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1.
在低于和高于400 μmol·m·s-1的光照下,苹果(Malus pumila Mill.cv.Tengmu No.1/Malus hupehensisRehd.)叶片稳态荧光(Fs)随光强增加而分别呈现升高和降低的变化趋势,但是光适应下的最大荧光(Fm′)和最小荧光(Fo′)在整个光照范围内一直表现为下降,而且更多的光系统Ⅱ(PS Ⅱ)反应中心(RC)处于关闭状态((Fs-Fo′)/(Fm′-Fo′)).这表明在高于400 μmol·m-2·s-1的光照下,Fs降低并不是PSⅡ反应中心关闭的结果,而主要是光能从PSⅡ聚光天线(LHCⅡ)向PSⅡ RC的传递减少所导致的.在引入状态转换抑制剂NEM的情况下,Fs在400 μmol·m-2·s-1至苹果叶片饱和光强700 μmol·m-2·s-1的范围内继续上升.另外,在叶黄素循环抑制剂DTT预处理下,Fs可在高于700 μmol·m-2·s-1的光照下继续升高.这些变化说明在饱和光强以下和以上的光照下,苹果叶片Fs变化分别受状态转换和叶黄素循环的影响.在NEM预处理下,PSⅡ表观光化学反应速率(P-rate)和光化学猝灭(qP)在600800 μmol·m-2·s-1光照下显著降低,与此同时,非光化学猝灭(qN)在600-800 μmol·m-2·s-1光照下轻微上升,而在800 μmol·m-2·s-1以上光照下略微下降.这些现象说明状态转换对于苹果叶片在低于饱和光强的光照下主要起光化学作用,在高于饱和光强光照下主要起非光化学作用.  相似文献   

2.
研究了两系超高产杂交稻(Oryza sativa L.)"两优培九"和"华安3号"以及多年来大面积推广的三系杂交稻"汕优63"剑叶的PSⅡ活性和叶黄素循环对强光胁迫及其恢复进程的响应.结果表明,在2 000 μmol photons*m-2*s-1的强光胁迫下,3个杂交稻的PSⅡ光化学最大效率(Fv/Fm)、开放的PSⅡ反应中心捕获激发能效率(Fv′/Fm′)和PSⅡ的实际光化学转能效率(ΦPSⅡ)都随着光抑制进程而下降,其中以"汕优63"下降的幅度最大.光抑制过程中,杂交稻叶黄素循环的紫黄素(V)组分迅速下降,与此同时,脱环氧化组分环氧玉米黄素(A)和玉米黄素(Z)迅速积累,而超高产杂交稻"两优培九"和"华安3号"的A和Z的积累速度大大高于"汕优63".伴随A和Z的快速积累,它们的叶黄素循环的脱环氧化状态(DES)迅速上升,并在半小时左右基本达到最大值,其中"两优培九"和"华安3号"DES的上升速率仍然较"汕优63"高.光抑制处理结束后,转移到弱光(70 μmol photons*m-2*s-1)条件下恢复过程中,两个超高产杂交稻的Fv/Fm、Fv′/Fm′和ΦPSⅡ的恢复速率和恢复水平都高于"汕优63".同时,它们的叶黄素循环组分V、A、Z以及DES都逐渐恢复,但"两优培九"和"华安3号"的恢复速率和恢复水平仍然要优于"汕优63".以上结果说明,超高产杂交稻"两优培九"和"华安3号"较对照品种"汕优63"具有更强的抗光抑制及光保护能力,同时在光抑制结束后又能够更迅速地恢复光合功能,较强的抗光抑制能力和较高的恢复能力可能是超高产杂交稻高产的重要生理基础之一.  相似文献   

