共查询到20条相似文献,搜索用时 10 毫秒
1.
Alcohol fermentation has traditionally been carried out in aqueous environments because of the ready solubility of reactant (sugar) and product (ethanol). However, extraction of the product ethanol into a nonmiscible phase can result in kinetic benefits due to reduced inhibition of the fermentation reactions. In this study, we report the development of a novel simultaneous saccharification and extractive fermentation (SSEF) process. Ethanol productivity was increased by up to 65% over conventional (nonextractive) fed-batch simultaneous saccharification systems when calculated on the basis of aqueous phase volume. The amount of water required for SSEF reactions was dramatically reduced from that required for conventional SSF. In batch SSEF reactors with 2.5% aqueous phase, 50% conversion of 25% (aqueous phase concentration) Solka Floc could be achieved in 48 h using 2 FPU/g cellulase. (c) 1996 John Wiley & Sons, Inc. 相似文献
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Mohammed A. Malek Najmul A. Chowdhury Shinpei Matsuhashi Shoji Hashimoto Tamikazu Kume 《Mycoscience》1994,35(1):95-98
The effect of radiation pasteurization of sugar cane bagasse and rice straw and fermentation using various strains of fungi were studied for upgrading of cellulosic wastes. The initial contamination by fungi and aerobic bacteria both in bagasse and straw was high. The doses of 30 kGy for sterilization and 8 kGy for elimination of fungi were required. Irradiation effect showed that rice straw contained comparatively radioresistant microorganisms. It was observed that all the fungi (Hericium erinacium, Pleurotus djamor, Ganoderma lucidum, Auricularia auricula, Lentinus sajor-caju, Coriolus versicolor, Polyporus arcularius, Coprinus cinereus) grow extending over the entire substrates during one month after inoculation in irradiated bagasse and rice straw with 3% rice bran and 65% moisture content incubated at 30°C. Initially, sugar cane bagasse and rice straw substrates contained 39.4% and 25.9% of cellulose, 22.9% and 26.9% of hemicellulose, and 19.6% and 13.9% of lignin + cutin, respectively. Neutral detergent fibre (NDF) values decreased significantly in sugar cane bagasse fermented byG. lucidum, A. auricula andP. arcularius, and in rice straw fermented by all the 8 strains of fungi. Acid detergent fibre (ADF) values also decreased in bagasse and rice straw fermented by all the fungi.P. arcularius, H. erinacium, G. lucidum andC. cinereus were found to be the most effective strains for delignification of sugar cane bagasse. 相似文献
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Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast 总被引:4,自引:0,他引:4
Simultaneous saccharification and fermentation (SSF) studies were carried out to produce ethanol from lignocellulosic wastes (sugar cane leaves and Antigonum leptopus leaves) using Trichoderma reesei cellulase and yeast cells. The ability of a thermotolerant yeast, Kluyveromyces fragilis NCIM 3358, was compared with Saccharomyces cerevisiae NRRL-Y-132. K. fragilis was found to perform better in the SSF process and result in high yields of ethanol (2.5-3.5% w/v) compared to S. cerevisiae (2.0-2.5% w/v). Increased ethanol yields were obtained when the cellulase was supplemented with beta-glucosidase. The conversions with K. fragilis were completed in a short time. The substrates were in the following order in terms of fast conversions: Solka floc > A. leptopus > sugar cane. 相似文献
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A. Bhadra J. M. Scharer M. Moo-Young 《World journal of microbiology & biotechnology》1986,2(3):349-358
Summary Anaerobic digestion is an extensively used bioconversion process to produce gaseous fuel from native lignocellulosic materials. It consists essentially of two steps; acidogenesis and methanogenesis. Most conventional anaerobic digesters are single-stage systems. Animal waste, agricultural residues, sewage sludge and industrial effluents are suitable as feedstock. Biogas productivity in single stage digestion ranges from 0.5–1.5 m3/m3/day at mesophilic (20–40°C) and 1.0–2.5 m3/m3/day at thermophilic (40–60°C) temperatures, and about 30–50% of the volatile solids are converted to biogas. In two-stage systems, acidogenesis is separated from methanogenesis, which improves cellulose hydrolysis and process efficiency. Recent advances in digester design include the introduction of the upflow anaerobic sludge blanket, and fluidized-bed and fixed-film bioreactors, which are operated at much higher loading. Process efficiency can be as high as 97%. An overview of these technologies is presented.
