Studies on the Chemical Constituents of Rourea microphylla (Hook. et Arn) Planch.

Eleven compounds were isolated from the twigs and leaves of Rourea microphylla (Hook. et Arn) Planch. They were identified as quercetin-3-O-α-L-rhamnopyranoside (Ⅰ), hyperin (Ⅱ), quercetin (Ⅲ), astilbin (Ⅳ), β-sitosterol (Ⅴ), β-sitosteryl-β-D-glucopyranoside (Ⅵ), physcion (Ⅶ), erythroglaucin (Ⅷ), stearic acid (Ⅸ), palmitic acid (Ⅹ) and n-nonacosanane (Ⅺ) respectively, by means of UV, IR, NMR, MS spectra analyses and through chemical methods. The compounds Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅵ, Ⅶ, Ⅷ, Ⅸ, Ⅹ and Ⅺ were first reportedin the Rourea genus.     

Separation and Identification of Chemical Constituents of Dysosma majorense(Gagn.)Hsiao et Y. H. Chen,nov comb

Ten compounds, namely physcion (Ⅰ), podophyllotoxone (Ⅱ), isopicropodophyllone (Ⅲ), picropodophyllone (Ⅳ), 4'-demethyl-podophyllotoxone (Ⅴ), dehydropodo- phyllotoxin (Ⅵ), diphyllin (Ⅶ), picropodophyllin (Ⅷ), podophyllotoxin (Ⅸ) and 4'-demethylpodophyllotoxin (Ⅹ), were separated from Dysosma majorense (Gagn.) Hsiao et Y. H. Chen, nov comb. Compound Ⅳ was first isolated from natural source and anthraquinone Ⅰ was first isolated from genus Dysosma. Ⅴ, Ⅷ and Ⅹ showed strong acttve inhibited against P388.     

The Structure of Parvifolinoic Acid Isolated from Leaves of Isodon parvifolia

Eleven compounds were isolated from the leaves of lsodon parvifolia (Batalin) Hara. On the basis of spectral analysis and chemical methods, crystal Ⅳ was identified as a new compound, named parvifolinoic acid; Crystals Ⅴ–Ⅹ were identified as stearic acid (Ⅴ), β-sito- sterol (Ⅵ), daucosterol (Ⅶ), olenoic acid (Ⅷ), ursoric acid (Ⅸ), 2α-hydroxy-ursoric acid (Ⅹ) respectively. Primary pharmacology tests showed that crystal Ⅳ had inhibitory effect against QGY-7703.     

Studies on the Alkaloids of Stephania succifera Lo. et Tsoong

Eleven alkaloids were isolated from roots of Stephania succifera Lo et Tsoong. They were identified as crebanine (Ⅰ), tetrahydropalmatine (Ⅱ), dehydrocrebanine (Ⅲ), palmatine (Ⅳ). dehydrocorydalmine (Ⅴ), crebanine nitrate (Ⅵ), oxocrebanine (Ⅶ), corydalmine (Ⅷ), phanostenine (Ⅸ), discretamine (Ⅹ) and corypalmine (Ⅺ) by means of TLC. UV, NMR MS spectral analyses and the fact that no depression of MP. with authentic samples was observed. Compounds Ⅳ, Ⅴ, Ⅵ, Ⅶ, Ⅸ, Ⅹ and Ⅺ were first reported from this plant.     

Chemical Constituents in Inula salsoloides (Turcz.) Ostenf.

Nine known compounds, taraxasterol(Ⅰ), taraxasteryl acetate (Ⅱ), taraxasteryl palmitate (Ⅲ), daucosterol (Ⅳ), luteolin(Ⅴ), stigmastanol(Ⅵ), β-sitosterol(Ⅶ), melissic acid(Ⅷ) and eupatolide(Ⅳ), were isolated from lnula salsoloides for the first time. Their structures were determined from spectra date and chemical evidence. (Ⅲ) and (Ⅸ) exhibited cytotoxic effects.     

Studies on the Alkaloids of Stephania yunnanensis

Ten known alkaloids, sinoacutine (Ⅰ), 1-tetrahydropalmatine (Ⅱ), d-stepharine (Ⅲ), 1- stepholidine (Ⅳ), 1-corydalmine (Ⅴ), stephanine (Ⅵ), palmatine (Ⅶ), dehydrocorydalmine (Ⅷ), stepharanine (Ⅸ) and roemerine (Ⅹ) were isolated from the roots of Stephamia yunnanensis H. S. Lo. Their structures were determined from spectral data and chemical evidence.     

