严重烧伤早期红细胞膜胆固醇含量及Na+-K+-ATP酶活性的变化

目的 :研究严重烧伤患者早期红细胞滤过指数 (EFI)与红细胞膜胆固醇含量、Na K ATPase活性的变化。探讨其在严重烧伤早期中的相互关系及意义。方法 :采用核孔滤膜法测定红细胞滤过指数 (EFI) ,用化学修饰电极法测定红细胞膜胆固醇含量 ,应用定磷法测定红细胞膜Na K ATPase活性。结果 :4 7例严重烧伤早期患者EFI较 6 0例正常对照组下降 (P <0 .0 1) ,红细胞膜胆固醇含量、Na K ATPase活性均高于正常对照组 (P <0 .0 1) ,且红细胞膜胆固醇含量、Na K ATPase活性与EFI呈密切负相关 (rcho =- 0 .871,rATPase =- 0 .80 1,P <0 .0 1)。结论 :严重烧伤早期EFI下降 ,变形性明显减低是导致血液粘度和微循环改变的原因之一 ,红细胞膜胆固醇含量和Na K ATPase活性的变化则是引起EFI下降、变形性减低的重要因素     

Circadian rhythm in the membrane of circulating human blood cells: microviscosity and number of benzodiazepine binding sites. A search for regulation by plasma ions, nucleosides, proteins or hormones

Circadian rhythms in both the number of peripheral type binding sites for benzodiazepines in platelet membranes and the microviscosity of the erythrocyte membrane were demonstrated in 7 healthy men. Neither variable appeared to be linked to each other, or regulated by the plasma concentrations of total or free cortisol, testosterone, potassium, magnesium, calcium, cAMP, cGMP or proteins or by the erythrocytic concentration of magnesium or potassium or by the plasma cAMP:cGMP ratio or by the ratio of intra-erythrocyte:plasma concentrations of potassium or magnesium. A highly significant negative correlation was found between the microviscosity of the erythrocyte membrane and the activity of the membrane-bound enzyme, methyltransferase I. Such a correlation was validated both on raw data and on 24 hr-means (r = 0.84; P less than 0.01). A circadian rhythm in the activity of this enzyme was also demonstrated. Moreover, a highly significant correlation was also found between plasma transcortin concentration (TRC) and microviscosity (r = 0.50, P less than 0.01), and between TRC and methyltransferase I activity (r = 0.61, P less than 0.01). Such findings may constitute clues towards the understanding of the regulation of the circadian rhythm in the fluidity of the red blood cell membrane in man and guide future steps with regard to the role of this rhythm upon the availability of drug binding sites at the cell surface.     

Erythrocyte insulin receptor characteristics and erythrocyte membrane lipid composition in healthy men

The cell membrane plays an important role in the mechanism of insulin action. To test whether erythrocyte insulin receptor characteristics are related to the erythrocyte membrane lipid composition, 11 healthy volunteers were studied. The relationship between insulin binding to erythrocytes, the number of receptors per cell and the affinity of receptors to insulin on the one hand and total phospholipid fatty acid (FA) composition and cholesterol/phospholipid molar ratio in the erythrocyte membrane on the other hand were evaluated. 1. We found a significant negative correlation between specific insulin binding and the proportion of n-6 essential FA in erythrocyte membrane phospholipids, especially linoleic acid (r = -0.82, p less than 0.01) and arachidonic acid (r = -0.73, p less than 0.05). On the other hand, a significant positive correlation between insulin binding and the proportion of nonessential FA (r = +0.65, p less than 0.05) was seen. Number of receptors per cell and the affinity of receptors were not significantly related to phospholipid FA composition. 2. There was no significant correlation between insulin receptor characteristics and the cholesterol/phospholipid molar ratio in the erythrocyte membrane. The data presented support the hypothesis that the FA pattern of membrane total phospholipids may modify the properties of insulin receptors.     

Effects of hypotension on cutaneous and subcutaneous blood flow in anaesthetized humans.

