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1.
A polyphasic taxonomic study was carried out on strains PB105 T and PB108 isolated from a grass soil in Korea. The cells of the strains were Gram-stain negative, non-spore-forming, non-motile, and rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of these strains with Bacteroidetes, which showed high pairwise sequence similarities with Hymenobacter algoricola VUG-A23a T (99.2%), Hymenobacter fastidiosus VUG-A124a T (97.4%), and Hymenobacter daecheongensis Dae14 T (96.9%). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strains formed a clear phylogenetic lineage with the genus Hymenobacter. The major fatty acids were identified as C 15:0 iso, C 15:0 anteiso, C 16:1 ω5 c, C 15:0 iso 3-OH, C 17:0 iso 3-OH, summed feature 3 (C 16:1 ω6 c and/or C 16:1 ω7 c/ t), and summed feature 4 (C 17:1 anteiso B and/or C 17:1 iso I). The major cellular polar lipids were identified as phosphatidylethanolamine, an unidentified aminolipid, and two unidentified lipids. The respiratory quinone was identified as MK-7 and the genomic DNA G+C content was determined to be 64.5 mol% for strain PB105 T and 64.1 mol% for strain PB108. DNA–DNA hybridization value of type strain PB105 T with H. algoricola VUG-A23a T was 32.3% (reciprocal 39.2). Based on the combined genotypic and phenotypic data, we propose that strains PB105 T and PB108 represent a novel species of the genus Hymenobacter, for which the name Hymenobacter daejeonensis sp. nov. is proposed. The type strain is PB105 T (=?KCTC 52579 T?=?JCM 31885 T). 相似文献
2.
A bacterial isolate was recovered from a soil sample collected in Jeollabuk-do Province, South Korea, and subjected to polyphasic taxonomic assessment. Cells of the isolate, designated strain S1-2-1-2-1 T, were observed to be rod-shaped, pink in color, and Gram-stain negative. The strain was able to grow at temperature range from 10 to 30 °C, with an optimum of 25 °C, and growth occurred at pH 6–8. Comparative 16S rRNA gene sequence analysis showed that strain S1-2-1-2-1 T belongs to the genus Hymenobacter, with closely related type strains being Hymenobacter daeguensis 16F3Y-2 T (95.8% similarity), Hymenobacter rubidus DG7B T (95.8%), Hymenobacter soli PB T (95.7%), Hymenobacter terrenus MIMtkLc17 T (95.6%), Hymenobacter terrae DG7A T (95.3%), and Hymenobacter saemangeumensis GSR0100 T (95.2%). The genomic DNA G+C content of strain S1-2-1-2-1 T was 63.0 mol%. The main polar lipid of this strain was phosphatidylethanolamine, the predominant respiratory quinone was menaquinone-7, and the major fatty acids were C 15:0 iso (27.3%), summed feature 3 (C 16:1 ω7 c/C 16:1 ω6 c) (16.5%), C 15:0 anteiso (15.3%), and C 16:0 (14.7%), supporting the affiliation of this strain with the genus Hymenobacter. The results of this polyphasic analysis allowed for the genotypic and phenotypic differentiation of strain S1-2-1-2-1 T from recognized Hymenobacter species. On the basis of its phenotypic properties, genotypic distinctiveness, and chemotaxonomic features, strain S1-2-1-2-1 T is considered to represent a novel species of the genus Hymenobacter, for which the name Hymenobacter agri sp. nov. is proposed. The type strain is S1-2-1-2-1 T (=KCTC 52739 T?=?JCM 32194 T). 相似文献
3.
