龙胆苦苷镇痛抗炎药理作用研究

本文采用小鼠醋酸扭体法和热板法观察龙胆苦苷(gentiopicroside)的镇痛药理作用,采用二甲苯致小鼠耳廓肿胀、蛋清致大鼠足跖肿胀急性炎症模型及大鼠肉芽肿慢性炎症模型,考察龙胆苦苷的抗炎药理作用.龙胆苦苷经皮下注射给药,在小鼠醋酸扭体和热板法实验中,减少了扭体次数,提高了小鼠痛域阈值,呈良好的量效关系.明显减轻了二甲苯致小鼠耳廓肿胀,降低蛋清致大鼠足趾肿胀,抑制了大鼠肉芽肿.结果表明龙胆苦苷对热和化学刺激引起的疼痛反应有明显的镇痛作用,对急、慢性炎症反应均有一定的抑制作用.     

姜延胶囊抗炎镇痛作用实验研究

目的:观察姜延胶囊的抗炎镇痛作用.方法:采用醋酸致小鼠腹腔毛细管通透法、二甲苯致小鼠耳廓肿胀法、滤纸性肉芽肿法及小鼠热板法、扭体法镇痛实验等动物模型,考察姜延胶囊的抗炎镇痛效果.结果:姜延胶囊能明显减轻小鼠腹腔毛细血管通透性,对小鼠耳肿胀及肉芽肿均有抑制作用;并能减少热板及醋酸所致的小鼠疼痛反应,提高小鼠痛阈值.结论:姜延胶囊具有良好的抗炎镇痛作用,该实验为其临床应用提供了药理学依据.     

乌头总生物碱贴片抗炎镇痛药效学研究

本文采用蛋清所致大鼠足跖肿胀和小鼠二甲苯所致耳廓肿胀模型研究乌头总生物碱贴片的抗炎作用及化学刺激法和热板法研究乌头总生物碱贴片对小鼠的镇痛作用进行实验.镇痛抗炎实验表明,乌头总生物碱贴片能提高小鼠热板法所致疼痛的痛阈值并减少醋酸所致小鼠扭体反应次数;对二甲苯所致小鼠耳肿胀有明显的抑制作用,能明显减轻蛋清所致的大鼠足跖肿胀.结果表明乌头总生物碱贴片具有明显的抗炎及镇痛作用.     

大叶胡枝子根皮抗炎镇痛活性部位及其机制北大核心CSCD

筛选大叶胡枝子根皮抗炎镇痛作用的活性部位,并探讨其作用机制。采用二甲苯致小鼠耳肿胀、蛋清致大鼠足跖肿胀以及大鼠棉球肉芽肿炎症模型,考察大叶胡枝子根皮抗炎作用的活性部位,采用热板和醋酸扭体疼痛模型,考察大叶胡枝子根皮镇痛作用的活性部位,并检测活性部位对蛋清致炎模型大鼠血清的TNF-α、IL-1β和足跖炎性组织的PGE_2含量以及醋酸致痛模型小鼠血清的NO、MDA和PGE_2含量,以初步探究其抗炎镇痛作用机制。结果表明:大叶胡枝子根皮石油醚萃取部位明显抑制二甲苯所致小鼠耳肿胀和蛋清所致大鼠足跖肿胀以及棉球肉芽肿,明显延长热板所致小鼠痛阈值和明显减少醋酸所致小鼠扭体反应次数;明显减少蛋清致炎大鼠血清TNF-α、IL-1β及足跖炎性组织PGE_2含量,明显降低醋酸致痛小鼠血清NO、MDA和PGE_2含量。结果显示,大叶胡枝子根皮抗炎镇痛作用的活性部位主要为石油醚部位,其抗炎机制可能与减少炎症时TNF-α、IL-1β和PCE_2的产生有关,其镇痛作用机制可能与降低疼痛时NO、MDA和PGE_2的含量有关。     

