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1.
Maize (Zea mays L.) Dwarf8-1 (D8-1) is an andromonoecious dwarf mutant proposed to be involved in gibberellin (GA) reception (Fujioka et al. 1988b; Harberd and Freeling 1989). The mutant D8-1 is dominant and GA-nonresponsive (Phinney 1956). We show by map position and similarity of phenotype that five additional dwarf mutants are D8 alleles. We show by map position and similarity of phenotype that a second andromonoecious dwarf mutant, D9-1, defines a duplicate gene. Maize D9-1 and each dominant D8 allele specify a different plant stature, from very mild to very severe dwarfism. Plants of D9-1 and all dominant D8 alleles, except D8-1591, were GA-nonresponsive when treated with 7500 nmol GA3. The behavior of the mild dwarf D8-1591 was unique in that a small but significant growth response was detected (37% for D8-1591 vs. 130% for the wild type) when treated with 7500 nmol GA3. These results establish that all dwarf genotypes, except D8-1591, in one dose set a maximum limit on plant growth and block the normal response to GA. When treated with the GA-synthesis inhibitor paclobutrazol, plants of all dwarf genotypes and wild-type siblings were severely dwarfed. Plants of all dwarf genotypes treated with the GA-synthesis inhibitor paclobutrazol and GA3 were returned to their normal dwarf phenotype. Dominant dwarfing, delayed flowering, increased tillering, and anther development in the ear are characteristic features of D9-1 and all D8 alleles. The GA-synthesis-deficient dwarfs also have these characteristic features. We discuss the function of the wild-type gene product in the context of the observed results.Abbreviations D8 Dwarf8 - D9 Dwarf9 - GA(n) gibberellin A(n) - GA3 gibberellic acid - MNL Maize Genetics Cooperation Newsletter - NIL near-isogenic lines - RFLP restriction fragment length polymorphism - WT wild type This work was supported, in part, by a National Science Foundation Plant Postdoctoral Fellowship to R.G.W., by grants from NIH and ICI Seeds to M.F., the NSF Center for Plant Developmental Biology and the California Agriculture Experiment Station. Much of the work was done in the laboratory of Tim Helentjaris and was supported by a grant from Pioneer Hi-Bred Int'l. The generous gifts of the dominant dwarfing mutants from M.G. Neuffer and O.E. Nelson Jr. are gratefully acknowledged.  相似文献   

2.
Mutations in the biosynthesis or signaling pathways of gibberellin (GA) can cause dwarfing phenotypes in plants, and the use of such mutations in plant breeding was a major factor in the success of the Green Revolution. DELLA proteins are GA signaling repressors whose functions are conserved in different plant species. Recent studies show that GA promotes stem growth by causing degradation of DELLA proteins via the ubiquitin-proteasome pathway. The most widely utilized dwarfing alleles in wheat (Triticum aestivum; e.g. Rht-B1b and Rht-D1b) encode GA-resistant forms of a DELLA protein that function as dominant and constitutively active repressors of stem growth. All of the previously identified dominant DELLA repressors from several plant species contain N-terminal mutations. Here we report on a novel dwarf mutant from Brassica rapa (Brrga1-d) that is caused by substitution of a conserved amino acid in the C-terminal domain of a DELLA protein. Brrga1-d, like N-terminal DELLA mutants, retains its repressor function and accumulates to high levels, even in the presence of GA. However, unlike wild-type and N-terminal DELLA mutants, Brrga1-d does not interact with a protein component required for degradation, suggesting that the mutated amino acid causes dwarfism by preventing an interaction needed for its degradation. This novel mutation confers nondeleterious dwarf phenotypes when transferred to Arabidopsis (Arabidopsis thaliana) and oilseed rape (Brassica napus), indicating its potential usefulness in other crop species.  相似文献   