A group of thirteen bacterial strains was isolated from rock samples collected in a deglaciated northern part of James Ross Island, Antarctica. The cells were rod-shaped, Gram-stain-negative, non-motile, catalase positive, and produced moderately slimy, ultraviolet light (UVC)-irradiation-resistant and red–pink pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, extensive biotyping, fatty acid profile, chemotaxonomy analyses, and whole genome sequencing were applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all isolates constituted a coherent group belonging to the genus Hymenobacter. The closest relatives to the representative isolate P5136 T were Hymenobacter psychrophilus BZ33r T and Hymenobacter rubripertinctus CCM 8852 T, exhibiting 97.53% and 97.47% 16S rRNA pairwise similarity, respectively. Average nucleotide identity calculated from the whole-genome sequencing data supported the finding that P5136 T represents a distinct Hymenobacter species. The major components in fatty acid profiles were Summed Feature 3 (C 16:1 ω7c/C 16:1 ω6c), C 16:1 ω5 c, C 15:0 iso and C 15:0 anteiso. The cellular quinone content contained unanimously menaquinone MK-6 and MK-7 (ratio 1:5.1). The predominant polar lipid was phosphatidylethanolamine, and moderate to minor amounts of two unknown polar lipids, two unknown aminolipids, one unknown glycolipid and two unknown glycophospholipids were present. The G + C content of genomic DNAs is 60.31 mol%. Based on all the obtained results, we propose a novel species for which the name Hymenobacter amundsenii sp. nov. is suggested, with the type strain P5136 T (= CCM 8682 T = LMG 29687 T). 相似文献
4.
A Gram-stain-negative, non-motile, non-spore-forming, rodshaped, aerobic bacterial strain, designated S1-2-2-5 T, was isolated from the Jeollabuk-do province, Republic of Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain S1-2-2-5 T belonged to the family Cytophagaceae in phylum Bacteroidetes, and was most closely related to Hymenobacter terrae DG7A T (98.2%), Hymenobacter rubidus DG7B T (98.0%), Hymenobacter soli PB17 T (97.7%), Hymenobacter daeguensis 16F3Y-2 T (97.2%) and Hymenobacter saemangeumensis GSR0100 T (97.0%). The G + C content of the genomic DNA of strain S1-2-2-5 T was 59.4 mol%. The detection of menaquinone MK-7 as the predominant respiratory quinone, a fatty acid profile with summed feature 3 (C 16:1ω7 c/C 16:1ω6 c; 32.0%), C 15:0 iso (19.0%), and C 15:0 anteiso (15.0%) as the major components, and a polar lipid profile with phosphatidylethanolamine as the major component supported the affiliation of strain S1-2-2-5 T to the genus Hymenobacter. The DNA-DNA relatedness between strain S1-2-2-5 T and H. terrae KCTC 32554 T, H. rubidus KCTC 32553 T, H. soli KCTC 12607 T, H. daeguensis KCTC 52537 T, and H. saemangeumensis KACC 16452 T were 49.5, 48.2, 34.1, 28.1, and 31.8% respectively, clearly showing that the isolate is not related to them at the species level. Strain S1-2-2-5 T could be clearly differentiated from its closest neighbors on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain S1-2-2-5 T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter terrigena sp. nov. is proposed. The type strain is S1-2-2-5 T (= KCTC 52737 T = JCM 32195 T). 相似文献
5.
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterial strain, designated 16F3Y-2 T, was isolated from the Han River, South Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 16F3Y-2 T belonged to the family Cytophagaceae in the phylum Bacteroidetes and was most closely related to ‘ Hymenobacter terrae’ DG7A (98.01%), H. soli PB17 T (97.26%), H. glaciei VUG-A130 T (96.78%), H. antarcticus VUG-A42aa T (96.72%), H. ruber PB156 T (96.61%), and H. saemangeumensis GSR0100 T (95.77%). The G+C content of the genomic DNA of strain 16F3Y-2 T was 62.9 mol%. The isolate contained MK-7 as the predominant respiratory quinone, and summed feature 3 (C 16:1 ω7 c/C 16:1 ω6 c; 35.5%), C 15:0 iso (16.9%), C 16:1 ω5 c (10.9%), and C 15:0 anteiso (9.9%) as major fatty acids. The major polar lipid was phosphatidylethanolamine. Phenotypic and chemotaxonomic data supported the affiliation of strain 16F3Y-2 T with the genus Hymenobacter. However, strain 16F3Y-2 T exhibited relatively low levels of DNA-DNA relatedness with ‘ H. terrae’ KCTC 32554 (44.1%) and H. soli KCTC 12607 T (24.3%), clearly indicating that the isolate constitutes a new genospecies. Strain 16F3Y-2 T could be differentiated from its phylogenetic neighbors on the basis of several phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 16F3Y-2 T represents a novel species in the genus Hymenobacter, for which the name Hymenobacter daeguensis sp. nov. is proposed. The type strain is 16F3Y-2 T (=KCTC 52537 T =JCM 31654 T). 相似文献
6.
