共查询到20条相似文献,搜索用时 31 毫秒
1.
Kangping Wang Hengmin Cui Yuanxin Deng Xi Peng Zhicai Zuo Jing Fang Junliang Deng Wei Cui Bangyuan Wu 《Biological trace element research》2012,149(2):212-218
The purpose of this 42-day study was to examine the effect of dietary vanadium on intestinal microorganism diversity in the duodenum, ileum, cecum, and rectum segments of broilers by the plate count and polymerase chain reaction?Cdenaturing gradient gel electrophoresis (DGGE). A total of 420 1-day-old avian broilers were divided into six groups and fed on a control diet or the same diet supplemented with vanadium at the doses of 5, 15, 30, 45, and 60?mg/kg in the form of ammonium metavanadate. In comparison with control group, the dietary vanadium at the doses of 45 and 60?mg/kg could decrease the counts of Bifidobacterium spp. in the intestinal tract at 21 and 42?days of age. With increasing level in dietary vanadium, the counts of Escherichia coli were significantly increased in the ileum, cecum, and rectum and were decreased in the duodenum at 21 and 42?days of age. However, the counts of Lactobacilli were decreased in the cecum and rectum and increased in the ileum of 45 and 60?mg/kg groups. The colonization of these three bacteria could be affected by dietary vanadium. DGGE analysis showed that the number of bands in duodenum, ileum, cecum, and rectum were obviously decreased in the 30, 45, and 60?mg/kg groups at 21 and 42?days of age. In conclusion, the dietary vanadium in excess of 30?mg/kg could alter the amount and diversity of intestinal bacteria in broilers, implying that the structure and initial balance in the intestinal microbiota were disrupted. 相似文献
2.
Deng Y Cui H Peng X Fang J Wang K Cui W Liu X 《Biological trace element research》2011,144(1-3):647-656
The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on intestinal immune function by histopathological observation of cecal tonsil and changes of the cecal tonsil T cell subsets by method of flow cytometry. Four hundred twenty 1-day-old avian broilers were divided into six groups and fed on a corn-soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, and 60 mg/kg vanadium supplied as ammonium metavanadate. In comparison with those of control group, lymphocytes in the lymphatic nodule of cecal tonsil were apparently decreased in 45 and 60 mg/kg groups. The percentage of CD(3)(+) T cells was decreased (p?0.05) in 45 mg/kg group at 28 and 42 days of age and significantly decreased (p?0.01) in 60 mg/kg group at 28 and 42 days of age. The percentages of CD(3)(+)CD(4)(+) and CD(3)(+)CD(8)(+) T cells were markedly decreased (p?0.05 or p?0.01) in 60 mg/kg group from 14 to 28 days of age and were decreased (p?0.05) in 45 mg/kg group at 28 and 42 days of age. However, changes of the CD(4)(+)/CD(8)(+) ratio were not significant. Meanwhile, the cecal tonsil interleukin-2 (IL-2) contents were decreased (p?0.05 or p?0.01) in 45 and 60 mg/kg groups from 14 to 42 days of age. It was concluded that dietary vanadium in excess of 30 mg/kg reduced the percentages of cecal tonsil T cells subsets and IL-2 contents, and caused cecal tonsil lesions, which impaired cecal tonsil function and impacted the local mucosal immune function of the intestines in broilers. 相似文献
3.
The purpose of this 42-day study was to investigate the apoptosis in the bursa of Fabricius induced by different levels of
dietary vanadium. A total of 420 1-day-old avian broilers were divided into 6 groups in which there were 7 replicates in each
group and 10 broilers in each replicate and fed on a corn–soybean basal diet as control diet (vanadium 0.073 mg/kg) or the
same diet amended to contain 5, 15, 30, 45, and 60 mg/kg vanadium supplied as ammonium metavanadate (NH4VO3). Ultrastructurally, mitochondrial injury and increased numbers of apoptotic cells with condensed nuclei were observed in
the 30, 45, and 60 mg/kg groups. As measured by flow cytometry, the percentages of apoptotic lymphocytes were significantly
increased in the 15-, 30-, 45-, and 60-mg/kg groups when compared with those of control group. Meanwhile, the terminal deoxynucleotidyl
transferase 2′-deoxyuridine 5′-triphosphate nick end-labeling assay showed that there were increased numbers of apoptotic
cells in the 30-, 45-, and 60-mg/kg groups. Immunohistochemical tests showed increased numbers of positive cells under Bax
and caspase-3 protein detection and decreased Bcl-2 protein in the 15-, 30-, 45-, and 60-mg/kg groups. The vanadium content
of the bursa was found to be significantly increased in the 30-, 45-, and 60-mg/kg groups. These results suggested that dietary
vanadium in excess of 15 mg/kg could cause lymphocyte apoptosis in the bursa of Fabricius and impact humoral immunity in broilers.
