COMPARATIVE DEMOGRAPHICS OF A GENERALIST PREDATORY LADYBIRD ON FIVE APHID PREY： A LABORATORY STUDY

Prey driven demographic parameters of an aphidophagous ladybird, Propylea dissecta (Mulsant) were studied in attempt to answer how ladybird overcomes the problem of seasonally fluctuating food base. The ladybird reared for five generations in laboratory derived differential nutrition from five food sources (i.e. aphid species, Rhopalosiphum maidis (Fitch), Aphis craccivora Koch, Aphis gossypii Glover, Uroleucon compositae (Theobald) and Lipaphis erysimi (Kalt.)). The order of relative prey suitability was A. gossypii, A. craccivora, R. maidis, U. compositae and L.erysimi. Neonates suffered maximum mortality followed by eggs. Estimates of net reproductive rate,intrinsic rate of increase and finite rate of increase were highest (407.18, 0.2274 day^-1, 1.2553 day^-1) on A. gossypii and lowest (176.02, 0.1533 day^-1, 1.1657 day^-1) on L. erysimi. Generation time was shortest (26.43 days) on A. gossypii and longest (33.73 days) on L. erysimi. The present study thus,provides an explanation to the high incidence of P. dissecta on A. gossypii, as it experienced high intrinsic rate of increase and optimal values for related demographic parameters.     

温度对八斑鞘腹蛛实验种群生长的影响

八斑鞘腹蛛(Coleosoma octomaculatum)是农田害虫的主要天敌之一。它分布范围广、数量大,可捕食棉蚜(Aphis gossypii)、豆蚜(A.craccivora)、柳蚜(A.saliceti)、菜缢管蚜(Lipaphis erysimi)、麦长管蚜(Macrosi-     

异色瓢虫对豆蚜的捕食功能反应

试验开展了异色瓢虫Harmonia axyridis Pallas对豆蚜Aphis craccivora Koch的捕食功能反应研究。结果表明,异色瓢虫各虫态的功能反应类型均属于HollingⅡ型。随着豆蚜数量的上升,异色瓢虫对豆蚜的捕食增加量逐渐减小直至趋于稳定。异色瓢虫各龄幼虫、成虫对豆蚜的寻找效应均随豆蚜数量的增加而减小。异色瓢虫4龄幼虫的a/Th值最大,为2255.67,捕食功能最强,其后为成虫、3龄、2 龄、1龄幼虫。     

Effect of cotton aphids feeding on transgenic cotton varieties on the behavior of Propylaea japonica

本文以GK12、33B和SGK3213种转基因棉和对应的3种常规棉泗棉3号、33及石远321为材料,通过室内测定,研究了取食转基因棉的棉蚜Aphis gossypii Glover对龟纹瓢虫Propylaea japonica Thunberg1～4龄幼虫功能反应的影响.结果指出,龟纹瓢虫对来自转基因棉田蚜虫的捕食量大多高于常规棉田,且捕食量随猎物密度的增加而增大.在1、2龄幼虫期,龟纹瓢虫取食转双价基因棉田蚜虫的数量多高于取食转单价基因棉田的蚜虫数量,但是在3、4龄幼虫时,龟纹瓢虫取食转双价棉田蚜虫的数量多低于取食转单价棉田的蚜虫数量.龟纹瓢虫对来自转基因棉田棉蚜的捕食功能反应符合HollingⅡ型.龟纹瓢虫1龄幼虫取食GK12和SGK321 2个转基因棉田蚜虫的最大日捕食量大于对照,处理时间短于对照;龟纹瓢虫P.japonica 2、3龄幼虫取食33B和SGK321 2个转基因棉田蚜虫A.gossypii的日最大捕食量大于对照,处理时间短于对照;4龄时取食转基因棉田蚜虫的数量都大于对照,处理时间均短于对照,其中取食转双价基因棉SGK321棉田蚜虫的日最大捕食量小于取食单价棉田蚜虫的日最大捕食量.     