A white-coloured bacterium, designated strain GTJR-20 T, was isolated from a stem of Phytolacca acinosa Roxb. collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. The novel isolate was found to grow optimally at 28–30 °C, at pH 7.5–8.0 and in the absence of NaCl. Cells were observed to be Gram-stain positive, strictly aerobic, rod-shaped and non-motile. The predominant respiratory quinone was identified as MK-7(H 4) and the major cellular fatty acids were identified as iso-C 16:0 (35.8 %), C 18:1 ω9 c (17.7 %), C 17:1 ω6 c (11.0 %), C 17:1 ω8 c (7.8 %) and C 18:3 ω6 c (6, 9, 12) (7.2 %). The DNA G+C content was determined to be 71.6 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTJR-20 T is a member of the genus Solirubrobacter and is closely related to Solirubrobacter phytolaccae GTGR-8 T (16S rRNA gene sequence similarity, 98.4 %), Solirubrobacter soli KCTC 12628 T (97.8 %), Solirubrobacter pauli KCTC 9974 T (97.7 %) and Solirubrobacter ginsenosidimutans KCTC 19420 T (97.6 %). No other recognized bacterial species showed more than 94.6 % 16S rRNA gene sequence similarity to the novel isolate. DNA–DNA relatedness values for strain GTJR-20 T with respect to its closely related neighbours S. phytolaccae GTGR-8 T, S. soli KCTC 12628 T, S. pauli KCTC 9974 T and S. ginsenosidimutans KCTC 19420 T were 48.3 ± 8.6, 21.3 ± 5.2, 36.8 ± 6.2 and 36.0 ± 5.5 %, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain GTJR-20 T is considered to represent a novel species of the genus Solirubrobacter, for which the name Solirubrobacter taibaiensis sp. nov. is proposed. The type strain is GTJR-20 T (=CCTCC AB 2013308 T = KCTC 29222 T). 相似文献
7.
A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterial strain, designated 1-3-3-8 T, was isolated from soil and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 1-3-3-8 T belongs to the family Cytophagaceae of phylum Bacteroidetes and is most closely related to Hymenobacter paludis KBP-30 T (96.8% similarity), Hymenobacter ocellatus Myx2105 T (96.8%), Hymenobacter coalescens WW84 T (95.6%), and Hymenobacter deserti ZLB-3 T (95.4%). The G + C content of the genomic DNA of strain 1-3-3-8 T was 63.6 mol%. The isolate contained C 15:0 iso (28.4%), summed feature 4 (C 17:1 anteiso B/C 17:1 iso I; 18.9%), and C 15:0 anteiso (17.6%) as major fatty acids, MK-7 as the predominant respiratory quinone, and sym-homospermidine as the predominant polyamine. The major polar lipids were phosphatidylethanolamine and an unidentified lipid. The phenotypic and chemotaxonomic data supported the affiliation of strain 1-3-3-8 T with the genus Hymenobacter. The DNA-DNA relatedness between strain 1-3-3-8 T and H. paludis KCTC 32237 T and H. ocellatus DSM 11117 T were 24.5 and 27.4% respectively, clearly showing that the isolate is not related to them at the species level. Overall, the novel strain could be differentiated from its phylogenetic neighbors on the basis of several phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 1-3-3-8 T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter jeollabukensis sp. nov. has been proposed. The type strain is 1-3-3-8 T (= KCTC 52741 T = JCM 32192 T). 相似文献
8.