大叶胡枝子根皮抗炎镇痛活性部位及其机制

筛选大叶胡枝子根皮抗炎镇痛作用的活性部位,并探讨其作用机制。采用二甲苯致小鼠耳肿胀、蛋清致大鼠足跖肿胀以及大鼠棉球肉芽肿炎症模型,考察大叶胡枝子根皮抗炎作用的活性部位,采用热板和醋酸扭体疼痛模型,考察大叶胡枝子根皮镇痛作用的活性部位,并检测活性部位对蛋清致炎模型大鼠血清的TNF-α、IL-1β和足跖炎性组织的PGE_2含量以及醋酸致痛模型小鼠血清的NO、MDA和PGE_2含量,以初步探究其抗炎镇痛作用机制。结果表明:大叶胡枝子根皮石油醚萃取部位明显抑制二甲苯所致小鼠耳肿胀和蛋清所致大鼠足跖肿胀以及棉球肉芽肿,明显延长热板所致小鼠痛阈值和明显减少醋酸所致小鼠扭体反应次数;明显减少蛋清致炎大鼠血清TNF-α、IL-1β及足跖炎性组织PGE_2含量,明显降低醋酸致痛小鼠血清NO、MDA和PGE_2含量。结果显示,大叶胡枝子根皮抗炎镇痛作用的活性部位主要为石油醚部位,其抗炎机制可能与减少炎症时TNF-α、IL-1β和PCE_2的产生有关,其镇痛作用机制可能与降低疼痛时NO、MDA和PGE_2的含量有关。     

藏药赛北紫堇总生物碱镇痛药效学研究

本文采用小鼠热板法和醋酸扭体法观察藏药赛北紫堇总生物碱的镇痛作用;藏药赛北紫堇总生物碱灌胃给药,在小鼠热板法和扭体法实验中能提高小鼠热刺激引起的痛阈值(P＜0.05),显著减少醋酸所致的小鼠扭体反应(P＜0.05).结果表明藏药赛北紫堇总生物碱具有明显的镇痛作用.     

活络效灵丹抗炎镇痛作用的实验研究

目的：研究活络效灵丹的抗炎、镇痛、消肿等药理作用,为该药的临床研究提供基础。方法：用二甲苯致小鼠耳肿胀法和角又莱胶致大鼠足肿胀法研究抗炎作用,用热板法和扭体法研究镇痛作用。结果：活络效灵丹能较好地抑制二甲苯引起的小鼠耳廓炎性肿胀和大鼠甲醛致足跖肿胀,能显著提高热板试验小鼠的痛阈,有效抑制冰醋酸引起的小鼠扭体反应次数。结论：活络效灵丹具有良好的抗炎、镇痛、消肿等作用。     

小茴香挥发油的抗炎镇痛作用

目的：研究小茴香挥发油的抗炎、镇痛作用,为指导临床合理用药提供科学依据。方法：应用二甲苯致小鼠耳廓肿胀、蛋清致大鼠足肿胀2种动物模型进行抗炎药效学实验;采用醋酸致小鼠扭体反应进行镇痛实验。结果：小茴香挥发油能显著抑制上述各种动物模型的炎症反应及醋酸引起的小鼠扭体反应。结论：小茴香挥发油具有抗炎和镇痛作用。     

小茴香挥发油的抗炎镇痛作用

目的:研究小茴香挥发油的抗炎、镇痛作用,为指导临床合理用药提供科学依据。方法:应用二甲苯致小鼠耳廓肿胀、蛋清致大鼠足肿胀2种动物模型进行抗炎药效学实验;采用醋酸致小鼠扭体反应进行镇痛实验。结果:小茴香挥发油能显著抑制上述各种动物模型的炎症反应及醋酸引起的小鼠扭体反应。结论:小茴香挥发油具有抗炎和镇痛作用。     

前列腺Ⅰ号抗炎镇痛作用以及抗前列腺炎的实验研究

采用热板法和扭体法观察前列腺Ⅰ号抗炎、镇痛以及抗大鼠实验性前列腺炎的作用。采用小鼠耳廓二甲苯制炎法观察抗炎作用,前列腺炎模型采用向大鼠前列腺组织内注入角叉菜胶的方法。结果显示前列腺Ⅰ号能减少小鼠扭体反应数,延长小鼠热板法引起的痛反应潜伏期,对小鼠耳廓肿胀有明显的抑制作用,前列腺炎模型试验中,前列腺液检查卵磷脂小体明显增加,白细胞数降低。因此前列腺Ⅰ号具有明显的抗炎、镇痛以及抗前列腺炎的作用。     

槐角黄酮栓抗炎、止血、抗溃疡作用的实验研究

目的:研究槐角黄酮栓的抗炎、止血、抗溃疡作用。方法:从中药槐角中提取总黄酮,与脂肪酸甘油酯混合后用热融法制成栓剂。采用小鼠耳廓肿胀法、滤纸肉芽肿法、腹腔毛细血管通透性试验和角叉菜胶致大鼠足跖肿胀法观察槐角黄酮栓的抗炎作用;通过玻璃毛细管法和断尾法测定小鼠出、凝血时间,评价槐角黄酮栓的止血效果。复制大鼠直肠损伤模型,直肠给药治疗,观察伤口愈合情况并HE染色,评价槐角黄酮栓的促愈合作用。结果:槐角黄酮栓具有明显的抗炎、抗溃疡作用,并能缩短小鼠出、凝血时间,提高直肠创面愈合率。结论:槐角黄酮栓制备工艺简单,具有较好的抗炎、止血、抗溃疡作用。     

