Chemical Constituents from Roots of Jatropha curcas

Thirteen compounds were isolated from the roots of datropha curcas L. Combining the determination of physico-chemical constants and spectral analyses (IR, 1H-NMR, 13C-NMR, EIMS, FABMS), the structures of the compounds were identified as 5α-stigmastane-3, 6-dione (1), nobiletin (2), β-sitosterol (3), taraxerol (4), 2S-tetracosanoic acid glyceride-1(5),5-hydroxy-6,7-dimethoxycoumarin (6), jatropholone A (7), jatropholone B (8), 6-methoxy-7-hydroxycoumarin (9), caniojane (10), 3-hydroxy-4-methoxybenzaldehyde (11), 3-methoxy-4-hydroxybenzoic acid (12) and daucosterol (13). Among them, compound 5 is a new compound which has never been reported in China and abroad, compound 1, 2, 9, 10, 11, 12 were first time isolated from the plant, 7 and 8 are a pair of stereoisomers which can be inverted in dilute basic solution. 10 is a diterpenoid containing peroxide bridge.     

Chemical Structures of Macrocalyxoformin B,C and E

From the leaves of Rabdosia macrocalyx(Dunn) Hara form (Labiatae), three new diterpenoids, macrocalyxoformin B, C and E, were isolated together with oleanolic acid, β-sitosterol and daucosterol. Their structures were established as (1), (3) and (7) by spectroscopic and chemical evidence respectively. Macrocalyxoformin B and C were shown to have inhibitory action on Hela cells, Staphylococcus aureus in vitro.     

麻疯树皮的化学成分研究

从麻疯树(Jatrophac curcus L.)的树皮中分离得到12个化合物,经理化常数和波谱鉴定(IR,1H NMR,13C NMR,EI-MS)分别为二十六酸甲酯(1),β-谷甾醇(2),蒲公英萜醇(3),蒲公英甾醇(4),伪蒲公英甾醇(5),curcusones A(6),curcusones B(7),jatropholone A(8),jatropholone B(9),3,3′,4-三甲氧基鞣花酸(10),胡萝卜甙(11),蔗糖(12)。其中化合物1,4,5,10,12为首次从该植物中分离得到。     

Chemical compounds and pharmacological effects of Rabdosia excisa

Many kinds of diterpenoids have been isolated from Rabdosia spp.Some of them have anti-microbial effects,counteract inflammation,and inhibit tumor progression activities.We conducted the present study in order to look for bioactive compounds in the medicinal plant Rabdosia excisa.In this study,five compounds were isolated from R.excisa;they were oridonin,isokamebakaurin,oleanolie acid,ursolic acid,and β-sitosterol.In order to identify the function of the extracts,the activity of antibiotics,antioxidation,and immunity test were carried out against these functions.Prospective results were observed in all of the tested items.     

Study on the Chemical Constituents of Fluggea virosa(Roxb. ex Willd.) Baill

Seven compounds have been isolated from Fluggea virosa (Roxb. ex Willd) Baill. They are identified as virosecurinine, norsecurinine, two new norsecurinine type alkaloids: fluggeaine ether, fluggeainol and hentriacontane, β-sitosterol, glochidenol.     

Mono- and triglycosylsterols from the leafy stem of rice

Two sterylglycosides have been isolated from rice plants and identified as β-d-glucopyranosyl (1 → 3)-sitosterol and β-d-glucopyranosyl(1     

The Chemical Constituents of Myripnois dioica Bunge

Five compounds have been isolated from Myripnois dioica Bunge, a plant native to China. They are identified as nonacosane, taraxerol acetate, β-amyrin acetate, β-amyrin and β-sitosterol.     

Studies on the Constituents of Aristolochia mollissima Hance

Ten constituents have been isolated from the ethanolie extract of the stems and leaves of Aristolochia mollissima Hanee. Based on speetrai analysis and the determination of physieo-ehemieal constants they have been identified as: (A1) aristoloehie acid A, (A2) vanillie acid, (A3) aristoloehie acid D, (B1) pahnitone, (B2) n-triacontanol, (B3) β-sitosterol, (B1) aristololaetam, (B11)6-methoxy aristololaetam, (B7) β- sitosterol D-glueoside, (B8) stearic acid. A1 and B1 have been found to have an inhibition action of implantation in mice.     

