共查询到20条相似文献,搜索用时 62 毫秒
1.
Background
The presence of gaps in an alignment of nucleotide or protein sequences is often an inconvenience for bioinformatical studies. In phylogenetic and other analyses, for instance, gapped columns are often discarded entirely from the alignment. 相似文献2.
Background
Quality assessment of microarray data is an important and often challenging aspect of gene expression analysis. This task frequently involves the examination of a variety of summary statistics and diagnostic plots. The interpretation of these diagnostics is often subjective, and generally requires careful expert scrutiny. 相似文献3.
Background
C-reactive protein (CRP) is an indicator of inflammation, and is often used in the diagnosis of bacterial infections. It is poorly known whether CRP in bacterial infection is age-dependent. 相似文献4.
Background
Phylogenetic methods are philosophically grounded, and so can be philosophically biased in ways that limit explanatory power. This constitutes an important methodologic dimension not often taken into account. Here we address this dimension in the context of concatenation approaches to phylogeny. 相似文献5.
Lior Shamir Nikita Orlov D Mark Eckley Tomasz Macura Josiah Johnston Ilya G Goldberg 《Source code for biology and medicine》2008,3(1):13
Background
Biological imaging is an emerging field, covering a wide range of applications in biological and clinical research. However, while machinery for automated experimenting and data acquisition has been developing rapidly in the past years, automated image analysis often introduces a bottleneck in high content screening. 相似文献6.
Ian Walsh Alberto JM Martin Catherine Mooney Enrico Rubagotti Alessandro Vullo Gianluca Pollastri 《BMC bioinformatics》2009,10(1):195-19
Background
Proteins, especially larger ones, are often composed of individual evolutionary units, domains, which have their own function and structural fold. Predicting domains is an important intermediate step in protein analyses, including the prediction of protein structures. 相似文献7.
Background
Vertebrate genes often appear to cluster within the background of nontranscribed genomic DNA. Here an analysis of the physical distribution of gene structures on human chromosome 7 was performed to confirm the presence of clustering, and to elucidate possible underlying statistical and biological mechanisms. 相似文献8.
Tomas Radivoyevitch 《Biology direct》2009,4(1):50
Background
The Hill coefficient characterizes the extent to which an enzyme exhibits positive or negative cooperativity, but it provides no information regarding the mechanism of cooperativity. In contrast, models based on the equilibrium concept of mass action can suggest mechanisms of cooperativity, but there are often many such models and often many with too many parameters. 相似文献9.
Shoji Fukamachi Masato Kinoshita Kouichi Aizawa Shoji Oda Axel Meyer Hiroshi Mitani 《BMC biology》2009,7(1):64-10
Background
Animals utilize a wide variety of tactics to attract reproductive partners. Behavioral experiments often indicate an important role for visual cues in fish, but their molecular basis remains almost entirely unknown. Studies on model species (such as zebrafish and medaka) allow investigations into this fundamental question in behavioral and evolutionary biology. 相似文献10.
Tom Lenaerts Jesper Ferkinghoff-Borg Francois Stricher Luis Serrano Joost WH Schymkowitz Frederic Rousseau 《BMC structural biology》2008,8(1):43
Background
Efficient communication between distant sites within a protein is essential for cooperative biological response. Although often associated with large allosteric movements, more subtle changes in protein dynamics can also induce long-range correlations. However, an appropriate formalism that directly relates protein structural dynamics to information exchange between functional sites is still lacking. 相似文献11.
A. Cadiere B. Couturaud J. Boismard P. Le Cann A. Gérard A. Mas C. Faye L. Garrelly B. Roig 《Journal of applied microbiology》2013,115(1):290-297
Aims
Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly‐l ‐lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real‐time PCR quantification.Methods and Results
This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0·99 ± 0·04 and Y intercept when x = ?0·02 ± 0·28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16 600‐fold 1 l of sample and to detect and quantify down to 750 GC l?1 and 7500 GC l?1, respectively).Conclusions
To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water.Significance and Impact of the Study
This study gives valuable advance in the methods of concentration and diagnosis of virus in water. 相似文献12.
Radim Cegan Gabriel AB Marais Hana Kubekova Nicolas Blavet Alex Widmer Boris Vyskot Jaroslav Doležel Jan Šafář Roman Hobza 《BMC plant biology》2010,10(1):180
Background
The evolution of sex chromosomes is often accompanied by gene or chromosome rearrangements. Recently, the gene AP3 was characterized in the dioecious plant species Silene latifolia. It was suggested that this gene had been transferred from an autosome to the Y chromosome. 相似文献13.
Background
Interpreting the results of high-throughput experiments, such as those obtained from DNA-microarrays, is an often time-consuming task due to the high number of data-points that need to be analyzed in parallel. It is usually a matter of extensive testing and unknown beforehand, which of the possible approaches for the functional analysis will be the most informative 相似文献14.
Anina Ritterband-Rosenbaum Mark S Christensen Mette Kliim-Due Line Z Petersen Betina Rasmussen Jens B Nielsen 《BMC neurology》2011,11(1):150
Background
Children diagnosed with spastic Cerebral Palsy (CP) often show perceptual and cognitive problems, which may contribute to their functional deficit. Here we investigated if altered ability to determine whether an observed movement is performed by themselves (sense of agency) contributes to the motor deficit in children with CP. 相似文献15.
Background
Large molecular sequence databases are fundamental resources for modern bioscientists. Whether for project-specific purposes or sharing data with colleagues, it is often advantageous to maintain smaller sequence databases. However, this is usually not an easy task for the average bench scientist. 相似文献16.
Debra T Burhans Lakshmi Ramachandran Jianxin Wang Ping Liang Hugh G Patterton Michael Breitenbach William C Burhans 《BMC evolutionary biology》2006,6(1):58
Background
Coordinately regulated genes often physically cluster in eukaryotic genomes, for reasons that remain unclear. 相似文献17.
Signal processing of heart signals for the quantification of non-deterministic events 总被引:1,自引:0,他引:1
Background
Heart signals represent an important way to evaluate cardiovascular function and often what is desired is to quantify the level of some signal of interest against the louder backdrop of the beating of the heart itself. An example of this type of application is the quantification of cavitation in mechanical heart valve patients. 相似文献18.
Background
In expressed sequence tag (EST) sequencing, we are often interested in how many genes we can capture in an EST sample of a targeted size. This information provides insights to sequencing efficiency in experimental design, as well as clues to the diversity of expressed genes in the tissue from which the library was constructed. 相似文献19.