金鱼藻的化学成分

从金鱼藻(Ceratophyllum demersum L.)全草的乙醇提取物中分离鉴定了其中7个,分别为:苜蓿素-7-O-β-D-葡萄糖苷(1) , naringenin-7-O-β-D-葡萄糖苷(2) ,七叶内酯(3) ,β-谷甾醇(4) , 7α-羟基-β-谷甾醇(5) , 7α-甲氧基-β-谷甾醇(6) ,十六碳脂肪酸(7)。化合物(3)为首次从金鱼藻中分离得到。     

中国繁缕属的一些分类问题

研究了36种中国产繁缕属(Stellaria)植物,根据模式材料或仅根据原描述,将已合格发表的1组、l系、12种、4变种和1变型首次归并到有关各类,依次列举人异名录中,其中有1移属。讨论了各种错误鉴定,同时对于选择性状、设想进化过程和分布记录均有讨论.     

金鱼藻的化学成分

从金鱼藻( Ceratophyllum demersum L .) 全草的乙醇提取物中分离鉴定了其中7 个, 分别为: 苜蓿素-7- O-β- D-葡萄糖苷( 1) , naringenin-7- O-β- D-葡萄糖苷(2 ) , 七叶内酯(3) , β-谷甾醇(4 ) , 7α-羟基-β-谷甾醇(5) , 7α-甲氧基-β-谷甾醇(6) , 十六碳脂肪酸(7) 。化合物(3) 为首次从金鱼藻中分离得到。     

Non-glycosidic iridoids from Cymbaria mongolica

Six non-glycosidic iridoids, i.e. (1R,4S,4aS,7S,7aS)-7-hydroxyl-4-hydroxy- methyl-7-methyl-1-methoxyl-1,4,4a,7a-tetrahydrocyclopenta[e]pyran-3-one (1), (1S,4R,4aS,7S,7aS)-7-hydroxyl-4-hydroxymethyl-7-methyl-1-methoxyl-1,4,4a,7a-tetrahydrocyclopenta[e]pyran-3-one (2), (1R,4R,4aS,7S,7aS)-7-hydroxyl-4-hydroxy-methyl-7-methyl-1-methoxyl-1,4,4a,7a-tetrahydrocyclopenta[e]pyran-3-one (3), (1R, 4R, 4aS, 7aS)-4,7-dihydroxymethyl-1-methoxyl-1,4,4a,7a-tetrahydrocyclopenta-6-ene[e]pyran-3-one (4), (1R, 4R, 4aS, 7aS)-4,7-dihydroxymethyl-1-hydroxyl-1,4,4a, 7a-tetrahydrocyclopenta-6-ene[e]pyran-3-one (5), (1R, 4S, 4aS, 7aS)-4,7-dihydroxy-methyl-1-methoxyl-1,4,4a,7a-tetrahydrocyclopenta-6-ene[e]pyran-3-one (6), as well as five known non-glycosidic iridoids mussaenin A (7), gardendiol (8), isoboonein (9), 4-epi-alyxialactone (10) and rehmaglutin D (11) have been isolated from the Chinese medicinal plant Cymbaria mongolica. Their structures were elucidated by spectroscopic methods. These compounds exhibit significant antitumor and antibacterial activity.     

毛脉酸模根中一新的色原酮苷类化合物

从毛脉酸模根(Rumex gmelini Turcz.)75%乙醇提取物中分离得到1个新色原酮苷和5个已知化合物,应用波谱学方法及文献对照分别鉴定为2,5-dimethyl-7-hydroxychromone-7-O-β-glucopyranoside (1),nepodin-8-O-β-D-glucopyranoside (2),10-hydroxyaloin A (3),10-hydroxyaloin B (4),5-methoxyl-1(3H)-benzofuranone-7-O-β-D-glucopyranoside (5),phenylethyl-O-α-L-arabinopyranosy-(1→6)-O-β-D-glucopyranoside (6). 其中化合物1为新化合物,3～6首次从酸模属中分离得到,化合物2首次从该植物中分离得到.     

旋花茄新鲜果实中的三个甾体皂苷

从茄科食用植物旋花茄(Solanum spirde)的新鲜果实中分离得到3个化合物,其中一个为新成分,经现代波谱学方法鉴定为26-0-β-D-葡萄吡喃糖基-(25R)-呋甾-3β,22ξ,26-三醇-5-烯-3-0-α-L-鼠李吡喃糖基-(1-2)-[3-0-(3-O-乙酰基)-α-L-鼠李吡喃糖基-(1-4)]-β-D-葡萄吡喃糖苷(1).2个已知化合物分别为26-O-β-D-葡萄吡喃糖基-(25R)-呋甾-22ξ-甲氧基-3β,26-二醇-5-烯-3-O-α-L-鼠李吡哺糖基-(1-2)-β-D-葡萄吡喃糖苷(2)和26-O-β-D-葡萄吡喃糖基-(25R)-呋甾-3β,22ξ,26-三醇-5-烯-3-O-α-L-鼠李吡喃糖基-(1-2)-[α-L-鼠李吡喃糖基-(1-4)]-β-D-葡萄吡喃糖苷(protodioscin)(3),均为首次从该植物中分离得到.     

