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1.
裂叶悬钩子器官发生的细胞组织学观察 总被引:1,自引:0,他引:1
裂叶悬钩子(Rubus laciniatus W.cv.Thornless Evergreen)是蔷薇科悬钩子属一种重要的小果类植物。我们在以离体叶片诱导不定芽成功的基础上,对其叶片不定芽形态发生过程作了进一步观察。供接种的叶片从基部去掉叶柄,按前文的操作顺序进行灭菌,接种和培养。从培养第3天起,从叶片基部与叶柄接合处切取材料固定,以后每隔2天取样一次,一直取到20 相似文献
2.
本文首次报道裂叶悬钩子(Rubus laciniatus Wild)叶外植体培养在改良的NN~(69)培养基上附加2—4mg/1 6-BA和0.1mg/1 NAA或1—3mg/1 2,4-D和0.1mg/1 NAA,两者都可直接从完整叶片、叶片下切段或叶柄诱导出不定芽。诱导频率达20—48%。而不定芽绝大部分发生在叶轴处或叶柄基部。完整叶片的不定芽诱导率与叶片下切段无差别,但比叶柄基部诱导率要高。6-BA对叶轴处不定芽诱导率比2,4-D的要高。此外,不需继代培养,不定芽数可达10—20个,继代培养一个月左右,每个不定芽能形成丛生芽数可达40一60个。另外,本文还讨论了细胞分化过程中的极性现象。 相似文献
3.
本文首次报道裂叶悬钩子(Rubus laciniatus Wild)叶外植体培养在改良的NN69培养基上附加2—4mg/1 6-BA和0.1mg/1 NAA或1—3mg/1 2,4-D和0.1mg/1 NAA,两者都可直接从完整叶片、叶片下切段或叶柄诱导出不定芽。诱导频率达20—48%。而不定芽绝大部分发生在叶轴处或叶柄基部。完整叶片的不定芽诱导率与叶片下切段无差别,但比叶柄基部诱导率要高。6-BA对叶轴处不定芽诱导率比2,4-D的要高。此外,不需继代培养,不定芽数可达10—20个,继代培养一个月左右,每个不定芽能形成丛生芽数可达40一60个。另外,本文还讨论了细胞分化过程中的极性现象。 相似文献
4.
Hypocotyl protoplasts from oil rape, Brassica napus L. cv. Isuzu were cultured in the dark at 25°C in a modified Nitsch and Nitsch medium containing 13% sucrose, 5 g/l Ficoll, 0.5 mg/l BAP, 1 mg/l NAA and 0.5 mg/l 2–4 D. Protoplasts floated on the surface of the medium and developed into microcolonies 0.5 mm in diameter in 4–6 weeks. The microcolonies also remained on the surface of the medium. Transfer to MS medium supplemented with 200 mg/l casein hydrolysate, 5mg/l BAP, 0.5 mg/l NAA and solidified with 0.6% agarose induced shoot regeneration in 3–4 weeks.Abbreviations BAP
6-benzylaminopurine
- NAA
1-naphthaleneacetic acid
- 2,4-D
2,4 — dichlorophenoxyacetic acid 相似文献
5.
Protoplasts isolated from hypocotyls of three-day-old seedlings of Brassica carinata (Braun) cv R-2128 were cultured in a modified Nitsch and Nitsch liquid medium containing 13% sucrose, 0.4% Ficoll, 0.25 mg/l BA, 0.5 mg/l NAA and 0.5 mg/l 2,4-D. The density of medium caused the protoplasts and the developing microcalli to float on the surface of the liquid medium whereas all debris and lysed cells sank to the bottom of the culture plate. After 4–6 weeks developing microcalli were approximately 0.5 mm in diameter and were transferred onto MS medium containing 3% sucrose, 0.4% agarose, 200 mg/l casein hydrolysate, 5 mg/l BA and 0.5 mg/l NAA, pH 5.7. Approximately 20% of the calli transferred to this medium produced plantlets.Abbreviations BA
6-Benzylaminopurine
- NAA
1-naphthaleneacetic acid
- IAA
indole-3-acetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- MS
Murashiqe-Skoog 相似文献
6.
