共查询到20条相似文献,搜索用时 31 毫秒
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H.R. Bosshard M. Zürrer H. Schägger G. von Jagow 《Biochemical and biophysical research communications》1979,89(1):250-258
Cytochrome 1, the electron donor for cytochrome , is a subunit of the mitochondrial cytochrome 1 complex (complex III, cytochrome reductase). To test if cytochrome 1 is the cytochrome -binding subunit of the 1 complex, binding of cytochrome to the complex and to isolated cytochrome 1 was compared by a gel-filtration method under non-equilibrium conditions (a 1 complex lacking the Rieske ironsulfur protein was used; von Jagow et al. (1977) Biochim. Biophys. Acta , 549–558). The approximate stoichiometries and binding affinities were found to be very similar. Binding of cytochrome to isolated cytochrome which is another subunit of the reductase was not detectable by the gel-filtration method. Further, the same lysine residues of cytochrome were shielded towards chemical acetylation in the complexes 1 and 1. From this we conclude that the same surface area of cytochrome is in direct contact with cytochrome 1 and with cytochrome 1 in the respective complexes and that therefore cytochrome is most probably the structural ligand for cytochrome in mitochondrial cytochrome reductase. 相似文献
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Maria Szekely Therese Loviny Richard C. Foreman 《Biochemical and biophysical research communications》1976,70(1):110-116
Binding of formylmethionyl tRNA and ribosomes to double stranded RNA has been obtained under conditions identical to those required for initiation complex formation with single stranded RNA. While natural double stranded RNAs from Penicillium virus and Penicillium virus were efficient in forming initiation complexes, the synthetic polynucleotide poly(I).poly (C) was inactive. This suggests that ribosomes can recognize initiation sequences even if these are present in base-paired form. 相似文献
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Y Orii H Shimada T Nozawa M Hatano 《Biochemical and biophysical research communications》1977,76(4):983-988
A possibility of a heme-heme interaction between the heme and heme moieties in nitrite reductase was examined by using magnetic and natural circular dichroism. The MCD of the heme moiety in the ferric enzyme was similar to that of mammalian ferricytochrome in shape and intensity, whereas in the reduced state the MCD intensity was considerably smaller than that of ferrocytochrome . When the heme moiety was perturbed by the complex formation with CO, imidazole or cyanide as well as by pH changes, the depressed MCD was restored to the MCD level of mammalian ferrocytochrome , accompanying conformational changes around the prosthetic groups. Thus, it was concluded that the heme-heme interaction exists only in the reduced enzyme and that this interaction is released under appropriate conditions. 相似文献
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C G Wilde S Boguslawski L L Houston 《Biochemical and biophysical research communications》1979,91(3):1082-1088
The protozoan was found to be resistant to the toxic action of ricin . Isolated ribosomes were strongly resistant to the A subunit of ricin when tested in a cell free protein synthesis system under different conditions and also lacked the ability to bind A chain stoichometrically. This suggests that is resistant because it contains a ribosome which is not susceptible to the toxic action of ricin. 相似文献
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Identification of the structural gene for yeast cytochrome c oxidase subunit I on mitochondrial DNA.
E Keyhani 《Biochemical and biophysical research communications》1979,89(4):1212-1216
Mitochondrial protein synthesis was analyzed in the yeast mit? mutants of which specifically lack cytochrome oxidase. [3H]leucine labeled polypeptides synthesized in yeast OXI 3 mutant were analyzed by means of immunoprecipitation and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). When compared to control, subunit I was not detectable. This result was substantiated by growing OXI 3 mutant in the presence of cycloheximide, an inhibitor of cytoplasmic protein synthesis. Under such conditions SDS-PAGE analysis of [3H]leucine labeled immunoprecipitate shows the absence of subunit I. These data show that the OXI 3 locus contains the structural gene for cytochrome oxidase subunit I. 相似文献
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The formation of cytochrome P-450 “metabolic intermediate” complexes occurred with acetylmethadol and propoxyphene, but not methadone in both naive and phenobarbital-induced animals. The formation correlated with the relative ability of these three compounds to form metabolic intermediate complexes and inhibit mixed-function oxidation reactions . 相似文献
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Material released from rat mast cells by compound gave parallel responses using ACTH and β-endorphin radioimmunoassays. However, incubation of these labeled compounds under conditions of radioimmunoassay with released material and chromatography on Sephadex G-25 provided evidence that neither ACTH nor β-endorphin were present in the material released from mast cells, but represented an artifact produced by the presence of a protease. Analysis of the released enzyme on Sephadex G-75 under non-dissociative conditions yielded an active enzyme complex with a Mr > 150,000. Under dissociative conditions, the Mr of the enzyme was 25,000. The dissociated enzyme reassociated with purified rat mast cell heparin to form the high molecular weight complex. Further investigation of pH, substrate and inhibitor specificity showed that the peptide degradation is due to a chymotrypsin-like protease, the previously described mast cell chymase, which is active in degrading β-endorphin, ACTH, and ACTH1–24. 相似文献
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Juvenile hormones radiobiosynthesised by corpora allata of adult female locusts in vitro 总被引:4,自引:0,他引:4
