沙苑子的化学成分和药理作用

沙苑子主要含有黄酮类、三萜类、有机酸类、氨基酸、多肽、蛋白质、鞣质、甾醇、微量元素等。具有改善血液流变学指标、降低血压、调节血脂、抑制血小板聚集、保护肝脏、增强免疫功能、抗炎、镇痛及调节中枢神经系统等作用。     

沙苑子的保健功效与药膳

对我国传统中草药沙苑子的植物形态及分布,历代本草利用,化学成分,药理、毒理作用和保健药膳进行了论述.     

樟子松乙醇提取物体外抗氧化活性研究

测定了樟子松球果、树皮、树叶、木质部4个部位提取物的总抗氧化能力、总还原力、二苯代苦味酰基（DPPH·）清除能力、羟基自由基（·OH）清除能力、超氧阴离子（O^·₂）清除能力5个指标,并以抗坏血酸（Vc）为阳性对照评价从樟子松4个部位提取的松多酚体外抗氧化活性。结果表明：（1）从樟子松树皮中提取的樟子松多酚表现出较强的抗氧化能力,在较高浓度下与抗氧化剂（Vc）相差较小;从樟子松球果中提取的樟子松多酚也表现出较强的还原力,甚至超过阳性对照抗氧化剂Vc;（2）从樟子松树皮中提取的樟子松多酚在二苯代苦味酰基（DPPH·）清除能力、羟基自由基（·OH）清除能力上均为三者之中最高的,但都不超过抗氧化剂（Vc）的抗氧化能;（3）超氧阴离子（O^·₂）清除能力。三者的清除率均为负值,说明该方法测定超氧阴离子（O^·₂）清除率存在干扰因素,无法比较三者在这一指标上的能力大小。上述结果表明樟子松乙醇提取物具有较强的抗氧化能力,可作为天然的抗氧化剂。     

以沙苑子苷为对照品测定沙苑子总黄酮含量

目的 :以沙苑子苷为对照品测定沙苑子总黄酮含量。方法 :80 %乙醇提取液 ,2 6 6nm测定紫外吸收度。结果 :总黄酮含量为 2 1.5mg/g ,RSD =1.0 0 % ;回收率 95 .4 % ,RSD =2 .2 7% ,线性范围 4～ 2 4ug/ml。结论 :本法测定沙苑子总黄酮含量 ,操作简便 ,稳定性好 ,可用于沙苑子的质量控制。     

固相小柱对沙苑子总黄酮萃取方法的研究

目的:以沙苑子总黄酮为研究对象,考察固相萃取小柱的洗脱条件,确定固相萃取沙苑子总黄酮的实验方法。方法:以不同浓度的甲醇对不同填料的固相小柱进行洗脱,将洗脱液在岛津VP-ODS(150 mm×4.6 mm,5μm)柱上进行高效液相色谱分析。通过比较不同浓度及体积甲醇的萃取效率,确定沙苑子苷在固相萃取小柱上的洗脱条件。结果:用正相氨基小柱以50%甲醇洗脱效果好,回收率可达100%。结论:经试验,正相氨基小柱对沙苑子苷的萃取回收率约为100%,适用于沙苑子总黄酮中沙苑子苷的分析。     

凤仙花不同部位乙醇提取物多酚、黄酮含量测定及抗氧化活性研究

采用55%的乙醇分别回流提取凤仙花的茎、叶、花中的活性物质,采用比色法测定各部位的多酚及总黄酮含量,测定各提取物对DPPH、羟自由基、超氧阴离子自由基的清除能力和还原力等抗氧化活性指标。结果表明,凤仙花各部位的多酚(以没食子酸计)、总黄酮(以芦丁计)含量由高到低依次为凤仙花的叶、花、茎,多酚含量分别为72.81、66.07、33.07 mg/m L,总黄酮含量分别为112.13、108.06、30.62 mg/m L。各种提取物均有不同程度的抗氧化活性,且抗氧化活性与提取物浓度存在量效关系,其中叶提取物清除DPPH自由基、羟自由基、超氧阴离子自由基活性以及还原力均最高,本研究为凤仙花作为低毒高效的天然抗氧化剂应用提供了依据。     