十一星瓢虫对枸杞棉蚜的捕食作用研究

十一星瓢虫Coccinella undecimpunctata Linnaeus是青海省柴达木地区的一种重要捕食性天敌。为明确其对枸杞棉蚜Aphis gossypii Glover和枸杞木虱Poratrioza sinica Yang et Li.两种主要枸杞害虫的嗜好性,开展了捕食选择性试验,发现当两种害虫共存时,瓢虫成虫更喜食棉蚜。为进一步探究十一星瓢虫对枸杞棉蚜的捕食能力,测定了瓢虫各龄幼虫和成虫在不同棉蚜密度下的捕食量、寻找效应以及种内干扰、自身密度对捕食作用的影响。结果表明：十一星瓢虫各虫态对棉蚜的捕食功能反应均符合Holling-Ⅱ功能反应模型,种内干扰、自身密度对捕食量的影响分别符合Hassell干扰效应模型和Watt模型。十一星瓢虫1~4龄幼虫及成虫对枸杞棉蚜的日最大理论捕食量分别为18.94、44.29、81.57、145.27和126.48头;在一定空间、相同瓢蚜比条件下,瓢虫同一虫态个体间存在种内干扰效应,以1龄幼虫受干扰最大,干扰常数为1.1803。此外,瓢虫自身密度对其捕食作用产生一定影响,以4龄幼虫和成虫尤甚,竞争参数分别为0.7948、0.6848。本研究可为今后更高效地利用青海高原本地十一星瓢虫资源提供理论依据。     

多异瓢虫食物消耗与利用与其猎物棉蚜的寄主植物种类相关

【目的】猎物质量和类型在捕食者食物选择、消耗及营养转化过程中起着重要作用,植物的营养质量影响植食者的营养质量,进而植食者作为捕食者猎物的营养质量。为进一步了解植物-猎物-捕食者三营养层的相互作用,研究了寄主植物对捕食者食物消耗与利用的影响。【方法】在室内评价了多异瓢虫Hippodamia variegata (Goeze) 4龄幼虫和雌成虫取食和利用 5种不同葫芦科植物上的棉蚜 Aphis gossypii Glover的效率。【结果】多异瓢虫4龄幼虫和成虫均对南瓜Cucurbita moschata var. melonaeformis上的蚜虫取食量最高,而对瓢葫芦Lagenaria siceraria var. gourda上的蚜虫取食量最低。4龄幼虫取食哈密瓜 Cucumis melo var. cantalupensis上的蚜虫时,其相对生长率、食物利用率和食物转化率均达到最大,而雌成虫在取食黄瓜Cucumis sativus和搅瓜Cucurbita pepo var. medullosa上的蚜虫时,其相对生长率不存在显著差异,在取食黄瓜、瓢葫芦和搅瓜上的蚜虫时,其食物利用率和食物转化率也不存在显著差异。因此,哈密瓜上的瓜蚜更适宜作为多异瓢虫4龄幼虫的猎物,而黄瓜、瓢葫芦和搅瓜上的瓜蚜更适宜作为成虫的猎物。【结论】寄主植物种类与多异瓢虫对棉蚜的捕食效率相关,该结论为进一步利用昆虫的食性对害虫进行控制奠定理论基础。     

北疆棉田捕食性天敌昆虫应对棉蚜的数量反应

【目的】棉蚜Aphis gossypii是新疆棉田的主要害虫,近来的研究多集中于南疆棉区,较少关注北疆棉田在新种植模式下的天敌种群数量发生规律。本文研究了北疆棉田棉蚜的优势种捕食性昆虫数量发生规律。【方法】对五家渠地区棉田中棉蚜及其4种主要捕食性天敌昆虫(2种瓢虫和2种草蛉)的种群数量进行了持续2年的跟踪调查。【结果】不同种捕食性天敌昆虫对棉蚜种群数量变化具有不同的响应,具体表现为:七星瓢虫Cocinella septempunctata数量随蚜虫数量增大而显著增大,多异瓢虫Hippodamia verigata和普通草蛉Chrysopa carnea随蚜虫数量增大而逐渐增大,而大草蛉Ch.septempunctata种群数量与棉蚜数量无关;连续2年的大田调查均发现,这4种捕食性昆虫种群数量在7月底有一高峰,而且七星瓢虫和大草蛉数量大于多异瓢虫和普通草蛉。【结论】七星瓢虫和大草龄是北疆五家渠地区棉田棉蚜的优势天敌。     

