表油菜素内酯对拟南芥细胞分化的影响

研究了表油菜素内酯（ｅｐｉ－ＢＲ）对拟南芥细胞体外分化的影响．表明ｅｐｉ－ＢＲ不仅能促进愈伤组织的增殖,而且还能有效地诱导愈伤组织转绿,继而分化绿芽和长成小植株,其诱导频率高达７０％以上。电镜观察表明,ｅｐｉ－ＢＲ诱导的转绿细胞中的叶绿体发育正常。     

表油菜素内酯对拟南芥细胞分化的影响

研究了表油菜素内酯对拟南芥细胞体外分泌的影响，表明ｅｐｉ－ＢＲ不仅能促进愈伤组织的增殖，而且还能有效地诱导愈伤组织转绿，继而分化绿芽长成小植株，其诱导频率高达７０％以上，电镜观察表明，ｅｐｉ－ＢＲ诱导的转绿细胞中的叶绿体发育正常。     

表油菜素内酯对月季切花衰老的影响

0.5mg·L~(-1)表油菜素内酯明显缓解水分对月季切花的胁迫,促进切花体内水分平衡的改善和还原糖含量的增加,抑制花瓣溶质外渗,从而延缓切花衰老。     

表油菜素内酯对油菜幼苗生长及其可溶性糖和蛋白质含量的影响…

表油菜素内酯处理油菜幼苗,可明显促进下胚轴伸长生长,增加子叶面积,同时降低蛋白质含量及子叶中可溶性糖含量,ＳＤＳ－ＰＡＧＥ检测蛋白结果表明,ｅｐｉＢＲ处理后,下胚轴和子叶中的蛋白组分均发生明显的改变。     

表油菜素内酯对西瓜生长和产量性状的影响（简报）

表油菜素内酯明显促进三叶期西瓜幼苗生长,提高其叶绿素含量和光合作用速率,开花期喷雾处理能增强座果和提高产量20％左右,并可延缓叶片衰老。     

表油菜素内酯对玉米果穗发育的影响（简报）

在玉米雌穗小穗和小花分化期,全株喷施0.01 ppm表油莱素内酯(BR)能促进幼穗发育,使幼穗的生长速度加快、吐丝提前,籽粒的败育率减少,从而导致玉米产量的提高。     

苦参组培快繁技术体系的初步研究

采用正交实验设计(L934)用MS、White和改良MS培养基,分别加入不同质量浓度的玉米素(ZT)、6-苄基氨基腺嘌呤(6-BA)、异戊烯基腺嘌呤(2-ip)进行苦参组培快繁技术体系研究。结果表明,以苦参种子萌发的幼苗为外植体效果较好。3种基本培养基对诱导芽增殖效果差异不显著;在基本培养基中分别加入6-BA、2-ip和ZT诱导芽增殖,但3种细胞分裂素之间差异不显著。培养基中含2 mg/L 6-BA(或ZT或2ip)增殖效果最好,其次是3mg/L的细胞分裂素;以改良MS 2 mg/L 6-BA是苦参较好的增殖培养基;组培苗在1/2 MS 1.5 mg/L IBA培养基上根诱导效果最好(98%),移栽到珍珠岩 蛭石基质(1∶1)中成活率最高(96%)。     

Tissue Culture of Maize I. Callus Induction and Growth

Callus induction and subculture was successful with mature embryos and stem sections of seedlings of Zea mays L. on Linsmaier and Skoog's medium modified to contain 4 mg/I of 2,4-D and 1 g/I of casamino acids. — 2,4-D was superior to NAA and IAA for both callus induction and growth. Callus subcultured on NAA formed abundant roots on agar-solidified media and numerous root-like primordia in liquid cultures. — Kinetin had no effect on callus induction in the presence of 2,4-D and neither kinetin nor gibberellic acid stimulated callus growth during subculture. — Callus grew equally well on the medium of Linsmaier and Skoog, that of Schenk and Hildebrandt, and the B-5 medium of Gamborg and Eveleigh containing 2% sucrose, 4 mg/I of 2,4-D and 1 g/I of casamino acids. — The callus grew more rapidly at 25°C than at 30°C or 35°C. Little difference was noted at any temperature in callus growth in alternating light (16 h) and dark (8 h) or continuous dark. — Sucrose was superior to glucose and maltose in both liquid and agar-solidified cultures. Lactose and galactose failed to support callus growth.     

黄花川西獐牙菜的组织培养

1　植物名称　黄花川西獐牙菜 (Ｓｗｅｒｔｉａｍｕｓｓｏｔｉｉｖａｒ.ｆｌａｖｅｓｃｅｎｓ)。2　材料类别　叶、带节茎段、不带节茎段、顶芽。3　培养条件　基本培养基为ＭＳ培养基。诱导愈伤组织及芽分化培养基 :( 1 )ＭＳ ＮＡＡ 0 .0 6ｍｇ·Ｌ- 1 (单位下同 ) ＩＡＡ 0 .0 6 ＫＴ 0 .1 2 ;( 2 )ＭＳ ＮＡＡ 0 .0 6 ＩＡＡ 0 .0 6 ＫＴ 0 .2 ;( 3)ＭＳ ＮＡＡ0 .0 6 ＩＡＡ 0 .0 6 ＫＴ 0 .3;( 4 )ＭＳ ＮＡＡ 0 .0 6 ＩＡＡ 0 .0 6 ＫＴ 0 .4;( 5 )ＭＳ ＮＡＡ 0 .1 ＩＡＡ 0 .1 ＫＴ 1 .0。生根培养基 :( 6) 1 /2ＭＳ ;( 7) …     

