共查询到20条相似文献,搜索用时 31 毫秒
1.
The linkage map of sheep Chromosome 6 compared with orthologous regions in other species 总被引:9,自引:0,他引:9
E. A. Lord J. M. Lumsden K. G. Dodds H. M. Henry A. M. Crawford H. A. Ansari P. D. Pearce D. W. Maher R. T. Stone S. M. Kappes C. W. Beattie G. W. Montgomery 《Mammalian genome》1996,7(5):373-376
The genetic linkage map of sheep Chromosome (Chr) 6 has been extended to include 35 loci with the addition of 11 RFLP and
12 microsatellite loci. The sex-averaged linkage map now spans 154 cM from phosphodiesterase cyclic GMP beta polypeptide (PDE6B) to OarCP125, an anonymous sheep microsatellite. The male and female map lengths, at 180 cM and 132 cM respectively, did not differ significantly.
The physical assignment of PDE6B to Chr 6q33-qter orientates the linkage map on sheep Chr 6 with PDE6B near the telomere and OarCP125 towards the centromere. The order and genetic distances between loci are similar for the sheep Chr 6 and cattle Chr 6 maps,
except for the position of the casein genes. The sheep Chr 6 linkage map is also comparable to portions of human Chr 4, mouse
Chrs 5 and 3, and pig Chr 8. The synteny between sheep Chr 6 and human Chr 4 has been extended from PDE6B (4p16.3) to epidermal growth factor (EGF, 4q25-q27). However, a region from platelet-derived growth factor receptor α polypeptide (PDGFRA) to bone morphogenetic protein 3 (BMP3), which spans 19 cM on sheep Chr 6, appears to be inverted with respect to the human and mouse loci. Other differences in
the gene order between sheep, pig, and mouse suggest more complex rearrangements.
Received: 16 August 1995 / Accepted: 12 December 1995 相似文献
2.
Päivi Pajukanta Jackie S. Bodnar Riitta Sallinen Michael Chu Tuula Airaksinen Qunong Xiao Lawrence W. Castellani Sonal S. Sheth Maija Wessman Aarno Palotie Janet S. Sinsheimer Peter Demant Aldons J. Lusis Leena Peltonen 《Mammalian genome》2001,12(3):238-245
Familial combined hyperlipidemia (FCHL) is a common genetic dyslipidemia predisposing to premature coronary heart disease
(CHD). We previously identified a locus for FCHL on human Chromosome (Chr) 1q21-q23 in 31 Finnish FCHL families. We also mapped
a gene for combined hyperlipidemia (Hyplip1) to a potentially orthologous region of mouse Chr 3 in the HcB-19/Dem mouse model of FCHL. The human FCHL locus was, however, originally mapped about 5 Mb telomeric to the synteny border, the
centromeric part of which is homologous to mouse Chr 3 and the telomeric part to mouse Chr 1. To further localize the human
Hyplip1 homolog and estimate its distance from the peak linkage markers, we fine-mapped the Hyplip1 locus and defined the borders of the region of conserved synteny between human and mouse. This involved establishing a physical
map of a bacterial artificial chromosome (BAC) contig across the Hyplip1 locus and hybridizing a set of BACs to both human and mouse chromosomes by fluorescence in situ hybridization (FISH). We
narrowed the location of the mouse Hyplip1 gene to a 1.5-cM region that is homologous only with human 1q21 and within approximately 5–10 Mb of the peak marker for linkage
to FCHL. FCHL is a complex disorder and this distance may, thus, reflect the well-known problems hampering the mapping of
complex disorders. Further studies identifying and sequencing the Hyplip1 gene will show whether the same gene predisposes to hyperlipidemia in human and mouse.
Received: 9 September 2000 / Accepted: 30 October 2000 相似文献
3.
4.
By means of somatic cell hybrids segregating rat chromosomes, we determined the chromosome localization of three rat genes of the Jun family: Jumb (Chr 19), Jun (=c-Jun) (Chr 5) and Jund (Chr 16). The Jun gene was also localized to the 5q31–33 region by fluorescence in situ hybridization. These rat gene assignments reveal two new homologies with mouse and human chromosomes, and provide a new example of synteny conserved in the human and a rodent species (the mouse), but split between the two rodent species. 相似文献
5.
