两种滇产鼠尾草复方注射液对大鼠在体心肌缺血再灌注损伤的保护作用

与复方丹参注射液（丹参＋三七）比较,探讨两种滇产鼠尾草复方注射液。即复方滇丹参（滇丹参＋三七）和复方甘西鼠尾（甘西鼠尾＋三七）对大鼠在体心肌缺血再灌注损伤的保护作用。采用在体缺血再灌注模型,缺血30min复灌60min。实验过程中用II导联记录心电图ST段和心率变化,实验后测定心室梗塞面积。结果：这两种复方鼠尾草注射液能显著降低在体心肌缺血和再灌注过程中ST段抬高水平。轻度加快缺血再灌注过程心率,相似于复方丹参;这两种鼠尾草复方注射液均能明显减少心室梗塞面积。这两种滇产鼠尾草复方注射液均有较好的抗心肌缺血再灌注损伤作用,与复方丹参注射液相似。     

复方丹参注射液联合乌司他汀治疗急性胰腺炎的效果

目的:观察急性胰腺炎患者采用复方丹参注射液联合乌司他汀治疗的效果。方法:选取2013年9月-2014年9月于本院治疗的98例急性胰腺炎患者,将其随机分为2组,观察组49例,对照组49例,对照组给予乌司他汀,观察组予以复方丹参注射液联合乌司他汀治疗,观察对比两组临床疗效。结果:观察组总有效率为93.9%,对照组为77.6%,两组疗效对比观察组明显优于对照组(P0.05)。结论:采用复方丹参注射液联合乌司他汀治疗胰腺炎疗效显著,值得临床上推广应用。     

丹红注射液治疗心脑血管疾病的药理作用研究进展

丹红注射液是在中医脑心同治理论指导下,采用活血化瘀中药丹参、红花为主要原料的一种制剂。目前,丹红注射液广泛应用于冠心病心绞痛、脑梗死、慢性肺源性心脏病、血管性痴呆等疾病的治疗,药理研究表明其具有抗炎、抗氧化、抗凝血、降血脂、促血管新生等多方面作用,可通过多途径、多靶点对疾病进行干预。笔者通过搜索中国知网(CNKI)和PUBMED中收录的文献,主要对近5年来丹红注射液治疗心脑血管疾病的药理作用进行综述,为丹红注射液的临床应用提供参考。     

复方丹参注射液联合阿奇霉素治疗小儿喘息性支气管炎的效果及对炎性因子的影响

目的:探讨复方丹参注射液联合阿奇霉素治疗小儿喘息性支气管炎的效果及对炎性因子的影响。方法:选取2015年6月～2018年6月我院收治的喘息性支气管炎患儿300例,采用随机数字表法将患儿分为两组,每组各150例。对照组在常规治疗的基础上给予阿奇霉素注射液治疗,观察组在对照组的基础上联合应用复方丹参注射液治疗。比较两组的临床治疗效果,临床症状缓解时间及住院时间,治疗前后两组血清白介素(Interleukin, IL)-6、IL-8和肿瘤坏死因子-α(Tumor necrosis factor-α, TNF-α)水平的变化情况及不良反应发生情况和复发率。结果:治疗后,观察组治疗总有效率显著高于对照组(93.33%VS.85.33%, P0.05);观察组喘息缓解时间、咳嗽缓解时间、哮鸣音消失时间、体温恢复时间及住院时间均显著短于对照组(P0.05);两组治疗后血清IL-6、IL-8和TNF-α水平均较治疗前显著下降,且观察组更低(P0.05);两组不良反应发生率比较差异无统计学意义(P0.05),观察组的复发率显著低于对照组(P0.05)。结论:复方丹参注射液联合阿奇霉素可快速缓解喘息性支气管炎患儿的临床症状、体征并缩短住院时间,提高临床治疗效果,且复发率低,安全性较高,这可能与其可显著降低患儿血清IL-6、IL-8和TNF-α水平有关。     