Steroidal Alkaloids from Sarcococca vagans

Sarcococca vagans Stapf (Buxaceae) was distributed in Southern Yunnan, China. From the plant collected in Xishuangbanna,three new steroidal alkaloids named vaganines A,B and C( Ⅰ–Ⅲ )together with three known compounds were isolated and on the basis of spectral analyses and chemical evidence, the chemical structures of three new compounds were determined as 20α-dimethylamino-4β-acetoxyl-3β-senecioylamino-5α-pregnane (Ⅰ), 20α-dimethylamino-4β hydroxyl- 3-senecioylamino-△2(3)-pregnaene (Ⅱ), acetate of Ⅱ (Ⅲ); respectively.     

Aldo-keto reductases AKR1C1, AKR1C2 and AKR1C3 may enhance progesterone metabolism in ovarian endometriosis

Endometriosis is a very common disease that is characterized by increased formation of estradiol and disturbed progesterone action. This latter is usually explained by a lack of progesterone receptor B (PR-B) expression, while the role of pre-receptor metabolism of progesterone is not yet fully understood. In normal endometrium, progesterone is metabolized by reductive 20α-hydroxysteroid dehydrogenases (20α-HSDs), 3α/β-HSDs and 5α/β-reductases. The aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) are the major reductive 20α-HSDs, while the oxidative reaction is catalyzed by 17β-HSD type 2 (HSD17B2). Also, 3α-HSD and 3β-HSD activities have been associated with the AKR1C isozymes. Additionally, 5α-reductase types 1 and 2 (SRD5A1, SRD5A2) and 5β-reductase (AKR1D1) are responsible for the formation of 5α- and 5β-reduced pregnanes. In this study, we examined the expression of PR-AB and the progesterone metabolizing enzymes in 31 specimens of ovarian endometriosis and 28 specimens of normal endometrium. Real-time PCR analysis revealed significantly decreased mRNA levels of PR-AB, HSD17B2 and SRD5A2, significantly increased mRNA levels of AKR1C1, AKR1C2, AKR1C3 and SRD5A1, and negligible mRNA levels of AKR1D1. Immunohistochemistry staining of endometriotic tissue compared to control endometrium showed significantly lower PR-B levels in epithelial cells and no significant differences in stromal cells, there were no significant differences in the expression of AKR1C3 and significantly higher AKR1C2 levels were seen only in stromal cells. Our expression analysis data at the mRNA level and partially at the cellular level thus suggest enhanced metabolism of progesterone by SRD5A1 and the 20α-HSD and 3α/β-HSD activities of AKR1C1, AKR1C2 and AKR1C3.     

Novel taxa-4(20),12-diene and 2(3→20)abeotaxane from needles of Taxus canadensis

A novel 6/8/6-membered taxane with a rare C-12(13)-double bond and rare 2(3→20)abeotaxane were isolated from the needles of Taxus canadensis. Their structures were characterized as 7β,9α,10β-triacetoxytaxa-4(20),12-diene-2α,5α,11β-triol (1) and 2α,7β,10β-triacetoxy-5α-hydroxy-2(3→20)abeotaxa-4(20),11-diene-9,13-dione (2) on the basis of 1D and 2D spectroscopic data. 1 is the first example of a natural taxane without substitution at both C-13 and C-14.     

Cytotoxic terpenoids from Juglans sinensis leaves and twigs

Bioassay-guided fractionation of an 80% MeOH extract of Juglan sinensis leaves and twigs has resulted in the isolation of three new triterpenes (1-3) and two new sesquiterpenes (4-5) along with two known sesquiterpenes (6-7). The new compounds were determined to be 3β, 11α, 19α, 24, 30-pentahydroxy-20β, 28-epoxy-28β-methoxy-ursane (1), 1α, 3β-dihydroxy-olean-18-ene (2), 2α, 3α, 23-trihydroxy-urs-12-en-28-oic acid 28-O-β-d-glucopyranoside (3), (4S, 5S, 7R, 8R, 14R)-8, 11-dihydroxy-2, 4-cyclo-eudesmane (4), 15-hydroxy-α-eudesmol-11-O-β-d-glucopyranoside (5), by spectroscopic analysis. The cytotoxicity of compounds (1-7) against four cancer cell lines such as B16F10, Hep-2, MCF-7 and U87-MG was evaluated. Compounds 1, 2, 6 and 7 showed potent cytotoxicity against all of four cancer cell lines, respectively.     