The porcine genes encoding the immunoglobulin gamma heavy chain (IGHG), cAMP-dependent protein kinase catalytic beta subunit (PRKACB), and transition protein 2 (TNP2) were mapped to Chromosomes (Chrs) 7 q25–q26, 6q31–q33, and 3p13-cent, respectively, by in situ hybridization. Localization of the IGHG gene confirms the assignment of linkage group III to Chr 7. Our results show that the IGHG locus in pigs, similar to the situation in other mammalian species, viz. humans, mouse, cattle, and river buffaloes, is located on the terminal region of the chromosome. The assignment of the PRKACB gene extends the homology observed between porcine Chr 6q and human Chr 1p. Mapping of the TNP2 gene provides the first marker assigned to the p arm of Chr 3 in pigs. The present study contributes to the development of the physical gene map in pigs and also bears significance in terms of comparative gene mapping. 相似文献
6.
Multiple obesity QTLs identified in an intercross between the NZO (New Zealand obese) and the SM (small) mouse strains 总被引:2,自引:0,他引:2
Benjamin A. Taylor Christopher Wnek David Schroeder Sandra J. Phillips 《Mammalian genome》2001,12(2):95-103
The inheritance of adiposity levels has been investigated in an intercross of the obese, diabetes-prone NZO and the small,
lean SM mouse strains. Adiposity index (AI) was defined as the sum of four fat pad weights divided by body weight. DNA pools
from fat and lean mice were analyzed with microsatellite variants to screen the genome for quantitative trait loci (QTLs)
affecting AI. Ten significant QTLs affecting AI were identified on Chromosome (Chr) 1 (three loci), Chr 2, Chr 5 (two loci),
Chr 6 (two loci), Chr 7, and Chr 17. Most of the QTLs appear to be novel. Several QTLs differentially affect specific fat
depots. Thus, Chr 2 and Chr 7 QTLs affect gonadal more than inguinal fat, while the converse is true for the Chr 17 QTL. Gender
influences the expression of several of the QTLs. For example, effects of the proximal Chr 1 QTL (Obq7) on AI appears to be primarily in males. The proximal AI QTL on Chr 6 (Obq13) maps near the neuropeptide Y (Npy) locus. Sequence analysis of the Npy gene revealed a 1-nucleotide deletion within a highly conserved portion of the 3′ untranslated region in strain NZO. However,
the deletion is polymorphic among mouse strains. Furthermore, lack of association between this same variant and AI in previously
analyzed crosses raises doubt that it is the basis of Obq13. The present cross is the fourth in a series of intercrosses among 10 inbred strains arranged such that each strain is crossed
with each adjacent strain within a circle. This design affords multiple opportunities to analyze each segregating QTL.
Received: 17 July 2000 / Accepted: 9 October 2000 相似文献
7.
8.
A radiation hybrid map of the RN region in pigs demonstrates conserved gene order compared with the human and mouse genomes 总被引:2,自引:0,他引:2
Annie Robic Virginie Seroude Jin-Tae Jeon Martine Yerle Luc Wasungu Leif Andersson Joël Gellin Denis Milan 《Mammalian genome》1999,10(6):565-568
We recently constructed a 7000-rad porcine whole-genome radiation hybrid (RH) panel with the primary objective of integrating
linkage maps of microsatellites with evolutionary conserved genes into one ordered map. In order to evaluate the resolution
of this RH panel, we have now constructed a radiation hybrid map of the Chromosome (Chr) 15q2.3-q2.6 region containing the
RN gene. This gene has large effects on glycogen content in muscle and meat quality. Ten microsatellites covering a region of
55 centiMorgans and eight genes (AE3, FN1, IGFBP5, INHA, IRS1, PAX3, TNP1, and VIL1) were placed on the Sscr15 RH map. All the genes, except IRS1, were mapped on the RH map between microsatellites located in 15q2.5. The relative order of AE3 and INHA was inverted on the porcine physical map in comparison with the mouse linkage map. The order of other genes already mapped
in the mouse (FN1, IGFBP5, TNP1, VIL1, INHA/AE3, and PAX3) was identical in pigs. We found no clear difference between the gene order on pig Chr 15 and human Chr 2q.
