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1.
Meares K Dawson DA Horsburgh GJ Glenn TC Jones KL Braun MJ Perrin MR Taylor TD 《Molecular ecology resources》2009,9(1):308-311
Forty-three microsatellite loci originally isolated in Grus americana and G. japonensis were tested for polymorphism in the blue crane (G. paradisea). Amplified products were sequenced in the blue crane to aid in the design of blue crane-specific primers. When characterized in 20 unrelated blue crane individuals from South Africa, 14 loci were polymorphic, with each locus displaying between 2 and 7 alleles. Eight polymorphic loci were characterized in the grey-crowned crane (Balearica regulorum) and ten in the wattled crane (G. carunculatus). 相似文献
2.
We determined how the presence of heterospecific individuals in the vicinity of a focal individual affects the behavior of
two critically endangered species of cranes on their wintering grounds at Cheolwon in the Korean Demilitarized Zone. The red-crowned
crane, Grus japonensis, is larger than the white-naped crane, Grus vipio, and it dominates the white-naped crane in aggressive interactions. We showed that the dominant species increases foraging
activity in the presence of the subordinate species presumably because of scrounging of food from the subordinate. Because
interspecific interactions may affect avian endangerments these behavioral findings should be taken into account when managing
winter refuges for the two endangered crane species. 相似文献
3.
The blue mussel Mytilus trossulus occurs in the Pacific and in the North Atlantic. We developed and characterized six microsatellite loci for Baltic M. trossulus. Seventeen microsatellite loci were screened, of which six were polymorphic. The number of alleles among 50 individuals ranged
from 3 to 13 and the observed and expected heterozygosity were 0.09–0.46 and 0.34–0.86, respectively. The loci were also tested
for cross amplification in M. edulis, in which four of the six microsatellite loci were successfully amplified. 相似文献
4.
Nina Yasuda Satoshi Nagai Chunlian Lian Masami Hamaguchi Takeshi Hayashibara Kazuo Nadaoka 《Conservation Genetics》2008,9(4):1011-1013
We isolated 11 polymorphic microsatellites from blue coral (Heliopora coerulea), whose conservation and management are of great concern. The number of alleles ranged from 3 to 20 with an average of 5.5,
and the observed and expected heterozygosities ranged from 0.115 to 0.833 and from 0.371 to 0.915, respectively. These loci
are useful for conservation genetics in H. coerulea populations. 相似文献
5.
We describe eight new polymorphic microsatellite loci (5 dinucleotide and three trinucleotide) for the eastern phoebe (Sayornis phoebe) to complement five previously published loci. The number of alleles per locus ranged from 3 to 15 and observed heterozygosities
ranged from 0.33 to 0.91. Preliminary screening revealed that loci were polymorphic in other Tyrannidae: Empidonax virescens (n = 10), Tyrannus tyrannus (n = 10), Tyrannus vociferans (n = 5), and Tyrannus melancholicus (n = 28). 相似文献
6.
In order to study hybridisation, taxonomic boundaries and phylogeography in the genus Pseudopanax (Araliaceae), we developed seven novel microsatellite loci from enriched genomic libraries of P. lessonii and P. crassifolius. These loci were characterised in 16 individuals from a single population of P. crassifolius, and displayed 2–11 alleles per locus. Observed heterozygosities ranged from 0.063 to 0.688. Most loci were polymorphic in
the closely related Pseudopanax species, and several loci amplified widely across the Araliaceae. 相似文献
7.
Achnatherum sibiricum is a threaten and toxic perennial bunchgrass mainly in the north of China. We developed 11 polymorphic microsatellite loci
from A. sibiricum by combining biotin capture method. After validating and scoring, these loci were polymorphic in a test population with the
range of alleles from four to 13 per locus. The observed and expected heterozygosities ranged from 0.1649 to 0.5624 and from
0.3071 to 0.8826, respectively. All 11 microsatellite markers were in Hardy–Weinberg equilibrium. These polymorphic microsatellites
will be useful for genetic diversity analysis and population structure construction for A. sibiricum. 相似文献
8.
Abdul Muneer PM Gopalakrishnan A Musammilu KK Mohindra V Lal KK Basheer VS Lakra WS 《Molecular biology reports》2009,36(7):1779-1791
Random amplified polymorphic DNA (RAPD) and microsatellite markers were applied to evaluate the genetic variation in endemic
and endangered yellow catfish, Horabagrus
brachysoma sampled from three geographic locations of Western Ghat, South India river systems. In RAPD, of 32 10-mer RAPD primers screened
initially, 10 were chosen and used in a comparative analysis of H. brachysoma collected from Meenachil, Chalakkudy and Nethravathi River systems. Of the 124 total RAPD fragments amplified, 49 (39.51%)
were found to be shared by individuals of all 3 populations. The remaining 75 fragments were found to be polymorphic (60.48%).
