A new saponin from seeds of Amoora rohituka

Phytochemical examination of the seeds of Amoora rohituka resulted in the isolation and identification of a new saponin, stigmasta-5,24(28)-dien-3β-O-β-d-glucopyranosyl-O-α-l-rhamnopyranoside.     

A triterpenoidal saponin from roots of Acanthus illicifolius

A new triterpenoidal saponin has been isolated from the ethanolic extract of Acanthus illicifolius. It was shown to be [α-l-arabinofuranosyl-(1 → 4)-β-d-glucuronopyranosyl(1 → 3)]-3β-hydroxy-lup-20 (29)-ene.     

一种百合甾体皂苷纯度及糖链结构核磁共振法分析

采用多种NMR分析技术,首次对百合甾体皂苷(25R,26R)-26-甲氧基螺甾烷-5-烯-3β-α-L-鼠李糖-(1→2)-[β-D-葡萄糖-(1→6)]-β-D-葡萄糖苷的1H和13C NMR信号进行了全归属,特别是应用选择性的1D TOCSY和1D NOESY核磁共振分析技术,对该化合物1中的氢谱信号严重重叠的糖链进行了详细的分析,提出了一套对甾体皂苷糖链信号进行全归属的核磁共振法.在确认其结构的基础上,建立了核磁共振法(1H NMR)测定该化合物1的纯度,给出了完整的实验条件,线性回归系数为0.9998,重复性实验RSD为0.58％,稳定性实验RSD为0.24％,操作简单、快速准确,且不需要其它对照品,是中药化学对照品纯度研究的一个有益补充.     

A triterpenoid saponin from Ficaria ranunculoides tubers

One of the minor saponins extracted from the tubers of Ficaria ranunculoides and purified by fermentation may be 3-O-(α-arabinopyranosyl-1′)28-O-[β-glucopyranosyl → 6″(α-rhamnopyranosyl-1? → 4″) β-glucopyranosyl-1″]-hederagenin. On the basis of chemical degradation and spectral analysis, the structure of this new saponin is proposed.     

A new naturally acetylated triterpene saponin from Nigella sativa

A new glycosylated triterpene 1 was identified as 3-O-[β-d-xylopyranosyl-(1→3)-α-l-rhamnopyranosyl-(1→4)-β-d-glucopyranosyl]-11-methoxy-16-hydroxy-17-acetoxy hederagenin from an ethanolic extract of seeds of Nigella sativa Linn. Identification of the naturally acetylated saponin was based on chemical and spectroscopic analyses including FABMS, ¹H, ¹³C, and 2D NMR and DEPT. The saponin was a penta hydroxy pentacyclic triterpene, in which one hydroxyl group was acetylated and other one was methylated naturally.     

Steroidal saponins and homoisoflavanone from the aerial parts of Sansevieria cylindrica Bojer ex Hook.

Phytochemical study on the methanolic extract of Sansevieria cylindrica aerial parts lead to the isolation, characterization and structure elucidation of a new steroidal saponin, 1β-hydroxy-kryptogenin-1-O-α-l-rhamnopyranosyl-(1 → 2)-α-l-arabinopyranoside (1), a new homoisoflavanone, (3S)-3,7-dihydroxy-8-methoxy-3-(3′,4′-methylenedioxybenzyl) chroman-4-one (2) and the known saponin alliospiroside A (3). To the best of our knowledge, the genin 1β-hydroxy-kryptogenin is reported here for the first time. The structures of the new compounds were determined by UV, IR, EIMS, HRESIMS together with 1D (¹H and ¹³C) and 2D (HSQC and HMBC) NMR spectral analysis. The isolated compounds 1–3 were tested for their radical scavenging activity (DPPH). Compound 2 exhibited activity compared to that of ascorbic acid as a standard. The cytotoxicity of the isolated compounds and the standard doxorubicin was tested against the three human tumor cell lines HT116, MCF-7 and PC-3. The results showed that the isolated compounds were inactive.     

