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本实验旨在研究一种新的CO2激光心肌打孔方法改善急性心肌缺血。8只犬结扎冠状动脉左前降支获得急性心肌缺血动物模型,在缺血区随机分为A、B二区,A区不打孔作为自身空白对照,B区在心脏跳动情况下被激光打孔,在打孔时将滤波片放在光关节臂和心脏表面之间。结果:(1)心外膜电图,∑ST,打孔后30分钟,A、B二区差值具有显著意义(P<0.020);NST,打孔后20分钟,A、B二区比较有显著差异(P<0.005)。(2)病理学,冰冻切片H.E染色,激光孔没有碳化层,水肿层仅为50um,激光孔和周围血管相通,血管内可见血液的各种有形成份。实验表明,在形成急性心肌缺血情况下及时采用这种新的心肌打孔方法进行打孔能再造一套有效的血液供应系统,明显改善缺血程度,缩小缺血范围。 相似文献
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为临床研究和应用激光心肌血管重建术提供解剖学基础,本文在16只杂交犬的心肌梗塞模型上,行CO2激光心肌打孔。术后3或6个月取出心脏,观察不同时间未铸型及铸型激光心肌管道的形态特征。结果表明激光管道心内膜侧管径大于心外膜侧,管道多呈锥形。 相似文献
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本实验旨在研究激光心肌血管重建术二种打孔方法对激光孔远期通畅的影响。方法36只犬结扎左前降支获得急性心肌缺血动物模型,在缺血区随机分为A、B二区,A区采用“Mirhoseini”法,B区采用“滤波片”法打孔,术后4小时、0.5月、1个月、3个月、6个月取出心脏,通过病理学及微血管铸型判断激光孔是否通畅。结果术后6个月“Miehoseiri”法通畅率38%,“滤波片”法70%,术后36个月激光孔仍通畅。结论“滤波片”法优于“Mirhoseini”法,二种打孔方法对心肌缺血有长期改善作用。 相似文献
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为探讨CO2激光进行肺组织轻创手术效果,本实验采用10只离体猪肺,100WCO2激光进行直接照射;另用10只杂交犬在全麻、气管插管条件下进行激光照射,初步摸索出激光肺组织轻创伤手术步骤。实验犬饲养3 ̄6个月后处死,肺组织病理,HE染色,气化形成的空洞,8例空洞消失,2例有由纤维结缔组织增生形成腔壁的空腔。结果CO2激光肺组织轻创伤手术是一种安全、创伤小的手术,是一种可以融诊断、治疗;融激光直接气化 相似文献
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DOCA—Salt高血压大鼠模型的一种简易制备方法:DOCA硅胶管皮下埋入法 总被引:4,自引:0,他引:4
用外径4mm,内径2.50mm的硅胶管制成长25mm,管内填入DOCA100mg,管壁钻有10一14个直径约300μm微孔的药管,埋入雄性SD大鼠(140±9g)右下腹皮下,摘除一侧肾脏,术后喂1%盐水。埋管后3周即可形成高血压,埋管后8周大鼠的收缩压达23.3±0.37kPa。而DOCA皮下注射组大鼠(10mg/周)术后5周形成高血压,术后13周大鼠的收缩压达23.3±0.66kPa。两组升压曲线回归系数(1.295和0.692)之间的差异有极显著性意义(P<0.001)。对照鼠的收缩压一直保持在正常水平(16±0.16kPa)。与DOCA皮下注射法相比,皮下埋管法具有两个显著优点:(1)升压速率较快,升压幅度较大;(2)方法简便可靠,重复性好。 相似文献
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目的:了解临床常用功率密度的CO2激光治疗尖锐湿疣术中烟尘是否含有HPV DNA。方法:选择皮损较多的尖锐湿疣,病程在6个月以内的患者作为研究对象,分别用5W、10W、15W对应功率密度下的CO2激光汽化在体疣体组织,离光斑5cm或50cm处用吸尘器提吸烟尘于PBS中,经固定后观察烟尘的细胞形态学,并用HPV通用型引物作PCR检测烟尘是否含有HPV-DNA。结果:三种功率密度的烟尘主要由炭化颗粒组 相似文献
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CO2激光对女性外生殖系疾病的审美修复作用 总被引:1,自引:0,他引:1
应用CO2激光对56例女性外生殖系疾患的74个病灶地汽化治疗,效果显著,治愈率达100%,同时对处女膜发育异常,阴唇肥大及外有黑色素痣等进行了审美修复,并对术后伤口愈合及其功能恢复进行了随访,作者对CO2激光治疗外阴疾患和审美修复的有关问题提出了见解。 相似文献
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目的:观察应用Nd:YAG激光,消除人工晶体植入术后,影响视力的后囊膜皱褶之效果。方法:国产JYZ-1A型YAG激光眼科治疗机。用单脉冲能量0.35mJ-1.5mJ,治疗总能量11.2mJ64.5mJ击射晶体后囊膜皱褶深处,形成34mm孔径管亮区。 相似文献
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深圳湾大弹涂鱼种群结构与生殖特征 总被引:8,自引:0,他引:8
本文探讨了深圳湾大弹涂鱼种群结构和生殖特征。结果表明:该种群以1、2龄鱼组成为主,3龄鱼极少,表明种群结构简单,体长与体重的相关曲线方程为W=4.1618×10-5L2.7451(r=0.9576)。雌雄性比为3:2。一龄鱼开始性成熟。雌鱼性成熟最小生物学体长为62mm,雄鱼为59mm。个体绝对生殖力波动于1956—14053粒,平均7293粒;个体相对生殖力F/L:29.5—100.5粒/mm,平均79粒/mm;F/W:511.0—804.9粒/g,平均752粒/g。4—6月为繁殖盛期,7—8月为繁殖末期。雌性周年月平均成熟系数(GSI)变动范围为0.27%—4.36%。大弹涂鱼在一个生殖季节中只能产卵一次,属于一次性排卵类型。 相似文献
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缺氧对培养的肺动脉内皮细胞血管紧张素Ⅱ分泌的影响 总被引:2,自引:0,他引:2
缺氧是否通过影响血管内皮细胞的分泌功能而参与缺氧性肺动脉高压的发生尚不清楚。本实验动态观察了缺氧对培养的新生小牛内皮细胞(PAEC)的血管紧张素Ⅱ(ATⅡ)分泌的影响。结果发现:2.5%O2缺氧早期(1.5h),PAEC的ATⅡ分泌增加(P<0.01vs常氧组),缺氧后期与常氧组无明显差别;0%O2缺氧早期(1.5-6h),ATⅡ分泌明显降低(P<0.01vs常氧组及2.5%O2组),后期ATⅡ分泌明显增高(P<0.01vs常氧组及2.5%O2组);无论缺氧还是常氧条件下,NO供体SIN1显著抑制ATⅡ的分泌(P<0.01),而内源性NO抑制剂硝基精氨酸则明显促进ATⅡ分泌(P<0.01);0%O2缺氧24h后,PAEC细胞内cGMP含量明显降低(P<0.05)。上述结果表明缺氧可通过抑制PAEC的内源性NO产生而促进ATⅡ的分泌,PAEC自分泌的改变可能参与缺氧性肺动脉高压的发生过程。 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
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肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献
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H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献