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柑桔近缘植物酯酶同工酶的研究 总被引:2,自引:0,他引:2
廖映Fen 《Acta Botanica Sinica》1988,30(2):163-168
本文用聚丙烯酰胺凝胶电泳测定了柑桔近缘植物14个种群的种子及幼苗的酯酶同工酶,根据酶谱及扫描图的异同,分析了彼此的亲缘关系,试验结果表明,柑桔近缘植物种属间的酯酶同工酶的酶带数目,酶活性,迁移率及酶谱扫描均有不同程度的差异,同一品种不同发育时期的同工酶也具有不同表现形式,特别是柑桔种子的酯酶同工酶谱一般较稳定,可以作为柑桔亲缘关系的生化遗传指标。 相似文献
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赖草属七个种同工酶研究 总被引:5,自引:0,他引:5
用聚丙酰胺凝胶电泳法对赖草属7个种的幼根、幼叶进行了酯酶、过氧化物酶同工酶分析,结果表明:无论从相同器官进行不同的同工酶分析,还是从不同器官进行相同的同工酶分析,这7个种的酶谱在各带区存在相似酶带,但更多的是相异酶带。从酯酶、过氧化物酶这两种酶分析结果来看,酯酶比过氧化物酶分离效果好些;从幼根和幼叶这两个器官的酶谱来看,幼根比幼叶酶带多些,分离效果也好些。同时也表明这7个种的酶谱变化与染色体倍性变化无关 相似文献
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缘蝽科四种昆虫同工酶鉴别(半翅目:缘蝽科) 总被引:7,自引:0,他引:7
本研究运用聚丙烯酰胺垂直板电泳技术对缘蝽科4个种的酯酶同工酶进行了检测,酯酶酶谱可分为4个区,酯酶酶谱在属间表现明显差异,在属内具有某些共性,聚类分析显示4个种的分类地位,并说明性别差异大于个体差异但小于种间差异。 相似文献
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Esterase isozyme of dry seeds, anthers and immature seeds from 54 accessions of indigenous wheat to China was analysed. The result showed the specialties of esterase isozyme in different tissues. There were 12 zymogram types of esterase isozyme in 54 materials. A special marked band, esterase isozyme band 2A, was found in Xinjiang wheat. Cluster analysis suggested that (1) Yunnan wheat was geneticaly close related to Tibet semi-wild wheat. Both of them are in the same level of evolution and belong to one taxonomical group. (2) Common wheat and Tibet common wheat belong to one group and (3) Xinjiang wheat is a special group, having its own evolutionary process. 相似文献
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采用聚丙烯酰胺凝胶电泳法对中国几种特殊普通小麦的幼芽进行了酯酶同工酶分析。结果表明:同种不同来源的麦酶谱差异不大,较整齐一致,只有个别材料有酶带的增减及沾性强弱有别;对于不同的物种云南铁壳麦与西藏半野生小麦的酶谱很相近似,属同一类群,新疆稻麦慢带较模糊,少第3条酶带归属别一类群。 相似文献
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亚麻耐盐性愈伤组织的生理生化特性 总被引:4,自引:0,他引:4
以亚麻(双亚5号)耐盐愈伤组织为材,以同种亚麻普通愈伤组织为对照,分别经含0~250 mmol·L-1 NaC1的培养基培养20 d后,比较含水量、MDA含量、POD酶活、SOD酶活、EST同工酶谱、POD同工酶谱及SOD同工酶谱。结果表明:亚麻耐盐组愈伤组织与对照组愈伤组织在多方面存在明显差异,前者含水量均高于后者;丙二醛含量也高于后者,但变化幅度不大;POD酶活均高于对照组;酯酶同工酶谱不同于对照组,酯酶酶活量均高于对照组;POD同工酶谱比对照组多一条带,且酶活均高于对照组;SOD同工酶谱与对照组一样多,但酶活均高于对照组,且平稳。这为进一步筛选亚麻耐盐突变细胞系奠定了良好的基础。 相似文献
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6个杏鲍菇菌株生理生化研究 总被引:2,自引:0,他引:2
目的:对收集的6个杏鲍菇菌种菌丝进行生理、生化研究,酯酶同工酶法分析菌株间的差异与关系。方法:利用杏鲍菇菌丝阶段的生长速度测定、诱变剂抗性试验和酯酶同工酶比较,对杏鲍菇菌株营养生长阶段的理化指标进行研究,对酯酶同工酶谱进行相似系数和遗传距离分析,确立各菌株的异同。结果:菌株间菌丝生长速度差异不显著;抗诱变剂实验结果显示,2、5、6号菌株对诱变剂抗性较强;酯酶同工酶分析表明,杏鲍菇6个菌株同工酶酶带存在的差异很大。结论:通过试验表明,所选6株杏鲍菇菌种为各自独立菌株。 相似文献
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木耳栽培菌株酯酶同工酶的酶谱多样性研究 总被引:1,自引:1,他引:0
采用聚丙烯酰胺凝胶电泳技术对木耳(Auriculariaauricula(Hook)Underw)10个栽培菌株酯酶同工酶的酶谱多样性进行了研究。10个供试菌株的幼龄菌丝(7d)中仅检测到25条酶带,各个菌株分别具有2~3条酶带,10个菌株仅有2种酶谱类型;老龄菌丝(72d)中共检测到44条酶带,各个菌株分别具有3~6条酶带,10菌株共有8种酶谱类型。研究表明,木耳双核体菌丝中某些酯酶同工酶基因位点在一定的发育时期才开始表达,老龄菌丝酯酶同工酶酶谱在木耳菌株鉴别和遗传育种研究中具有更大的应用价值。 相似文献
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蜥蜴目同工酶表型效应与目内物种及种群间亲缘关系的研究 总被引:2,自引:0,他引:2
采用聚丙烯酰胺垂直板凝胶电泳技术和特异性的组织化学染色方法对分布于香港的Gekko chinensis、广东南澳岛和四川成都地区的Gekko subpalmatus 2个种群、以及香港和南澳岛的Hemidactylus bowringii 2个种群蜥蜴目动物肝脏、骨骼肌的乳酸脱氢酶 (LDH) 和酯酶 (EST )进行电泳分析。研究结果表明:属内种间具有明显的酶谱差异,每个种都有其特征酶带,据酶谱排序结果可以将5个种群分为2个类群,其中Hemidactylus bowringii的2个种群为一类群,Gekko chinensis和Gekko subpalmatus的2个种群为另一类群。
Studies of the Phenotypic Character and Consanguinity of Isozymes in Species and Population
HAN Ya-li1,XIAO Xiang1,JAMES Lazell2,LU Wen-hua2
1.Department of Biology,Science Institute,Shantou University,Santou,Guangdong Pvovince 515063,China;
2.Department of Herpetology,Museum of Comparative Zoology,Harvard University,Massachusetts 02138,U.S.A.
