Medium reorganization energy and enzymatic reaction activation energy

Reorganization and activation energies for charge transfer reactions occurring inside a dielectric sphere have been calculated by solving the problem of polar medium reorganization within and outside a dielectric sphere placed in another infinite dielectric. The dielectric sphere is assumed to simulate a protein globule, i.e. an enzyme molecule. It has been shown that for some reaction types the activation energy tends to decrease as the globule radius increases and that for each of the reaction types considered there is an optimal globule radius an increase of which does not bring about any tangible activation energy reduction. The calculated optimal radii for different processes are in good agreement with the increasing molecular sizes in the series: ribonuclease less than or equal to lysozyme less than serine proteinases approximately equal to cysteine proteinases less than NAD-dependent dehydrogenases. The calculated radii are usually about 1.5 to 1.7 times (and molecular masses about 4-5 times) smaller than the experimental ones. The reasons for this discrepancy are discussed and it has been suggested that the approximate nature of the treatment of a protein globule as a structureless dielectric is the main reason. It is shown that charge transfer at an acute angle to the globule surface is the optimum process. For endoergonic reaction stages it is the net charge transfer towards the periphery and for exoergonic ones that in the reverse direction which are advantageous. These conclusions are consistent with the data about the structure of the above-mentioned enzymes.     

Cumulative energy demand for selected renewable energy technologies

Calculation of Cumulative Energy Demand (CED) of various energy systems and the computation of their Energy Yield Ratio (EYR) suggests that one single renewable energy technology cannot be said to be the best. Due to the difference in availability of renewable energy sources, their suitability varies from place to place. Wind energy converters, solar water heating systems and photovoltaic systems have been analysed for different types of locations. Comparing the general bandwidth of performance of these technologies, however, the wind energy converters tend to be better, followed by solar water heating systems and photovoltaic systems. Since a major part of the methodology of findingCED is very close to that of life cycle assessment and also because of the dominance of environmental impacts caused by the energy demand in the entire life cycle of any product or system, it is suggested that theCED can be used as an indicator of environmental impacts, especially in the case of power producing systems. Keywords: Cumulative energy demand; life cycle assessment; energy yield ratio; photovoltaics; solar water heating; wind energy Abbreviations: CED — Cumulative Energy Demand; EYR — Energy Yield Ratio; LCA — Life Cycle Assessment; Photovoltaics — PV; WEC — Wind Energy Converters     

Human energy

In the midst of big-oil record profits and growing debate on global warming, the Chevron Corporation launched its “Human Energy” public relations campaign. In television commercials and print advertisements, Chevron portrays itself as a compassionate entity striving to solve the planet’s energy crisis. Yet, the first term in this corporate oxymoron misleadingly reframes the significance of the second, suggesting that the corporation has a renewed focus. In depicting Chevron as a green/human organization, the “Human Energy” campaign obscures from view the corporation’s more unsightly products, policies, and practices. Reflection, however, on our own complicity in sustaining energy corporations and their activities undermines binary thinking and signals that the compulsion to denounce is insufficient. This article explores Chevron’s media campaign and one organized reaction to it. This counter-campaign both redeployed Chevron’s imagery and underscored our collusion and responsibility—tactics seeking to loosen the taut inevitability-of-oil story at Chevron’s core.     

Achieving energy neutrality in wastewater treatment plants through energy savings and enhancing renewable energy production

Wastewater treatment plants (WWTPs) consume high amounts of energy which is mostly purchased from the grid. During the past years, many ongoing measures have taken place to analyze the possible solutions for both reducing the energy consumption and increasing the renewable energy production in the plants. This review contains all possible aspects which may assist to move towards energy neutrality in WWTPs. The sources of energy in wastewater were introduced and different indicators to express the energy consumption were discussed with examples of the operating WWTPs worldwide. Furthermore, the pathways for energy consumption reductions were reviewed including the operational strategies and the novel technological upgrades of the wastewater treatment processes. Then the methods of recovering the potential energy hidden in wastewater were described along with application of renewable energies in WWTPs. The available assessment methods, which may help in analyzing and comparing WWTPs in terms of energy and greenhouse gas emissions were introduced. Eventually, successful case studies on energy self-sufficiency of WWTPs were listed and the innovative projects in this area were presented.     