银杏中种皮化学成分的分离及鉴定

银杏（Ginkgo biloba Linn．）在中国种植广泛,其产量占全世界总产量的70％[1]。中药白果仁是银杏种子去除肉质外种皮和骨质中种皮后得到的,具有敛肺定喘、止带缩尿的功效。目前,有关银杏种子的研究主要集中在具有药食同源的白果仁[2]及具有潜在应用价值的外种皮[3]上,鲜见对银杏种子中种皮的研究报道。生产中大量的银杏中种皮被废弃,因此,开展银杏中种皮的相关研究对于充分利用该类资源非常必要。鉴于此,作者对银杏中种皮的化学成分进行了系统分析,以期为其开发应用提供参考依据。     

The Chemical Constituents of Antitumor from Sabina vulgaris Ant

Eleven known compounds, transcommunic acid (Ⅰ), cis-communic acid (Ⅱ), sandaracopimaric acid (Ⅲ), isocupressic acid (Ⅳ), sugiol (Ⅴ), 12-hydroxy-6, 7-seco- abieta-8, 11, 13-triene 6, 7-dial (Ⅵ), β-peltatin-A-methyl ether (Ⅶ), bergaptin (Ⅷ), β-sitosterol (Ⅹ), lignoceric acid (Ⅺ), cerotic acid(Ⅻ) and a new 3, 4-benzocumarins sabilactone (Ⅸ) were newly isolated from the bark of Sabina vulga ris Ant. (= Juniperus sabina L.). Their structures were determined from spectra data and chemical evidence. Compounds Ⅳ–Ⅹ were first isolated from this plant. The bioassay results showed that compounds Ⅰ–Ⅳ, Ⅵ andⅦ were shown to be cytotoxic effects for P-388 leukemia cell in vitro.     

The Chemical Constituents from Cynanchum Komarovii Al. Ilinski

Ten compounds were isolated from the roots and stems of Cynanchum komarovii Al. Iljinski. They were identified as 7-demethoxylophorine (Ⅰ), epi-alnusenol (Ⅱ), β-sitosterol (Ⅲ), triacontanolic acid (Ⅳ), sucrose (Ⅴ), desoxytylophorinin N-oxide (Ⅵ), apocynine (Ⅶ), paeonol (Ⅷ), β-sitosteryl-β-D-glucopyranoside (Ⅸ) and glucose (Ⅹ) by means of UV, IR, NMR, MS spectral analyses and through chemical methods. These compounds were first reported in this plant. One of them, Ⅵ is a new alkaloid. The bioassay results showed that the compounds Ⅰ, Ⅵ and reaction product Ⅺ exhibited cytotoxic effects to P-388 leukemia cell in vitro. Ⅺ is also a new compound.     

小叶黄杨化学成分的研究

小叶黄杨氯仿组分通过硅胶柱色谱分离纯化,从中分离得到9个化合物,分别鉴定为:β-谷甾醇(β-Sitosterol,Ⅰ)、豆甾醇(Stigmasterol,Ⅱ)、胡萝卜甙(daucosterol,Ⅲ)、水杨酸(salicylis acid,Ⅳ)、香草酸(vanillicacid,Ⅴ)、5,4′-二羟基-3,3′,7-三甲氧基-黄酮(5,4′-dihydroxy-3,3′,7-trimethoxy-flavone,Ⅵ)、5,4′-二羟基-3,3′,6,7-四甲氧基-黄酮(5,4′-dihydroxy-3,3′,6,7-tetramethoxy-flavone,Ⅶ)、Cleomiscosin A(Ⅷ)、3,5-二羟基-4′,6,7-三甲氧基-黄酮-3′-O-β-D-葡萄糖甙(3,5-dihydroxl-4′,6,7-trimethoxyl-flavone-3′-O-β-D-glucopyranoside,Ⅸ),其中化合物Ⅳ、Ⅴ、Ⅵ、Ⅶ均首次从该属植物中分离得到。     

The Chemical Constituents of Elsholtzia ciliata (Thund.) Hyland

Fourteen compounds have been obtained from the Elsholtzia ciliata (Thund.) Hyland. Their structures were identified by spectral and chemical methods as following: Ⅰ. 6-methyl-tritriacontane, Ⅱ. 13-cyclohexyl-hexoacosane, Ⅲ. β-sitosterol, Ⅳa. palmitic acid, Ⅳb. linoleic acid, Ⅳc. linolenic acid, Ⅴ. ursolic acid, Ⅵ. 5-hydroxy--6, 7-dimethoxyflavone, Ⅶ. 5-hyd-foxy-7, 8-dimethoxyflavone, Ⅷ. 5,7-dihydroxy-4'-methoxyflavone, Ⅸ. 5-hydroxy-7,4'-dime-thoxyflavone, Ⅹ.β-sitosterol-β-D-glucoside, Ⅺ. 5-hydroxy-6-methylflavanone-7-O-α-D-galacopy-ranoside, Ⅻ. acacetin-7-O-β-glucoside. Among all the compounds, Ⅺ is a new compound. Except for Ⅳb and Ⅳc, the other twelve compounds were first found in this plant.     