We investigated the optimal levels of growth regulators, culture media, and pH on callus growth and organogenesis of in-vitro
cultured ‘Kyoho’ grapes. Calli were induced by culturing leaf blades on an MS basal medium supplemented with 1 mg/IL BA and
0.01 mg/L 2,4-D. In addition, calli originating from the exocarp and mesocarp of grape fruits devel-oped on MS media supplemented
with 0.1 mg/L IAA, NAA, or 2,4-D, or with 0.2 mg/L BA. In testing the potential for plant regeneration from shoot tips on
various media, we found that the Nitsch medium, with I mg/L BA, was optimal for caulogenesis. The type of shoot development
depended on the pH of the medium, with vigorous multiple-shoot devel-opment occurring at pH 6.0, and single shoots forming
at pH 5.0. Finally, we were able to obtain rooted seedlings from the regenerated shoots that had been cultured on 1/4-strength
Nitsch medium supplemented with 0.03 mg/L NAA. 相似文献
7.
以唐菖蒲花色突变株开花后的子房为外植体,接种于MS+1.0mg/L BA+0.01-0.1mg/L NAA或MS+1.0mg/LBA+1.0mg/L KT+0.01-0.1mg/LNAA的培养基中,从膨大的组织表面直接诱导不定芽.膨大的组织或带不定芽的组织每隔周转入1/2MS_1.0mg/L BA+0.01-0.1mg/L ANN培养基中,不断地诱导产生不定芽,分化的不定芽转入不含激素的1/2MS培养基中,形成幼苗、然后生根,最后可形成小球茎.幼苗转入1/2MS+0.1mg/L NAA培养基中,幼苗生根,再可形成小球茎.1个唐葛蒲突变株子房,经6—7个月培养,获得了上千株试管苗. 相似文献
8.
9.
Summary Production of microspore-derived embryos from cultured anthers is now a well established technique for the isolation of homozygous lines in many crop plants. We describe here a culture method for embryo induction and plant regeneration from anthers of four sunflower genotypes. For preliminary experiments, anthers of uninucleate microspores were cultured on four types of basal media viz., Murashige and Skoog's MS, Gamborg's B5, Nitsch and Nitsch, and White's W, supplemented with 1.0 mg/l 2,4 dichlorophenoxy acetic acid and 0.5 mg/l 6-benzylaminopurine and 40 g/l sucrose. MS basal medium, being more responsive for embryo induction, was used for further experimentation. To optimise the culture requirement MS basal medium was supplemented with 0.2–2.0 mg/l 2,4 dichlorophenoxy acetic acid and 0.5 and 1.0 mg/l 6-benzylaminopurine. The effect of cold pretreatment, hormone regime and sucrose concentration were tested for embryogenic efficiency. Genotype had a significant effect on the capacity of embryo induction. Addition of silver nitrate (2.5 mg/l), an ethylene inhibitor, stimulated embryo germination. Plantlets were obtained (10–15%) from embryos of only one genotype.Abbreviations 2,4-D
2,4 dichlorophenoxy acetic acid
- NAA
-naphthalene acetic acid
- IAA
indole-3-aceticacid
- BAP
6-benzylaminopurine
- KN
Kinetin
- ABA
abscisic acid
- GA3
gibberellic acid 相似文献
10.
Isolated microspores from six cultivars of Brassica napus and one of B. carinata were cultured in modified Nitsch and Nitsch (NN) medium supplemented with 13% (W/V) sucrose, 0.05 mg/l benzyladenine (BA) and 1.00 mg/l nahpthaleneacetic acid (NAA). Embryogenic responses were observed at cultured temperatures ranging from 22 to 32°C. For most genotypes tested, the highest frequency of embryos occurred at 30°C and 7–54 embryos per anther (approx. 17 000 microspores per anther) developed. Although incubation at 30°C produced the highest frequency of embryos, lower culture temperatures induced better quality embryos. A split temperature culture regime of incubation at 32°C for 3 days followed by incubation at 25°C resulted in both high embryo yields and a high percentage of normal embryos. Plantlet development from microspore-derived embryos appeared to be influenced by both genotype and medium. 相似文献
11.
红掌组织培养与快速繁殖 总被引:12,自引:1,他引:12
红掌叶片在新代培养基上的分化能力与品种和叶片部位有关。组织培养试验表明,最佳诱导培养基为改良Nitsch (NH4NO3 200mg/L)+6-BA 1.0mg/L+2,4-D 0.1mg/L;芽增殖培养基Nitsch (NH4NO3720mg/L)+6-BA 0.5mg/L;生根培养基为Nitsch (NH4NO3720mg/L)。 相似文献
12.
