Genetic Improvement of Willow for Bioenergy and Biofuels

Willows (Salix spp.) are a very diverse group of catkin-bearing trees and shrubs that are widely distributed across temperate regions of the globe.Some species respond well to being grown in short rotation coppice (SRC) cycles,which are much shorter than conventional forestry.Coppicing reinvigorates growth and the biomass rapidly accumulated can be used as a source of renewable carbon for bioenergy and biofuels.As SRC willows re-distribute nutrients during the perennial cycle they require only minimal nitro...     

Short rotation coppice culture of willows and poplars as energy crops on metal contaminated agricultural soils

Phytoremediation, more precisely phytoextraction, has been placed forward as an environmental friendly remediation technique, that can gradually reduce increased soil metal concentrations, in particular the bioavailable fractions. The aim of this study was to investigate the possibilities of growing willows and poplars under short rotation coppice (SRC) on an acid, poor, sandy metal contaminated soil, to combine in this way soil remediation by phytoextraction on one hand, and production of biomass for energy purposes on the other. Above ground biomass productivities were low for poplars to moderate for willows, which was not surprising, taking into account the soil conditions that are not very favorable for growth of these trees. Calculated phytoextraction efficiency was much longer for poplars than these for willows. We calculated that for phytoextraction in this particular case it would take at least 36 years to reach the legal threshold values for cadmium, but in combination with production of feedstock for bioenergy processes, this type of land use can offer an alternative income for local farmers. Based on the data of the first growing cycle, for this particular case, SRC of willows should be recommended.     

QTL Mapping of Enzymatic Saccharification in Short Rotation Coppice Willow and Its Independence from Biomass Yield

Short rotation coppice (SRC) willows (Salix spp.) are fast-growing woody plants which can achieve high biomass yields over short growth cycles with low agrochemical inputs. Biomass from SRC willow is already used for heat and power, but its potential as a source of lignocellulose for liquid transport biofuels has still to be assessed. In bioethanol production from lignocellulose, enzymatic saccharification is used as an approach to release glucose from cellulose in the plant cell walls. In this study, 138 genotypes of a willow mapping population were used to examine variation in enzymatic glucose release from stem biomass to study relationships between this trait and biomass yield traits and to identify quantitative trait loci (QTL) associated with enzymatic saccharification yield. Significant natural variation was found in glucose yields from willow stem biomass. This trait was independent of biomass yield traits. Four enzyme-derived glucose QTL were mapped onto chromosomes V, X, XI, and XVI, indicating that enzymatic saccharification yields are under significant genetic influence. Our results show that SRC willow has strong potential as a source of bioethanol and that there may be opportunities to improve the breeding programs for willows for increasing enzymatic saccharification yields and biofuel production.     

Dedicated biomass crops can enhance biodiversity in the arable landscape

Suggestions that novel, non‐food, dedicated biomass crops used to produce bioenergy may provide opportunities to diversify and reinstate biodiversity in intensively managed farmland have not yet been fully tested at the landscape scale. Using two of the largest, currently available landscape‐scale biodiversity data sets from arable and biomass bioenergy crops, we take a taxonomic and functional trait approach to quantify and contrast the consequences for biodiversity indicators of adopting dedicated biomass crops on land previously cultivated under annual, rotational arable cropping. The abundance and community compositions of biodiversity indicators in fields of break and cereal crops changed when planted with the dedicated biomass crops, miscanthus and short rotation coppiced (SRC) willow. Weed biomass was consistently greater in the two dedicated biomass crops than in cereals, and invertebrate abundance was similarly consistently higher than in break crops. Using canonical variates analysis, we identified distinct plant and invertebrate taxa and trait‐based communities in miscanthus and SRC willows, whereas break and cereal crops tended to form a single, composite community. Seedbanks were shown to reflect the longer term effects of crop management. Our study suggests that miscanthus and SRC willows, and the management associated with perennial cropping, would support significant amounts of biodiversity when compared with annual arable crops. We recommend the strategic planting of these perennial, dedicated biomass crops in arable farmland to increase landscape heterogeneity and enhance ecosystem function, and simultaneously work towards striking a balance between energy and food security.     