When corpora allata from adult female are incubated with either 3H-, farnesenic acid or 3H-, , bishomo-farnesenic acid and [-14C] methionine, they fabricate large quantities of the corresponding double labelled methyl 10, 11-epoxy esters. Radio GLC of these products indicates retention of geometric configuration at the C-2 and C-6 double bonds. Separate analyses of the contents of the glands and medium after incubation show that the epoxy esters are rapidly released from the glands into the medium and that only the glands contain the corresponding unepoxidized esters. We suggest that unepoxidized esters are the intracellular intermediates in the formation of juvenile hormones from the unsaturated acids. Gel filtration shows that the epoxy esters are not released as stable protein complexes but as simple solutes into the medium. Using this method of promoting the synthesis of juvenile-hormone-active compounds, rates of biosynthesis of epoxy esters of up to 33 ng. per pair of glands per hour have been achieved. 相似文献
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Effects of cations upon chloroplast membrane subunit interactions and excitation energy distribution
When isolated chloroplasts from mature pea (Pisum sativum) leaves were treated with digitonin under “low salt” conditions, the membranes were extensively solubilized into small subunits (as evidenced by analysis with small pore ultrafilters). From this solubilized preparation, a photochemically inactive chlorophyll · protein complex (chlorophyll ratio, 1.3) was isolated. We suggest that the detergent-derived membrane fragment from mature membranes is a structural complex within the membrane which contains the light-harvesting chlorophyll protein and which acts as a light-harvesting antenna primarily for Photosystem II.Cations dramatically alter the structural interaction of the light-harvesting complex with the photochemically active system II complex. This interaction has been measured by determining the amount of protein-bound chlorophyll b and Photosystem II activity which can be released into dispersed subunits by digitonin treatment of chloroplast lamellae. When cations are present to cause interaction between the Photosystem II complex and the light-harvesting pigment · protein, the combined complexes pellet as a “heavy” membranous fraction during differential centrifugation of detergent treated lamellae. In the absence of cations, the two complexes dissociate and can be isolated in a “light” submembrane preparation from which the light-harvesting complex can be purified by sucrose gradient centrifugation.Cation effects on excitation energy distribution between Photosystems I and II have been monitored by following Photosystem II fluorescence changes under chloroplast incubation conditions identical to those used for detergent treatment (with the exception of chlorophyll concentration differences and omission of detergents). The cation dependency of the pigment · protein complex and Photosystem II reaction center interactions measured by detergent fractionation, and regulation of excitation energy distribution as measured by fluorescence changes, were identical. We conclude that changes in substructural organization of intact membranes, involving cation induced changes in the interaction of intramembranous subunits, are the primary factors regulating the distribution of excitation energy between Photosystems II and I. 相似文献
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RNA-protein interactions in the ribosome. I. Characterization and ribonuclease digestion of 16 S RNA-ribosomal protein complexes 总被引:14,自引:0,他引:14
Proteins from the 30 S ribosomal subunit of Escherichia coli were fractionated by column chromatography and individually incubated with 16 S ribosomal RNA. Stable and specific complexes were formed between proteins S4, S7, S8, S15 and S20, and the 16 S RNA. Protein S13 and one or both proteins of the mixture bound more weakly to the RNA, although these interactions too were apparently specific. The binding of was found to be markedly stimulated by proteins S4, S8, S15 and S20. Limited digestion of the RNA-protein complexes with T1 or pancreatic ribonucleases yielded a variety of partially overlapping RNA fragments, which retained one or more of the proteins. Since similar fragments were recovered when 16 S RNA alone was digested under the same conditions, their stability could not be accounted for by the presence of bound protein. The integrity of the fragments was, however, strongly influenced by the magnesium ion concentration at which ribonuclease digestion was carried out. Each of the RNA fragments was characterized by fingerprinting and positioned within the sequence of the 1600-nucleotide 16 S RNA molecule. The location of ribosomal protein binding sites was delimited by the pattern of fragments to which a given protein bound. The binding sites for proteins S4, S8, S15, S20 and, possibly, S13 and as well, lie within the 5′-terminal half of the 16 S RNA molecule. In particular, the S4 binding site was localized to the first 500 nucleotides of this sequence while that for S15 lies within a 140-nucleotide sequence starting about 600 nucleotides from the 5′-terminus. The binding site for the protein S7 lies between 900 and 1500 nucleotides from the 5′-terminus of the ribosomal RNA. 相似文献
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A manganese-stimulated endonuclease from Bacillus subtilis 总被引:6,自引:0,他引:6
An endonuclease activity has been identified in extracts of . This activity is stimulated by Mn++ or Ca++ ions but not by Mg++ ions. The enzyme catalyzes the breakdown of native DNA of high molecular weight to fragments of molecular weights ranging from 3 × 106 to 20 × 106. A variety of DNA's from sources such as and T7 phage are attacked. About 61% of the activity of the cells is released into the medium during protoplast formation under conditions where 98% of the glucose 6-P dehydrogenase activity is retained by the cells. 相似文献
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J Sy N H Chua Y Ogawa F Lipmann 《Biochemical and biophysical research communications》1974,56(3):611-616
Ribosomes obtained from (ATCC 8185) were slightly active in synthesizing guanosine polyphosphates, which activity was greatly stimulated by addition of stringent factor. chloroplast ribosomes did not produce guanosine polyphosphates on incubation but responded with abundant synthesis to addition of the stringent factor from . In contrast, cytoplasmic ribosomes from the same organism did not respond. Interchange experiments between either subunit from chloroplasts with the counterparts showed good activity. When the small subunit of cytoplasmic ribosomes was combined with the large subunit of or of chloroplasts, a small but definite response was obtained. 相似文献