Received: 4 November 1998 / Accepted: 8 February 1999 相似文献
9.
Hall Richard J. Hollis-Moffatt Jade E. Merriman Marilyn E. Green Rachel A. Baker David Merriman Tony R. 《Mammalian genome》2003,14(5):335-339
Twenty-four named Idd loci that contribute to the development of autoimmune diabetes in the nonobese diabetic (NOD) mouse have been mapped by linkage and congenic analysis. Previously, meta-analysis of genome-wide linkage scans supported the existence of a locus for susceptibility to autoimmune phenotypes on rodent Chromosome (Chr) 18, in a position orthologous to the human type 1 diabetes susceptibility locus IDDM6 (human Chr 18q12-q23). However, an autoimmune diabetes susceptibility locus has not previously been reported on mouse Chr 18. In this study, we demonstrate linkage of the majority of mouse Chr 18 to diabetes in a (ABH × NOD)F1 × NOD backcross. Congenic analysis, introgressing at least 92% of Biozzi ABH Chr 18 onto the NOD background, confirmed the presence of a diabetes locus. The chromosome substitution strain (NOD.ABH-Chr18) had reduced diabetes incidence compared with NOD mice (P < 0.0001). We have named the Chr 18 diabetes locus Idd21. 相似文献
10.
A genome-wide scan for quantitative trait loci (QTLs) controlling body weight at 10 weeks after birth was carried out in
a population of 387 intersubspecific backcross mice derived from a cross between C57BL/6J inbred mice (Mus musculus domesticus) and wild mice (M. m. castaneus) captured in the Philippines, in order to discover novel QTLs from the wild mice that have about 60% lower body weight than
C57BL/6J. By interval mapping, we detected four QTLs: a highly significant QTL on Chromosome (Chr) 2, which was common in
both sexes; two significant QTLs on Chr 13, one male-specific and the other female-specific; and a suggestive male-specific
QTL on X Chr. By composite interval mapping, we confirmed the presence of the three QTLs on Chrs 2 and 13, but not of the
male-specific X-linked QTL. The composite interval mapping analysis newly identified three QTLs: a significant male-specific
QTL on Chr 11 and two highly significant female-specific QTLs on Chrs 9 and X. Individual QTLs explained 3.8–11.6% of the
phenotypic variance, and all the QTL alleles derived from the wild mice decreased body weight. A two-way analysis of variance
revealed a significant epistatic interaction between the Chr 2 QTL and the background marker locus D12Mit4 on Chr 12 only in males. The interaction effect unexpectedly increased body weight. The chromosomal region containing the
Chr 2 QTL did not coincide with those of growth or fatness QTLs mapped in previous studies. These results suggest that a population
of wild mice may play an important role as new sources of valuable QTLs.
Received: 14 January 2000 / Accepted: 14 April 2000 相似文献
11.
Genomic structure, mapping, and expression analysis of the mammalian Lunatic, Manic, and Radical fringe genes 总被引:3,自引:0,他引:3
Jennifer L. Moran Stuart H. Johnston Cordelia Rauskolb Jayant Bhalerao Anne M. Bowcock Thomas F. Vogt 《Mammalian genome》1999,10(6):535-541
The three members of the mammalian fringe gene family, Manic fringe (Mfng), Radical fringe (Rfng), and Lunatic fringe (Lfng), were identified on the basis of their similarity to Drosophila fringe (fng) and their participation in the evolutionarily conserved Notch receptor signaling pathway. Fringe genes encode pioneer secretory
proteins with weak similarity to glycosyltransferases. Both expression patterns and functional studies support an important
role for Fringe genes in patterning during embryonic development and an association with cellular transformation. We have
now further characterized the expression and determined the chromosomal localization and genomic structure of the mouse Mfng, Rfng, and Lfng genes; the genomic structure and conceptual open reading frame of the human RFNG gene; and the refined chromosomal localization
of the three human fringe genes. The mouse Fringe genes are expressed in the embryo and in adult tissues. The mouse and human
Fringe family members map to three different chromosomes in regions of conserved synteny: Mfng maps to mouse Chr 15, and MFNG maps to human Chr 22q13.1 in the region of two cancer-associated loci; Lfng maps to mouse Chr 5, and LFNG maps to human Chr 7p22; Rfng maps to mouse Chr 11, and RFNG maps to human Chr 17q25 in the minimal region for a familial psoriasis susceptibility locus.
