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1.
In 2007, we published the results of a genome-wide screen for ORFs that affect
the frequency of Rad52 foci in yeast. That paper was published within the
constraints of conventional online publishing tools, and it provided only a
glimpse into the actual screen data. New tools in the JCB DataViewer now show
how these data can—and should—be shared.
Complete screen data
https://doi.org/10.1083/jcb.201108095.dv The Rad52 protein has pivotal functions in double strand break repair and homologous recombination. The activity of Rad52 is often monitored by the subnuclear foci that it forms spontaneously in S phase or after DNA damage (Lisby et al., 2001). In mammals, the functions of yeast Rad52 may be divided between human RAD52 and the tumor suppressor BRCA2 (Feng et al., 2011). The full host of molecular players that govern Rad52 focus formation and maintenance was not well known when we initiated our screen. Using a high-content, image-based assay, we assessed the proportion of cells containing spontaneous Rad52-YFP foci in 4,805 viable Saccharomyces cerevisiae deletion strains (Alvaro et al., 2007). Starting with 96-well arrays of a deletion strain library, we created hybrid diploid strains (homozygous for the deletions) using systematic hybrid loss of heterozygosity (SHyLOH; Alvaro et al., 2006). We then manually and sequentially examined each strain using epifluorescence microscopy for the presence of Rad52-YFP foci. All of our image analysis was performed manually.As is often the case, our screen was published showing only a couple of representative images and providing data tables to summarize the findings. Tomes of data that could not be included in the published paper were relegated to supplemental Excel tables, typical of genome-wide screens. Also, the raw image data were sequestered in the laboratory on DVDs. With considerable help from JCB and Glencoe Software, we are delighted that the raw data from our Rad52 screen are now freely available online through the JCB DataViewer. A new interface within the JCB DataViewer brings presentation and preservation of high-content, multidimensional image-based screening data to a whole new level. To facilitate the development of this new interface, JCB required a dataset that was not time sensitive, and we were happy to provide our previously published Rad52 data. In the future, this new interface will be used to present high-content screening (HCS) datasets linked to published JCB papers. Indeed, the first publication of this sort appears in this issue of JCB (Rohn et al., 2011).The presentation of our data in the JCB DataViewer clearly shows the many benefits of this new publishing resource for the scientific community. Users now can view the complete collection of 3D image data across the entire screen, not just the two images in our original publication (Alvaro et al., 2007). Additionally, detailed information on image acquisition parameters, locus identities, and more is easily accessible (Fig. 1). Phenotypic scoring results can be visualized in interactive chart formats (Fig. 1), and search (Fig. 2) and database-linking tools (Fig. 1) allow extensive mining of the data for genes and phenotypes of interest. These tools provide an unprecedented view into HCS data in their entirety, as well as a means for authors to share and archive their data. This kind of accessibility to the direct visualization of the entire set of original screening data, on a scale previously only available to the scientists performing the screen, allows users to understand the full context of the image data analyzed in a screen. Furthermore, it is only through full access to the raw images and associated metadata that this information can be of maximum use to the community for large-scale data mining.Open in a separate windowFigure 1.The HCS interface of the JCB DataViewer provides interactive tools for the analysis of complete datasets from image-based screens. The miniviewer (top left) provides information for each gene in the screen through a zoomable and scrollable display of original multidimensional image data. It contains detailed metadata and a gene ontology (GO) summary, a link to a relevant external database (e.g., the Saccharomyces Genome Database [SGD]; top right), and a link to phenotypic scoring data for the complete screen in the chart view (bottom right). Within the chart view, hits designated by the screen authors are shown in blue, and the strain currently on display in the miniviewer is shown in red. The plate view (bottom left) shows the position of the strain of interest (red box) relative to other strains screened.Open in a separate windowFigure 2.The HCS interface of the JCB DataViewer provides search tools for the mining of complete datasets from image-based screens. (A) Users can search screen data by gene name or keywords (e.g., DNA repair). (B) Users can pick candidates for further analysis from the phenotypic scoring information in the chart view.As in all large-scale screens, the real data are variable; e.g., some strains provide a clear Rad52 focus phenotype, whereas others are more ambiguous. For our particular screen, images were not collected using automated technology but were acquired manually, strain by strain, over a period of months, leading to different levels of fluorescence intensity of Rad52-YFP as a result of, for example, changes in the intensity of our mercury arc lamp. Differences also exist in the number of fields and z stacks captured for each strain. In the absence of automated image collection, images from the primary screen in a few cases were not archived with the others and thus for all intents and purposes have been lost. In addition, our Rad52 screen only assayed nonessential genes, and some mutants are refractory to the SHyLOH methodology. Knowing all of this information allows users to view the data in a realistic manner and further highlights the importance of providing a central repository to archive HCS data.When published through conventional publication media, many important imaging details are known only to the original screeners. The new HCS interface of the JCB DataViewer shines a light on screening data as metadata become freely accessible, allowing any user to ask novel questions of the dataset. For example, the plate view for images (Fig. 1) allows users to assess whether neighboring colonies played any role in determining the phenotype and to delve deeper into why that might be. For example, are any “hits” a result of contamination from adjacent strains, resulting in clusters of positives? In the context of an automated screen, how were control and experimental samples arrayed across a plate during data collection? Did the controls on a particular plate behave as expected? Because our screen used a novel chromosome-specific loss of the heterozygosity method, users can ask whether mutations on specific chromosomes share features of Rad52 foci levels. The global resolution of the dataset provided through this new interface puts users of the dataset as close to the seat of the original screening scientist as possible, allowing them to ask, “what did the authors really see?”Presenting HCS data in the JCB DataViewer holds immense potential value to the scientific community. Through this new interface, users can access powerful interactive tools for analyzing scored phenotypes across the entire dataset (Fig. 1). Each gene ID can be charted against the phenotypic parameters scored in the original screen (e.g., the percentage of cells with Rad52 foci) and compared with all other loci (Fig. 1). Users can take our data and create their own list of hits based on their criteria, create a gallery of thumbnails for their selections (Fig. 2), and seamlessly move between their list of hits and the original data in the plate display format (Fig. 1). Users can also compare their candidates with our list (Fig. 2). The ability to visualize these data for comparative analyses creates a whole new perspective. The HCS interface of the JCB DataViewer allows users to look for their favorite gene, compare related genes, and discover new genes they never anticipated were involved in a given process.In summary, these new features of the JCB DataViewer will allow users to access the primary data from large-scale screens and to look at the full dataset to see what all of the images really look like. The ability to mine these data opens up whole new dimensions in data sharing and transparency. In the future, we anticipate that it will be possible to search many genome-wide screens, such as our Rad52 dataset, to identify commonalities in protein localization, concentration, cell morphology, etc. However, this will only occur if image data are archived and made freely available to the scientific community. We wholeheartedly support the efforts of JCB and hope that groups that use image-based HCS will increasingly make their images available using tools such as the JCB DataViewer. 相似文献2.
3.
FtsZ, a bacterial homolog of eukaryotic tubulin, assembles into the Z ring required for cytokinesis. In Escherichia coli, FtsZ interacts directly with FtsA and ZipA, which tether the Z ring to the membrane. We used three-dimensional structured illumination microscopy to compare the localization patterns of FtsZ, FtsA, and ZipA at high resolution in Escherichia coli cells. We found that FtsZ localizes in patches within a ring structure, similar to the pattern observed in other species, and discovered that FtsA and ZipA mostly colocalize in similar patches. Finally, we observed similar punctate and short polymeric structures of FtsZ distributed throughout the cell after Z rings were disassembled, either as a consequence of normal cytokinesis or upon induction of an endogenous cell division inhibitor.The assembly of the bacterial tubulin FtsZ has been well studied in vitro, but the fine structure of the cytokinetic Z ring it forms in vivo is not well defined. Super-resolution microscopy methods including photoactivated localization microscopy (PALM) and three-dimensional-structured illumination microscopy (3D-SIM) have recently provided a more detailed view of Z-ring structures. Two-dimensional PALM showed that Z rings in Escherichia coli are likely composed of loosely-bundled dynamic protofilaments (1,2). Three-dimensional PALM studies of Caulobacter crescentus initially showed that Z rings were comprised of loosely bundled protofilaments forming a continuous but dynamic ring (1–3). However, a more recent high-throughput study showed that the Z rings of this bacterium are patchy or discontinuous (4), similar to Z rings of Bacillus subtilis and Staphylococcus aureus using 3D-SIM (5). Strauss et al. (5) also demonstrated that the patches in B. subtilis Z rings are highly dynamic.Assembly of the Z ring is modulated by several proteins that interact directly with FtsZ and enhance assembly or disassembly (6). For example, FtsA and ZipA promote ring assembly in E. coli by tethering it to the cytoplasmic membrane (7,8). SulA is an inhibitor of FtsZ assembly, induced only after DNA damage, which sequesters monomers of FtsZ to prevent its assembly into a Z ring (9). Our initial goals were to visualize Z rings in E. coli using 3D-SIM, and then examine whether any FtsZ polymeric structures remain after SulA induction. We also asked whether FtsA and ZipA localized in patchy patterns similar to those of FtsZ.We used a DeltaVision OMX V4 Blaze microscope (Applied Precision, GE Healthcare, Issaquah, WA) to view the high-resolution localization patterns of FtsZ in E. coli cells producing FtsZ-GFP (Fig. 1). Three-dimensional views were reconstructed using softWoRx software (Applied Precision). To rule out GFP artifacts, we also visualized native FtsZ from a wild-type strain (WM1074) by immunofluorescence (IF).Open in a separate windowFigure 1Localization of FtsZ in E. coli. (A) Cell with a Z ring labeled with FtsZ-GFP. (B) Rotated view of Z ring in panel A. (C) Cell with a Z ring labeled with DyLight 550 (Thermo Fisher Scientific, Waltham, MA). (D) Rotated view of Z ring in panel C. (B1 and D1) Three-dimensional surface intensity plots of Z rings in panels B and D, respectively. (E) A dividing cell producing FtsZ-GFP. The cell outline is shown in the schematic. (Asterisk) Focus of FtsZ localization; (open dashed ovals) filamentous structures of FtsZ. Three-dimensional surface intensity plots were created using the software ImageJ (19). Scale bars, 1 μm.Both FtsZ-GFP (Fig. 1, A, B, and B1) and IF staining for FtsZ (Fig. 1, C, D, and D1) consistently localized to patches around the ring circumference, similar to the B. subtilis and C. crescentus FtsZ patterns (4,5). Analysis of fluorescence intensities (see Fig. S1, A and B, in the Supporting Material) revealed that the majority of Z rings contain one or more gaps in which intensity decreases to background levels (82% for FtsZ-GFP and 69% for IF). Most rings had 3–5 areas of lower intensity, but only a small percentage of these areas had fluorescence below background intensity (34% for FtsZ-GFP and 21% for IF), indicating that the majority of areas with lower intensity contain at least some FtsZ.To elucidate how FtsZ transitions from a disassembled ring to a new ring, we imaged a few dividing daughter cells before they were able to form new Z rings (Fig. 1
E). Previous conventional microscopy had revealed dynamic FtsZ helical structures (10), but the resolution had been insufficient to see further details. Here, FtsZ visualized in dividing cells by 3D-SIM localized throughout as a mixture of patches and randomly-oriented short filaments (asterisk and dashed oval in Fig. 1, respectively). These structures may represent oligomeric precursors of Z ring assembly.To visualize FtsZ after Z-ring disassembly another way, we overproduced SulA, a protein that blocks FtsZ assembly. We examined E. coli cells producing FtsZ-GFP after induction of sulA expression from a pBAD33-sulA plasmid (pWM1736) with 0.2% arabinose. After 30 min of sulA induction, Z rings remained intact in most cells (Fig. 2