3.
为拓宽小麦矮秆遗传资源,利用γ射线辐照济麦22获得了一个赤霉素不敏感型矮秆突变体jm22d。株高相关性状调查结果及茎秆细胞学试验显示,jm22d株高为53±1.8 cm,比野生型(WT)低约20 cm。jm22d整株茎秆共有4节,比WT少一节且各节间长度显著小于WT。与WT相比,jm22d茎秆细胞长度缩短。赤霉素含量测定发现,jm22d叶片中赤霉素含量高于WT,而茎秆中赤霉素含量低于WT(P<0.01),因此,jm22d株高降低与赤霉素转运途径出现异常有关。为了深入研究jm22d对赤霉素的响应机理,对jm22d和WT幼苗进行赤霉素处理,分别收取处理0(D0)、1(D1)和3 d(D3)的样品进行转录组学分析。结果表明,与WT相比,在jm22d中共筛选到696个上调和1 067个下调的表达基因,其中62个和349个基因在3个时间点分别表现为上调和下调表达。叶绿素含量测定表明,jm22d中叶绿素含量随赤霉素处理时间的延长而降低,聚类分析结果表明,差异表达基因主要富集在光合作用-天线蛋白(photosynthesis-antenna proteins,ko00196)、卟啉和叶绿素代谢(porphyrin and chlorophyll metabolism,ko00860)、亚油酸新陈代谢(linoleic acid metabolism,ko00591)等通路,因此赤霉素处理对jm22d体内叶绿素含量的积累具有抑制作用。通过KEGG分析在植物激素信号转导途径中挖掘到5个差异表达基因(TraesCS2B01G582300、TraesCS2B01G600800、TraesCS2B01G556600、TraesCS2B01G630000和TraesCS6B01G439600)参与生长素、细胞分裂素等激素代谢途径,这些基因在jm22d中显著下调,这可能是jm22d矮化的重要原因。研究结果为矮秆突变体矮化机制的解析提供了重要参考。  相似文献   

4.
A dominant dwarf mutant of barley (Hordeum vulgare) that resembles dominant gibberellin (GA) "-insensitive" or "-nonresponsive" mutants in other species is described. alpha-Amylase production by endosperm half-grains of the mutant required GA3 at concentrations about 100 times that of the WT. The mutant showed only a slight growth response to GA3, even at very high concentrations. However, when additionally dwarfed, growth rate responded to GA3 over the normal concentration range, although only back to the original (dwarf) elongation rate. Genetic studies indicated that the dominant dwarf locus was either closely linked or identical to the Sln1 (Slender1) locus. A barley sequence related to Arabidopsis GAI/RGA was isolated, and shown to represent the Sln1 locus by the analysis of sln1 mutants. The dominant dwarf mutant was also altered in this sequence, indicating that it too is an allele at Sln1. Thus, mutations at Sln1 generate plants of radically different phenotypes; either dwarfs that are largely dominant and GA "-insensitive/-nonresponsive," or the recessive slender types in which GA responses appear to be constitutive. Immunoblotting studies showed that in growing leaves, SLN1 protein localized almost exclusively to the leaf elongation zone. In mutants at the Sln1 locus, there were differences in both the abundance and distribution of SLN1 protein, and large changes in the amounts of bioactive GAs, and of their metabolic precursors and catabolites. These results suggest that there are dynamic interactions between SLN1 protein and GA content in determining leaf elongation rate.  相似文献   

5.
sd1 is known as the ‘green revolution’ gene in rice because its application in rice breeding has dramatically increased rice yield. Since the ‘green revolution,’ sd1 has been extensively used to produce modern semi-dwarf varieties. The extensive use of limited dwarfing sources may, however, cause a bottleneck effect in the genetic background of rice varieties. To circumvent this problem, novel and useful sources of dwarf genes must be identified. In this study, we identified three semi-dominant dwarf mutants. These mutants were categorized as dn-type dwarf mutants according to the elongation pattern of internodes. Gibberellin (GA) response tests showed that the mutants were still responsive to GA, although at a reduced rate. Map-based cloning revealed that the dwarf phenotype in these mutants was caused by gain-of-function mutations in the N-terminal region of SLR1. Degradation of the SLR1 protein in these mutants occurred later than in the wild type. Reduced interaction abilities of the SLR1 protein in these mutants with GID1 were also observed using the yeast two-hybrid system. Crossing experiments indicated that with the use of an appropriate genetic background, the semi-dominant dwarf alleles identified in this study could be used to alleviate the deficiency of dwarfing genes for breeding applications.  相似文献   

6.
Genetics of dominant gibberellin-insensitive dwarfism in maize   总被引:20,自引:2,他引:18       下载免费PDF全文
Harberd NP  Freeling M 《Genetics》1989,121(4):827-838
D8 and Mpl1 are two dominant dwarfing mutations of maize. Although they differ in severity of dwarfism, both D8 and Mpl1 mutants are unresponsive to gibberellin (GA). Because of their close phenotypic resemblance to the recessive GA-sensitive dwarf mutants these dominant mutations may identify a gene whose product is involved in the reception of GA. With this possibility in mind we have studied the genetic properties of D8 and Mpl1. Both mutations map close to Adh1 on chromosome 1L. By marking normal and translocated 1L arms with different Adh1 electrophoretic mobility alleles, we investigated the effect of gene dosage on dominant dwarf phenotype. The results suggest that D8 and Mpl1 encode novel product functions and that these functions are relatively insensitive to the presence of the (presumed) wild-type product. Using X-ray induced chromosome breakage we created sectors of wild-type cells within D8 or Mpl1 tissue; these sectors were marked by the linked recessive lw mutation. The phenotypes of these sectors demonstrated that, at least in certain plant organs and tissues, dominant dwarfism can be an autonomous phenotype. These results are consistent with the hypothesis that the wild-type gene product acts as a GA receptor. The potential utility of dominant dwarf phenotype in plant developmental analysis is discussed, and possible mechanisms for the action of the D8 and Mpl1 mutations are considered.  相似文献   