Lymphocyte apoptosis in the bursa induced by high levels of dietary vanadium is associated with mitochondrial injury and changes
in levels of apoptogenic proteins, such as Bcl-2, Bax, and caspase-3. 相似文献
4.
Liu J Cui H Liu X Peng X Deng J Zuo Z Cui W Deng Y Wang K 《Biological trace element research》2012,145(2):189-200
The purpose of this study was to investigate the renal and hepatic oxidative damage and toxicity caused by dietary high vanadium
in broilers. A total of 420 one-day-old avian broilers were divided into six groups and fed on a corn–soybean basal diet as
control diet (vanadium 0.073 mg/kg), and five high vanadium diets (vanadium 5 mg/kg, high vanadium group I; 15 mg/kg, high
vanadium group II; 30 mg/kg, high vanadium group III; 45 mg/kg, high vanadium group IV; and 60 mg/kg, high vanadium group
V) throughout the experimental period of 42 days. The results showed that the renal and hepatic superoxide dismutase (SOD)
and glutathione peroxidase (GSH-Px) activities, ability to inhibit hydroxy radical, and malondialdehyde (MDA), glutathione,
and vanadium contents were not significantly changed in high vanadium group I and II when compared with those of the control
groups. However, the SOD and GSH-Px activities, ability to inhibit hydroxy radical, and GSH content were significantly decreased,
and the MDA and vanadium contents were markedly increased in high vanadium groups III, IV, and V. At the same time, the lesions
were also observed in the kidney and liver of high vanadium groups III, IV, and V. The renal tubular epithelial cells showed
granular degeneration and vacuolar degeneration, and hepatocytes showed granular degeneration, vacuolar degeneration, and
fatty degeneration. It was concluded that dietary vanadium in the range of 30–60 mg/kg could cause oxidative damage and vanadium
accumulation, which induced renal and hepatic toxicity and lesions. The renal and hepatic function was finally impaired in
boilers. 相似文献
5.
Wang K Cui H Deng Y Peng X Fang J Zuo Z Cui W 《Biological trace element research》2012,147(1-3):113-119
The purpose of this 42-day study was to examine the effect of dietary vanadium on the ileac T cells and contents of cytokines including interleukin-2 (IL-2), interleukin-6 (IL-6), and interferon-gamma (IFN-γ) in broilers by flow cytometry and enzyme-linked immunosorbent assay. A total of 420 one-day-old avian broilers were divided into six groups (seven replicates in each group and ten broilers in each replicate) and fed on control diet or the same diet supplemented with 5, 15, 30, 45, and 60 mg/kg vanadium in the form of ammonium metavanadate. The results showed that the percentages of CD3(+), CD3(+)CD4(+), and CD3(+)CD8(+) T cells in both ileac lamina propria lymphocytes (LPLs) and intraepithelial lymphocytes (IELs) were significantly lower (P < 0.05 or P < 0.01) in the 45- and 60-mg/kg groups than in the control group from 14 to 42 days of age. The CD4(+)/CD8(+) ratio was increased in ileac LPLs in the 60-mg/kg group at 28 days of age, and in ileac IELs in the 60-mg/kg group at 28 days of age and in the 45-mg/kg group at 42 days of age. Meanwhile, the ileac IL-2, IL-6 contents were decreased (P < 0.05 or P < 0.01) in the 60-mg/kg group from 14 to 42 days of age and in the 45-mg/kg group from 28 to 42 days of age in comparison with those of the control group. It was concluded that dietary vanadium in excess of 30 mg/kg reduced the ileac T cell population and percentages of T cell subsets, and IL-2, IL-6, and IFN-γ contents, implying that the immune function of local intestinal mucosa in broilers could be affected by the dietary vanadium. 相似文献
6.