A and data not shown). The proportion of cellular FtsZ-GFP in the ring before and after induction of sulA was consistent with previous data (data not shown) (1,11).Open in a separate windowFigure 2Localization of FtsZ after overproduction of SulA. (A) Cell producing FtsZ-GFP after 0.2% arabinose induction of SulA for 30 min. (B) After 45 min. (B1) Magnified cell shown in panel B. (C) Cell producing native FtsZ labeled with AlexaFluor 488 (Life Technologies, Carlsbad, CA) 30 min after induction; (D) 45 min after induction. (D1) Magnified cell shown in panel D. Scale bars, 1 μm. (Asterisk) Focus of FtsZ localization; (open dashed ovals) filamentous structures of FtsZ.Notably, after 45 min of sulA induction, Z rings were gone (Fig. 2, B and B1), replaced by numerous patches and randomly-oriented short filaments (asterisk and dashed ovals in Fig. 2), similar to those observed in a dividing cell. FtsZ normally rapidly recycles from free monomers to ring-bound polymers (11), but a critical concentration of SulA reduces the pool of available FtsZ monomers, resulting in breakdown of the Z ring (9). The observed FtsZ-GFP patches and filaments are likely FtsZ polymers that disassemble before they can organize into a ring.We confirmed this result by overproducing SulA in wild-type cells and detecting FtsZ localization by IF (Fig. 2, C, D, and D1). The overall fluorescence patterns in cells producing FtsZ-GFP versus cells producing only native FtsZ were similar (Fig. 2, B1 and D1), although we observed fewer filaments with IF, perhaps because FtsZ-GFP confers slight resistance to SulA, or because the increased amount of FtsZ in FtsZ-GFP producing cells might titrate the SulA more effectively.Additionally, we wanted to observe the localization patterns of the membrane tethers FtsA and ZipA. Inasmuch as both proteins bind to the same C-terminal conserved tail of FtsZ (12–14), they would be expected to colocalize with the circumferential FtsZ patches in the Z ring. We visualized FtsA using protein fusions to mCherry and GFP (data not shown) as well as IF using a wild-type strain (WM1074) (Fig. 3
A). We found that the patchy ring pattern of FtsA localization was similar to the FtsZ pattern. ZipA also displayed a similar patchy localization in WM1074 by IF (Fig. 3
B).Open in a separate windowFigure 3Localization of FtsA (A) and ZipA (B) by IF using AlexaFluor 488. (C) FtsA-GFP ring. (D) Same cell shown in panel C with ZipA labeled with DyLight 550. (C1 and D1) Three-dimensional surface intensity plots of FtsA ring from panel C or ZipA ring from panel D, respectively. (E) Merged image of FtsA (green) and ZipA (red) from the ring shown in panels C and D. (F) Intensity plot of FtsA (green) and ZipA (red) of ring shown in panel E. The plot represents intensity across a line drawn counterclockwise from the top of the ring around the circumference, then into its lumen. Red/green intensity plot and three-dimensional surface intensity plots were created using the software ImageJ (19). Scale bar, 1 μm.To determine whether FtsA and ZipA colocalized to these patches, we used a strain producing FtsA-GFP (WM4679) for IF staining of ZipA using a red secondary antibody. FtsA-GFP (Fig. 3
C) and ZipA (Fig. 3
D) had similar patterns of fluorescence, although the three-dimensional intensity profiles (Fig. 3, C1 and D1) reveal slight differences in intensity that are also visible in a merged image (Fig. 3
E). Quantitation of fluorescence intensities around the circumference of the rings revealed that FtsA and ZipA colocalized almost completely in approximately half of the rings analyzed (Fig. 3
F, and see Fig. S2
A), whereas in the other rings there were significant differences in localization in one or more areas (see Fig. S2
B). FtsA and ZipA bind to the same C-terminal peptide of FtsZ and may compete for binding. Cooperative self-assembly of FtsA or ZipA might result in large-scale differential localization visible by 3D-SIM.In conclusion, our 3D-SIM analysis shows that the patchy localization of FtsZ is conserved in E. coli and suggests that it may be widespread among bacteria. After disassembly of the Z ring either in dividing cells or by excess levels of the cell division inhibitor SulA, FtsZ persisted as patches and short filamentous structures. This is consistent with a highly dynamic population of FtsZ monomers and oligomers outside the ring, originally observed as mobile helices in E. coli by conventional fluorescence microscopy (10) and by photoactivation single-molecule tracking (15). FtsA and ZipA, which bind to the same segment of FtsZ and tether it to the cytoplasmic membrane, usually display a similar localization pattern to FtsZ and each other, although in addition to the differences we detect by 3D-SIM, there are also likely differences that are beyond its ∼100-nm resolution limit in the X,Y plane.As proposed previously (16), gaps between FtsZ patches may be needed to accommodate a switch from a sparse Z ring to a more condensed ring, which would provide force to drive ring constriction (17). If this model is correct, the gaps should close upon ring constriction, although this may be beyond the resolution of 3D-SIM in constricted rings. Another role for patches could be to force molecular crowding of low-abundance septum synthesis proteins such as FtsI, which depend on FtsZ/FtsA/ZipA for their recruitment, into a few mobile supercomplexes.How are FtsZ polymers organized within the Z-ring patches? Recent polarized fluorescence data suggest that FtsZ polymers are oriented both axially and circumferentially within the Z ring in E. coli (18). The seemingly random orientation of the non-ring FtsZ polymeric structures we observe here supports the idea that there is no strong constraint requiring FtsZ oligomers to follow a circumferential path around the cell cylinder. The patches of FtsZ in the unperturbed E. coli Z ring likely represent randomly oriented clusters of FtsZ filaments that are associated with ZipA, FtsA, and essential septum synthesis proteins. New super-resolution microscopy methods should continue to shed light on the in vivo organization of these protein assemblies. 相似文献
4.
Sara Kaliman Christina Jayachandran Florian Rehfeldt Ana-Sun?ana Smith 《Biophysical journal》2014,106(7):L25-L28
It is well established that MDCK II cells grow in circular colonies that densify until contact inhibition takes place. Here, we show that this behavior is only typical for colonies developing on hard substrates and report a new growth phase of MDCK II cells on soft gels. At the onset, the new phase is characterized by small, three-dimensional droplets of cells attached to the substrate. When the contact area between the agglomerate and the substrate becomes sufficiently large, a very dense monolayer nucleates in the center of the colony. This monolayer, surrounded by a belt of three-dimensionally packed cells, has a well-defined structure, independent of time and cluster size, as well as a density that is twice the steady-state density found on hard substrates. To release stress in such dense packing, extrusions of viable cells take place several days after seeding. The extruded cells create second-generation clusters, as evidenced by an archipelago of aggregates found in a vicinity of mother colonies, which points to a mechanically regulated migratory behavior.Studying the growth of cell colonies is an important step in the understanding of processes involving coordinated cell behavior such as tissue development, wound healing, and cancer progression. Apart from extremely challenging in vivo studies, artificial tissue models are proven to be very useful in determining the main physical factors that affect the cooperativity of cells, simply because the conditions of growth can be very well controlled. One of the most established cell types in this field of research is the Madin-Darby canine kidney epithelial cell (MDCK), originating from the kidney distal tube (1). A great advantage of this polarized epithelial cell line is that it retained the ability for contact inhibition (2), which makes it a perfect model system for studies of epithelial morphogenesis.Organization of MDCK cells in colonies have been studied in a number of circumstances. For example, it was shown that in three-dimensional soft Matrigel, MDCK cells form a spherical enclosure of a lumen that is enfolded by one layer of polarized cells with an apical membrane exposed to the lumen side (3). These structures can be altered by introducing the hepatocyte growth factor, which induces the formation of linear tubes (4). However, the best-studied regime of growth is performed on two-dimensional surfaces where MDCK II cells form sheets and exhibit contact inhibition. Consequently, the obtained monolayers are well characterized in context of development (5), mechanical properties (6), and obstructed cell migration (7–9).Surprisingly, in the context of mechanics, several studies of monolayer formation showed that different rigidities of polydimethylsiloxane gels (5) and polyacrylamide (PA) gels (9) do not influence the nature of monolayer formation nor the attainable steady-state density. This is supposedly due to long-range forces between cells transmitted by the underlying elastic substrate (9). These results were found to agree well with earlier works on bovine aortic endothelial cells (10) and vascular smooth muscle cells (11), both reporting a lack of sensitivity of monolayers to substrate elasticity. Yet, these results are in stark contrast with single-cell experiments (12–15) that show a clear response of cell morphology, focal adhesions, and cytoskeleton organization to substrate elasticity. Furthermore, sensitivity to the presence of growth factors that are dependent on the elasticity of the substrate in two (16) and three dimensions (4) makes this result even more astonishing. Therefore, we readdress the issue of sensitivity of tissues to the elasticity of the underlying substrate and show that sufficiently soft gels induce a clearly different tissue organization.We plated MDCK II cells on soft PA gels (Young’s modulus E = 0.6 ± 0.2 kPa), harder PA gels (E = 5, 11, 20, 34 kPa), and glass, all coated with Collagen-I. Gels were prepared following the procedure described in Rehfeldt et al. (17); rigidity and homogeneity of the gels was confirmed by bulk and microrheology (see the Supporting Material for comparison). Seeding of MDCK II cells involved a highly concentrated solution dropped in the middle of a hydrated gel or glass sample. For single-cell experiments, cells were dispersed over the entire dish. Samples were periodically fixed up to Day 12, stained for nuclei and actin, and imaged with an epifluorescence microscope. Details are described in the Supporting Material.On hard substrates and glass it was found previously that the area of small clusters expands exponentially until the movement of the edge cannot keep up with the proliferation in the bulk (5). Consequently, the bulk density increases toward the steady state, whereas the density of the edge remains low. At the same time, the colony size grows subexponentially (5). This is what we denote “the classical regime of growth”. Our experiments support these observations for substrates with E ≥ 5 kPa. Specifically, on glass, colonies start as small clusters of very low density of 700 ± 200 cells/mm2 (Fig. 1, A and B), typically surrounded by a strong actin cable (Fig. 1, B and C). Interestingly, the spreading area of single cells (Fig. 1
A) on glass was found to be significantly larger, i.e., (2.0 ± 0.9) × 10−3 mm2. After Day 4 (corresponding cluster area of 600 ± 100 mm2), the density in the center of the colony reached the steady state with 6,800 ± 500 cells/mm2, whereas the mean density of the edge profile grew to 4,000 ± 500 cells/mm2. This density was retained until Day 12 (cluster area 1800 ± 100 mm2), which is in agreement with previous work (9).Open in a separate windowFigure 1Early phase of cluster growth on hard substrates. (A) Well-spread single cells, and small clusters with a visible actin cable 6 h after seeding. (B) Within one day, clusters densify and merge, making small colonies. (C) Edge of clusters from panel B.In colonies grown on 0.6 kPa gels, however, we encounter a very different growth scenario. The average spreading area of single cells is (0.34 ± 0.3) × 10−3 mm2, which is six times smaller than on glass substrates (Fig. 2
A). Clusters of only few cells show that cells have a preference for cell-cell contacts (a well-established flat contact zone can be seen at the cell-cell interface in Fig. 2
A) rather than for cell-substrate contacts (contact zone is diffusive and the shape of the cells appears curved). The same conclusion emerges from the fact that dropletlike agglomerates, resting on the substrate, form spontaneously (Fig. 2
A), and that attempts to seed one single cluster of 90,000 cells fail, resulting in a number of three-dimensional colonies (Fig. 2
A). When the contact area with the substrate exceeds 4.7 × 10−3 mm2, a monolayer appears in the center of such colonies (Fig. 2
B). The colonies can merge, and if individual colonies are small, the collapse into a single domain is associated with the formation of transient irregular structures (Fig. 2
B). Ultimately, large elliptical colonies (average major/minor axis of e = 1.8 ± 0.6) with a smooth edge are formed (Fig. 2
C), unlike on hard substrates where circular clusters (e = 1.06 ± 0.06) with a ragged edge comprise the characteristic phenotype.Open in a separate windowFigure 2Early phase of cluster growth on soft substrates. (A) Twelve hours after seeding, single cells remain mostly round and small. They are found as individual, or within small, three-dimensional structures (top). The latter nucleate a monolayer in their center (bottom), if the contact area with the substrate exceeds ∼5 × 10−3 mm2. (B) Irregularly-shaped clusters appear due to merging of smaller droplets. A stable monolayer surrounded by a three-dimensional belt of densely packed cells is clearly visible, even in larger structures. (C) All colonies are recorded on Day 4.Irrespective of cluster size, in the new regime of growth, the internal structure is built of two compartments (Fig. 2
B):
- 1.The first is the edge (0.019 ± 0.05-mm wide), a three-dimensional structure of densely packed cells. This belt is a signature of the new regime because on hard substrates the edge is strictly two-dimensional (Fig. 1 C).