多异瓢虫食物消耗与利用与其猎物棉蚜的寄主植物种类相关（英文）

【目的】猎物质量和类型在捕食者食物选择、消耗及营养转化过程中起着重要作用,植物的营养质量影响植食者的营养质量,进而植食者作为捕食者猎物的营养质量。为进一步了解植物-猎物-捕食者三营养层的相互作用,研究了寄主植物对捕食者食物消耗与利用的影响。【方法】在室内评价了多异瓢虫Hippodamia variegata(Goeze)4龄幼虫和雌成虫取食和利用5种不同葫芦科植物上的棉蚜Aphis gossypii Glover的效率。【结果】多异瓢虫4龄幼虫和成虫均对南瓜Cucurbita moschata var.melonaeformis上的蚜虫取食量最高,而对瓢葫芦Lagenaria siceraria var.gourda上的蚜虫取食量最低。4龄幼虫取食哈密瓜Cucumis melo var.cantalupensis上的蚜虫时,其相对生长率、食物利用率和食物转化率均达到最大,而雌成虫在取食黄瓜Cucumis sativus和搅瓜Cucurbita pepo var.medullosa上的蚜虫时,其相对生长率不存在显著差异,在取食黄瓜、瓢葫芦和搅瓜上的蚜虫时,其食物利用率和食物转化率也不存在显著差异。因此,哈密瓜上的瓜蚜更适宜作为多异瓢虫4龄幼虫的猎物,而黄瓜、瓢葫芦和搅瓜上的瓜蚜更适宜作为成虫的猎物。【结论】寄主植物种类与多异瓢虫对棉蚜的捕食效率相关,该结论为进一步利用昆虫的食性对害虫进行控制奠定理论基础。     

高温胁迫六斑月瓢虫对其生物学特性及其捕食作用的影响

【目的】为明确高温胁迫下对六斑月瓢虫Menochilussexmaculates的生物学特性及其捕食作用的影响。【方法】在35℃高温处理不同时长(3、6、9 h)的胁迫下,探究六斑月瓢虫的生长发育、存活、繁殖力和虫态特征的变化规律,同时评估了其对豆蚜Aphis craccivora的捕食功能与寻找效应。【结果】与25℃饲喂相比,高温胁迫下六斑月瓢虫的幼期历期和雌雄虫寿命随着胁迫时长的增加而缩短,且高温胁迫不同程度降低了六斑月瓢虫的产卵量、卵孵化率和幼期存活率,而对六斑月瓢虫4龄幼虫、成虫的体长和虫重无影响。通过研究捕食作用,与常温25℃相比,35℃高温胁迫在一定程度上促进了六斑月瓢虫对蚜虫的捕食效能,其中在高温胁迫9 h后,其捕食能力(a'/Th)和寻找效应(S)均高于3 h和6 h的。【结论】高温对六斑月瓢虫的生物学特性产生负面的影响,而对其捕食活动具有正面的影响。研究结果为六斑月瓢虫的生物防治提供了科学依据,同时为全球气候变暖下天敌昆虫的保护与利用奠定了基础数据。     

天敌体内玉米蚜的CoⅡ标记检测

以玉米蚜Rhopalosiphum maidis Fitch及玉米田的捕食性天敌为研究对象,筛选1对特异扩增玉米蚜细胞色素氧化酶Ⅱ(COⅡ)基因序列片段的引物。种特异性检验表明,该引物只对玉米蚜基因组DNA具有扩增效果,对与其同域发生的其他种类不具扩增作用;室内以该引物检测喂食单头玉米蚜的异色瓢虫Harmonia axyridis(Pallas)成虫和大草蛉Chrysopa pallens(Rambur)成虫腹内靶标食物的衰变情况,结果表明,异色瓢虫成虫和大草蛉成虫阳性比率的半衰期分别为喂食后2·814和2·441h;对玉米田间捕食性天敌的检测结果表明,在所检测的6类群13种捕食性天敌中,大灰食蚜蝇幼虫、草蛉幼虫、异色瓢虫成虫和幼虫、龟纹瓢虫成虫和幼虫对玉米蚜的捕食作用检出率较高,大于40%。     

COMPARATIVE DEMOGRAPHICS OF A GENERALIST PREDATORY LADYBIRD ON FIVE APHID PREY: A LABORATORY STUDY

Abstract  Prey driven demographic parameters of an aphidophagous ladybird, Propylea dissecta (Mulsant) were studied in attempt to answer how ladybird overcomes the problem of seasonally fluctuating food base. The ladybird reared for five generations in laboratory derived differential nutrition from five food sources ( i.e . aphid species, Rhopalosiphum maidis (Fitch), Aphis craccivora Koch, Aphis gossypii Glover, Uroleucon compositae (Theobald) and Lipaphis erysimi (Kalt.)). The order of relative prey suitability was A. gossypii, A. craccivora, R. maidis, U. compositae and L. erysimi . Neonates suffered maximum mortality followed by eggs. Estimates of net reproductive rate, intrinsic rate of increase and finite rate of increase were highest (407. 18, 0.2274 day^-1, 1.2553 day^-1) on A. gossypii and lowest (176.02, 0.1533 day^-1, 1.1657 day^-1) on L. erysirni . Generation time was shortest (26.43 days) on A. gossypii and longest (33.73 days) on L. erysirni . The present study thus, provides an explanation to the high incidence of P. dissecta on A. gossypii , as it experienced high intrinsic rate of increase and optimal values for related demographic parameters.     