Deng Y Cui H Peng X Fang J Zuo Z Wang K Cui W Wu B 《Biological trace element research》2012,147(1-3):149-155
The cecal tonsil of broiler is known as a secondary lymphoid tissue, which is involved in antigen-specific humoral immune responses. The purpose of this study was to investigate the effects of dietary vanadium on the tissue distribution and quantity of immunoglobulin A-positive (IgA(+)) cell in the cecal tonsil by immunohistochemistry. Simultaneously, the changes in interleukin-6 (IL-6), interleukin-10 (IL-10), interferon gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) contents in the cecal tonsil were also quantified by enzyme-linked immunosorbent assay (ELISA). A total of 420 one-day-old avian broilers were divided into six groups and fed on a corn-soybean basal diet (control diet) or the same diet supplemented respectively with 5, 15, 30, 45, and 60 mg/kg of vanadium in the form of ammonium metavanadate for 42 days. The results showed that the population of the IgA(+) cells in the cecal tonsil were significantly lower (p < 0.05 or p < 0.01) in the 45 and 60 mg/kg groups than that in the control group. Meanwhile, IL-10, IFN-γ and TNF-α contents in the cecal tonsil were significantly decreased (p < 0.05 or p < 0.01) in the 30, 45 and 60 mg/kg groups in comparison with those of the control group. However, IL-6 content in the cecal tonsil was only decreased (p < 0.05 or p < 0.01) in 60 mg/kg at 14 and 28 days of age. In conclusion, dietary vanadium in excess of 30 mg/kg reduced the numbers of the IgA(+) cells and changed the contents of the abovementioned cytokines in the cecal tonsil, which may finally impact the function of local mucosal humoral immunity in broilers. 相似文献
7.
The purpose of this study was to examine oxidative stress induced by dietary vanadium in the mucosa of different parts of
intestine including duodenum, jejunum, ileum, and cecal tonsil. A total of 420 1-day-old avian broilers were divided into
six groups and fed on a corn–soybean basal diet as control diet or the same basal diet supplemented with 5, 15, 30, 45, and
60 mg/kg vanadium as ammonium metavanadate. During the experimental period of 42 days, oxidative stress parameters were determined
for both control and experimental groups. The results showed that malondialdehyde content was significantly higher (p < 0.05 or p < 0.01) in 30, 45, and 60 mg/kg groups than in control group. In contrast, the activities of superoxide dismutase, catalase,
and glutathione peroxidase, and ability to inhibit hydroxyl radical, and glutathione hormone content were significantly decreased
(p < 0.05 or p < 0.01) mainly in 45 and 60 mg/kg groups in comparison with those of control group. However, the abovementioned oxidative
stress parameters were not significantly changed (p > 0.05) in 5 and 15 mg/kg groups. It was concluded that dietary vanadium in excess of 30 mg/kg could cause obvious oxidative
stress in the intestinal mucosa, which could impact the antioxidant function of intestinal tract in broilers. 相似文献
8.
The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on spleen growth and lesions by
determining morphological changes and cell cycle of spleen. Four hundred twenty 1-day-old avian broilers were divided into
six groups and fed on a corn–soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, 60 ppm
of vanadium supplied as ammonium metavanadate. When compared with that of control group, the relative weight of spleen was
significantly raised in 5- and 15-ppm groups, but depressed in 45- and 60-ppm groups. The gross lesions of spleen showed obvious
atrophy with decreased volume and pale color in 45- and 60-ppm groups. Histopathologically, lymphocytes in splenic corpuscle
and periarterial lymphatic sheath were variously decreased in number in 30-, 45-, and 60-ppm groups. The percentage of static
phase (G0/G1) was significantly decreased, and the percentage of synthesis period (S) phase and the proliferating index (PI) were significantly
increased in 5- and 15-ppm groups. The percentage of G0/G1 phase was significantly increased, and the percentage of mitotic phase (G2 + M), S phase, and PI significantly decreased in 45- and 60-ppm groups. These results suggested that dietary excess vanadium
(45 and 60 ppm) could inhibit growth of spleen and induce lesions in spleen in chicken. 相似文献
9.