分析金纳多注射液对治疗脑梗塞患者的临床疗效

目的:分析金纳多注射液对治疗脑梗塞患者的临床疗效。方法:选取本院于2013年2月~2014年2月收治的42例缺血性脑卒中患者,将其分为治疗组及对照组。治疗组21例患者采用金纳多注射液进行治疗,对照组21例患者采用复方丹参注射液进行治疗。对比分析两组患者的临床治疗效果。结果:经过治疗后,治疗组患者基本痊愈12例,显著进步8例无变化1例,恶化0例,总有效率为95.23%;对照组患者基本痊愈7例,显著进步8例,无变化4例,恶化2例,总有效率为71.42%。结论:相对于复方丹参注射液而言,金纳多注射液对缺血性脑卒中患者的治疗具有显著的效果。     

小鼠体内血栓形成动物模型的建立及在抗血栓药物筛选中的 …

建立一种无创性小鼠体内血栓动物模型并于多种抗栓药物的药效观察。方法：用角叉菜胶建立了小鼠体内血栓动物模型。利用此模型观察潘生丁片,抗栓胶囊、复方丹参注射液、央求地血栓和的影响。结果及结论：用角叉菜胶建立的小鼠体内血栓动物模型,无创伤性、操作简易方便。并可应用于多种抗血栓药物的药效观察,剂量的选择,药物的筛选。     

丹参素注射液对急性心肌梗死大鼠的心室重构、心室功能及肢体导联与胸导联心电图参数的影响

摘要 目的：探讨丹参素注射液对急性心肌梗死大鼠的心室重构、心室功能及肢体导联与胸导联心电图参数的影响。方法：选择SD大鼠40只,将其鼠随机模型组、假手术组、硝酸甘油组、丹参注射液组。假手术组大鼠给予只在冠状动脉处穿针,不进行结扎,其余步骤同其余3组,其余3组均进行动物模型构建。假手术组、模型组大鼠均腹腔注射氯化钠注射液,硝酸甘油组腹腔注射硝酸甘油,丹参注射液组腹腔注射丹参注射液。对比4组大鼠的肢体导联与胸导联心电图参数,对比4组大鼠的血液流变学指标、左心室功能及左心室重构。结果：模型组的Ⅰ、Ⅱ、Ⅲ、aVL、aVF、V1、V2、V5、血浆粘度、纤维蛋白原、红细胞聚集指数、舒张末期室间隔厚度、左室舒张末期内径、左室收缩末期内径、左室舒张末期容积、左室收缩末期容积明显较假手术组、硝酸甘油组、丹参注射液组高,硝酸甘油、丹参注射液组以上指标明显较假手术组高,模型组的的左室舒张末期厚度、左室射血分数、左室短轴缩短率明显较假手术组、硝酸甘油组、丹参注射液组低,硝酸甘油、丹参注射液组的左室舒张末期厚度、左室射血分数、左室短轴缩短率明显较假手术组低。模型组的左心室重量指数、左心室截面直径明显较假手术组、硝酸甘油组、丹参注射液组高,硝酸甘油、丹参注射液组的左心室重量指数、左心室截面直径、梗死面积明显较假手术组高（P<0.05）,硝酸甘油组与丹参注射液组以上指标对比无差异（P>0.05）。结论：丹参素注射液可改善急性心肌梗死大鼠的心室重构、左心室功能及肢体导联与胸导联心电图参数,可能与其可降低大鼠的血液流变学指标水平有关。     

不同剂量雷公藤多苷联合复方丹参对过敏性紫癜性肾炎患者凝血功能及内皮细胞功能的影响

目的:考察不同剂量雷公藤多苷联合复方丹参对过敏性紫癜性肾炎(HSPN)患者凝血功能及内皮细胞功能的影响,并评价疗效。方法:选取我院2016年9月至2018年3月收治的72例HSPN患者,按照随机数字表法分成对照组(静脉滴注复方丹参注射液治疗)、低剂量组(1.0 mg/kg雷公藤多苷联合复方丹参注射液治疗)和高剂量组(1.5 mg/kg雷公藤多苷联合复方丹参注射液治疗),每组各24例,三组均治疗12周。分别于治疗前后检测三组患者凝血酶原时间(PT)、凝血酶时间(TT)、纤维蛋白原(FIB)水平、活化部分凝血活酶时间(APPT)、血小板计数(PLT)、内皮细胞生长因子(VEGF)、内皮素-1(ET-1)水平及血流介导的血管扩张功能(FMD)。评价三组疗效,并观察治疗过程中出现的不良反应。结果:治疗后低剂量组、高剂量组TT、APPT较治疗前和对照组明显升高,FIB和PLT明显降低,且高剂量组较低剂量组变化更明显(P0.05),治疗后高剂量组PT高于治疗前、对照组和低剂量组(P0.05)。治疗后低剂量组、高剂量组VEGF及ET-1水平较治疗前降低,FMD值较治疗前升高,且高剂量组VEGF及ET-1水平低于对照组和低剂量组,FMD值高于对照组和低剂量组(P0.05)。高剂量组的有效率为95.83%(23/24),高于低剂量组的75.00%(18/24)和对照组的42.67%(10/24)。三组不良反应发生率无明显差异(P0.05)。结论:高剂量雷公藤多苷与复方丹参注射液合用较低剂量的合用可明显改善HSPN患者的凝血功能并恢复内皮细胞功能,疗效明显,安全性高。     