Characterization of the genomic loci of the Fringe gene family members reveals a conserved genomic organization of 8 exons.
Comparative analysis of mammalian Fringe genomic organization suggests that the first exon is evolutionarily labile and that
the Fringe genes have a genomic structure distinct from those of previously characterized glycosyltransferases.
Received: 19 February 1999 / Accepted: 22 February 1999 相似文献
12.
We have mapped the gene encoding the murine RYK growth factor receptor protein tyrosine kinase by genetic linkage analysis with recombinant inbred strains of mouse. Two distinct Ryk loci (Ryk-1 and Ryk-2) were identified. Ryk-1 mapped to Chromosome (Chr) 9, whereas Ryk-2 mapped to Chr 12. A similar arrangement of RYK-related loci was previously determined in the human. Synteny has already been established between murine Chr 9 in the region of Ryk-1, and human chromosome 3q11–12, the location of the human RYK-1 gene. However, the Ryk-2/RYK-2 loci on murine Chr 12 and human chr 17p13.3 define a new region of synteny. 相似文献
13.
Quantitative trait loci for baseline erythroid traits 总被引:1,自引:0,他引:1
Luanne L. Peters Amy J. Lambert Weidong Zhang Gary A. Churchill Carlo Brugnara Orah S. Platt 《Mammalian genome》2006,17(4):298-309
A substantial genetic contribution underlies variation in baseline peripheral blood counts. We performed quantitative trait
locus/loci (QTL) analyses to identify chromosome (Chr) regions harboring genes influencing the baseline erythroid parameters
in F2 intercrosses between NZW/LacJ, SM/J, and C57BLKS/J inbred mice. We identified multiple significant QTL for red blood cell
(RBC) count, hemoglobin (Hgb) and hematocrit (Hct) levels, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH),
and mean cell hemoglobin concentration (CHCM). We identified four RBC count QTL: Rbcq1 (Chr 1, peak LOD score at 62 cM,), Rbcq2 (Chr 4, 60 cM), Rbcq3 (Chr 11, 34 cM), and Rbcq4 (Chr 10, 60 cM). Three MCV QTL were identified: Mcvq1 (Chr 7, 30 cM), Mvcq2 (Chr 11, 6 cM), and Mcvq3 (Chr 10, 60 cM). Single significant loci for Hgb (Hgbq1, Chr 16, 32 cM), Hct (Hctq1, Chr 3, 42 cM), and MCH (Mchq1, Chr 10, 60 cM) were identified. The data support the existence of a common RBC/MCH/MCV locus on Chr 10. Two QTL for CHCM
(Chcmq1, Chr 2, 48 cM; Chcmq2, Chr 9, 44 cM) and an interaction between Chcmq2 with a locus on Chr 19 were identified. These analyses emphasize the genetic complexity underlying the regulation of erythroid
peripheral blood traits in normal populations and suggest that genes not previously recognized as significantly impacting
normal erythropoiesis exist. 相似文献
14.