The aim of this study was to determine the effect of controlled hypotension on subcutaneous and cutaneous haemodynamics in humans. Moderate hypotension was achieved with nitroglycerin (NTG) and sodium nitroprusside (SNP) infusion during narconeuroleptanalgesia in seven patients. Subcutaneous and cutaneous blood flow were measured by a superficial and deep heat clearance (HC) technique. The mean arterial pressure (BPa) decreased by 23%-30% and heart rate (fc) increased but only during NTG infusion (+22%; P less than 0.02). Subcutaneous and cutaneous blood flows remained unchanged despite a significant decrease in calculated cutaneous resistance (NTG: -26%, P less than 0.01; SNP: -34%, P less than 0.02] and subcutaneous vascular resistance changed only with SNP (-31%, P less than 0.02). After hypotension was discontinued the subcutaneous blood flow decreased (-13%, P = 0.05), whereas subcutaneous vascular resistance returned to its control values. An inverse relationship was found between fc and BPa (NTG: r = -0.525, P less than 0.01; SNP: r = -0.622, P less than 0.01) as well as with subcutaneous blood flow (NTG: r = -0.653, P less than 0.001; SNP: r = -0.573, P less than 0.01). In addition, we found oscillatory changes in deep HC values which differed in magnitudes (NTG 0.22 (SEM 0.09) W.m-1.degree C-1 vs SNP 0.42 (SEM 0.1) W.m-1.degrees C-1, P less than 0.01) and frequencies (NTG 0.02 (SEM 0.006) Hz vs SNP 0.01 (SEM 0.002) Hz, P less than 0.01). Despite unchanged blood flow, the effects of controlled hypotension on cutaneous and subcutaneous haemodynamics were different depending on the type of drug. These differences may have been related to counterregulatory responses and/or to direct vascular effects.     

Lipid peroxides, spermatozoa quality and activity of glutathione peroxidase in bull semen

In bull semen spontaneous lipid peroxidation measured by the level of endogenous lipid peroxides and the consequences of this process for morphological and biochemical changes was studied. Glutathione peroxidase activity as protective enzyme against peroxidative damage was also determined. Obtained results showed that approximately two thirds of GSH-Px activity in bull semen was non Se-dependent glutathione peroxidase activity. Malonaldehyde (MDA) level was negative correlated with selenium-dependent GSH-Px activity (r = = -0.38, P less than 0.01). Spermatozoa with acrosome entirely lost appeared to increase as the MDA level increased (r = 0.18, P less than 0.05). The negative correlation between Se GSH-Px activity and spermatozoa with acrosome separation from head (r = -0.28, P less than 0.01) and entirely lost (r = -0.21, P less than 0.05) suggest that selenium-dependent GSH-Px plays role in protecting the acrosome against disruption of the acrosomal membrane. The total glutathione peroxidase activity was unrelated to studied variables of bull semen.     

缺血性脑血管病患者血清MMP-8和IL-6水平与颈动脉粥样硬化的关系

目的：分析缺血性脑血管病患者血清MMP-8 和IL-6 的表达水平与颈动脉粥样硬化斑块稳定性的关系。方法：选取2013 年5 月至2014 年10 月我院收治的80 例缺血性脑血管病患者作为研究组,另选取同期在我院门诊接受体检的80 例健康人群作为对 照组。采用双抗体夹心酶联免疫吸附试验(ELISA)检测两组研究对象血清MMP-8 和IL-6 水平。利用Spearman 等级相关性分析方 法分析缺血性脑血管病患者颈动脉斑块的稳定程度与血清MMP-8和IL-6 水平的关系。结果：研究组缺血性脑血管病患者血清中 MMP-8 和IL-6 水平明显高于对照组,两组间数据差异存在统计学意义（P<0.01）。研究组患者中不稳定斑块28 例,稳定斑块32 例,斑块类型介于中间20 例。不稳定斑块患者血清MMP-8 和IL-6 水平高于中间斑块和稳定斑块患者,中间斑块患者血清 MMP-8 和IL-6 水平高于稳定斑块患者,组间比较,差异均具有统计学意义（P<0.05）。Spearman 等级相关性分析结果显示,缺血性 脑血管病患者颈动脉斑块的稳定程度与血清MMP-8 和IL-6 水平存在显著的正相关关系（r=0.71,0.66;P<0.05）。结论：缺血性脑 血管病患者血清MMP-8 和IL-6 水平与颈动脉斑块稳定程度相关。     

Relationship between the intra-erythrocyte sodium composition and the membrane lipoprotein composition among different mammal species

Internal sodium and lipoprotein composition of RBCs of nine mammalian species are measured. A significant correlation can be demonstrated between the erythrocyte mean sodium value of studied species and the membrane protein/lipid ratio (r = 0.80, alpha less than 0.01). Erythrocyte internal sodium can be correlated with membrane-free cholesterol but not with the phospholipid fractions.     