7.
The purpose of this study was to demonstrate the metabolism of gibberellin A20 (GA20) to gibberellin A1 (GA1) by tall and mutant shoots of rice (Oryza sativa L.) and Arabidopsis thaliana (L.) Heynh. The data show that the tall and dx mutant of rice and the tall and ga5 mutant of Arabidopsis metabolize GA20 to GA1. The data also show that the dy mutant of rice and the ga4 mutant of Arabidopsis block the metabolism of GA20 to GA1. [17-13C,3H]GA20 was fed to tall and the dwarf mutants, dx and dy, of rice and tall and the dwarf mutants, ga5 and ga4, of Arabidopsis. The metabolites were analyzed by high-performance liquid chromatography and full-scan gas chromatography-mass spectrometry together with Kovats retention index data. For rice, the metabolite [13C]GA, was identified from tall and dx seedlings; [13C]GA1 was not identified from the dy seedlings. [13C]GA29 was identified from tall, dx, and dy seedlings. For Arabidopsis, the metabolite [13C]GA1 was identified from tall, ga5, and ga4 plants. The amount of [13C]GA1 from ga4 plants was less than 15% of that obtained from tall and ga5 plants. [13C]GA29 was identified from tall, ga5, and ga4 plants. [13C]GA5 and [13C]GA3 were not identified from any of the six types of plant material.  相似文献   

8.
林佳琦  李燕培  肖世祥  冯斗  禤维言 《广西植物》2022,42(11):1921-1928
香蕉的矮化突变是香蕉无性繁殖后代最常见的表型变异之一,但其变异的分子调控机理目前尚未研究清楚; 而内源赤霉素是影响植物株高的重要激素之一,GA3-氧化酶是赤霉素生物合成后期的关键酶。为探究GA3-氧化酶编码基因对香蕉矮化的分子调控机理,该研究以威廉斯B6矮化突变体及其野生型亲本为材料,通过RT-PCR技术克隆得到矮化香蕉及其野生型亲本GA3ox基因的全长cDNA序列,并对其推测的氨基酸序列进行比对分析,同时利用qRT-PCR技术对GA3ox基因在不同组织中的表达水平差异进行分析。结果表明:(1)矮化香蕉GA3ox-A和野生型香蕉GA3ox-G的ORF长度均为864 bp,均编码287个氨基酸,经序列比对分析发现两条氨基酸序列之间存在5个位点的差异,从而产生具有不同性质的蛋白质。(2)氨基酸序列同源性分析表明,矮化香蕉GA3ox的氨基酸序列与油棕、海枣、椰子的同源性最高。(3)qRT-PCR显示,GA3ox基因在矮化香蕉叶片和茎秆中的表达水平整体上低于野生型,其中GA3ox在野生型茎秆中的表达水平是矮化植株的2.2~32倍。综上推测,GA3ox基因可能对香蕉茎杆的矮化变异具有重要的调控作用。该研究结果为揭示香蕉矮化突变的分子机制与筛选优良矮化香蕉株系奠定了基础。  相似文献   

9.
10.
We characterized a spontaneous dwarf mutant showing extremely short internodes and dark green leaves originating from azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) cultivar "Erimo-shouzu." F(1) plants of 3 cross combinations between the dwarf mutant and several representative wild-type plants, Erimo-shouzu, V. angularis accession Acc2265 and wild relative V. riukiuensis accession Acc2482, supported the dwarf genotype being recessive. In a total of 3328 F(2) progeny of these 3 crosses, 65 dwarfs (2.0%) and 5 chimeric dwarfs (0.2%) segregated and the remainder were wild-type plants (97.8%). In F(3) progeny derived from self-pollinated dwarf F(2) plants, we observed wild type (54.3%), dwarf (39.1%), and chimeric dwarf (6.5%) plants. Two types of chimeric plants were observed: dwarf branches on the axils of wild-type plant stems and wild-type branches on the axils of dwarf stems. In 21 dwarf F(2) plants, the dwarf trait cosegregated with simple sequence repeat marker CEDG154 on chromosome 4. Conversely, homozygote F(2) plants at this chromosomal segment from the dwarf mutant frequently (>90%) expressed the wild-type phenotype. We concluded that the dwarf phenotype is mitotically and meiotically inheritable and controlled by a single genetically unstable locus, designated Azuki Dwarf1 (AD1), which converts between 2 phenotypic states bidirectionally.  相似文献   