In microsatellites, six polymorphic microsatellite loci were identified by using primers developed for Pangasius
hypophthalmus, Clarias
macrocephalus and Clarias
gariepinus. The identified loci were confirmed as microsatellite by sequencing after making a clone. The nucleotide sequences of 6 loci
were published in NCBI genbank. The number of alleles across the six loci ranged from 4 to 7 and heterozygosities ranged from
0.07 to 0.93. The mean number of alleles and effective number of alleles per locus were 5.00 and 3.314, respectively. The
average heterozygosity across all investigated samples was 0.72, indicating a significant deficiency of heterozygotes in this
species. RAPD and microsatellite methods reported a high degree of gene diversity and genetic distances depicted by UPGMA
dendrograms among the populations of H. brachysoma. 相似文献
9.
The redlegged earth mite (Halotydeus destructor) and the blue oat mite (Penthaleus major) are major pests of pastures and crops in southern Australia. Reproductive modes, migration rates and levels of differentiation between populations were investigated using allozyme electrophoresis. Collections were made throughout Victoria and a sample was also obtained from Western Australia. Three enzyme loci were polymorphic in H. destructor (Mdh-1, Mdh-2 and Idh). Genotype frequencies of these loci did not differ between phenotypic males and females, providing no evidence for haplodiploidy. Allele frequencies were in Hardy-Weinberg equilibrium, indicating that H. destructor is diploid and sexual. This was confirmed via crosses between males and females. Allele frequencies differed between Victorian sites, although F statistics indicated little differentiation over all loci. A sample from Western Australia did not differ in allele frequencies from the Victorian sites. Four polymorphic loci were found in P. major (Mdh-1, Mdh-2, Idh and Gpi). Only a few multilocus genotypes occurred in a sample, indicating that P. major is parthenogenic. No male P. major were found in this study. A number of colour morphs were also identified and a genetic association between genital plate colour and clonal type was found in one population of P. major. Two different body colour morphs were associated with different clonal types. 相似文献
10.
Xiaolin Liao Dan Wang Xiaomu Yu Weitao Li Lei Cheng Jianwei Wang Jingou Tong 《Conservation Genetics》2007,8(4):1003-1007
Rare minnow (Gobiocypris rarus) is an endangered small fish endemic to upper reach of the Yangtze River. From a (GT)n enriched genomic library, 32 microsatellites were isolated and characterized. Nineteen of these loci were polymorphic in
a test population with alleles ranging from 2–7, and observed and expected heterozygosities from zero to 0.8438, and 0.2679
to 0.8264, respectively. In the cross-species amplifications, 13 out of 19 polymorphic loci were found to be also polymorphic
in at least one of the 7 closely related species of the subfamily Gobioninae. These polymorphic microsatellite loci should
provide sufficient level of genetic diversity to evaluate the fine-scale population structure in rare minnow and its closely
related species for the conservation purpose. 相似文献
11.
Three subspecies of sandhill crane (Grus canadensis) are recognized in the Midcontinental population, the lesser (Grus c. canadensis), Canadian (G. c. rowani), and greater (G. c. tabida). Blood samples collected on the population's primary spring staging area in Nebraska, U.S.A., were used to resolve the genetic relationship among these subspecies. Phylogenetic analysis of 27 G. canadensis, by DNA sequencing of a 675 bp region of the mtDNA, supports the subspecies designations of G. c. canadensis and G. c. tabida. G. c. rowani individuals were intermediate with each of the other two subspecies. Genetic divergence ranged from 6.5 to 14.5% between G. c. canadensis and G. c. tabida, 0.5 to 6.6% within G. c. canadensis, and 0.1 to 6.0% within G. c. tabida. Sufficient DNA for analysis was obtained from shed feathers indicating a source of genetic material that does not require the capture or sacrifice of the birds. Other genetic markers and methods, including satellite telemetry, are required for obtaining detailed information on crane distributions as needed to establish effective management units for the MCP. 相似文献
12.
The flagfish (Jordanella floridae) is commonly used in studies of wetlands ecology. Here we describe the isolation of ten microsatellite loci, six of which
were polymorphic. The observed number of alleles ranged from 4 to 24 and observed heterozygosities ranged from 0.59 to 0.81
for the polymorphic loci. The isolation of these markers will enable estimations of genetic diversity in natural populations. 相似文献
13.