8-hydroxydihydrochelerythrine and arnottianamide from roots of Toddalia asiatica

In addition to hexacosanoic acid, β-sitosterol and arnottianamide, 8-hydroxydihydrochelerythrine, a new benzophenanthridine alkaloid has been isolate     

Naphthazarins from Arnebia hispidissima

Besides arnebin-7, alkannin acetate, alkannin isovalerate, alkannin and β-sitosterol, a new isohexenylnaphthazarin, alkannin β-hydroxyisovalerate, has been isolated from the roots of Arnebia hispidissima.     

Isolation and structural elucidation of two new labdane diterpenoids from the aerial part of Roylea cinerea

Two new labdane diterpenoids, cinereanoid A (1) and cinereanoid B (2), along with five known compounds, calyone (3), pilloin (4), 1-methylindole-3-carboxaldehyde (5), β-sitosterol (6) and stigmasterol (7) were isolated from the aerial parts of Roylea cinerea (Lamiaceae). The new structures were determined by using IR, MS, 1D, 2D NMR spectroscopy. The structure of both new compounds was further confirmed by single crystal X-ray crystallographic analysis. In this study we have also reported single crystal X-ray structure of compound 3 which unambiguously confirmed the relative stereochemistry of tertiary hydroxyl and methyl groups, as it was not established by earlier report. Compounds 4 and 5 were isolated for the first time from this plant. In view of very few reports about this species, this report has increased the phytochemical knowledge about R. cinerea.     

A lignan from Lonicera hypoleuca

A new lignan characterised as (-)-4-hydroxy-2,6-di-(4′-hydroxy-3′-methoxy)phenyl-3,7-dioxabicyclo-(3.3.0)octane along with n-10-nonacosanol, scopoletin, syringic acid, β-sitosterol and its glucoside, has been isolated from the aerial parts of Lonicera hypoleuca. The stereochemistry of the lignan has been established by its spectroscopic analysis and those of its derivatives, and by its conversion to (+)-pinoresinol. β-Sitosterol-β-D-glucoside displayed good spasmolytic activity.     

Hela细胞的microRNA芯片分析及β-谷甾醇的抑癌路径预测

天然产物β-谷甾醇对子宫癌的治疗具有较显著的功效,目前已被应用到癌症的临床治疗中,它对人体的正常细胞的毒副作用要远小于顺铂这类传统药物。目前人们对β-谷甾醇的抗癌机理知之甚少。本研究主要利用TaqMan MicroRNA As-say芯片技术对β-谷甾醇和顺铂处理过的Hela细胞进行分子水平检测,分析两种药物处理前后Hela细胞中microRNA表达水平的差异,得到在β-谷甾醇和顺铂两种药物作用下同时差异表达的microRNA,并结合多种数据库的使用进行靶标预测,频数分析及功能富集分析得到与癌症相关的靶标STK4/MST1,从生物信息学角度上推测β-谷甾醇可能抑癌路径。     

竹叶马兜铃化学成分的研究

<正> 竹叶马兜铃Aristolochia bambusifolia.C.F Liang sp.nov.是最近在广西隆林首次发现的马兜铃科马兜铃属植物新种。该属植物大部分为药用或民间有名草药。文献曾报导广西马兜铃属植物有12种,但无此种,民间亦未使用。其化学成分尚未研究过,因此我们对其块根化学成分进行系统研究是很有意义的。     

(−)-Maglifloenone,a novel spirocyclohexadienone neolignan and other constituents from Magnolia liliflora

A new neolignan designated (?)-maglifloenone and the known one futoenone, both of which contain the rarely occurring spirocyclohexadienone skeleton, have been isolated together with the tetrahydrofuranolignan (+)-veraguensin, an optically inactive tertiary base taspine and β-sitosterol from the leaves and twigs of Magnolia liliflora. The structure and stereochemistry of (?)-maglifloenone have been deduced from the spectral data and the mass fragmentation of (?)-maglifloenone and futoenone have been rationalized. This is the first report of two neolignans of spirocyclohexadienone skeleton and of taspine from the Magnoliaceae family and the second report of the natural occurrence of futoenone.     