雨堡组织培养的研究 总被引:3,自引:1,他引:2
取雨堡当年生的枝条上饱满的未萌发的侧芽为外植体,培养于MS培养基上,其中附加各种不同种类及浓度的激素。在附加6—BA1.0mg/l的MS培养基上,诱导芽的效果最好;在附加6—BA1.0+NAA0.05+ZT0.1mg/l的MS培养基上,芽的增殖效果最佳;在附加KT0.3+NAA0.05+ZT0.1mg/l或6—BA0.3+NAA0.05+ZT0.1mg/l的MS培养基上,最有利于壮苗;在1/2MS培养基中,附加IBA0.5+NAA0.3ml/l或IAA0.5+NAA0.3mg/l的培养基中,生根效果最佳。试管苗高2—3cm,且具有4—8条短根时,开瓶煅炼3—5天,移入培养土中生长的效果好,种植在保温保湿环境中,试管苗成活率高。 相似文献
13.
Somatic embryos induced from callus cultures of lemongrass [Cymbopogon flexuosus (Nees) Wats.] on Murashige and Skoog medium supplemented with 5 mg/l of 2,4-D, 0.1 mg/l of NAA and 0.5 mg/l of Kn developed into plantlets when plated on a medium supplemented with 3 mg/l of BA, 1 mg/l of GA3 and 0.1 mg/l of NAA. The regeneration potential of callus was retained for more than 2 years on the nutrient medium supplemented with comparatively lower levels of growth regulators (2,4-D at 2 mg/l, NAA at 0.1 mg/l and Kn at 0.25 mg/l). Approximately 30–35 plantlets were produced after two months of culture per 100 mg of callus inoculated. Regenerants were transplanted into soil and transferred to the field for assessment of various morphological and biochemical characteristics. The results of 1 year of field trials showed that plants derived from somatic embryoids were more uniform in all the characteristics examined when compared with the field performance of plants raised through slips by standard propagation procedures. Thus, a procedure has been developed for high frequency long term plant production of lemongrass through in vitro methods.Abbreviations 2,4-D
2,4 -dichlorophenoxyacetic acid
- NAA
-naphthalene acetic acid
- Kn
kinetin
- BA
benzyladenine
- GA3
gibberllic acid
- MS
Murashige and Skoog (1962) basal medium 相似文献
14.
Plant regeneration from callus protoplasts of the forage legume Astragalus adsurgens Pall. 总被引:1,自引:0,他引:1
A reproducible release of viable protoplasts was obtained from friable calli of Astragalus adsurgens. Protoplasts underwent sustained divisions and formed cell colonies when cultured in either liquid or agarose-solidified
Kao and Michayluk (1975) protoplast medium (KM8P) supplemented with 1.5 mg/l 2,4-D, 0.5 mg/l BA and 0.5 M glucose. Compared to liquid culture, agarose bead culture improved division frequency effectively, the two culture systems
showing a plating efficiency of 0.8±0.5% and 6.5±0.7%, respectively. Upon transfer to Murashige and Skoog (1962) medium (MS)
with 1–2 mg/l BA, alone or in combination with NAA or 2,4-D at 0.1 mg/l, the protoplast-derived calli produced complete plantlets
through somatic embryogenesis. The maximum percentage of calli producing somatic embryos (52.5± 2.2%) occurred on MS medium
containing 0.1 mg/l NAA and 1 mg/l BA, whereas the maximum number of calli regenerating plantlets (64.7±6.2) was obtained
on MS medium with 0.1 mg/l NAA and 2 mg/l BA.
Received: 25 April 1997 / Revision received: August 1997 / Accepted: 2 September 1997 相似文献
15.
Timir Baran Jha Satyesh Chandra Roy Gopal Chandra Mitra 《Plant Cell, Tissue and Organ Culture》1982,2(1):11-14
Calli from hypocotyl explant of Cuminum cyminum L. (Cumin) grew rapidly on Gamborg's B5 basal medium with the following supplements, (i) 0.5 mg/l — 2,4-D (ii) 4 mg/l — NAA plus 2 mg/l — Kinetin and (iii) 0.2 mg/l — NAA plus 0.2 mg/l — BAP, whereas calli from leaf explant in these media grew slowly. Hypocotyl and leaf calli produced roots when transferred to basal medium only and shoots in basal medium with 0.5 mg/l NAA and 0.1 mg/l BAP. Ninety percent of the shoots produced roots when they were transferred to half strength MS inorganic salts supplemented with 0.5 mg/l each of IBA and NAA.Fifty to sixty percent of rootless as well as rooted shoots produced terminal umbellate flowers on this medium. 相似文献
16.