3.
烟草花叶病毒对烟草叶片光合特征和POD表达的影响   总被引:2,自引:0,他引:2  
以烤烟(Nicotiana tabacum L.)品种'中烟5号'为实验材料,对烟草健康株与感染烟草花叶病毒(TMV)株的叶绿素、光合速率、光合速率对光强的响应曲线、光暗反应荧光特征、POD活性及其表达等进行研究,以探讨TMV感染对烟草植株生理生态特征的影响.结果显示:病株的叶绿素a(Chl a)和叶绿素b(Chl b)含量显著低于健康株,但Chl a/Chl b值基本相同;病株暗中初始荧光(F0)、暗中最大荧光(Fm)、暗中可变荧光(Fv)、光下初始荧光(F0′)、光下最大荧光(Fm′)、光下可变荧光(Fv′)、非光化学猝灭系数(NPQ)、PSⅡ捕光效率(Fv′/Fm′)、PSⅡ实际光化学效率(ФPSⅡ)及光饱和点显著低于健康株;净光合速率在光强较大(>1 500 μmol·m-2·s-1)时病株比健康株低,光强适中(1 500 μmol·m-2·s-1左右)时两者相差不大,光强较弱(<1 500 μmol·m-2·s-1左右)时病株比健康株高;病株叶片的过氧化物酶(POD)活性显著升高,POD同工酶中一些大分子量蛋白分子表达量加大.研究表明,感染TMV使烟草植株对光抑制更为敏感,叶片的荧光激发能力和热耗散能力下降,PSⅡ反应中心捕光效率和光化学反应效率降低,光合电子传递能力和碳同化能力受到抑制;POD活性提高和表达量增加可能是诱导烟草抗病性的一个关键生理过程.  相似文献   

4.
以小麦品种矮抗58为材料,采用0.3 mmol/L水杨酸(SA)溶液预处理灌浆期小麦叶片,以水预处理为对照,进行3种不同的光温处理:适宜温度中等光强(25℃,600 μmol m-2 s-1)2h、高温强光(38℃,1600μmol m-2 s-1)2h、高温强光2h后置于适宜温度中等光强下恢复3h.测定不同光温条件下,小麦叶绿体的Deg1蛋白酶、D1蛋白和PSⅡ功能的变化及SA的调节效应.结果表明,高温强光胁迫导致Deg1蛋白酶和D1蛋白降解,PSⅡ功能发生可逆损伤.与对照相比,水杨酸预处理不仅能够抑制高温强光下小麦叶绿体Deg1蛋白酶和D1蛋白的降解,维持较高的PSⅡ原初光化学效率(Fv/ Fm)、实际光化学效率(φPSⅡ)、电子传递速率和净光合速率(Pn),而且加快回到非逆境下PSⅡ功能的恢复.  相似文献   

5.
高、低温胁迫对牡丹叶片PSⅡ功能和生理特性的影响   总被引:1,自引:0,他引:1  
以牡丹‘肉芙蓉’离体叶片为试材,以25 ℃为对照,研究了强光(1400 μmol·m-2·s-1)下高温(40℃)和低温(15℃)处理对牡丹叶片PSⅡ光化学活性和生理特性的影响.结果表明:随处理时间的延长,各处理叶片的PSⅡ最大光化学效率(Fv/Fm)、PSⅡ实际光量子效率(φPsⅡ)和光下开放的PSⅡ反应中心激发能捕获效率(Fv’/Fm’)均持续降低.暗恢复4h后,对照和15℃处理叶片的Fv/Fm基本上完全恢复,而40℃处理叶片仅恢复到处理前的75.5%,即使15 h后也不能完全恢复;强光下40℃处理使PSⅠ和PSⅡ间的激发能分配严重偏离平衡状态.强光下40 ℃处理抑制了超氧化物歧化酶活性,加剧了O2、H2O2、丙二醛的产生,导致叶绿素和可溶性蛋白含量不断下降.说明强光下40℃高温胁迫对牡丹叶片光合机构造成了不可逆的破坏,而15℃低温处理对其光合机构的影响相对较弱.  相似文献   

