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1.
An asexual morph of a powdery mildew was found on Buddleja asiatica (Scrophulariaceae) in Nepal. The morphological traits of this powdery mildew strongly resemble those of Phyllactinia species (including Ovulariopsis) belonging to a phylogenetically basal group closely related to Leveillula species, but phylogenetic examinations and analyses clearly showed that this fungus pertains to Leveillula. Within the latter genus, it seems to be allied to L. duriaei, but clearly differs from this species in having conidiophores arising from superficial hyphae and narrower conidia with different length/width ratios. Therefore, the powdery mildew on B. asiatica is described as Leveillula buddlejae sp. nov.  相似文献   

2.
Coltriciella minuscula sp. nov. is described and illustrated from a specimen collected on Pinus merkusii from Bogor, Indonesia. This species is characterized by the size of its basidiocarp up to 4 mm in diam, with long hair on the stipe and with ornamented spores. Both morphological distinctiveness and phylogenetic separation based upon analyses of nrDNA ITS sequences support the establishment of this new species. Morphological dissimilarities between C. minuscula and closely related species are discussed.  相似文献   

3.
Pinus amamiana is an endangered Pinus species found only on Yakushima Island and Tanegashima Island, Japan. We surveyed remaining P. amamiana forests and found some sporocarps of Rhizopogon (Boletales), many species of which exhibit strict host specificity to a narrow range of Pinaceae trees and play critical roles in host establishment. Based on morphological characteristics and molecular phylogeny, here we describe Rhizopogon yakushimensis sp. nov. This new species belongs to a new clade, phylogenetically related to the subgenera Versicolores and Rhizopogon. We also confirmed its ectomycorrhizal association with P. amamiana by comparing rDNA ITS sequences between the sporocarps and ectomycorrhizal root tips.  相似文献   

4.
Species in the genus Gorgoderina Looss, 1902 are parasites of the urinary bladder of amphibians and include around 50 species described globally. Molecular data on species of the genus are scarce, as is the information of their life-cycle patterns. During a survey on the genetic characterization of the frog trematodes in the tropical rain forest of Los Tuxtlas, in the Gulf of Mexico slope of Mexico, specimens of two morphotypes of Gorgoderina were sampled from the Rio Grande leopard frog, Rana berlandieri. One of them represented an undescribed species which is described herein as Gorgoderina rosamondae n. sp., whereas the other one was morphologically very similar to an apparently widely distributed North American species, G. attenuata, which has been previously reported in the same geographical area. Specimens of both morphotypes were sequenced for two nuclear and one mitochondrial genes. Phylogenetic trees corroborated the distinction of the new species, and data on the internal transcribed spacer 2 revealed genetic differences between G. attenuata sequenced from frogs in USA and specimens of Gorgoderina sp. from Los Tuxtlas, indicating the possibility that they also represent an undescribed species. COI sequences showed high genetic divergence values between the new species and Gorgoderina sp. from Los Tuxtlas (8.63–9.99%). Additionally, COI sequences of the larval forms (sporocyst, cercariae and metacercariae) sampled in the same locality from their first and second intermediate hosts (Pisidium sp. and Agriogomphus tumens, respectively) showed conspecificity, and the 3 host life-cycle of the new species was elucidated.  相似文献   

5.
To compare morphological characters and phylogenetic placement between Japanese and European Russula, 32 specimens of 12 species were collected from Japanese subalpine forests and Northern Europe. Several sequences of nrDNA ITS region (ITS) of these Russula species were obtained. High homological similarities were shown between ITS sequences of several Russula samples collected from Japanese subalpine forests, Europe and North America. These facts show distribution of the same Russula species among these areas. From morphological observations and phylogenetic analyses, two same Russula species, R. velenovskyi, and R. decolorans are found in Japan, Europe and North America. Of these, R. velenovskyi collected from Mt. Fuji, Mt. Nyukasa and Mt. Tateshina in mountainous area of central Honshu is reported as a new Japanese record.  相似文献   

6.
A new species of Colletotrichum was described, based on morphology and phylogeny. The fungus was isolated in Madagascar from healthy leaves of Centella asiatica, in Mexico from wild native of Stylosanthes guianensis and in Colombia from Coffea arabica. The fungus differed from the currently related species in the genus by its longer and wider size of conidia. In potato dextrose agar medium supplemented with sterilized leaf powder of Ce. asiatica, the fungus produced fertile perithecia containing asci and unusual long ascospores measuring up to 90 μm. In addition to these morphological characteristics, the maximum parsimony analysis of the ITS region and β-tubulin gene placed the fungus in a distinct clade far from the currently valid Colletotrichum species. Based on the morphological and molecular characterization, Colletotrichum gigasporum sp. nov. was proposed as a new species in the genus Colletotrichum Corda.  相似文献   