云南红豆杉愈伤组织诱导和组织培养

云南红豆杉愈伤组织的诱导频率为Ｗｈｉｔｅ〉ＭＳ〉Ｂ５,其中最高达８０％,叶片和种子之间差异不大,但不同时期的叶片差异较大,以嫩叶诱导为佳,激素配比以２,４－Ｄ２．０－３．０ｍｇ／Ｌ,ＢＡＰ０．５－１．０ｍｇ／Ｌ为宜,在继代培养阶段,Ｂ５比Ｗｈｉｔｅ和ＭＳ更适合细胞的快速生长、繁殖,并且有利于克服组织的褐化,适合于细胞生长的激素配比也以２,４－Ｄ２．０－３．０ｍｇ／Ｌ,ＢＡＰ０．５－１．０ｍｇ／Ｌｏ     

Effects of Monochromatic Radiations on Growth of Pelargonium Callus Tissue

Pith callus tissues were grown under continuous blue (450 mµ),green (545 mµ), red (650 mµ), and ‘white’(full-spectrum) light, and in the dark for 22 days at 27±2°C at energy levels of 15,000 ergs cm^–2 sec^–1. Mean increases in fresh weight of tissues grown under ‘white’and blue light were significantly greater than those of tissuesgrown in green and red light and in the dark. Tissues grownin the dark yielded mean fresh weight increases significantlylower than tissues grown under blue, red, and ‘white’light. No significant differences were shown between blue and‘white’, red and green, and green and dark treatmentsrespectively. Cell differentiation occurred in all treatmentsonly to the extent of vessel element formation. There were nodifferences in degree of differentiation between treatments. It was proposed that the high-energy reaction of photomorphogenesiswas in operation in the Pelargonium callus tissue. The resultsindicated the presence in the tissue of high-energy photoreceptor(s).The use of high-intensity, incandescent illumination for experimentalprocedures approximating natural conditions of irradiation wasindicated as desirable for pith callus tissues of Pelargoniumzonale var. Enchantress Fiat.     

Purification and Characterization of Sophora flavescens Lectin

A lectin with strong hemagglutination activity was isolated from roots of Sophora flavescens Ait. by extraction, fractionation with (NH 4) 2SO 4, ion-exchange chromatography on DEAE-Sepharose and followed by gel filtration on Sephadex G-150 and HPLC assay. The purified lectin showed a single protein band on PAGE and SDS-PAGE . The molecular weight of S. flavescens lectin was 32 kD when SDS-PAGE and Sephadex G-100 was used. The lectin agglutinated rabbit red blood cells at 0.97 μg/mL and showed no specific agglutination with any type of human erythrocytes. The hemagglutination activity could be inhibited by mannose and levulose and slightly by glucose and maltose. The SFL contained 2.89% neutral saccharide. It could inhibit apparently the growth of the mycelium of Gibberlla saubinetii （Mont.） Sacc.,Piricularia oryzae Cav. and Fusarium vasinfectum Atk. at the dosage of 62 μg. It was determined by Edman that the sequence of the N-terminal thirty amino acids was: T/A/VDXLXFTFSDFDPNGEDLLFQGDAHVTSNN.     

苦参凝集素的分离纯化及部分性质研究

从苦参 (SophoraflavescensAit.)根浸出液经硫酸铵分级 ,得苦参凝集素 (SFL)粗品 ,再经DEAE_Sepharose、SephadexG_1 5 0和HPLC层析 ,获得具有强凝集活性的SFL样品 ,用PAGE和SDS_PAGE检测均为单一蛋白染色带。SDS_PAGE显示SFL分子仅有一条肽链 ,SephadexG_1 0 0和SDS_PAGE测得其分子量均为 32kD。当SFL浓度为 0 .97μg/mL时能凝集兔红细胞 ,无血型专一性 ,其凝血活性可被甘露糖和果糖抑制 ,麦芽糖和葡萄糖有弱的抑制作用 ,凝集素分子含有 2 .89%的中性糖 ;当SFL量为 6 2 μg时 ,对棉花枯萎病菌 (FusariumvasinfectumAtk .)、小麦赤霉病菌(Gibberllasaubinetii (Mont.)Sacc .)和水稻稻瘟病菌 (PiriculariaoryzaeCav)菌丝体的生长发育有明显地抑制作用。用Edman法在蛋白测序仪上测出SFL的N端肽链 30个氨基酸的排列顺序为 :T/A/VDXLXFTFSDFDP NGEDLLFQGDAHVTSNN。     

杉木愈伤组织培养中的褐化控制研究

以杉木茎尖诱导形成的愈伤组织为试验材料,研究维生素C、柠檬酸、半胱氨酸、硫代硫酸钠4种抗氧化剂和活性炭对杉木愈伤组织培养中褐化的影响。结果表明：维生素C和半胱氨酸能有效地控制褐化的发生,且对愈伤组织生长没有明显的抑制作用,是杉木愈伤组织培养较为适宜的抗褐变剂。