The Australian freshwater cod genus, Maccullochella is represented by three species: Murray cod, M. peelii
peelii, eastern freshwater cod, M. ikei, and trout cod, M.macquariensis. Seven novel microsatellite loci from M. ikei and six previously published loci from M. peelii peelii were tested on wild populations of Murray, eastern and trout cod. Levels of polymorphism varied between species with 13 loci
polymorphic in Murray cod, 9 in trout cod and 7 in eastern cod. Observed heterozygosities ranged from 0.053 to 0.842. This
suite of microsatellite loci will facilitate future studies of the genetic status of wild and hatchery bred populations of
Maccullochella. 相似文献
14.
Michael G. Gardner Juan J. Sanchez Rachael Y. Dudaniec Leah Rheinberger Annabel L. Smith Kathleen M. Saint 《Conservation Genetics》2008,9(1):233-237
We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity
ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation
for conservation strategies for the endangered T. adelaidensis. 相似文献
15.
Fragmentation of natural habitats of the common vole (Microtus arvalis) provides an excellent model system to study the consequences of restricted gene flow and small population sizes for isolated
populations. Here we describe the isolation and characteristics of 10 autosomal and one X-linked microsatellite marker. These
new markers were tested in 24 voles from a natural population in eastern Germany. Loci were highly polymorphic with numbers
of alleles per locus ranging from three to 26 and expected heterozygosities from 0.51 to 0.97. All loci except for the X-linked
locus Mar105 followed Hardy–Weinberg expectations. Cross-species amplifications revealed that most loci were polymorphic as
well in M. agrestis, M. thomasi, and M. pennsylvanicus. 相似文献
16.
With the aim of elaborating a breeding plan on a captive stock of the highly endangered Adriatic sturgeon (Acipenser naccarii), a total of 10 polymorphic microsatellite loci were isolated from an enriched library. The results of cross amplification
of additional 8 loci previously isolated from A. oxyrinchus, A. fulvescens and Scaphyrinchus platorhynchus are also reported. Given the tetraploid condition of the species the genetic variability was estimated basing on the number
of alleles per individuals and the average band sharing. 相似文献
17.
We isolate six highly polymorphic microsatellite loci for the Puerto Rican crested anole (Anolis cristatellus) from a genomic library enriched for CA repeats. The number of alleles per locus ranged from 14 to 19, with levels of observed
heterozygosity ranging from 0.60 to 0.73. Most of these loci were successfully cross-amplified in other members of the cristatellus species group (A. evermanni, A. gundlachi, A. krugi, A. stratulus), but levels of polymorphism were lower. 相似文献
18.
Isolation and characterization of microsatellite markers for the endangered <Emphasis Type="Italic">Taxus yunnanensis</Emphasis> 总被引:1,自引:0,他引:1
Ying-Chun Miao Jian-Rong Su Zhi-Jun Zhang Hui Li Jing Luo Ya-Ping Zhang 《Conservation Genetics》2008,9(6):1683-1685
Eleven polymorphic microsatellite loci were characterized for the endangered conifer Taxus yunnanensis. Eight loci were isolated through SSR-anchored PCR, one locus was developed by cross-species amplification tests, while the
last two loci were obtained from cross-species microsatellite sequences available in GenBank. Variability of these markers
was tested in 48 individuals collected from its main distribution range in Southwest China. The number of alleles per locus
ranged from 2 to 9, and the observed and expected heterozygosity varied from 0.000 to 0.625 and from 0.062 to 0.853, respectively.
Ten of these eleven loci were significantly deviated from Hardy–Weinberg expectation, except TS07 which showed a distinct
heterozygote excess. The availability of these new polymorphic microsatellite markers will provide an ideal marker system
for detailed population genetics studies in T. yunnanensis and potentially also for closely related species. 相似文献
19.
Nine simple sequence repeat (SSR) loci were isolated from an ectomycorrhizal fungus Suillus bovinus by dual-suppression PCR. Three of the SSR loci isolated were polymorphic. The number of alleles per locus was between two
and seven, and expected heterozygosities ranged from 0.087 to 0.740. One of these was confirmed to be species specific and
codominant, suggesting applicability for the analysis of belowground population structure and gene flow of S. bovinus. 相似文献
20.
Marta Dubreuil Federico Sebastiani Maria Mayol Santiago C. González-Martínez Miquel Riba Giovanni G. Vendramin 《Conservation Genetics》2008,9(6):1665-1668
Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight
primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals
from 5 different populations (10–26 samples per population) were used to estimate genetic diversity parameters. High levels
of genetic diversity, with values ranging from 0.533 to 0.929 (6–28 alleles per SSR) were found. No linkage disequilibrium
between pairs of loci was detected. All loci but one showed significant departures from Hardy–Weinberg equilibrium. Excess
of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size
and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical
scales and to monitor gene flow. 相似文献