- 2.The other is the centrally placed monolayer with a spatially constant density that is very weakly dependent on cluster size and age (Fig. 3). The mean monolayer density is 13,000 ± 2,000 cells/mm2, which is an average over 130 clusters that are up to 12 days old and have a size in the range of 10−3 to 10 mm2, each shown by a data point in Fig. 3. This density is twice the steady-state density of the bulk tissue in the classical regime of growth.Open in a separate windowFigure 3Monolayer densities in colonies grown on 0.6 kPa substrates, as a function of the cluster size and age. Each cluster is represented by a single data point signifying its mean monolayer density. (Black lines) Bulk and (red dashed lines) edge of steady-state densities from monolayers grown on glass substrates. Error bars are omitted for clarity, but are discussed in the Supporting Material.
5.
6.
Angelika B. Amon 《Genetics》2014,198(2):425-426
THE Genetics Society of America Medal is awarded to an individual for outstanding contributions to the field of genetics in the past 15 years. Recipients of the GSA Medal are recognized for elegant and highly meaningful contributions to modern genetics. The 2014 recipient, Angelika B. Amon, has uncovered key principles governing the cell cycle and was the first to demonstrate a connection between the physical completion of anaphase and the initiation of mitotic exit. More recently, her research has focused on the consequences of aneuploidy. GENETICS spoke with Dr. Amon about her approach to science and what is next on the horizon.Open in a separate windowAngelika B. Amon 相似文献
7.
The T-cell actin cytoskeleton mediates adaptive immune system responses to peptide antigens by physically directing the motion and clustering of T-cell receptors (TCRs) on the cell surface. When TCR movement is impeded by externally applied physical barriers, the actin network exhibits transient enrichment near the trapped receptors. The coordinated nature of the actin density fluctuations suggests that they are composed of filamentous actin, but it has not been possible to eliminate de novo polymerization at TCR-associated actin polymerizing factors as an alternative cause. Here, we use a dual-probe cytoskeleton labeling strategy to distinguish between stable and polymerizing pools of actin. Our results suggest that TCR-associated actin consists of a relatively high proportion of the stable cytoskeletal fraction and extends away from the cell membrane into the cell. This implies that actin enrichment at mechanically trapped TCRs results from three-dimensional bunching of the existing filamentous actin network.The T-cell actin cytoskeleton is critical for proper antigen recognition by the mammalian adaptive immune system. During T-cell receptor (TCR) triggering by antigen peptides presented on major histocompatibility proteins (pMHCs) on the surfaces of antigen-presenting cells (APCs), the T-cell actin cytoskeleton adopts a pattern of centrosymmetric retrograde flow (1–3). This simultaneously promotes further TCR triggering (4) and rearranges various T-cell membrane proteins and their APC counterparts into an organized cell-cell interface termed the immunological synapse (IS) (5–7). During this process, TCRs form microclusters that move to the center of the IS in an actin-dependent manner (8,9). When engineered physical barriers interrupt the centripetal motion of TCR clusters, actin flow slows near the pinned microclusters, and the cytoskeletal network transiently accumulates and dissipates at the sites (10,11). The amplitude and duration of the induced cytoskeletal fluctuations are much greater than would be expected for a random distribution of independent objects, indicating that the actin in the local environment is coordinated. Whether this coordination arises from a rearrangement in the existing F-actin network or represents de novo polymerization of the cytoskeleton, as predicted by the association of TCRs with actin polymerizing factors (12), remains unclear. Here, we use a dual-probe cytoskeleton labeling approach that has previously been applied to distinguish between stable and dynamic populations of actin by exploiting the different relative affinities of monomeric actin and actin-binding proteins toward each population (13). This strategy reveals that TCR-associated actin is composed primarily of the stable cytoskeletal fraction and that local enrichment results from three-dimensional bunching of the existing filamentous actin network.Primary T cells from mice transgenic for the AND TCR were triggered using synthetic APCs consisting of supported lipid bilayers functionalized with pMHC and the integrin ligand intercellular adhesion molecule 1. Nanopatterned metal grids on the bilayer substrate acted as diffusion barriers that prevented lateral transport of TCR-pMHC complexes (14,15). Transient enrichment of actin at TCR clusters trapped at these barriers was visualized using fluorescent fusions of actin itself (mKate2-β-actin) and the F-actin binding domain of utrophin (EGFP-UtrCH). Such a dual-probe strategy theoretically allows for discrimination between different pools of actin: dynamic populations characterized by high polymerization and/or short filament fragments tend to be relatively better labeled by direct actin fusions whereas stable populations composed of longer filaments can support higher labeling by fluorescent fusions of F-actin binding proteins. This visualization method has been validated in Xenopus oocytes, where it distinguishes actin populations during wound healing (13). It has not been explicitly applied to T cells; however, simultaneous labeling of the Jurkat cell cytoskeleton using EGFP-actin and Alexa 568-phalloidin reveals distinct populations of actin consistent with the results expected from Xenopus (13,16).Our results show that the T-cell periphery is relatively enriched in mKate2-β-actin (Fig. 1
C, box 1), while EGFP-UtrCH dominates toward the center of the IS (Fig. 1
C, box 2). We infer from this probe distribution that the cytoskeleton at the T-cell periphery is composed of short fragments and is a site of active polymerization, whereas at the center of the IS, actin filaments are longer and predominantly stable. This is consistent with previous models of the T-cell actin network (3,16). An effective way to highlight each of these cytoskeletal regions is to consider the relative ratios of the two probes at each location. In this case, a high UtrCH/actin ratio corresponds to stable actin, and a high actin/UtrCH ratio corresponds to dynamic actin (Fig. 1
D). When T cells are treated with cytochalasin D, an inhibitor of actin polymerization, the overall UtrCH/actin ratio of the cell decreases as would be expected from a general decrease in polymerized actin (see Movie S7 and Movie S8 in the Supporting Material). However, it should be noted that photobleaching can also shift the UtrCH/actin ratio over time. We limit quantitative analysis of the ratio to its spatial gradients at a single time point, but such analysis is possible in systems that permit rigorous calibration for probe expression and photobleaching.Open in a separate windowFigure 1Ratiometric imaging of the cytoskeleton in live T cells distinguishes between dynamic and stable actin populations. (A) mKate2-β-actin, (B) EGFP-UtrCH, and (C) merged images of a triggered T cell show different actin pools. The cutouts in panel C correspond to (1) a region high in dynamic actin featuring short, polymerizing filaments and/or actin monomers and (2) a region with a stable actin population featuring longer filaments to which UtrCH can bind. (D) The UtrCH/actin ratio image highlights pools of relatively high UtrCH (red) or actin (blue). (Scale bars: 5 μm.)Actin enrichment at trapped TCR clusters incorporates both mKate2-β-actin (Fig. 2, A and C) and EGFP-UtrCH (Fig. 2, B and C). The relative UtrCH/actin ratio at these sites (Fig. 2
D, box 2) is quite high relative to nearby background areas (Fig. 2
D, box 1), indicating that the actin is derived primarily from the stable actin population.Open in a separate windowFigure 2Receptor-induced cytoskeletal enrichment at sites of pinned TCRs corresponds to a primarily stable actin fraction. (A) mKate2-β-actin, (B) EGFP-UtrCH, and (C) merged images of a triggered T cell interacting with a nanopatterned supported lipid bilayer show actin enrichment corresponding to putative sites of pinned TCRs. (D) The UtrCH/actin ratio is high at sites displaying actin enrichment, indicating a primarily stable actin fraction in (1) these regions compared to (2) nearby background areas. (Scale bars: 5 μm.)The three-dimensional distribution of TCR-associated actin was analyzed in dual-labeled live T cells using a spinning disk confocal microscope. The recordings show actin extending away from the cell membrane in the vicinity of trapped TCRs, while the rest of the actin cytoskeleton remains relatively flat (Fig. 3 and see Fig. S1 in the Supporting Material). These protrusions of actin away from the membrane surface are predominantly composed of stable, filamentous actin, as indicated by their relatively high UtrCH/actin ratio (Fig. 3
B).Open in a separate windowFigure 3Three-dimensional ratiometric imaging shows that actin enrichment extends away from the cell membrane. Single planes from (A) merged mKate2-β-actin and EGFP-UtrCH and (B) UtrCH/actin ratio three-dimensional stacks show actin enrichment at the cell membrane. Cutouts represent Z projections passing through sites of (1) enrichment and (2) nearby background regions. The color distribution in panel B is analogous to that in Figs. 1D and and22D, and is omitted for clarity. (Scale bar: 5 μm in the x axis only. Scale box: 1 μm.)Our interpretation of these results is that the filamentous actin network is relatively dense at sites of pinned TCRs. This is the simplest explanation out of several possibilities, one of which is formin-mediated mKate2-β-actin-deficient actin nucleation (17). Filament bunching at pinned TCRs can arise from consistent biophysical properties without assuming heterogeneity between the biochemistry of these receptors and other actin-associated proteins such as those at the cell edge, where locally high probe ratios are absent.Although TCRs are intentionally trapped as part of this experimental strategy, it is likely APCs can naturally impede TCR ligand mobilities under certain circumstances, and this has been shown to impact T-cell signaling (18,19). Actin architecture near cell surface proteins has been extensively studied in focal adhesions of fibroblasts (20), but the lack of stress fibers in T cells makes it unlikely that the two structures are similar. Thus, receptor-induced cytoskeletal enrichment at TCR clusters adds to the catalog of actin behaviors in situ, which is conveniently probed by techniques such as ratiometric dual-probe imaging in live cells. These techniques can be coupled to various spatial analysis algorithms to further extend their utility. 相似文献
8.