Resumen La digestión anaeróbica es un proceso de bioconversión muy utilizado para producir gas combustible a partir de materiales de tipo lignocelulósico. El proceso consta esencialmente de dos etapas acidogénesis y metaogénesis. La mayoría de los digestores de tipo convencional son de una sola etapa. Como material de partida pueden utilizarse residuos dé ganado, agrícolas, lodos de depuradoras y efluentes industriales. La producción de biogás en un digestor de una sola etapa va desde 0.5–1.5 m3/m3 día cuando el proceso transcurre a temperaturas mesófilas hasta 1.0–2.5 m3/m3 día cuando lo hace a temperaturas termófilas, transformándose entre un 30 y un 50% de los sólidos volátiles en biogás. En los sistemas con dos etapas la acidogénesis se separa de la metanogénesis con lo cual se mejora la hidrólisis de la celulosa y por lo tanto la eficiencia del proceso. Entre los recientes adelantos en el diseño de digestores cabe incluir la introducción de una manta de flujo ascendente para fangos anaeróbicos y bioreactores con camas fluidas y de lámina fija que pueden usarse con cargas mayores. La eficiencia del proceso puede llegar entonces hasta 97%. En este trabajo se pasa revista a todas estas nuevas tecnologías.
Résumé La fermentation anaérobie est un procédé de bio-conversion très utilisé pour produire des carburants gazeux à partir de ligno-cellulose. Elle comporte essentiellement deux étapes: acidogenèse et méthanogenèse. La plupart des digesteurs usuels sont des systèmes à un seul étage. Les déchets animaux, les résidus agricoles, les boues d'épandage et certains effluents industriels sont des substrats appropriés. Dans les fermentations à un seul étage, la productivité en bio-gaz varie entre 0,5 et 1,5 m3 par m3 et par jour pour les températures mésophiles (20–40°C) et de 1,0 à 2,5 pour les températures thermophiles (40–50°C); d'autre part environ 30 à 50% des solides volatils sont convertis en bio-gaz. Dans les systèmes à deux étages, l'acidogenèse est séparée de la méthanogenèse, ce qui améliore l'hydrolyse de la cellulose et l'efficacité du procédé. Les progrès récents concernant la structure des fermenteurs comprennent la circulation du dépôt anaérobie et la réalisation de bioréacteurs à lit fluidisé et à film-fixé, ce qui permet d'opérer avec des charges trés supérieures. L'efficacité peut atteindre 97%. Un aperçu de ces nouvelles technologies est présenté dans l'article.相似文献
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Gang Liu Qiang Zhang Hongxing Li Abdul S. Qureshi Jian Zhang Xiaoming Bao Jie Bao 《Biotechnology and bioengineering》2018,115(1):60-69
Despite the well‐recognized merits of simultaneous saccharification and co‐fermentation (SSCF) on relieving sugar product inhibition on cellulase activity, a practical concomitance difficulty of xylose with inhibitors in the pretreated lignocellulose feedstock prohibits the essential application of SSCF for cellulosic ethanol fermentation. To maximize the SSCF potentials for cellulosic ethanol production, a dry biorefining approach was proposed starting from dry acid pretreatment, disk milling, and biodetoxification of lignocellulose feedstock. The successful SSCF of the inhibitor free and xylose conserved lignocellulose feedstock after dry biorefining reached a record high ethanol titer at moderate cellulase usage and minimum wastewater generation. For wheat straw, 101.4 g/L of ethanol (equivalent to 12.8% in volumetric percentage) was produced with the overall yield of 74.8% from cellulose and xylose, in which the xylose conversion was 73.9%, at the moderate cellulase usage of 15 mg protein per gram cellulose. For corn stover, 85.1 g/L of ethanol (equivalent to 10.8% in volumetric percentage) is produced with the overall conversion of 84.7% from cellulose and xylose, in which the xylose conversion was 87.7%, at the minimum cellulase usage of 10 mg protein per gram cellulose. Most significantly, the SSCF operation achieved the high conversion efficiency by generating the minimum amount of wastewater. Both the fermentation efficiency and the wastewater generation in the current dry biorefining for cellulosic ethanol production are very close to that of corn ethanol production, indicating that the technical gap between cellulosic ethanol and corn ethanol has been gradually filled by the advancing biorefining technology. 相似文献
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Rodríguez Couto S 《Biotechnology journal》2008,3(7):859-870
Biological wastes contain several reusable substances of high value such as soluble sugars and fibre. Direct disposal of such wastes to soil or landfill causes serious environmental problems. Thus, the development of potential value-added processes for these wastes is highly attractive. These biological wastes can be used as support-substrates in solid-state fermentation (SSF) to produce industrially relevant metabolites with great economical advantage. In addition, it is an environmentally friendly method of waste management. This paper reviews the reutilization of biological wastes for the production of value-added products using the SSF technique. 相似文献
