培养基组分及培养条件对蜡梅花粉萌发及花粉管生长的影响

采用液体培养法研究不同培养基组分和培养条件对蜡梅花粉萌发和花粉管生长的影响。结果表明:(1)PEG-4000是蜡梅花粉离体培养所必需的培养基成分,当培养基中无PEG-4000时,花粉不能正常萌发。(2)培养基内低浓度蔗糖对花粉萌发和花粉管的生长无显著影响,但随着蔗糖浓度的升高,则对花粉萌发和花粉管生长表现出强烈的抑制作用,且浓度越高,抑制效应越强。(3)培养基内其它组分分别在一定浓度范围(0～250g/L PEG-4000、0～50mg/L硼酸、0～30mg/L硝酸钙)内对花粉萌发及花粉管生长有促进作用,但超过上述高限值时则起抑制作用。(4)培养基内镁和钾的浓度对花粉萌发及花粉管生长影响不显著。研究表明,蜡梅最适花粉液体培养基组分为250g/L PEG-4000+50mg/L H3BO3+30mg/L Ca(NO3)2.4H2O,且在pH 5.5、温度15℃和600lx的光照培养条件下蜡梅花粉萌发和花粉管生长最佳。     

薄壳山核桃花粉离体萌发和花粉管生长特性研究

以6年生薄壳山核桃优良品种‘金华’的新鲜花粉为试材,采用离体培养法,研究了不同复水时间、不同培养基组分、不同培养温度及培养时间对薄壳山核桃花粉萌发和花粉管生长的影响。结果显示:(1)薄壳山核桃花粉萌发试验前进行4h复水处理可显著提高萌发率,萌发率可达51.78%,为对照(2.51%)的20.68倍。(2)蔗糖、H3BO3、Ca(NO3)2·4H2O在一定浓度范围内均具有促进花粉萌发和花粉管生长的作用,但浓度过高则起抑制作用。(3)正交试验结果经实验验证表明,薄壳山核桃花粉萌发和花粉管生长的最适培养基为20%蔗糖+0.02%~0.03%H3BO3+0.05%Ca(NO3)2·4H2O,最佳培养条件为25℃下恒温培养24h,此时的花粉萌发率高达74.46%,花粉管平均长度为258.84μm。     

不同培养条件对黄连木花粉萌发和花粉管生长的影响

以黄连木花粉为试材,采用离体培养法研究了培养基组分和植物生长调节物质对黄连木花粉萌发和花粉管生长的影响.结果表明:花粉萌发和花粉管生长的适宜蔗糖浓度为15%,适宜培养温度为25℃;该培养条件下,花粉萌发率和花粉管长度分别达最大值63.3%和412.1 μm.硼酸、赤霉素(GA3)和吲哚乙酸(IAA)在一定浓度范围内,可以促进黄连木花粉萌发和花粉管生长,浓度过高时起抑制作用;最适宜黄连木花粉萌发和花粉管生长的硼酸浓度、赤霉素(GA3)和吲哚乙酸(IAA)浓度分别为100、50和15 mg/L.     

培养基组分及pH值对梨花粉萌发和花粉管生长的影响

采用花粉液体培养法研究不同培养基组分和pH值对梨花粉萌发和花粉管生长影响.结果表明:培养基内硼酸、氯化钙、PEG-4000、蔗糖、葡萄糖、麦芽糖、山梨醇、果糖在一定浓度范围内,对花粉萌发及花粉管生长起促进作用,但超过一定浓度时起抑制作用;最适的培养基组分为:30mmol/LMES、0.01%硼酸、0.03%CaCl2·2H2O、15%PEG-4000、5%果糖 或5%山梨醇、10%蔗糖 ,最适的pH值为6.5.在该培养基内梨花粉萌发率约为59.2%,花粉管生长长度为966.3μm.     

六种杜鹃花属植物花粉活力测定方法的比较研究

选取睫毛杜鹃(Rhododendron ciliatum)、多鳞杜鹃(Rhododendron polylepis)、薄皮杜鹃(Rhdodenron taronense)、映山红(Rhododendron simsii)、马银花(Rhododendron ovatum)和比利时杜鹃(Rhododendron hybridum)为研究对象,通过蔗糖、H3BO3、CaCl2单因子及L25( 53)正交试验对它们进行花粉萌发试验研究,比较I2-Kl染色法、TTC染色法、联苯胺染色法、MTT染色法4种花粉活力测定方法的不同.结果表明:蔗糖、H3BO3、CaCl2及3因子交互效应对杜鹃花花粉萌发有显著影响.适宜的离体培养基配方依杜鹃花种类不同而不同,睫毛杜鹃为:蔗糖150 g/L +H3BO3 0 mg/L +CaCl2 50 mg/L;映山红为:蔗糖100 g/L +H3BO3 100 mg/L +CaCl20 mg/L;马银花为:蔗糖50 g/L+ H3BO3 200 mg/L +CaCl2 0 mg/L;比利时杜鹃为:蔗糖150 g/L+ H3BO3100 mg/L +CaCl2 0 mg/L.MTT染色法是简单快速测定杜鹃花花粉活力的最适染色法.     