Meeting Sustainability Requirements for SRC Bioenergy: Usefulness of Existing Tools,Responsibilities of Involved Stakeholders,and Recommendations for Further Developments

Short rotation coppice (SRC) is considered an important biomass supply option for meeting the European renewable energy targets. This paper presents an overview of existing and prospective sustainability requirements, Member State reporting obligations and parts of the methodology for calculating GHG emissions savings within the EU Renewable Energy Directive (RED), and shows how these RED-associated sustainability criteria may affect different stakeholders along SRC bioenergy supply chains. Existing and prospective tools are assessed on their usefulness in ensuring that SRC bioenergy is produced with sufficient consideration given to the RED-associated criteria. A sustainability framework is outlined that aims at (1) facilitating the development of SRC production systems that are attractive from the perspectives of all stakeholders, and (2) ensuring that the SRC production is RED eligible. Producer manuals, EIAs, and voluntary certification schemes can all be useful for ensuring RED eligibility. However, they are currently not sufficiently comprehensive, neither individually nor combined, and suggestions for how they can be more complementary are given. Geographical information systems offer opportunities for administrative authorities to provide stakeholders with maps or databases over areas/fields suitable for RED-eligible SRC cultivation. However, proper consideration of all relevant aspects requires that all stakeholders in the SRC supply chain become engaged in the development of SRC production systems and that a landscape perspective is used.     

Effects of nitrogen fertilization in shrub willow short rotation coppice production – a quantitative review

Sustained interest in producing renewable energy from dedicated woody biomass crops, such as shrub willow (Salix spp.), through short rotation coppice (SRC) has resulted in a substantial amount of published research on SRC over the past few decades. One area of constant focus has been the nutritional requirements for optimal growth and yield. Inconsistency in the results of individual research trials has likely been a driver of repeated experimentation. This review is intended to provide a quantitative examination of the effect of fertilization treatments on willow biomass yield in field conditions. Data from the literature were collected and summarized to test for significant sources of variation in willow biomass nitrogen (N) pools of common SRC genotypes used in North American and European research programs. A meta‐analysis was conducted on studies comparing synthetic or organic sources of N willow fertilization to an unfertilized control treatment to test for yield response. Overall, the majority of responses to fertilization were positive, although variation by species, N source material, and crop age were found. While no clear pattern in N dosage response was observed, the level of yield response was correlated with geographic and climatic variables. Nitrogen export levels were fairly predictable, and the synthesis presented here can be used to refine current guidelines. Environmental and economic aspects are also considered.     

Effects of Cutting Phenology (Non-dormant Versus Dormant) on Early Growth Performance of Three Willow Clones Grown Under Different Weed Treatments and Planting Dates

To assess the effects of cutting phenology on early growth performance of three willow clones grown under different weed treatments and planting dates, freshly harvested (non-dormant) and cold-stored (dormant) cuttings from willow clone Tora, Jorr, and Olof were planted in bucket experiment outdoors in central Sweden on five planting dates (May–June 2013) with or without a model weed (spring barley). Non-dormant cuttings sprouted faster than dormant cuttings when planted early in the season. For cuttings planted later in the season, bud sprouting was affected only by willow clone. Aboveground biomass production was affected by cutting phenology, planting date, clone, and weed treatment. When planted on May 3 and May 10, biomass produced from non-dormant and dormant cuttings did not differ, while willows grown from dormant cuttings produced 59% more aboveground biomass than willows grown from non-dormant cuttings when planted on May 24–June 16. Tora produced on average 12% more biomass than Jorr and Olof, and weed competition reduced aboveground biomass production on average with 36%. The ability of willow to suppress weeds (WSA) was 26 (non-dormant cuttings) and 12% (dormant cuttings) higher for willows planted on May 3 compared with WSA of willows grown from cuttings planted later in the season. The ability to tolerate competition from weeds (WT) was 51 and 52% lower for willows grown from non-dormant and dormant cuttings planted late in the season compared with WT of willows planted earlier in the season. We conclude that planting with long-term cold storage of willow cuttings can be replaced with planting freshly harvested cuttings when planting is performed in early season, and that weed competition strongly reduces biomass production. Weed control during the establishment phase is crucial in order to maximize willow biomass production.     