加拿大一枝黄花提取物的抗氧化活性研究

为进一步开发和利用加拿大一枝黄花(Solidago canadensis),该研究采用氯化铝显色法和福林酚法测定加拿大一枝黄花乙醇提取物及其不同极性萃取物中的总黄酮和总酚的含量;以抗坏血酸(Vitamin C,Vc)和二丁基羟基甲苯(BHT)为阳性对照,应用2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)、1,1-二苯基苦基苯肼(DPPH)自由基清除体系、铁离子还原能力(FRAP)法和抗氧化能力指数(ORAC)法研究其体外抗氧化活性。结果表明:乙酸乙酯萃取物中的总黄酮(202.45 mg·g~(-1))和总酚(485.94 mg·g~(-1))含量最高,且其抗氧化活性最强,并强于阳性对照Vc(P0.05)。因而,加拿大一枝黄花乙酸乙酯萃取物将有可能成为一种潜在的天然高效抗氧化剂,具有广泛的应用前景。     

沙苑子的组织培养与快速繁殖

以扁茎黄芪的干燥成熟种子沙苑子为材料进行扁茎黄芪的组织培养研究.结果表明:沙苑子的发芽培养基为 MS,扁茎黄芪继代增殖最适培养基为:MS 6-BA3.0 mg/L NAA 0.5 mg/L,平均增殖系数达到 4.5,平均苗高达3.5 cm;生根最适培养基为:1/2MS NAA0.05 mg/L,生根数为4～6条,生根率达到100%.     

李氏块菌提取物抗氧化活性的研究

对李氏块菌Tuber liyuanum子实体的不同提取物,包括甲醇提取物(ME)、乙醇提取物(EE)、丙酮提取物(AE)、正丁醇提取物(BAE)和乙酸乙酯提取物(EAE),进行清除DPPH自由基、羟基自由基、超氧阴离子自由基、铁离子螯合能力、还原力以及总酚含量的测定和研究,发现ME对DPPH自由基、超氧阴离子自由基清除及还原力活性最高,EC50值分别为23.37mg/m L、11.65mg/m L和24.47mg/m L;EE对羟基自由基清除和铁离子螯合能力活性最高,EC50值分别为7.24mg/m L和小于0.5mg/m L;ME的总酚含量最高(3.08mg GAE/g提取物),其次是EE(1.34mg GAE/g提取物),提取物总酚含量与抗氧化活性呈现出一定的正相关性。     

印度块菌提取物抗氧化活性的研究

对印度块菌Tuber indicum子实体的提取物,包括55%乙醇粗提物(ECE)、石油醚提取物(PEF)和乙酸乙酯提取物(EAF)的清除DPPH自由基和羟基自由基能力、铁离子鳌合能力以及各提取物的总酚含量等进行了研究和测定,结果显示,3种提取物的清除自由基能力和铁离子鳌合能力具有显著差异(P0.05);ECE对DPPH自由基的清除活性最高,其EC50值为1.61g/L;EAF对羟基自由基及铁离子表现出较强的清除或螯合的能力,其EC50值分别为3.31g/L和0.70g/L;EAF的总酚含量(2.964mg GAE/g提取物)最高,其次是ECE,总酚含量为(2.618mg GAE/g提取物);PEF的清除自由基和铁离子鳌合能力较差,其总酚含量也最低(1.124mg GAE/g提取物);总酚含量与印度块菌提取物清除自由基以及鳌合铁离子的能力密切相关。     

Analytical Profiling of Phenolic Compounds in Extracts of Three Cistus Species from Sardinia and Their Potential Antimicrobial and Antioxidant Activity

The Cistaceae family is well represented in Sardinia, and the Cistus genus is widely used in traditional medicine. Nowadays only few studies have been performed on this genus vegetating in Sardinia in spite of its ethnobotanical importance. Moreover, in the past there have been conflicting opinions among botanists for the exact assignment of the species growing in Sardinia. We started several years ago to carried out studies on this genus and in the present study was to evaluate the in vitro activity of several samples of Cistus salvifolius L., Cistus monspeliensis L., and Cistus albidus L. collected in Sardinia as antimicrobial agents against Escherichia coli, Staphylococcus aureus, and four Candida species and their antioxidant activity using DPPH, ABTS, and FRAP assays. Furthermore, the phenolic content and composition of the extracts were first evaluated. Using statistical multivariate analysis on the complete metabolomics profile of all Cistus species growing wild in Sardinia, we confirmed the botanical classification, and we observed an interesting correlation between metabolomics profile and antioxidant activity.     