Chemical Constituents of Glycyrrhiza eurycarpa P. C. Li (1I)

Huang Gancao (Glycyrrhiza eurycarpa P. C. Li) is a new species discovered recently. Its chemical constituents have not been reported before. This paper reports the isolation of ten components (Ⅰ—Ⅹ) from its roots and rhizomes collected in Jinta County, Gansu Province. On the basis of physico-chemlcal constants and spectroscopic (IR, UV, 1HNMR, 13CNMR, MS, and CD) analysis, they were identified as ononin(Ⅰ), liquiritigenin 4 -O-β-D-apio-D-franosyl-(1 →2)-β-D-glucopyranoside(II), isoliquiritigenin-4-O-β-D-apio-D-franosyl-(1→ 2) β-D-glucopyranoside (Ⅲ), choerospondin (Ⅳ), violanthin (Ⅴ), isoviolan thin (Ⅵ), vicenin-2(Ⅶ), uralsaponin A(Ⅷ), daucosterol (Ⅸ), and phloretic acid (Ⅹ), respectively All of them were isolated from this plant for the first time. Choerospondin, violanthin, daucosteroi and phloretic acid were not found in this genus before.     

Biotransformation of 3b-Hydroxyandrost-5-en-17-one by Cell Suspension Cultures of Catharanthus roseus

Catharanthus roseus (L.) G. Don cell suspension cultures were used to transform 3b-hydroxyandrost-5-en-17-one, the products were isolated by chromatographic methods. Their structures were established by means of NMR and MS spectral analyses. Nine metabolites were respectively elucidated as: androst-4-ene-3,17-dione (Ⅰ), 6a-hydroxyandrost-4-ene-3,17-dione (Ⅱ), 6a,17b-dihydroxyandrost-4-en-3-one (Ⅲ), 6b-hydroxyandrost-4-ene-3,17-dione (Ⅳ), 17b-hydroxyandrost-4-en-3-one (Ⅴ), 15a,17b-dihydroxyandrost-4-en-3-one (Ⅵ), 15b,17b-dihydroxyandrost-4-en-3-one (Ⅶ), 14a-hydroxyandrost-4-ene-3,17-dione (Ⅷ), 17b-hydroxyandrost-4-ene-3,16-dione (Ⅸ). It is the first time to obtain the above compounds by biotransformation with Catharanthus roseus cell cultures.     

Study on Chemical Constituents of Gentianopsis barbata var. Stennocalyx H. W. Li ex T.N. Ho

Nine compounds were isolated from Gentianopsis barbata var. stennocalyx H. W. Li ex. T.N.Ho. Their structures are identified as 1-hydroxy4, 7, 8-trimethoxyxanthone (Ⅰ), 1, 7-dihydroxy-3, 8-dimethoxyxanthone (Ⅱ), 1, 7, 8-trihydroxy-3-methoxyxanthone (Ⅲ), 1-O-(β-D- xylopyranosyl-(1→6)-β-D-glucopyranosyl)-3, 7, 8-trimethoxyxanthone (Ⅳ), 1-O-(β-D-xylopy- ranosyl- (1→6)-β-D-glucopyranosyl)-7-hydroxy-3, 8-dimethoxyxanthone (Ⅴ), 1-O-(β-D-xylo- pyranosyl-(1→6)-β-D-glucopyranosyl)-7, 8-dihydroxy-3-methoxyxanthone (Ⅵ), luteolin-7-O- β-D-glucoside (Ⅶ), oleanolic acid (Ⅷ) and ursolic acid (Ⅸ) by means of chemical methods and UV, IR and NMR determinations respectively.     

Study on the Chemical Constituents of Comastoma pulmonarium (Turcz.) Toyohuni

Eight compounds were isolated from Comastoma pulmonarium (Gentianaceae). Theirstructures are identified as1,8-dihydroxy-3,5-dimethoxyxanthone(Ⅰ),1,8-dihydroxy-3,7-dimetho-xyxanthone(Ⅱ), 1-hydroxy-3,7,8-trimethoxyxanthone(Ⅲ),8-hydroxy-l,3,5-trimethoxyxanthone(Ⅳ), 1,3,8-trihydroxy-7-methoxyxanthone(Ⅴ), 1-O-β-D-glucopyranosyl-3,8-dihydroxy-7-methxy-xanthone(Ⅵ), oleanolic acid(Ⅶ) and swertisin(Ⅷ) by means of chemical methods andUV, IR, NMR and MS respectively. The compound Ⅵ is a new natural product. It wasnamed as comastomaside.     