Abstract:Using discontinuous polyacrylamide gel electrophoresis and histochemistry methods,lactate and esterase isozyme of three species and five popalation of Lacertiformes were analysed.The interspecific zymogramatic differences are obvious.Each species possesses its specific zymogram.According to the zymogramatic similarity among these species calculated with the method of polar ordination,they can be grouped into 2 categories.Gekko chinensis and the two population of Gekko subpalmatus fall into one category.The second category contains the two population of Hemidactylus bowringii.The congsanguinity of the species and popalation has been drawn from the results.
Key words:isozyme; phenotype; Lacertiformes; consanguinity 相似文献
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Esterase isozyme variations ofHeteranthelium piliferum, Taeniatherum asperum andT. crinitum collected in Iraq, Turkey and Iran were analysed by gel isoelectric focusing. InH. piliferum two types of esterase zymogram, H1 and H2, were found. It was demonstrated that a pair of allelic genes, which is shown in the heterozygotes, controls the difference between type H1 and H2. Two types of esterase zymogram, T1 and T2, were observed in two species ofTaeniatherum. The majority of strains having type H1 ofH. piliferum and type T1 ofT. crinitum was distributed in the highly elevated plateau of the Anatolian and Iranian highlands, while strains with H2 and T2 of these two species were found in the western foot-hills of the Zagros Mountains, the Tigris basin of Mesopotamia and the central Anatolian plateau. 相似文献
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Electrophoretic isozyme zymogram patterns of peroxidase (POD), cytochrome oxidase (COD), esterase (Est), α-amylase (α-Amy), catalase (Cat), Glutamate dehydrogenase (GDH), Malate dehydrogenase (MDH), superoxide dismutase (SOD), acid phosphatase (Acp), etc. obtained from 108 maize inbred lines and their 199 hybrids were analyzed. The soluble protein patterns from these materials were tested as well. The authors had probed into the relationship between the index of zymogram difference and potential yield of heterosis in maize. The results indicated that hybrids from parents which showed high zymogram difference index could produce high heterosis and those from parents which had low zymogram difference index could also produce high heterosis as well. But hybrids from the sister lines which had lower zymogram difference index could only produce lower heterosis. There was no significant statistical difference between isozyme zymogram difference index and potential yield of heteroSis on a genetic background with complex combinaton. 相似文献
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滇池高背鲫和方正银鲫酯酶、乳酸脱氢酶同工酶的比较研究 总被引:7,自引:1,他引:6
本研究以方正银鲫(Carassius auratus gibelio Bloch)普通鲫(Carassius auratus)和滇池高背鲫(Carassius sp.)的各种组织器官为材料,进行酯酶(Esterase)和乳酸脱氢酶(LDH)同工酶电泳图谱的分析比较。结果表明:9种不同组织中酯酶同工酶谱带各不相同,有明显的组织特异性。滇池高背鲫的酯酶谱图有3种表型。方正银鲫和滇池高背鲫同一组织的LDH同工酶酶谱也有明显差异。等电聚焦凝胶电泳(T=7.5%,C=5%)的结果又表明这二种鱼的肝脏、脑、卵的酯酶同工酶酶谱及电泳扫描图亦有差异。这些结果揭示滇池高背鲫与方正银鲫至少在生化水平上已有明显的分化,很可能起源于不同的地区,由不同的祖先,独立演化而形成。滇池高背鲫与云南普通鲫的LDH酶谱较为接近,这说明滇池高背鲫最可能起源于云南本地的普通鲫。 相似文献
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Ke-ming Cui Zhong-ming Zhang Ju-huai Li Shao-wen Li Zheng-li Li Chenglee Lee 《Trees - Structure and Function》1995,9(3):165-170
The isozyme zymogram of peroxidase and esterase, and some cell inclusion contents were changed with the differentiation of
regenerated vascular stem tissues after girdling inBroussnetia papyrifera (L.). Vent. Presence or disappearance of some peroxidase and esterase isozyme bands was related to wounding. Some isoperoxidase
bands disappeared at the time of vascular tissue formation, but some esterase isozyme bands appeared in phloem or cambial
regions as sieve-like elements or mature xylem were formed. The inclusion grains progressively disappeared with the formation
of callus and initiation of vascular meristems. The cell inclusions reappeared during the formation of regenrated vascular
tissues. Histochemical study indicated that the inclusion grains could be a complex compound of a protein mass encircling