6种秦艽镇痛和抗炎活性的比较

目的:比较六种秦艽甲醇提取物抗炎镇痛活性。方法:采用二甲苯致小鼠耳廓肿胀、棉球诱发小鼠肉芽肿试验、热板法和冰醋酸致小鼠扭体试验。结果:粗茎秦艽对由于腹腔注射冰醋酸引起急性腹膜炎而产生持久的疼痛刺激的镇痛效果最好,达乌里秦艽对热刺激引起的疼痛的缓解效果最好,大叶秦艽对二甲苯引起的炎症反应的抑制效果最佳,而麻花秦艽对棉球诱导的慢性炎症反应的抑制率最高。分析结果显示,黄管秦艽和管花秦艽在镇痛、抗炎活性上与上述4种药典收录的秦艽没有显著性差异。结论:黄管秦艽和管花秦艽在镇痛抗炎方面可作为秦艽的替代品。     

LIG抗炎,解热活性的研究

本文研究了当归提取物LIG的抗炎,解热活性.研究发现LIG(2.5,5,10 mg/kg)对角叉菜和右旋糖苷诱导的足肿胀的抑制率分别为22.2%,49.4%,76.5%和20.8%,44.2%,75.3%(P＜0.001);对棉球肉芽肿的抑制率为29.2%,44.9%,58.8%(P＜O.001);对小鼠白细胞迁移的抑制率为20.7%,35.6%,48.2%(P＜0.001).此外,LIG可降低啤酒酵母导致的高热(P＜0.001).并且,LIG的抗炎,解热作用均成剂量依赖性.以上结果显示LIG具有非常强的抗炎,解热活性.     

Anti-inflammatory and analgesic activity of ononitol monohydrate isolated from Cassia tora L. in animal models

Ononitol monohydrate (OM) was isolated from Cassia tora L. leaves. The anti-inflammatory and analgesic activities of OM have been examined in male Wistar rats and mice. The efficacy of OM against inflammation was studied by using carrageenan-induced paw oedema, croton oil-induced ear oedema, acetic acid-induced vascular permeability, cotton pellet-induced granuloma and adjuvant-induced arthritis. The analgesic activity of OM was assessed using the acetic acid-induced abdominal constriction response, formalin-induced paw licking response and the hot-plate test. In acute type inflammation models, maximum inhibitions of 50.69 and 61.06% (P < .05) were noted with 20 mg/kg of OM in carrageenan-induced hind paw oedema and croton oil-induced ear oedema, respectively. Treatment of OM (20 mg/kg) meaningfully (P < .05) reduced the granuloma tissue formation by cotton pellet study at a rate of 36.25%. OM (20 mg/kg) inhibited 53.64% of paw thickness in adjuvant-induced arthritis model. OM has also been produced significant (P < .05) analgesic activity in acetic acid-induced abdominal constriction response, formalin-induced paw licking response and in hot-plate test suggesting its peripheral and central analgesic potential. The outcomes of the present study proposed that OM influenced on the anti-inflammatory and analgesic activities.     

横断山区伞形科4种7个居群植物的核型研究

对横断山区伞形科棱子芹属2种植物(松潘棱子芹Pleurospermum franchetianumHemsl.和西藏棱子芹Pleurospermum hookeriC.B.Clarke var.thomsoniiC.B.Clarke)和茴芹属2种植物(异叶茴芹Pimpinella diversi-foliaDC.和锐叶茴芹Pimpinella argutaDiels)共7个居群进行体细胞染色体数目观察和核型比较分析,结果表明,棱子芹属和茴芹属植物属内种间染色体基数存在差异,其中松潘棱子芹为2n=2x=18=16sm 2st,西藏棱子芹为2n=2x=22=16m 6sm;茴芹属光果组中锐叶茴芹为2n=2x=22=22m,毛果组中异叶茴芹为2n=18=18st或2n=18=2sm 16st.松潘棱子芹、西藏棱子芹、锐叶茴芹的染色体数目和核型均为首次报道,从而为棱子芹属和茴芹属的分类和演化研究提供细胞学依据.     