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Physicochemical properties of pretreated poplar feedstocks during simultaneous saccharification and fermentation 总被引:2,自引:0,他引:2
Lisbeth Meunier-Goddik Michelle Bothwell Kunruedee Sangseethong Kuakoon Piyachomkwan Yun-Chin Chung Khamphet Thammasouk Djuhartini Tanjo Michael H. Penner 《Enzyme and microbial technology》1999,24(10):77-674
Physicochemical properties of native and dilute acid pretreated (0.6% H2SO4, 10 min, and either 170°C or 180°C) poplar were investigated before and during simultaneous saccharification and fermentation (SSF). SSF duration was 5 days and employed Trichoderma reesei cellulases and Saccharomyces cerevisiae fermentation. Chemical composition (glucan, xylan, lignin), enzyme-accessible surface area (based on solute exclusion), crystallinity index, particle size distribution, particle shape, and enzyme adsorption (cellulase, β-glucosidase) were compared to cellulose conversion. Cellulose conversion varied from 8% for native poplar to 78% for the 180°C-pretreated poplar. The physicochemical properties of native poplar changed little during SSF. In contrast, the physicochemical properties of the 180°C-pretreated feedstock changed markedly. Enzyme-accessible surface area and β-glucosidase adsorption increased by 83% and 65%, respectively, as cellulose was removed from the feedstock. Crystallinity index and particle size (large fraction) decreased by 65% and 93%, respectively. Cellulase adsorption per unit weight increased initially (+45%) followed by a slight decrease (−13%). The same trends were observed, although to a lesser extent, for 170°C-pretreated feedstock. 相似文献
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In this study we describe a model that estimates the extracellular (nonfungal) and overall water contents of wheat grains during solid-state fermentation (SSF) with Aspergillus oryzae, using on-line measurements of oxygen, carbon dioxide, and water vapor in the gas phase. The model uses elemental balances to predict substrate dry matter losses from carbon dioxide measurements, and metabolic water production, water used in starch hydrolysis, and water incorporated in new biomass from oxygen measurements. Water losses caused by evaporation were calculated from water vapor measurements. Model parameters were determined using an experimental membrane-based model system, which mimicked the growth of A. oryzae on the wheat grains and permitted direct measurement of the fungal biomass dry weight and wet weight. The measured water content of the biomass depended heavily on the moisture content of the solid substrate and was significantly lower than the estimated values reported in the literature. The model accurately predicted the measured overall water content of fermenting solid substrate during fermentations performed in a 1.5-L scraped drum reactor and in a 35-L horizontal paddle mixer, and is therefore considered validated. The model can be used to calculate the water addition required to control the extracellular water content in a mixed solid-state bioreactor for cultivation of A. oryzae on wheat. 相似文献
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An experimental apparatus was constructed to measure the structural parameters of organic porous media, i.,e. mechanical strength, air-filled porosity, air permeability, and the Ergun particle size. These parameters are critical to the engineering of aerobic bioconversion systems and were measured for a straw--manure mixture before and after 13 days of in-vessel composting. Porosity was measured using air pycnometry at four (day 0) and five (day 13) moisture levels, with each moisture level tested at a range of different densities. Tested wet bulk densities varied with moisture level, but dry bulk densities generally ranged from 100 to 200 kg m(-3). At each moisture/density combination, pressure drop was measured at airflow rates ranging from 0.001 to 0.05 m sec(-1), representing the range of airflow rates found in both intensive and extensive composting. Measured air-filled porosities were accurately predicted from measurements of bulk density, moisture, and organic matter content. Reductions in air-filled porosity at increasing moisture content were accompanied by an increase in permeability, apparently due to aggregations of fines. This aggregation was quantified by calculating an effective particle size from the Ergun permeability relationship, which increased from 0.0002 m at 50% moisture