11.
12.
The sensitivity of foxtail millet (Setaria italica Beauv.) dwarf mutants to GA was studied. Seedlings of dwarf mutants, Aininghuang (ANH) and CH84113 were treated with GAs (GA1, GA4, GA9, GA19 and GA20) using the micro-drop method, or by soaking in GA3 solution. Plants were also sprayed with GA3 solution at the jointing stage. It was found that ANH was a GA-responding dwarf mutant, whose leaf blade, leaf sheath and internode length increased significantly after GA application, whereas CH84113 was a non-GA-responding dwarf mutant. However, the mesocotyl in etiolated seedlings of both ANH and CH84113 responded to exogenous GA3 in a similar way. With the help of an enzyme-linked immunosorbent assay, it was found that the endogenous GA1+3 level in leaves was lower in the GA-responding dwarf mutant ANH, but higher in the non-GA-responding dwarf mutant CH84113, compared with levels in normal cultivars.  相似文献   

13.
Semi-dwarfism is an agronomically important trait in breeding for stable high yields and for resistance to damage by wind and rain (lodging resistance). Many QTLs and genes causing dwarf phenotype have been found in maize. However, because of the yield loss associated with these QTLs and genes, they have been difficult to use in breeding for dwarf stature in maize. Therefore, it is important to find the new dwarfing genes or materials without undesirable characters. The objectives of this study were: (1) to figure out the inheritance of semi-dwarfism in mutants; (2) mapping dwarfing gene or QTL. Maize inbred lines ‘18599’ and ‘DM173’, which is the dwarf mutant derived from the maize inbred line ‘173’ through 60Co-γ ray irradiation. F2 and BC1F1 population were used for genetic analysis. Whole genome resequencing-based technology (QTL-seq) were performed to map dwarfing gene and figured out the SNP markers in predicted region using dwarf bulk and tall bulk from F2 population. Based on the polymorphic SNP markers from QTL-seq, we were fine-mapping the dwarfing gene using F2 population. In F2 population, 398 were dwarf plants and 135 were tall plants. Results of χ2 tests indicated that the ratio of dwarf plants to tall plants was fitted to 3:1 ratio. Furthermore, the χ2 tests of BC1F1 population showed that the ratio was fitted to 1:1 ratio. Based on QTL-seq, the dwarfing gene was located at the region from 111.07 to 124.56 Mb of chromosome 9, and we named it rht-DM. Using traditional QTL mapping with SNP markers, the rht-DM was narrowed down to 400 kb region between SNP-21 and SNP-24. The two SNPs were located at 0.43 and 0.11 cM. Segregation analysis of F2 and BC1F1 indicated that the dwarfing gene was likely a dominant gene. This dwarfing gene was located in the region between 115.02 and 115.42 Mb on chromosome 9.  相似文献   

14.
Zou J  Chen Z  Zhang S  Zhang W  Jiang G  Zhao X  Zhai W  Pan X  Zhu L 《Planta》2005,222(4):604-612
A rice htd-1 mutant, related to tillering and dwarfing, was characterized. We show that the htd-1 mutant increases its tiller number by releasing axillary buds from dormant stage rather than by initiating more axillary buds. The dwarf is caused by averagely reducing each internode and panicle. Based on this dwarfing pattern, the htd-1 mutant could be grouped into dn-type dwarf defined by Takeda (Gamma Field Symp 16:1, 1977). In addition, the dwarfing of the htd-1 mutant was found independent of GA based on the analyses of two GA-mediated processes. Based on the quantitative determination of IAA and ABA and application of the two hormones exogenously to the seedlings, we inferred that the high tillering capacity of the htd-1 mutant should not be attributed to a defect in the synthesis of IAA or ABA. The genetic analysis of the htd-1 mutant indicated that the phenotypes of high tillering and dwarf were controlled by a recessive gene, termed htd1. By map-based cloning, the htd1 gene was fine mapped in a 30-kb DNA region on chromosome 4. Sequencing the target DNA region and comparing the counterpart DNA sequences between the htd-1 mutant and other rice varieties revealed a nucleotide substitution corresponding to an amino acid substitution from prolin to leucine in a predicted rice gene, OsCCD7, the rice orthologous gene of AtMAX3/CCD7. With the evidence of the association between the presence of one amino acid change in OsCCD7 and the abnormal phenotypes of the htd-1 mutant, OsCCD7 was identified as the candidate of the HTD1 gene.  相似文献   