Design,synthesis, and pharmacological evaluation of novel oxadiazole and oxadiazoline analogs as anti-inflammatory agents

Two novel series of oxadiazole and oxadiazoline analogs possessing an indole nucleus were synthesized for their potential anti-inflammatory activity. The structures of the compounds were elucidated by elemental and spectral (IR, ¹H-NMR, ¹³C-NMR, and MS) analysis. Most of the test compounds demonstrated appreciable anti-inflammatory activities. The anti-inflammatory activity of oxadiazoles at doses of 100?mg/kg was shown by their ability to provide 27–66%, 14–32%, and 20-51%. protection against carrageenan-induced rat paw edema, moist cotton pellet-induced, and dry cotton pellet-induced granuloma, respectively. On the other hand, the anti-inflammatory properties of oxadiazolines at doses of 100?mg/kg were reflected by their ability to provide 20-56%, 11–26%, and 25–47% protection against carrageenan-induced rat paw edema, moist cotton pellet-induced, and dry cotton pellet-induced granuloma, respectively. The ulcerogenic potential of the compounds was determined. Structure–activity relationships among synthesized compounds were also established.     

小叶榕不同提取物的主要药效学研究

为研究小叶榕不同提取部位的镇咳、抗炎作用,采用氨水致咳小鼠动物模型、枸橼酸致咳豚鼠动物模型来评价小叶榕不同提取部位的镇咳作用;采用二甲苯滴耳廓致肿胀的小鼠动物模型、大鼠棉球肉芽肿动物模型来评价小叶榕不同提取部位的抗炎作用。结果表明:小叶榕醇沉不溶物能延长小鼠和豚鼠的咳嗽潜伏期,但不能减少咳嗽次数,小叶榕水提物、水提醇沉溶解物、大孔吸附树脂吸附洗脱物能延长小鼠和豚鼠的咳嗽潜伏期,并能减少咳嗽次数,其中水提醇沉溶解物镇咳作用最强;小叶榕醇沉不溶物不能减轻二甲苯引起的小鼠耳廓肿胀的程度,能减轻大鼠棉球肉芽肿足跖肿胀的程度,但效果不明显,小叶榕水提物、水提醇沉溶解物、大孔吸附树脂吸附洗脱物均能明显减轻二甲苯引起的小鼠耳廓肿胀的程度,并能明显减轻大鼠棉球肉芽肿足跖肿胀的程度。这表明国产小叶榕不同提取部位具有明显的镇咳、抗炎作用。     

Anti-inflammatory, antiarthritic and analgesic activity of a herbal formulation (DRF/AY/4012)

Anti-inflammatory, antiarthritic and analgesic effect of a herbal product (DRF/AY/4012) was evaluated in animal models. Herbal product treatment induced a dose dependent anti-inflammatory activity in acute inflammatory models (carrageenin and egg-albumin induced rat hind paw edema). It also elicited promising anti-inflammatory activity in chronic inflammatory models (cotton pellet granuloma and Freund's adjuvant induced polyarthritis in rats). Further, the product inhibited the increased level of serum lysosomal enzyme activity viz. serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase and the lipid peroxidation in liver. In Freund's adjuvant induced polyarthritis, herbal product reduced the increased level of hydroxy proline, hexosamine and total protein content in edematous tissue. The product also exhibited mild to moderate analgesic activity in acetic acid induced writhing in mice. The LD50 value of the herbal product was more than 16 gm/kg by oral route in mice. The product has distinct advantages over the existing agents and deserves further developmental studies.     

Anti-inflammatory and analgesic activity of Indian Hypericum perforatum L

A standardised 50% aqueous ethanolic extract of the Indian variety of Hypericum perforatum (IHp) was examined for its putative anti-inflammatory and analgesic activity at the doses of 100 and 200 mg/kg, po. The experimental paradigms used were carrageenan induced pedal edema and cotton pellet induced granuloma for anti-inflammatory activity, whereas the tail flick, hot plate and acetic acid induced writhing methods were used to asses analgesic activity. Indomethacin (20 mg/kg, ip) was used as the standard anti-inflammatory drug. Pentazocine (10 mg/kg, ip) and aspirin (25 mg/kg, ip), both clinically used analgesics, were used as standard analgesics for comparison. IHp extract showed significant anti-inflammatory and analgesic activity at both dose levels, in all the paradigms used. Additionally, IHp potentiated the anti-inflammatory activity of indomethacin and analgesic activities of pentazocine and aspirin.