短尾越橘化学成分研究

短尾越橘酒精提取物用乙酸乙酯萃取后依次经氯仿、丙酮、甲醇回流提取,用薄层层析,反复硅胶柱层析,从中分离并鉴定了八个化合物,并用波谱学方法进行了结构鉴定。它们分别为正二十七烷(n-heptaco-sane,Ⅰ),木栓酮(friedelin,Ⅱ),木栓醇(friedelinol,Ⅲ),羽扇豆醇(lupenol,Ⅳ),β-谷甾醇(β-sitosterol,Ⅴ),胡萝卜苷(dauosterol,Ⅵ),反式对羟基桂皮酸(trans-p-hydroxy cinamic acid,Ⅶ),莽草酸甲酯(methyl shiki mate,Ⅷ)。所有化合物均为首次从该植物中分离得到,化合物Ⅰ,Ⅳ,Ⅷ为首次从该属植物中得到。     

Discovery, identification and comparative analysis of non-specific lipid transfer protein (nsLtp) family in Solanaceae

Plant non-specific lipid transfer proteins(nsLtps) have been reported to be involved in plant defense activity against bacterial and fungal pathogens.In this study,we identified 135 (122 putative and 13 previously identified) Solanaceae nsLtps,which are clustered into 8 different groups.By comparing with Boutrot's nsLtp classification,we classified these eight groups into five types (Ⅰ,Ⅱ,Ⅳ,Ⅸ and Ⅹ).We compared Solanaceae nsLtps with Arabidopsis and Gramineae nsLtps and found that (1) Types Ⅰ,Ⅱ and Ⅳ are shared by Solanaceae,Gramineae and Arabidopsis;(2) Types Ⅲ,Ⅴ,Ⅵ and Ⅷ are shared by Gramineae and Arabidopsis but not detected in Solanaceae so far;(3) Type Ⅶ is only found in Gramineae whereas type Ⅸ is present only in Arabidopsis and Solanaceae;(4) Type X is a new type that accounts for 52.59% Solanaceae nsLtps in our data,and has not been reported in any other plant so far.We further built and compared the three-dimensional structures of the eight groups,and found that the major functional diversification within the nsLtp family could be predated to the monocot/dicot divergence,and many gene duplications and sequence variations had happened in the nsLtp family after the monocot/dicot divergence,especially in Solanaceae.     

The Xanthones of Swertia erythtrosticta Maxim

Swertia erythrosticta Maxim. (Gentianaceae) is a herb used for curing some diseases in Tibetan traditional medicine system. Eight xanthones were isolated from this species. Their structures were identified as 1,8-dihydroxy-3,7-dimethoxyxanthone(Ⅰ), 1,5,8-trihydroxy-3-methoxyxanthone (Ⅱ), 1,3,8-trihydroxy-5-methoxyxanthone (Ⅲ), 1,7,8-trihydroxy-3-methoxyxanthone (Ⅳ), 1,3,5,8-tetrahydroxyxanthone (Ⅴ), 1,3,7,8-tetrahydroxyxanthone(Ⅵ), as well as 8-O-β-D-glucopyranosyl-1, 5-dihydroxy-3 -metho xy xanthone(Ⅶ), 8-O-β-D-gl ucopyr anosyl- 1,3,5- trihydroxyxanthone(Ⅷ) by means of chemical methodes and spectral analyses.     

Studies on the Constituents from Marsdenia globifera

Two novel C-21 steroidal aglycones named marsglogenin-A(Ⅱ) and marsglogenin-B(Ⅲ) as well as six known compounds sarcostin (Ⅰ), gymnemarsgenin (Ⅳ), 20-O-cinnamoylsarcostin (Ⅴ), penupogenin (Ⅵ), gagaminin (Ⅶ) and isolineolcn (Ⅷ) were isolated from the acid hydrolysate of the crude glycoside extracted with ethanol from the stem of Marsdenia globitera (Asclepiadaceae). The structures of marsglogenin-A and marsglogenin-B were deduced as 20-O-benzoylsarcostin and 12-O-benzoylsarcostin on the bases of chemical and spectral evidences respectively.     

Studies on the Flavonoids from the Pollens of Typha davidiana,T. Latifolia and T. angustata(Pu Huang)

Eight identical flavonoids were isolated from the alcoholic extract of the pollen of Typha davidiana Hand.-Mazz., Typha latifolia L. and Typha angustata Bory et Chaub. On the basis of spectroscopic (IR, UV, MS and NMR) analysis and the identification of the acidic hydrolytic products, they were identified as naringenin (Ⅰ), isorhamnetin (Ⅱ), quercetin (Ⅲ), isorhamnetin-3-O-(2G-α-L-rhamnopyrano-syl)-rutinoside(Ⅳ), quercetin-3-O-(2G-α-L-rhamnopyranosyl)-rutinoside (Ⅴ), iso-rhamnetin-3-O-rutinoside (Ⅵ),isorhamnetin-3-O-neohesperidoside (Ⅶ), kampferol-3-O-neohesperidoside (Ⅷ). Compound Ⅴ was found in this genus for the first time.