7.
Four yeast strains were isolated from rotting wood samples collected from two sites in the Baotianman Nature Reserve and the Laojieling Nature Reserve in China. DNA sequence comparison and other taxonomic characteristics identified the strains as a single novel species of the genus Metschnikowia. The name Metschnikowia henanensis sp. nov. is proposed to accommodate these highly divergent organisms with the type strain BY-97T (= CICC 1982T = CBS 12677T). The novel species produced chlamydospores, but it did not exhibit ascospore formation in sporulation media for 4 weeks. Molecular phylogeny from the D1/D2 domains of the large subunit (LSU) rRNA gene sequences placed this new species in a basal position to the Metschnikowia viticola/Candida kofuensis/Metschnikowia noctiluminum subclade, and an undescribed Candida species namely strains IMB-EMP4 and IMB-EMP5 was a close sister to M. henanensis.  相似文献   

8.
Eleven yeast strains representing two hitherto undescribed species were isolated from different kinds of meat samples in Hungary and one from the sediment of a tropical freshwater river in Southeastern Brazil. The analysis of the sequences of their large subunit rRNA gene D1/D2 domain and the internal transcribed spacer (ITS) regions placed the two new species in the Yarrowia clade. Some of the seven strains representing the first new species can mate and give rise to asci and form ascospores embedded in capsular material, which qualifies it as the third teleomorph species of the Yarrowia clade. The name Yarrowia porcina sp. nov. (type strain: NCAIM Y.02100T = CBS 12935T = NRRL Y-63669T, allotype strain UFMG-RD131A = CBS 12932A) is proposed for this new yeast species, which, based on physiological characters, is indistinguishable from Yarrowia lipolytica and some other species of the genus. Considerable intraspecific variability was detected among the sequences of the large subunit rRNA gene D1/D2 domains of the seven strains. The variability among the D1/D2 sequences exceeded the divergence observed among the ITS sequences and in some cases more than 1 % substitution among the D1/D2 sequences was detected. The conspecificity of these strains was supported by the low (0–3 substitutions) sequence divergence among their ITS sequences, the result of a parsimony network analysis utilizing the concatenated ITS and D1/D2 sequences and also by the fingerprint patterns generated by microsatellite primed PCR. No ascospore formation was observed in the group of the other five strains representing the second new species. These strains shared identical D1/D2 and ITS sequences. Yarrowia bubula f.a., sp. nov. (type strain: NCAIM Y.01998T = CBS 12934T = NRRL Y-63668T) is proposed to accommodate these strains.  相似文献   

9.
Helicomyces geniculatus sp. nov. from decaying wood submerged in a freshwater stream at Juchi Town of Alishan area, Chiayi County, Taiwan, is described and illustrated. It differs from other Helicomyces species in having geniculate conidiophores arising from repent hyphae and also borne on erect setae. Abundant stalked sclerotia were also found in this species. Helicomyces torquatus is described in this paper as a new record for Taiwan, with new observation. The phylogenetic relationship of H. geniculatus, H. torquatus, and related taxa were sought by comparing the sequences of their ITS barcode of the nuc rDNA. A synopsis of the 13 accepted Helicomyces species is given.  相似文献   

10.
Dollar spot is one of the most destructive and economically important fungal diseases of amenity turfgrasses. The causal agent was first described in 1937 as the ascomycete Sclerotinia homoeocarpa. However, the genus-level taxonomic placement of this fungus has been the subject of an ongoing debate for over 75 y. Existing morphological and rDNA sequence evidence indicates that this organism is more appropriately placed in the family Rutstroemiaceae rather than the Sclerotiniaceae. Here we use DNA sequence data from samples of the dollar spot fungus and other members of the Rutstroemiaceae (e.g. Rutstroemia, Lanzia, Lambertella) collected throughout the world to determine the generic identity of the turfgrass dollar spot pathogen. Phylogenetic evidence from three nucleotide sequence markers (CaM, ITS and Mcm7; 1810-bp) confirmed that S. homoeocarpa is not a species of Sclerotinia; nor is it a member of any known genus in the Rutstroemiaceae. These data support the establishment of a new genus, which we describe here as Clarireedia gen. nov. The type species for the genus, Clarireedia homoeocarpa comb. nov., is described to accommodate the dollar spot fungus, and a neotype is designated. Three new species in this clade, Clarireedia bennettii sp. nov., Clarireedia jacksonii sp. nov., and Clarireedia monteithiana sp. nov. that also cause dollar spot disease are described. Clarireedia homoeocarpa and C. bennettii occur primarily on Festuca rubra (C3 grass) hosts and appear to be restricted to the United Kingdom. Clarireedia jacksonii and C. monteithiana occur on a variety of C3 and C4 grass hosts, respectively, and appear to be globally distributed. This resolved taxonomy puts to rest a major controversy amongst plant pathologists and provides a foundation for better understanding the nature and biology of these destructive pathogens.  相似文献   