桔梗花粉萌发与花粉管生长研究

以2年生桔梗植株为材料,采用液体培养法研究了培养基种类、PEG、蔗糖、pH以及培养温度、培养时间对桔梗花粉离体萌发生长的影响,结果表明:(1)浓度为100~150 g.L-1的PEG可显著促进桔梗花粉萌发和花粉管的生长;200~250 g.L-1PEG显著促进花粉萌发,但对花粉管生长的作用不显著。(2)100 g.L-1的蔗糖有利于花粉萌发和花粉管生长,高浓度蔗糖(200 g.L-1)有明显抑制作用;(3)桔梗花粉离体萌发和花粉管生长的适宜培养基为ME3+BK+10%蔗糖+150 g.L-1PEG(pH5.8);(4)25~40℃条件下桔梗花粉均可较好萌发,以30℃培养1.5 h为最佳培养条件。     

巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究

通过研究接种量、激素配比、糖浓度、培养基种类对巫山淫羊藿悬浮培养细胞生长及其愈伤组织黄酮类含量的影响,建立了巫山淫羊藿细胞悬浮培养的技术体系.结果表明:巫山淫羊藿愈伤组织细胞悬浮培养在B5基本培养基中并附加1.0 mg·L-12,4-D和0.2 mg· L-1BA,蔗糖浓度40 g·L-1,接种量每30 mL为鲜重2 ...     

凯特杏花粉的离体培养及影响因子分析

采用离体培养法,在不同培养基组分含量、pH值、温度及植物生长调节物质的培养条件下,对凯特杏(Prunus armeniacaL.cv Katy)花粉的离体萌发和花粉管生长状况进行观察研究。结果表明:(1)凯特杏花粉离体萌发及花粉管生长的适宜培养基为20%蔗糖 0.04%硼酸 0.01?Cl2,最适pH为6.0,最适温度为20℃,培养20 h后,花粉的萌发率达68.76%,花粉管长度达1 083.53μm。(2)不同植物生长调节物质对花粉萌发和花粉管生长作用不同,赤霉素浓度为5~8 mg/L、矮壮素浓度为10~150 mg/L、多效唑浓度为5~10 mg/L时对凯特杏花粉萌发和花粉管生长都有促进作用,但国光丁酰肼对凯特杏花粉萌发和花粉管生长均有抑制作用。     

植物生长调节物质对丰水梨花粉萌发和花粉管生长的影响

采用花粉液体培养法研究了植物生长调节物质对梨花粉萌发和花粉管生长的影响,结果表明：较低浓度的赤霉素、三十烷醇、吲哚乙酸及2,4-D均能促进花粉萌发和花粉管生长,而超过一定浓度时却起抑制作用,最适宜于花粉萌发和花粉管生长的赤霉素浓度为50～300mg／L,三十烷醇为3～100mg／L,吲哚乙酸为5～25mg／L,2,4-D为5～10mg／L。萘乙酸对花粉萌发和花粉管生长有抑制作用,抑制程度随培养基内其浓度的增大而加强。多效唑和脱落酸对花粉萌发有抑制作用,其抑制程度随浓度的上升而增强,但他们对花粉管生长却有促进怍用,其最适宜于花粉管生长的浓度分别为400mg／L和60mg／L,超过此浓度后,促进作用又有所下降,甚至出现抑制作用,如多效唑浓度达到1000mg／L时,能强烈地抑制花粉管生长。     