Magnus Andersson Jakob?P. Ulmschneider Martin?B. Ulmschneider Stephen?H. White 《Biophysical journal》2013,104(6):L12-L14
The distribution of peptide conformations in the membrane interface is central to partitioning energetics. Molecular-dynamics simulations enable characterization of in-membrane structural dynamics. Here, we describe melittin partitioning into dioleoylphosphatidylcholine lipids using CHARMM and OPLS force fields. Although the OPLS simulation failed to reproduce experimental results, the CHARMM simulation reported was consistent with experiments. The CHARMM simulation showed melittin to be represented by a narrow distribution of folding states in the membrane interface.Unstructured peptides fold into the membrane interface because partitioned hydrogen-bonded peptide bonds are energetically favorable compared to free peptide bonds (1–3). This folding process is central to the mechanisms of antimicrobial and cell-penetrating peptides, as well as to lipid interactions and stabilities of larger membrane proteins (4). The energetics of peptide partitioning into membrane interfaces can be described by a thermodynamic cycle (Fig. 1). State A is a theoretical state representing the fully unfolded peptide in water, B is the unfolded peptide in the membrane interface, C is the peptide in water, and D is the folded peptide in the membrane. The population of peptides in solution (State C) is best described as an ensemble of folded and unfolded conformations, whereas the population of peptides in State D generally is assumed to have a single, well-defined helicity, as shown in Fig. 1
A (5). Given that, in principle, folding in solution and in the membrane interface should follow the same basic rules, peptides in state D could reasonably be assumed to also be an ensemble. A fundamental question (5) is therefore whether peptides in state D can be correctly described as having a single helicity. Because differentiating an ensemble of conformations and a single conformation may be an impossible experimental task (5), molecular-dynamics (MD) simulations provide a unique high-resolution view of the phenomenon.Open in a separate windowFigure 1Thermodynamic cycles for peptide partitioning into a membrane interface. States A and B correspond to the fully unfolded peptide in solution and membrane interface, respectively. The folded peptide in solution is best described as an ensemble of unfolded and folded conformations (State C). State D is generally assumed to be one of peptides with a narrow range of conformations, but the state could actually be an ensemble of states as in the case of State C.Melittin is a 26-residue, amphipathic peptide that partitions strongly into membrane interfaces and therefore has become a model system for describing folding energetics (3,6–8). Here, we describe the structural dynamics of melittin in a dioleoylphosphatidylcholine (DOPC) bilayer by means of two extensive MD simulations using two different force fields.We extended a 12-ns equilibrated melittin-DOPC system (9) by 17 μs using the Anton specialized hardware (10) with the CHARMM22/36 protein/lipid force field and CMAP correction (11,12) (see Fig. S1 and Fig. S2 in the Supporting Material). To explore force-field effects, a similar system was simulated for 2 μs using the OPLS force field (13) (see Methods in the Supporting Material). In agreement with x-ray diffraction measurements on melittin in DOPC multilayers (14), melittin partitioned spontaneously into the lipid headgroups at a position below the phosphate groups at similar depth as glycerol/carbonyl groups (Fig. 2).Open in a separate windowFigure 2Melittin partitioned into the polar headgroup region of the lipid bilayer. (A) Snapshot of the simulation cell showing two melittin molecules (MLT1 and MLT2, in yellow) at the lipid-water interface. (B) Density cross-section of the simulation cell extracted from the 17-μs simulation. The peptides are typically located below the lipid phosphate (PO4) groups, in a similar depth as the glycerol/carbonyl (G/C) groups.To describe the secondary structure for each residue, we defined helicity by backbone dihedral angles (φ, ψ) within 30° from the ideal α-helical values (–57°, –47°). The per-residue helicity in the CHARMM simulation displays excellent agreement with amide exchange rates from NMR measurements that show a proline residue to separate two helical segments, which are unfolded below Ala5 and above Arg22 (15) (Fig. 3
A). In contrast, the OPLS simulation failed to reproduce the per-residue helicity except for a short central segment (see Fig. S3).Open in a separate windowFigure 3Helicity and conformational distribution of melittin as determined via MD simulation. (A) Helicity per residue for MLT1 and MLT2. (B) Corresponding evolution of the helicity. (C) Conformational distributions over the entire 17-μs simulation.Circular dichroism experiments typically report an average helicity of ∼70% for melittin at membrane interfaces (3,6,16,17), but other methods yield average helicities as high as 85% (15,18). Our CHARMM simulations are generally consistent with the experimental results, especially amide-exchange measurements (15); melittin helicity averaged to 78% for MLT1, whereas MLT2 transitioned from 75% to 89% helicity at t ≈ 8 μs, with an overall average helicity of 82% (Fig. 3
B). However, in the OPLS simulation, melittin steadily unfolds over the first 1.3 μs, after which the peptide remains only partly folded, with an average helicity of 33% (see Fig. S3). Similar force-field-related differences in peptide helicity were recently reported, albeit at shorter timescales (19). Although suitable NMR data are not presently available, we have computed NMR quadrupolar splittings for future reference (see Fig. S4).To answer the question asked in this article—whether the conformational space of folded melittin in the membrane interface can be described by a narrow distribution—the helicity distributions for the equilibrated trajectories are shown in Fig. 3
C. Whereas MLT1 in the CHARMM simulation produces a single, narrow distribution of the helicity, MLT2 has a bimodal distribution as a consequence of the folding event at t ≈ 8 μs (Fig. 3
C). We note that CHARMM force fields have a propensity for helix-formation and this transition might therefore be an artifact. We performed a cluster analysis to describe the structure of the peptide in the membrane interface. The four most populated conformations in the CHARMM simulation are shown in Fig. 4. The dominant conformation for both peptides was a helix kinked at G12 and unfolded at the last 5–6 residues of the C-terminus. The folding transition of MLT2 into a complete helix is visible by the 48% occupancy of a fully folded helix.Open in a separate windowFigure 4Conformational clusters of the two melittin peptides (MLT1 and MLT2) from the 17-μs CHARMM simulation in DOPC. Clustering is based on Cα-RMSD with a cutoff criterion of 2 Å.We conclude that the general assumption when calculating folding energetics holds: Folded melittin partitioned into membrane interfaces can be described by a narrow distribution of conformations. Furthermore, extended (several microsecond) simulations are needed to differentiate force-field effects. Although the CHARMM and OPLS simulations would seem to agree for the first few hundred nanoseconds, the structural conclusions differ drastically with longer trajectories, with CHARMM parameters being more consistent with experiments. However, as implied by the difference in substate distributions between MLT1 and MLT2, 17 μs might not be sufficient to observe the fully equilibrated partitioning process. The abrupt change in MLT2 might indicate that the helicity will increase to greater than experimentally observed in a sufficiently long simulation. On the other hand, it could be nothing more than a transient fluctuation. Increased sampling will provide further indicators of convergence of the helix partitioning process. 相似文献
9.
Andrew?K. Lewis Zachary?M. James Jesse?E. McCaffrey Anthony?R. Braun Christine?B. Karim David?D. Thomas Jonathan?N. Sachs 《Biophysical journal》2014,106(6):L21-L24
It has long been presumed that activation of the apoptosis-initiating Death Receptor 5, as well as other structurally homologous members of the TNF-receptor superfamily, relies on ligand-stabilized trimerization of noninteracting receptor monomers. We and others have proposed an alternate model in which the TNF-receptor dimer—sitting at the vertices of a large supramolecular receptor network of ligand-bound receptor trimers—undergoes a closed-to-open transition, propagated through a scissorslike conformational change in a tightly bundled transmembrane (TM) domain dimer. Here we have combined electron paramagnetic resonance spectroscopy and potential-of-mean force calculations on the isolated TM domain of the long isoform of DR5. The experiments and calculations both independently validate that the opening transition is intrinsic to the physical character of the TM domain dimer, with a significant energy barrier separating the open and closed states.Death receptor 5 (DR5) is a member of the tumor necrosis factor receptor (TNFR) superfamily that mediates apoptosis when bound by its cognate ligand, TNF-related apoptosis-inducing ligand (1). Upregulated in cancer cells, DR5 is among the most actively pursued anticancer targets (2). TNF-related apoptosis-inducing ligand binds to preassembled DR5 trimers at their extracellular domains, causing the formation of oligomeric ligand-receptor networks that are held together by receptor dimers (3). In the long-isoform of DR5, this dimer is crosslinked via ligand-induced disulfide bond formation between two transmembrane (TM) domain α-helices at Cys-209, and is further stabilized by a GxxxG motif one helix-turn downstream (3).Our recent study of the structurally homologous TNFR1 showed that receptor activation involves a conformational change that propagates from the extracellular domain to the cytosolic domain through a separation (or opening) of the TM domains of the dimer (4). We have therefore hypothesized that the activation of DR5, and indeed all structurally homologous TNF-receptors, involves a scissorslike opening of the TM domain dimer (Fig. 1).Open in a separate windowFigure 1Activation model of the DR5-L TM dimer. The sequence and positions of the disulfide bond and TOAC spin label (top), along with our previously published model (bottom, left) are shown. We propose an activation model (bottom, right) in which the transmembrane dimer pivots at its disulfide bond to reach an active open conformation.Using electron paramagnetic resonance (EPR) spectroscopy, a technique that has been used previously to study TM helix architecture and dynamics (5,6), and potential-of-mean force (PMF) calculations (7,8), this study addresses the question of whether the isolated disulfide-linked DR5-L TM domain dimer occupies distinct open and closed states (Fig. 1), and how its dynamic behavior contributes to the free-energy landscape of the opening transition of the full-length receptor.The DR5-L TM domain was synthesized with TOAC, an amino acid with a nitroxide spin label rigidly fixed to the α-carbon (9), incorporated at position 32 (Fig. 1), with some minor modification to facilitate EPR measurements. Previous work confirmed that this peptide forms disulfide-linked dimers (e.g., via comparison to 2-ME treated sample) and a negligible population of higher-order oligomers (further supported by model fitting of the EPR data below). For peptide work, residues were renumbered such that Thr-204 corresponds to Thr-1, and so on. The cytosolic Cys-29 (which we previously showed does not participate in a disulfide bond in cells) was replaced with serine to prevent the formation of antiparallel disulfide-linked dimers, and Trp-34 was replaced with tyrosine to prevent intrinsic fluorescence in fluorescence studies (not published). Continuous-wave (CW) dipolar EPR (sensitive only to spin-spin distances <25 Å) was used to measure TOAC-TOAC distances within the TM dimers and revealed an ordered Gaussian distribution centered at 16 Å (full width half-maximum (FWHM) = 4 Å), corresponding to a closed state (Fig. 2
A). Double electron-electron resonance (DEER) (sensitive to spin-spin distances from 15 to 60 Å) also detected a short distance consistent with the dipolar EPR data, along with a longer, disordered component (32.9 Å, FWHM = 28 Å) (Fig. 2
B). Together, these measurements indicate the presence of a compact, ordered closed state and a broader, disordered open state. EPR on oriented membranes also indicated two structural states. Global fitting revealed two populations of spin-label tilt angles (orientation of the nitroxide principal axis relative to the membrane normal): a narrow conformation (24°, FWHM = 20°), and a disordered conformation (50°, FWHM = 48°) (Fig. 2
C). This bimodal orientational distribution (Fig. 2
C) is remarkably consistent with the bimodal distance distribution (Fig. 2
B).Open in a separate windowFigure 2EPR spectra (left) of 32-TOAC-DR5 in lipid, and resulting structural distributions (right). (A) CW dipolar EPR spectra (left) of dimer (1 mM diamide) and monomer (1 mM 2-mercaptoethanol). Best-fit spin-spin distance distribution was a single Gaussian centered at 16 ± 2 Å (right). (B) The DEER waveform (left) of 32-TOAC-DR5 dimer was best fit (right) to a two-Gaussian distribution. The short distance was constrained to agree with the CW data, because DEER has poor sensitivity for distances <20 Å. The long-distance distribution is centered at 32.9 Å and is much broader. (C) CW EPR spectra (left) of 32-TOAC-DR5, with the membrane-normal oriented parallel (red) and perpendicular (blue) to the field. Simultaneous (global) fitting of these spectra reveals narrow and broad components (right). (In panels B and C, the overall distribution is plotted as black, while the closed and open components are plotted as green and magenta, respectively.)We subsequently conducted a PMF calculation (10) using the DR5-L TM dimer starting configuration developed by our group previously (3), embedded in a DMPC bilayer, with the Leu-32/Leu-32 Cα distance as the reaction coordinate. Three calculations were run from independent starting configurations, each using 50 windows spaced in 0.5° increments, and run for 20 ns at each window (totaling 3 μs). Each of the calculations yielded a similar result, and the averaged free energy curve (Fig. 3
A) agrees remarkably well with our EPR measurements: a narrow distribution at the closed conformation (∼16 Å, Fig. 3
B) separated by an ∼3 kcal/mol energy barrier from a broad distribution of accessible open conformations at ∼27 Å, (Fig. 3
C). Each of the three individual PMF plots can be found in Fig. S1 in the Supporting Material.Open in a separate windowFigure 3(A) PMF calculation of the DR5 TM domain dimer along the Leu-32/Leu-32 distance reaction coordinate. The PMF calculation reveals a narrow closed state and a broader open state separated by a free energy barrier. Representative snapshots of the (B) closed state and (C) open state.In the closed state, the helices are tightly packed at the GxxxG interfacial motif and all the way down the juxtaposed helix faces at residues Ala-18, Leu-22, Ala-25, and Val-26. The tight packing is aided by kinking and twisting of the two helices around their common axis, increasing the interacting surface area. In the open conformations, the Ala-18, Leu-22, Ala-25, and Val-26 pairs are dissociated and, interestingly, the GxxxG motif at Gly-10 and Gly-14 remains tightly packed. The open state energy well is only slightly less favorable than the closed state (by ∼2 kcal/mol), and its free energy profile is relatively broad and flat. The increased crossing angle in the open state is facilitated by straightening of the helix kink and is not accommodated by a change in bilayer thickness (see Fig. S3, A and B).The observed change in helix-helix distance (11 Å between the two minima in the PMF) is extremely close to that observed previously in live-cell FRET studies of a constitutively active form of TNFR1 (∼8 Å change between states using large fluorescence probes at the cytosolic domains) (4). The change observed in the EPR data (17 Å) may be an overestimate because the measurement is made between TOAC spin labels that likely protrude from the two helices, depending on rotational orientation. These results collectively show that activation of these receptors requires a small, but clearly significant conformational opening of the TM domains. One important note is that our EPR experiments recapitulate the equilibrium distribution of the two states despite there being no driving force to traverse the barrier between them (∼3 kcal/mol in the closed-to-open transition and ∼1 kcal/mol in the open-to-closed transition, Fig. 3). We do not interpret the results to mean that the dimer necessarily traverses these barriers at 4°C. Rather, there likely exist multiple reaction paths for dimerization of the abstracted TM domains. Finally, in the context of the full-length receptor, how the ligand induces a conformational change capable of overcoming the closed-to-open barrier remains an important question.Whether the observed structural transition in the TM domain dimer of the long-isoform of DR5 is a ubiquitous conformational switch that acts over the entire TNFR superfamily remains unknown. Vilar et al. (11) first proposed a similar scissors-model for activation of p75 neurotrophin receptor, which has a cysteine at the center of its TM helix. The short isoform of DR5 lacks a TM domain cysteine, but does form noncovalent dimers in cells, with likely TM domain dimer contacts (3). Among the other closely related and structurally homologous members of the TNFR superfamily, TNFR1 contains a cysteine at the center of the TM domain, but lacks any discernible small residue motifs (e.g., GxxxG). TNFR2 lacks a TM cysteine on the extracellular side, but does have a GxxxG motif positioned similarly to that of DR5. On the other hand, Death Receptor 4, whose functional distinction from DR5 has remained somewhat elusive, lacks both a cysteine and any recognizable small-residue hydrophobic motif.In summary, we have extended recent findings that point to the TM domain of DR5 as an essential structural component in the conformational change associated with activation. Our findings that the DR5-L TM domain occupies distinct open and closed states, separated by a substantial energy barrier, points the way to further studies across the TNF-receptor superfamily. 相似文献
10.