6.
在低于和高于400 μmol.m-2.s-1的光照下,苹果(Malus pumila Mill. cv. Tengmu No.1/Malus hupehensisRehd.)叶片稳态荧光(Fs)随光强增加而分别呈现升高和降低的变化趋势,但是光适应下的最大荧光(Fm′)和最小荧光(Fo′)在整个光照范围内一直表现为下降,而且更多的光系统Ⅱ(PSⅡ)反应中心(RC)处于关闭状态((Fs-Fo′)/(Fm′-Fo′))。这表明在高于400 μmol.m-2.s-1的光照下,Fs降低并不是PSⅡ反应中心关闭的结果,而主要是光能从PSⅡ聚光天线(LHCⅡ)向PSⅡ RC的传递减少所导致的。在引入状态转换抑制剂NEM的情况下,Fs在400 μmol.m-2.s-1至苹果叶片饱和光强700 μmol.m-2.s-1的范围内继续上升。另外,在叶黄素循环抑制剂DTT预处理下,Fs可在高于700 μmol.m-2.s-1的光照下继续升高。这些变化说明在饱和光强以下和以上的光照下,苹果叶片Fs变化分别受状态转换和叶黄素循环的影响。在NEM预处理下,PSⅡ表观光化学反应速率(P-rate)和光化学猝灭(qP)在600-800 μmol.m-2.s-1光照下显著降低,与此同时,非光化学猝灭(qN)在600-800 μmol.m-2.s-1光照下轻微上升,而在800 μmol.m-2.s-1以上光照下略微下降。这些现象说明状态转换对于苹果叶片在低于饱和光强的光照下主要起光化学作用,在高于饱和光强光照下主要起非光化学  相似文献   

7.
25~30℃和30 μmol m~(-2)s~(-1)光下培养的黄瓜幼苗,在黑暗下经 1~7℃处理24h或5℃处理24~72h,光合电子传递活性受不同程度的抑制;其抑制部位主要在PSⅡ氧化侧;随温度的降低和时间的延长,抑制部位可发展至PSⅡ及之后的电子递体上,但尚未影响PSⅠ的活性。160μmol m~(-2)s~(-1)的光强加重低温对电子传递活性的抑制,光强越高,则加重的程度越高;抑制部位从PSⅡ氧化侧发展至PSⅡ反应中心以及PSⅠ。  相似文献   

8.
ALA对遮荫条件下西瓜幼苗强光抑制的保护效应   总被引:3,自引:0,他引:3  
以遮荫生长的盆栽西瓜幼苗为材料,研究了100mg/L5-氨基乙酰丙酸(5-aminolevulinic acid,ALA)处理对暗适应叶片转入强光下叶绿素荧光特性的影响.结果显示,遮荫能显著提高暗适应叶片的Fo,降低Fv/Fm和Fv/Fo;正常光照下生长的植株叶片暗适应后转入1500μmol·m-2·s-1作用光强下5min的ΦPSⅡ、qP和Pc分别为0.176、0.399和0.180,约为600μmol·m-2·s-1作用光强下的62%、72%和64%;而遮荫下生长的幼苗叶片暗适应后转入1500μmol·m-2·s-1作用光强下的ΦPSⅡ、qP和Pc分别为0.089、0.301和0.089,仅为600μmol·m-2·s-1作用光强的40%、66%和40%;遮荫还显著降低西瓜叶片暗适应后转入强光1500μmol·m-2·s-1的PCR和qL,同时提高L(PFD).ALA处理能提高遮荫西瓜幼苗叶片PCR、Pc、qL和Hd等荧光参数,降低Ex、ΦNO和L(PFD);SOD活性抑制剂DDC处理降低PCR、Pc、qL和Hd等荧光参数,而ALA处理可以逆转DDC的抑制效应;ALA处理能提高西瓜幼苗叶片SOD、POD和APX活性.研究发现,遮荫导致西瓜幼苗光抑制程度加重,ALA通过增强PSⅠ附近SOD等抗氧化酶活性,促进水-水循环,增加热耗散,减轻光抑制,提高西瓜幼苗叶片的光化学效率,从而对强光下的光合作用起到保护作用.  相似文献   