侧脑室内注射复方丹参注射液对大鼠肺动脉压的影响

复方丹参注射液 (ｓａｌｖｉａｌｍｉｌｔｉｏｎｒｒｈｉｊａｅ,ＳＭ) ,具有扩张血管 ,改善微循环 ,护肝抗凝等作用 ,临床上主要用于治疗心绞痛 ,心肌梗塞等疾病 ,关于ＳＭ降低肺动脉压的作用早有报道 ,但其对中枢作用尚未见报道 ,本研究主要是从侧脑室注射微量ＳＭ以观察其对肺动脉压的作用。1　材料与方法(1)材料　实验用药均用新鲜生理盐水配制 ,丹参注射液为十堰康迪制药厂生产 (ＳＭ ,ｐＨ 6 .8) ,心得安 (Ｐｒｏｐｒａｎｏｌｏｌ,ｐＨ6 .8) ,酚妥拉明 (Ｐｈｅｎｔｏｌａｍｉｎｅ ,ｐＨ ,6 .8) ,阿托品 (Ａｔｒｉｏｐｉｎｇ ,ｐＨ6 .8)…     

丹参对冻融入胎儿卵巢组织异种移植早期血管生成的影响

目的：研究冻融人胎儿卵巢组织移植早期血管生成过程中微血管形态和密度的改变以及血管生成相关基因mRNA的表达;探讨丹参注射液对移植物血管生成的影响。方法：冻融胎儿卵巢组织异种移植至裸鼠肾被膜下,按给药不同分为对照组（生理盐水）和丹参组（丹参注射液每只0．09g／d）,分别于移植后48h、7d和28d回收移植物。结果：移植后两组卵巢组织微血管密度均明显增多;对照组移植后7d血管密度达峰,丹参组血管密度在移植后48h即已显著上升,此后两个时间段保持相对平稳。Angiopoietin-2mRNA表达在移植后48h两组均显著升高,丹参组上升幅度大于对照组（P〈0．05）。结论：冻融人胎儿卵巢组织新血管生成开始于异种移植后48h内,移植后7d组织内微血管密度达峰。丹参在移植早期应用可以促进移植后血管生成,其机制可能与它增加了血管生成相关因子Ang-2mRNA的表达有关。     

丹参注射液与丹参多酚酸盐注射液对不稳定型心绞痛患者冠状动脉微循环的影响

目的:比较丹参注射液与丹参多酚酸盐注射液对不稳定型心绞痛(UA)患者冠状动脉微循环的影响。方法:将2014年5月～2017年5月105例UA患者随机分为丹参组(n=50)与丹参多酚酸盐组(n=55),前者在PCI术前静脉滴注丹参注射液20 mL,1次/d,连续3 d;后者静脉滴注丹参多酚酸盐注射液200 mg,1次/d,连续3 d。分别在PCI术前及术后即刻检测冠状动脉血流储备(CFR)、冠状动脉微循环阻力系数(IMR)及TIMI血流分级。结果:两组术后CFR、IMR及TIMI血流分级均较术前明显改善(P0.05),丹参多酚酸盐组IMR明显小于丹参组(P0.05),CFR、TIMI血流分级与丹参组比较无统计学意义(P0.05)。结论:丹参注射液与丹参多酚酸盐注射液均能显著改善UA患者的冠状动脉微循环,丹参多酚酸盐注射液一定程度上优于丹参注射液。     