Quantitative trait loci for baseline white blood cell count, platelet count, and mean platelet volume 总被引:3,自引:0,他引:3
Luanne L. Peters Weidong Zhang Amy J. Lambert Carlo Brugnara Gary A. Churchill Orah S. Platt 《Mammalian genome》2005,16(10):749-763
A substantial genetic contribution to baseline peripheral blood counts has been established. We performed quantitative trait
locus/loci (QTL) analyses to identify chromosome (Chr) regions harboring genes influencing the baseline white blood cell (WBC)
count, platelet (Plt) count, and mean platelet volume (MPV) in F2 intercrosses between NZW/LacJ, SM/J, and C57BLKS/J inbred mice. We identified six significant WBC QTL: Wbcq1 (peak LOD score at 38 cM, Chr 1), Wbcq2 (42 cM, Chr 3), Wbcq3 (0 cM, Chr 15), Wbcq4 (58 cM, Chr 1), Wbcq5 (82 cM, Chr 1), and Wbcq6 (8 cM, Chr 14). Three significant Plt QTL were identified: Pltq1 (24 cM, Chr 2), Pltq2 (36 cM, Chr 7), and Pltq3 (10 cM, Chr 12). Two significant MPV QTL were identified, Mpvq1 (62 cM, Chr 15) and Mpvq2 (44 cM, Chr 8). In total, the WBC QTL accounted for up to 31% of the total variance in baseline WBC count, while the Plt
and MPV QTL accounted for up to 30% and 49% of the total variance, respectively. These analyses underscore the genetic complexity
underlying these traits in normal populations and provide the basis for future studies to identify novel genes involved in
the regulation of mammalian hematopoiesis. 相似文献
15.
Christine Ambrose Shirley Cheng Bertrand Fontaine Joseph H. Nadeau Marcy MacDonald James F. Gusella 《Mammalian genome》1992,3(3):151-155
Recent evidence suggests that the human neuromuscular disorders, hyperkalemic periodic paralysis and paramyotonia congenita, are both caused by genetic defects in the -subunit of the adult skeletal muscle sodium channel, which maps near the growth hormone cluster (GH) on Chromosome (Chr) 17q. In view of the extensive homology between this human chromosome and mouse Chr 11, we typed an interspecies backcross to determine whether the murine homolog (Scn4a) of this sodium channel gene mapped within the conserved chromosomal segment. The cytosolic thymidine kinase gene, Tk-1, was also positioned on the genetic map of Chr 11. Both Scn4a and Tk-1 showed clear linkage to mouse Chr 11 loci previously typed in this backcross, yielding the map order: Tr
J-(Re, Hox-2, Krt-1)-Scn4a-Tk-1. No mouse mutant that could be considered a model of either hyperkalemic periodic paralysis or paramyotonia congenita has been mapped to the appropriate region of mouse Chr 11. These data incorporate an additional locus into the already considerable degree of homology observed for these human and mouse chromosomes. These data are also consistent with the view that the conserved segment region may extend to the telomere on mouse Chr 11 and on human 17q. 相似文献
16.
A linkage map of mouse Chromosome 1 using an interspecific cross segregating for the gld autoimmunity mutation 总被引:6,自引:0,他引:6
Mark L. Watson Peter D'Eustachio Beverly A. Mock Alfred D. Steinberg Herbert C. Morse III Rebecca J. Oakey Thad A. Howard Julie M. Rochelle Michael F. Seldin 《Mammalian genome》1992,2(3):158-171
An interspecific backross was used to define a high resolution linkage map of mouse Chromosome (Chr) 1 and to analyze the segregation of the generalized lymphoproliferative disease (gld) mutation. Mice homozygous for gld have multiple features of autoimmune disease. Analysis of up to 428 progeny from the backcross [(C3H/HeJ-gld x Mus spretus)F1 x C3H/HeJ-gld] established a map that spans 77.6 cM and includes 56 markers distributed over 34 ordered genetic loci. The gld mutation was mapped to a less than 1 cM segment on distal mouse Chr 1 using 357 gld phenotype-positive backcross mice. A second backcross, between the laboratory strains C57BL/6J and SWR/J, was examined to compare recombination frequency between selected markers on mouse Chr 1. Significant differences in crossover frequency were demonstrated between the interspecific backcross and the inbred laboratory cross for the entire interval studied. Sex difference in meiotic crossover frequency was also significant in the laboratory mouse cross. Two linkage groups known to be conserved between segments of mouse Chr 1 and the long arm of human Chrs 1 and 2 where further defined and a new conserved linkage group was identified that includes markers of distal mouse Chr 1 and human Chr 1, bands q32 to q42. 相似文献
17.