A novel Gram-negative and red-pinkish bacterium designated DG5B T was isolated from a dry soil. Cells were rods that were catalase- and oxidase-positive, and non-motile. The strain was found to grow at temperatures from 10 to 30°C (optimum 25°C) and pH 6.0–8.0, (optimum pH 7) on R2A broth. 16S rRNA gene sequence (1,452 bp) analysis of this strain identified it as a member of the genus Hymenobacter that belongs to the class Cytophagia. The highest gene sequence similarities were with Hymenobacter arizonensis OR362-8 T (98.3%), Hymenobacter humi DG31A T (97.6%), and Hymenobacter glaciei VUG-A130 T (96.6%). Strain DG5B T exhibited <70% DNA-DNA relatedness with H. arizonensis (34.7 ± 7.0%; reciprocally, 29.7 ± 1.2%) and H. humi (39.4 ± 4.3%; reciprocally, 39.5 ± 3.3%) as a different genomic species, and its genomic DNA G+C content was 59.8%. Strain DG5B T had the following chemotaxonomic characteristics: the major fatty acids are iso-C 15:0, anteiso-C 15:0, C 16:1ω5 c, and summed feature 3 (C 16:1ω7 c / C 16:1ω6 c); polar lipid profile contained phosphatidylethanolamine (PE), unknown aminophospholipid (APL), unknown glycolipids (GL), unknown phospholipids (PL), and unknown polar lipids (L); the major quinone is MK-7. The absorbance peak of pigment is at 481.0 nm. Strain DG5B T showed low-level resistance to gamma-ray irradiation. Phenotypic, chemotaxonomic, and genotypic properties indicated that isolate DG5B T represents a novel species within the genus Hymenobacter for which the name Hymenobacter sedentarius sp. nov. is proposed. The type strain is DG5B T (=KCTC 32524 T =JCM 19636 T). 相似文献
9.
Strain OR362-8 T was isolated from a biological soil crust sample collected from the southwestern arid lands of the United States of America, using BG11-PGY medium. Cells of OR362-8 T were found to be rod shaped; occur singly, as pairs and in groups; non-motile; positive for catalase, oxidase, phosphatase and gelatinase; hydrolyze starch; contain iso-C 15:0, anteiso-C 15:0, iso-C 15:1G, C 16:1ω5c and summed feature 3 (C 16:1(ω7c)/iso-C 15:0 2OH as defined by the MIDI system) as the major fatty acids; and MK-7 as the sole respiratory quinone. A BLAST sequence similarity search using 16S rRNA gene sequence of OR362-8 T identified Hymenobacter as the nearest genus with a similarity of 90.4–96.9 %. The phylogenetic analyses based on the phenetic methods UPGMA, NJ, ME and DNA parsimony resulted in the clustering of OR362-8 T with Clade 1 Hymenobacter species represented by Hymenobacter glaciei, Hymenobacter antarcticus, Hymenobacter flocculans, Hymenobacter metalli and Hymenobacter soli with the closest being the Hymenobacter glaciei (96.9 % 16S rRNA gene sequence similarity). Besides the strong phylogentic affiliation, OR362-8 T also exhibited significant phenotypic and chemotaxonomic differences with the members of Clade 1 Hymenobacter spp. More importantly, the DNA G+C content (mol%) of OR362-8 T is very high (70 %) compared to the nearest species identified by phylogenetic analysis. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, OR362-8 T was assigned to a novel species for which we propose here the name Hymenobacter arizonensis sp. nov., with OR362-8 T (=ATCC BAA 1266 T = DSM 17860 T = JCM 13504 T) as the type strain. 相似文献
10.