犬心肌缺血早期血小板聚集功能和冠脉侧支循环的改变

本实验在18只麻醉开胸犬观察了急性心肌缺血早期血小板聚集功能和冠脉侧支循环功能的变化。实验结果如下:阻断冠脉后心肌缺血区血液中血小板聚集率(PAgR)增大,血小板计数(PC)减少。缺血50min时,PAgR增大58.7±5.6％,PC减少39.5±23.6％,与对照值有明显差异(均为P<0.01)。与此同时,在控制血压条件下,心肌缺血早期单位压力差下冠脉侧支血流量的变化与对照值无明显差异,而根据Wyatt等公式计算的流经缺血区末梢血管的有效侧支血流量明显降低,缺血50min时较对照值降低23.5±9.7％(P<0.05)。PAgR变化与有效侧支血流量改变呈明显负相关(r=-0.887,P<0.01);冠脉侧支指数与梗塞范围呈明显负相关(r=-0.847,P<0.01)。阻断冠脉前静脉注射血小板聚集功能抑制剂阿斯匹林,可明显减轻上述各项参数的异常变化。这些结果提示,心肌缺血早期血小板聚集功能的异常变化虽然对冠脉侧支血管的血流阻力影响较小,但却使流经缺血区末梢血管的有效侧支血流量明显减小,进而扩大梗塞范围。     

The correlation between the frequency of sister chromatid exchanges and the kinetics of human lymphocyte proliferation: the dependence on the sex of the donor

Sister chromatid exchanges (SCE) and average generation time (AGT) were studied in lymphocytes from 35 donors (23 females and 12 males). A higher SCE frequency was found in lymphocytes from females than from males. Smoking increased SCE frequency in lymphocytes of males, but not of females. No differences in AGTs between males and females were found. Partial correlation coefficients between SCE frequency, AGT values, donor's age and smoking were determined. A statistically significant correlation (r = 0.650, P less than 0.01) between SCE frequency and AGT was found in lymphocytes obtained from females. In lymphocytes from males statistically significant partial correlation coefficients were detected between SCE frequency and AGT (r = -0.696, P less than 0.05), SCE frequency and donor's age (r = 0.770, P less than 0.01), SCE frequency and smoking intensity (r = 0.697, P less than 0.01), AGT value and donor's age (r = 0.882, P less than 0.01), and AGT value and smoking (r = 0.634, P less than 0.05). Thus, considerable differences in number of indices between males and females exist. The present observations together with other studies (D'Souza et al., 1988) suggest that considerations for population monitoring using cytogenetic techniques (ICPEMC Publication No 14) may be supplemented with the recommendation to use (whenever it possible) only males as donors in population studies.     

Variations in use of cardiology services in a health authority: comparison of coronary artery revascularisation rates with prevalence of angina and coronary mortality.