Shoot cultures of ipecac, Cephaelis ipecacuanha Rich. were established by inoculating seedling nodal explants onto modified Murashige and Skoog's medium supplemented with 8 mg/l kinetin, 0.05 mg/l NAA and 200 mg/l adenine. Upto 12 new axillary shoots per explant were induced after 12 weeks incubation. Shoot cultures were also established by placing shoot tips on medium containing 0.1–0.25 mg/l NAA with 8 mg/l kinetin for 4 weeks and then to shoot multiplication medium for 8 weeks. The multiplication was maintained over several passages. Shoots were rooted using 2 mg/l IBA and normal plants were re-established. 相似文献
17.
Maize (Zea mays L.) protoplasts obtained from Type I and Type II calli from several genotypes were shown to be capable of synthesizing cell walls and forming small clusters of cells. The medium used also supported cluster formation from protoplasts obtained from root tips. The effects of various additions to the medium (such as casein hydrolysate, coconut water, amino acids, sugars, phytohormones, nitrate, calcium, and dimethylsulfoxide as well as pH variations on cellcluster formation were determined. The method of culture (protoplasts plated in agarose or supported in alginate beads in liquid medium) as well as several components of the medium were found to be critical for microcallus formation. Protoplasts obtained from embryogenic Type I callus and cultured in the medium of C. Nitsch and J.P. Nitsch (1967, Planta 72, 355–370) modified by various additions (NN 67-mod medium) were affected most by various sugars, casein hydrolysate, coconut water, and a combination of the auxins napthalene-1-acetic acid (2 mg/l) and 2,4-dichlorophenoxyacetic acid (0.1 mg/l), and the cytokinin N6-benzylaminopurine (0.5 mg/l). Cluster size in the agarose culture system was from 0.1 to 0.5 mm diameter and in the alginate culture system, up to 2.0 mm diameter. 相似文献
18.
B. Cuenca M. C. San-José M. T. Martínez A. Ballester A. M. Vieitez 《Plant cell reports》1999,18(7-8):538-543
Internodal and leaf segments from pedunculate oak (Quercus robur L.) seedlings were used as explant source to induce somatic embryogenesis. Auxin treatment influenced embryogenic response, which
only occurred in explants initially cultured on media containing 4 mg/l naphthaleneacetic acid (NAA) and different benzyladenine
(BA) concentrations. After 6 weeks of culture on induction medium, the explants were transferred to medium supplemented with
0.1 mg/l BA and 0.1 mg/l NAA, and 4 weeks later, they were subcultured in a growth-regulator-free medium, in which somatic
embryos arose through indirect regeneration on the surface of a nodular callus. Somatic embryos were induced in explants of
two out of four seedling provenances. The induction frequency ranged from 16% in leaf explants to 4% in internodal explants.
Somatic embryos developed two cotyledons, which were translucent or opaque-white in appearance, but anomalous morphologies
were also observed. Different embryogenic lines were established and maintained by repetitive embryogenesis in multiplication
medium containing 0.1 mg/l BA plus 0.05 mg/l NAA. These results indicate that tissues from explants other than Q. robur zygotic embryos are able to produce embryogenic cultures.
Received: 14 July 1998 / Revision received: 2 November 1998 / Accepted: 6 November 1998 相似文献
19.
金红花的组织培养快速繁殖研究 总被引:3,自引:0,他引:3
金红花顶芽或腋芽培养在MS基本培养基中。研究植物激素及培养基的物理性质对器官形成的影响。试验结果表明:芽增殖培养基以附加BA1.0mg/l和NAA0.2mg/l为好。生根培养基为1/2MS+NAA0.1mg/1。糊状培养基有利于苗的生长,试管有根苗和无根苗移栽均获得高的成活率。 相似文献
20.
Explants from 13-d old pepper (Capsicum annuum, L. cv. Early California Wonder) seedlings were cultured in Murashige and Skoog (MS) medium supplemented with different levels
of 1-naphthalene acetic acid (NAA) and 6-benzylamino purine (BAP). Multiple shoot-buds proliferated from the cut surfaces
of cotyledon, shoot-tip and hypocotyl explants in one month. The best NAA to BAP combinations (mg/l: mg/l) for multiple shoot-bud
regeneration of the above three explant types were 0.1 ∶ 5.0, 0.0 ∶ 5.0, and 0.1 ∶ 10.0, respectively. Root explants did not
express any new morphogenetic response in all hormonal combinations tested. Regenerated shoot-buds were excised from the explants
and cultured in 1/2X or 1X MS medium supplemented with different levels of Indole-3-acetic acid (IAA) or NAA. When cultured
in full strength MS medium with 0.5 mg/l IAA or 0.4 mg/l NAA, 70% of the buds rooted in one month. Plantlets were established
successfully ex vitro under greenhouse mist and grown to maturity. 相似文献