Peng X Cui H Yuan J Cui W Fang J Zuo Z Deng J Pan K Zhou Y Lai W 《Biological trace element research》2011,144(1-3):688-694
The purpose of this 42-day study was to investigate the effects of low selenium (Se) on cellular immune function by determining cell cycle of thymus, serum IL-2 content, and mitogenesis of peripheral blood T-lymphocytes. One hundred twenty 1-day-old Avian broilers were randomly assigned to two groups of 60 each and were fed on a low-Se diet (0.0342?mg/kg Se) or a control diet (0.2?mg/kg Se), respectively. Cell cycle analysis by flow cytometry showed that low-Se diet caused an increase in G(0)G(1) phase cells that corresponded to a decrease in S-phase cells in thymus. Ultrastructurally, mitochondria injury and increased apoptotic cells with condensed nuclei were observed. Low-Se diet decreased the serum IL-2 contents and mitogenesis of peripheral blood lymphocytes to concanavalin A in comparison with those of control group. These data indicate that low-Se diet inhibits the development of thymus by arresting the cell cycle and decreasing the IL-2 content. 相似文献
10.
Peng X Cui H Fang J Zuo Z Deng J Pan K Lai W Zhou Y 《Biological trace element research》2012,148(2):182-186
The purpose of this 42-day study was to investigate the effects of low selenium (Se) on histopathological changes of spleen, cell cycle and apoptosis of splenocytes, and oxidative status of the spleen. One hundred twenty 1-day-old avian broilers were randomly divided into two groups of 60 each and were fed on a low Se diet (0.0342 mg/kg Se) or a control diet (0.2 mg/kg Se), respectively. The weight and relative weight of the spleen were significantly decreased in the low Se group when compared with those of the control group. Histopathologically, splenic lesions in low-Se chicken were characterized by lymphocyte depletion and congestion of red pulp. As measured by flow cytometry, splenocytes in G(0)/G(1) phase were significantly increased, while splenocytes in S phase and G(2) + M phase were obviously decreased in the low Se group. The percentage of apoptotic splenocytes was greatly increased in the low Se group when compared with that of control group. At the same time, the occurrence frequencies of apoptotic splenocytes was markedly increased in the low Se group with the appearance of condensed nucleus ultrastructurally. Oxidative stress in the spleens of the low Se group was evidenced by decrease in glutathione peroxidase and catalase activities and increase in malondialdehyde contents. The results showed that low Se diet intake caused increased apoptosis, arrested cell cycle, and obvious oxidative stress, which provided a possible pathway for the injured structure and immune function of the spleen in chickens. 相似文献
11.
Wei Cui Hengmin Cui Xi Peng Jing Fang Zhicai Zuo Xiaodong Liu Bangyuan Wu 《Biological trace element research》2011,143(1):251-260
The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on immune function by determining
the morphological changes and cell cycle of bursa of Fabricius, and the serum Ig contents. A total of 420 one-day-old avian
broilers were divided into six groups and fed on a corn–soybean basal diet as control diet, or the same diet amended to contain
5, 15, 30, 45, and 60 ppm vanadium supplied as ammonium metavanadate. When compared with that of control group, the relative
weight of bursa was significantly increased in the 15 ppm group from 14 to 35 days of age and increased in the 5 ppm group
at 42 days of age, and significantly decreased in the 60 ppm group from 14 to 42 days of age and decreased in 30 and 45 ppm
groups from 35 to 42 days of age. Pathological lesions progressed as the dietary vanadium increased. The gross lesions of
bursa showed obvious atrophy with decreased volume and pale color in 45 and 60 ppm groups. Histopathologically, widened cortex
and increased number of lymphocytes appeared in 5 and 15 ppm groups, and reduced lymphocytes and connective tissue hyperplasia
appeared in 45 and 60 ppm groups. The bursal cells in static phase (G0/G1) were decreased, and those in the mitotic phase (G2 + M) and the proliferating index (PI) were increased in 5 and 15 ppm groups. However, bursal cells in the G0/G1 phase were increased, and those in G2 + M phase, synthesis phase (S) and the PI were decreased in 45 and 60 ppm groups. Also, the serum IgG and IgA contents were
increased in 5 and 15 ppm groups, and the serum IgG, IgA, and IgM contents were decreased in 45 and 60 ppm groups. These results
suggested that dietary excess vanadium (45 and 60 ppm) could inhibit growth of bursa of Fabricius and impair humoral immunity
in chicken. 相似文献
12.