to 0.0021 m at 79% moisture. The range of airflow velocities reported in composting systems requires consideration of the second-order drag force term, particularly at velocities approaching 0.05 m s(-1) for the higher moisture treatments tested. Calculated permeabilities for the matrix ranged from 10(-10) to 10(-7) m2, varying with both air-filled porosity and moisture. Mechanical strength characterization provided a means to predict the effects of compaction on air-filled porosity and permeability of porous media beds. The results of this investigation extend porous media theory to the organic matrices common in solid-state fermentations and help build a framework for quantitative and mechanistic engineering design. 相似文献
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Ethanol fermentation of acid-hydrolyzed cellulosic pyrolysate with Saccharomyces cerevisiae 总被引:1,自引:0,他引:1
The acid hydrolysis of cellulosic pyrolysate to glucose and its fermentation to ethanol were investigated. The maximum glucose yield (17.4%) was obtained by the hydrolysis with 0.2 mol sulfuric acid per liter pyrolysate using autoclaving at 121 degrees C for 20 min. The fermentation by Saccharomyces cerevisiae of a hydrolysate medium containing 31.6 g/l glucose gave 14.2 g/l ethanol in 24 h, whereas the fermentation of the medium containing 31.6 g/l pure glucose gave 13.7 g/l ethanol in 18 h. The results showed that the acid-hydrolyzed pyrolysate could be used for ethanol production. Different nitrogen sources were evaluated and the best ethanol concentration (15.1 g/l) was achieved by single urea. S. cerevisiae (R) was obtained by adaptation of S. cerevisiae to the hydrolysate medium for 12 times, and 40.2 g/l ethanol was produced by S. cerevisiae (R) in the fermentation with the hydrolysate medium containing 95.8 g/l glucose, which was about 47% increase in ethanol production compared to its parent strain. 相似文献
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The enzymatic hydrolysis of cellulose to glucose is generally a slow reaction. Different pretreatments, such as ball milling to a ?200 mesh or swelling in 1–2% NaOH are reported to increase the reactivity considerably. In this work a fiber fraction from cattle manure was treated in an autoclave for 5–30 min at temperatures ranging from 130–200°C. The reactivity of the cellulose, measured by incubating samples with a commercial cellulase preparation for one hour at 50°C and pH 4.8, was increased by a factor of 4–6 compared to NaOH treatment and 10–12 compared to untreated fiber. The increased reaction rate is probably mostly due to an increase in cellulose availability to enzymatic attack, as structural hemicellulose is hydrolyzed and removed during the treatment. Sugars, produced by hemicellulosis, hydrolysis, will react further to give caramelization products. These side reactions were shown to be suppressed by short treatment times. The treated fiber could support growth of a mixed culture of Trichoderma viride and Candida utilis only after washing, indicating the formation of water soluble inhibitory products during treatment. The treatment with high-temperature steam can probably be used also with other cellulosic materials to increase reactivity. This may be an attractive way to prepare low-valued wastes such as manure fibers, straw, stalks, or corn cobs for fermentation processes to increase the protein content or for use directly as ruminant animal feed. 相似文献
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Ethanol fermentation of acid-hydrolyzed cellulosic pyrolysate with Saccharomyces cerevisiae 总被引:2,自引:0,他引:2
Acid-hydrolysis of cellulosic pyrolysate to glucose and its fermentation to ethanol were investigated. The maximum glucose yield (17.4%) was obtained by the hydrolysis with 0.2 mol/l sulfuric acid using autoclaving at 121 degrees C for 20 min. The fermentation by Saccharomyces cerevisiae of a hydrolysate medium containing 31.6 g/l glucose gave 14.2 g/l ethanol after 24 h, whereas the fermentation of the medium containing 31.6 g/l pure glucose gave 13.7 g/l ethanol after 18 h. The results showed that acid-hydrolyzed pyrolysate could be used for ethanol production. Different nitrogen sources were evaluated and the best ethanol concentration (15.1 g/l) was achieved by single urea. S. cerevisiae (R) was obtained by adaptation of S. cerevisiae to the hydrolysate medium for 12 times, and 40.2 g/l ethanol was produced by it in the fermentation with the hydrolysate medium containing 95.8 g/l glucose, which was about 47% increase in ethanol production compared to its parent strain. 相似文献
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Shabina Yeasmin Shah Md. Asraful Islam Ji-Young Lee 《Preparative biochemistry & biotechnology》2016,46(3):229-237