Pendulaosides A and B. Two acylated triterpenoid saponins from Harpullia pendula seed extract

Two new acylated triterpenoid saponins named pendulaosides A and B as well as the known phenolic compounds methyl gallate, gallic acid, 1,2,3,6-tera-O-galloyl-β-d-glucose and 1,2,3,4,6-penta-O-galloyl-β-d-glucose, were isolated from the seeds of Harpullia pendula. The structures of pendulaosides A and B were determined using extensive 1D and 2D NMR analysis and mass spectrometry as well as acid hydrolysis, as 3-O-β-d-glucopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→3)]-β-d-glucuronopyranosyl-22-O-angeloyl-3β,16α,22α,24β,28-pentahydroxylolean-12-ene and 3-O-β-d-glucopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→3)]-β-d-glucuronopyranosyl-16-O-(2-methylbutyroyl)-3β,16α,22α,24β,28-pentahydroxylolean-12-ene, respectively. To the best of our knowledge the two triterpene parts 22-O-angeloyl-3β,16α,22α,24β,28-pentahydroxylolean-12-ene and16-O-(2-methylbutyroyl)-3β,16α,22α,24β,28-pentahydroxylolean-12-ene have never been characterized before. The two isolated saponins were assayed for their in-vitro cytotoxic activity against the three human tumor cell lines HepG2, MCF7 and PC3. The results showed that pendulaoside A exhibited moderate activity on PC3 cell line with IC50value equal to 13.0 μM and weak activity on HepG2 cell line with IC50 value equal to 41.0 μM. Pendulaoside B proved to be inactive against the three used cell lines.     

中华小苦荬萜类化学成分的研究(英文)

从中华小苦荬全草的乙酸乙酯提取物中分离得到8个萜类化合物,通过波谱方法及文献对照分别鉴定为β-香树脂素(1),3β-羟基-20(30)-蒲公英甾烯(2),熊果-12-烯-3β-醇(3),羽扇豆醇(4),10-羟基艾里莫芬-7(11)-烯-12,8α-内酯(5),乌苏-12,20(30)-二烯-3β,28-二醇(6),3β,8α-二羟基-6β-当归酰基艾里莫芬-7(11)-烯-12,8β-内酯(7)和乌苏酸(8),化合物1～8均首次从该植物中分离得到。     

Steroidal saponins and cytoxicity of the wild edible vegetable-Smilacina atropurpurea

Four new steroidal saponins, smilacinoside A (1), B (2), C (3), and D (4), together with three known saponins, funkioside D (5), aspidistrin (6) and 26-O-beta-d-glucopyranosyl-22-methoxyl-(25R)-furost-5-en-3beta,26-diol 3-O-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranosyl-(1-->4)-beta-d-galactopyranoside (7) were isolated from the dried tender aerial parts of Smilacina atropurpurea (Franch.) Wang et Tang. The structures of new compounds were elucidated as diosgenin 3-O-alpha-l-rhamnopyranosyl-(1-->2)-O-beta-d-galactopyranoside (1), diosgenin 3-O-alpha-l-rhamnopyranosyl-(1-->3)-[6-O-palmitoxyl]-O-beta-d-galactopyranoside (2), 26-O-beta-d-glucopyranosyl-(25R)-furost-5-en-3beta,22xi,26-triol 3-O-alpha-l-rhamnopyranosyl-(1-->2)}-beta-d-galactopyranoside (3) and 26-O-beta-d-glucopyranosyl-22-methoxyl-(25R)-furost-5-en-3beta,26-diol 3-O-alpha-l-rhamnopyranosyl-(1-->2)-beta-d-galactopyranoside (4) on the basis of chemical methods and detailed spectroscopic analysis, including 1D and 2D NMR techniques and single-crystal X-ray diffraction, respectively. Six of these compounds and MeOH extract were tested for their in vitro cytotoxicity toward K562 human tumor cells by an improved MTT method. Smilacinoside A, funkioside D and aspidistrin exhibited significant in vitro cytotoxicity against K562 with IC(50) values of 1.09, 2.93 and 0.47microg/mL, respectively.     

Three New Steroidal Alkaloids from Pachysandra axillaris

This paper describes the chemical structure elucidation of the three new alkaloids isolated from Pachysandra axillarls Franch. They are pachysamine G (20α-dimethylamino-3β-tigloylamino-5α-pregnane), pachysamine H (20α-dimethylamino-3α-N-methyl, benzoylamino-5α-pregnane) and pachysanaximine A (20 α-dimethylamino-3β-methylamino-4β-benzoxyl-5α-pregnane) respectively. Other two compounds were identified as Pachysamine A, B.     