11.
Recent collections of Ceratozamia from the Sierra Norte of Puebla, Mexico, point to a new undescribed species, which was previously subsumed under the concept of C. mexicana. Identification of this new species resulted from a review of three associated species: C. mexicana, C. tenuis, and C. delucana. Here we describe Ceratozamia totonacorum and provide information regarding its distribution and natural history. The Ceratozamia species from the Sierra Madre Oriental, Mexico, are circumscribed using morphological characters, including a detailed evaluation of reproductive structures such as the apex, position, color and shape of the ovulate strobilus.  相似文献   

12.
Two previously recorded new species of the large-eye seabream genus Gymnocranius (Gymnocranius sp. D and Gymnocranius sp. E) remain undescribed. Here we describe Gymnocranius sp. E as Gymnocranius obesus sp. nov. This new species is morphologically distinct from all other known species under Gymnocranius by the following combination of characters: relatively deep body, with ratio of standard length to body depth 2.2–2.4; protruding large eye, with eye diameter about equal to or slightly larger than inter-orbital width; caudal fin moderately forked; no blue spots or wavy blue lines on cheek and snout in adults; fourth transversal dark bar on flank running from the sixth spine of the dorsal fin to the origin of the anal fin; anal, caudal and dorsal fins drab with yellowish to yellow margins. Gymnocranius obesus sp. nov. is distinct from G. griseus, with which it has been previously confused by a relatively larger head, scales above lateral line without dark basal patch, and a smaller number of front scales on the dorsal side of the head. Gymnocranius obesus sp. nov. is genetically distinct from its closest known relative, Gymnocranius sp. D by 104 diagnostic nucleotide characters, which translates into a 9.6% sequence divergence at the mitochondrial cytochrome b gene. Gymnocranius obesus sp. nov. reaches a length of at least 295 mm. Its distribution, from the Ryukyu Islands to Bali, including Taiwan and the Flores Sea, mostly coincides with the western half of the Coral Triangle.  相似文献   

13.
Thirteen undescribed strains of ballistoconidium-forming yeasts, isolated from leaves collected in the suburbs and along the southeast seacoast of Bangkok, Thailand, were divided into four different groups in the genusSporobolomyces on the basis of morphological, physiological, biochemical, and chemotaxonomical characteristics, and analyses of the sequences of 18S rDNA and internal transcribed spacer regions. DNA-DNA reassociation experiments with related species revealed that these four groups were four new distinct species.Sporobolomyces nylandii sp. nov.,S. poonsookiae sp. nov.,S. blumeae sp. nov. andS. vermiculatus sp. nov. are proposed for these strains.  相似文献   

14.
The section Brunnei was extensively studied based on material from North Europe. To stabilise the nomenclature we studied the relevant types of taxa included in this section. Phylogenetic relationships and species limits were investigated using rDNA ITS sequences and the results were compared with the morphological data. We recognised 11 species: Cortinarius brunneus, C. clarobrunneus comb. nov., C. coleoptera, C. ectypus, C. gentilis, C. glandicolor (neotypified), C. pseudorubricosus, and four species described as new C. caesiobrunneus, C. albogaudis, C. carabus, and C. cicindela. They are described here and their taxonomy, ecology, distribution, and relationships are discussed. In addition, a key to species of the section Brunnei is provided. A total of 77 new sequences of 11 species are published including nine type sequences. Also the taxonomic assignments of sequences in the public databases belonging to the section Brunnei are revised.  相似文献   

15.
Three freshwater scuticociliates, Apouronema harbinensis gen. nov. spec. nov., Cyclidium vorax spec. nov., and C. glaucoma Müller, 1773, collected from rivers in Hulan District, Harbin, northeastern China, were investigated using morphological and phylogenetic criteria. Apouronema gen. nov., assigned to the family Uronematidae, is mainly distinguished from the other genera of the family by its paroral membrane extending anteriorly to the middle of membranelle 1. Apouronema harbinensis spec. nov. is defined by body size in vivo about 45–55 × 20–25 μm, buccal field about 70–80% of cell length; 12 or 13 somatic kineties; membranelle 1 having two rows, with 16–18 basal bodies in each kinety; membranelle 2 and membranelle 3 both having two rows each; scutica X-shaped with five pairs of basal bodies. Cyclidium vorax spec. nov. is characterized by the following features: body size 35–40 × 18–20 μm in vivo; 9 or 10 somatic kineties; membranelle 1 having two longitudinal rows, much shorter than M2; M2 triangle-shaped. The phylogenetic analyses show that: (1) Apouronema clustered in the Uronematidae clade, and grouped with genera Uronemita and Uronema; (2) Cyclidium vorax spec. nov. grouped with C. glaucoma and C. sinicum, which supports the assignment of the new species to the genus Cyclidium; (3) Cyclidium remains non-monophyletic with the addition of the new sequence.  相似文献   

