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H. Bobby Fokidis Nancy A. Schable Cris Hagen Travis C. Glenn Thomas S. Risch 《Molecular ecology resources》2003,3(4):616-618
Polymerase chain reaction primers for microsatellite DNA loci (one dinucleotide, four tetranucleotide and two compound) and the conditions necessary to amplify each are described for the southern flying squirrel (Glaucomys volans). These primers were tested on 22 or more individuals from a population at the Savannah River Site in South Carolina. These microsatellite primers yielded a high allelic diversity (6–22 alleles/locus), and moderate to high observed heterozygosities (0.318–0.826). Primers developed for the northern flying squirrel (Glaucomys sabrinus) were also tested for use on G. volans, with only two successful cross amplifications from the seven loci. 相似文献
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A nuclear perspective on endemism in northern flying squirrels (Glaucomys sabrinus) of the Alexander Archipelago, Alaska 总被引:2,自引:0,他引:2
We used microsatellites to examine populationstructure and genetic diversity in northernflying squirrels in the Alexander Archipelagoof Southeast Alaska, with an emphasis on theendemic Prince of Wales flying squirrel(Glaucomys sabrinus griseifrons). Previouswork showed this subspecific designationcoincided with a distinct mitochondrial lineageon eleven islands (the Prince of Wales [POW]complex). To obtain a nuclear perspective onthis lineage and to further investigate geneticdiversity among insular populations, weexamined six microsatellite loci in 233 flyingsquirrels representing eight populations inSoutheast Alaska and a population from interiorAlaska (seven island and two mainlandlocalities). Island populations have lowerheterozygosity and allelic diversity thanmainland populations. Overall, populationpairs show a pattern of isolation by distance,indicating there is little long-distance geneflow across the archipelago. Analyses ofmicrosatellite allele frequencies revealsignificant differences between the POW complexpopulations and others we examined, a findingcongruent with the mitochondrial data. Thepopulation from Mitkof Island, a non-POWcomplex island, also differs significantly fromother populations in allele frequencies. Thesix POW complex populations are geneticallyvery similar, suggesting current or recent geneflow among these islands, while there seems tobe no gene flow between the POW complex andother populations in Southeast Alaska. Ourdata corroborate mitochondrial DNA resultsindicating that G. s. griseifrons isgenetically distinct and suggest a generalpattern of isolation of insular flyingsquirrels in Southeast Alaska. 相似文献
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COLIN J. GARROWAY JEFF BOWMAN† TARA J. CASCADEN‡ GILLIAN L. HOLLOWAY§ CAROLYN G. MAHAN¶ JAY R. MALCOLM MICHAEL A. STEELE GREGORY TURNER†† PAUL J. WILSON‡‡ 《Global Change Biology》2010,16(1):113-121
There is now unequivocal evidence for global climate change; however, its potential impacts on evolutionary processes remain unclear. Many species have responded to contemporary climate change through shifts in their geographic range. This could lead to increased sympatry between recently diverged species; likely increasing the potential for hybridization. Recently, following a series of warm winters, southern flying squirrels ( Glaucomys volans ) in Ontario, Canada rapidly expanded their northern range limit resulting in increased sympatry with the closely related northern flying squirrel ( Glaucomys sabrinus ). This provided the opportunity to test the prediction that contemporary climate change can act as a catalyst creating conditions for the formation of hybrid zones. Following extensive sampling and molecular analyses (nuclear and mitochondrial DNA), we identified the occurrence of hybridization between sympatric G. sabrinus and G. volans . There was evidence of backcrossing but not of extensive introgession, consistent with the hypothesis of recent rather than historic hybridization. To our knowledge, this is the first report of hybrid zone formation following a range expansion induced by contemporary climate change. This is also the first report of hybridization between North American flying squirrel species. 相似文献
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We characterized 23 polymorphic tetranucleotide microsatellite loci for Sacramento perch (Archoplites interruptus). This species is extirpated in its native range, the Sacramento–San Joaquin Delta (California, USA), and is therefore targeted for recovery. A concerted effort is currently underway to re‐establish self‐sustaining populations of Sacramento perch in its native range. These microsatellites will be used to analyse the population structure of the species and, in conjunction with life history and physiological data, develop a comprehensive recovery plan. 相似文献
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Foley JE Nieto NC Clueit SB Foley P Nicholson WN Brown RN 《Journal of wildlife diseases》2007,43(4):684-689