Compact energy metabolism model: Brain controlled energy supply

The regulation of the energy metabolism is crucial to ensure the functionality of the entire organism. Deregulations may lead to severe pathologies such as obesity and type 2 diabetes mellitus. The decisive role of the brain as the active controller and heavy consumer in the complex whole body energy metabolism is the matter of recent research. Latest studies suggest that the brain's energy supply has the highest priority while all organs in the organism compete for the available energy resources. In our novel mathematical model, we address these new findings. We integrate energy fluxes and their control signals such as glucose fluxes, insulin signals as well as the ingestion momentum in our new dynamical system. As a novel characteristic, the hormone insulin is regarded as central feedback signal of the brain. Hereby, our model particularly contains the competition for energy between brain and body periphery. The analytical investigation of the presented dynamical system shows a stable long-term behavior of the entire energy metabolism while short time observations demonstrate the typical oscillating blood glucose variations as a consequence of food intake. Our simulation results demonstrate a realistic behavior even in situations like exercise or exhaustion, and key elements like the brain's preeminence are reflected. The presented dynamical system is a step towards a systemic understanding of the human energy metabolism and thus may shed light to defects causing diseases based on deregulations in the energy metabolism.     

Adenylate energy pool and energy charge in maturing rape seeds

A study of energy state and chemical composition of pod walls and seeds of maturing rape (Brassica napus L.) was conducted on two varieties, Victor and Gorczanski. Total adenosine phosphates, ATP, and adenylate energy charge increased with increasing cell number and cellular synthesis during the early stages, remained high at maximum dry weight accumulation and maximum substrate influx time, and decreased with ripening. A temporal control of energy supply and ATP concentration is evident in developing tissues with determined functions; whereas the association of a high energy charge and active cellular biosynthesis occurs only in tissues with a stabilized cell number.     

Restriction of energy intake, energy expenditure, and aging

Energy restriction (ER), without malnutrition, increases maximum life span and retards the development of a broad array of pathophysiological changes in laboratory rodents. The mechanism responsible for the retardation of aging by ER is, however, unknown. One proposed explanation is a reduction in energy expenditure (EE). Reduced EE may increase life span by decreasing the number of oxygen molecules interacting with mitochondria, thereby lowering reactive oxygen species (ROS) production. As a step toward testing this hypothesis, it is important to determine the effect of ER on EE. Several whole-body, organ, and cellular studies have measured the influence of ER on EE. In general, whole-body studies have reported an acute decrease in mass-adjusted EE that disappears with long-term ER. Organ-specific studies have shown that decreases in EE of liver and gastrointestinal tract are primarily responsible for initial reductions in EE with ER. These data, however, do not determine whether cellular EE is altered with ER. Three major processes contributing to resting EE at the cellular level are mitochondrial proton leak, Na(+)-K(+)-ATPase activity, and protein turnover. Studies suggest that proton leak and Na(+)-K(+)-ATPase activity are decreased with ER, whereas protein turnover is either unchanged or slightly increased with ER. Thus, two of the three major processes contributing to resting EE at the cellular level may be decreased with ER. Although additional cellular measurements are needed, the current results suggest that a lowering of EE could be a mechanism for the action of ER.     

Automated activation energy

A method and equipment used for automated determination of activation energies on a single sample are described. Essentially identical results are obtained in both automated and manual methods. The automated method is particularly valuable for minimizing the amounts of enzyme, substrate, and time required. Further, errors in repetitive pipetting and calculation are eliminated.     

AMPK: a nutrient and energy sensor that maintains energy homeostasis

AMP-activated protein kinase (AMPK) is a crucial cellular energy sensor. Once activated by falling energy status, it promotes ATP production by increasing the activity or expression of proteins involved in catabolism while conserving ATP by switching off biosynthetic pathways. AMPK also regulates metabolic energy balance at the whole-body level. For example, it mediates the effects of agents acting on the hypothalamus that promote feeding and entrains circadian rhythms of metabolism and feeding behaviour. Finally, recent studies reveal that AMPK conserves ATP levels through the regulation of processes other than metabolism, such as the cell cycle and neuronal membrane excitability.     

The species–energy theory: a role for energy variability

Species–energy theory posits that energy availability regulates population sizes, extinction rates and ultimately species richness. This theory has focused mostly on total energy as a measure of energy availability. However, because energy variation can also influence population sizes and extinction rates, species–energy theory should arguably consider simultaneously both total energy and its variation. Using data on species richness of land birds and mammals, we compared the fit of three species–energy models including total energy, energy variation or both combined. We show that the combination of total energy and energy variation has greater predictive power than any of them considered separately. We also evaluate three crucial assumptions of this modified species–energy theory and show that they are supported by available data. These results illuminate the current debate on climate change, given that both average conditions and variability of climatic conditions are likely to change in the future.     