9.
砂仁叶片光破坏的防御   总被引:12,自引:6,他引:12  
报告了生长于热带林窗向阳处砂仁叶片叶绿素荧光参数的日变化,及阻止卷叶和用二硫苏糖醇(DTT)处理对它的影响.受强光照射,砂仁叶片迅速卷起.在雾天上午光强还相当弱(低于100μmol m-2s-1)时,砂仁叶片就发生光抑制,中午最重,下午当光强减弱时,光抑制逐渐得到缓解.其热耗散(qN、NPQ)随光强的升高而增加,且下午仍在缓慢增加.阻止卷叶使强光下砂仁叶片光抑制加剧,F0、NPQ升高.DTT处理也使光抑制加剧,F0升高,且使PSⅡ反应中心发生可逆失活.夜间2300各处理的荧光参数基本恢复.卷叶、叶黄素循环和PSⅡ可逆失活3种保护机制在同种植物中依次启动的现象尚属少见.  相似文献   

10.
已有研究表明叶绿体内有200种蛋白酶,然而,多数蛋白酶的作用机制尚不清楚,尤其哪些蛋白酶参与了D1蛋白周转.其中Deg2蛋白酶体外实验证明,其参与了光损伤D1蛋白的的初步剪切.为了进一步研究Deg2蛋白酶在植物体内的作用机制,我们筛选了拟南芥Deg2蛋白酶功能缺陷型突变体.在120 μmol·m-2·s-1光照生长条件下,deg2突变体与野生型的生长曲线基本一致;在进一步的高光胁迫(1 800 μmol·m-2·s-1)处理及相同的光胁迫处理条件下,无论林可霉素存在与否,突变体PSⅡ的最大光化学效率(Fv/Fm)都和野生型没有区别;利用蛋白免疫印迹实验同样证明了光损伤D1蛋白的降解速度在deg2突变体和野生型之间也没有明显区别.我们认为Deg2蛋白酶在光抑制情况下对于光损伤D1蛋白的降解以及PSⅡ的修复不是必需的.  相似文献   

11.
Exposure of algae or plants to irradiance from above the light saturation point of photosynthesis is known as high light stress. This high light stress induces various responses including photoinhibition of the photosynthetic apparatus. The degree of photoinhibition could be clearly determined by measuring the parameters such as absorption and fluorescence of chromoproteins. In cyanobacteria and red algae, most of the photosystem (PS) II associated light harvesting is performed by a membrane attached complex called the phycobilisome (PBS). The effects of high intensity light (1000-4000 micromol photons m(-2) s(-1)) on excitation energy transfer from PBSs to PS II in a cyanobacterium Spirulina platensis were studied by measuring room temperature PC fluorescence emission spectra. High light (3000 micromol photons m(-2) s(-1)) stress had a significant effect on PC fluorescence emission spectra. On the other hand, light stress induced an increase in the ratio of PC fluorescence intensity of PBS indicating that light stress inhibits excitation energy transfer from PBS to PS II. The high light treatment to 3000 micromol photons m(-2) s(-1) caused disappearance of 31.5 kDa linker polypeptide which is known to link PC discs together. In addition we observed the similar decrease in the other polypeptide contents. Our data concludes that the Spirulina cells upon light treatment causes alterations in the phycobiliproteins (PBPs) and affects the energy transfer process within the PBSs.  相似文献   

12.
低温弱光胁迫对日光温室栽培杏树光系统功能的影响   总被引:4,自引:0,他引:4  
以温室栽培的金太阳杏为材料,测定了金太阳杏叶片光合速率(Pn)、光系统Ⅱ(PSⅡ)光下实际光化学效率(ΦPSⅡ)、光化学猝灭系数(qP)和开放的PSⅡ反应中心的激发能捕获效率(Fv/Fm), 探讨了低温弱光(7 ℃、200 μmol·m-2·s-1 PFD)对叶片光系统Ⅰ(PSⅠ)和PSⅡ的抑制作用.结果表明:温室栽培的金太阳杏叶光合作用的最适温度在25 ℃左右.光下7 ℃的低温可使叶片净光合速率(Pn)大幅下降,造成激发压(1-qP)增大,进而引起光抑制.低温弱光条件使PSⅠ和PSⅡ功能受到破坏,与单纯低温胁迫(7 ℃,黑暗)处理相比,经低温、弱光(7 ℃, 200 μmol·m-2·s-1PFD)胁迫2 h后,PSⅠ活性下降了28.26%,而PSⅡ最大光化学效率(Fv/Fm)没有发生显著变化,表明低温弱光条件下PSⅠ比PSⅡ 更易发生光抑制.  相似文献   