Epidemic typhus, caused by Rickettsia prowazekii, is maintained in a southern flying squirrel (Glaucomys volans) sylvatic cycle in the southeastern United States. The northern flying squirrel (Glaucomys sabrinus) has not been previously associated with R. prowazekii transmission. A second rickettsial pathogen, Anaplasma phagocytophilum, infects dusky-footed woodrats (Neotoma fuscipes) and tree squirrels in northern California. Because northern flying squirrels or their ectoparasites have not been tested for these rickettsial pathogens, serology and polymerase chain reaction (PCR) were used to test 24 northern flying squirrels for R. prowazekii and A. phagocytophilum infection or antibodies. Although there was no evidence of exposure to R. prowazekii, we provide molecular evidence of A. phagocytophilum infection in one flying squirrel; two flying squirrels also were seropositive for this pathogen. Fleas and ticks removed from the squirrels included Ceratophyllus ciliatus mononis, Opisodasys vesperalis, Ixodes hearlei, Ixodes pacificus, and Dermacentor paramapertus. 相似文献
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Within North America, northern (Glaucomys sabrinus) and southern (Glaucomys volans) flying squirrels occupy distinct ranges with limited overlap. Sympatry in northern latitudes coincides with northern hardwood vegetation from Minnesota to New England. Strongyloides robustus is an intestinal parasite that infects both species but appears to be deleterious only to northern flying squirrels. As a result, S. robustus could be a critical determinant of flying squirrel population characteristics in at least some areas of sympatry. However, cold weather could potentially limit the distribution of S. robustus in northern climates. Therefore, we assessed fecal samples from both flying squirrel species to determine the presence of the nematode in Wisconsin. Strongyloides robustus was found in 12 flying squirrel scat samples and infected 52% of southern flying squirrels and 11% of northern flying squirrels. Prevalence of S. robustus infection for northern flying squirrels was substantially lower than previously reported from more southern regions. This is the northernmost documentation of S. robustus in flying squirrels and the first documentation of S. robustus parasitizing flying squirrels in Wisconsin. 相似文献
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X. Domingo-Roura T. Lpez-Girldez M. Shinohara O. Takenaka 《American journal of primatology》1997,43(4):357-360
We describe seven polymorphic microsatellites isolated from a Japanese macaque (Macaca fuscata) genomic library selected for (GT)n content. The primer sets amplified from four to 11 different alleles in a sample of 14 Japanese macaques from nine different sites along the central and southern distribution of the species. These heterologous primers also detected variability in four other cercopithecine species. Am. J. Primatol. 43:357–360, 1997. © 1997 Wiley-Liss, Inc. 相似文献
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Meghan N. Murrant Jeff Bowman Paul J. Wilson 《Biological journal of the Linnean Society. Linnean Society of London》2014,113(4):1126-1135
It can be challenging to understand the evolution of sociality, particularly the occurrence of co‐operation by non‐kin. Southern flying squirrels (Glaucomys volans) are an interesting example of non‐kin co‐operation because of the mutual benefits obtained by social thermoregulation during winter. Because group survival confers benefits to the entire group, flying squirrels may also follow an aggregation economy, whereby co‐operative foraging during winter is advantageous. However, the extent of such social foraging in flying squirrels is unknown. We tested for social foraging of southern flying squirrels, and also for relatedness among foraging groups. To determine the structure of foraging groups, we set up and remotely monitored feeding stations and nest cavities. All squirrels at the study site were tagged with passive integrated transponder (PIT) tags and nests and feeding stations were monitored with automated PIT‐tag recorders for a 24‐month period. Squirrels were found most often foraging alone. Squirrels that were recorded foraging together comprised unrelated individuals that were also found to share nest cavities. Squirrels were also recorded travelling farther distances between nest cavity and feeding station in the winter season than in the summer season, suggesting that, during winter, squirrels trade‐off proximity to food caches for membership in a nest group. Our data suggest that squirrels forage and cache alone in their summer home range and make solitary returns to this summer range to collect their cache during the winter months, despite exhibiting social winter nesting. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 113 , 1126–1135. 相似文献
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COLIN J. GARROWAY JEFF BOWMAN GILLIAN L. HOLLOWAY JAY R. MALCOLM PAUL J. WILSON 《Global Change Biology》2011,17(5):1760-1769