Xi Peng Hengmin Cui Junliang Deng Zhicai Zuo Weiming Lai 《Biological trace element research》2011,140(1):66-72
The purpose of this 42-day study was to investigate the effects of excess dietary selenium on immune function by determining
morphological changes of spleen and cell cycle of splenocyte. Three hundred 1-day-old avian broilers were fed on a basic diet
(0.2 mg/kg selenium) or the same diet amended to contain 1, 5, 10, and 15 mg/kg selenium (Se) supplied as sodium selenite
(n = 60/group). Anatomically, the spleens were shrinked in volume with pallecent color. Histopathologically, lymphopenia in
splenic nodules and periarterial lymphatic sheaths and congestion of the red pulp were observed in 5, 10, and 15 mg/kg Se
group. By flow cytometry method, the percentage of G0/G1 phase splenocytes was significantly increased, whereas the percentages of S phase and G2+M phase splenocytes and the proliferation index were markedly decreased in 5, 10, and 15 mg/kg Se groups when compared with
those of 0.2 mg/kg group. The results confirmed that excess dietary Se as sodium selenite in the range of 5∼15 mg/kg caused
growth retardation of spleen by cell cycle blockage in young chickens. 相似文献
13.
The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on immune function by determining
changes of the subsets and proliferation function of splenic T cells. Four hundred twenty 1-day-old avian broilers were divided
into six groups and fed on a corn–soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, and
60 ppm of vanadium supplied as ammonium metavanadate. When compared with those of the control group, the percentage of CD3+, CD3+CD4+, and CD3+CD8+ of splenic T cells were decreased in the 45 and 60 ppm groups; however, the percentage of CD3+ and CD3+CD4+ were increased in the 5 ppm group, and the CD4+/CD8+ ratios were raised in the 5 and 15 ppm groups at 14 days of age. Meanwhile, the proliferation of splenic T cells were depressed
in the 45 and 60 ppm groups but raised in the 5 and 15 ppm groups. Also, the serum interleukin-2 (IL-2) and interleukin-6
(IL-6) contents were decreased in the 45 and 60 ppm groups and increased in the 5 ppm group. It was concluded that dietary
vanadium in excess of 30 ppm changed the percentages of splenic T cell subsets and inhibited the proliferation of splenic
T cells and reduced the serum IL-2 and IL-6 contents. The cellular immune function was finally impaired in broilers. 相似文献
14.
Jie Xiao Heng-min Cui Fan Yang Xi Peng Yun Cui 《Biological trace element research》2011,142(3):523-531
The experiment was conducted with the objective of examining the effects of high molybdenum on the cell cycle and apoptosis of kidney in broilers by the methods of flow cytometry. Three hundred 1-day-old Avian broilers were randomly divided into four groups, and fed on diets as follows: control diet (Mo 13 mg/kg) and high molybdenum diets (Mo 500 mg/kg, high molybdenum group I; Mo 1,000 mg/kg, high molybdenum group II; Mo 1,500 mg/kg, high molybdenum group III) for 6 weeks. The results showed that the relative weight of kidney were higher (P?<?0.05 or P?<?0.01), and the cellular percentages of G0/G1 phase were lower, and cellular percentages of S phase and the proliferating index were higher in high molybdenum groups II and III than in control group (P?<?0.01). The percentage of renal cell apoptosis was increased in high molybdenum groups II and III when compared with that of control group (P?<?0.01). Immunohistochemical test showed that there were increased frequencies of positive cells containing Bax protein and decreased frequencies of positive cells containing Bcl-2 protein in high molybdenum groups II and III. It was concluded that dietary high molybdenum (1,000 mg/kg and 1,500 mg/kg) impaired the progression of renal cells from S phase to G2M phase obviously and induced renal cell apoptosis. 相似文献
15.