The efficacy of different concentrations of NaOH (0.25%, 0.50%, 0.75%, and 1.00%) for the pretreatment of rice straw in solid and powder state in enzymatic saccharification and fermentation for the production of bioethanol was evaluated. A greater amount of biomass was recovered through solid-state pretreatment (3.74 g) from 5 g of rice straw. The highest increase in the volume of rice straw powder as a result of swelling was observed with 1.00% NaOH pretreatment (48.07%), which was statistically identical to 0.75% NaOH pretreatment (32.31%). The surface of rice straw was disrupted by the 0.75% NaOH and 1.00% NaOH pretreated samples as observed using field-emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). In Fourier-transform infrared (FT-IR) spectra, absorbance of hydroxyl groups at 1,050 cm?1 due to the OH group of lignin was gradually decreased with the increase of NaOH concentration. The greatest amounts of glucose and ethanol were obtained in 1.00% NaOH solid-state pretreated and powder-state hydrolyzed samples (0.804 g g?1 and 0.379 g g?1, respectively), which was statistically similar to the use of 0.75% NaOH (0.763 g g?1 and 0.358 g g?1, respectively). Thus, solid-state pretreatment with 0.75% NaOH and powder-state hydrolysis appear to be suitable for fermentation and bioethanol production from rice straw. 相似文献
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Vincent S. Chang William E. Kaar Barry Burr Mark T. Holtzapple 《Biotechnology letters》2001,23(16):1327-1333
Simultaneous saccharification and fermentation (SSF) was performed on lime-treated switchgrass and corn stover, and oxidatively lime-treated poplar wood to determine their compatibility with Saccharomyces cerevisiae. Cellulose-derived glucose was extensively utilized by the yeast during SSF. The ethanol yields from pretreated switchgrass, pretreated corn stover, and pretreated-and-washed poplar wood were 72%, 62% and 73% of theoretical, respectively, whereas those from -cellulose were 67 to 91% of theoretical. The lower ethanol yields from treated biomass resulted from lower cellulose digestibilities rather than inhibitors produced by the pretreatment. Oxidative lime pretreatment of poplar wood increased the ethanol yield by a factor of 5.6, from 13% (untreated) to 73% (pretreated-and-washed). 相似文献
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木薯粉同步糖化发酵(SSF)产丁二酸 总被引:1,自引:0,他引:1
【目的】通过优化产琥珀酸放线杆菌GXAS137同步糖化发酵木薯粉产丁二酸的发酵培养基,提高丁二酸产量,降低生产成本。【方法】在单因素试验的基础上,先利用Plackett-Burman试验设计筛选出影响丁二酸发酵的重要参数,再采用正交试验确定重要参数的最佳水平。【结果】价格低廉玉米浆可用作氮源,影响丁二酸产量的重要参数是木薯粉、玉米浆、碱式碳酸镁和糖化酶浓度。最佳条件为(g/L):木薯粉100,玉米浆14,糖化酶2.0 AGU/g底物,碱式碳酸镁75。优化后丁二酸产量达到69.31 g/L,丁二酸得率为90.01%,生产强度为1.44 g/(L·h)。与初始条件(52.34 g/L)相比,丁二酸浓度提高了32.42%。并利用1.3 L发酵罐对SSF与SHF两种发酵工艺进行了比较,SSF丁二酸产量(72.21 g/L)远高于SHF(56.86 g/L)。【结论】产琥珀酸放线杆菌同步糖化发酵木薯粉丁二酸产量高,生产成本低,具有较好的工业化应用前景。 相似文献
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Biotechnological advantages of laboratory-scale solid-state fermentation with fungi 总被引:14,自引:0,他引:14
Despite the increasing number of publications dealing with solid-state (substrate) fermentation (SSF) it is very difficult to draw general conclusion from the data presented. This is due to the lack of proper standardisation that would allow objective comparison with other processes. Research work has so far focused on the general applicability of SSF for the production of enzymes, metabolites and spores, in that many different solid substrates (agricultural waste) have been combined with many different fungi and the productivity of each fermentation reported. On a gram bench-scale SSF appears to be superior to submerged fermentation technology (SmF) in several aspects. However, SSF up-scaling, necessary for use on an industrial scale, raises severe engineering problems due to the build-up of temperature, pH, O2, substrate and moisture gradients. Hence, most published reviews also focus on progress towards industrial engineering. The role of the physiological and genetic properties of the microorganisms used during growth on solid substrates compared with aqueous solutions has so far been all but neglected, despite the fact that it may be the microbiology that makes SSF advantageous against the SmF biotechnology. This review will focus on research work allowing comparison of the specific biological particulars of enzyme, metabolite and/or spore production in SSF and in SmF. In these respects, SSF appears to possess several biotechnological advantages, though at present on a laboratory scale only, such as higher fermentation productivity, higher end-concentration of products, higher product stability, lower catabolic repression, cultivation of microorganisms specialized for water-insoluble substrates or mixed cultivation of various fungi, and last but not least, lower demand on sterility due to the low water activity used in SSF. 相似文献