13.
Reversible phosphorylation and turnover of the D1 protein in vivo were studied under low-temperature photoinhibition of pumpkin leaves and under subsequent recovery at low light at 4 °C or 23 °C. The inactivation of PS II and photodamage to D1 were not enhanced during low-temperature photoinhibition when compared to that at room temperature. The PS II repair cycle, however, was completely blocked at 4 °C at the level of D1 degradation. Both the recovery of the photochemical activity of PS II and the degradation of the damaged D1 protein at low light at 23 °C were delayed about 1 hour after low-temperature photoinhibition, suggesting that in addition to the decrease in catalytic turnover of the enzyme, the protease was specifically inactivated in vivo at low temperature. The effect of low temperature on the other regulatory enzymes of PS II repair, protein kinase and phosphatase [Rintamäki et al. (1996) J Biol Chem 271: 14870-14875] was variable. The D1 protein kinase was operational at low temperature while dephosphorylation of the D1 protein seemed to be completely inhibited during low temperature treatment. Under subsequent recovery conditions at low light and 23 °C, the high phosphorylation level of D1 was sustained in leaf discs photoinhibited at low temperature, despite the recovery of the phosphatase activity. This high phosphorylation level of D1 was due to the persistently active kinase. The D1 kinase, previously shown to get activated by reduction of plastoquinone, was, however, found to be maximally active already at relatively low redox state of the plastoquinone pool. We suggest that phosphorylation of PS II centers increases the stability of PS II complexes and concomitantly improves their survival under stress conditions.  相似文献   

14.
When 23 °C-grown potato leaves (Solanum tuberosum L.) were irradiated at 23 °C with a strong white light, photosynthetic electron transport and Photosystem-II (PS II) activity were inhibited in parallel. When the light treatment was given at a low temperature of 3 °C, the photoinhibition of photosynthesis was considerably enhanced, as expected. Surprisingly, no such stimulation of photoinhibition was observed with respect to the PS II function. A detailed functional analysis of the photosynthetic apparatus, using in-vivo fluorescence, absorbance, oxygen and photoacoustic measurements, and artificial electron donors/acceptors, showed a pronounced alteration of PS I activity during light stress at low temperature. More precisely, it was observed that both the pool of photooxidizeable reaction center pigment (P700) of PS I and the efficiency of PS I to oxidize P700 were dramatically reduced. Loss of P700 activity was shown to be essentially dependent on atmospheric O2 and to require a continued flow of electrons from PS II, suggesting the involvement of the superoxide anion radical which is produced by the interaction of O2 and the photosynthetic electron-transfer chain through the Mehler reaction. Mass spectrometric measurements of O2 exchange by potato leaves under strong illumination did not reveal, however, any stimulation of the Mehler reaction at low temperature, thus leading to the conclusion that O2 toxicity mainly resulted from a chilling-induced inhibition of the scavenging system for O2-radicals. Support for this interpretation was provided by the light response of potato leaves infiltrated with an inhibitor (diethyldithiocarbamate) of the chloroplastic Cu-Zn superoxide dismutase. It was indeed possible to simulate the differential inhibition of the PS II photochemical activity and the linear electron transport observed during light stress at low temperature by illuminating at 23 °C diethyldithiocarbamate-poisoned leaves. The experimental data presented here suggests that (i) the previously reported resistance of PS I to photoinhibition damage in-vivo is not an intrinsic property of PS I but results from efficient protective systems against O2 toxicity, (ii) PS I is photoinhibited in chilled potato leaf due to the inactivation of this PS I defence system and (iii) PS I is more sensitive to superoxide anion radicals than PS II.Abbreviations PS - Photosystem - E - Emerson enhancement - open p and P maximal and actual quantum yields of PS II photochemistry - DDC - diethyldithiocarbamate - QA and QB - primary and secondary (quinone) electron acceptors of PS II - P680 and P700 - reaction center pigments of PS II and PS I, respectively - SOD - superoxide dismutase  相似文献   