Selection, breeding and engineering of microalgae for bioenergy and biofuel production

Microalgal production technologies are seen as increasingly attractive for bioenergy production to improve fuel security and reduce CO(2) emissions. Photosynthetically derived fuels are a renewable, potentially carbon-neutral and scalable alternative reserve. Microalgae have particular promise because they can be produced on non-arable land and utilize saline and wastewater streams. Furthermore, emerging microalgal technologies can be used to produce a range of products such as biofuels, protein-rich animal feeds, chemical feedstocks (e.g. bioplastic precursors) and higher-value products. This review focuses on the selection, breeding and engineering of microalgae for improved biomass and biofuel conversion efficiencies.     

ASSOCIATED BACTERIA INCREASE THE PHYTOEXTRACTION OF CADMIUM AND ZINC FROM A METAL-CONTAMINATED SOIL BY MYCORRHIZAL WILLOWS

In order to enhance phytoremediation efficiency, we investigated the effects of dual inoculation with ectomycorrhizal fungi and the ectomycorrhiza associated bacteria Micrococcus luteus and Sphingomonas sp. on the growth and metal accumulation of willows (Salix viminalis x caprea) on contaminated soil. The bacterial strains were previously collected from sporocarps of ectomycorrhizal fungi. The bacteria increased plant growth and the mycorrhizal dependency of willows colonized with the ectomycorrhizal fungus Hebeloma crustuliniforme. The total cadmium (Cd) and zinc (Zn) accumulation in the shoot biomass was increased after inoculation with the fungal strain Hebeloma crustuliniforme in combination with Micrococcus luteus up to 53% and in combination with Sphingomonas sp. up to 62%, respectively. The dual inoculation in combination with Laccaria laccata did not increase the accumulation of Cd and Zn in the willows. We conclude that associated bacteria can enhance the ectomyorrhiza formation and growth of willows and, thereby, the Cd and Zn accumulation in the plant biomass. The results suggest that bacterial support of root growth promoting ectomycorrhizal fungi may be a promising approach to improve the remediation of metal-contaminated soils by using willows.     

Land availability and potential biomass production with poplar and willow short rotation coppices in Germany

Several factors influence land availability for the growth of short rotation coppices (SRC) with fast‐growing tree species, including the nationwide availability of agricultural land, economic efficiency, ecological impacts, political boundaries and environmental protection regulations. In this study, we analysed the growing potential of poplar and willow SRC for bioenergy purposes in Germany without negative ecological impacts or land use conflicts. The potential biomass production using SRC on agricultural land in Germany was assessed taking into account ecological, ethical, political and technical restrictions. Using a geographic information system (GIS), digital site maps, climate data and a digital terrain model, the SRC biomass production potential on cropland and grassland was estimated using water supply and mean temperature during the growing season as parameters. From this analysis, a yield model for SRC was developed based on the analysed growth data and site information of 62 short rotation plantations in Germany and France. To assess the technical, ethical and ecological potential of SRC, restrictions in protected areas, technical constraints and competition with food and feed production were investigated. Our results revealed that approximately 18% (2.12 Mio. ha) of cropland and 54% (2.5 Mio. ha) of grassland in Germany were highly suitable for SRC plantations, providing favourable water supplies and mean temperatures during the growing season. These identified sites produced an average yield of more than 14 tons of dry matter per hectare per year. Due to local climate and soil conditions, the federal states in northern and eastern Germany had the highest theoretical SRC potential for agricultural land. After considering all ecological, ethical, political and technical restrictions, as well as future climate predictions, 5.7% (680 000 ha) of cropland and 33% (1.5 Mio. ha) of grassland in Germany were classified as suitable for biomass production with fast‐growing tree species in SRC.     

Characterisation of genetic diversity in potential biomass willows (Salix spp.) by RAPD and AFLP analyses.

The genus Salix (willow) contains a number of species which have great potential value as biomass crops in short rotation coppice (SRC). Efforts to improve biomass willows by breeding are currently hampered by the limited information available on genetic diversity and on genetic relationships within and among species, clones, and hybrids in the gene pool. Hybridisation occurs commonly in nature and the relatedness of many clones is unclear. Molecular markers were used to assess genetic diversity in a reference set of willows maintained within the U.K. National Collection and 16 elite clones currently being evaluated in field trials at several European sites. The two marker systems tested, RAPDs and AFLPs, were equally informative for revealing relationships within the reference set of clones. No differences were observed when alternative similarity coefficients were compared or when analysis was restricted to the use of polymorphic bands only. Good agreement with available knowledge of the clonal origins was obtained and one instance of duplicate clones was identified. AFLPs revealed more genetic diversity and discriminated between closely related clones. A difference in the relationships revealed was observed with one AFLP primer combination. RAPDs were more problematic, both in terms of reproducibility and scorability.     