Primary cortical neuronal cultures reduce cellular energy utilization during anoxic energy deprivation

It has been widely hypothesized that neurons reduce cellular energy use in response to periods of energy deprivation. To test this hypothesis, we measured rates of energy use under normoxia and anoxia in immature (6 days in vitro) and mature (13 days in vitro) neuronal cultures. During anoxic incubation immature and mature cultures reduced cellular energy use by 80% and 45%, respectively. Reduced cellular energy use dramatically affected ATP depletion in neuronal cultures under anoxia. Intracellular ATP stores were expected to deplete within 3 min of anoxia. However, ATP was maintained at decreased but stabilized concentrations for at least 3 h. The capacity of neuronal cultures to reduce cellular energy use during anoxia correlated with their sensitivity towards simulated ischemia. Immature cultures, with the largest capacity to reduce cellular energy use, survived simulated ischemia 2.5 times longer than mature cultures. The addition of glutamate receptor antagonists to mature cultures further decreased cellular energy use during anoxia and significantly extended their survival time under simulated ischemia. This study verifies that primary cortical neuronal cultures reduce cellular energy use during energy deprivation. Additionally, we show that maturation of glutamate receptor activity increases non-depressible energy demand in neuronal cultures.     

Conversion of the energy of a high-current REB into electromagnetic wave energy

Results are presented from a theoretical investigation and quantitative analysis of the physical processes that govern the efficiency of a coaxial device aimed at converting the energy of a relativistic electron beam into the energy of a TEM wave (a wave in a circular cylindrical coaxial waveguide). The key diffractional problem is solved exactly using a simplified theoretical model, which makes it possible to understand the mechanisms for the formation of a TEM wave and determine how the beam parameters and the design parameters of the converter affect the relative fractions of the kinetic energy of a relativistic electron beam and the energy of its own magnetic and electric fields that are transferred into the energy of the TEM wave field. The results obtained are analyzed quantitatively, and prospects for further theoretical and experimental research in this area are outlined.     

Adiponectin plays an important role in efficient energy usage under energy shortage

Adiponectin is an adipose tissue-specific secretory protein known to be an insulin-sensitizing protein. In this study, we generated adiponectin sense and antisense transgenic (Tg) mice to investigate whether adiponectin plays a role in the regulation of energy homeostasis during the growth stage. Spontaneous motor activity of antisense Tg mice were markedly reduced during fasting, particularly in young female mice, compared with wild type (Wt) and sense Tg mice. Furthermore, both body weight and adipose tissue mass of the antisense female Tg mice drastically reduced during fasting. To examine the relationship between the collapse of abdominal white adipose tissue (WAT) and serum adiponectin level, we measured the expression of genes related to energy expenditure, such as uncoupling protein (UCP). Notably, the mRNA of UCP1 in the WAT of antisense Tg female mice was markedly less than that of Wt mice and the UCP1 mRNA was strongly increased during fasting. These findings suggest that the serum adiponectin is important to maintaining energy homeostasis under energy shortage conditions, such as over female pubertal development.     

Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering

The need to develop and improve sustainable energy resources is of eminent importance due to the finite nature of our fossil fuels. This review paper deals with a third generation renewable energy resource which does not compete with our food resources, cyanobacteria. We discuss the current state of the art in developing different types of bioenergy (ethanol, biodiesel, hydrogen, etc.) from cyanobacteria. The major important biochemical pathways in cyanobacteria are highlighted, and the possibility to influence these pathways to improve the production of specific types of energy forms the major part of this review.     

Scaling nestling energy requirements

The total energy metabolized (TME) by nestling birds, from hatching to fledging, scales as M ^1.06, with body-mass at fledging (M) explaining 97% of the variation in TME. After statistically removing the effects of body-mass, multiple-regression analysis reveals that 69% of the variation in TME is explained by nestling developmental rate, expressed as the time to fledging ( t _fl, days). Together t _fl and M explain 99% of the variation in TME for the 30 species considered ( M range 9.7 to 3700 g).
Peak daily metabolized energy (DME, kJ/day), the maximum rate at which parents must provision their nestlings, scales with fledging mass as M^0.78. Body-mass explains 96% of the variation in nestling peak DME, with 37% of the residual variation being attributable to the time taken to fledge ( t _fl). Together, t _fl and M explain 97% of the variation in peak DME. An examinination of residuals indicates that adaptive modifications in nestling energetics are attained principally through changes in growth rate, which affect TME and peak DME oppositely. Doubling nestling growth rate increases peak DME by 61%, but decreases total nestling energy demand (TME) by 77%. This opposing effect complicates evolutionary interpretations of avian reproductive patterns.