蓝莓果渣提取物总酚含量及抗氧化活性研究

研究了蓝莓果渣提取物总酚含量及其抗氧化活性。分别采用水、40%乙醇及纤维素酶辅助乙醇超声提取蓝莓果渣,并用Folin-Ciocalteu试剂对3种提取物的总酚含量进行评估;并采用DPPH清除实验及O2—.清除实验对3种提取物的抗氧化活性进行研究。实验结果表明,纤维素酶辅助超声提取蓝莓果渣的总酚含量最高,可达425.36±15.21 mg GAE.100 g-1DW,远远高于水提物(169.46±9.75 mg GAE.100 g-1DW)及醇提物(218.39±12.54mg GAE.100 g-1DW)中的总酚含量。且纤维素酶辅助乙醇超声提取物对DPPH的清除能力为2.67±0.13 gVc.100 g-1DW,对O—.2的清除能力2.48±0.14 g Vc.100 g-1DW,明显好于醇提物及水提物抗氧化活性。     

固相萃取-高效液相色谱法测定沙苑子苷含量

目的：建立沙苑子总黄酮中沙苑子苷的含量测定方法。方法：用以氨基为固定相的固相萃取柱对沙苑子总黄酮进行纯化,洗去杂质后的洗脱液进高效液相色谱仪测定。色谱柱为VP—ODS（150mm×4．6mm,5um）,流动相为甲醇：1％HAC溶液（41：59）;流速1．2mL／min;检测波长266nm。结果：沙苑子苷在5—25μg／mL范围内呈良好的线性关系,加样回收率为102．5％,RSD2．3％。结论：本法灵敏、快速、准确,可用于测定沙苑子总黄酮中沙苑子苷的含量。     

Enhanced Phenolic Diterpenes Antioxidant Levels Through Non-transgenic Approaches

Plant-derived antioxidants are essential in our diet, and antioxidant composition is a key determinant of the quality of plant extracts of interest to the pharmaceutical and food industries. By using carnosic acid as an example of a key antioxidant constituent of rosemary and sage extracts, we discuss the importance of studying non-transgenic approaches to enhancing antioxidant levels in plants and improving the antioxidant composition of plant extracts. In contrast to other terpenoids or phenolic compounds, carnosic acid has only been found in some Labiatae species, such as rosemary and sage. Carnosic acid has medicinal properties; it is a potent antioxidant and protects skin cells against UV-A radiation and cancer. Furthermore, it has been used as a preservative in food and non-food products, displaying important antimicrobial effects. However, the key steps involved in its biosynthesis remain largely unknown, and thus non-transgenic approaches are required to increase its level in plant extracts. Dried rosemary or sage leaves can contain between 0.1% and 7% carnosic acid, depending on the species and variety, but also on plant growth conditions, sample treatment and mode of extract preparation. Furthermore, leaf age, salinity and ionic interactions can also have a significant effect on biosynthesis and therefore have a strong impact on the total antioxidant potential of rosemary and sage extracts. Non-transgenic approaches, used in these or other species, can significantly increase antioxidant levels and therefore provide very significant improvements in the quality of several botanical extracts used in industry, and can be applied as either an alternative or a complement to transgenic approaches.

Enhanced Phenolic Diterpenes Antioxidant Levels Through Non-transgenic Approaches

All authors

T. Tounekti &; S. Munné-Bosch

https://doi.org/10.1080/07352689.2012.696457

Published online:

26 October 2012

FIG. 1 Chemical structure of synthetic and natural plant-derived antioxidants. Note that the hydroxyl groups in the ortho-position at C₁₁ and C₁₂ of the carnosic acid molecule provide high antioxidant properties (color figure available online).     

15.