Caimin Bai Tao Chen Yun Cui Tao Gong Xi Peng Heng-min Cui 《Biological trace element research》2010,138(1-3):173-180
The experiment was conducted with the objective of evaluating the effect of dietary high fluorine (F) on cell cycle and apoptosis of kidney in chickens by the methods of flow cytometry. Three hundred 1-day-old Avian broilers were divided into four groups and fed on control diet (F 23 mg/kg) and high F diets (400 mg/kg, high F group I; 800 mg/kg, high F group II; 1,200 mg/kg, high F group III) for 6 weeks. As tested by flow cytometry, the percentage of renal cell apoptosis was increased with increasing of dietary F, and it obviously rose in three high F groups when compared with that of control group. Renal cells in G0/G1 phase were much higher, and renal cells in S phase, G2+M phase, and proliferation index value were much lower in high F groups I, II, and III than in control group. The results showed that excess dietary F in the range of 400–1,200 mg/kg caused G0/G1 arrest and increased cellular apoptosis in the kidney, which might finally interfere with the excretion and retention of fluoride in chickens. 相似文献
16.
Xi Peng Yun Cui Wei Cui Junliang Deng Hengmin Cui Fan Yang 《Biological trace element research》2011,139(1):32-40
The purpose of this 42-day study was to investigate the effects of low selenium (Se) on immune function by determining cell
cycle and apoptosis of bursa of Fabricius. One hundred twenty 1-day-old avian broilers were randomly assigned to two groups
of 60 each and were fed on a low Se diet (0.0342 mg/kg Se) or a control diet (0.2 mg/kg Se), respectively. The relative weight
of bursa was significantly decreased in low Se group from 28 days of age in time-dependent manner when compared with that
of control group. Cell cycle analysis by flow cytometry showed that low Se caused an increase in G0G1 phase cells that corresponded to a decrease in S phase cells in bursa of Fabricius. Ultrastructurally, mitochondria injury
and increased apoptotic cells with condensed nuclei were observed. Low Se increased the percentage of Annexin V-positive cells,
as measured by flow cytometry, in comparison with that of control group. These data suggested that low Se diet restrained
the development of bursa of Fabricius by cell cycle arrest and apoptosis. 相似文献
17.
Peng X Cui H He Y Cui W Fang J Zuo Z Deng J Pan K Zhou Y Lai W 《Biological trace element research》2012,145(1):47-51
Three hundred 1-day-old avian broilers were fed on a basic diet (0.2 mg/kg selenium) or the same diet amended to contain 1,
5, 10, and 15 mg/kg selenium supplied as sodium selenite (n = 60/group). In comparison with those of 0.2 mg/kg selenium group, the percentages of annexin V-positive splenocytes were
increased in 5, 10, and 15 mg/kg selenium groups. TUNEL assay revealed that apoptotic cells with brown-stained nuclei distributed
within the red pulp and white pulp of the spleens with increased frequency of occurrence in 10 and 15 mg/kg selenium groups
in comparison with that of 0.2 mg/kg Se group. Sodium selenite-induced oxidative stress in spleens of chickens was evidenced
by decrease in glutathione peroxidase, superoxide dismutase, and catalase activities and increase in malondialdehyde contents.
The results indicate that excess dietary selenium in the range of 5–15 mg/kg of feed causes oxidative stress, which may be
mainly responsible for the increased apoptosis of splenocytes in chickens. 相似文献
18.