Catherine L. Peichel Stephen W. Scherer Lap-Chee Tsui David R. Beier Thomas F. Vogt 《Mammalian genome》1993,4(11):632-638
Midkine (Mdk) and heparin-binding neurotrophic factor (Hbnf)/pleiotrophin (Ptn) comprise the Midkine family of developmentally regulated signaling molecules. We have determined the chromosomal localization of these genes in the mouse by use of singlestrand conformation polymorphisms (SSCPs), which facilitated the typing of Mdk and Hbnf alleles in recombinant inbred (RI) strains and interspecific backcrosses. Mapping was performed relative to other cloned genes, as well as simple sequence length polymorphisms (SSLPs) in the interspecific backcrosses. Mdk maps to mouse Chromosome (Chr) 2, linked to the Hoxd gene cluster. Hbnf maps to proximal mouse Chr 6, linked to the Cftr and Cpa genes. Comparative mapping of human MDK and HBNF employing species-specific polymerase chain reaction (PCR) primers and human monochromosomal somatic cell hybrids assigns MDK to human Chr 11 and HBNF to human Chr 7q32-qter. 相似文献
18.
Genetic mapping of the mouse ferritin light chain gene and 11 pseudogenes on 11 mouse chromosomes 总被引:2,自引:0,他引:2
We typed the progeny of two sets of genetic crosses to determine the map locations for loci containing sequences related
to the ferritin light chain (Ft11) gene. Twelve loci were positioned on 11 different chromosomes. One of these genes mapped to a position on Chr 7 predicted
to contain the expressed gene on the basis of the previously determined position of the human homolog on 19q13.3-q13.4.
Received: 23 July 1997 / Accepted: 20 September 1997 相似文献
19.
Béatrice Drouet Luis Garcia Dominique Simon-Chazottes Marie Geneviève Mattei Jean-Louis Guénet Arnold Schwartz Gyula Varadi Martine Pinçon-Raymond 《Mammalian genome》1993,4(9):499-503
Using both chromosomal in situ hybridization and molecular techniques, we report the genetic localization of the gene coding for the alpha 1 subunit of the skeletal slow Ca2+ current channel/DHP receptor gene (Cchl1a3) on human Chromosome (Chr) 1 (1q31–1q32 region) and on mouse Chr 1 region (F-G). On the basis of single-strand conformation polymorphism (SSCP-PCR) analysis in an interspecific backcross, we have determined that the Cchl1a3=mdg (muscular dysgenesis) locus is very closely linked to the myogenin (Myog) locus. 相似文献
20.
Amy F. Eisener-Dorman Laura Grabowski-Boase Brian M. Steffy Tim Wiltshire Lisa M. Tarantino 《Mammalian genome》2010,21(5-6):231-246
Quantitative trait locus (QTL) mapping in the mouse typically utilizes inbred strains that exhibit significant genetic and phenotypic diversity. The development of dense SNP panels in a large number of inbred strains has eliminated the need to maximize genetic diversity in QTL studies as plenty of SNP markers are now available for almost any combination of strains. We conducted a QTL mapping experiment using both a backcross (N2) and an intercross (F2) between two genetically similar inbred mouse strains: C57BL/6J (B6) and C57L/J (C57). A set of additive QTLs for activity behaviors was identified on Chrs 1, 9, 13, and 15. We also identified additive QTLs for anxiety-related behaviors on Chrs 7, 9, and 16. A QTL on Chr 11 is sex-specific, and we revealed pairwise interactions between QTLs on Chrs 1 and 13 and Chrs 10 and 18. The Chr 9 activity QTL accounts for the largest amount of phenotypic variance and was not present in our recent analysis of a B6 × C58/J (C58) intercross (Bailey et al. in Genes Brain Behav 7:761–769, 2008). To narrow this QTL interval, we used a dense SNP haplotype map with over 7 million real and imputed SNP markers across 74 inbred mouse strains (Szatkiewicz et al. in Mamm Genome 19(3):199–208, 2008). Evaluation of shared and divergent haplotype blocks among B6, C57, and C58 strains narrowed the Chr 9 QTL interval considerably and highlights the utility of QTL mapping in closely related inbred strains. 相似文献