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Stress-responsive microRNAs in Populus 总被引:8,自引:0,他引:8
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MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational
inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors
from 11 families and 13 mature miRNAs from 12 families by PCR amplification in P. euphratica. To detect expression differences in mature miRNAs and their precursors under dehydration and high salinity shock in P. euphratica, we examined 14 miRNA precursors from 13 miRNA families and 17 mature miRNAs from 17 miRNA families using the SYBR Green
RT–PCR assay. This is the first report of expression profiles for both precursor and mature miRNAs in P. euphratica. By profiling both the mature miRNAs and the precursors under abiotic stress shock, it was possible to identify miRNA whose
processing is regulated during stress shock environments. A majority of the genes predicted to be targets for plant miRNAs
are involved in development, stress resistance and metabolic processes. We have cloned and experimentally identified in vivo
five of the predicted target genes and quantified the five target mRNAs from the same RNA sample simultaneously. Based on
this study, we propose some regulatory pathways that illustrate the important role that miRNAs play in response to abiotic
stress shock in P. euphratica. 相似文献
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Genome organization and characteristics of soybean microRNAs 总被引:3,自引:0,他引:3
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Genome‐wide identification and in silico characterisation of microRNAs,their targets and processing pathway genes in Phaseolus vulgaris L.
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T. C. de Sousa Cardoso L. G. Portilho C. L. de Oliveira P. C. McKeown W. R. Maluf L. A. A. Gomes T. A. Teixeira L. R. do Amaral C. Spillane M. de Souza Gomes 《Plant biology (Stuttgart, Germany)》2016,18(2):206-219
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Wong CE Zhao YT Wang XJ Croft L Wang ZH Haerizadeh F Mattick JS Singh MB Carroll BJ Bhalla PL 《Journal of experimental botany》2011,62(8):2495-2506
Plant microRNAs (miRNAs) play crucial regulatory roles in various developmental processes. In this study, we characterize the miRNA profile of the shoot apical meristem (SAM) of an important legume crop, soybean, by integrating high-throughput sequencing data with miRNA microarray analysis. A total of 8423 non-redundant sRNAs were obtained from two libraries derived from micro-dissected SAM or mature leaf tissue. Sequence analysis allowed the identification of 32 conserved miRNA families as well as 8 putative novel miRNAs. Subsequent miRNA profiling with microarrays verified the expression of the majority of these conserved and novel miRNAs. It is noteworthy that several miRNAs* were expressed at a level similar to or higher than their corresponding mature miRNAs in SAM or mature leaf, suggesting a possible biological function for the star species. In situ hybridization analysis revealed a distinct spatial localization pattern for a conserved miRNA, miR166, and its star speciessuggesting that they serve different roles in regulating leaf development. Furthermore, localization studies showed that a novel soybean miRNA, miR4422a, was nuclear-localized. This study also indicated a novel expression pattern of miR390 in soybean. Our approach identified potential key regulators and provided vital spatial information towards understanding the regulatory circuits in the SAM of soybean during shoot development. 相似文献
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Chen L Zhang Y Ren Y Xu J Zhang Z Wang Y 《Biochemical and biophysical research communications》2012,417(2):892-896
MicroRNAs (miRNAs) are small, non-coding RNAs that regulate the expression of target mRNAs in plant growth, development, abiotic stress responses, and pathogen responses. Cold stress is one of the most common abiotic factors affecting plants, and it adversely affects plant growth, development, and spatial distribution. To understand the roles of miRNAs under cold stress in Populus tomentosa, we constructed two small RNA libraries from plantlets treated or not with cold conditions (4 °C for 8 h). High-throughput sequencing of the two libraries identified 144 conserved miRNAs belonging to 33 miRNA families and 29 new miRNAs (as well as their corresponding miRNA1s) belonging to 23 miRNA families. Differential expression analysis showed that 21 miRNAs were down-regulated and nine miRNAs were up-regulated in response to cold stress. Among them, 19 cold-responsive miRNAs, two new miRNAs and their corresponding miRNA1s were validated by qRT-PCR. A total of 101 target genes of the new miRNAs were predicted using a bioinformatics approach. These target genes are involved in growth and resistance to various stresses. The results demonstrated that Populus miRNAs play critical roles in the cold stress response. 相似文献