Strain DY59 T, a Gram-positive non-motile bacterium, was isolated from soil in South Korea, and was characterized to determine its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence of strain DY59 T revealed that the strain DY59 T belonged to the family Deinococcaceae in the class Deinococci. The highest degree of sequence similarities of strain DY59 T were found with Deinococcus radiopugnans KACC 11999 T (99.0%), Deinococcus marmoris KACC 12218 T (97.9%), Deinococcus saxicola KACC 12240 T (97.0%), Deinococcus aerolatus KACC 12745 T (96.2%), and Deinococcus frigens KACC 12220 T (96.1%). Chemotaxonomic data revealed that the predominant fatty acids were iso-C 15:0 (19.0%), C 16:1 ω7 c (17.7%), C 15:1 ω6 c (12.6%), iso-C 17:0 (10.3%), and iso-C 17:1 ω9 c (10.3%). A complex polar lipid profile consisted of a major unknown phosphoglycolipid. The predominant respiratory quinone is MK-8. The cell wall peptidoglycan contained D-alanine, L-glutamic acid, glycine, and L-ornithine (di-amino acid). The novel strain showed resistance to gamma radiation, with a D 10 value (i.e. the dose required to reduce the bacterial population by 10-fold) in excess of 5 kGy. Based on the phylogenetic, chemotaxonomic, and phenotypic data, strain DY59 T (=KCTC 33033 T =JCM 18581 T) should be classified as a type strain of a novel species, for which the name Deinococcus swuensis sp. nov. is proposed. 相似文献
11.
A Gram-negative, aerobic, non-motile, dark brown-coloured and rod-shaped bacterial strain, designated G-MB1 T, was isolated from a tidal flat sediment of the South Sea, South Korea. Strain G-MB1 T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain G-MB1 T fell within the clade comprising Thalassomonas species, clustering with the type strains of Thalassomonas agarivorans, Thalassomonas loyana, Thalassomonas ganghwensis and Thalassomonas agariperforans, with which it exhibited 16S rRNA gene sequence similarity values of 96.0–96.9 %. The 16S rRNA gene sequence similarity values between strain G-MB1 T and the type strains of the other Thalassomonas species were 94.6–95.1 %. Strain G-MB1 T was found to contain Q-8 as the predominant ubiquinone and C 16:0, C 17:1 ω8 c, C 16:1 ω9 c, C 12:0 3-OH and summed feature 3 (C 16:1 ω7 c and/or C 16:1 ω6 c) as the major fatty acids. The major polar lipids of strain G-MB1 T were phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. The DNA G+C content of strain G-MB1 T was determined to be 42.4 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain G-MB1 T is separated from other Thalassomonas species. On the basis of the data presented, strain G-MB1 T is considered to represent a novel species of the genus Thalassomonas, for which the name Thalassomonas fusca sp. nov. is proposed. The type strain is G-MB1 T (=KCTC 32499 T = NBRC 109830 T). 相似文献
12.
A Gram-negative, aerobic, motile rod strain, designated Ma-20 T, was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China, and was subjected to a polyphasic taxonomy study. Strain Ma-20 T can grow in the presence of 0.5–11 % (w/v) NaCl, 10–43 °C and pH 6–10, and grew optimally at 30 °C, pH 7.5–9.0 in natural seawater medium. The polar lipids were composed of phosphatidylethanolamine, three unidentified phospholipids and three unidentified polar lipids. The respiratory quinone was ubiquinone 8 (Q-8) and the major fatty acids were C 18:1ω6c/C 18:1ω7c (summed feature 8, 32.84 %), C 16:1ω6c/C 16:1ω7c (summed feature 3, 30.76 %), C 16:0 (13.54 %), C 12:03-OH (4.63 %), and C 12:0 (4.09 %). The DNA G+C content of strain Ma-20 T was 58 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ma-20 T belonging to Gammaproteobacteria, it shared 88.46–91.55 and 89.21–91.26 % 16S rRNA gene sequence similarity to the type strains in genus Hahella and Marinobacter, respectively. In addition to the large 16S rRNA gene sequence difference, Ma-20 T can also be distinguished from the reference type strains Hahella ganghwensis FR1050 T and Marinobacter hydrocarbonoclasticus sp. 17 T by several phenotypic characteristics and chemotaxonomic properties. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain Ma-20 T is suggested to represent a novel species of a new genus in Gammaproteobacteria, for which the name Nonhongiella spirulinensis gen. nov., sp. nov. is proposed. The type strain is Ma-20 T (=KCTC 32221 T=LMG 27470 T). 相似文献
13.