15.
温州蜜柑叶片光系统反应中心光能分配的变化   总被引:8,自引:4,他引:4  
为深入了解果树光化学反应中心光能分配的状况,以柑橘为试材,采用调制荧光法对叶片光系统在高光强和低光强下的状态转换进行了研究.结果表明,光系统在100μmol·m^-2·s^-1的低光强下,由于QA的还原使PQ库处于还原状态,导致光能由PSⅡ转向PSⅠ分配,光系统处于状态2;在1000μmol·m^-2·s^-1的高光强下,PQ库无法得到电子而处于氧化状态,导致光能分配由PSⅠ转向PSⅡ,光系统处于状态1,叶片经磷酸酯酶抑制剂NaF处理后,光系统从高光强下状态2到状态1的转换受到抑制,高光强下过多的光能由PSⅠ向PSⅡ分配是导致PSⅡ光破坏的重要原因.  相似文献   

16.
Light-dependent reduction of the plastoquinone pool regulates the activity of the thylakoid-bound protein kinase which phosphorylates the light harvesting chlorophyll a,b-protein complex (LHC II) and regulates energy distribution between photosystems II (PS II) and I (Staehelin, L. A., and C. J. Arntzen, 1983, J. Cell Biol., 97:1327-1337). Since reduction of plastoquinone by PS II is abolished in photoinhibited thylakoids due to loss of the secondary electron acceptor QB protein (Kyle, D. J., I. Ohad, and C. J. Arntzen, 1984, Proc. Natl. Acad. Sci. USA, 81:4070-4074), it was of interest to examine the activity of the LHC II protein kinase system during photoinhibition and recovery of PS II activity. The kinase activity was assessed both in vivo and in vitro in Chlamydomonas cells exposed to high light intensity (photoinhibition) and recovery at low light intensity. The kinase activity was progressively reduced during photoinhibition and became undetectable after 90 min. The inactive LHC II-kinase system could not be reactivated in vitro either by light or by reduction of the plastoquinone pool following addition of reduced duroquinone (TMQH2). The LHC II polypeptides were dephosphorylated in vivo when cells, prelabeled with [32P]orthophosphate before exposure to high light intensity, were transferred to photoinhibiting light in the presence of [32P]orthophosphate. In vivo recovery of the LHC II-kinase activity, elicited by the addition of TMQH2 to the assay system, did not require restoration of QB-dependent electron flow or de novo protein synthesis, either in the cytoplasm or in the chloroplast. Mild sonication of thylakoids isolated from photoinhibited cells restored the ability of the LHC II protein kinase system to be activated in vitro by addition to TMQH2. Restoration of the light-activated LHC-II kinase required recovery of QB-dependent electron flow. At the structural level, photoinhibition did not affect the ratio of grana/stroma thylakoids. A reduction of approximately 20% of the 11-17-nm intramembrane particles and an equivalent increase in the number of 6-10.5-nm particles was observed on the E-fracture faces of stacked thylakoid membranes. Similar but smaller changes were observed also on the E-fracture faces of unstacked thylakoid membranes (more 10-14-nm and less 6-9-nm particles) and P-fracture faces of stacked thylakoid membranes (more 6-8- and less 9.5-13-nm particles). All these structural changes were reversed to normal values during recovery of PS II activity.(ABSTRACT TRUNCATED AT 400 WORDS)  相似文献   