楸树等4种梓属树种花粉离体培养条件的研究

为筛选出楸树(Catalpa bungei C. A. Mey. )等4个树种花粉离体培养的适宜条件,以花粉萌发率和花粉管长度为指标,研究了培养时间、培养温度、液体培养基pH值、蔗糖浓度和PEG-4000浓度对2个楸树花粉样品(CB-1和CB-2)及滇楸[C. fargesii Bur. f. duclouxii (Dode) Gilmour]、黄金树[C. speciosa (Warder ex Barney)Engelmann]和梓树(C. ovata G. Don)花粉离体萌发的影响.实验结果显示,不同培养时间对楸树的花粉萌发率和花粉管长度均有极显著影响,培养至6 h时花粉萌发率最高(91.0%),培养至6～7 h时花粉管长度达到最长,最适培养时间为6 h.培养温度、液体培养基pH值、蔗糖浓度和PEG-4000浓度对4个树种花粉萌发率和花粉管长度均有明显影响.在19 ℃～36 ℃范围内,较低或较高的培养温度均对花粉萌发有一定的抑制作用,适宜各树种花粉离体萌发的培养温度为24 ℃～28 ℃;供试的4个树种花粉适宜在弱酸性环境下萌发和生长,适宜的液体培养基pH值为5.0～5.6;在蔗糖浓度为15～25 g·L-1的液体培养基中,花粉萌发率及花粉管长度均达到最大值;液体培养基中添加不同浓度PEG-4000,在较短的培养时间(3 h)内明显抑制花粉萌发和花粉管生长,但培养时间延长至6 h,添加5～25 g·L-1 PEG-4000对花粉萌发有一定促进作用,适宜的PEG-4000浓度为20 g·L-1.结果表明,不同树种甚至同一树种不同种源适宜的花粉离体培养条件有一定的差异,应根据种类或种源进行适当的调整.     

燕山南部花粉散布特征

燕山南部花９粉用布特性研究表明,空气中的花粉反映的是植物花期的季节性变化,表 粉是植物多年花粉散布的混合,冲积物花粉与空气中和表土花粉显著不同,是取样点上游表土花粉的混合,与取样点周围植被关系较小。因此,根据冲积物花粉恢复古植被,反映的是整个流域的植被面貌,而不是某一点的植被特征。     

The collection of pollen by bees

Bees require pollen for their reproduction and pollen comprises the basic larval food for bees. Most bees acquire pollen passively during flower visitation, but female bees may also collect pollen actively with the aid of various structural and behavioral adaptations. Most bees have evolved adaptations to concentrate pollen into discrete loads and transport pollen back to their nests. The various structural and behavioral adaptations of female bees for acquiring and transporting pollen are the basis of this review.     

Birch pollen production,transport and deposition for the period 1984–1993 at Kevo,northernmost Finland

Summary Betula pollen production and flowering, pollen transport and pollen deposition are considered for the mountain birch region of northern Finland for the ten-year period 1984–1993. The most abundant flowering year was 1989 and, after that, 1985. In these years the highest values were also recorded for pollen in the air. There is a significant correlation between the amount of pollen released and the thermal sum of the previous year. In terms of pollen deposition the peak years were 1989 and 1986. The correlation between the amount of pollen in the air and that being deposited on the ground is also statisticaly significant. It is evident that some birch pollen is already present in the air before local flowering begins and that, in some years, this non-local pollen can account for more than 20% of the yearly total. This preflowering proportion was highest in 1985 and 1993, the latter being a prolific flowering year in the south of the country. There is a clear relationship between the proportion of the non-local pollen in the air and the proportion ofBetula pubescens/pendula type pollen deposited on the ground and, conversely, there is a significant correlation between the amount of local pollen in the air and the amount ofBetula tortuosa plusB. nana type pollen being deposited on the ground. The questions both of the viability of this long-distance pollen and of its ability to cause allergic reactions are considered. An assessment is also made of the degree to which fossil birch pollen assemblages can be realistically interpreted in terms of local vegetation if a varying proportion of the same pollen type is non-local.     

City spore concentrations in the European Economic Community (EEC). VI. Poaceae (Grasses), 1982–1986

Summary Airborne grass-pollen concentrations in six cities in the EEC are compared, based on observations from five years, 1982–1986. Results show that there are quantitative differences both between the monitoring stations and between the years. Very provisionally, the average seasonal total of the European urban airborne grass-pollen concentration can be put at 4 to 5000 per m³ of air. Also qualitatively, regarding the seasonal fluctuations, there are differences between the stations, and between the years. Generally, the results confirm that June is the most typical grass-pollen month in northwestern Europe, whereas in mediterranean Europe May is the more prominent grass-pollen period. There seems to be little coherence between the starting dates of the grass-pollen season even at relatively nearby stations in northwestern Europe, suggestive for a great influence of the actual weather situation.     