Perennial biomass cropping and use: Shaping the policy ecosystem in European countries

Demand for sustainably produced biomass is expected to increase with the need to provide renewable commodities, improve resource security and reduce greenhouse gas emissions in line with COP26 commitments. Studies have demonstrated additional environmental benefits of using perennial biomass crops (PBCs), when produced appropriately, as a feedstock for the growing bioeconomy, including utilisation for bioenergy (with or without carbon capture and storage). PBCs can potentially contribute to Common Agricultural Policy (CAP) (2023–27) objectives provided they are carefully integrated into farming systems and landscapes. Despite significant research and development (R&D) investment over decades in herbaceous and coppiced woody PBCs, deployment has largely stagnated due to social, economic and policy uncertainties. This paper identifies the challenges in creating policies that are acceptable to all actors. Development will need to be informed by measurement, reporting and verification (MRV) of greenhouse gas emissions reductions and other environmental, economic and social metrics. It discusses interlinked issues that must be considered in the expansion of PBC production: (i) available land; (ii) yield potential; (iii) integration into farming systems; (iv) R&D requirements; (v) utilisation options; and (vi) market systems and the socio-economic environment. It makes policy recommendations that would enable greater PBC deployment: (1) incentivise farmers and land managers through specific policy measures, including carbon pricing, to allocate their less productive and less profitable land for uses which deliver demonstrable greenhouse gas reductions; (2) enable greenhouse gas mitigation markets to develop and offer secure contracts for commercial developers of verifiable low-carbon bioenergy and bioproducts; (3) support innovation in biomass utilisation value chains; and (4) continue long-term, strategic R&D and education for positive environmental, economic and social sustainability impacts.     

Quantifying the climate change effects of bioenergy systems: Comparison of 15 impact assessment methods

Ongoing concern over climate change has led to interest in replacing fossil energy with bioenergy. There are different approaches to quantitatively estimate the climate change effects of bioenergy systems. In the present work, we have focused on a range of published impact assessment methods that vary due to conceptual differences in the treatment of biogenic carbon fluxes, the type of climate change impacts they address and differences in time horizon and time preference. Specifically, this paper reviews fifteen different methods and applies these to three hypothetical bioenergy case studies: (a) woody biomass grown on previously forested land; (b) woody biomass grown on previous pasture land; and (b) annual energy crop grown on previously cropped land. Our analysis shows that the choice of method can have an important influence on the quantification of climate change effects of bioenergy, particularly when a mature forest is converted to bioenergy use as it involves a substantial reduction in biomass carbon stocks. Results are more uniform in other case studies. In general, results are more sensitive to specific impact assessment methods when they involve both emissions and removals at different points in time, such as for forest bioenergy, but have a much smaller influence on agricultural bioenergy systems grown on land previously used for pasture or annual cropping. The development of effective policies for climate change mitigation through renewable energy use requires consistent and accurate approaches to identification of bioenergy systems that can result in climate change mitigation. The use of different methods for the same purpose: estimating the climate change effects of bioenergy systems, can lead to confusing and contradictory conclusions. A full interpretation of the results generated with different methods must be based on an understanding that the different methods focus on different aspects of climate change and represent different time preferences.     

Short-Rotation Coppice of Willow for Phytoremediation of a Metal-Contaminated Agricultural Area: A Sustainability Assessment

Large areas of land contaminated with cadmium (Cd), lead (Pb), and zinc (Zn) are currently in agricultural production in the Campine region in Belgium. Cadmium contents in food and fodder crops frequently exceed legal threshold values, resulting in crop confiscation. This imposes a burden on agriculture and regional policy and, therefore, encourages proper soil management. One way to increase agricultural income and improve soil quality is by growing alternative nonfood crops such as willows in short-rotation coppice (SRC) systems that remediate the soil. This paper compares SRC of willow with rapeseed and energy maize regarding four attributes: metal accumulation capacity, gross agricultural income per hectare, CO₂ emission avoidance potential, and agricultural acceptance. Based on multicriteria decision analysis, we conclude that, although SRC of willow has a high potential as an energy and remediating crop, it is unlikely to be implemented on the short term in Flanders unless the economic incentives for the farmers are improved.     