樟芝发酵液和菌丝体提取物抗氧化性能研究     

韩金龙  赵培城  毛建卫  刘士旺 《微生物学杂志》2014,(5):49-54

采用甲醇、乙醇、异丙醇对樟芝发酵液和菌丝体进行提取,对其不同提取物进行还原力,DPPH、羟基自由基、超氧自由基、过氧化氢清除能力,亚铁离子螯合能力等抗氧化能力的测定。比较发酵液提取物和菌丝体提取物的抗氧化效果,比较不同提取剂提取物的抗氧化效果。结果显示:(1)发酵液提取物还原力最高的为异丙醇提取物,亚铁离子螯合能力最强的为乙醇提取物;DPPH清除能力乙醇和异丙醇提取物最高都达到97.88%;羟基自由基清除能力最高的为乙醇提取物,达到84.22%;3种提取物的超氧自由基清除能力都较高,具有最高清除率的异丙醇提取物达到94.89%;过氧化氢清除能力最高的为乙醇提取物的97.53%。(2)菌丝体提取物还原力最高和亚铁离子螯合能力最强的均为乙醇提取物;DPPH清除能力最高的为乙醇提取物的89.95%;3种提取物的羟基自由基清除能力普遍偏低,最高的仅为异丙醇提取物的37.28%;超氧自由基清除能力则普遍较高,最高为甲醇提取物的90.05%;过氧化氢清除能力最高的为乙醇和异丙醇提取物,最高清除率均为95.06%。抗氧化性能的研究比较,为进一步开发利用樟芝提供了依据。     

16.

沙棘籽提取物抗氧化活性与酚类组成的研究   总被引：10，自引：0，他引：10  

樊金玲  丁霄霖 《天然产物研究与开发》2006,18(4):529-534,554

采用卵磷脂脂质体体系比较了沙棘籽不同溶剂提取物的抗氧化活性,对其中的抗氧化活性成分进行了分析。试验结果表明:当浓度为250μg/mL时,提取物对共轭二烯氢过氧化物的抑制率为0~90.1%,对丙二醛的抑制率为0~88.6%。70%丙酮提取物具有最强的抗氧化活性、清除DPPH自由基能力和还原力。各提取物中主要的酚类物质为原花色素,抗氧化活性与原花色素含量有很好的相关性。     

17.

Phenolic Profiling,Antioxidant, and Antibacterial Activities of Selected Medicinal Plants from Tunisia     

Hania Hamrouni  Khadija Ben Othman  Hasnia Benmoussa  Sourour Idoudi  Hanen Najjaa  Mohamed Neffati  Walid Elfalleh 《化学与生物多样性》2023,20(8):e202300265

Phytochemical screening of aqueous extract from six medicinal wild plants grown in South-eastern of Tunisia: Atriplex halimus, Teucrium polium, Moricandia arvensis, Deverra tortuoa, Haplophyllum tuberculatum and Polygonum equisetiforme were evaluated. Both decoction and ultrasound assisted extraction were used. Antioxidant, antibacterial proprieties, and phenolic profiling, using LC-ESI-MS method, were assessed. Total polyphenols, flavonoids, and condensed tannins contents ranged from 7.47±0.19 to 22.25±0.49 mg GAE/g Dw, 5.47±0.06 to 7.55±0.07 mg RE/g Dw, and 0.33±0.02 to 19.43±0.64 mg TAE/g Dw, respectively. Moreover, the reducing power and DPPH tests showed that P. equisetiforme (EC₅₀: 12.50±0.50 μg/ml; DPPH⋅⁺: 213.49±4.24 mg TEAC/g DW), T. polium (EC₅₀: 25.00±1.00 μg/ml; DPPH⋅⁺: 181.39±9.47 mg TEAC/g DW) as well as H. tuberculatum (EC₅₀: 56.25±0.25 μg/ml; DPPH⋅⁺: 177.83±5.85 mg TEAC/g DW) extracts were the most effective natural antioxidants. For anti-bacterial activity, the ultrasonic extract of H. tuberculatum showed the highest activity against both P. aeruginosa (13.50±0.71 mm) and S. aureus (13.00±0.00 mm) at 10 mg/ml. Furthermore 24 phenolic compounds were identified, with predominance of quinic acid, gallic acid, protocatechuic acid, syringic acid, p-coumaric acid, trans-ferulic acid, catechin (+), trans-cinnamic and silymarin. These results were further consolidated by to heatmap clustering with P. equisetiforme, H. tuberculatum, T. polium as the main antioxidant and antibacterial sources which supports their domestication and industrial use.     