A Gram-staining-negative, rod-shaped and motile with several polar flagellums bacterium, designated WM-3 T, was isolated from a rice paddy soil in South China. Growth occurred with 0–3.0 % (w/v) NaCl (optimum 2.0 %), at pH 5.5–9.0 (optimum pH 7.0) and at 25–42 °C (optimum 30–37 °C) in liquid Reasoner’s 2A medium. Analysis of the 16S rRNA gene and gyrB gene sequences revealed that strain WM-3 T was most closely related to the type strains of the species Pseudomonas linyingensis and Pseudomonas sagittaria. Its sequence similarities with P. linyingensis CGMCC 1.10701 T and P. sagittaria JCM 18195 T were 97.4 and 97.3 %, respectively, for 16S rRNA gene, and were 94.1 and 94.2 %, respectively, for gyrB gene. DNA–DNA hybridization between strain WM-3 T and these two type strains showed relatedness of 35.6 and 30.9 %, respectively. G+C content of genomic DNA was 69.4 mol%. The whole-cell fatty acids mainly consisted of C 16:0 (30.0 %), C 16:1 ω6c and/or C 16:1 ω7c (19.3 %) and C 18:1 ω6 c and/or C 18:1 ω7 c (16.3 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain WM-3 T belongs to genus Pseudomonas but represents a novel species, for which the name Pseudomonas oryzae sp. nov. is proposed. The type strain is WM-3 T (=KCTC 32247 T =CGMCC 1.12417 T). 相似文献
14.
Two novel Gram-negative bacterial strains BT442T and BT584 were isolated from dry soil collected in mountains Busan and Guri, Korea during wintertime. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT442T and BT584 both belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia). Strain BT442T was closely related to Hymenobacter soli PB17T (98.0% 16S rRNA gene similarity) and Hymenobacter terrae POA9T (97.6%). No other recognized bacterial species showed more than 97% 16S rRNA gene sequence similarity to strains BT442T. The genome size of strain BT442T was 5,143,362 bp. Bacterial growth was observed at 10–30 °C (optimum 25 °C), pH 6.0–8.0 (optimum pH 6.0) in R2A agar and in the presence up to 1% NaCl. The major cellular fatty acids of strains BT442T and BT584 were iso-C15:0, anteiso-C15:0 and summed feature 3 (C16:1 ω6c / C16:1 ω7c). In addition, their predominant respiratory quinone was MK-7. The major polar lipids of strains BT442T and BT584 were identified to be phosphatidylethanolamine, aminophospholipid, and aminolipid. Based on the biochemical, chemotaxonomic, and phylogenetic analyses, strains BT442T and BT584 are novel bacterial species within the genus Hymenobacter, and the proposed name is Hymenobacter negativus. The strain type of Hymenobacter negativus is BT442T (=?KCTC 72902T?=?NBRC XXXXT). 相似文献
15.
A Gram-negative, non-spore-forming, aerobic, motile and rod-shaped or ovoid bacterial strain, designated MA1-3 T, was isolated from a sea squirt ( Halocynthia roretzi) collected from the South sea in South Korea. Strain MA1-3 T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain MA1-3 T fell within the clade comprising Colwellia species, clustering coherently with the type strains of Colwellia aestuarii, Colwellia polaris and Colwellia chukchiensis, showing sequence similarity values of 97.2, 96.4 and 95.6 %, respectively. It exhibited 16S rRNA gene sequence similarity values of 93.9–96.1 % to the type strains of the other Colwellia species. Strain MA1-3 T was found to contain Q-8 as the predominant ubiquinone and C 16:1 ω7 c and/or C 16:1 ω6 c, C 16:0 and C 16:1 ω9 c as the major fatty acids. The DNA G+C content of strain MA1-3 T was determined to be 39.1 mol% and its mean DNA–DNA relatedness value with the type strain of C. aestuarii was 13 ± 5.4 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separated from other Colwellia species. On the basis of the data presented, strain MA1-3 T is considered to represent a novel species of the genus Colwellia, for which the name Colwellia meonggei sp. nov. is proposed. The type strain is MA1-3 T (=KCTC 32380 T = CECT 8302 T). 相似文献
16.