OBJECTIVE: To explore the relation between rates of coronary artery revascularisation and prevalence of angina to assess whether use of health services reflects need. DESIGN: Prevalence of angina symptoms determined by postal questionnaire on 16750 subjects (18 to 94 years). Comparison of data on use of coronary artery revascularisation with prevalence of symptoms and mortality from coronary heart disease. SETTING: Health authority with population of 530000. SUBJECTS: Patients admitted to hospital for coronary heart disease; patients who died; and patients undergoing angiography, angioplasty, or coronary artery bypass graft. Cohort of 491 people with symptoms from survey. MAIN OUTCOME MEASURES: Pearson''s product moment correlation coefficients for relation between variables. RESULTS: Overall, 4.0% (95% confidence interval 3.7% to 4.4%) of subjects had symptoms. Prevalences varied widely between electoral wards and were positively associated with Townsend score (r = 0.79; P < 0.001), as was mortality, but the correlation between admission rates and Townsend score was less clear (r = 0.47; P < 0.01). Revascularisation rate and Townsend score were not associated. The ratio of revascularisation to number experiencing symptoms was inversely related to Townsend score (r = 0.67; P < 0.001). The most deprived wards had only about half the number of revascularisations per head of population with angina than did the more affluent wards. In affluent wards 11% (13/116) of those with symptoms had coronary angiograms compared with only 4% (9/216) in poorer wards (chi 2 = 4.96; P = 0.026). Townsend score also inversely correlated with revascularisations per premature death from coronary heart disease (r = 0.55; P < 0.01) and revascularisations per admission for myocardial infarction (r = 0.47; P < 0.01). CONCLUSION: The use of interventional cardiology services is not commensurate with need, thus exhibiting the inverse care law.     

Electrical admittance for filling of the heart during lower body negative pressure in humans.

To evaluate whether electrical admittance of intracellular water is applicable for monitoring filling of the heart, we determined the difference in intracellular water in the thorax (Thorax(ICW)), measured as the reciprocal value of the electrical impedance for the thorax at 1.5 and 100 kHz during lower body negative pressure (LBNP) in humans. Changes in Thorax(ICW) were compared with positron emission tomography-determined C(15)O-labeled erythrocytes over the heart. During -40 mmHg LBNP, the blood volume of the heart decreased by 21 +/- 3% as the erythrocyte volume was reduced by 20 +/- 2% and the plasma volume declined by 26 +/- 2% (P < 0.01; n = 8). Over the heart region, LBNP was also associated with a decrease in the technetium-labeled erythrocyte activity by 26 +/- 4% and, conversely, an increase over the lower leg by 92 +/- 5% (P < 0.01; n = 6). For 15 subjects, LBNP increased thoracic impedance by 3.3 +/- 0.3 Omega (1.5 kHz) and 3.0 +/- 0.4 Omega (100 kHz), whereas leg impedance decreased by 9.0 +/- 3.3 Omega (1.5 kHz) and 6.1 +/- 3 Omega (100 kHz; P < 0.01). Thorax(ICW) was reduced by 7.1 +/- 1.9 S. 10(-4) (P < 0.01) and intracellular water in the leg tended to increase (from 37.8 +/- 4.6 to 40.9 +/- 5.0 S. 10(-4); P = 0.08). The correlation between Thorax(ICW) and heart erythrocyte volume was 0.84 (P < 0.05). The results suggest that thoracic electrical admittance of intracellular water can be applied to evaluate changes in blood volume of the heart during LBNP in humans.     

Increased erythrocyte catalase activity in patients with hyperthyroidism

Levels of human erythrocyte catalase activity were determined in 38 patients with thyroidal dysfunction. In patients with hyperthyroidism, erythrocyte catalase activities were found to be higher than the levels of normal subjects (P less than 0.001). In hypothyroidism, erythrocyte catalase activities were of the same order as those of normal subjects. Significantly high positive correlation was found between erythrocytes catalase activity and the levels of thyroxine (r = 0.5794, n = 36, P less than 0.001), and slight positive correlation was detected between catalase activity and the levels of triiodothyronine (r = 0.3978, n = 33, P less than 0.05). A decreased erythrocyte catalase activity was observed when erythrocytes lysate was incubated with thyroid hormones. It was suggested that erythrocyte catalase activity had close relationship with thyroid state, however, direct effect of thyroid hormones were not observed on erythrocyte catalase assay system in vitro.     

Effects of alloxan-induced diabetes on hemorheology in rabbits.