Quality of different aphids as hosts of the parasitoid Lysiphlebus testaceipes (Cresson) (Hymenoptera: Braconidae, Aphidiinae)

Lysiphlebus testaceipes (Cresson) has a broad aphid host range; however the quality of these preys may interfere in its biological feature. This study aimed to evaluate the quality of three Macrosiphini, Brevicoryne brassicae (L.), Lipaphis erysimi (Kaltenbach) and Myzus persicae (Sulzer), and three Aphidini Schizaphis graminum (Rondani) Rhopalosiphum maidis (Fitch) and Aphis gossypii Glover as hosts to L. testaceipes and to determine the relation possible of host preference, of size and quality of the host. The tests were carried out in climatic chamber at 25 +/- 1 degrees C, RH 70 +/- 10% and 12h photophase. The parasitoid did not oviposite in B. brassicae and L. erysimi, while the other species were nutritionally suitable to the parasitoid. L. testaceipes showed preference for aphids from tribe Aphidini and these hosts presented better quality to the parasitoid when compared to Macrosiphini. Interactions among size, preference and quality between the Aphidini were found. L testaceipes showed preference (parasitism rate 76.7%) for R. maidis, the bigger host (hind tibia with 0.281 mm). This host provided bigger size (hind tibia with 0.49 mm) and higher emergence rate (95.6%) to the parasitoid when compared to A. gossypii (parasitism rate of 55.7%). Also the smaller host A. gossypii (0.266 mm) provided smaller size hind tibia (0.45 mm) and higher mortality of the parasitoid (emergence rate 72.1%). However, the development time was shorter and the longevity was higher in A. gossypii (6.3 and 5.4 days, respectively) when compared to the host R. maidis (6.7 and 3.8 days, respectively), and not been related to host size.     

Temperature-dependent life attributes of an aphidophagous ladybird, Propylea dissecta

Reproductive attributes, age-specific fecundity and natality based life-tables of an aphidophagous ladybird, Propylea dissecta (Mulsant) were investigated at five temperatures, using aphid, Aphis gossypii as prey. Pre-oviposition period decreased significantly with increase in temperature up to 27°C and thereafter increased at a slower rate. Egg viability dropped significantly at temperature extremes. Females lived longer than males; however, longevity decreased significantly with increase in temperature. The predicted total fecundity and oviposition rate were 952.54 eggs and 35.15 eggs per day at 27°C predicted by a mathematical model compared to empirical estimates of 856.00±30.00 eggs and 39.7±2.26 eggs per day. Age-specific fecundity was triangular and temperature dependent. The peak in oviposition rate occurred earlier at higher temperatures. The highest values of net reproductive rate (R_o=431.1), intrinsic rate of increase (r_m=0.2134 day^-1) and finite rate of increase (λ=1.2379 day^-1) were recorded at 27°C, which suggests it is the optimum temperature for the mass rearing of P. dissecta.     

Prey-Dependent Life Attributes of an Aphidophagous Ladybird Beetle, Propylea dissecta (Coleoptera: Coccinellidae)