A Gram-stain negative, aerobic, motile and rod-shaped bacterial strain, designated J-MY2 T, was isolated from a tidal flat sediment of the South Sea, South Korea. Strain J-MY2 T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain J-MY2 T forms a cluster with the type strains of Simiduia species. Strain J-MY2 T exhibited 16S rRNA gene sequence similarity values of 97.62–98.77 % to the type strains of four Simiduia species and of <92.95 % sequence similarity to the type strains of the other recognized species. Strain J-MY2 T was found to contain Q-8 as the predominant ubiquinone and summed feature 3 (C 16:1 ω7 c and/or C 16:1 ω6 c), C 16:0, C 18:1 ω7 c and C 17:1 ω8 c as the major fatty acids. The major polar lipids of strain J-MY2 T were identified as phosphatidylethanolamine, phosphatidylglycerol, three unidentified glycolipids and one unidentified lipid. The DNA G+C content of strain J-MY2 T was determined to be 54.8 mol% and its mean DNA–DNA relatedness values with the type strains of the four Simiduia species were in the range 21–34 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain J-MY2 T is separated from other Simiduia species. On the basis of the data presented, strain J-MY2 T is considered to represent a novel species of the genus Simiduia, for which the name Simiduia aestuariiviva sp. nov. is proposed. The type strain is J-MY2 T ( = KCTC 42073 T = CECT 8571 T). 相似文献
17.
A Gram stain -negative, strictly aerobic, rod-shaped, non-motile and deep-yellow-coloured bacterial strain, designated ZFJR-3 T, was isolated from the stem of Geum aleppicum Jacq. collected from Taibai Mountain in Shaanxi Province, north-west China, and characterized by using a polyphasic approach. The novel isolate grew optimally at 25–28 °C and in the absence of NaCl. Flexirubin-type pigments were produced. The predominant respiratory quinone was ubiquinone-8 (Q-8) and the major cellular fatty acids were iso-C 15:0 (29.2 %), iso-C 16:0 (18.5 %), summed feature 9 (comprising iso-C 17:1 ω9 c and/or C 16:0 10-methyl; 8.8 %), C 16:1 ω7 c alcohol (8.8 %), iso-C 11:0 3-OH (6.9 %) and iso-C 11:0 (6.8 %). The DNA G+C content was 66.1 mol %. The only polyamine was spermidine and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZFJR-3 T belongs to the genus Pseudoxanthomonas and was most closely related to Pseudoxanthomonas yeongjuensis KCTC 22757 T (16S rRNA gene sequence similarity, 99.0 %) and Pseudoxanthomonas sacheonensis KCTC 22080 T (98.0 %). The levels of 16S rRNA gene sequence similarity with respect to other Pseudoxanthomonas species with validly published names were less than 96.5 %. DNA–DNA relatedness values for strain ZFJR-3 T with respect to its closely related neighbours P. yeongjuensis KCTC 22757 T and P. sacheonensis KCTC 22080 T were 48.7 and 36.3 %, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain ZFJR-3 T is considered to represent a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas gei sp. nov. is proposed. The type strain is ZFJR-3 T (=CCTCC AB 2013020 T =KCTC 32298 T). 相似文献
18.