Plasma viscosity (PV), apparent whole blood viscosity (WBV), relative blood viscosity (RV) and erythrocyte deformability (filterability) (EDF) were determined in 13 New Zealand White (NZW) rabbits with alloxan induced-diabetes (AID) and 8 normal NZW rabbits, matched for age, sex and weight. AID rabbits were divided into two groups depending on the duration of hyperglycemia (long-term, greater than 6.0 months (n = 7), and short-term, less than or equal to 3.0 months of hyperglycemia, n = 6). Comparing long-term AID rabbits to normal animals, we found significant increases in WBV (P less than 0.001, 0.005 for high and low rates of shear, respectively), and a marked reduction in EDF (P less than 0.001). There was no significant difference in PV between long-term AID and normal rabbits. Conversely, PV was significantly increased in rabbits with short-term diabetes (P less than 0.01) while there was a concurrent significant increase in WBV measured at high and low rates of shear (P less than 0.001, 0.001, respectively). No difference was detected in EDF between normal and short-term AID rabbits. Furthermore, in long-term AID rabbits there was a strongly positive correlation between RV and reduced erythrocyte deformability (r = 0.94, P = 0.006) while WBV strongly correlated with PV (r = 0.92, P = 0.004) in the short-term AID subgroup. We conclude from these data: (1) elevated blood viscosity in long term AID rabbits is associated with reduced erythrocyte filterability; and (2) elevated WBV in short-term AID rabbits is associated with increased PV.     

家燕和金腰燕的卵胚胎心率比较

胚胎心率是衡量胚胎新陈代谢速率的重要指标。鸟类的胚胎心率随新陈代谢的增加而呈上升趋势。对早成性鸟类的种间比较发现,胚胎心率平均值随卵重量的增大而减小,卵体积小的种类具有相对较高的胚胎心率。国内有关野生鸟类胚胎心率的研究较少。2014年5~8月,在黑龙江扎龙国家级自然保护区,利用红外胚胎心率测量仪对两种近缘鸟类家燕(Hirundo rustica,n=14)和金腰燕(Cecropis daurica,n=14)的卵胚胎心率及其变化进行了测量与比较。两种燕均在孵卵的第2天开始出现胚胎心率,并随胚龄增加心率呈上升趋势,但在第8天及第11~14天家燕的胚胎心率显著低于金腰燕(第8天:z=﹣2.602,P=0.009;第11天:z=﹣2.497,P=0.013;第12天:z=﹣2.354,P=0.019;第13天:z=3.424,P=0.001;第14天:z=﹣3.380,P=0.001)。家燕卵胚胎日均增长心率(19.0±3.1)次/min,金腰燕卵胚胎日均增长心率(16.1±3.4)次/min,二者差异不显著(z=﹣1.792,P=0.073)。两种燕的胚胎心率与卵容量和卵重均不存在显著相关性[家燕:卵容量(1.73±0.09)cm3,r=0.192,P=0.511;卵重(1.74±0.09)g,r=0.128,P=0.663。金腰燕:卵容量(1.74±0.08)cm3,r=0.040,P=0.891;卵重(1.51±0.09)g,r=0.054,P=0.855]。这可能表明,卵大小和卵重量对家燕与金腰燕的胚胎心率均影响不明显。     

Insulin receptor changes in type 2 diabetes after short term insulin treatment

We have studied erythrocyte insulin receptor changes before and after 8 days of continuous subcutaneous insulin infusion by a pump in 11 uncontrolled obese non-insulin-dependent diabetics (type 2), diet and drug resistant for at least three months previously. All the patients were hospitalized. On day 1 of the study, their oral hypoglycemic agents were stopped and hypocaloric diet (1000 Kcal/day) was maintained (strictly reinforced). This period of reinforced treatment was not accompanied by correction of hyperglycemia. On day 9 patients were placed for 12 hours on artificial pancreas in order to bring their fasting blood glucose levels down to normal values. Then they were submitted to a continuous subcutaneous insulin infusion (CSII) for the following 8 days. There was a significant decrease in mean fasting plasma glucose (P less than 0.001) and a rise in insulin (P less than 0.05) levels after insulin treatment. Mean specific insulin binding was also significantly increased (P less than 0.01). The increase in binding (with insulin therapy) correlated with the fall in fasting hyperglycemia (r = 0.786, P less than 0.01). In addition, the increase in binding correlated negatively with changes in fasting plasma insulin levels (r = -0.867, P less than 0.01), under treatment, on one hand and with the dose of exogenous insulin administered (r = -0.681, P less than 0.05) on the other hand. There was no correlation between binding and fasting plasma insulin levels (before and after insulin therapy), or between diabetes duration and any of the previous parameters.(ABSTRACT TRUNCATED AT 250 WORDS)     

Increased Na(+)-dependent D-glucose transport and altered lipid composition in renal cortical brush-border membrane vesicles from bile duct-ligated rats.