Predation potential, development, immature survival and reproduction of an aphidophagous ladybeetle, Propylea dissecta (Mulsant) was studied when fed on seven aphid prey, viz. Aphis gossypii, Aphis craccivora, Lipaphis erysimi, Uroleucon compositae, Brevicoryne brassicae, Rhopalosiphum maidis and Myzus persicae. A. gossypii was most suitable and consumed by the larvae and adults of P. dissecta, while M. persicae, the least. Pre-imaginal development of P. dissecta was fastest (0.080 day^-1) when A. gossypii was used as prey, whilst slowest (0.061 day^-1) on M. persicae. The immature survival, adult emergence, adult male and female longevity of P. dissecta was maximal (i.e., 77.10±0.04 and 93.21±0.79%, 57.10±1.62 and 62.40±1.93 days, respectively) on A. gossypii and minimal (i.e., 63.01±1.87 and 81.73±1.79%, 42.50±1.21 and 49.40±2.32 days, respectively) when M. persicae was provided as prey. Oviposition period, fecundity, percent egg viability and mean reproductive rate was maximum (i.e., 50.30±2.03 days, 856.00±30.00 eggs, 96.40±0.31% and 17.02 eggs per day) on A. gossypii, and minimum (i.e., 18.00±1.40 days, 212.00±18.21 eggs, 72.46±2.81% and 11.78 eggs per day) on M. persicae. Adult weight and developmental rate of P. dissecta have a positive correlation, which suggests that if immature stages of ladybeetle developed faster, they should grow into heavier adults. Female longevity and fecundity also have a positive correlation. The findings also reveal that all seven aphid species tested are essential food. Rank order of prey species was consistent in all experimental parameters.     

Influence of six aphid prey species on development and reproduction of a ladybird beetle, Coccinella septempunctata

Pre-imaginal development, immaturesurvival, and reproduction of a ladybird, Coccinella septempunctata Linnaeus, werestudied in response to six aphid species, Aphiscraccivora Koch, Aphis gossypii Glover,Aphis nerii Boyer de Fonscolombe,Lipaphis erysimi (Kaltenbach), Myzuspersicae (Sulzer) and Uroleuconcompositae (Theobald) to quantify theirrelative suitability as prey. Pre-adultdevelopment was shortest (13.93 ± 0.12 days)when fed on L. erysimi and longest(22.85 ± 0.10 days) on A. nerii. Immaturesurvival, adult emergence, growth index,relative growth rate, development rate, maleand female longevity, oviposition period,fecundity and hatching percent were maximal, i.e. 73.47 ± 0.89%, 90.07 ± 1.43%,8.62 ± 0.23, 1.52 ± 0.02, 0.07,81.10 ± 1.26 days, 85.70 ± 1.45 days,69.80 ± 1.32 days, 1764.10 ± 8.46,and 87.88 ± 1.05, respectively when C.septempunctata were fed on L. erysimi.The same parameters were minimal, i.e.43.86 ± 1.33%, 71.65 ± 2.75%,2.02 ± 0.08, 0.49 ± 0.02, 0.04,44.40 ± 1.39 days, 53.50 ± 1.00 days,16.40 ± 0.60 days, 203.20 ± 11.83, and48.68 ± 2.06, respectively on A. nerii. Theweights of different ladybird life stages weremaximal after feeding on L. erysimi and minimalon A. nerii. Regression analyses of thedata revealed linear relationships betweendevelopment rate and weight of adult; dailyprey consumption and relative growth rate; logweight of adult male and female; and longevityand fecundity of female. On the basis of thesefindings, the order of suitability of aphidspecies for C. septempunctata is L. erysimi >M. persicae > A. craccivora > A. gossypii >U. compositae > A. nerii. Thus, the presentinformation can be utilized for the massrearing of C. septempunctata by supplyingthe best food and can also help in theprediction of the relative abundance of theladybird on different aphid infestations in thefields.     

萝卜蚜和豆蚜染色体C-带带型比较与分析

本研究利用C-带技术,显示出萝卜蚜和豆蚜的染色体C-带带型,并进行了初步分析。萝卜蚜与豆蚜的染色体在组型上只有第4对染色体稍有区别;在带型上两者有很明显的差异。萝卜蚜有2对散漫着丝粒染色体,豆蚜没有该种染色体。豆蚜的第4对染色体在端部缺少-C带带纹。萝卜蚜的第2对染色体与豆蚜的第2对染色体带型相似。根据萝卜蚜和豆蚜的C-带带型,讨论了二者在进化中的亲缘关系。     

Reproductive performance of four aphidophagous ladybirds on cowpea aphid, Aphis craccivora Koch

Abstract:  This study investigated prey consumption, egg production, percent progeny loss, reproductive, pre- and post-reproductive periods, reproductive time ratio, reproductive rate and bioconversion efficiency of four aphidophagous ladybirds, viz. Cheilomenes sexmaculata (Fabricius), Coccinella septempunctata Linnaeus, Coccinella transversalis Fabricius and Propylea dissecta (Mulsant) on Dolichos lablab Linnaeus infested with cowpea aphid, Aphis craccivora Koch. C. sexmaculata had the highest bioconversion efficiency, reproductive rate and reproductive time ratio followed in rank order by P. dissecta , C. transversalis and C. septempunctata . This study indicates that C. sexmaculata has a narrow ecological relationship with A. craccivora . The increased allocation of resources to reproduction as indicated through a high reproductive time ratio and high bioconversion efficiency of C. sexmaculata and P. dissecta suggest that they may be better adapted to compete for this prey with larger species like C. transversalis and C. septempunctata .     