Protective effects of Danshensu from the aqueous extract of Salvia miltiorrhiza (Danshen) against homocysteine-induced endothelial dysfunction

Homocysteine (Hcy) is a by-product of methionine metabolism. An imbalance of Hcy in the body may lead to hyperhomocysteinemia, a condition with elevated Hcy concentration in blood that may be one of the risk factors responsible for the development of several vascular diseases (thromboembolism, atherosclerosis, stroke, vascular diseases and dementia). Radix Salvia miltiorrhiza (Danshen), a well-known Chinese medicinal herb that can activate and improve blood microcirculation, is noticeable for its beneficial effect in treating cardiovascular diseases. The present study is to demonstrate the protective effect of Danshen extract against the homocysteine-induced adverse effect on human umbilical vein endothelial cell (HUVEC). Homocysteine (5 mM) not only decreased the cell viability but also caused the disruption of capillary-like structure formation in vitro. The protective effect of Danshen aqueous extract and its active compounds on endothelial cell function were demonstrated through an in vitro tube formation assay, which mimics the new blood vessel formation. To identify the active components in the aqueous extract of Danshen, the content was characterized by instrumental analysis using high performance liquid chromatography with diode array detector (DAD) and electrospray tandem mass spectrometry (ESI-MS/MS). Interestingly, Danshen extract and its pure compounds showed different effectiveness in protecting HUVEC against Hcy-induced injury according to the following descending order: Danshen aqueous extract, 3-(3,4-dihydroxy-phenyl)-2-hydroxy-propionic acid (Danshensu), protocatechuic acid, catechin and protocatechualdehyde. We believed that such findings might provide evidence in understanding the beneficial effects of Danshen on the cardiovascular system.     

Development of the fingerprints for the quality of the roots of Salvia miltiorrhiza and its related preparations by HPLC-DAD and LC-MS(n)

High-performance liquid chromatographic (HPLC) fingerprints were developed for identification of both lipophilic and hydrophilic components of the roots of Salvia miltiorrhiza and four related preparations. These samples were separated with an Agilent Zorbax Extend C(18) reserved-phase column (5 microm, 250 mm x 4.6 mm) by linear gradient elution using water-phosphoric acid (100:0.026, v/v) and acetonitrile as mobile phase. The flow rate was 0.8 ml/min and the detector wavelength was set at 280 nm. Mean chromatograms and correlation coefficients of samples were calculated by the software "Similarity Evaluation System for Chromatographic Fingerprint of TCM". The correlation coefficients of Danshen and Fufang Danshen tablets (FDT) samples were in the range of 0.352-0.993 and 0.768-0.987, respectively. The correlation coefficients of Compound Danshen dripping pills (CDDP), Danshen injection (DSI) and Xiangdan injection (XDI) samples were higher than 0.928, 0.850 and 0.960, respectively. It was the first time to identify 34 peaks by comparing with standard compounds and using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS(n)) technique. All results indicated that the developed fingerprint assay could be readily utilized as a quality control method for S. miltiorrhiza and its related preparations.     

康复训练、针灸联合丹参川芎嗪穴位注射对脑梗死患者肩手综合征的影响

目的:探讨康复训练、针灸联合丹参川穹嗪穴位注射对脑梗死患者肩手综合征的影响。方法:选取2014年3月至2016年3月于我院诊治的脑梗死后肩手综合征(SHS)患者82例,随机平均分为A、B组各41例。A组患者予综合药物治疗、针灸及康复训练,B组在A组基础上给予丹参川穹嗪穴位注射治疗。分析比较两组患者治疗前后的上肢运动功能评分(FMA)、数字疼痛评分(NRS)、肩关节肿胀程度、肩关节活动度(ROM)、神经功能缺损评分(CNFDS)、日常生活活动能力(MBI)和生活质量评分(WHOQOL)。结果:治疗后,两组患者的NRS评分、肩关节肿胀程度、上肢CNFDS评分均较治疗前显著降低,上肢FMA评分、MBI、WHOQOL评分、各个范围ROM均明显增加,且B组以上指标较A组改善更明显(P0.05)。结论:康复训练、针灸联合丹参川穹嗪穴位注射治疗脑梗死后肩手综合征疗效显著优于药物、针灸治疗及康复训练。     