15.
The dwarfing gene D-53 Is one of a few dominant genes for dwarfing In rice (Oryza satlva L.). In the present study, our genetic analysis confirmed that mutant characteristics including dwarfing, profuse tlllerlng, thin stems and small panicles are all controlled by the dominant D-53 gene. We measured the length of each Internode of KL908, a D-53-carrylng line, and classified the dwarfism of KL908 Into the dn-type. In addition, we measured elongation of the second sheath and a-amylase activity In the endosperm, and we characterized KL908 as a dwarf mutant that was neither glbberelllc acid-deficient nor glbberelllc acid-Insensitive. Using a large F2 population obtained by crossing KL908 with a wild-type variety, NJ6, the D-53 gene was mapped to the terminal region of the short arm of chromosome 11, with one simple sequence repeat marker, Ds3, co-segregating, and the other, K81114, located 0.6 cM away.  相似文献   

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17.
文章通过对所构建的水稻突变体库进行大规模筛选,获得一个稳定遗传的矮秆突变体,与野生型日本晴相比,该突变体表现为植株矮化、叶片卷曲、分蘖减少和不育等性状,命名为dtl1(dwarf and twist leaf 1)。dtl1属于nl型矮秆,激素检测表明,矮秆性状与赤霉素和油菜素内酯无关。遗传分析显示,突变性状受单一隐性核基因控制。利用dtl1与籼稻品种Taichung Native 1杂交构建F2群体,将该突变基因DTL1定位于水稻第10染色体长臂2个SSR标记RM25923和RM6673之间约70.4 kb区域内,并与InDel标记Z10-29共分离,在该区域预测有13个候选基因,但未见调控水稻株高相关基因的报道,因此,认为DTL1基因是一个新的控制水稻株高的基因。  相似文献   

18.
The most common dwarfing genes in wheat, Rht-B1b and Rht-D1b, classified as gibberellin-insensitive (GAI) dwarfing genes due to their reduced response to exogenous GA, have been verified as encoding negative regulators of gibberellin signaling. In contrast, the response of gibberellin-responsive (GAR) dwarfing genes, such as Rht12, to exogenous GA is still unclear and the role of them, if any, in GA biosynthesis or signaling is unknown. The responses of Rht12 to exogenous GA3 were investigated on seedling vigour, spike phenological development, plant height and other agronomic traits, using F2∶3 and F3∶4 lines derived from a cross between Ningchun45 and Karcagi-12 in three experiments. The application of exogenous GA3 significantly increased coleoptile length and seedling leaf 1 length and area. While there was no significant difference between the dwarf and the tall lines at the seedling stage in the responsiveness to GA3, plant height was significantly increased, by 41 cm (53%) averaged across the three experiments, in the GA3-treated Rht12 dwarf lines. Plant height of the tall lines was not affected significantly by GA3 treatment (<10 cm increased). Plant biomass and seed size of the GA3-treated dwarf lines was significantly increased compared with untreated dwarf plants while there was no such difference in the tall lines. GA3-treated Rht12 dwarf plants with the dominant Vrn-B1 developed faster than untreated plants and reached double ridge stage 57 days, 11 days and 50 days earlier and finally flowered earlier by almost 7 days while the GA3-treated tall lines flowering only 1–2 days earlier than the untreated tall lines. Thus, it is clear that exogenous GA3 can break the masking effect of Rht12 on Vrn-B1 and also restore other characters of Rht12 to normal. It suggested that Rht12 mutants may be deficient in GA biosynthesis rather than in GA signal transduction like the GA-insensitive dwarfs.  相似文献   

19.
We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of d2 was rescued by exogenous brassinolide treatment. The accumulation profile of BR intermediates in the d2 mutants confirmed that these plants are deficient in late BR biosynthesis. We cloned the D2 gene by map-based cloning. The D2 gene encoded a novel cytochrome P450 classified in CYP90D that is highly similar to the reported BR synthesis enzymes. Introduction of the wild D2 gene into d2-1 rescued the abnormal phenotype of the mutants. In feeding experiments, 3-dehydro-6-deoxoteasterone, 3-dehydroteasterone, and brassinolide effectively caused the lamina joints of the d2 plants to bend, whereas more upstream compounds did not cause bending. Based on these results, we conclude that D2/CYP90D2 catalyzes the steps from 6-deoxoteasterone to 3-dehydro-6-deoxoteasterone and from teasterone to 3-dehydroteasterone in the late BR biosynthesis pathway.  相似文献   

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