A Gram stain-negative, aerobic and rod-shaped bacterium, strain DY22 T, was isolated from a deep-sea sediment collected from the east Pacific Ocean. The isolate was found to grow in the presence of 0–20.0 % (w/v) NaCl and at pH 4.5–8.5; optimum growth was observed with 0.5–2.0 % (w/v) NaCl and at pH 5.0–7.0. Chemotaxonomic analysis showed the presence of ubiquinone-9 as predominant respiratory quinone and C 16:0, C 19:0 ω8 c cyclo and C 12:0 3-OH as major cellular fatty acids. The genomic DNA G+C content was determined to be 59.6 mol%. Comparative 16S rRNA gene sequence analysis revealed that the novel isolate belongs to the genus Salinicola. Strain DY22 T exhibited the closest phylogenetic affinity to the type strain of Salinicola salarius with 97.2 % sequence similarity and less than 97 % sequence similarity with respect to other Salinicola species with validly published names. The DNA–DNA reassociation values between strain DY22 T and S. salarius DSM 18044 T was 52 ± 4 %. On the basis of phenotypic, chemotaxonomic and genotypic data, strain DY22 T represents a novel species of the genus Salinicola, for which the name Salinicola peritrichatus sp. nov. (type strain DY22 T = CGMCC 1.12381 T = JCM 18795 T) is proposed. 相似文献
19.
Two moderately halophilic, facultatively aerobic, motile bacteria with flagella, designated strains 10-C-3 T and 30-C-3, were isolated from jeotgal, a traditional Korean fermented seafood. Cells of the strains were observed to be ovoid-rods showing catalase- and oxidase-positive reactions and production of creamy-pink pigments. Growth of strain 10-C-3 T was observed at 15–35 °C (optimum, 25–30 °C), at pH 5.5–9.0 (optimum, pH 7.0–7.5), and in the presence of 3–15 % (w/v) salts (optimum: 5–10 %). The two strains were found to contain C 18:1 ω7 c, C 16:0, summed feature 3 (as defined by the MIDI system, comprising C 16:1 ω7 c and/or C 16:1 ω6 c), and C 12:0 3-OH as the major cellular fatty acids. The G+C contents of the genomic DNA of strains 10-C-3 T and 30-C-3 were determined to be 63.2 and 63.1 mol%, respectively and the respiratory quinone detected was ubiquinone 9 (Q-9) only. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 10-C-3 Tand 30-C-3 formed a distinct phyletic lineage within the genus Halomonas and are most closely related to Halomonas fontilapidosi 5CR T with 95.2 % of 16S rRNA sequence similarity. Strains 10-C-3 Tand 30-C-3 shared 99.2 % of 16S rRNA gene sequence similarity and their DNA–DNA relatedness value was 96.6 ± 0.9 %. On the basis of phenotypic, chemotaxonomic and molecular features, strains 10-C-3 Tand 30-C-3 represent a novel species of the genus Halomonas, for which the name Halomonas cibimaris sp. nov. is proposed. The type strain is 10-C-3 T (= KACC 14932 T = JCM 16914 T). 相似文献
20.
A red–pink, Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain DK6-37 was isolated from the permafrost region of Qilian Mountains in northwest of China. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that this isolate represents a novel member of the genus Hymenobacter, with low sequence similarities (<97 %) to recognized Hymenobacter species. Optimum growth was observed at 28 °C, pH 7.0 and 0 % NaCl. The strain was found to contain MK-7 as the predominant menaquinone. The polar lipids were identified as phosphatidylethanolanmine, two unknown aminophospholipids, one unknown aminolipid and three unknown polar lipids. The major fatty acids were identified as summed feature 3 (C 16:1 ω7 c/C 16:1 ω6 c as defined by MIDI), summed feature 4 (anteiso-C 17:1 B/iso-C 17:1 I), C 16:1 ω5 c, iso-C 17:0 3-OH, iso-C 15:0 and C 18:0. The DNA G + C content was determined to be 67.4 mol %. On the basis of the polyphasic evidence presented, it is proposed that strain DK6-37 represents a novel species of the genus Hymenobacter, for which the name Hymenobacter qilianensis sp. nov. is proposed. The type strain is DK6-37 T (= CGMCC 1.12720 T = JCM 19763 T). 相似文献
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