18.

Antioxidant Activity,Total Phenolic,Tannin, and Flavonoid Content of Five Plants Used in Traditional Medicine in Penjamo,Guanajuato     

Jahir Antonio Barajas-Ramírez  Angel Humberto Cabrera-Ramírez  Victoria Guadalupe Aguilar-Raymundo 《化学与生物多样性》2023,20(1):e202200834

In Mexico, plants are commonly used to alleviate various ailments, including controlling some chronic degenerative diseases through the regular consumption of decoctions, infusions, and teas. However, there is little scientific evidence consolidating traditional medicine within health systems. Therefore, this work determined the phytochemical profile of the most used plants to treat various ailments (Cedro rojo, Cancerina, Ortiguilla, Hierba de la golondrina, Hierba de arlomo) and their general consumption as infusions. Aqueous and ethanolic extracts were generated, while the phytochemical compound content in the extracts obtained was quantified. The results indicate that the ethanolic extracts showed the highest phenolic compound and tannin content, with the highest contents for Cedro rojo (831.04 mg L⁻¹) and Cancerina (864.80 mg L⁻¹). The antioxidant activity was also determined, and a significant difference was observed (p<0.05). The extracts with the highest antioxidant capacity were the ethanolic extracts ranging from 250 to 907 μMET mL⁻¹, while the aqueous extracts ranged from 112 to 390 μMET mL⁻¹. The compounds identified by high-performance liquid chromatography characterization on the aqueous extracts highlighted the presence of chlorogenic acid>cinnamic acid>quercetin. In ethanolic extracts, the presence of chlorogenic acid>cinnamic acid>quercetin>gallic acid>ferulic acid>coumaric acid was highlighted. The correlation between bioactive compounds, type of extract, and antioxidant activity suggests a significant affinity of these phytochemical compounds for the ethanol solvent. The results indicate that these plants are good sources of antioxidant phenolics and can be incorporated for use as functional beverages. However, more studies are needed to corroborate their beneficial effect.     

19.

欧李果实酚类物质及抗氧化活性特征研究     

邵郅胜  张薇  孙聪  郭金丽 《西北植物学报》2023,43(8):1405-1414

以内蒙古地区36种欧李优系资源果实为试验材料,分析欧李果实品质性状的9项指标含量差异及变异系数探究其多样性,通过相关性、主成分、聚类分析对其品质性状进行综合评价,筛选酚类品质优异的欧李优系资源,为内蒙古欧李种质资源创新利用提供理论依据。结果表明,(1)9项品质性状指标变异系数为5.91%~31.03%,总黄酮最高,可滴定酸最低。(2)欧李果实3种酚类物质与总还原力、·OH清除率、DPPH·清除率及果肉色泽多表现极显著正相关(P<0.01)。(3)通过主成分分析提取的3个主成分累计贡献率为73.54%,分别为酚类因子、酸度因子和总抗氧化因子;建立欧李种质综合评价数学模型:F=[10×(46.213×F_(1)+15.132×F_(2)+12.197×F_(3))/73.542]+60,综合得分最高为MY37(84.53),综合表现最差为MY9(29.61)。(4)系统聚类评价,将36份优系资源聚为4大类,其中第Ⅰ类群综合表现最优,分别为MY5、MY36、MY37、MY38、MY41、MY47、MY50,为酚类物质积累型,多为红色果肉,酸含量适中,同时这7份优系资源综合得分均在前10位,此类群可作为高酚类品质欧李种质资源。     

20.

红花桑寄生叶提取物的抗氧化活性及酚类物质分析   总被引：4，自引：0，他引：4  

赖京菁  陈炳华  连俊蕊  陈玉芳  后望  黄张关 《亚热带植物科学》2008,37(3):38-42

采用DPPH法、TEAC法、FRAP法对红花桑寄生叶不同溶剂提取物的抗氧化活性进行体外评价,并测定其总酚、总黄酮含量。结果表明,溶剂种类对红花桑寄生叶提取物的得率、总酚、总黄酮及抗氧化活性影响显著。在3种评价方法中,不同溶剂提取物的抗氧化活性均表现出不同程度的量效依赖关系。3种溶剂提取物的抗氧化活性强弱依次为丙酮提取物 >甲醇提取物 >水提取物,其中80%丙酮提取物(总酚含量最高,达276.83mg/g)抗氧化活性最强,清除DPPH自由基能力EC₅₀值为0.247,FRAP值(FeSO₄ mmol/100g)为115.81,浓度为1.0mg/ml时,TEAC值为2.04。     

