Chromatography and Biological Stains: II. Column Chromatographic Separation of the Colored Materials in Commercial Sudan III

Commercial Sudan III was separated into various fractions by the usual column chromatographic technics using Celite-silicic acid as the adsorbent and commercial petroleum ether (B) as the solvent. By analysis, using both spectrophotometric and paper strip chromatographic methods, it was possible to identify fractions from the commercial sample, including several fractions which were indubitably very highly purified Sudan III. In addition several other fractions containing mixtures of colored components have been isolated.     

Chromatography and Biological Stains: III. Comparison of the Fat Staining Efficiency of Fractions of Commercial Sudan III Separated by Column Chromatography

Fractions of commercial Sudan III which were separated by column chromatography were compared for fat staining efficiency. Paraffin sections of chromated mouse liver tissue and sections of both fresh and formalin-fixed rat liver tissue or Musca domestica larvae, cut with the freezing microtome, were used. Evidence is presented that a sample of very highly purified Sudan III has no ability to render a fat stain in fresh, formalin-fixed or chromated tissue. However, certain other fractions from the commercial sample, some completely devoid of Sudan III, had good staining characteristics. It is concluded that some substance or substances, other than Sudan III, is responsible for the staining action of the commercial dye.     

东北白眉蝮蛇毒细胞毒组分的分离制备及活性鉴定

用离子交换层析和凝胶过滤法从东北白眉蝮蛇毒中分离出一种具有较强的细胞毒活性的蛋白质。这种蛋白质对肿瘤细胞的细胞毒作用元选择性,但各种肿瘤细胞的敏感性有一定差别。对正常人体细胞也有一定的细胞毒性作用,与肿瘤细胞相比,敏感性较低。这种蛋白质加热到56℃,30分钟,细胞毒活性稳定。4℃及-20℃保存4个月,细胞毒活性稳定。PH值低于4及高于9时,可使其完全失活。     

Commentary: The Life And Times Of V. M. Bekhterev

The provocative article that follows has been translated as part of our endeavors to uncover the contributions of V. M. Bekhterev to social psychology. For these introductory comments we rely most heavily on two sources available in English, mainly the work of Alex Kozulin (1984) and V. Gerver (1932), a junior colleague of Bekhterev's. Other valuable material was provided by the work of A. L. Schniermann (1930), another of Bekhterev's junior associates. These and other writings yield a picture of an outstanding Russian scientist who published about six hundred works on anatomy and physiology, nervous and mental diseases, biology and pedology, philosophy and reflexology; who established an astonishing number of research units and laboratories, ranging from the enormous Psychoneurological Institute in St. Petersburg to small laboratories for research on vocational counseling, children's neuropathology, etc.; and who founded several important journals. In time, he should probably become generally acknowledged as one of the founders of social psychology as well.     

Spectrophotometric Characteristics and Assay of Biological Stains III. The Xanthenes

In this continuation paper of the work on the chemical and spectrophotometic characteristics of commercial stains, data on the xanthene dyes are presented. In the xanthene group of dyes, it has been found possible to assay pyronin B, eosins B and Y and ethyl eosin by spectrophotometric means. Phloxine B, rose Bengal, and erythrosin B are assayed by die color acid precipitation method. Typical absorption curves are given for these dyes as well as representative spectral and assay data.     

Psychological Problems Of Dialogue In Light Of The Ideas Of M. M. Bakhtin And A. A. Ukhtomskii

Comparative examination of the scientific and philosophical ideas of Mikhail Mikhailovich Bakhtin and Aleksei Alekseevich Ukhtomskii is of interest from the standpoint of both the psychological theory of communication and the practice of psychological counseling, psychotherapy, and upbringing. Development of the applied areas of psychology poses the tasks of understanding the particular person and his individual problems and offering psychological assistance in the process of communication. To deal with these questions it is not sufficient to have scientific psychological training, i.e., knowledge of the general laws of personality development and communication; a capacity for personal communication as an aid to resolving human life problems is also necessary.     

紫菀种质资源的评价与分析

紫菀具有重要的药用价值,为我国传统常用中药材,开展紫菀种质资源的鉴定和评价,是提高紫菀产量和品质的前提和基础。通过田间性状调查和室内分析,对取自河北安国和安徽亳州的44份紫菀种质进行性状和品质评价,并对种质遗传多样性进行聚类分析。结果显示:影响种苗质量三因素的变异程度为茎毛数>茎粗>芽距,室内发芽试验结果同样显示种苗的茎毛数对发芽率的影响最大,芽距最小;不同种源紫菀间的叶宽差异不明显,不同种源紫菀间的其他性状如叶长、株高和生长势等性状都表现不同程度的变化,不同种源间的干重和紫菀酮含量差异明显,都表现出极显著水平;不同种源间叶长、叶宽和株高与生长势呈显著的正相关,干重和生长势呈显著的负相关性,紫菀的干重和紫菀酮的含量呈负相关性;根据相似系数进行的聚类结果表现出明显的地域性,该研究明确了不同地区的紫菀为适应当地的生态环境,在遗传上发生了适应性的分化、变异,这为优良品种的选择和选育提供了有利的理论依据。     

Selection in Reference to Biological Groups. V. Analysis of Full-Sib Groups

Previous studies in this series have dealt with the consequences of truncation selection operating with respect to random groups within which geno-full-sib group structure. The objective is to compare the results of individual and group selection as these methods operate on populations of random versus typic interaction may exist. The present study deal with a non-random, full-sib groups. In almost all comparisons individual selection is found to be qualitatively and quantitatively superior when used in conjunction with full-sib groups. This is due to the fact that the change in gene frequency for full-sib groups is a function of both direct and associate effects, whereas with random groups it is a function of direct effects only. With regard to group selection the efficiency is invariably superior when selection operates on non-random, full-sib rather than random groups.