Joseph Wong David Baddeley Eric?A. Bushong Zeyun Yu Mark?H. Ellisman Masahiko Hoshijima Christian Soeller 《Biophysical journal》2013,104(11):L22-L24
We conducted super-resolution light microscopy (LM) imaging of the distribution of ryanodine receptors (RyRs) and caveolin-3 (CAV3) in mouse ventricular myocytes. Quantitative analysis of data at the surface sarcolemma showed that 4.8% of RyR labeling colocalized with CAV3 whereas 3.5% of CAV3 was in areas with RyR labeling. These values increased to 9.2 and 9.0%, respectively, in the interior of myocytes where CAV3 was widely expressed in the t-system but reduced in regions associated with junctional couplings. Electron microscopic (EM) tomography independently showed only few couplings with caveolae and little evidence for caveolar shapes on the t-system. Unexpectedly, both super-resolution LM and three-dimensional EM data (including serial block-face scanning EM) revealed significant increases in local t-system diameters in many regions associated with junctions. We suggest that this regional specialization helps reduce ionic accumulation and depletion in t-system lumen during excitation-contraction coupling to ensure effective local Ca2+ release. Our data demonstrate that super-resolution LM and volume EM techniques complementarily enhance information on subcellular structure at the nanoscale.The contraction of cardiac ventricular myocytes depends on the rapid cell-wide transient increase in intracellular [Ca2+] upon depolarization of the cell-membrane potential. The cardiac ryanodine receptor (RyR) (1), which is the intracellular Ca2+ release channel in the sarcoplasmic reticulum (SR), plays a central role in shaping Ca2+ transients. RyRs form clusters of various sizes (2,3) with the majority located within junctions between the SR and the surface membrane and its cytoplasmic extension, the transverse tubular (t-) system. It has been suggested that some RyR clusters are associated with caveolae, a specialized signaling microdomain of the surface membrane. Previous studies were complicated by the limited resolution of optical imaging methods of ∼250 nm, much larger than the nanometer scale of RyRs and caveolae. Accordingly, these studies report varying colocalization between RyRs and caveolin-3 (CAV3), a caveolar marker also expressed in the t-system (4,5).In this work, we investigated the relative distribution of CAV3 and RyRs in mouse ventricular myocytes both in the cytosol and near the cell surface with super-resolution fluorescence microscopy that achieves a resolution approaching 30 nm. Our data revealed unexpected local t-system swellings near junctional couplings, which was supported by two different three-dimensional electron microscopy (EM) modalities with <10-nm resolution: EM tomography and serial block-face scanning EM (SBFSEM).Super-resolution images of CAV3 and RyR labeling at the surface sarcolemma of mouse myocytes showed little overlap, suggesting that few RyRs were in couplings with caveolae (Fig. 1
A, for detailed methods, see the Supporting Material). Only ∼4.8% of RyR labeling was associated with CAV3 positive areas and ∼3.5% of CAV3 associated with RyR positive areas (n = 6 cells from three animals, Fig. 1
B, see also Table S1 in the Supporting Material), broadly consistent with previous data in rats (6). To support this finding, EM tomography was applied to mouse ventricular tissue that included a part of the surface sarcolemma, to our knowledge for the first time. Segmentation of peripheral couplings (containing RyR foot structures) and surface caveolae (∼60 nm in diameter and often interconnected) confirmed that the great majority of peripheral couplings were in regions devoid of caveolae (Fig. 1
C). A few junctional couplings containing feet were between caveolae and subsarcolemmal SR (Fig. 1
D, see also Fig. S1 and Movie S1 in the Supporting Material). We conducted a similar analysis in the cytosol where CAV3 expression occurs in the t-system (5) and RyRs are abundant in dyadic junctions between the t-system and SR terminal cisterns.Open in a separate windowFigure 1Colocalization of CAV3 and RyRs at the surface sarcolemma. (A) Super-resolution micrograph of the distribution of CAV3 (green) and RyRs (red) at the surface of a mouse cardiac myocyte. (B) Analysis of the association of CAV3 with RyRs. The fraction of RyR labeling within CAV3 positive areas was ∼4.8% (front data) whereas ∼3.5% of CAV3 was found in RyR-positive membrane areas. (C) Segmented EM tomogram containing a patch of surface sarcolemma (light blue) and associated caveolae (green) as well as peripheral couplings (red). (D) Detailed view of a region with abundant caveolae. (Arrows) Couplings with caveolae.As shown in Fig. 2
A, the spatial distribution of CAV3 and RyR clusters in super-resolution micrographs taken several microns below the surface sarcolemma is consistent with this view. The association of the two labels is slightly increased (as compared to the surface), according to distance analysis with 9% of CAV3 and 9.2% of RyR labeling associating with each other (Fig. 2
B, n = 6 cells from three animals). The similarity of manually traced t-system in EM tomograms (Fig. 2
C) and super-resolved CAV3 labeling suggested that CAV3 is widely distributed in the t-system except for regions where dyadic membrane junctions occur as CAV3 labeling was much weaker in regions with strong RyR labeling. It was notable that the t-system diameter appeared to increase at regions of strong RyR labeling (Fig. 2
D), broadly consistent with the behavior seen in tomograms (Fig. 2
C). This was confirmed by a quantitative analysis of t-tubule diameters in dyadic versus extradyadic regions on the basis of CAV3 and RyR labeling, with full-width at quarter-maximum mean diameters increasing from ∼150 nm distal to dyads, to ∼190 nm (using CAV3 signal only) or ∼280 nm (using CAV3 and RyR signal) near dyads (Fig. 2, G and H, see also Methods in the Supporting Material). The combined RyR and CAV3 signals seemed to be a better representation of the entire t-system lumen near junctions (see Fig. S2).Open in a separate windowFigure 2Distribution of CAV3 and RyRs in the cell interior. (A) Super-resolution micrograph of CAV3 (green) and RyR (red) distribution at t-system. (Arrow) Direction of longitudinal cell axis. (B) Distance analysis of the CAV3 and RyR association (N = 6 cells per group). (C) Segmented EM tomogram of a similar region with three-dimensional mesh models of t-system membrane (green) and dyadic couplings (red). (D) This image illustrates the tracing (white path) of t-tubules. The label distribution was extracted and linearized along the path (E) to calculate a mask that shows the full width at quarter-maximum diameter along tubules, CAV3 (green) and RyR (red) (F). (G) Histograms of local diameters extracted from traced t-tubules. (H) Mean diameters in junctional (dyad) and nonjunctional (ex-dyad) regions. See main text and the Supporting Material for details. **p < 0.01.Taken together, super-resolution imaging and EM tomography strongly support the presence of local t-system dilations in regions where the t-system opposes SR at dyads and such t-system bulges are connected by narrower tubule segments. Further support was provided by SBFSEM, another volume EM technique to study larger cell volumes (albeit at the expense of a slightly lower resolution). SBFSEM clearly showed local t-system dilations were regularly involved in the architecture of most (but not all) dyads (Fig. 3, see also Fig. S3 and Movie S2), as also observed in full three-dimensional super-resolution images (see Fig. S3
C).Open in a separate windowFigure 3Segmented SBFSEM data showing t-system dilations near dyadic junctions. (A) The overview shows t-system membranes (green) and jSR (red) in a mouse myocyte. (B, enlarged inset from panel A) Thin connecting tubules (arrows) and regular swellings in junctional regions at z-lines.Our data identify local dilations of the t-system associated with dyads in mouse cardiac myocytes. Frequent tubule distensions had been observed especially at the intersections of transverse and axial tubules (7), and constrictions were seen in rabbit myocytes although their relationship to dyads was unknown (8). The increased local t-system lumen near junctions may help reduce the predicted ionic accumulation/depletion during excitation-contraction coupling (9). Alternatively, it might simply be secondary to increasing local membrane area and allow the formation of large area junctions that harbor many RyRs. In connection with this point, it would be interesting to investigate the t-system near junctions in species that have larger average tubule diameters (e.g., human and rabbit (10)), or if this architecture changes in mouse heart failure models where t-tubule diameters are often increased.Most peripheral couplings were in regions void of surface caveolae, although a small number of RyR clusters were in junctional couplings between subsarcolemmal SR and caveolae as shown both by the low colocalization between CAV3 and RyRs as well as direct evidence from EM tomography. Similarly, a relatively small fraction of CAV3 colocalized with RyR clusters in the t-system although CAV3 was expressed widely in the t-system. A structural role of CAV3 in the t-system is still unclear—t-tubules in tomogram data did not reveal distinct caveolae shapes on the t-system membrane (see Fig. S4), although this might change in pathology (11). In any case, the t-system exhibits high curvature orthogonal to the tubule axis, which may be supported by CAV3 oligomerization. In addition, the presence of CAV3 in the t-system may be important for regulating other signaling systems (e.g., adrenergic signaling).Finally, our data demonstrate that complementary data from optical super-resolution and three-dimensional EM images assists data interpretation and reliability. We suggest that truly correlative optical and EM imaging approaches should provide further information and improve our knowledge of the basis of cardiac excitation-contraction coupling. 相似文献
11.
12.