樟芝发酵液和菌丝体提取物抗氧化性能研究

采用甲醇、乙醇、异丙醇对樟芝发酵液和菌丝体进行提取,对其不同提取物进行还原力,DPPH、羟基自由基、超氧自由基、过氧化氢清除能力,亚铁离子螯合能力等抗氧化能力的测定。比较发酵液提取物和菌丝体提取物的抗氧化效果,比较不同提取剂提取物的抗氧化效果。结果显示:(1)发酵液提取物还原力最高的为异丙醇提取物,亚铁离子螯合能力最强的为乙醇提取物;DPPH清除能力乙醇和异丙醇提取物最高都达到97.88%;羟基自由基清除能力最高的为乙醇提取物,达到84.22%;3种提取物的超氧自由基清除能力都较高,具有最高清除率的异丙醇提取物达到94.89%;过氧化氢清除能力最高的为乙醇提取物的97.53%。(2)菌丝体提取物还原力最高和亚铁离子螯合能力最强的均为乙醇提取物;DPPH清除能力最高的为乙醇提取物的89.95%;3种提取物的羟基自由基清除能力普遍偏低,最高的仅为异丙醇提取物的37.28%;超氧自由基清除能力则普遍较高,最高为甲醇提取物的90.05%;过氧化氢清除能力最高的为乙醇和异丙醇提取物,最高清除率均为95.06%。抗氧化性能的研究比较,为进一步开发利用樟芝提供了依据。     

沙棘籽提取物抗氧化活性与酚类组成的研究

采用卵磷脂脂质体体系比较了沙棘籽不同溶剂提取物的抗氧化活性,对其中的抗氧化活性成分进行了分析。试验结果表明:当浓度为250μg/mL时,提取物对共轭二烯氢过氧化物的抑制率为0~90.1%,对丙二醛的抑制率为0~88.6%。70%丙酮提取物具有最强的抗氧化活性、清除DPPH自由基能力和还原力。各提取物中主要的酚类物质为原花色素,抗氧化活性与原花色素含量有很好的相关性。     

Phenolic Profiling,Antioxidant, and Antibacterial Activities of Selected Medicinal Plants from Tunisia

Phytochemical screening of aqueous extract from six medicinal wild plants grown in South-eastern of Tunisia: Atriplex halimus, Teucrium polium, Moricandia arvensis, Deverra tortuoa, Haplophyllum tuberculatum and Polygonum equisetiforme were evaluated. Both decoction and ultrasound assisted extraction were used. Antioxidant, antibacterial proprieties, and phenolic profiling, using LC-ESI-MS method, were assessed. Total polyphenols, flavonoids, and condensed tannins contents ranged from 7.47±0.19 to 22.25±0.49 mg GAE/g Dw, 5.47±0.06 to 7.55±0.07 mg RE/g Dw, and 0.33±0.02 to 19.43±0.64 mg TAE/g Dw, respectively. Moreover, the reducing power and DPPH tests showed that P. equisetiforme (EC₅₀: 12.50±0.50 μg/ml; DPPH⋅⁺: 213.49±4.24 mg TEAC/g DW), T. polium (EC₅₀: 25.00±1.00 μg/ml; DPPH⋅⁺: 181.39±9.47 mg TEAC/g DW) as well as H. tuberculatum (EC₅₀: 56.25±0.25 μg/ml; DPPH⋅⁺: 177.83±5.85 mg TEAC/g DW) extracts were the most effective natural antioxidants. For anti-bacterial activity, the ultrasonic extract of H. tuberculatum showed the highest activity against both P. aeruginosa (13.50±0.71 mm) and S. aureus (13.00±0.00 mm) at 10 mg/ml. Furthermore 24 phenolic compounds were identified, with predominance of quinic acid, gallic acid, protocatechuic acid, syringic acid, p-coumaric acid, trans-ferulic acid, catechin (+), trans-cinnamic and silymarin. These results were further consolidated by to heatmap clustering with P. equisetiforme, H. tuberculatum, T. polium as the main antioxidant and antibacterial sources which supports their domestication and industrial use.     