17.
研究了不同浓度NO3-胁迫对黄瓜幼苗叶片光合速率、PSⅡ光化学效率及光能分配的影响.结果表明,当NO3-浓度较低时(14~98 mmol·L-1),适当增加NO3-浓度,可增强黄瓜幼苗叶片对光的捕获能力,促进光合作用.随着NO3-浓度的进一步增加(140~182 mmol·L-1),PSⅡ光化学效率降低,电子传递受到抑制,净光合速率降低;吸收的光能中,通过天线色素的热耗散增加,用于光化学反应的能量降低,光化学效率下降.140和182 mmol·L-1 NO3-处理黄瓜幼苗叶片6 d后净光合速率(Pn)极显著下降,分别比对照降低了35%和78%;PSⅡ最大光化学效率(Fv/Fm)、天线转化效率(Fv’/Fm’)、实际光化学效率(ΦPSⅡ)、光化学猝灭系数(qP)均低于对照,非光化学猝灭(NPQ)高于对照,激发能在两个光系统间的分配不平衡性(β/α-1)增大.高浓度NO3-处理的黄瓜幼苗叶片各荧光参数变化幅度比低浓度大.当光照增强时,高浓度NO3-胁迫下黄瓜幼苗叶片吸收的光能中应用于光化学反应的份额(P) 显著降低,天线热耗散的份额(D)显著增加. 天线热耗散是耗散过剩能量的主要途径.  相似文献   

18.
Jiang ZS  Sun XQ  Ai XZ  Wang ML  Bi HG  Wang HT 《应用生态学报》2010,21(8):2045-2050
Using 'Jinyou 3' cucumber seedlings as test materials, this paper studied their photosynthetic rate (P(n)), Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase (RCA) activities, and gene expression of Rubisco and RCA under optimal temperature and weak light (WL: 25 degrees C/18 degrees C, 100 micromol x m(-2) x s(-1)), suboptimal temperature and weak light (ST+WL: 18 degrees C/12 degrees C, 100 micromol x m(-2) x s(-1)), and low temperature and weak light (LT+WL: 10 degress C/5 degrees C, 100 micromol x m(-2) x s(-1)). Comparing with the control (25 degrees C/18 degrees C, 400 micromol x m(-2) x s(-1)), treatments WL, ST+WL, and LT+WL all led to a remarkable decrease in leaf area and dry matter mass. At initial stage, the P(n), Rubisco activity, rbcL and rbcS expression, RCA activity, and CsRCA expression in the three treatments declined by a big margin; 5-7 days later, these parameters tended to be less changed in treatment WL, ascended slowly in treatment ST+WL, and decreased continuously in treatment LT+WL. These results suggested that the photosynthetic apparatus of test cucumber seedlings could gradually adapt to weak light or suboptimal temperature and weak light. The Rubisco and RCA activities and the gene expression of Rubisco and RCA showed the similar responses to low temperature and weak light as the P(n), suggesting that the decline in Rubisco and RCA activities and gene expression in cucumber seedlings under low temperature and weak light could be the important reason leading to the decrease of P(n).  相似文献   

19.
低氧胁迫下黄瓜植株热耗散途径   总被引:5,自引:1,他引:4  
Jia YX  Sun J  Wang LP  Shu S  Guo SR 《应用生态学报》2011,22(3):707-712
采用营养液栽培,研究了低氧(营养液溶氧浓度为0.9~1.1 mg·L-1)胁迫下黄瓜幼苗光合作用热耗散与叶黄素循环的关系.结果表明:低氧胁迫下,黄瓜叶片PSⅡ的实际光化学效率(φPSⅡ)、饱和光强下的净光合速率(Pn)、表观量子效率(AQY)和PSⅡ的最大光化学效率(Fv/Fm)均显著降低,表明黄瓜植株的光合作用受到了光抑制;同时,光化学猝灭系数(qp)降低,而热耗散(NPQ)和天线耗散能量(D)的比值显著升高,说明黄瓜叶片热耗散增强;NPQ与叶黄素脱环氧化状态(DEPS)呈显著正相关,且两者均被抗坏血酸(AsA)所促进,被二硫苏糖醇(DTT)所抑制,说明低氧胁迫下,叶黄素循环是黄瓜植株光合作用热耗散的主要途径.  相似文献   

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