Two new steroidal saponins from Dioscorea panthaica

Two new furostanol saponins, 3-O-[α-l-rhamnopyranosyl-(1→4)-β-d-glucopyranosyl]-26-O-β-d-glucopyranosyl-25(R)-furosta-5,22(23)-dien-3β,20α,26-triol (1), 3-O-[β-d-glucopyranosyl-(1→3)-O-α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranosyl]-26-O-β-d-glucopyranosyl-20(R)-methoxyl-25(R)-furosta-5,22(23)-dien-3β,26-diol (2) were isolated from the Dioscorea panthaica along with five known steroidal saponins (3–7). The structures of the new saponins were determined by detailed analysis of spectral data (including 2D NMR spectroscopy). The inhibitory activities of the saponins against α-glucosidase were investigated, gracillin (4) and 3-O-[α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranosyl]-26-O-β-d-glucopyranosyl-25(R)-furosta-5,20(22)-dien-3β,26-diol (5) were found to exhibit potent activities with IC₅₀ values of 0.11 ± 0.04 mM and 0.09 ± 0.01 mM.     

The Glyxosides from Curculigo orchiodes

Seven compounds have been isolaed from the roots of Curculigo orchioides. On the basis of spectral analysis,their structures were elucidated as 2,6-dimethoxy benzoic acid (A),orcinol glucopyranoside(B),curculigine A(C),curculigoside(D),24s,3β,11α,16,24-tetrahyaroxycycloartenol-3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(E),24s,3β,11a,16β,24-tetrahydroxycycloartenol-3-O-β-d-glucopyranosyl(1→2)-β-D-glucopyranoside(F) and dancosterol(G). Saponin F is a new tritepenoid compound.     

齿棱茎小蒜-中国新记录种

Allium inutile Makino in Bot.Mag.Tokyo 12：104.1898．- Nothoscordum inutile(Makino)kitamura in Bot.Mag.Tokyo 59：35.1946．     

Novel Taxa-4(20),12-diene and 2(3→20)Abeotaxane from Needles of Taxus canadensis

A novel 6/8/6-membered taxane with a rare C-12(13)-double bond and rare 2(3→20)abeotaxane were isolated from the needles of Taxus canadensis. Their structures were characterized as 7β,9α,10β-triacetoxytaxa-4(20),12-diene-2α,5α,11β-triol (1) and 2α,7β,10β-triacetoxy-5α-hydroxy-2(3→20)abeotaxa-4(20),11-diene-9,13-dione (2) on the basis of 1D and 2D spectroscopic data. 1 is the first example of a natural taxane without substitution at both C-13 and C-14.     

Studies of Songbeinone,A New Fritillaria Alkaloid Identified from the Bulb of Fritillaria unibracteata

A compound C was isolated from the dry bulbs of Fritillaria unibracteata Hsiao et K. C: Hsia which belongs to a main resource species of traditional Chinese drug “Chuan Bei-mu” in Chinese Pharmacopoeia (1990 ed.). It was identified as D/E cis(22R, 25S)-20-deoxy-3β-hydroxyl-6-carbonyl-5α, 14α, 17β-cevanine by means of spectroscopic methods. It is a new alkaloid of 5α-cevanine group of isosteroidal alkaloids being attributed to the characteristic constituents of this genus, named as songbeinone.     

Iridoid glucosides and a C₁₃-norisoprenoid from Lamiophlomis rotata and their effects on NF-κB activation

Four new iridoid glucosides, 7-dehydroxyzaluzioside (1), 6'-O-syringylphlorigidoside C (2), barlerin-6″-hydroxy-2″,6″-dimethylocta-2″,7″-dienate ester (3), 6β-n-butoxy-7,8-dehydropenstemonoside (4), and a new C(13)-norisoprenoid derivative, 5β,6α-dihydroxy-3β-(β-D-glucoyranosyloxy)-7-megastigmen-9-one (5), together with 16 known iridoid glucosides, were isolated from Lamiophlomis rotata. The structures of these new compounds were elucidated by HRMS, 1D and 2D NMR spectroscopy. A stable nuclear factor kappaB (NF-κB)-luciferase-expressing human embryonic kidney 293 cell line was used in the luciferase assay for monitoring the anti-inflammatory activity of the compounds. It was found that 6β-n-butoxy-7,8-dehydropenstemonoside (4) and two known compounds (8-epi-7-deoxyloganin and 7,8-dehydropenstemonoside) had a significant inhibitory effect on lipopolysaccharide-stimulated NF-κB activation.