A cinnamoyl pyrrolidine amide from Piper peepuloides

Analysis of a petrol extract of the leaves of Piper peepuloides resulted in the isolation of a new cinnamoyl pyrrolidine amide characterized as Z-pyrrolidine-1-3-(6-methoxy-1,3-benzodioxol-5-yl)-1-oxo-2-propenyl along with peepuloidin, 2-methoxy-4,5-methylenedioxy-Z-cinnamoyl piperidide, β-sitosterol and β-sitosterol glucoside.     

Studies on the Aliphatic Constituents trom the Pollen of Typha angustifolia L.

Five aliphatic compounds were isolated from the pollen of Typha angustifolia L. (Typhaceae). Two of them are new compounds. On the basis of spectroscopic analysis the structure of compound Ⅰ and Ⅱ was elucidated as 7-methyl-4-triacontanone and 6-tritriacontanol, respectively. Pentacosane (Ⅲ)and β-sitosterol palmitate (Ⅳ)also been isolated and identified. The other one (Ⅴ) have not been identified yet. The compound Ⅳ showed significant effect in lowering the serum cholesterol.     

A flavonoid with cytotoxic activity and other constituents from Centaurea africana

A new acylated flavonoid glucoside named algerianin 1 and a new as natural product, 4′-methyl gossypetin 2, together with 10 known compounds, isovanillic acid ethyl ester, β-sitosterol, β-sitosterol 3-O-glucoside, a mixture of α and β-amyrin, 3′-hydroxyflindulatin, chrysoeriol, jaceidin, corniculatusin and centaurein were isolated from the ethanolic extract of the flowering and aerial parts of Centaurea africana Lamk var. africana (Bonnet) M., an endemic species to Algeria and Tunisia collected from El-Kala in the eastern Algeria. The structures were established by chemical and spectral analysis, mainly HREIMS, ESIMS, UV and NMR experiments (GOESY, COSY, ROESY, HSQC and HMBC). Algerianin showed cytotoxicity against the human myeloid leukaemia cell line HL-60.     

The Chemical Constituents of the Rhizome of Acanthopanax Obovatus

From the stem of Acanthopanax obovatus Hoo., an endemic species collected from Huang-long county, Shaanxi Province, eight crystal constituents(Ⅰ-Ⅷ)have been isolated and the structure of the new natural compound Ⅷ were determined as n-butyl-α-D-mannopyranoside by spectroscopic data and chemical method. The known compounds Ⅰ-Ⅶ were identified as β-sitosterol, daucosterol, sesamine, dl-syrigaresinol, acanthoside D, stearic acid and laccerol on the basis of spectral evidence and comparison of authentic samples.     

Beneficial effects of beta-sitosterol on glucose and lipid metabolism in L6 myotube cells are mediated by AMP-activated protein kinase

AMP-activated protein kinase (AMPK) is an energy-sensing enzyme that has been implicated as a key factor for controlling intracellular lipids and glucose metabolism. β-Sitosterol, a plant sterol known to prevent cardiovascular disease was identified from Schizonepeta tenuifolia to an AMPK activator. In L6 myotube cells, β-sitosterol significantly increased phosphorylation of the AMPKα subunit and acetyl-CoA carboxylase (ACC) with stimulating glucose uptake. In contrast, β-sitosterol treatment reduced intracellular levels of triglycerides and cholesterol in L6 cells. These effects were all reversed by pretreatment with AMPK inhibitor Compound C or LKB1 destabilizer radicicol. Similarly, β-sitosterol-induced phosphorylation of AMPK and ACC was not increased in HeLa cells lacking LKB1. These results together suggest that β-sitosterol-mediated enhancement of glucose uptake and reduction of triglycerides and cholesterol in L6 cells is predominantly accomplished by LKB1-mediated AMPK activation. Our findings further reveal a molecular mechanism underlying the beneficial effects of β-sitosterol on glucose and lipid metabolism.