藏红花对肝纤维化大鼠肝组织TGF-β1 表达的影响

目的:通过观察藏红花对肝纤维化大鼠肝组织TGF-β1表达的影响,探讨藏红花预防肝纤维化作用的机制。方法:清洁级SD雄性大鼠60只随机分为正常组、模型组、藏红花组、丹参组,每组十五只。应用30%四氯化碳橄榄油溶液3ml/kg.腹腔注射制备肝纤维化大鼠模型。治疗8周后,通过HE和Masson染色观察肝纤维化的形成、免疫组化检测肝组织中TGF-β1蛋白的表达。结果:臧红花组、丹参组与模型组相比肝纤维化程度均轻于模型组(P<0.01),而藏红花组与丹参组相比肝纤维化程度无显著性差异(P>0.05),臧红花组、丹参组与模型组相比TGF-β1蛋白的表达均明显减少,而藏红花组与丹参组相比TGF-β1蛋白的表达没有显著性差异(P>0.05)。结论:藏红花能有效减轻肝纤维化大鼠的肝脏损伤及纤维化程度,其机制可能与抑制肝内TGF-β1的表达,抑制HSC的激活以及阻断HSC与TGF-β1之间的恶性循环有关。     

高速逆流色谱法分离制备丹酚酸B

采用高速逆流色谱法分离纯化丹参水溶性成分丹酚酸类物质,制备丹酚酸B化学对照品。分离采用的溶剂系统为正己烷-乙酸乙酯-水-甲醇(1.5:5:5:1.5),上相做固定相,下相做流动相,流速为1.7 mL/min,仪器转速850 rpm,进样量80 mg,纯度用HPLC方法测定。结果表明:一次分离可制备63.4 mg丹酚酸B,其纯度为98.6%。该方法操作简单,可作为高纯度丹酚酸B化学对照品的制备分离方法。     

舒血宁注射液联合丹参滴丸对急性冠脉综合征患者血脂和心功能的影响

摘要 目的：探讨舒血宁注射液联合丹参滴丸对急性冠脉综合征（ACS）患者血脂和心功能的影响。方法：选取2015年2月~2019年12月期间我院收治的ACS患者90例,按照随机数字表法将上述患者分为对照组（n=45,常规基础治疗）和研究组（n=45,增加舒血宁注射液联合丹参滴丸治疗）,比较两组患者疗效、血脂、心功能及不良反应。结果：研究组治疗2个疗程后的临床总有效率为91.11%（41/45）,高于对照组的73.33%（33/45）（P<0.05）。两组治疗2个疗程后总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、甘油三酯（TG）均较治疗前降低,且研究组低于对照组（P<0.05）;高密度脂蛋白胆固醇（HDL-C）较治疗前升高,且研究组高于对照组（P<0.05）。两组治疗2个疗程后左心室射血分数（LVEF）、心脏指数（CI）、心排血量（CO）均较治疗前升高,且研究组高于对照组（P<0.05）;左室舒张末期内径（LVEDD）较治疗前降低,且研究组低于对照组（P<0.05）。两组治疗期间不良反应发生率比较差异无统计学意义（P>0.05）。结论：舒血宁注射液联合丹参滴丸治疗ACS,疗效显著,可有效改善患者血脂及心功能,且不增加不良反应发生率,安全可靠。     

In vitro inhibitory effects of ethanol extract of Danshen (Salvia miltiorrhiza) and its components on the catalytic activity of soluble epoxide hydrolase