Renewable Energy from Willow Biomass Crops: Life Cycle Energy,Environmental and Economic Performance

Short-rotation woody crops (SRWC) along with other woody biomass feedstocks will play a significant role in a more secure and sustainable energy future for the United States and around the world. In temperate regions, shrub willows are being developed as a SRWC because of their potential for high biomass production in short time periods, ease of vegetative propagation, broad genetic base, and ability to resprout after multiple harvests. Understanding and working with willow's biology is important for the agricultural and economic success of the system.

The energy, environmental, and economic performance of willow biomass production and conversion to electricity is evaluated using life cycle modeling methods. The net energy ratio (electricity generated/life cycle fossil fuel consumed) for willow ranges from 10 to 13 for direct firing and gasification processes. Reductions of 70 to 98 percent (compared to U.S. grid generated electricity) in greenhouse gas emissions as well as NO_x, SO₂, and particulate emissions are achieved.

Despite willow's multiple environmental and rural development benefits, its high cost of production has limited deployment. Costs will be lowered by significant improvements in yields and production efficiency and by valuing the system's environmental and rural development benefits. Policies like the Conservation Reserve Program (CRP), federal biomass tax credits and renewable portfolio standards will make willow cost competitive in the near term.

The avoided air pollution from the substitution of willow for conventional fossil fuel generated electricity has an estimated damage cost of $0.02 to $0.06 kWh^?1. The land intensity of about 4.9 × 10^?5 ha-yr/kWh is greater than other renewable energy sources. This may be considered the most significant limitation of willow, but unlike other biomass crops such as corn it can be cultivated on the millions of hectares of marginal agricultural lands, improving site conditions, soil quality and landscape diversity. A clear advantage of willow biomass compared to other renewables is that it is a stock resource whereas wind and PV are intermittent. With only 6 percent of the current U.S. energy consumption met by renewable sources the accelerated development of willow biomass and other renewable energy sources is critical to address concerns of energy security and environmental impacts associated with fossil fuels.     

Integration of microalgae cultivation with industrial waste remediation for biofuel and bioenergy production: opportunities and limitations

There is currently a renewed interest in developing microalgae as a source of renewable energy and fuel. Microalgae hold great potential as a source of biomass for the production of energy and fungible liquid transportation fuels. However, the technologies required for large-scale cultivation, processing, and conversion of microalgal biomass to energy products are underdeveloped. Microalgae offer several advantages over traditional 'first-generation' biofuels crops like corn: these include superior biomass productivity, the ability to grow on poor-quality land unsuitable for agriculture, and the potential for sustainable growth by extracting macro- and micronutrients from wastewater and industrial flue-stack emissions. Integrating microalgal cultivation with municipal wastewater treatment and industrial CO(2) emissions from coal-fired power plants is a potential strategy to produce large quantities of biomass, and represents an opportunity to develop, test, and optimize the necessary technologies to make microalgal biofuels more cost-effective and efficient. However, many constraints on the eventual deployment of this technology must be taken into consideration and mitigating strategies developed before large scale microalgal cultivation can become a reality. As a strategy for CO(2) biomitigation from industrial point source emitters, microalgal cultivation can be limited by the availability of land, light, and other nutrients like N and P. Effective removal of N and P from municipal wastewater is limited by the processing capacity of available microalgal cultivation systems. Strategies to mitigate against the constraints are discussed.     

Correspondence of ectomycorrhizal diversity and colonisation of willows (Salix spp.) grown in short rotation coppice on arable sites and adjacent natural stands

Willows (Salix spp.) are mycorrhizal tree species sometimes cultivated as short rotation coppice (SRC) on arable sites for energy purposes; they are also among the earliest plants colonising primary successional sites in natural stands. The objective of this study was to analyse the degree of colonisation and diversity of ectomycorrhizal (EM) communities on willows grown as SRC in arable soils and their adjacent natural or naturalized stands. Arable sites usually lack ectomycorrhizal host plants before the establishment of SRC, and adjacent natural or naturalized willow stands were hypothesized to be a leading source of ectomycorrhizal inoculum for the SRC. Three test sites including SRC stands (Salix viminalis, Salix dasyclados, and Salix schwerinii) and adjacent natural or naturalized (Salix caprea, Salix fragilis, and Salix?×?mollissima) stands in central Sweden were investigated on EM colonisation and morphotypes, and the fungal partners of 36 of the total 49 EM fungi morphotypes were identified using molecular tools. The frequency of mycorrhizas in the natural/naturalized stands was higher (two sites) or lower (one site) than in the corresponding cultivated stands. Correspondence analysis revealed that some EM taxa (e.g. Agaricales) were mostly associated with cultivated willows, while others (e.g. Thelephorales) were mostly found in natural/naturalized stands. In conclusion, we found strong effects of sites and willow genotype on EM fungi formation, but poor correspondence between the EM fungi abundance and diversity in SRC and their adjacent natural/naturalized stands. The underlying mechanism might be selective promotion of some EM fungi species by more effective spore dispersal.     