滇黄精中一个三萜皂苷的NMR研究

从滇黄精新鲜根茎中首次分离得到一个三萜皂苷,拟人参皂苷-F11。本文利用1D NMR和2D NMR对其碳、氢信号进行了全归属,并对文献中报道的碳谱数据进行了纠正。     

大叶吊兰中一个新的甾体皂甙

从百合科植物大叶吊兰（Ｃｈｌｏｍｐｈｙｔｕｍ ｍａｌａｙｅｎｓｅ）根茎中分离得到１个新的呋甾烷型甾体皂甙,命名为大叶吊兰甙Ｅ（ｃｈｌｏｒｏｍａｌｏｓｉｄｅ Ｅ）。经波谱和化学方法证明明其结构为：２６－０－β－Ｄ－ｇｌｕｃｏｐｙｒａｎｏｓｙｌ－２２－ｈｙｄｒｏｘｙ－２５（Ｓ）－５ａ－ｆｕｒｏｓｔａｎ－１２－ｏｘｏ－３β,２６－ｄｉｏｌ－３－β－Ｄ－ｘｙｌｏｐｙｒａｎｏｓｙｌ（１→３）〖α－Ｌ－ａｒａ     

Steroidal saponins from the fresh tubers of Ophiopogon japonicus

Eleven novel furostanol saponins, named ophiofurospisides C–E, G–N (1–3, 5–12), one new spirostanol saponin, named ophiopogonin R (13), were isolated from the fresh tubers of Ophiopogon japonicus. Their structures were determined on the basis of spectroscopic techniques (1D and 2D NMR) and HRESIMS. The isolated furostanol saponins possessed two sugar chains located at C-3 and C-26, respectively. Six furostanol saponins (1, 5–9) with disaccharide moiety linked at position C-26 of the aglycone were rare in the plant kingdom.     

A new steroidal saponin from Allium ampeloprasum var. porrum with antiinflammatory and gastroprotective effects

A new steroidal saponin was isolated from the bulbs of Allium ampeloprasum var. porrum. On the basis of chemical conversions and detailed analyses of ¹H and ¹³C NMR spectra including 2D NMR spectroscopic techniques, its structure was established as 3-[(O-β-d-glucopyranosyl-(1 → 3)-β-d-glucopyranosyl-(1 → 2)-O-[O-β-d-glucopyranosyl-(1 → 3)]-O-β-d-glucopyranosyl-(1 → 4)-β-d-galactopyranosyl)oxy]-2,6-dihydroxy-(2α,3β,5α,6β,25R)-spirostane. Results of the present study indicated that the steroidal saponin showed haemolytic effects in the in vitro assays and demonstrated antiinflammatory activity and gastroprotective property using in vivo models.     

Study on the Structure of a New Triterpene Saponin B from Polygala japoniea Houtt

A new triterpene saponin B has been isolated from the earial parts of Polygala japonica Houtt in folk-lore medicine. Its molecular: C48H78O20, m.p. 199–202℃, [α]D23+30.0 (C, 0.5, CH3OH). Acidic hydrolysis of this saponin gave a sapogenin (2α, 3α, 24-tri-hydroxyolean-12-ene-28-oic acid) and D-glucose. The structure of saponin B was elucidated as 28-O- [β-D-glucopyranosyl (1→2) -β-D-glucopyranosyl (1→2) -β-D-glucopy- ranosyl] 2α, 3α, 24-trihydroxyolean-12-ene-28-oic acid mainly by 13C-NMR, MS and some chemical transfomations.     

Steroidal alkaloid saponins and steroidal saponins from Solanum surattense

A rare 16β-H steroidal alkaloid saponin (1), an avenacoside-type saponin (2), two steroidal saponins (4, 5), one revised-structure steroidal saponin (3) and six known compounds (6-11) were isolated from aerial parts of Solanum surattense Burm. f. Their structures were established on the basis of physical data, as well as by using spectroscopic (HRESIMS, 1D and 2D NMR), and chemical analysis methods. Compounds 1 and 11 showed cytotoxicity against A549 cell line with IC₅₀ values of 20.3 and 15.7 μM, respectively.     

空心莲子草中具杀灭钉螺活性皂甙成分的研究

从空心莲子草全草(Alternanthera philoxeroides)的水溶性部位分离鉴定出五个五环三萜及去甲三萜皂甙类成分(1～5).其中两个皂甙(2和5)显示出强的杀灭钉螺活性.化合物2～5为首次从该属植物中发现.     