16.
The Japanese golden chanterelle commonly identified as Cantharellus cibarius was sampled in a broad range of forest vegetation. A total of 90 fresh and 11 herbarium specimens were examined microscopically, subjected to sequencing analysis of their nuclear ribosomal RNA (rDNA) and tef-1 genes, and their characteristics were compared with those of European C. cibarius. Based on morphological and ecological characteristics, basidioma samples from Japan were divided into four species. While specimens of Cantharellus sp. 4 from Hokkaido Island were included in the European C. cibarius clade phylogenetically, the other three species formed three unique clades. Among these, Cantharellus anzutake sp. nov. is sister to the clade of C. cibarius and was widely sampled from the northern limit of Honshu Island to the southern limit of Kumejima Island in Ryukyu Islands. Although C. anzutake was morphologically similar to C. cibarius, the two species were phylogenetically distinct. Other morphologically similar but genetically distinct chanterelle species from India exhibited macroscopic and microscopic differences compared with C. anzutake.  相似文献   

17.
18.
The Hippasterinae is a subfamily within the Goniasteridae, consisting of five genera and 26 species, which occur in cold‐water settings ranging from subtidal to abyssal depths. All known genera were included in a cladistic analysis resulting in two most parsimonious trees, supporting the Hippasterinae as monophyletic. Our review supports Sthenaster emmae gen. et sp. nov. as a new genus and species from the tropical Atlantic and two new Evoplosoma species, Evoplosoma claguei sp. nov. and Evoplosoma voratus sp. nov. from seamounts in the North Pacific. Hippasteria caribaea is reassigned to the genus Gilbertaster, which previously contained a single Pacific species. Our analysis supports Evoplosoma as a derived deep water lineage relative to its continental‐shelf, shallow water sister taxa. The genus Hippasteria contains approximately 15 widely distributed, but similar‐looking species, which occur in the northern and southern hemispheres. Except for Gilbertaster, at least one species in each genus has been observed or is inferred to prey on deep‐sea corals, suggesting that this lineage is important to the conservation of deep‐sea coral habitats. The Hippasterinae shares several morphological similarities with Circeaster and Calliaster, suggesting that they may be related. © 2010 The Linnean Society of London, Zoological Journal of the Linnean Society, 2010, 160 , 266–301.  相似文献   

19.
Alpha taxonomy, and specifically the delimitation of species, is becoming increasingly objective and integrative. The use of coalescent-based methods applied to genetic data is providing new tools for the discovery and delimitation of species. Here, we use an integrative approach via a combination of discovery-based multivariate morphological analyses to detect potential new species. These potential species are then used as a priori species in hypothesis-driven validation analyses with genetic data. This research focuses on the harvestmen genus Sclerobunus found throughout the mountainous regions of western North America. Based on our analyses, we conduct a revision of Sclerobunus resulting in synonymy of Cyptobunus with Sclerobunus including transfer of S. cavicolens comb. nov. and elevation of both subspecies of S. ungulatus: S. ungulatus comb. nov. and S. madhousensis comb. nov., stat. nov. The three subspecies of S. robustus are elevated, S. robustus, S. glorietus stat. nov., and S. idahoensis stat. nov. Additionally, five new species of Sclerobunus are described from New Mexico and Colorado, including S. jemez sp. nov., S. klomax sp. nov., S. skywalkeri sp. nov., S. speoventus sp. nov., and S. steinmanni sp. nov. Several of the newly described species are single-cave endemics, and our findings suggest that further exploration of western North American cave habitats will likely yield additional new species.  相似文献   

20.
A new pearleye species of the alepisauroid family Scopelarchidae, Scopelarchoides neamticus sp. nov., is described herein based on two specimens from the Oligocene Lower Dysodilic Shales Formation, cropping out in the Pietricica Mountain, Romanian Eastern Carpathians. The new species described herein exhibits a unique combination of features (including head length about 25% of SL; coracoid remarkably expanded; both preorbital and postorbital lengths larger than orbit diameter; 50 or 51 vertebrae; dorsal fin with nine or ten rays; anal-fin with 28 rays; length of anal fin base about 30% of SL; preanal distance almost 60% of SL; pelvic fin insertion located just under the second dorsal fin ray; pectoral fins only slightly longer than pelvic fins; caudal fin with 19 principal rays plus 14 upper and 13 lower procurrent rays) that justifies its recognition as a new species of the genus Scopelarchoides. Both morphological and meristic features suggest a certain degree of similarity between S. neamticus sp. nov. and the extant species Scopelarchoides signifer. The fossils of the new Oligocene species described herein represent the oldest known skeletal record of Scopelarchidae.  相似文献   

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