Pollen monitoring in Perugia and information about aerobiological data

Summary The results of six years (1982–1987) of aerobiological study in Perugia (central Italy) are reported. During this period a total of 80 different types of pollen belonging to 52 families were identified in the Perugia atmosphere. The highest percentages of pollens were found in the mid-March to mid-July period. For main taxa principal periods of pollination were identified. The outlines for the weekly issue in a newspaper of the concentration pattern of the allergenic pollens are reported.     

Pollen distribution at elevations above 1000 m inSwitzerland

In Switzerland the concentration of allergenic pollendecreases with increasing elevation of the samplingsite of the Swiss pollen measuring network. The aim ofthis study was, to compare the pollen concentrationsof five different localities in the Swiss Alps and inthe Jura mountains, to get an idea of the differencesin the pollen abundance in altitudes above 1000 m. Theinvestigated localities are: La Chaux-de-Fonds(1040 m), Wiesen (1420 m), Davos (1600 m), Samedan(1705 m) and Gütsch (2287 m). These pollen sumswere compared to the Swiss plateau (Basel 273 m).Basel, La Chaux-de-Fonds and Wiesen show clearlyhigher pollen values than Davos, Samedan andGütsch. Pollen concentrations of Corylus,Alnus glutinosa-type and Fraxinus arereduced with increasing altitude and are only a smallproblem for allergies above 1000 m. Betulapollen are also reduced in the higher localities, butthere still occur many days with high pollenconcentrations. Especially Betula pollen frommedium range transport can play an important role forallergies in higher localities. The total grass pollensum does not decrease up to an altitude of 1500 m. Thegrass pollen concentration depends more on factorslike composition of the local grassland, land use andwind situation.Pollen concentrations at higher elevations isinfluenced by the local vegetation, a reduced pollenproduction, land use, topography, exposition, windvelocity and wind direction. These factors modify thepollen concentration considerably. Due to the complextopography of the Prealps and Alps, it is thereforenot possible to propose a general rule of pollenreduction with increasing altitude.     

Pollen starch reserves in tomato relatives: Ecophysiological implications

The presence or absence of starch in microspore development and in pollen grains was recorded in eleven wild tomato species (Solanum sect. Lycopersicon) and two close relatives (S. lycopersicoides and S. sitiens). In all the species starch started to accumulate in the early microspore bicellular stage and continued until the cytoplasm was filled. At flower anthesis, pollen grains were mostly starchless in the wild tomatoes, except in S. pennellii, which had starchy pollen. Starchy pollen is also present in the two related species. The latter two species had larger pollen grains and grow in drier environments than the other species. The heterogeneity of pollen starch content among all these species, supposed to have the same pollination mechanism, is a new finding supporting the idea that starch content and pollination mechanism do not necessarily influence each other. The presence of starchy pollen in the self‐incompatible species, which grow in the driest environments, raises questions regarding the relationship between carbohydrates content and pollen survival.     

A pilot study on pollen representation of mountain valley vegetation in the central Alps

The relative pollen productivity (RPP) of Pinus cembra, Larix, Picea, Gramineae, and Cyperaceae was estimated for the Upper Engadine area, an inner-alpine sub-continental valley in SE Switzerland lying above 1800 m a.s.l. The influx of pollen originating from lowlands was assumed constant between the investigated sites and could thus be used to establish relative pollen accumulation rates (rPAR) as a basis for further calculations. Wind above the canopy was assumed as the major mode of pollen transport and the effect of different wind speeds was tested. In order to evaluate the results pollen percentage values were simulated and compared with the empirical data. The method is sufficiently accurate for evaluation of spatially-explicit vegetation models, although the low number of sites eludes robust statistical testing.     

沈阳地区气传致敏花粉探讨

本文报道在沈阳地区1965—1985年间于空气中间断收集大气花粉5年,共收花粉玻片1100张,花粉45780粒,属于24科37属或种。其中9种(或属)超过千粒,为优势种类花粉。每年沈阳市大气中花粉出现两个高峰,一个为春季(四、五月),另一个为夏秋季(七八月)。通过长期观察发现,花粉种类和数量每年虽有不同,伹差异不大,唯有豚草花粉消长明显。大气中花粉种类及数量的变化往往受到气温、国家有关绿化政策以及社会风气的影响。花粉与过敏症关系十分密切,我们采用17种花粉制成浸液为病人皮试和治疗。皮试结果阳性率最高的为蒿属花粉,其次为其它夏秋花粉,春季花粉阳性率不高,致敏性不强。用花粉浸液对过敏症者进行免疫治疗,收到一定的疗效。