Fachuang Lu Jane M. Marita Catherine Lapierre Lise Jouanin Kris Morreel Wout Boerjan John Ralph 《Plant physiology》2010,153(2):569-579
Caffeic acid O-methyltransferase (COMT) is a bifunctional enzyme that methylates the 5- and 3-hydroxyl positions on the aromatic ring of monolignol precursors, with a preference for 5-hydroxyconiferaldehyde, on the way to producing sinapyl alcohol. Lignins in COMT-deficient plants contain benzodioxane substructures due to the incorporation of 5-hydroxyconiferyl alcohol (5-OH-CA), as a monomer, into the lignin polymer. The derivatization followed by reductive cleavage method can be used to detect and determine benzodioxane structures because of their total survival under this degradation method. Moreover, partial sequencing information for 5-OH-CA incorporation into lignin can be derived from detection or isolation and structural analysis of the resulting benzodioxane products. Results from a modified derivatization followed by reductive cleavage analysis of COMT-deficient lignins provide evidence that 5-OH-CA cross couples (at its β-position) with syringyl and guaiacyl units (at their O-4-positions) in the growing lignin polymer and then either coniferyl or sinapyl alcohol, or another 5-hydroxyconiferyl monomer, adds to the resulting 5-hydroxyguaiacyl terminus, producing the benzodioxane. This new terminus may also become etherified by coupling with further monolignols, incorporating the 5-OH-CA integrally into the lignin structure.Lignins are polymeric aromatic constituents of plant cell walls, constituting about 15% to 35% of the dry mass (Freudenberg and Neish, 1968; Adler, 1977). Unlike other natural polymers such as cellulose or proteins, which have labile linkages (glycosides and peptides) between their building units, lignins’ building units are combinatorially linked with strong ether and carbon-carbon bonds (Sarkanen and Ludwig, 1971; Harkin, 1973). It is difficult to completely degrade lignins. Lignins are traditionally considered to be dehydrogenative polymers derived from three monolignols, p-coumaryl alcohol 1h (which is typically minor), coniferyl alcohol 1g, and sinapyl alcohol 1s (Fig. 1; Sarkanen, 1971). They can vary greatly in their composition in terms of their plant and tissue origins (Campbell and Sederoff, 1996). This variability is probably determined and regulated by different activities and substrate specificities of the monolignol biosynthetic enzymes from different sources, and by the carefully controlled supply of monomers to the lignifying zone (Sederoff and Chang, 1991).Open in a separate windowFigure 1.The monolignols 1, and marker compounds 2 to 4 resulting from incorporation of novel monomer 15h into lignins: thioacidolysis monomeric marker 2, dimers 3, and DFRC dimeric markers 4.Recently there has been considerable interest in genetic modification of lignins with the goal of improving the utilization of lignocellulosics in various agricultural and industrial processes (Baucher et al., 2003; Boerjan et al., 2003a, 2003b). Studies on mutant and transgenic plants with altered monolignol biosynthesis have suggested that plants have a high level of metabolic plasticity in the formation of their lignins (Sederoff et al., 1999; Ralph et al., 2004). Lignins in angiosperm plants with depressed caffeic acid O-methyltransferase (COMT) were found to derive from significant amounts of 5-hydroxyconiferyl alcohol (5-OH-CA) monomers 15h (Fig. 1) substituting for the traditional monomer, sinapyl alcohol 1s (Marita et al., 2001; Ralph et al., 2001a, 2001b; Jouanin et al., 2004; Morreel et al., 2004b). NMR analysis of a ligqnin from COMT-deficient poplar (Populus spp.) has revealed that novel benzodioxane structures are formed through β-O-4 coupling of a monolignol with 5-hydroxyguaiacyl units (resulting from coupling of 5-OH-CA), followed by internal trapping of the resultant quinone methide by the phenolic 5-hydroxyl (Ralph et al., 2001a). When the lignin was subjected to thioacidolysis, a novel 5-hydroxyguaiacyl monomer 2 (Fig. 1) was found in addition to the normal guaiacyl and syringyl thioacidolysis monomers (Jouanin et al., 2000). Also, a new compound 3g (Fig. 1) was found in the dimeric products from thioacidolysis followed by Raney nickel desulfurization (Lapierre et al., 2001; Goujon et al., 2003).Further study with the lignin using the derivatization followed by reductive cleavage (DFRC) method also confirmed the existence of benzodioxane structures, with compounds 4 (Fig. 1) being identified following synthesis of the authentic parent compounds 9 (Fig. 2). However, no 5-hydroxyguaiacyl monomer could be detected in the DFRC products. These facts imply that the DFRC method leaves the benzodioxane structures fully intact, suggesting that the method might therefore be useful as an analytical tool for determining benzodioxane structures that are linked by β-O-4 ethers. Using a modified DFRC procedure, we report here on results that provide further evidence for the existence of benzodioxane structures in lignins from COMT-deficient plants, that 5-OH-CA is behaving as a rather ideal monolignol that can be integrated into plant lignins, and demonstrate the usefulness of the DFRC method for determining these benzodioxane structures.Open in a separate windowFigure 2.Synthesis of benzodioxane DFRC products 12 (see later in Fig. 6 for their structures). i, NaH, THF. ii, Pyrrolidine. iii, 1g or 1s, benzene/acetone (4/1, v/v). iv, DIBAL-H, toluene. v, Iodomethane-K2CO3, acetone. vi, Ac2O pyridine. 相似文献
13.
Charlotte?K. Colenso Yang Cao Richard?B. Sessions Jules?C. Hancox Christopher?E. Dempsey 《Biophysical journal》2014,107(10):L25-L28
Relaxation of a hERG K+ channel model during molecular-dynamics simulation in a hydrated POPC bilayer was accompanied by transitions of an arginine gating charge across a charge transfer center in two voltage sensor domains. Inspection of the passage of arginine side chains across the charge transfer center suggests that the unique hydration properties of the arginine guanidine cation facilitates charge transfer during voltage sensor responses to changes in membrane potential, and underlies the preference of Arg over Lys as a mobile charge carrier in voltage-sensitive ion channels.The response of voltage-sensitive ion channels to changes in membrane potential is mediated by voltage sensor domains (VSD) containing a transmembrane helical segment (S4) with a repeating motif of positively charged and hydrophobic amino acids (Fig. 1) (1,2). Changes in membrane potential drive the S4 helix through the membrane plane with the charged side chains (largely arginine) on S4 swapping Glu/Asp carboxylate partners that lie on less mobile elements of the VSD (2). Movement of S4 is coupled to the ion-conducting pore to transmit changes in membrane potential to channel gating (3).Open in a separate windowFigure 1Structures of the VSD of membrane domains before MD in a POPC bilayer. The S2 (pink) and S4 (blue) helices of the VSD of the hERG model (A) and Kv1.2/2.1 chimera structure (B) are highlighted. (C) Sequence alignment of S2 and S4 among homologous voltage-sensitive K+ channels.The VSD charge-pairing motif of K+ and Na+ channels is best represented in VSD states at zero membrane potential (S4 helix up) for which crystal structures exist for Kv1.2 (4), Kv1.2/2.1 chimera (5), and Nav channels (6,7). In these states, positively charged residues on the intra- and extracellular sections of the S4 helix are separated by a hydrophobic charge-transfer center (CTC) (1) or plug (8) containing a highly conserved Phe residue (Fig. 1). This plug restricts water incursion across the VSD, focusing the electric field across a narrow region near the bilayer center. In voltage-driven transitions between S4 down- and up-states, positively charged S4 side chains move across the CTC.The ether-à-go-go (eag) and eag-related family of voltage-sensitive K+ channels likely share similar charge pairing interactions with VSDs in other channels (9,10). However, eag VSDs contain an extra negative charge on S2 (underlined in Fig. 1
C) so that in hERG, Asp residues (D460 and D466) lie approximately one helical turn above and below the conserved charge-transfer center Phe (F463) (Fig. 1). This eag-specific motif might be expected to facilitate transfer of Arg side chains through the CTC and to stabilize the voltage sensor (VS) in the up state. We recently described an open state (VS-up) hERG model built on the crystal structure template of the Kv1.2/2.1 chimera and molecular-dynamics (MD) simulation of this model in a hydrated POPC bilayer (11). We have inspected an extended version of this simulation and identified transitions of a gating charge into the CTC despite the absence of a membrane potential change. These transitions are absent in equivalent MD simulations of the chimera structure in a POPC bilayer.Fig. 1 shows a single VS from starting structures of the hERG model and the chimera structure in a hydrated POPC bilayer, after restrained MD to anneal the protein-lipid interface (see Methods in the Supporting Material). Because the hERG model is constructed on the chimera structure according to the alignment in Fig. 1 the pattern of pairing between S4 charges and acidic VS side chains is equivalent in the hERG model and chimera structure.The arrangement of charge-paired side chains remains constant during MD in all subunits of the chimera (e.g., Fig. 2
E and see Fig. S2 in the Supporting Material). However, in two subunits of the hERG model the R534 side chain moves toward the extracellular side of the bilayer, sliding into the CTC to form a charge interaction with the extra Asp residue (D460 in hERG) that lies just above F463 (Fig. 2, A–C). This transition is facilitated by changes in side-chain rotamers of R534 and F463 as the planar Arg guanidine group rotates past the F463 ring, and the availability of D460 as a counterion for the R534 guanidine (Fig. 2). Movement of an Arg guanidine past the Phe side chain of the CTC is similar to that described in steered MD of an isolated VS domain (12).Open in a separate windowFigure 2Movement of the R534 side chain across the CTC in chain a of the hERG model simulation (A). Similar transitions are observed in chains a and b (panels B and C), but not chains c (D) or d (not shown), where the R534 side chain remains close to D466. In all subunits of the Kv1.2/2.1 chimera simulation, charge pairing of the starting structure (Fig. 1B) was maintained throughout (e.g., panel E and see Fig. S2 in the Supporting Material). (Black and blue lines) Distances from the Arg CZ or Lys ε atom to the two O atoms, respectively, of Asp or Glu.Mason et al. (13) have shown, using neutron scattering, that the low charge density guanidine cation (Gdm+) corresponding to the Arg side chain is poorly hydrated above and below the molecular plane. This property may underlie the universal preference for Arg (over Lys) in voltage sensor charge transfer. Although the poorly-hydrated surfaces of Gdm+ interact favorably with nonpolar (especially planar) surfaces (14,15), Gdm+ retains in-plane hydrogen bonding (13). In the transition of R534 across the CTC, in-plane solvation of the guanidine side chain is provided initially by D466, D501, and water molecules below the CTC, and during and after the transition by D501 and D460 side chains and waters above the CTC (Fig. 3, A and B). Complete transfer of the R534 side chain across the CTC was not observed, but would be expected to involve movement of the guanidine group away from H-bonding distance with D501.Open in a separate windowFigure 3In-plane solvation of R534 guanidine in the charge transfer center during the hERG model MD (A). (Dotted lines) H-bond distances of <2.5 Å. The right-hand group consists of top-down (B) and end-on (C) views of the distribution of oxygen atoms around the side chain of hERG R534 at 20-ns intervals during MD (subunit a). (D) End-on view of equivalent atom distributions around the K302 side chain during the Kv1.2/2.1 chimera MD (subunit c). (Red spheres, water O; pink, Asp OD1 and OD2; purple:, Glu OE1 and OE2.)The atom distribution around the R534 side chain during MD (Fig. 3, B and C) conforms to the experimental Gdm+ hydration structure (13), with H-bonding to waters and side-chain Asp O atoms exclusively in the guanidine plane. The passage of Gdm+ through the CTC is facilitated by the hydrophobic nature of Gdm+ above and below the molecular plane (13), which allows interaction with the nonpolar groups (especially F463) in the CTC (Fig. 3
A and see Fig. S3). This contrasts with the solvation properties of the Lys amino group (e.g., K302 of the Kv1.2/2.1 chimera (Fig. 1), which has a spherical distribution of H-bonding and charge-neutralizing oxygen atoms (Fig. 3
D and see Fig. S4).To further test these interpretations, we ran additional MD simulations of the isolated hERG VS domain model and an R534K mutant in a hydrated POPC bilayer. Again, the R534 side chain entered the CTC in the wild-type model simulation whereas the K534 side chain did not (see Fig. S5). Inspection of the atom distributions in Fig. 3
D (and see Fig. S4) indicates that the pocket below the conserved Phe of the CTC is particularly favorable for a Lys side chain, with waters and acidic side chains that satisfy the spherical solvation requirements of the terminal amino group, and nonpolar side chains that interact with the aliphatic part of the side chain.The occurrence of transitions of the R534 side chain through the CTC in the hERG model, in the absence of a change in membrane potential, indicates a relaxation from a less-stable starting structure. However, the path of the R534 side chain provides useful molecular-level insight into the nature of charge transfer in voltage sensors. How do these observations accord with broader evidence of charge transfer in voltage-sensitive channels in general, and hERG in particular? Studies with fluorinated analogs of aromatic side chains equivalent to F463 of hERG or F233 of the chimera indicate the absence of a significant role for cation-π interactions involving the CTC aromatic group in K+ and Nav channels, although a planar side chain is preferred in some cases (1,16). In hERG, F463 can be replaced by M, L, or V with small effects on channel gating (17), indicating that the hERG CTC requires only a bulky nonpolar side chain to seal the hydrophobic center of the VS and allow passage of the Arg side chain through the CTC. Both absence of requirement for cation-π interactions, and accommodation of nonplanar hydrophobic side chains in a functional hERG CTC, are broadly consistent with the interpretation that it is the poorly-hydrated nature of the Arg guanidine group above and below the molecular plane (together with its tenacious proton affinity (18)) that governs its role in carrying gating charge in voltage sensors.While the simulations suggest that R534 may interact with D460 in the open channel state, the possibility that the extra carboxylate side chain above the CTC might facilitate gating charge transfer is seemingly inconsistent with the slow activation of hERG, although hERG D460C does activate even more slowly than the WT channel (9). However, S4 movement in hERG occurs in advance of channel opening (19), and slow gating is partly mediated by interactions involving hERG cytoplasmic domains (20); thus, slow S4 movement may not be an inherent property of the hERG voltage sensor. Recent studies show that when hERG gating is studied at very low [Ca2+] (50 μM) and low [H+] (pH 8.0), the channel is strongly sensitized in the direction of the open state; this effect is reduced in hERG D460C (and hERG D509C) (10). These observations support a role for the extra hERG Asp residues in binding Ca2+ (and H+) (10), allowing the channel to be allosterically responsive to changes in pH and [Ca2+]. A true comparison of a hERG model with experimental channel gating might involve studies on a channel lacking cytoplasmic domains that modulate gating, and using conditions (high pH and low [Ca2+]) that leave the eag-specific Asp residues unoccupied. This could reveal the inherent current-voltage relationships and kinetics of the hERG voltage sensor. 相似文献
14.