Wasserlinsen als Nutzpflanzen

Duckweeds as crop plants Members of the plant family Lemnaceae (duckweeds) are not only interesting because they represent the smallest flowering plants; they possess also the fastest rates of producing biomass. As aquatic plants, duckweed production is not in competition with other agricultural crops that require fertile land while the cultivation of duckweeds does not contribute to further eutrophication of surface water. Instead, they can be cultivated on municipal or agricultural waste water and remove the nutrients during their propagation and growth. Duckweeds can thus be used for cleaning of waste water and the resulting biomass can be valuable starting material for animal feeds and the production of biofuels. Research focusing on these goals has begun to transfer from research laboratories to pilot plants in different parts of the world, e.g. in New Jersey and North Carolina, USA; Chengdu, P. R. China; and Armidale, Australia.     

Demand for biomass to meet renewable energy targets in the United States: implications for land use

Renewable energy policies in the electricity and transportation sectors in the United States are expected to create demand for biomass and food crops (corn) that could divert land from food crop production. We develop a dynamic, open‐economy, price‐endogenous multi‐market model of the US agricultural, electricity and transportation sectors to endogenously determine the quantity and mix of bioenergy likely to be required to meet the state Renewable Portfolio Standards (RPSs) and the federal Renewable Fuel Standard (RFS) if implemented independently or jointly (RFS & RPS) over the 2007–2030 period and their implications for the extent and spatial pattern of diversion of land from other uses for biomass feedstock production. We find that the demand for biomass ranges from 100 million metric tons (MMT) under the RPS alone to 310 MMT under the RFS & RPS; 70% of the biomass in the latter case can be met by crop and forest residues, while the rest can be met by devoting 3% of cropland to energy crop production with 80% of this being marginal land. Our findings show significant potential to meet current renewable energy goals by expanding high‐yielding energy crop production on marginal land and using residues without conflicting with food crop production.     

High value of short rotation coppice plantations for phytodiversity in rural landscapes

The demand for wood from short rotation coppice (SRC) plantations as a renewable energy source is currently increasing and could affect biodiversity in agricultural areas. The objective was to evaluate the contribution of SRC plantations to phytodiversity in agricultural landscapes assessed as species richness, species–area relationships, Shannon indices, detrended correspondence analysis on species composition, Sørensen similarities, habitat preference proportions, and species proportions found in only one land use. Vegetation surveys were conducted on 12 willow (Salix spp.) and three poplar (Populus spp.) coppice sites as well as on surrounding arable lands, grasslands and forests in central Sweden and northern Germany. SRC plantations were richer in plant species (mean: 30 species per 100 m²) than arable land (10), coniferous forests (13) and mixed forests in Germany (12). Comparing SRC plantations with other land uses, we found lowest similarities in species composition with arable lands, coniferous forests and German mixed forests and highest similarities with marginal grassland strips, grasslands and Swedish mixed forests. Similarity depended on the SRC tree cover: at increased tree cover, SRC plantations became less similar to grasslands but more similar to forests. The SRC plantations were composed of a mixture of grassland (33%), ruderal (24%) and woodland (15%) species. Species abundance in SRC plantations was more heterogeneous than in arable lands. We conclude that SRC plantations form novel habitats leading to different plant species composition compared to conventional land uses. Their landscape‐scale value for phytodiversity changes depending on harvest cycles and over time. As a structural landscape element, SRC plantations contribute positively to phytodiversity in rural areas, especially in land use mosaics where these plantations are admixed to other land uses with dissimilar plant species composition such as arable land, coniferous forest and, at the German sites, also mixed forest.