Antioxidant Activity,Total Phenolic,Tannin, and Flavonoid Content of Five Plants Used in Traditional Medicine in Penjamo,Guanajuato

In Mexico, plants are commonly used to alleviate various ailments, including controlling some chronic degenerative diseases through the regular consumption of decoctions, infusions, and teas. However, there is little scientific evidence consolidating traditional medicine within health systems. Therefore, this work determined the phytochemical profile of the most used plants to treat various ailments (Cedro rojo, Cancerina, Ortiguilla, Hierba de la golondrina, Hierba de arlomo) and their general consumption as infusions. Aqueous and ethanolic extracts were generated, while the phytochemical compound content in the extracts obtained was quantified. The results indicate that the ethanolic extracts showed the highest phenolic compound and tannin content, with the highest contents for Cedro rojo (831.04 mg L⁻¹) and Cancerina (864.80 mg L⁻¹). The antioxidant activity was also determined, and a significant difference was observed (p<0.05). The extracts with the highest antioxidant capacity were the ethanolic extracts ranging from 250 to 907 μMET mL⁻¹, while the aqueous extracts ranged from 112 to 390 μMET mL⁻¹. The compounds identified by high-performance liquid chromatography characterization on the aqueous extracts highlighted the presence of chlorogenic acid>cinnamic acid>quercetin. In ethanolic extracts, the presence of chlorogenic acid>cinnamic acid>quercetin>gallic acid>ferulic acid>coumaric acid was highlighted. The correlation between bioactive compounds, type of extract, and antioxidant activity suggests a significant affinity of these phytochemical compounds for the ethanol solvent. The results indicate that these plants are good sources of antioxidant phenolics and can be incorporated for use as functional beverages. However, more studies are needed to corroborate their beneficial effect.     

欧李果实酚类物质及抗氧化活性特征研究

以内蒙古地区36种欧李优系资源果实为试验材料,分析欧李果实品质性状的9项指标含量差异及变异系数探究其多样性,通过相关性、主成分、聚类分析对其品质性状进行综合评价,筛选酚类品质优异的欧李优系资源,为内蒙古欧李种质资源创新利用提供理论依据。结果表明,(1)9项品质性状指标变异系数为5.91%~31.03%,总黄酮最高,可滴定酸最低。(2)欧李果实3种酚类物质与总还原力、·OH清除率、DPPH·清除率及果肉色泽多表现极显著正相关(P<0.01)。(3)通过主成分分析提取的3个主成分累计贡献率为73.54%,分别为酚类因子、酸度因子和总抗氧化因子;建立欧李种质综合评价数学模型:F=[10×(46.213×F_(1)+15.132×F_(2)+12.197×F_(3))/73.542]+60,综合得分最高为MY37(84.53),综合表现最差为MY9(29.61)。(4)系统聚类评价,将36份优系资源聚为4大类,其中第Ⅰ类群综合表现最优,分别为MY5、MY36、MY37、MY38、MY41、MY47、MY50,为酚类物质积累型,多为红色果肉,酸含量适中,同时这7份优系资源综合得分均在前10位,此类群可作为高酚类品质欧李种质资源。     

红花桑寄生叶提取物的抗氧化活性及酚类物质分析

采用DPPH法、TEAC法、FRAP法对红花桑寄生叶不同溶剂提取物的抗氧化活性进行体外评价,并测定其总酚、总黄酮含量。结果表明,溶剂种类对红花桑寄生叶提取物的得率、总酚、总黄酮及抗氧化活性影响显著。在3种评价方法中,不同溶剂提取物的抗氧化活性均表现出不同程度的量效依赖关系。3种溶剂提取物的抗氧化活性强弱依次为丙酮提取物 >甲醇提取物 >水提取物,其中80%丙酮提取物(总酚含量最高,达276.83mg/g)抗氧化活性最强,清除DPPH自由基能力EC₅₀值为0.247,FRAP值(FeSO₄ mmol/100g)为115.81,浓度为1.0mg/ml时,TEAC值为2.04。