polysaccharide in the center with a proteinous nucleus. 相似文献
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R. Mancini C. De Pace G. T. Scarascia Mugnozza V. Delre D. Vittori 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1989,77(5):657-667
Summary This study was conducted to assess the genetic basis of the variability observed for the glutamate oxaloacetate transaminase (GOT), Superoxide dismutase (SOD), esterase (EST), and malate dehydrogenase (MDH) isozyme systems in different open-pollinated Vicia faba varieties. Individual plants showing contrasting zymogram patterns were simultaneously selfed and cross-combined. Crossing was unsuccessful in producing progeny, and only selfed progenies were suitable for genetical analysis of isozyme variability. Three zones of GOT activity were made visible. The isozyme of GOT-2 and GOT-3 zones were dimeric and under the control of three alleles at the Got-2 locus and two alleles at the Got-3 locus, respectively. The isozymes of the GOT-1 zone did not show any variability. Three zones of SOD isozyme activity were made visible. The isozymes occurring in the SOD-1 (chloroplastic isozyme form) and SOD-2 (cytosol isozyme form) zones were dimeric and under the control of two alleles at the Sod-1 and Sod-2 loci. The isozyme visualized in the SOD-3 zone (mitochondrial isozyme form) were tetrameric and under the control of two alleles at the Sod-3 locus. Apparently the isozymes made visible in the most anodal esterase zones EST-1, EST-2, and EST-3 were monomeric, and the occurrence of two alleles at each of two different loci explained the variability observed in the EST-2 and EST-3 zones. For MDH, only two five-banded zymogram pattern types were found, and every selfed progeny showed only one of the two zymogram type, indicating that each individual possessed fixed alleles at the loci controlling MDH isozyme. Got-2, Got-3, Sod-1, Sod-2, and Sod-3 appear to be five new isozyme gene markers that can be useful in Vicia faba breeding for linkage study, varietal fingerprinting, outcrossing rate estimate, and indirect selection for quantitative characters. 相似文献
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本文报导了根霉属(Rhizopus) 9个菌株天然态及解聚态可溶性蛋白、酯酶同工酶、葡萄糖淀粉酶和SOD电泳图谱的比较研究。结果表明:可溶性蛋白图谱和酯酶同工酶谱能显示五种已知供试菌种间的差异,尤其酯酶同工酶谱还能显示米根霉两个供试菌株之间的微小差异。经综合分析全部试验结果后得出的系统树图显示了9个供试菌株间的亲缘关系,并为未知菌株F1(BR12)和Q303提供了鉴定和命名依据。文中首次报导了根霉的SOD同工酶,并对蛋白质和酶电泳图谱用于根霉分类研究进行了讨论。 相似文献
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The isozyme make up of esterases of the seeds from fifteen species and twenty-three cultivar of Gossypium was analyzed by isoelectrofocusing. The experimental results are summarized as follows: 1. Differeces were observed in the number of esterase isozyme bands among the species of different genome groups. The cultivated species, G. hirsutum (AD)1 gave rise to 46 isozyme bands, the most among the species of the genus Gossypium. G. barba- dense (AD)2, G. arboreum (A2) and G. herbaceum (A1) gave rise to 42, 40 and 38 bands, respectively. In wild species, G. australe (C3) had 20 esterase bands, the least in all species of Gossypium. The bands given rise from other wild species ranged from 26 to 40. 2. Each species of every genome groups had its marker bands. The results were in agreement with the traditional classification and provided some biochemical evidence for modern classification of Gossypium. 3. It was clear that all cotton species of different genome groups contain 5 main isozyme bands, viz. PI=3.85, 4.61, 5.48, 5.73 and 5.91 in the zymograms. In other words, these zymograms are common characters of Gossypium. 4. The esterase of 23 cultivers in four cultivated species studied showed that no variation in isozyme patterns existed within one species, except the disease-resistant variety Hea-7124 which differs from other 4 cultivars of G. barbadense. 相似文献