The epigenetic phenomenon of genomic imprinting has motivated the development of numerous theories for its evolutionary origins and genomic distribution. In this review, we examine the three theories that have best withstood theoretical and empirical scrutiny. These are: Haig and colleagues'' kinship theory; Day and Bonduriansky''s sexual antagonism theory; and Wolf and Hager''s maternal–offspring coadaptation theory. These theories have fundamentally different perspectives on the adaptive significance of imprinting. The kinship theory views imprinting as a mechanism to change gene dosage, with imprinting evolving because of the differential effect that gene dosage has on the fitness of matrilineal and patrilineal relatives. The sexual antagonism and maternal–offspring coadaptation theories view genomic imprinting as a mechanism to modify the resemblance of an individual to its two parents, with imprinting evolving to increase the probability of expressing the fitter of the two alleles at a locus. In an effort to stimulate further empirical work on the topic, we carefully detail the logic and assumptions of all three theories, clarify the specific predictions of each and suggest tests to discriminate between these alternative theories for why particular genes are imprinted.The discovery of genomic imprinting, where the expression of an allele depends on its parental origin, motivated a diversity of theories attempting to explain its existence (Spencer and Clark, 2014). Three main theories have withstood scrutiny and are the focus of this review: Haig and colleagues'' kinship theory (Haig and Westoby, 1989; Haig, 2000a, 2004); Day and Bonduriansky''s (2004) sexual antagonism theory (see also Bonduriansky, 2007); and Wolf and Hager''s (2006) maternal–offspring coadaptation theory (see also Wolf and Hager, 2009; Wolf, 2013). Although these theories rest on different logic and fundamental assumptions, they share a critical common feature: some process creates a selective asymmetry between the maternally and paternally inherited allelic copies at a locus that causes selection to favor differential expression of the alleles (typically silencing of one of the copies) (Figures 1, ,2,2, ,33).Open in a separate windowFigure 1The kinship theory of genomic imprinting has two prerequisites: first, epigenetic marks that differentiate matrigenes from patrigenes; second, a difference in the relatedness of matrigenes and patrigenes to the social group. (a) The social group in the example depicted is a single litter of offspring, and multiple mating produces a relatedness asymmetry between half-siblings. The relatedness for matrigenes is ½ and the relatedness for patrigenes is 0. (Other sources of relatedness asymmetry are possible—e.g., sex-biased dispersal or high fitness variance in one sex—and social interactions are not limited to the juvenile period only). (b) The kinship theory envisions kin selection acting independently on genes of maternal and paternal origin and solves for the evolutionarily stable gene expression strategy for matrigenes and patrigenes. (c) For genes where the matrigenic allele''s optimum expression level is higher than that of the patrigene''s (e.g., a fetal growth inhibitor), the kinship theory predicts silencing of the patrigenic allele; for genes with the opposite effect (e.g., a fetal growth enhancer), the prediction is for patrigenic expression.Open in a separate windowFigure 2(a, b) The sexual antagonism theory of genomic imprinting starts with sexually antagonistic selection, which produces different allele frequencies, shown as pie charts, for genes of maternal and paternal origin. (c, d) Natural selection favors individuals that are able to express the fitter of the two alleles at a locus, which for males will be the patrigenic allele and for females will be the matrigenic allele. (In addition, the sexual antagonism theory may predict matrigenic or patrigenic expression in both sexes, such that the expressed allele derives from the parental sex that experiences stronger selection pressure. This scenario is not depicted).Open in a separate windowFigure 3(a) The maternal–offspring coadaptation theory of genomic imprinting relies on the correlation of genes in the mother and genes of maternal origin in the offspring (shown in light blue). (b) Fitness of offspring is determined by the interaction (shown in dark purple) between the phenotypes of mothers and offspring. (c) Imprinted silencing of the patrigenic allele can be favored for either of two reasons, depending on the genetic architecture of the interacting phenotypes. First, when a single gene governs the interaction and phenotypic matching between mothers and their offspring produces high fitness, then silencing of the patrigenic allele is beneficial to offspring because it raises the probability of producing a match. Second, if different loci are involved in the phenotypic interaction, past correlational selection will have produced a covariance between them, generating haplotypes with combinations of alleles that interact well together. (N.B. This multi-locus interaction is not depicted in the figure.) The offspring is more likely to inherit from its mother an allele that interacts well with the alleles in the mother''s genotype. This also favors the imprinted silencing of the patrigenic allele because it raises the probability that the offspring expresses an allele that makes for a good interaction with the maternal phenotype.Here we provide an overview of the fundamental logic and critical assumptions of these models. We then derive predictions that can be used to distinguish between theories. In doing so, we also highlight ambiguities in and overlap between the predictions they make, with a goal of motivating further research. In addition, we suggest some areas for future work that will test some of these predictions. 相似文献
15.
George?W. Ashdown Andrew Cope Paul?W. Wiseman Dylan?M. Owen 《Biophysical journal》2014,107(9):L21-L23
We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form of velocity histograms and flow vector maps. The flow was found to be retrograde and radially directed throughout the periphery of T-cells during synapse formation.Many biological processes are now being visualized with the use of superresolution fluorescence microscopy techniques. However, localization-based techniques primarily rely on fixed or slow moving samples to permit the collection of structural information. The 10-fold gains in resolution afforded by these superresolution techniques are usually possible through sacrificing the factors that originally made microscopy such a powerful tool: the ability to image live cells. In the case of stimulated emission depletion imaging, the scanning approach associated with this technique may fail to detect faster molecular events when imaging whole cellular regions.Structured illumination microscopy (SIM) is an alternative to these methods (1). It increases the resolution of conventional fluorescence microscopy twofold; it has the advantage of using a wide-field system, providing fast acquisition speeds of whole cells with relatively low laser powers; and it is compatible with standard fluorophores. By using a physical grating to produce interference patterns from a laser, periodic illumination is created. This patterned illumination causes information from higher spatial frequencies to be downmodulated (i.e., shifted) into the optical transfer function (support region) of the lens, resulting in higher-resolution spatial information being captured than is ordinarily obtainable.To quantify the directional motion of intracellular molecules, spatiotemporal image correlation spectroscopy (STICS (2)) was applied. Using spatial image correlation in time, STICS measures the similarity of pixels with those surrounding in lagging frames via a correlation function. The correlation function provides information on both flow velocities and directionality, while discounting static structures through the immobile object filter, achieved by subtracting a moving average of pixel intensities.The formation of an immunological synapse between T cells and antigen-presenting cells is a process requiring many dynamic (3) and subdiffraction-limited clustering events (4–6) to take place. The polymerization of actin is important for the spreading of cells over their target antigen-presenting cells (7), as well as cell mobility and migration (8). Retrograde flow of densely meshed cortical actin is observed at the basal membrane of synapse-forming T cells, where it may have a role in the corralling and clustering of signaling molecules at the plasma membrane (9), as well as at the leading edge of migrating cells (10). Filamentous actin is an extremely dynamic (7), densely packed, and thin (7-nm) structure (11,12).Here, we perform STICS on SIM data acquired on a total internal reflection fluorescence (TIRF) microscope system, which generated an evanescent field of 75-nm depth for excitation. To our knowledge, this is the first demonstration of an image correlation approach to quantify molecular dynamics on subresolution length scales using wide-field microscopy. To demonstrate the technique, we analyze two-dimensional actin flows in CD4+ T cells during immunological synapse formation, performed after cross-linking of antigen T cell receptors on a coverslip coated with specific antibodies.Fig. 1
a shows a schematic of the TIRF SIM setup. Excitation light (488 nm) passes through a polarizing module and then a phase-grating block, producing diffracted beams. These are then passed through a diffraction filter module to isolate the −1 and +1 order laser beams. These first-order laser beams are angled through the objective to produce total internal reflection conditions at the glass-water interface. The two evanescent waves interfere at the sample, producing structured illumination. The setup then produces lateral and rotational shifts through three orientations, producing nine raw images containing higher spatial frequencies than can normally be acquired by an objective using standard light microscopy. Fig. 1
b demonstrates the increased resolution obtained from TIRF SIM. Shown are the collected Fourier frequencies compared to those of a conventional microscope (dotted red line). Resolution was also measured using sparse 100-nm diameter fluorescent beads. Fig. 1
c shows a magnified image of these beads from which a line profile was obtained (yellow arrow). The full width at half-maximum of this profile (Fig. 1
d) gives a lateral resolution for the system of 120 nm.Open in a separate windowFigure 1(a) Schematic of the TIRF SIM setup. (b) Demonstration of the doubling of spatial resolution of collected frequencies through a Fourier transform (superimposed red circle demonstrating regular spatial frequency limits). (c) SIM reconstructed image of 100-nm bead (scale bar 0.5 μm). (d) (Plotted line) Bead showing full width at half-maximum of 120 nm.We then applied STICS analysis to quantify actin flow in T cell synapses acquired using TIRF SIM (Fig. 2). Fig. 2
a shows a schematic of the STICS analysis. From the raw data, immobile objects are first filtered by subtracting a moving average of the pixel values. Vector maps were obtained from correlation analysis of the time-series as previously published in Hebert et al. (2) and Brown et al. (13). Fig. 2
b shows a reconstructed TIRF SIM image of a mature T cell immunological synapse, representative of a time-point derived from the time series acquired at 1.28 fps (see Movie S1 in the Supporting Material). From this reconstructed image, two representative regions have been selected. In these regions, pseudo-colored actin flow vectors are overlaid onto the fluorescence intensity image. These range in magnitude from 0.01 μm/min (blue) to 5.61 μm/min (red). It can be observed that all flow vectors are directed radially toward the synapse center. A histogram of this flow is shown in Fig. 2
c. The histogram shows a peak retrograde flow velocity of 1.91 ± 1.27 μm/min. These data are representative of n = 7 T-cell synapses imaged by TIRF SIM.Open in a separate windowFigure 2(a) STICS analysis, performed by isolating mobile from immobile structures through a moving average filter (i) and binning a subset of pixels into blocks of superpixels (ii); the STICS software correlates spatial fluorescence fluctuations through time (iii). The code then outputs vector maps showing directionality and flow velocities. (b) TIRF SIM image of actin flow in a T cell 5 min after contact with a stimulatory coverslip. (Zoomed regions) Retrograde actin flow at the synapse periphery. (c) Histograms showing flow speed statistics of vectors from T-cell synapses (n = 7). 相似文献
16.
17.
18.