Background: Soluble epoxide hydrolase (sEH) has been demonstrated to be a key enzyme involved in the pathologic development of several cardiovascular diseases and inflammation, and inhibition of sEH is therefore very helpful or crucial for the treatment of ischemia-reperfusion injury, cardiac hypertrophy, hypertension and inflammation. Danshen, the dried root of Salvia miltiorrhiza (Fam. Labiatae), has been used for the treatment of cardiovascular and cerebrovascular diseases in China and other countries for hundreds of years. Recent studies indicated that Danshen and its preparations also have potential for the management of inflammation. However, little information is available about the possibility of Danshen and its components on sEH inhibition.Purpose and methods: Danshen extracts and its constituents were tested for sEH inhibition using its physiological substrate, 8,9-EET, based on a LC–MS/MS assay in this study.Results: Among the tested 15 compounds, tanshinone IIA and cryptotanshinone were found to be the potent (K_i = 0.87 μM) and medium (K_i = 6.7 μM) mixed-type inhibitors of sEH, respectively. Salvianolic acid C (K_i = 8.6 μM) was proved to be a moderate noncompetitive sEH inhibitor. In consistent with the inhibition results of the pure compounds, the 75% ethanol extract of Danshen (EE, IC₅₀ = 86.5 μg/ml) which contained more tanshinone IIA and cryptotanshinone exhibited more potent inhibition on sEH than the water extract (WE, IC₅₀ > 200 μg/ml) or 1 M NaHCO₃ (BE, IC₅₀ > 200 μg/ml) extract.Conclusion: These data indicated that using the ethanol fraction of Danshen and increasing the amounts of tanshinone IIA, cryptotanshinone and salvianolic acid C, especially the contents of tanshinone IIA in Danshen extract or preparations to enhance the inhibitory effects on sEH might be efficient ways to improve its cardiovascular protective and anti-inflammatory effects, and that herbal medicines could be an untapped reservoir for sEH-inhibition agents and developing sEH inhibitors from the cardiovascular protective and anti-inflammatory herbs is a promising approach.     

Adeno-associated virus-mediated gene transfer to the neonatal brain

For many metabolic diseases, early treatment is necessary to prevent irreversible developmental damage. This is particularly true for childhood diseases that affect the central nervous system (CNS). The development of effective techniques for gene transfer to the neonatal brain would provide a new set of therapeutic options for many of these disorders. Vectors based on adeno-associated virus (AAV) have shown promise as agents for neonatal CNS transduction. In preclinical animal models, a single treatment with AAV vectors at birth has been shown to produce persistent CNS expression of transduced genes into adulthood. Transduction of the neonatal brain has been accomplished by a variety of methods, including direct intraparenchymal injection, intraventricular infusion, and intravenous administration. Of these methods, intraparenchymal injection provides the highest levels of localized activity, while intraventricular infusion results in a more widespread distribution of activity when performed in the neonate. Here we describe a method for direct, intraparenchymal injection of AAV into the neonatal brain. This technique provides a method for investigators to evaluate the effects of in vivo expression of exogenous genes on the process of early brain development.     

Cerebral vasodilator properties of Danshen and Gegen: A study of their combined efficacy and mechanisms of actions

Danshen and Gegen are two commonly used Chinese herbal medicines for treatment of cardiovascular diseases. The aim of the present study was to elucidate the combination effects of these two herbs on cerebral vascular tone and their underlying mechanisms of actions. Basilar artery rings were obtained from rats and precontracted with U46619. Cumulative administrations of aqueous extracts of Danshen, Gegen, or the two herbs combined (DG; ratio 7:3) produced concentration-dependent relaxation of the artery rings. Statistical analysis on these findings produced a combination index (CI) of 1.041 at ED₅₀, which indicates the two herbs produced additive vasodilator effects when used as a combined decoction. Removal of the endothelium had no effect on the vasodilator properties of Danshen, Gegen, and DG. However, their maximum effects (Imax) were significantly blunted by a K_ATP channel inhibitor glibenclamide, a non-selective K⁺ channel inhibitor tetraethylammonium (TEA), and by a combination of K⁺ channel inhibitors (glibenclamide + TEA + iberiotoxin + 4-aminopyridine + barium chloride). In addition, Danshen, Gegen, and DG produced augmentation of K_ATP currents and inhibited Ca²⁺ influx in vascular smooth muscle cells isolated from rat basilar arteries. Furthermore, these agents inhibited CaCl₂-induced contraction in the artery rings. In conclusion, the present study showed that Danshen and Gegen produced additive vasodilator effects on rat cerebral basilar arteries. These effects were independent of endothelium-derived relaxant factors (EDRF), but required the opening of K_ATP channels and inhibition of Ca²⁺ influx in the vascular smooth muscle cells. It is suspected that the cerebral vasodilator effects of Danshen and Gegen produced either on their own or in combination, can help patients with obstructive cerebrovascular diseases.