The use of honeydew in foraging for aphids by larvae of the ladybird beetle, Coccinella septempunctata L. (Coleoptera: Coccinellidae)

Abstract.  1. To clarify the use of honeydew in foraging for aphids by larvae of the ladybird beetle, Coccinella septempunctata L., searching behaviour of ladybird larvae for Aphis craccivora Koch and Acyrthosiphon pisum Harris and the abundance of honeydew under aphid colonies were examined in laboratory experiments.
2. More larvae responded by climbing the plants with aphids than responded to plants without aphids. When the plants were replaced with sticks, in order to exclude visual and olfactory cues from plants and aphids, more larvae of C. septempunctata climbed sticks above the area that contained honeydew than climbed sticks above the area that did not contain honeydew. Then, ladybird larvae use honeydew as a contact kairomone when foraging for aphids.
3.  Aphis craccivora deposited a larger number of honeydew droplets beneath the plants than did similar numbers of A. pisum. Thus, C. septempunctata larvae licked more frequently the honeydew of A. craccivora than that of A. pisum and spent longer searching on the area containing honeydew of A. craccivora than that of A. pisum . Consequently, a larger number of larvae climbed a stick above honeydew of A. craccivora than that of A. pisum.
4. It may be also considered that C. septempunctata larvae can distinguish honeydew of the two aphid species and respond more strongly to A. craccivora than A. pisum.     

植物乙醇提取物对蔬菜蚜虫和蚜茧蜂的影响

用药膜法测定8种常见植物的乙醇提取物对桃蚜(Myzus persicae)、瓜蚜(Aphis gossypii)和萝卜蚜(Lipaphis erysimi)的忌避和控制作用,以及时蚜虫重要天敌--菜蚜茧蜂(Aphidius gifuensis)和菜少脉蚜茧蜂(Diaeretiella rapae)存活的影响．结果表明,供试的8种植物乙醇提取物对3种蚜虫都有一定的忌避作用,其中效果最好的是白花非洲山毛豆(Tephrosia vogelli)和樟树(Cinnamomum camphora),对桃蚜、瓜蚜和萝卜蚜的忌避率分另达0、414、0．729、0．547和0、549、0．690、0．729;除黄素馨(Jasminum mesuyi)和草胡椒(Peperomia pellucida)外,其余6种植物乙醇提取物对桃蚜、瓜蚜、萝卜蚜都有较好的控制作用,其中山毛豆、樟树对蚜虫的控制效果最佳,鸡矢藤(Paederia scandena)和芒箕(Dicranopteris dichotoma)仅次于前2种植物,白兰花(Michelia alba)对瓜蚜和萝卜蚜也有较好的控制作用;鱼藤精稀释1000倍对萝卜蚜效果很好,对桃蚜控制作用较差．8种提取物中,山毛豆对蚜茧蜂有较强的毒性,4h死亡率达63．33％,仅次于鱼藤酮精稀释100倍(4h的死亡率达80％),而樟树、羊蹄甲(Bauhinia variegata)、黄素馨、白兰花和草胡椒的乙醇提取物与对照没有差异,对蚜茧蜂安全、无毒．     

Biological control of Aphis gossypii on pepper plant using Coccinella algerica

To calibrate biological control in pepper plant against Aphis gossypii using Coccinella algerica different experimentation were made. The consumption of C. algerica during the larval stage was enumerated in laboratory conditions. The densities (1, 4, 8 larvae/plant) to L2 stage of C. algerica necessarily to decrease aphid's populations were determined in plant pepper and the daily consumption in the pepper plant at the different densities was calculated. The density with 8 larvae at semi natural conditions is higher significantly. In protected crop of pepper, different releases to C. algerica were made with eggs, second stage larva and with ladybird before infestation with A. gossypii. A strategy to introduce L2 stage is preferred than the release with punters of C. algerica at different densities. At the same, the Introduce L2 stage is compared to release the C. algerica ladybird before the infestation of A. gossypii in the protected crops. The activity of ladybird has been maintained in the protected crop and it was shown when the infestation appeared.