Fan Yang Hengmin Cui Jie Xiao Xi Peng Junliang Deng Zhicai Zuo 《Biological trace element research》2011,139(3):308-316
The experiment was conducted with the objective of examining the effects of high molybdenum (Mo) on the apoptosis of splenic
lymphocytes in broilers by the methods of experimental pathology and flow cytometry (FCM). Three hundred 1-day-old avian broilers
were divided into four groups of 75 each and were fed on control diet (Mo 13 mg/kg) and high Mo diets (Mo 500 mg/kg, high
Mo group I; Mo 1,000 mg/kg, high Mo group II; and Mo 1,500 mg/kg, high Mo group III) for 42 days. The results showed that
the relative weight of spleen was decreased, and lymphocytes were histopathologically decreased in high Mo groups II and III.
Ultrastructurally, the apoptotic cells showed typical condensed nuclei with dark-round, petal-like, and horseshoe-like shapes.
The mitochondria were swelled, and the endoplasmic reticulum and the Golgi apparatus were dilated in high Mo groups II and
III. The statistical analyses by FCM indicated that the percentage of cellular apoptosis was higher in high Mo groups II and
III than in control group. Also, the TUNEL staining was consistent with the results of FCM. It was concluded that dietary
molybdenum in 1,000 and 1,500 mg/kg caused splenic lesions and lymphocyte apoptosis, which could inhibit the development of
spleen and could impair immune function in broilers. 相似文献
19.
The purpose of this study was to determine the effect of polyphenols extracted from the tamarind seed coat (PETSC) on glutathione peroxidase (GPx) activity, red blood cell parameters and bilirubin in heat-stressed broilers. One hundred forty-seven broilers, 18-days old were divided into two groups. In group 1, broilers were maintained at an environmental temperature of 26?±?2 °C throughout the experimental period. In group 2, the broilers were maintained at 38?±?2 °C (cyclic temperature: 26?±?2 °C; ?38?±?2 °C; and ?26?±?2 °C, and broilers were maintained at 38?±?2 °C for 6 h/ day) and received PETSC at a concentration of 0, 100, 200, 300, 400 or 500 mg/kg in their diet ad libitum. Parameters were investigated on days 1, 7, 14 and 21 of the experimental period. Results showed that GPx activity of heat-stressed broilers that received 100 mg/kg of PETSC in their diet was lower (P?<?0.05) than that in broilers fed the other concentrations. The mean total red blood cell count and hemoglobin concentration of heat-stressed broilers that received 100 mg/kg PETSC was higher (P?<?0.05) than those in broilers in group 1 and those fed the other concentrations. The mean bilirubin level in the excreta of heat-stressed broilers that received 100 mg/kg of PETSC was lower (P?<?0.05) than that in broilers that received 0, 300, 400 and 500 mg/kg of PETSC. This showed that PETSC could reduce GPx activity and bilirubin in feces, and increase red blood cell parameters in heat-stressed broilers. 相似文献
20.
Wei Cui Heng-min Cui Xi Peng Zhicai Zuo Xiaodong Liu Bangyuan Wu 《Biological trace element research》2011,141(1-3):192-199
The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on immune function by determining changes of the subsets and proliferation function of peripheral blood T cells. Four hundred twenty 1-day-old avian broilers were divided into six groups and fed on a corn–soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, and 60 ppm vanadium supplied as ammonium metavanadate. In comparison with those of the control group, the percentages of CD 3 + , CD 3 + CD 4 + , and CD 3 + CD 8 + were decreased in 45 and 60 ppm groups from 14 to 42 days of age, and the percentages of CD 3 + and CD 3 + CD 4 + were increased in 5 ppm group at 42 days of age. The CD 4 + /CD 8 + ratio was increased in 45 and 60 ppm groups at 28 days of age. Meanwhile, the proliferation function of peripheral blood T cell were decreased in 30, 45, and 60 ppm groups from 14 to 42 days of age. Also, the serum interleukin-2 contents were decreased in 45 and 60 ppm groups from 14 to 42 days of age and increased in 5 ppm group at 28 days of age. Histopathologically, hypocellularity appeared in the thymus in 45 and 60 ppm groups. It was concluded that dietary vanadium in excess of 30 ppm reduced the percentages of peripheral blood T-cell subsets and the proliferation function and serum interleukin-2 contents. The cellular immune function was finally impaired in broilers. 相似文献