Climate is an important factor limiting species distributions. Historic climate‐change related range movements have modified the genetic diversity of species by the merging and splitting of gene pools and by the effects associated with recurrent founder events. These effects are often inferred, either from retrospective analyses of current genetic patterns or from simulations. Rarely has it been possible for the population genetic effects of range expansion to be examined with contemporaneous demographic data. We characterized the genetic signature of rapid range expansion by southern flying squirrels (Glaucomys volans) and compared these results to a stationary population of the closely related northern flying squirrel (Glaucomys sabrinus) in Ontario, Canada. Samples were taken during an approximately 200 km range expansion by G. volans (1994–2003) and genotyped at 6 (G. sabrinus) and 8 (G. volans) microsatellite loci. For G. volans, but not G. sabrinus, we found evidence of a latitudinal gradient in allele frequencies and a decrease in allelic richness along the axis of expansion. We found no evidence of isolation‐by‐distance in either species or of genetic bottlenecks in the area of G. volans expansion. These results suggest that serial founder events can cause an immediate reduction in genetic diversity following rapid range expansion with high levels of gene flow giving rise to heterogeneity within what would classically be termed panmixia. Given the pace of anthropogenic climate change, and the increasing incidence of range movements in response, this may be an important, immediate consequence of climate change. 相似文献
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M. A. RUSSELLO V. SARANATHAN S. BUHRMAN‐DEEVER J. EBERHARD A. CACCONE 《Molecular ecology resources》2007,7(6):990-992
Microsatellite loci were characterized for the monk parakeet (Myiopsitta monachus) from a GTn‐enriched genomic library. Twelve of 14 microsatellite loci were polymorphic, averaging 6.7 alleles per locus across the 20 individuals genotyped. Mean expected heterozygosity was 0.72, with locus‐specific values ranging from 0.53 to 0.90. An equally high multilocus probability of identity (2.48 × 10?12) was revealed for this set of loci. In addition, all 12 loci were demonstrated to cross‐amplify to varying extents within three additional parrot genera suggesting their potential utility for population‐level studies in a broad range of Neotropical psittacines. 相似文献
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Cynoglossum officinale is a biennial plant pollinated by bumblebees. We developed microsatelllite loci in order to study the population genetic structure and effects of inbreeding in this species. In this paper, we describe nine polymorphic microsatellites for C. officinale. Between two and four alleles per locus were observed in a sample of 20 individuals from one population. Multiplexing allowed the seven most useful loci to be genotyped using three polymerase chain reaction (PCR) reactions. 相似文献
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Hickman CR Peters MB Crawford NG Hagen C Glenn TC Somers CM 《Molecular ecology resources》2008,8(6):1439-1441
We isolated and characterized nine microsatellite loci from the American white pelican, Pelecanus erythrorhynchos. The loci were screened in 23 individuals from the eastern and western populations of North America and were polymorphic, with the number of alleles per locus ranging from two to eight. Polymorphic information content ranged from 0.185 to 0.820 and observed heterozygosity from 0.217 to 0.957. These new loci will provide tools for studies of population structure in this species, thereby aiding conservation planning. 相似文献
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Yuccas and their pollinator moths are a textbook example of mutualism, yet we lack sufficiently variable markers to properly study the population genetics of the plants. We characterized 13 polymorphic microsatellite loci for Yucca filamentosa by screening primers derived from an expressed sequence tag database. We found four to 13 alleles per locus and the observed heterozygosity ranged from 0.31 to 1. These markers will be useful in future ecological studies of Y. filamentosa. 相似文献
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MARY ANN OLSON ROMAN ZAJAC ADALGISA CACCONE MICHAEL A. RUSSELLO 《Molecular ecology resources》2006,6(2):390-392
Eight dinucleotide microsatellite loci were isolated and characterized from Hobsonia florida, a tube‐dwelling ampharetid polychaete. The identified loci were highly polymorphic, with allelic diversity ranging from six to 11 alleles. Levels of expected heterozygosity were 0.52 or greater in all cases, averaging 0.78 across the complete set of loci. Cross‐species amplification was successful in three of the eight loci for one or both of the other species (Melinna cristata and Ampharete acutifrons) tested. Although these novel loci were designed for immediate utility in H. florida population‐level research, these results indicate they may prove useful in studies of other related taxa. 相似文献
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We describe polymerase chain reaction (PCR) primers and amplification conditions for seven microsatellite DNA loci isolated from the red‐spotted newt (Notophthalmus viridescens). Primers were tested on 16 individuals from two populations on the Savannah River Site in Aiken County, South Carolina. We detected six to 10 alleles per locus and an overall observed heterozygosity range of 0.31–0.81. Despite low heterozygosity at two of the seven loci, the high polymorphic information contents (from 0.54 to 0.85) of these markers render them useful for future studies of the behavioural and population ecology of this common salamander. 相似文献