Teleost fishes are the most species-rich clade of vertebrates and feature an overwhelming
diversity of sex-determining mechanisms, classically grouped into environmental and
genetic systems. Here, we review the recent findings in the field of sex determination in
fish. In the past few years, several new master regulators of sex determination and other
factors involved in sexual development have been discovered in teleosts. These data point
toward a greater genetic plasticity in generating the male and female sex than previously
appreciated and implicate novel gene pathways in the initial regulation of the sexual
fate. Overall, it seems that sex determination in fish does not resort to a single genetic
cascade but is rather regulated along a continuum of environmental and heritable
factors.IN contrast to mammals and birds, cold-blooded vertebrates, and among them teleost fishes in
particular, show a variety of strategies for sexual reproduction (Figure 1), ranging from unisexuality (all-female species) to
hermaphroditism (sequential, serial, and simultaneous, including outcrossing and selfing
species) to gonochorism (two separate sexes at all life stages). The underlying phenotypes are
regulated by a variety of sex determination (SD) mechanisms that have classically been divided
into two main categories: genetic sex determination (GSD) and environmental sex determination
(ESD) (Figure 2).Open in a separate windowFigure 1Reproductive strategies in fish. Fish can be grouped according to their reproductive
strategy into unisexuals, hermaphrodites, and gonochorists. Further subdivisions of these
three categories are shown with pictures of species exemplifying the strategies. Fish
images: Amphiprion clarkii courtesy of Sara Mae Stieb;
Hypoplectrus nigricans courtesy of Oscar Puebla; Scarus
ferrugineus courtesy of Moritz Muschick; Astatotilapia burtoni
courtesy of Anya Theis; Poecilia formosa and Kryptolebias
marmoratus courtesy of Manfred Schartl; Trimma sp. courtesy of
Rick Winterbottom [serial hermaphroditism has been described in several species of the
genus Trimma (Kuwamura and Nakashima
1998; Sakurai et al.
2009; and references therein)].Open in a separate windowFigure 2Sex-determining mechanisms in fish. Sex-determining systems in fish have been broadly
classified into environmental and genetic sex determination. For both classes, the
currently described subsystems are shown.Environmental factors impacting sex determination in fish are water pH, oxygen concentration,
growth rate, density, social state, and, most commonly, temperature (for a detailed review on
ESD see, e.g., Baroiller et
al. 2009b and Stelkens and Wedekind
2010). As indicated in Figure 2, GSD systems
in fish compose a variety of different mechanisms and have been reviewed in detail elsewhere
(e.g., Devlin and Nagahama 2002;
Volff et al. 2007).The GSD systems that have received the most scientific attention so far are those involving
sex chromosomes, which either may be distinguishable cytologically (heteromorphic) or appear
identical (homomorphic). In both cases, one sex is heterogametic (possessing two different sex
chromosomes and hence producing two types of gametes) and the other one homogametic (a
genotype with two copies of the same sex chromosome, producing only one type of gamete). A
male-heterogametic system is called an XX-XY system, and female-heterogametic systems are
denoted as ZZ-ZW. Both types of heterogamety exist in teleosts and are even found side by side
in closely related species [e.g., tilapias (Cnaani et al. 2008), ricefishes (Takehana et al. 2008), or sticklebacks (Ross et al. 2009)]; for more details on the phylogenetic
distribution of GSD mechanisms in teleost fish, see Mank
et al. (2006). Note that sex chromosomes in fish are mostly
homomorphic and not differentiated (Ohno 1974), which
is in contrast to the degenerated Y and W chromosomes in mammals (Graves 2006) and birds (Takagi and
Sasaki 1974), respectively. This is one possible explanation for the viable
combination of different sex chromosomal systems within a single species or population of fish
(Parnell and Streelman 2013) and could be a
mechanistic reason why sex chromosome turnovers occur easily and frequently in this group
(Mank and Avise 2009). Additionally, fish can have
more complex sex chromosomal systems involving more than one chromosome pair (see Figure 2). Even within a single fish species, more than two
sex chromosomes may occur at the same time, or more than two types of sex chromosomes may
co-exist in the same species (Schultheis et
al. 2006; Cioffi et al.
2013), which can sometimes be due to chromosome fusions (Kitano and Peichel 2012).Detailed insights on the gene level for GSD/sex chromosomal systems are currently available
for only a limited number of fish species, and all but one of these cases involve a rather
simple genetic system with male heterogamety and one major sex determiner (see below). The
only exception is the widely used model species zebrafish (Danio rerio),
which has a polyfactorial SD system implicating four different chromosomes (chromosomes 3, 4,
5, and 16) (Bradley et al. 2011;
Anderson et al. 2012) and also
environmental cues (Shang et al.
2006).In this review, we focus on newly described genetic sex-determining systems and possible
mechanisms allowing their emergence in fishes, which are the most successful group of
vertebrates with ∼30,000 species. 相似文献
19.
A novel lachrymatory factor synthase (LFS) was isolated and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The enzyme is a heterotetrameric glycoprotein comprised of two α-subunits (68.8 kD each), one γ-subunit (22.5 kD), and one δ-subunit (11.9 kD). The two α-subunits are glycosylated and connected by a disulfide bridge. The LFS has an isoelectric point of 5.2. It catalyzes the formation of a sulfine lachrymator, (Z)-phenylmethanethial S-oxide, only in the presence of P. alliacea alliinase and its natural substrate, S-benzyl-l-cysteine sulfoxide (petiveriin). Depending on its concentration relative to that of P. alliacea alliinase, the LFS sequesters, to varying degrees, the sulfenic acid intermediate formed by alliinase-mediated breakdown of petiveriin. At LFS:alliinase of 5:1, LFS sequesters all of the sulfenic acid formed by alliinase action on petiveriin, and converts it entirely to (Z)-phenylmethanethial S-oxide. However, starting at LFS:alliinase of 5:2, the LFS is unable to sequester all of the sulfenic acid produced by the alliinase, with the result that sulfenic acid that escapes the action of the LFS condenses with loss of water to form S-benzyl phenylmethanethiosulfinate (petivericin). The results show that the LFS and alliinase function in tandem, with the alliinase furnishing the sulfenic acid substrate on which the LFS acts. The results also show that the LFS modulates the formation of biologically active thiosulfinates that are downstream of the alliinase in a manner dependent upon the relative concentrations of the LFS and the alliinase. These observations suggest that manipulation of LFS-to-alliinase ratios in plants displaying this system may provide a means by which to rationally modify organosulfur small molecule profiles to obtain desired flavor and/or odor signatures, or increase the presence of desirable biologically active small molecules.Lachrymatory factor synthase (LFS) is the term coined to refer to the recently discovered enzyme shown to catalyze the formation of the sulfine responsible for the lachrymatory effect of onion (Allium cepa), (Z)-propanethial S-oxide (PTSO; Imai et al., 2002). Until the discovery of the onion LFS, the formation of the onion lachrymatory factor (LF) was thought to be mediated by only a single enzyme, onion alliinase. Alliinases, which are pyridoxal 5′-P (PLP)-dependent Cys sulfoxide lyases most often found in members of the Allium genus, catalyze the breakdown of Cys sulfoxide derivatives to yield fleeting sulfenic acid intermediates and α-aminoacrylic acid (Scheme 1; Block, 1992; Shimon et al., 2007). Once formed, the sulfenic acids are most often observed to spontaneously condense with loss of water to form thiosulfinates, whereas the α-aminoacrylic acid is further hydrolyzed with loss of ammonia to form pyruvate. The S-substituted Cys sulfoxides that are acted upon by alliinases differ from one another by the identity of the sulfur-bound R group. In Allium plants, the R groups are alk(en)yl, with R = methyl and 2-propenyl appearing in large quantities in garlic (Allium sativum) and R = methyl and (E)-1-propenyl preponderating in onion (Scheme 1). The Cys sulfoxide that serves as the precursor of the onion lachrymator is (E)-S-(1-propenyl)-l-Cys sulfoxide (isoalliin). It is structurally distinct from other naturally occurring S-substituted Cys sulfoxides so far reported in that it is α,β-unsaturated. This structural feature affords its corresponding 1-propenylsulfenic acid (PSA) the possibility of undergoing a [1,4]-sigmatropic rearrangement that, in principle, would furnish the onion lachrymator, PTSO. Indeed, the formation of the onion lachrymator was proposed to occur by such a mechanism (Scheme 2; Block, 1992). Thus, it was surmised that were the α,β-unsaturation to be absent in the precursor S-substituted Cys sulfoxide, the [1,4]-sigmatropic rearrangement that would lead to sulfine formation could not occur. Consequently, it was not surprising that other S-substituted Cys sulfoxides constitutively present in garlic, onion, and other alliinase-containing plants, but devoid of this α,β-unsaturation in the sulfur-bound R group, did not themselves yield lachrymators on plant tissue wounding. It has since been discovered, however, that formation of the onion lachrymator is not catalyzed by onion alliinase, but instead by a novel class of enzyme—LFS. Imai et al. (2002) observed that although a crude preparation of onion alliinase yielded both the LF and the corresponding thiosulfinate, the protein fraction with lachrymator-forming ability could be completely separated from that with alliinase activity by passing the crude onion protein preparation through a hydroxyapatite column. The LFS was subsequently purified and shown to be highly substrate specific, producing the LF from only (E)-S-(1-propenyl)-l-Cys sulfoxide (isoalliin), which occurs constitutively in onion. Interestingly, the LF was detected only when three components, namely, the purified onion alliinase, isoalliin, and the onion LFS, were present in the reaction mixture simultaneously (Imai et al., 2002). Omission of the LFS from the reaction mixture resulted in an increased yield of thiosulfinates, but no LF. Although the complete cDNA sequence of the onion LFS has been determined (Imai et al., 2002), to our knowledge, full biochemical characterization of the enzyme has yet to be reported.Open in a separate windowScheme 1.Alliinase-mediated formation of thiosulfinates from Cys sulfoxide precursors (Block, 1992; Shimon et al., 2007). Alliin is S-allyl-l-Cys sulfoxide, isoalliin is (E)-S-(1-propenyl)-l-Cys sulfoxide, methiin is S-methyl-l-Cys sulfoxide, and propiin is S-propyl-l-Cys sulfoxide.Open in a separate windowScheme 2.Mechanism advanced by Block (1992) to account for formation of the onion lachrymator, PTSO. Alliinase-bound PLP forms a Schiff base with bound isoalliin. General base catalysis at the active site yields an α,β-unsaturated sulfenic acid that can undergo a [1,4]-sigmatropic rearrangement to furnish the sulfine.In the course of our studies on the organosulfur chemistry of non-Allium plants, we isolated and characterized the S-benzyl-l-Cys sulfoxides (petiveriins) and S-(2-hydroxyethyl)-l-Cys sulfoxides (2-hydroxyethiins) from the Amazonian medicinal plant Petiveria alliacea (Fig. 1; Kubec and Musah, 2001; Kubec et al., 2002). These compounds are S-substituted Cys sulfoxide derivatives with R = benzyl and 2-hydroxyethyl, respectively, that, to our knowledge, had never before been isolated from plants. We showed that, as has been observed in garlic and onion, symmetrical and mixed thiosulfinate derivatives of the corresponding petiveriin and 2-hydroxyethiin precursors could be extracted with ether solvent (Fig. 1; Kubec et al., 2002) upon root tissue disruption. We have also shown that an alliinase that mediates the transformation of the petiveriins and 2-hydroxyethiins to their corresponding thiosulfinates is present in P. alliacea (Musah et al., 2009). Interestingly, while working with P. alliacea root extracts, we noted the presence of a potent lachrymator that we subsequently determined to be a sulfine—(Z)-phenylmethanethial S-oxide (PMTSO; Fig. 2; Kubec et al., 2003). However, the biochemical precursor of PMTSO and the pathway(s) leading to its formation upon disruption of P. alliacea tissue remain to be determined. Given that the onion LF (PTSO), whose formation is mediated by an LFS, is also a sulfine, we were prompted to investigate the possibility of the presence of a LFS in P. alliacea. In this report, we describe our confirmation of the existence of a LFS in P. alliacea, and detail biochemical characterization of this novel class of enzymes.Open in a separate windowFigure 1.Cys sulfoxides and their corresponding thiosulfinate derivatives isolated from the Amazonian medicinal plant P. alliacea. The breakdown of the Cys sulfoxides is mediated by P. alliacea alliinase.Open in a separate windowFigure 2.Lachrymatory sulfine isolated from P. alliacea. 相似文献