Erythrocyte membranes of patients with liver disease are characteristically enriched in cholesterol, a change known to impair several carrier-mediated membrane transport functions. In the present study we have assessed whether experimental liver disease can affect the membrane lipid composition and transport function of kidney epithelial cells. Small (about 5%) but significant (P less than 0.01) increases were found in the cholesterol-to-phospholipid molar ratio (C/PL) of rat renal cortical brush-border membrane (BBM) vesicles 3, 8, and 15 days after bile duct ligation which correlated closely with increased fluorescence polarization, i.e., decreased membrane fluidity (r = 0.75, P less than 0.001; n = 27). A lipoprotein-mediated pathogenesis was suggested by the close relationship between BBM C/PL and plasma C/PL (r = 0.69, P less than 0.001). The mean high-affinity Na(+)-coupled D-glucose uptake by BBM vesicles was higher 1, 3, 8, and 15 days after ligation than in non-operated rats, significantly so at 3 and 8 days (611 +/- 37 and 593 +/- 22 vs. 507 +/- 21 pmol/mg protein per 4 sec; P less than 0.05), and was positively correlated with BBM C/PL (r = 0.58, P less than 0.01) and fluorescence polarization (r = 0.41, P less than 0.05). Brief incubation of BBM vesicles from normal rats with cholesterol-rich phospholipid liposomes simultaneously increased BBM C/PL and Na(+)-dependent D-glucose uptake. Stimulation of BBM Na(+)-glucose cotransport in ligated rats was not due to delayed dissipation of the Na+ gradient or to a more rapid development of membrane potential. High-affinity Na(+)-dependent D-glucose uptake kinetics in 3-day bile duct-ligated rats showed a lower Kt, without an alteration in maximum velocity, Vmax, compared to sham-operated animals (0.298 +/- 0.015 vs. 0.382 +/- 0.029 mM; P less than 0.05), whilst the binding dissociation constant, Kd of high-affinity phlorizin binding sites was reduced by ligation (0.453 +/- 0.013 vs. 0.560 +/- 0.015 microM; P less than 0.001). We conclude that an early effect of bile duct ligation is to enrich renal cortical brush-border membranes in cholesterol, thereby decreasing membrane fluidity and stimulating Na(+)-dependent D-glucose uptake by increasing the affinity of the carrier.     

类风湿关节炎患者血脂水平与疾病活动度的相关性研究

目的:观察类风湿关节炎(RA)患者血脂的变化,以及血脂水平与疾病活动度之间的相关性。方法:对71例RA患者和77例正常对照的血脂水平进行回顾性分析,并对RA患者的血脂水平与其疾病活动度进行相关性分析。结果:RA患者的血清总胆固醇(TC)、甘油三酯(TG)水平均高于正常对照组(P0.01),高密度脂蛋白(HDL)水平降低(P0.01)。DAS28评分与TC(r=0.49,P0.01)、TG(r=0.38,P0.01)和LDL(r=0.55,P0.01)呈正相关,与HDL呈负相关(r=-0.57,P0.01),血沉与TC(r=0.26,P=0.03)、TG(r=0.28,P=0.02)呈正相关,C反应蛋白与TC(r=0.65,P0.01)、TG(r=0.30,P=0.01)和LDL(r=0.39,P0.01)均呈正相关。结论:RA患者存在血脂水平异常,且与疾病活动度相关。对血脂进行干预可能改善RA患者的长期预后。     

In vivo neutrophil sequestration within lungs of humans is determined by in vitro "filterability".