异柱五加叶中齐墩果烷型皂苷的研究(英文)

利用柱色谱从异柱五加 (Acanthopanax sieboldianus forma albeofolium Yook) 叶的甲醇提取液中分离出四个齐墩果烷型皂苷类化合物.通过波谱方法(1H NMR、13C NMR、2D NMR和FAB-MS)鉴定它们分别为kalopanax-saponin B (1)、acanthopanax saponin CP3(2)、kalopanax-saponin A (3) 和sieboldianoside A (4).     

Isolation and Indentification of Protosaponins from Fresh Rhizomes of Dioscorea zingiberensis Wright

Five steroidal chemical compounds were isolated from the fresh rhizomes of Dioseorea zingiberensis Wright gathered from Sichuan Province. Their chem ieal structures have been elucidated as diosgenin palmitate [Ⅰ]; β-sitosterol [Ⅱ]; gra- cillin [Ⅲ]; protogracillin [Ⅳ] and 3-O-{α-L-rhamnopyranosyl (1→3)-[β-D-glucopy- ranosyl (1→2)]-β-D-glucopyranosyl}-26-O-{β-D-glucopyranosyl}-diosgenin [Ⅴ]. [Ⅴ]is a new steroidal saponin, named protozingiberensissaponin.     

Anti-inflammatory activity of a new triterpenoid saponin from carthamus tinctorius linn

A new bioactive triterpenoid saponin 3β-O-[β-D-xylopyranosyl(1 → 3)-O-β-D-galactopyranosyl]-lup-12-ene-28 oic acid-28-O-α-L-rhamnopyranosyl ester compound (A), was isolated from the methanolic fraction of the roots of this plant by various colour reactions, chemical degradations and spectral analysis. Compound (A) showed anti-inflammatory activity.     

A triterpenoid saponin possessing antileishmanial activity from the leaves of Careya arborea

Bioguided-fractionation of the methanol extract of the leaves of Careya arborea led to isolation of a triterpenoid saponin, designated arborenin, and characterized as 3-O-beta-D-glucopyranosyl(1-->2)-beta-D-glucopyranosyl-2 alpha,3beta-dihydroxy-taraxast-20-en-28-oic acid (1), together with desacylescin III (2). The structures were determined on the basis of extensive 2D NMR spectroscopic analysis. The saponin showed in vitro antileishmanial activity against Leishmania donovani (strain AG 83).     

一种提纯粗茶皂素的简易方法

对粗茶皂素的提取纯化工艺进行了研究。以质量分数为70％的粗茶皂素为原料,经2％NaOH溶解、盐酸酸析、95％乙醇溶解、丙酮沉析工艺得到精制茶皂素。检测结果表明,茶皂素质量分数大于95％,提取率大于80％,这种简便易行的工艺得到的茶皂素可作为开发新型植物灭螺剂的原料。     

Effects of oligosaccharins on callus growth and saponin content of Panax notoginseng

This work provides some evidences for the saponinproduction of Panax notoginseng callus by using biologi-cally active,wall-related oligosaccharins.In anappropriate concentration,three kinds of oligosaccharinsstimulated saponin formation or callus growth.Theconcentration of DO,GO and CO for saponin productionof Panax notoginseng callus culture were 15ppm,15ppmaud 20ppm respectively by comparing saponin yield.Itwas very obvious for DO to increase saponin contentwhen the concentration was 10ppm,and for GO tostimulate callus growth when the concentration was20ppm.It would be a good way to produce saponin byusing oligosaccharins in large scale culture in thefuture.     

Chemical Constituents of the Roots of Vernonia cumingiana Benth.

To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G （1）, was isolated from the roots of Vernonia cumingiana Benth. （Compositae）. The structure of vernonioside G was elucidated using spectral methods, particularly two-dimensional nuclear magnetic resonance analysis. Together with the new compound, eight known compounds were also isolated and identified from the roots of V. cumingiana, among which, VE-1 （2） and 24-methylenelanost-9（11）- en-3β-ol acetate （3） were assigned NMR data for the first time and compound 3 was obtained as a natural product from a plant for the first time.