings demonstrate the roles played by these compounds in supporting Cd tolerance in C. canadensis.     

Cadmium-induced synthesis of metallothioneins in human lymphocytes and monocytes

Cd2+-binding proteins of peripheral blood lymphocytes and monocytes have not well been characterized so far, although they are expected to be a clue for understanding Cd2+ toxicity in those immune competent cells. We separated a family of Cd2+-binding proteins from Cd2+-exposed human peripheral blood lymphocytes by gel filtration chromatography, and characterized them by SDS-gel electrophoresis. The proteins showed electrophoretic behaviours closely similar to metallothioneins (MTs) of HeLa cells derived from human cervical carcinoma. The proteins were also found in Cd2+-exposed monocytes, and were inducible by Cd2+ in both lymphocytes and monocytes. Anti-MT serum specifically precipitated these proteins, which were thus identified as MTs. These results suggest that the two classes of the cells involved in the immune system possess a protective mechanism against Cd2+ through MTs. A variety of human lymphoid cell lines derived from both T and B cells were also found to have capacity to synthesize MTs in response to Cd2+.     

Parameter determination involved in phytotoxicity and transport of cadmium in rice seedlings

Hydroponic experiments were conducted with rice seedlings to determine parameters involved in phytotoxicity and transport of Cadmium (Cd). Results showed that a linear decrease in relative growth rate and water use efficiency was observed in rice seedlings with increasing Cd concentrations. More severe inhibitory effects on both parameters were found at the 4-d treatment. The effective concentrations (EC) were estimated using the Levenberg–Marquardt Algorithm. The EC values obtained from the relative growth rate were always smaller than these from water use efficiency, indicating that the former was more sensitive to change of Cd than the latter. Phyto-transport of Cd was apparent, but the distribution of Cd in different parts of rice seedlings was different and roots were the dominant sink for Cd accumulation (p < 0.05). Although the translocation of Cd into shoots was detectable, more Cd was detected in shoots at the 4-d treatments than that at the 2-d treatments (p < 0.05). The partial correlation analysis suggested that the total accumulation rate of Cd was highly related to the doses of Cd supplied (p < 0.05). In conclusion, Cd is problematic at relatively low concentrations for rice seedlings, and inhibitory effects are highly dependent on the selected response endpoints and the duration of exposure periods.