Neutrophils are normally delayed in transit through the lung microcirculation, relative to the passage of erythrocytes. This sequestration contributes to a pulmonary pool of neutrophils that may relate to the relative inability of neutrophils to deform compared with erythrocytes when in transit in the pulmonary capillaries. A micropore membrane was used to model the human pulmonary microcirculation, in which cell deformability was measured as the pressure developed during filtration of the cells through the membrane at a constant flow. We demonstrated a significant correlation between in vitro deformability and in vivo lung sequestration of indium-111-labeled neutrophils in 10 normal subjects (r = 0.69, P less than 0.02). In eight patients with stable chronic obstructive pulmonary disease, this relationship was not significant (r = -0.2, P greater than 0.05). Furthermore, in a subject with microscopic pulmonary telangiectasia known to allow significant passage of 30-microns microspheres, neutrophils passed through the lungs without delay. Moreover, neutrophils from patients studied acutely with an exacerbation of chronic obstructive pulmonary disease were temporarily less deformable (P less than 0.01). These studies confirm that cell deformability is an important determinant of the normal neutrophil sequestration within the lungs. Changes in cell deformability may alter the extent of this sequestration.     

Serum E-selectin and erythrocyte membrane Na+K+ ATPase levels in patients with rheumatoid arthritis

We conducted this study to assess serum soluble E-selectin (sE-selectin) levels and erythrocyte membrane Na(+)K(+) ATPase activity in patients with rheumatoid arthritis (RA) and correlate the levels with disease activity. Levels of sE-selectin were measured in the serum of 20 patients with RA and 20 control subjects by an enzyme-linked immunosorbant assay. Na(+)K(+) ATPase activity was determined by a colorimetric method in RA patients and healthy controls. There were no statistically significant differences between the two groups with respect to demographic data such as age and sex (p > 0.05). The serum levels of sE-selectin, ESR and C-reactive protein (CRP) in RA patients were significantly higher than in healthy controls (p < 0.001). Erythrocyte membrane Na(+)K(+) ATPase activity was significantly lower in the RA group than in the control group (p < 0.001). Correlation analysis revealed significant positive correlations between soluble E-selectin and ESR (r = 0.457; p < 0.05) and CRP (r = 0.682; p < 0.01) levels. There were statistically significant negative correlations between erythrocyte membrane Na(+)K(+) ATPase activity and ESR (r = -0.450; p < 0.05) and CRP (r = -0.446; p < 0.05) levels. Additionally, a significant negative correlations between sE-selectin and Na(+)K(+) ATPase activity was observed (r = -0.80; p < 0.001). These results show that decreases in erythrocyte membrane Na(+)K(+) ATPase activity and increases in sE-selectin are observed in RA, and that increased levels of sE-selectin may also reflect disease status or activity.     

DTI和功能磁共振成像对精神分裂症患者脑功能连接强度的临床研究

摘要 目的：探讨DTI和功能磁共振成像对精神分裂症患者脑功能连接强度的临床研究。方法：选取2017年9月-2021年3月在我院精神科就诊的精神分裂患者96例。将纳入的96例患者作为精神分裂组,选择同时期招募的96例健康志愿者作为对照组。使用FMRIB软件库进行结构数据预处理和脑解剖网络构建,并进行相关的分析。结果：精神分裂组较对照组枕叶模块功能连接强度增强,较对照组皮质模块和额顶叶模块功能连接强度减弱（P<0.05）,精神分裂组与对照组的默认模块和中央模块功能连接强度比较无差异（P>0.05）。精神分裂组与对照组各模块的结构连接强度比较无差异（P>0.05）。精神分裂组较对照组默认模式模块和中央模块的结构-功能连接程度增加,精神分裂组较对照组枕叶模块和皮质模块的结构-功能连接程度降低（P<0.05）,额顶叶模块结构-功能连接程度两组比较无差异（P>0.05）。在精神分裂症组中,枕叶模块的结构-功能连接评分与疾病持续时间（r=-0.528,P=0.006）和PANSS总体症状相关（r=-0.174,P=0.003）,皮质模块的结构-功能连接评分也与PANSS整体症状（r=-0.405,P=0.034）显著负相关。皮质模块的结构-功能连接评分与疾病持续时间之间显著负相关（r=-0.336,P=0.029）。结论：精神分裂症患者的脑功能连接强度（功能模块水平上结构-功能耦合）发生改变,其结构-功能耦合的畸变与精神分裂症的临床特征相关。